AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011795

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011795 MtrunA17_Chr4g0047761 94.737 95 1 1 12 102 34 128 7.82e-58 181
MsaG011795 MtrunA17_Chr2g0282051 82.524 103 14 3 1 101 1 101 1.54e-53 168
MsaG011795 MtrunA17_Chr3g0127201 69.444 72 21 1 29 99 10 81 5.31e-32 114
MsaG011795 MtrunA17_Chr4g0047001 84.211 57 8 1 48 103 57 113 7.88e-31 112
MsaG011795 MtrunA17_Chr8g0374111 68.919 74 21 2 42 113 25 98 1.24e-29 106
MsaG011795 MtrunA17_Chr8g0374251 77.586 58 12 1 44 100 66 123 3.25e-29 107
MsaG011795 MtrunA17_Chr3g0126201 56.566 99 37 3 9 101 9 107 4.21e-29 107
MsaG011795 MtrunA17_Chr2g0328021 70.769 65 18 1 45 108 61 125 7.48e-29 105
MsaG011795 MtrunA17_Chr2g0326001 73.846 65 16 1 36 99 30 94 9.52e-29 105
MsaG011795 MtrunA17_Chr8g0346551 64.789 71 23 2 31 100 10 79 3.01e-28 103
MsaG011795 MtrunA17_Chr1g0188851 64.179 67 23 1 34 99 31 97 4.09e-28 103
MsaG011795 MtrunA17_Chr2g0282511 85.714 49 7 0 46 94 45 93 9.67e-28 103
MsaG011795 MtrunA17_Chr8g0367161 55.294 85 37 1 23 106 9 93 1.35e-27 103
MsaG011795 MtrunA17_Chr7g0223311 67.143 70 21 1 34 101 65 134 1.93e-27 103
MsaG011795 MtrunA17_Chr8g0341671 74.545 55 14 0 42 96 16 70 2.61e-27 100
MsaG011795 MtrunA17_Chr4g0030051 78.947 57 11 1 42 97 37 93 3.97e-27 101
MsaG011795 MtrunA17_Chr7g0237921 81.132 53 9 1 48 99 78 130 6.16e-27 101
MsaG011795 MtrunA17_Chr4g0010151 76.364 55 12 1 46 99 82 136 1.20e-26 101
MsaG011795 MtrunA17_Chr4g0061761 80.392 51 10 0 44 94 38 88 1.94e-26 99.8
MsaG011795 MtrunA17_Chr2g0308011 81.633 49 9 0 46 94 37 85 2.58e-26 97.4
MsaG011795 MtrunA17_Chr4g0031411 71.186 59 15 1 46 102 75 133 3.19e-26 99.8
MsaG011795 MtrunA17_Chr1g0177251 75.926 54 12 1 47 99 13 66 1.14e-25 97.4
MsaG011795 MtrunA17_Chr3g0117601 47.706 109 42 3 1 94 1 109 1.44e-25 97.8
MsaG011795 MtrunA17_Chr5g0411951 72.549 51 14 0 44 94 41 91 4.20e-25 96.7
MsaG011795 MtrunA17_Chr2g0282441 66.667 60 20 0 35 94 14 73 1.39e-24 95.1
MsaG011795 MtrunA17_Chr3g0092111 72.549 51 14 0 46 96 136 186 2.15e-24 95.9
MsaG011795 MtrunA17_Chr6g0460721 49.474 95 36 2 7 100 61 144 2.71e-24 94.4
MsaG011795 MtrunA17_Chr2g0283811 64.815 54 19 0 46 99 47 100 5.99e-24 89.7
MsaG011795 MtrunA17_Chr6g0460731 52.874 87 29 2 15 100 76 151 6.91e-24 94.0
MsaG011795 MtrunA17_Chr7g0217581 73.469 49 13 0 48 96 234 282 6.94e-24 94.7
MsaG011795 MtrunA17_Chr4g0042481 70.370 54 15 1 48 100 124 177 9.49e-24 94.4
MsaG011795 MtrunA17_Chr7g0254161 46.465 99 41 2 3 100 69 156 2.19e-23 92.8
MsaG011795 MtrunA17_Chr6g0453321 66.667 54 18 0 46 99 136 189 2.70e-23 93.2
MsaG011795 MtrunA17_Chr8g0355261 56.164 73 27 3 46 113 118 190 5.68e-23 92.0
MsaG011795 MtrunA17_Chr5g0417571 64.151 53 19 0 48 100 140 192 1.20e-22 89.7
MsaG011795 MtrunA17_Chr5g0417561 57.143 63 27 0 38 100 111 173 4.20e-22 89.0
MsaG011795 MtrunA17_Chr5g0417551 63.636 55 20 0 46 100 127 181 7.49e-22 88.2
MsaG011795 MtrunA17_Chr5g0417601 62.264 53 20 0 48 100 142 194 6.70e-21 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011795 AT1G28310.1 81.356 59 10 1 46 103 27 85 2.54e-30 110
MsaG011795 AT1G28310.2 81.356 59 10 1 46 103 51 109 3.87e-30 110
MsaG011795 AT1G51700.1 61.765 68 26 0 42 109 29 96 7.36e-29 103
