AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011932


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011932 MtrunA17_Chr7g0224601 62.376 303 110 2 12 310 2 304 1.72e-119 346
MsaG011932 MtrunA17_Chr8g0392781 53.090 356 132 2 1 321 1 356 1.13e-117 343
MsaG011932 MtrunA17_Chr1g0177151 61.479 257 95 1 1 257 1 253 3.17e-113 328
MsaG011932 MtrunA17_Chr3g0099561 60.215 279 111 0 38 316 26 304 4.55e-113 330
MsaG011932 MtrunA17_Chr5g0444621 62.857 210 78 0 70 279 52 261 9.08e-87 261
MsaG011932 MtrunA17_Chr6g0477171 63.819 199 72 0 59 257 45 243 3.60e-84 254
MsaG011932 MtrunA17_Chr3g0083811 97.414 116 3 0 186 301 1 116 7.65e-77 231
MsaG011932 MtrunA17_Chr3g0083821 73.874 111 29 0 54 164 10 120 8.66e-51 164
MsaG011932 MtrunA17_Chr5g0444631 66.038 53 18 0 112 164 4 56 1.96e-18 78.2
MsaG011932 MtrunA17_Chr6g0477181 61.017 59 23 0 1 59 1 59 2.60e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011932 AT3G20800.1 56.146 301 126 2 21 316 17 316 7.46e-112 327
MsaG011932 AT5G12980.1 54.182 275 125 1 31 304 28 302 1.53e-100 298
MsaG011932 AT2G32550.2 35.510 245 142 5 53 285 67 307 6.56e-35 129
MsaG011932 AT2G32550.3 35.897 234 134 5 64 285 39 268 1.26e-34 128
MsaG011932 AT2G32550.1 35.185 216 124 5 82 285 2 213 9.97e-30 113

