AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011970 MtrunA17_Chr3g0083491 94.361 1330 56 4 2 1313 3 1331 0.0 2548
MsaG011970 MtrunA17_Chr2g0320751 26.316 608 314 23 712 1276 476 992 2.21e-38 156
MsaG011970 MtrunA17_Chr1g0168031 23.394 607 340 19 711 1266 588 1120 1.06e-25 115
MsaG011970 MtrunA17_Chr2g0281601 22.292 637 391 20 712 1271 661 1270 8.31e-23 106
MsaG011970 MtrunA17_Chr7g0255821 30.568 229 138 7 712 933 294 508 7.70e-21 99.4
MsaG011970 MtrunA17_Chr7g0255821 27.815 151 105 2 1152 1299 668 817 1.46e-13 75.9
MsaG011970 MtrunA17_Chr4g0024921 22.430 535 292 17 779 1269 447 902 4.11e-20 97.1
MsaG011970 MtrunA17_Chr1g0212331 28.488 172 112 4 1130 1300 871 1032 3.63e-16 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011970 AT3G54460.1 53.038 1399 514 21 11 1313 5 1356 0.0 1410
MsaG011970 AT3G54460.2 56.438 1095 396 12 258 1313 7 1059 0.0 1213
MsaG011970 AT5G22750.1 25.574 610 312 20 714 1276 491 1005 9.56e-41 164
MsaG011970 AT1G11100.5 25.596 629 396 24 714 1308 491 1081 1.12e-33 141
MsaG011970 AT1G11100.1 25.596 629 396 24 714 1308 634 1224 1.53e-33 141
MsaG011970 AT1G11100.3 24.368 673 393 24 714 1308 491 1125 1.47e-30 131
MsaG011970 AT1G11100.4 24.368 673 393 24 714 1308 634 1268 1.82e-30 131
MsaG011970 AT1G11100.2 24.368 673 393 24 714 1308 633 1267 2.23e-30 131
MsaG011970 AT1G61140.3 22.831 657 390 21 714 1302 508 1115 1.64e-25 115
MsaG011970 AT1G61140.1 22.831 657 390 21 714 1302 666 1273 1.86e-25 115
MsaG011970 AT3G20010.2 22.624 663 399 21 714 1300 124 748 2.20e-25 114
MsaG011970 AT1G50410.2 25.000 520 331 19 782 1275 466 952 4.60e-23 107
MsaG011970 AT1G50410.1 25.000 520 331 19 782 1275 466 952 4.60e-23 107
MsaG011970 AT1G50410.3 25.000 520 331 19 782 1275 466 952 4.60e-23 107
MsaG011970 AT5G05130.1 29.545 220 137 8 705 918 349 556 3.18e-19 94.7
MsaG011970 AT5G43530.1 24.425 348 206 11 714 1029 742 1064 1.62e-17 89.4
MsaG011970 AT5G43530.1 30.657 137 94 1 1131 1266 1108 1244 6.80e-14 77.4
MsaG011970 AT1G02670.3 32.479 117 78 1 1152 1267 539 655 3.47e-15 81.3
MsaG011970 AT1G02670.6 32.479 117 78 1 1152 1267 512 628 3.54e-15 81.3
MsaG011970 AT1G02670.7 32.479 117 78 1 1152 1267 516 632 5.91e-15 80.5
MsaG011970 AT1G02670.1 32.479 117 78 1 1152 1267 527 643 6.32e-15 80.5
MsaG011970 AT1G05120.2 31.092 119 81 1 1152 1269 725 843 1.27e-14 79.7
MsaG011970 AT1G05120.1 31.092 119 81 1 1152 1269 682 800 1.27e-14 79.7
MsaG011970 AT3G20010.3 24.150 294 200 8 1015 1300 495 773 2.32e-14 78.6
MsaG011970 AT3G20010.1 24.138 290 197 8 1015 1296 761 1035 3.78e-14 78.2
MsaG011970 AT3G20010.5 24.138 290 197 8 1015 1296 761 1035 3.78e-14 78.2
