AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011791

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011791 MtrunA17_Chr3g0085681 97.241 435 12 0 9 443 4 438 0.0 880
MsaG011791 MtrunA17_Chr3g0085361 95.143 350 16 1 94 443 4 352 0.0 684
MsaG011791 MtrunA17_Chr5g0396491 54.788 449 191 5 7 443 32 480 1.76e-169 484
MsaG011791 MtrunA17_Chr4g0038851 50.112 447 213 4 7 443 25 471 2.71e-159 458
MsaG011791 MtrunA17_Chr5g0447691 30.788 419 230 6 31 441 178 544 3.52e-61 207
MsaG011791 MtrunA17_Chr1g0159461 29.976 417 236 4 31 441 226 592 1.97e-59 204
MsaG011791 MtrunA17_Chr8g0376381 29.787 423 217 10 31 442 170 523 7.01e-59 201
MsaG011791 MtrunA17_Chr7g0224271 29.535 430 227 6 31 441 301 673 1.42e-58 203
MsaG011791 MtrunA17_Chr2g0319091 30.935 417 230 7 31 440 213 578 1.21e-55 194
MsaG011791 MtrunA17_Chr5g0445331 27.685 419 247 6 31 441 162 532 4.36e-55 191
MsaG011791 MtrunA17_Chr3g0125811 28.266 421 246 4 27 441 200 570 2.15e-54 190
MsaG011791 MtrunA17_Chr3g0101141 28.058 417 244 6 31 441 176 542 5.77e-54 188
MsaG011791 MtrunA17_Chr2g0306641 30.714 420 224 10 31 441 126 487 6.66e-53 184
MsaG011791 MtrunA17_Chr7g0245601 29.157 415 225 8 33 441 441 792 5.55e-52 187
MsaG011791 MtrunA17_Chr4g0076091 27.553 421 240 8 31 440 176 542 9.10e-48 172
MsaG011791 MtrunA17_Chr4g0057291 27.078 421 244 8 31 440 293 661 4.66e-46 169
MsaG011791 MtrunA17_Chr2g0290241 26.492 419 249 7 31 441 58 425 5.00e-46 164
MsaG011791 MtrunA17_Chr2g0328391 26.555 418 250 6 31 440 300 668 8.09e-45 165
MsaG011791 MtrunA17_Chr8g0357601 27.934 426 236 9 31 441 183 552 1.63e-44 163
MsaG011791 MtrunA17_Chr4g0031721 27.990 418 242 7 31 440 317 683 2.27e-44 164
MsaG011791 MtrunA17_Chr2g0328411 28.469 418 237 8 31 440 290 653 2.78e-44 164
MsaG011791 MtrunA17_Chr4g0031741 27.512 418 242 7 31 440 285 649 4.01e-43 160
MsaG011791 MtrunA17_Chr7g0238891 27.273 418 247 6 31 441 373 740 4.58e-42 158
MsaG011791 MtrunA17_Chr4g0031711 27.423 423 242 9 31 440 362 732 5.83e-42 158
MsaG011791 MtrunA17_Chr2g0328431 27.818 417 241 7 31 440 290 653 2.76e-41 155
MsaG011791 MtrunA17_Chr2g0328341 26.969 419 243 8 31 440 273 637 3.08e-41 155
MsaG011791 MtrunA17_Chr2g0328381 25.542 415 254 7 31 440 276 640 5.17e-41 154
MsaG011791 MtrunA17_Chr4g0057891 26.241 423 248 7 31 441 150 520 7.56e-41 152
MsaG011791 MtrunA17_Chr4g0031701 26.555 418 244 10 31 436 255 621 7.50e-40 151
MsaG011791 MtrunA17_Chr4g0038761 29.126 412 233 10 33 441 87 442 8.00e-40 148
