AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011930 MtrunA17_Chr7g0224601 62.706 303 109 2 12 310 2 304 1.93e-121 351
MsaG011930 MtrunA17_Chr8g0392781 53.371 356 131 2 1 321 1 356 1.15e-118 346
MsaG011930 MtrunA17_Chr1g0177151 61.479 257 95 1 1 257 1 253 5.42e-113 328
MsaG011930 MtrunA17_Chr3g0099561 59.857 279 112 0 38 316 26 304 2.19e-111 325
MsaG011930 MtrunA17_Chr5g0444621 63.333 210 77 0 70 279 52 261 5.73e-88 265
MsaG011930 MtrunA17_Chr6g0477171 65.306 196 68 0 62 257 48 243 7.93e-85 256
MsaG011930 MtrunA17_Chr3g0083811 98.276 116 2 0 186 301 1 116 5.24e-78 233
MsaG011930 MtrunA17_Chr3g0083821 72.973 111 30 0 54 164 10 120 3.98e-51 165
MsaG011930 MtrunA17_Chr5g0444631 67.925 53 17 0 112 164 4 56 2.50e-19 80.5
MsaG011930 MtrunA17_Chr6g0477181 61.017 59 23 0 1 59 1 59 6.07e-15 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011930 AT3G20800.1 55.814 301 127 2 21 316 17 316 5.24e-110 323
MsaG011930 AT5G12980.1 53.455 275 127 1 31 304 28 302 7.87e-99 294
MsaG011930 AT2G32550.2 35.510 245 142 5 53 285 67 307 4.75e-35 130
MsaG011930 AT2G32550.3 35.897 234 134 5 64 285 39 268 8.44e-35 128
MsaG011930 AT2G32550.1 35.648 216 123 5 82 285 2 213 5.73e-30 114