MsaG011970 AT3G20010.7 24.138 290 197 8 1015 1296 761 1035 3.78e-14 78.2
MsaG011970 AT1G02670.5 31.068 103 70 1 1152 1253 512 614 7.13e-12 70.5
MsaG011970 AT1G02670.2 31.068 103 70 1 1152 1253 539 641 7.46e-12 70.5

Find 291 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTTGTCAATAGAAATTT+TGG 0.175597 3:-15857880 None:intergenic
CCGAAATCTGGTTCTGTTGC+TGG 0.192784 3:+15854629 MsaT011970.1:CDS
CTCCGAAATTTCCACTGTTT+TGG 0.214009 3:-15858827 None:intergenic
TGTTGAATGAACAGGATAAT+TGG 0.223532 3:+15859430 MsaT011970.1:intron
CTGATCAGGATTTAACAATT+TGG 0.226507 3:-15857250 None:intergenic
CATTGCGTTGAATCGATAAT+TGG 0.231827 3:-15854392 None:intergenic
GGTAGTTGTTCCAGCAAATT+TGG 0.245133 3:+15858148 MsaT011970.1:CDS
TGATGTTTATCTTCCGGTTT+TGG 0.272070 3:+15854583 MsaT011970.1:CDS
TGGAGGTTGATTCTAGGGTT+AGG 0.272143 3:+15854546 MsaT011970.1:CDS
TAATGCAAGTGCACTGGTTT+AGG 0.287123 3:+15858345 MsaT011970.1:CDS
CATCTGCGCACTCGCTTGCC+TGG 0.292942 3:+15858252 MsaT011970.1:CDS
CAGCAAATTTGGTTGATCAT+TGG 0.295875 3:+15858159 MsaT011970.1:CDS
CCAGAGGTTGAAAGCTTTGC+AGG 0.299028 3:+15859500 MsaT011970.1:CDS
CAATGATCAACCAAATTTGC+TGG 0.299556 3:-15858158 None:intergenic
ATACTGGCGGATCTGGGATT+TGG 0.301244 3:+15856663 MsaT011970.1:CDS
GTTGATACTTCATATGGTTC+TGG 0.303091 3:+15857513 MsaT011970.1:CDS
GTGTTGTGGAGGTTGATTCT+AGG 0.304442 3:+15854540 MsaT011970.1:CDS
TTCCTTCATGAAGAAGCTTA+TGG 0.308034 3:+15858530 MsaT011970.1:CDS
CACAAAGTATTTCAAGCATT+TGG 0.310911 3:+15857981 MsaT011970.1:CDS
TCTGGCCGTGCCATTGAATC+AGG 0.311287 3:-15859259 None:intergenic
TGGGCCCACTGGACCTCAAT+AGG 0.312568 3:+15856726 MsaT011970.1:CDS
AATGGTCTAGATTCTACTTC+AGG 0.317795 3:+15858953 MsaT011970.1:CDS
TATTGACAAGCTTGCAGAAA+TGG 0.319995 3:+15857890 MsaT011970.1:CDS
AGGCTTTCATGCTGTGTTGC+TGG 0.327727 3:+15858869 MsaT011970.1:CDS
CTAGATGTTGATTGCCAATC+AGG 0.333695 3:-15859456 None:intergenic
ATGGACAATACTGGCGGATC+TGG 0.335524 3:+15856656 MsaT011970.1:CDS
AAAGTGCAGTTGTTTAAAAT+AGG 0.342173 3:-15857933 None:intergenic
ATCCAAAACAGTGGAAATTT+CGG 0.343593 3:+15858825 MsaT011970.1:CDS
TTGGAGAAAATGAGAGCATT+TGG 0.344489 3:+15856477 MsaT011970.1:CDS
TACTGGCGGATCTGGGATTT+GGG 0.346850 3:+15856664 MsaT011970.1:CDS
GTCAATTGCATGTTTATGTT+TGG 0.351216 3:+15858213 MsaT011970.1:CDS
CTTTCTGATTGTCATGTACT+TGG 0.351744 3:+15856503 MsaT011970.1:CDS
GGAGGTTGATTCTAGGGTTA+GGG 0.353484 3:+15854547 MsaT011970.1:CDS
GGGTGGCAGTTTCCGAAATC+TGG 0.356692 3:+15854617 MsaT011970.1:CDS
TTCCATGTAAATACTGTAAC+TGG 0.361846 3:+15856926 MsaT011970.1:CDS
GTTAATGGTATCAAATATAC+TGG 0.364522 3:+15859834 MsaT011970.1:CDS
TCTCTGTGAGCCATTAGATT+TGG 0.364555 3:+15858097 MsaT011970.1:CDS
AAAGCTTGCGGATAACAGTA+TGG 0.366539 3:+15857626 MsaT011970.1:CDS