MsaG011791 MtrunA17_Chr4g0031731 26.202 416 248 8 31 436 255 621 2.62e-39 149
MsaG011791 MtrunA17_Chr4g0039661 27.103 428 240 10 26 441 188 555 5.71e-36 139
MsaG011791 MtrunA17_Chr1g0183641 26.118 425 247 10 24 443 117 479 6.54e-36 138
MsaG011791 MtrunA17_Chr2g0294461 27.739 429 240 8 31 442 211 586 9.36e-36 139
MsaG011791 MtrunA17_Chr2g0294471 27.635 427 240 9 31 442 205 577 1.41e-35 138
MsaG011791 MtrunA17_Chr2g0328401 26.854 391 227 6 31 415 291 628 1.78e-35 139
MsaG011791 MtrunA17_Chr5g0401131 23.349 424 264 8 31 441 115 490 4.42e-32 127
MsaG011791 MtrunA17_Chr2g0328361 25.225 444 244 11 6 440 250 614 1.97e-31 127
MsaG011791 MtrunA17_Chr5g0424131 24.190 401 236 7 56 440 150 498 1.05e-28 118
MsaG011791 MtrunA17_Chr1g0200401 24.612 451 248 15 21 441 71 459 1.88e-28 117
MsaG011791 MtrunA17_Chr3g0087711 87.931 58 7 0 181 238 1 58 2.95e-28 106
MsaG011791 MtrunA17_Chr3g0110971 39.752 161 83 3 296 442 428 588 4.58e-28 117
MsaG011791 MtrunA17_Chr3g0110971 31.100 209 130 4 31 234 220 419 1.09e-27 115
MsaG011791 MtrunA17_Chr3g0114841 24.667 450 256 14 17 440 107 499 6.15e-28 115
MsaG011791 MtrunA17_Chr4g0053901 24.537 432 280 10 31 440 60 467 3.08e-26 110
MsaG011791 MtrunA17_Chr2g0328371 25.704 284 159 5 112 392 12 246 8.52e-26 105
MsaG011791 MtrunA17_Chr5g0401421 22.811 434 287 10 31 440 36 445 4.46e-24 103
MsaG011791 MtrunA17_Chr7g0236851 25.952 420 243 13 31 440 295 656 8.37e-24 104
MsaG011791 MtrunA17_Chr7g0270031 23.858 394 238 9 57 442 216 555 2.42e-23 102
MsaG011791 MtrunA17_Chr1g0194621 24.372 398 228 12 61 442 179 519 1.83e-19 90.9
MsaG011791 MtrunA17_Chr6g0468561 24.471 425 249 16 31 441 296 662 9.47e-19 89.0
MsaG011791 MtrunA17_Chr2g0329591 22.529 435 255 11 31 441 75 451 2.55e-18 87.0
MsaG011791 MtrunA17_Chr8g0383981 23.038 395 229 12 57 440 165 495 3.43e-17 84.0
MsaG011791 MtrunA17_Chr4g0012071 21.875 416 259 11 31 441 197 551 2.08e-16 81.6
MsaG011791 MtrunA17_Chr2g0323111 29.452 146 102 1 295 440 161 305 2.85e-16 79.3
MsaG011791 MtrunA17_Chr1g0207361 46.535 101 51 2 249 346 64 164 3.26e-16 76.3
MsaG011791 MtrunA17_Chr7g0230771 31.915 141 94 1 31 171 376 514 2.95e-11 65.9
MsaG011791 MtrunA17_Chr7g0230731 31.915 141 94 1 31 171 373 511 3.73e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011791 AT1G50420.1 49.330 448 216 4 7 443 34 481 9.47e-151 437
MsaG011791 AT1G66350.1 31.250 416 218 9 31 442 156 507 9.96e-63 211