MsaG011795 AT5G65590.1 51.579 95 35 2 28 111 13 107 8.17e-29 106
MsaG011795 AT3G50410.1 68.657 67 20 1 30 96 15 80 1.76e-28 104
MsaG011795 AT5G60200.1 80.000 55 10 1 46 99 55 109 2.67e-28 103
MsaG011795 AT3G55370.1 75.862 58 13 1 46 102 76 133 2.68e-28 105
MsaG011795 AT3G55370.2 77.193 57 12 1 46 101 76 132 2.86e-28 105
MsaG011795 AT3G55370.3 75.862 58 13 1 46 102 121 178 3.08e-28 105
MsaG011795 AT5G60850.1 64.557 79 24 2 35 109 37 115 4.00e-28 104
MsaG011795 AT5G66940.1 60.274 73 28 1 42 113 28 100 5.12e-28 102
MsaG011795 AT3G61850.2 62.857 70 25 1 35 103 51 120 8.91e-28 103
MsaG011795 AT3G61850.3 62.857 70 25 1 35 103 51 120 8.91e-28 103
MsaG011795 AT3G61850.1 62.857 70 25 1 35 103 63 132 1.24e-27 102
MsaG011795 AT3G61850.5 62.857 70 25 1 35 103 51 120 1.53e-27 103
MsaG011795 AT3G61850.4 62.857 70 25 1 35 103 63 132 1.87e-27 103
MsaG011795 AT3G21270.1 73.684 57 15 0 46 102 29 85 1.99e-27 100
MsaG011795 AT4G24060.1 66.154 65 21 1 36 99 43 107 1.99e-27 103
MsaG011795 AT3G45610.1 62.319 69 25 1 44 111 38 106 2.19e-27 101
MsaG011795 AT1G07640.1 70.968 62 16 1 43 102 18 79 3.16e-27 101
MsaG011795 AT2G28510.1 75.862 58 13 1 43 99 45 102 3.92e-27 101
MsaG011795 AT1G07640.2 70.968 62 16 1 43 102 74 135 7.21e-27 101
MsaG011795 AT1G07640.3 70.968 62 16 1 43 102 82 143 9.75e-27 101
MsaG011795 AT2G28810.2 68.333 60 19 0 35 94 66 125 2.05e-26 100
MsaG011795 AT2G28810.1 68.333 60 19 0 35 94 83 142 2.21e-26 100
MsaG011795 AT2G37590.1 71.930 57 15 1 46 101 89 145 4.16e-26 99.8
MsaG011795 AT1G21340.1 55.696 79 32 2 25 102 19 95 7.46e-26 97.4
MsaG011795 AT5G02460.1 76.364 55 12 1 46 99 95 149 7.50e-26 99.8
MsaG011795 AT5G62430.1 47.872 94 42 1 19 105 20 113 8.12e-26 98.2
MsaG011795 AT4G38000.1 71.698 53 15 0 46 98 41 93 1.61e-25 96.3
MsaG011795 AT4G00940.1 59.722 72 28 1 34 104 56 127 1.68e-25 97.4
MsaG011795 AT4G00940.3 59.722 72 28 1 34 104 56 127 1.68e-25 97.4
MsaG011795 AT4G00940.2 59.722 72 28 1 34 104 56 127 1.68e-25 97.4
MsaG011795 AT1G47655.1 73.077 52 14 0 43 94 26 77 2.33e-25 95.1
MsaG011795 AT1G64620.1 64.407 59 21 0 36 94 39 97 2.78e-25 97.8
MsaG011795 AT2G46590.1 76.000 50 12 0 44 93 66 115 2.80e-25 97.8
MsaG011795 AT2G46590.2 76.000 50 12 0 44 93 78 127 3.11e-25 97.8
MsaG011795 AT4G21050.1 57.971 69 28 1 48 115 26 94 5.27e-25 94.4
MsaG011795 AT3G52440.1 60.563 71 26 2 34 102 12 82 5.67e-25 95.1
MsaG011795 AT3G52440.2 60.563 71 26 2 34 102 31 101 9.36e-25 94.7
MsaG011795 AT3G47500.1 70.370 54 16 0 46 99 110 163 2.14e-24 96.3
MsaG011795 AT5G62940.1 78.723 47 10 0 48 94 75 121 7.30e-24 94.0
MsaG011795 AT5G39660.3 68.519 54 17 0 46 99 138 191 9.01e-24 94.7
MsaG011795 AT5G39660.1 68.519 54 17 0 46 99 138 191 9.01e-24 94.7
MsaG011795 AT5G39660.2 68.519 54 17 0 46 99 138 191 9.01e-24 94.7
MsaG011795 AT1G29160.1 64.815 54 19 0 46 99 62 115 1.07e-23 90.1
MsaG011795 AT1G69570.1 73.469 49 13 0 48 96 134 182 1.13e-23 94.0
MsaG011795 AT2G34140.1 64.815 54 19 0 46 99 58 111 1.15e-23 89.7
MsaG011795 AT1G26790.1 70.588 51 15 0 46 96 111 161 2.68e-23 92.4
MsaG011795 AT4G21040.1 61.667 60 21 1 48 105 27 86 8.51e-23 89.0
MsaG011795 AT4G21080.1 62.963 54 20 0 48 101 27 80 1.23e-21 86.3
MsaG011795 AT4G21030.1 60.870 46 18 0 48 93 23 68 1.30e-13 64.3