TAATGCTACTTGAACATTCC+TGG 0.371689 3:-15859620 None:intergenic
TGCTGAAAGCAATTATCTAC+TGG 0.373523 3:+15860808 MsaT011970.1:CDS
GTTGATAGAGCTGCATATAA+AGG 0.376494 3:-15856887 None:intergenic
GCGAAGAAGGTGAAGAAGGT+TGG 0.378619 3:+15854431 MsaT011970.1:CDS
GTTGCAAATGACTATTTCAT+TGG 0.380919 3:+15858421 MsaT011970.1:CDS
CTTGGGGTACAGACCACTTT+GGG 0.382124 3:-15859291 None:intergenic
GAAGCGTAAAGTTGATTGTT+TGG 0.386718 3:+15857557 MsaT011970.1:CDS
GGGTCGTTTGCGGTGGTCAT+TGG 0.389303 3:-15854218 None:intergenic
GCTAATGTTTCCACATGAAT+AGG 0.389546 3:-15860471 None:intergenic
TTGTAACTCAATAAGATCTT+GGG 0.390486 3:-15859308 None:intergenic
TCAAATCGGTGGATACTAAC+TGG 0.393580 3:+15858449 MsaT011970.1:CDS
AAACAGGAGACTGTATCTTA+TGG 0.393932 3:-15858987 None:intergenic
AATTAAAATTCTATATTCCC+TGG 0.394953 3:+15856706 MsaT011970.1:CDS
AAGAAGGTTGGGATGGTGAA+TGG 0.398183 3:+15854443 MsaT011970.1:CDS
GTTGTAACTCAATAAGATCT+TGG 0.398290 3:-15859309 None:intergenic
TCCACGAAAATCTTTGATAG+TGG 0.398654 3:-15856972 None:intergenic
AAGCTTGTTGATACTTCATA+TGG 0.400306 3:+15857507 MsaT011970.1:CDS
ATTGTCCATAGGTATTATCT+TGG 0.402393 3:-15856642 None:intergenic
GTTGAATGAACAGGATAATT+GGG 0.403727 3:+15859431 MsaT011970.1:intron
GTGAGCCATTAGATTTGGTT+AGG 0.403839 3:+15858102 MsaT011970.1:CDS
CCTGCAAAGCTTTCAACCTC+TGG 0.409697 3:-15859500 None:intergenic
AGAGCTTATGCAGCTGTGAT+TGG 0.410613 3:+15856405 MsaT011970.1:intron
CCTTGTTGCTAGAATGCATT+GGG 0.411990 3:-15859866 None:intergenic
GGTACAGACCACTTTGGGTT+AGG 0.415400 3:-15859286 None:intergenic
CCCCTGTGAGCGGTTCTTCA+AGG 0.417381 3:+15856540 MsaT011970.1:CDS
CCAGCAACAGAACCAGATTT+CGG 0.421076 3:-15854629 None:intergenic
AGATGTGTAAGTTGGCTGTT+AGG 0.423057 3:-15858491 None:intergenic
CTGTTCTTAAAAGACTGTTC+TGG 0.424653 3:-15857714 None:intergenic
CCAGCACGATTCAAATTGTA+TGG 0.427164 3:+15860036 MsaT011970.1:CDS
GGGCCCACTGGACCTCAATA+GGG 0.428655 3:+15856727 MsaT011970.1:CDS
TGTGAATCAGCTTCATGCTT+TGG 0.428794 3:+15854478 MsaT011970.1:CDS
ACAACTGCACTTTGTCCAAC+GGG 0.430181 3:+15857943 MsaT011970.1:CDS
TCATTCCGCATCATCATTCC+AGG 0.431472 3:+15859602 MsaT011970.1:CDS
AGAGAAAACCACAGAGTTTG+TGG 0.431603 3:-15854250 None:intergenic
AAGTAGAGAAAGGTGTATAC+AGG 0.433050 3:+15858015 MsaT011970.1:CDS
TATACATGTCATTGAACAAC+AGG 0.433121 3:+15859695 MsaT011970.1:CDS
CTCTTTCAGAGGAGAAATCA+TGG 0.434595 3:-15857227 None:intergenic
CCACTACTCCAAACCAAAAC+CGG 0.434938 3:-15854596 None:intergenic
AGTATCTTTCTACAGTAGTC+TGG 0.436177 3:-15856455 None:intergenic
ATTTGAATCGTGCTGGAACT+TGG 0.438632 3:-15860029 None:intergenic
CGAAGAAGGTGAAGAAGGTT+GGG 0.440804 3:+15854432 MsaT011970.1:CDS