MsaG011791 AT5G48150.1 30.324 432 237 5 16 441 117 490 8.90e-62 208
MsaG011791 AT5G48150.4 30.324 432 237 5 16 441 117 490 8.90e-62 208
MsaG011791 AT5G48150.2 30.324 432 237 5 16 441 117 490 8.90e-62 208
MsaG011791 AT5G48150.3 30.324 432 237 5 16 441 117 490 8.90e-62 208
MsaG011791 AT3G03450.1 29.009 424 228 9 31 442 184 546 8.77e-59 202
MsaG011791 AT1G50600.3 29.736 417 237 5 31 441 160 526 2.19e-57 197
MsaG011791 AT2G04890.1 30.583 412 233 6 31 441 54 413 3.70e-57 194
MsaG011791 AT1G50600.2 29.736 417 237 5 31 441 235 601 1.08e-56 197
MsaG011791 AT1G50600.1 29.736 417 237 5 31 441 231 597 1.14e-56 197
MsaG011791 AT1G14920.1 29.343 426 219 10 31 442 173 530 7.24e-55 191
MsaG011791 AT2G01570.1 28.504 421 229 8 31 442 225 582 2.81e-54 191
MsaG011791 AT4G17230.1 29.017 417 240 6 31 441 159 525 3.21e-52 184
MsaG011791 AT4G17230.2 29.017 417 240 6 31 441 159 525 3.21e-52 184
MsaG011791 AT3G54220.1 28.241 432 250 7 15 443 278 652 2.80e-51 183
MsaG011791 AT1G21450.1 29.009 424 244 7 25 441 220 593 1.79e-50 180
MsaG011791 AT1G63100.1 25.059 427 252 5 31 440 253 628 1.22e-44 165
MsaG011791 AT1G63100.2 25.059 427 252 5 31 440 278 653 1.38e-44 165
MsaG011791 AT2G29060.2 28.741 421 238 10 31 440 285 654 4.23e-43 161
MsaG011791 AT2G29060.1 28.741 421 238 10 31 440 322 691 5.52e-43 161
MsaG011791 AT1G07530.1 27.316 421 242 9 31 440 397 764 2.43e-42 159
MsaG011791 AT5G59450.1 28.504 421 241 8 31 441 228 598 2.71e-41 155
MsaG011791 AT3G46600.2 27.990 418 243 8 31 441 5 371 1.17e-38 144
MsaG011791 AT5G41920.1 27.644 416 237 9 31 441 44 400 1.65e-38 144
MsaG011791 AT3G46600.3 27.990 418 243 8 31 441 181 547 3.67e-37 143
MsaG011791 AT3G46600.1 27.990 418 243 8 31 441 213 579 7.99e-37 142
MsaG011791 AT1G55580.1 25.166 453 245 7 31 441 45 445 4.62e-36 138
MsaG011791 AT1G07520.2 25.117 426 245 9 34 440 158 528 1.30e-35 138
MsaG011791 AT1G07520.4 25.117 426 245 9 34 440 158 528 1.30e-35 138
MsaG011791 AT1G07520.3 25.117 426 245 9 34 440 323 693 3.79e-35 138
MsaG011791 AT1G07520.1 25.117 426 245 9 34 440 322 692 4.48e-35 138
MsaG011791 AT2G37650.1 26.077 418 250 7 31 441 348 713 5.02e-35 138
MsaG011791 AT2G37650.2 26.077 418 250 7 31 441 336 701 5.19e-35 138
MsaG011791 AT5G66770.1 27.187 423 237 9 31 441 221 584 2.31e-34 135
MsaG011791 AT2G29065.1 24.590 427 253 8 31 440 253 627 5.21e-32 129
MsaG011791 AT4G37650.1 23.112 437 256 12 31 441 147 529 9.19e-32 127
MsaG011791 AT3G50650.1 25.743 404 226 7 53 441 198 542 1.21e-31 127