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAACTTGGACCTTTATTA+TGG 0.221177 3:+15261642 MsaT011932.1:CDS
CTCGCTTATCTCAAAATTGC+AGG 0.298861 3:+15264197 MsaT011932.1:CDS
AGAAATGAGATCCTTGTATC+AGG 0.301701 3:-15262656 None:intergenic
ATGGAATTCTGTTGGTACTA+TGG 0.311879 3:+15261661 MsaT011932.1:CDS
ATAGAAACCTATGACTTCTT+TGG 0.317203 3:-15263834 None:intergenic
CCAACAGAATTCCATAATAA+AGG 0.356692 3:-15261653 None:intergenic
CAATGTTCTTGCATTACTTC+AGG 0.357841 3:+15262425 MsaT011932.1:CDS
AGGAATGGATATAAATATAA+AGG 0.360461 3:-15263046 None:intergenic
GTTGCTAGTCTTGGAGTCAT+TGG 0.372727 3:+15263109 MsaT011932.1:CDS
TATATTAGACGATGATGGTT+TGG 0.376298 3:+15264054 MsaT011932.1:CDS
TTGGCATCTTTGAGCCTGTT+TGG 0.379455 3:-15264341 None:intergenic
TATGATCAGTGGTGCTAGAT+TGG 0.401740 3:-15260092 None:intergenic
GTTAACACCAAAGAAGTCAT+AGG 0.420089 3:+15263827 MsaT011932.1:CDS
TGTTGTTACATGAGAATGTT+GGG 0.422120 3:+15264829 MsaT011932.1:CDS
TCAGAGCACCAAGTTATTTG+AGG 0.428345 3:+15261622 MsaT011932.1:intron
TAACAACTTATCTTCCAAAC+AGG 0.438040 3:+15264327 MsaT011932.1:CDS
TTCTTTGTTGAGAGCGATTG+AGG 0.439462 3:-15260033 None:intergenic
GATTGTGAGGGAGAGAGATT+CGG 0.440290 3:-15262390 None:intergenic
AATTTGAGGGTTGCTAGTCT+TGG 0.451958 3:+15263100 MsaT011932.1:CDS
GGAGGAATTGATGATATTGT+AGG 0.453082 3:+15264882 MsaT011932.1:CDS
ATGATCAGTGGTGCTAGATT+GGG 0.463771 3:-15260091 None:intergenic
AATCTAGCACCACTGATCAT+AGG 0.464495 3:+15260094 MsaT011932.1:CDS
AAAATTATATTAGACGATGA+TGG 0.467412 3:+15264049 MsaT011932.1:CDS
CACCCTCACCTCGTTTGTTG+AGG 0.472935 3:+15264155 MsaT011932.1:CDS
TAACACCCTTTGAGAATTTG+AGG 0.473351 3:+15263086 MsaT011932.1:CDS
TGTTATTGAACTCAGCAATC+CGG 0.481393 3:+15260137 MsaT011932.1:CDS
ACCAAGTTATTTGAGGAACT+TGG 0.485648 3:+15261629 MsaT011932.1:CDS
AGATTCGGTGGTGAAAGAGT+AGG 0.488495 3:-15262375 None:intergenic
CCTTTATTATGGAATTCTGT+TGG 0.489673 3:+15261653 MsaT011932.1:CDS
AACCGAGTTCCTTTAGAAGC+AGG 0.491153 3:+15264855 MsaT011932.1:CDS
GCTAGTCTTGGAGTCATTGG+TGG 0.491588 3:+15263112 MsaT011932.1:CDS
CCTCCTCTACCTGCTTCTAA+AGG 0.503277 3:-15264864 None:intergenic
GTTCCTTTAGAAGCAGGTAG+AGG 0.520910 3:+15264861 MsaT011932.1:CDS
TCCAAGTTCCTCAAATAACT+TGG 0.522228 3:-15261630 None:intergenic
AACACCCTTTGAGAATTTGA+GGG 0.530151 3:+15263087 MsaT011932.1:CDS
TGGCATCTCACCCTGATACA+AGG 0.537570 3:+15262645 MsaT011932.1:CDS
CTGTTGTTACATGAGAATGT+TGG 0.540471 3:+15264828 MsaT011932.1:CDS
AGTCATTGGTGGCTCGATGA+AGG 0.546340 3:+15263123 MsaT011932.1:CDS
GCAACCCTCAAATTCTCAAA+GGG 0.548073 3:-15263091 None:intergenic
GAGGAATTGATGATATTGTA+GGG 0.550926 3:+15264883 MsaT011932.1:CDS
CCTTTAGAAGCAGGTAGAGG+AGG 0.553169 3:+15264864 MsaT011932.1:CDS
AGCCTCAACAAACGAGGTGA+GGG 0.559488 3:-15264157 None:intergenic
AGCAACCCTCAAATTCTCAA+AGG 0.559876 3:-15263092 None:intergenic
TGTTTGTCTAATATGCAAAA+CGG 0.565201 3:+15263881 MsaT011932.1:CDS
ACAAGGATCTCATTTCTCAA+TGG 0.566776 3:+15262662 MsaT011932.1:CDS
AAGCCTCAACAAACGAGGTG+AGG 0.569309 3:-15264158 None:intergenic
TGCTCTCTATGTGCTCTCCA+AGG 0.584461 3:+15260173 MsaT011932.1:CDS
CTAGATTGGGTAGAATCACC+AGG 0.589884 3:-15260078 None:intergenic
GAAATGAGATCCTTGTATCA+GGG 0.596244 3:-15262655 None:intergenic
CACACTCGAGTTGATTGTGA+GGG 0.603583 3:-15262402 None:intergenic
TAGCACCACTGATCATAGGA+TGG 0.627844 3:+15260098 MsaT011932.1:CDS
CTCAACAAACGAGGTGAGGG+TGG 0.629401 3:-15264154 None:intergenic
TACCTGCTTCTAAAGGAACT+CGG 0.631809 3:-15264857 None:intergenic
GATTGCTGAGTTCAATAACA+AGG 0.649486 3:-15260134 None:intergenic
ATCAAAAGCCTCAACAAACG+AGG 0.660997 3:-15264163 None:intergenic
GCACACTCGAGTTGATTGTG+AGG 0.665692 3:-15262403 None:intergenic
GTAAGCCATCCTATGATCAG+TGG 0.669763 3:-15260103 None:intergenic
TGTGAGGGAGAGAGATTCGG+TGG 0.689243 3:-15262387 None:intergenic
GTTGTTACATGAGAATGTTG+GGG 0.746517 3:+15264830 MsaT011932.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 15260018 15264926 15260018 ID=MsaG011932
Chr3 mRNA 15260018 15264926 15260018 ID=MsaT011932.1;Parent=MsaG011932
Chr3 exon 15260018 15260194 15260018 ID=MsaT011932.1.exon1;Parent=MsaT011932.1
Chr3 CDS 15260018 15260194 15260018 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15261626 15261697 15261626 ID=MsaT011932.1.exon2;Parent=MsaT011932.1
Chr3 CDS 15261626 15261697 15261626 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15262357 15262446 15262357 ID=MsaT011932.1.exon3;Parent=MsaT011932.1
Chr3 CDS 15262357 15262446 15262357 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15262641 15262683 15262641 ID=MsaT011932.1.exon4;Parent=MsaT011932.1
Chr3 CDS 15262641 15262683 15262641 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15263038 15263144 15263038 ID=MsaT011932.1.exon5;Parent=MsaT011932.1
Chr3 CDS 15263038 15263144 15263038 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15263827 15263922 15263827 ID=MsaT011932.1.exon6;Parent=MsaT011932.1
Chr3 CDS 15263827 15263922 15263827 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15264028 15264218 15264028 ID=MsaT011932.1.exon7;Parent=MsaT011932.1
Chr3 CDS 15264028 15264218 15264028 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15264313 15264382 15264313 ID=MsaT011932.1.exon8;Parent=MsaT011932.1
Chr3 CDS 15264313 15264382 15264313 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Chr3 exon 15264801 15264926 15264801 ID=MsaT011932.1.exon9;Parent=MsaT011932.1
Chr3 CDS 15264801 15264926 15264801 ID=cds.MsaT011932.1;Parent=MsaT011932.1
Gene Sequence