TATAACCTAACCAAATCTAA+TGG 0.441840 3:-15858107 None:intergenic
CACAAATAGAAAAGCATGTC+AGG 0.443390 3:+15858186 MsaT011970.1:CDS
TCTATATTCCCTGGGCCCAC+TGG 0.443461 3:+15856715 MsaT011970.1:CDS
CAAATTGTTAAATCCTGATC+AGG 0.446046 3:+15857251 MsaT011970.1:CDS
TAGCCGTTTGAGTGCGGAGT+GGG 0.446474 3:+15858301 MsaT011970.1:CDS
TATTTCCAAGATAATACCTA+TGG 0.446650 3:+15856637 MsaT011970.1:CDS
TGTACACTTTGTCTTGTCAA+TGG 0.446929 3:-15859200 None:intergenic
TTTGCCAACTCTTCTTTCGT+TGG 0.447961 3:-15857430 None:intergenic
AGGATAATTGGGATCCTGAT+TGG 0.449928 3:+15859442 MsaT011970.1:intron
ATAATACCTATGGACAATAC+TGG 0.450114 3:+15856647 MsaT011970.1:CDS
TGTTGTGGAGGTTGATTCTA+GGG 0.450644 3:+15854541 MsaT011970.1:CDS
TTTCATTGGTTGCTTCAAAT+CGG 0.451100 3:+15858435 MsaT011970.1:CDS
GCTTGAAATACTTTGTGGTA+TGG 0.451295 3:-15857975 None:intergenic
GATGGATGGGAGTGCTGCAT+TGG 0.453061 3:+15860066 MsaT011970.1:CDS
TGATGTGGCTGCCCTTGGTA+TGG 0.453648 3:+15858073 MsaT011970.1:CDS
ATCTGCGCACTCGCTTGCCT+GGG 0.456519 3:+15858253 MsaT011970.1:CDS
CAGATGGGTCACCAGTAGTC+TGG 0.456981 3:+15857083 MsaT011970.1:CDS
ATAAGATACAGTCTCCTGTT+TGG 0.458193 3:+15858989 MsaT011970.1:CDS
GCAACTTGTTTGTTAAGCTC+AGG 0.460038 3:-15858403 None:intergenic
TTAGATGAAGGGCATACTCT+TGG 0.462268 3:+15858374 MsaT011970.1:CDS
AATTTGCTGGAACAACTACC+AGG 0.463905 3:-15858145 None:intergenic
ATGGATGGGAGTGCTGCATT+GGG 0.465054 3:+15860067 MsaT011970.1:CDS
CATGGGATGCTGGCGTGCTT+AGG 0.467040 3:+15858567 MsaT011970.1:CDS
CAAATGCTTGAAATACTTTG+TGG 0.470118 3:-15857980 None:intergenic
GGTTTAGTGATTTCCTGATC+AGG 0.470225 3:-15857264 None:intergenic
ATTTACCTTCTGTCCCAGAT+TGG 0.471541 3:-15860130 None:intergenic
TGTTCGGCGTAATATATTGA+TGG 0.471628 3:+15858763 MsaT011970.1:CDS
ATTAAAATTCTATATTCCCT+GGG 0.473601 3:+15856707 MsaT011970.1:CDS
GATTGTTGATGTTTATCTTC+CGG 0.475214 3:+15854577 MsaT011970.1:CDS
AGAAGGTTGGGATGGTGAAT+GGG 0.475682 3:+15854444 MsaT011970.1:CDS
CACCAGTTACAGTATTTACA+TGG 0.476766 3:-15856928 None:intergenic
CGAGAGTTCGAGTGCGAAGA+AGG 0.476982 3:+15854418 MsaT011970.1:CDS
ATCAGCAGGCAGCAGTTGAA+TGG 0.479240 3:+15856825 MsaT011970.1:CDS
CTTGCAGAAATGGAAACAAA+TGG 0.479957 3:+15857900 MsaT011970.1:CDS
ATGTCGACATGGCGTAATGA+AGG 0.480995 3:-15859155 None:intergenic
TCCCTTGAAGAACCGCTCAC+AGG 0.482011 3:-15856542 None:intergenic
TAACTGGTGATATAGTCATC+GGG 0.482511 3:+15856942 MsaT011970.1:CDS
TATTCCTCAGAGCAAGCATC+AGG 0.483783 3:-15857324 None:intergenic
TGACACAGTGGCCACCAAAC+AGG 0.484233 3:-15859003 None:intergenic
GTCCATAAGCTTCTTCATGA+AGG 0.485696 3:-15858532 None:intergenic
TATTGAGGTCCAGTGGGCCC+AGG 0.485759 3:-15856724 None:intergenic
ATGCATGATTAGTGCTAGAA+AGG 0.486577 3:+15858646 MsaT011970.1:CDS