MsaG011791 AT5G52510.1 23.843 432 248 9 31 441 269 640 2.27e-29 121
MsaG011791 AT5G17490.1 30.769 208 133 5 31 234 161 361 5.65e-29 119
MsaG011791 AT5G17490.1 38.000 150 90 2 295 442 369 517 9.64e-26 110
MsaG011791 AT3G49950.1 23.077 429 259 13 31 440 31 407 3.46e-24 104
MsaG011791 AT4G08250.1 22.717 427 262 12 28 440 104 476 6.89e-23 101

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAAGTTTGCTAATTAT+TGG 0.118308 3:-12219468 None:intergenic
CTGTATCGGCCTGGAAATTT+AGG 0.184893 3:+12220177 MsaT011791.1:CDS
GACAACCGAAGCAGGGATTT+TGG 0.226524 3:+12219425 MsaT011791.1:CDS
TCTGAATGGATGTAATTTAA+TGG 0.250687 3:+12219815 MsaT011791.1:CDS
ATGGATTCAAAGACTTAAAA+TGG 0.281483 3:+12220016 MsaT011791.1:CDS
AAACTCGAGAGCATGCTTCT+TGG 0.323417 3:+12219924 MsaT011791.1:CDS
TTAAGAAAGATCCCCATCTT+AGG 0.362039 3:-12219738 None:intergenic
TAATTGCTTGATTTGTGATC+AGG 0.367971 3:-12219227 None:intergenic
CCGCCCCATCTGAAAATTAC+AGG 0.369403 3:+12219357 MsaT011791.1:CDS
TATGACTATATCGCTGCAAT+AGG 0.370279 3:-12220091 None:intergenic
TTTAGGTGCTTCACCTAAGA+TGG 0.374066 3:+12219725 MsaT011791.1:CDS
ATGATTGTTTACTTGTTTGT+TGG 0.377997 3:+12220135 MsaT011791.1:CDS
AAGAGGTGATAATGCTGAAT+CGG 0.390322 3:-12219700 None:intergenic
GAATCATCACCTAAATTTCC+AGG 0.414889 3:-12220186 None:intergenic
AATTCCCTCAGCCAGTGATA+TGG 0.418979 3:-12218901 None:intergenic
TCTTGCCACTGATGATGAAA+TGG 0.424264 3:+12219566 MsaT011791.1:CDS
TTCAGATGGGGCGGACCTCC+CGG 0.424679 3:-12219348 None:intergenic
CACTCAATAAGAGGGATCAT+TGG 0.426274 3:-12218949 None:intergenic
ATTCAAAGACTTAAAATGGC+TGG 0.439982 3:+12220020 MsaT011791.1:CDS
CGAATCGATGGAACTTGAAA+AGG 0.443331 3:+12219251 MsaT011791.1:CDS
ATTATTGGATTGAACTGTAA+AGG 0.444269 3:-12219453 None:intergenic
TCAGTAATCACTAAAAGTTT+CGG 0.445565 3:-12219780 None:intergenic
TTTCATTTGACAACCGAAGC+AGG 0.447617 3:+12219417 MsaT011791.1:CDS
AAAGGAAAGGCATGAGAAGC+TGG 0.466170 3:+12219989 MsaT011791.1:CDS
AAAATCCAAAATCCCTGCTT+CGG 0.483217 3:-12219430 None:intergenic
TCATGAAAAGAAAGAAGTAT+TGG 0.489286 3:+12219383 MsaT011791.1:CDS
TGTGCAAAATGTGTTGCATC+TGG 0.490537 3:+12218970 MsaT011791.1:CDS
AGAGGTGATAATGCTGAATC+GGG 0.492776 3:-12219699 None:intergenic
TTAGGTGCTTCACCTAAGAT+GGG 0.494167 3:+12219726 MsaT011791.1:CDS
CACTAGGTTCGCCATATCAC+TGG 0.495093 3:+12218890 MsaT011791.1:CDS
GAAAACTTGCCTGTCAAGAC+AGG 0.495316 3:+12219501 MsaT011791.1:CDS
GAACAGGAATCAAATCTGAA+TGG 0.501626 3:+12219801 MsaT011791.1:CDS