>MsaT011932.1

ATGGCACACTTTACTCCTCAATCGCTCTCAACAAAGAACGATACATCGTTTATAGCTTTTCCTGGTGATTCTACCCAATCTAGCACCACTGATCATAGGATGGCTTACATGCAAAGCCTTGTTATTGAACTCAGCAATCCGGATCTTAGAGAAAATGCTCTCTATGTGCTCTCCAAGAGCACCAAGTTATTTGAGGAACTTGGACCTTTATTATGGAATTCTGTTGGTACTATGGCAGTATTTTTACAGGAAATAATTTCAATATATCCTACTCTTTCACCACCGAATCTCTCTCCCTCACAATCAACTCGAGTGTGCAATGTTCTTGCATTACTTCAGTGTGTGGCATCTCACCCTGATACAAGGATCTCATTTCTCAATGCTAATATGCCTTTATATTTATATCCATTCCTTAAAACGACAAGCAAGTTAACACCCTTTGAGAATTTGAGGGTTGCTAGTCTTGGAGTCATTGGTGGCTCGATGAAGGTTAACACCAAAGAAGTCATAGGTTTCTATCTCTCAAGTGAGATAATTTCACTATGTTTGTCTAATATGCAAAACGGCAATGAATTGACAAAAATTGTTGCAACATTTATAATTCTGAAAATTATATTAGACGATGATGGTTTGGCTCGTGTTTGTGCTACAACAGAGAATTTTTTTGGAGTATGTCGAGTTTTGAACATGATGTTGGAAGATCTTGAGAATCCACCCTCACCTCGTTTGTTGAGGCTTTTGATTTCATGTTATTCTCGCTTATCTCAAAATTGCAGGGCTAAAGTTGCATTAACAACTTATCTTCCAAACAGGCTCAAAGATGCCAATCTCATCAATTTTTTTCGTGATGATCCAAAAACTATGAGGTTGGTACTGTTGTTACATGAGAATGTTGGGGTGAACCGAGTTCCTTTAGAAGCAGGTAGAGGAGGAATTGATGATATTGTAGGGCCATCATTATCAAGAAAATGA

Protein sequence

>MsaT011932.1

MAHFTPQSLSTKNDTSFIAFPGDSTQSSTTDHRMAYMQSLVIELSNPDLRENALYVLSKSTKLFEELGPLLWNSVGTMAVFLQEIISIYPTLSPPNLSPSQSTRVCNVLALLQCVASHPDTRISFLNANMPLYLYPFLKTTSKLTPFENLRVASLGVIGGSMKVNTKEVIGFYLSSEIISLCLSNMQNGNELTKIVATFIILKIILDDDGLARVCATTENFFGVCRVLNMMLEDLENPPSPRLLRLLISCYSRLSQNCRAKVALTTYLPNRLKDANLINFFRDDPKTMRLVLLLHENVGVNRVPLEAGRGGIDDIVGPSLSRK*