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGCTTATCTCAAAATTAC+AGG 0.218940 3:+15240180 MsaT011930.1:CDS
AGGAACTTGGACCTTTATTA+TGG 0.221177 3:+15237253 MsaT011930.1:CDS
AGAAATGAGATCCTTGTATC+AGG 0.301701 3:-15238263 None:intergenic
ATAGAAACCTATGACTTCTT+TGG 0.317203 3:-15239817 None:intergenic
AGGAATGGATATAAATATAA+AGG 0.360461 3:-15238653 None:intergenic
CAATGTTCTTGCACTACTTC+AGG 0.367374 3:+15238033 MsaT011930.1:CDS
GTTGCTAGTCTTGGAGTCAT+TGG 0.372727 3:+15238716 MsaT011930.1:CDS
TATATTAGACGATGATGGTT+TGG 0.376298 3:+15240037 MsaT011930.1:CDS
TTGGCATCTTTGAGCCTGTT+TGG 0.379455 3:-15240322 None:intergenic
CCAACAGTATTCCATAATAA+AGG 0.383147 3:-15237264 None:intergenic
TATGATCAGTGGTGTTAGAT+TGG 0.407147 3:-15236105 None:intergenic
GTTAACACCAAAGAAGTCAT+AGG 0.420089 3:+15239810 MsaT011930.1:CDS
TGTTGTTACATGAGAATGTT+GGG 0.422120 3:+15240810 MsaT011930.1:CDS
TCAGAGCACCAAGTTATTTG+AGG 0.428345 3:+15237233 MsaT011930.1:intron
TAACAACTTATCTTCCAAAC+AGG 0.438040 3:+15240308 MsaT011930.1:CDS
TTCTTTGTTGAGAGCGATTG+AGG 0.439462 3:-15236046 None:intergenic
GATTGTGAGGGAGAGAGATT+CGG 0.440290 3:-15237998 None:intergenic
TAACACAGTTTGAGAATTTG+AGG 0.440707 3:+15238693 MsaT011930.1:CDS
AATCTAACACCACTGATCAT+AGG 0.444777 3:+15236107 MsaT011930.1:CDS
AATTTGAGGGTTGCTAGTCT+TGG 0.451958 3:+15238707 MsaT011930.1:CDS
GGAGGAATTGATGATATTGT+AGG 0.453082 3:+15240863 MsaT011930.1:CDS
AAAATTATATTAGACGATGA+TGG 0.467412 3:+15240032 MsaT011930.1:CDS
CACCCTCACCTCGTTTGTTG+AGG 0.472935 3:+15240138 MsaT011930.1:CDS
TGTTATTGAACTCAGCAATC+CGG 0.481393 3:+15236150 MsaT011930.1:CDS
ATGATCAGTGGTGTTAGATT+GGG 0.483339 3:-15236104 None:intergenic
ACCAAGTTATTTGAGGAACT+TGG 0.485648 3:+15237240 MsaT011930.1:CDS
AGATTCGGTGGTGAAAGAGT+AGG 0.488495 3:-15237983 None:intergenic
AACCGAGTTCCTTTAGAAGC+AGG 0.491153 3:+15240836 MsaT011930.1:CDS
GCTAGTCTTGGAGTCATTGG+TGG 0.491588 3:+15238719 MsaT011930.1:CDS
CCTTTATTATGGAATACTGT+TGG 0.492405 3:+15237264 MsaT011930.1:CDS
CCTCCTCTACCTGCTTCTAA+AGG 0.503277 3:-15240845 None:intergenic
GTTCCTTTAGAAGCAGGTAG+AGG 0.520910 3:+15240842 MsaT011930.1:CDS
TCCAAGTTCCTCAAATAACT+TGG 0.522228 3:-15237241 None:intergenic
TGGCATCTCACCCTGATACA+AGG 0.537570 3:+15238252 MsaT011930.1:CDS
AACACAGTTTGAGAATTTGA+GGG 0.539961 3:+15238694 MsaT011930.1:CDS
CTGTTGTTACATGAGAATGT+TGG 0.540471 3:+15240809 MsaT011930.1:CDS
AGTCATTGGTGGCTCGATGA+AGG 0.546457 3:+15238730 MsaT011930.1:CDS
GAGGAATTGATGATATTGTA+GGG 0.550926 3:+15240864 MsaT011930.1:CDS
CCTTTAGAAGCAGGTAGAGG+AGG 0.553169 3:+15240845 MsaT011930.1:CDS
AGCCTCAACAAACGAGGTGA+GGG 0.559488 3:-15240140 None:intergenic
TGTTTGTCTAATATGCAAAA+CGG 0.565201 3:+15239864 MsaT011930.1:CDS
ACAAGGATCTCATTTCTCAA+TGG 0.566776 3:+15238269 MsaT011930.1:CDS
AAGCCTCAACAAACGAGGTG+AGG 0.569309 3:-15240141 None:intergenic
TGCTCTCTATGTGCTCTCCA+AGG 0.584461 3:+15236186 MsaT011930.1:CDS
GAAATGAGATCCTTGTATCA+GGG 0.596244 3:-15238262 None:intergenic
CACACTCGAGTTGATTGTGA+GGG 0.603583 3:-15238010 None:intergenic
TAACACCACTGATCATAGGA+TGG 0.625981 3:+15236111 MsaT011930.1:CDS
CTCAACAAACGAGGTGAGGG+TGG 0.629401 3:-15240137 None:intergenic
TACCTGCTTCTAAAGGAACT+CGG 0.631809 3:-15240838 None:intergenic
GATTGCTGAGTTCAATAACA+AGG 0.649486 3:-15236147 None:intergenic
TTAGATTGGGTAGAATCACC+AGG 0.651677 3:-15236091 None:intergenic
ATCAAAAGCCTCAACAAACG+AGG 0.660997 3:-15240146 None:intergenic
GCACACTCGAGTTGATTGTG+AGG 0.665692 3:-15238011 None:intergenic
GTAAGCCATCCTATGATCAG+TGG 0.669763 3:-15236116 None:intergenic
TGTGAGGGAGAGAGATTCGG+TGG 0.689243 3:-15237995 None:intergenic
GTTGTTACATGAGAATGTTG+GGG 0.746517 3:+15240811 MsaT011930.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 15236031 15240907 15236031 ID=MsaG011930
Chr3 mRNA 15236031 15240907 15236031 ID=MsaT011930.1;Parent=MsaG011930
Chr3 exon 15236031 15236207 15236031 ID=MsaT011930.1.exon1;Parent=MsaT011930.1
Chr3 CDS 15236031 15236207 15236031 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15237237 15237308 15237237 ID=MsaT011930.1.exon2;Parent=MsaT011930.1
Chr3 CDS 15237237 15237308 15237237 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15237965 15238054 15237965 ID=MsaT011930.1.exon3;Parent=MsaT011930.1
Chr3 CDS 15237965 15238054 15237965 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15238248 15238290 15238248 ID=MsaT011930.1.exon4;Parent=MsaT011930.1
Chr3 CDS 15238248 15238290 15238248 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15238645 15238751 15238645 ID=MsaT011930.1.exon5;Parent=MsaT011930.1
Chr3 CDS 15238645 15238751 15238645 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15239810 15239905 15239810 ID=MsaT011930.1.exon6;Parent=MsaT011930.1
Chr3 CDS 15239810 15239905 15239810 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15240011 15240201 15240011 ID=MsaT011930.1.exon7;Parent=MsaT011930.1
Chr3 CDS 15240011 15240201 15240011 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15240294 15240363 15240294 ID=MsaT011930.1.exon8;Parent=MsaT011930.1
Chr3 CDS 15240294 15240363 15240294 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Chr3 exon 15240782 15240907 15240782 ID=MsaT011930.1.exon9;Parent=MsaT011930.1
Chr3 CDS 15240782 15240907 15240782 ID=cds.MsaT011930.1;Parent=MsaT011930.1
Gene Sequence