ATTTATCCATTGCGCTGGCT+CGG 0.503400 3:-12219301 None:intergenic
CAACTCAGCTGTTATGCACT+AGG 0.503968 3:+12218874 MsaT011791.1:CDS
ATTTCATCACCTCATGGTAA+TGG 0.506040 3:+12219036 MsaT011791.1:CDS
TGGTAATGGTGTACAAAGAA+TGG 0.507522 3:+12219050 MsaT011791.1:CDS
TTGAGAGCTTTGTATACGCC+AGG 0.508327 3:-12219120 None:intergenic
ACAGCGTCTCCTGTCTTGAC+AGG 0.513933 3:-12219510 None:intergenic
TGGCAAGAAGAGAATGCAGC+TGG 0.515279 3:-12219551 None:intergenic
ACTTATTTCAGTGAAGCACT+TGG 0.520928 3:+12219075 MsaT011791.1:CDS
TCAAGCAATTATCGAATCGA+TGG 0.531832 3:+12219239 MsaT011791.1:CDS
GATGAAATGGTCTCCTCAGC+AGG 0.533946 3:+12219579 MsaT011791.1:CDS
ATCAGATTTATCCATTGCGC+TGG 0.534751 3:-12219306 None:intergenic
GGCATGAGAAGCTGGAGCAA+TGG 0.535021 3:+12219997 MsaT011791.1:CDS
ACAAACTTTAAAGAAACGAC+CGG 0.538739 3:+12219329 MsaT011791.1:CDS
AGGTACCTTTGAGTTACAAT+GGG 0.540375 3:+12220051 MsaT011791.1:CDS
TTGTGAGGGAGTCGATAGAA+AGG 0.541472 3:+12219971 MsaT011791.1:CDS
AAGGTACCTTTGAGTTACAA+TGG 0.544427 3:+12220050 MsaT011791.1:CDS
ATTGCGCTGGCTCGGAACAA+TGG 0.551742 3:-12219293 None:intergenic
TTTAATGGAGAGAATCGATC+GGG 0.556728 3:+12219830 MsaT011791.1:CDS
TCTCAGATTTCATCACCTCA+TGG 0.558265 3:+12219030 MsaT011791.1:CDS
TTCATTTGACAACCGAAGCA+GGG 0.560755 3:+12219418 MsaT011791.1:CDS
TACCTTTGAGTTACAATGGG+AGG 0.570596 3:+12220054 MsaT011791.1:CDS
TATGCATAGAGCAGCGCACA+TGG 0.570662 3:+12219617 MsaT011791.1:CDS
AAGAACATCATTACTTGTGA+GGG 0.575874 3:+12219957 MsaT011791.1:CDS
ATCCTCCCATTGTAACTCAA+AGG 0.576632 3:-12220056 None:intergenic
AAAGAACATCATTACTTGTG+AGG 0.578365 3:+12219956 MsaT011791.1:CDS
TAAAATGGCTGGATTTGTGA+AGG 0.583083 3:+12220031 MsaT011791.1:CDS
ATCGATGGAACTTGAAAAGG+TGG 0.592860 3:+12219254 MsaT011791.1:CDS
GATAGATGTCGCACCTGCTG+AGG 0.593900 3:-12219592 None:intergenic
GTTCGCCATATCACTGGCTG+AGG 0.598645 3:+12218896 MsaT011791.1:CDS
TAGGTGCTTCACCTAAGATG+GGG 0.607732 3:+12219727 MsaT011791.1:CDS
ATTTAATGGAGAGAATCGAT+CGG 0.609880 3:+12219829 MsaT011791.1:CDS
AAGATAGTTAAACATTTACC+TGG 0.616797 3:+12219102 MsaT011791.1:CDS
ATTGTTCCGAGCCAGCGCAA+TGG 0.617587 3:+12219295 MsaT011791.1:CDS
GAATTGAGATATGACTCTCA+TGG 0.618687 3:+12218919 MsaT011791.1:CDS
GAGCAGCGCACATGGGTCAG+AGG 0.620315 3:+12219625 MsaT011791.1:CDS
GGAGACCATTTCATCATCAG+TGG 0.644820 3:-12219571 None:intergenic
ATGCATAGAGCAGCGCACAT+GGG 0.647261 3:+12219618 MsaT011791.1:CDS
CAAACTTTAAAGAAACGACC+GGG 0.651089 3:+12219330 MsaT011791.1:CDS
GTCAGAGGACTTTCGCAGAG+TGG 0.652841 3:+12219640 MsaT011791.1:CDS