Find 19 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATTAGAATCTCAAGATT+TGG 0.267364 3:+12380080 MsaT011795.1:CDS
CAAATCTTGAGATTCTAATA+AGG 0.337572 3:-12380079 None:intergenic
CTCATTAGATGATGAGGCTT+TGG 0.369809 3:-12380108 None:intergenic
TTTCTAGCAGTGGACTTGAT+TGG 0.385715 3:+12380049 MsaT011795.1:CDS
TTCTTTCTGCCACCACCAAC+AGG 0.404107 3:-12380291 None:intergenic
ACAACAGGGCAAGAAGTAAC+TGG 0.437581 3:-12380339 None:intergenic
CATGGGTTTGAGTTCTAAGC+AGG 0.458863 3:+12380023 None:intergenic
GTGTTTGTTGAATCACATCT+TGG 0.501929 3:-12380168 None:intergenic
CTACGCAATGTTCCTGTTGG+TGG 0.512399 3:+12380279 MsaT011795.1:CDS
TGTAAAAGACACTGGACTAA+AGG 0.514243 3:+12380249 MsaT011795.1:CDS
CGCAATGTTCCTGTTGGTGG+TGG 0.518071 3:+12380282 MsaT011795.1:CDS
CAAGAAGTAACTGGTGTGGT+TGG 0.534676 3:-12380330 None:intergenic
AAGCAGGTTATTTCTAGCAG+TGG 0.536340 3:+12380039 MsaT011795.1:CDS
GTAGAGCTTGTAAAAGACAC+TGG 0.584007 3:+12380241 MsaT011795.1:CDS
AAAAGACACTGGACTAAAGG+TGG 0.598362 3:+12380252 MsaT011795.1:CDS
ACTCTACGCAATGTTCCTGT+TGG 0.603022 3:+12380276 MsaT011795.1:CDS
GTGGTGGCAGAAAGAATAAG+AGG 0.637344 3:+12380298 MsaT011795.1:CDS
CAAGCTCTACAGAAATGACG+AGG 0.668993 3:-12380228 None:intergenic
AGGGCAAGAAGTAACTGGTG+TGG 0.692908 3:-12380334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 12380024 12380371 12380024 ID=MsaG011795
Chr3 mRNA 12380024 12380371 12380024 ID=MsaT011795.1;Parent=MsaG011795
Chr3 exon 12380024 12380371 12380024 ID=MsaT011795.1.exon1;Parent=MsaT011795.1
Chr3 CDS 12380024 12380371 12380024 ID=cds.MsaT011795.1;Parent=MsaT011795.1
Gene Sequence

>MsaT011795.1

ATGGGTTTGAGTTCTAAGCAGGTTATTTCTAGCAGTGGACTTGATTGGAAACAAACCTTATTAGAATCTCAAGATTTGGAACTTCCAAAGCCTCATCATCTAATGAGAAAACAACAACAGCAACAAGCAGAACATTTGAACTGTCCAAGATGTGATTCAACAAACACAAAGTTTTGTTACTACAATAATTACAACAAGTCTCAACCTCGTCATTTCTGTAGAGCTTGTAAAAGACACTGGACTAAAGGTGGAACTCTACGCAATGTTCCTGTTGGTGGTGGCAGAAAGAATAAGAGGATCAAGAAACCAACCACACCAGTTACTTCTTGCCCTGTTGTAAAGCATTGA
Protein sequence

>MsaT011795.1

MGLSSKQVISSSGLDWKQTLLESQDLELPKPHHLMRKQQQQQAEHLNCPRCDSTNTKFCYYNNYNKSQPRHFCRACKRHWTKGGTLRNVPVGGGRKNKRIKKPTTPVTSCPVVKH*