>MsaT011930.1

ATGGCACACTTTACTCCTCAATCGCTCTCAACAAAGAACAATGCATCGTTTATAGCTTTTCCTGGTGATTCTACCCAATCTAACACCACTGATCATAGGATGGCTTACATGCAAAGCCTTGTTATTGAACTCAGCAATCCGGATCTTAGAGAAAATGCTCTCTATGTGCTCTCCAAGAGCACCAAGTTATTTGAGGAACTTGGACCTTTATTATGGAATACTGTTGGTACTATTGCAGTATTTTTACAGGAAATACTTTCAATATATCCTACTCTTTCACCACCGAATCTCTCTCCCTCACAATCAACTCGAGTGTGCAATGTTCTTGCACTACTTCAGTGTGTGGCATCTCACCCTGATACAAGGATCTCATTTCTCAATGCTAATATGCCTTTATATTTATATCCATTCCTTAAAACAACAAGCAAGTTAACACAGTTTGAGAATTTGAGGGTTGCTAGTCTTGGAGTCATTGGTGGCTCGATGAAGGTTAACACCAAAGAAGTCATAGGTTTCTATCTCTCAAGTGAGATAATTTCACTATGTTTGTCTAATATGCAAAACGGCAATGAATTGACAAAAATTGTTGCAACATTTATAATTCTGAAAATTATATTAGACGATGATGGTTTGGCTCGTGTTTGTGCTACAATAGAGAATTTTTTTGGAGTATGTCGAGTTTTGAACATGATGTTGGAAGATCTTGAGAATCCACCCTCACCTCGTTTGTTGAGGCTTTTGATTTCATGTTATTCTCGCTTATCTCAAAATTACAGGGCTAGAGTTGCATTAACAACTTATCTTCCAAACAGGCTCAAAGATGCCAATCTCATCAATTTTTTTCGTGATGATCCAAAAACTATGAGGTTGGTACTGTTGTTACATGAGAATGTTGGGGTGAACCGAGTTCCTTTAGAAGCAGGTAGAGGAGGAATTGATGATATTGTAGGGCCATCATTATCAAGAAAATGA

Protein sequence

>MsaT011930.1

MAHFTPQSLSTKNNASFIAFPGDSTQSNTTDHRMAYMQSLVIELSNPDLRENALYVLSKSTKLFEELGPLLWNTVGTIAVFLQEILSIYPTLSPPNLSPSQSTRVCNVLALLQCVASHPDTRISFLNANMPLYLYPFLKTTSKLTQFENLRVASLGVIGGSMKVNTKEVIGFYLSSEIISLCLSNMQNGNELTKIVATFIILKIILDDDGLARVCATIENFFGVCRVLNMMLEDLENPPSPRLLRLLISCYSRLSQNYRARVALTTYLPNRLKDANLINFFRDDPKTMRLVLLLHENVGVNRVPLEAGRGGIDDIVGPSLSRK*