TTCGCCATATCACTGGCTGA+GGG 0.658373 3:+12218897 MsaT011791.1:CDS
AGGGAGTCGATAGAAAGGAA+AGG 0.670114 3:+12219976 MsaT011791.1:CDS
CTTTGTACACCATTACCATG+AGG 0.671867 3:-12219045 None:intergenic
ACTTTAAAGAAACGACCGGG+AGG 0.713179 3:+12219333 MsaT011791.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 12218871 12220202 12218871 ID=MsaG011791
Chr3 mRNA 12218871 12220202 12218871 ID=MsaT011791.1;Parent=MsaG011791
Chr3 exon 12218871 12220202 12218871 ID=MsaT011791.1.exon1;Parent=MsaT011791.1
Chr3 CDS 12218871 12220202 12218871 ID=cds.MsaT011791.1;Parent=MsaT011791.1
Gene Sequence

>MsaT011791.1

ATGCAACTCAGCTGTTATGCACTAGGTTCGCCATATCACTGGCTGAGGGAATTGAGATATGACTCTCATGGCTCAAATCCAATGATCCCTCTTATTGAGTGTGCAAAATGTGTTGCATCTGGAAGCATAAAAAATGCTGATATTGGACTTGAGTATATTTCTCAGATTTCATCACCTCATGGTAATGGTGTACAAAGAATGGTCACTTATTTCAGTGAAGCACTTGGATACAAGATAGTTAAACATTTACCTGGCGTATACAAAGCTCTCAATTCCTCGAAAATATCATCGTCTTCAGATGATATCCTTGTTCAAAAATACTTCTATGATCTGTGTCCTTTTTTGAAGTTTTCATACCTGATCACAAATCAAGCAATTATCGAATCGATGGAACTTGAAAAGGTGGTTCATATTATCGATCTCCATTGTTCCGAGCCAGCGCAATGGATAAATCTGATACAAACTTTAAAGAAACGACCGGGAGGTCCGCCCCATCTGAAAATTACAGGAATTCATGAAAAGAAAGAAGTATTGGAGCAAATGAGCTTTCATTTGACAACCGAAGCAGGGATTTTGGATTTTCCTTTACAGTTCAATCCAATAATTAGCAAACTTGAAGATGTCGATTTCGAAAACTTGCCTGTCAAGACAGGAGACGCTGTCGCAATTTCTTCTGTTCTCCAGCTGCATTCTCTTCTTGCCACTGATGATGAAATGGTCTCCTCAGCAGGTGCGACATCTATCAATATGCATAGAGCAGCGCACATGGGTCAGAGGACTTTCGCAGAGTGGCTAGAGAGAGACATGATCAATGCATATATCTTGAGTCCCGATTCAGCATTATCACCTCTTTTTTTAGGTGCTTCACCTAAGATGGGGATCTTTCTTAACGCTATGCGAAAATTACAACCGAAACTTTTAGTGATTACTGAACAGGAATCAAATCTGAATGGATGTAATTTAATGGAGAGAATCGATCGGGCATTGTATTTTTACGGTTCACTTTTTGACTGCTTGGAATCTACTGTTACGAGAACATCAGTCGAGAGACAAAAACTCGAGAGCATGCTTCTTGGAGAGCAAATAAAGAACATCATTACTTGTGAGGGAGTCGATAGAAAGGAAAGGCATGAGAAGCTGGAGCAATGGATTCAAAGACTTAAAATGGCTGGATTTGTGAAGGTACCTTTGAGTTACAATGGGAGGATAGAAGCGACAAACCTATTGCAGCGATATAGTCATAAATACAAGTTTAAAGAAGAAAATGATTGTTTACTTGTTTGTTGGAGTGACAGACCACTTTTTTCTGTATCGGCCTGGAAATTTAGGTGA
Protein sequence

>MsaT011791.1

MQLSCYALGSPYHWLRELRYDSHGSNPMIPLIECAKCVASGSIKNADIGLEYISQISSPHGNGVQRMVTYFSEALGYKIVKHLPGVYKALNSSKISSSSDDILVQKYFYDLCPFLKFSYLITNQAIIESMELEKVVHIIDLHCSEPAQWINLIQTLKKRPGGPPHLKITGIHEKKEVLEQMSFHLTTEAGILDFPLQFNPIISKLEDVDFENLPVKTGDAVAISSVLQLHSLLATDDEMVSSAGATSINMHRAAHMGQRTFAEWLERDMINAYILSPDSALSPLFLGASPKMGIFLNAMRKLQPKLLVITEQESNLNGCNLMERIDRALYFYGSLFDCLESTVTRTSVERQKLESMLLGEQIKNIITCEGVDRKERHEKLEQWIQRLKMAGFVKVPLSYNGRIEATNLLQRYSHKYKFKEENDCLLVCWSDRPLFSVSAWKFR*