ATGGTCTAGATTCTACTTCA+GGG 0.489153 3:+15858954 MsaT011970.1:CDS
ACACGCTGGAGAAGATATTC+AGG 0.491050 3:+15858907 MsaT011970.1:CDS
AATTAGTCGTGCCCATCGTA+TGG 0.491970 3:+15860437 MsaT011970.1:CDS
TGGCGTGCTTAGGCCATTCG+AGG 0.492502 3:+15858577 MsaT011970.1:CDS
AAATTGTATGGCACTTCTGA+TGG 0.494975 3:+15860048 MsaT011970.1:CDS
CCCTTGAAGAACCGCTCACA+GGG 0.495081 3:-15856541 None:intergenic
CAGGCAAGCGAGTGCGCAGA+TGG 0.495564 3:-15858251 None:intergenic
CAGACTACTGGTGACCCATC+TGG 0.497077 3:-15857082 None:intergenic
TTACCAGGATTTCACTTGAA+AGG 0.498175 3:+15857753 MsaT011970.1:CDS
GATGCAAAGTCCTGATTCAA+TGG 0.499559 3:+15859249 MsaT011970.1:CDS
TTTATGTTTGGAACGATCAT+CGG 0.500097 3:+15858225 MsaT011970.1:CDS
CTGTTAGGAGTGTTCGATGT+TGG 0.500631 3:-15858476 None:intergenic
AAATCTGTTTCCTCATCAGC+AGG 0.501336 3:+15856811 MsaT011970.1:CDS
TCAACTGCTGCCTGCTGATG+AGG 0.502624 3:-15856821 None:intergenic
ACTCTACAACTCTGATACAA+AGG 0.503506 3:-15857690 None:intergenic
ACCTTGTTGCTAGAATGCAT+TGG 0.503718 3:-15859867 None:intergenic
AACAGATTTAACTTTGTGCA+AGG 0.507122 3:-15856797 None:intergenic
CTTCTAGTGCACCGACTCGC+AGG 0.507703 3:-15857351 None:intergenic
CCATACAATTTGAATCGTGC+TGG 0.508271 3:-15860036 None:intergenic
ACTACTCATTGGTAAATTCT+CGG 0.510262 3:+15857832 MsaT011970.1:CDS
GTAATATATTGATGGCTGAT+TGG 0.511643 3:+15858771 MsaT011970.1:CDS
GGCCCCACTCCGCACTCAAA+CGG 0.512630 3:-15858304 None:intergenic
GTTCCTTTCAAGTGAAATCC+TGG 0.515719 3:-15857756 None:intergenic
GCTTAAGGAGCACTACTCAT+TGG 0.516177 3:+15857821 MsaT011970.1:CDS
ATAGAAAAGCATGTCAGGCC+TGG 0.517076 3:+15858191 MsaT011970.1:CDS
TCTTCCATCTCTGCCTCGAA+TGG 0.518095 3:-15858590 None:intergenic
CCCTGTGAGCGGTTCTTCAA+GGG 0.522105 3:+15856541 MsaT011970.1:CDS
AAGAACCGCTCACAGGGGAA+TGG 0.522294 3:-15856535 None:intergenic
AACAACTGCACTTTGTCCAA+CGG 0.522924 3:+15857942 MsaT011970.1:CDS
GACTACTGTAGAAAGATACT+TGG 0.523086 3:+15856458 MsaT011970.1:CDS
AGCCATGCCTGCGCATAAGA+AGG 0.527424 3:-15859331 None:intergenic
TCTTGGGGTACAGACCACTT+TGG 0.527858 3:-15859292 None:intergenic
TGGACAATACTGGCGGATCT+GGG 0.528838 3:+15856657 MsaT011970.1:CDS
AATGTGATGCTTGTCACAAA+TGG 0.532521 3:+15857601 MsaT011970.1:CDS
CTCTGCTGATTTCCTACACA+AGG 0.532791 3:-15856608 None:intergenic
AAAACAACTTTCTTGATGCA+TGG 0.533458 3:-15858689 None:intergenic
AAGACAAAGTGTACATATCC+TGG 0.533923 3:+15859208 MsaT011970.1:CDS
CACAAAGACGCAAGGTACAT+TGG 0.534529 3:+15857051 MsaT011970.1:CDS
CACATCTACAACCATTGCTA+AGG 0.537808 3:+15858507 MsaT011970.1:CDS
ATTTCCAACGAAAGAAGAGT+TGG 0.540007 3:+15857426 MsaT011970.1:CDS
ATGAGTTGGTACTCACTGTT+CGG 0.540678 3:+15858747 MsaT011970.1:CDS
AGGAATTACTGAAGCAGCCA+AGG 0.540726 3:-15856777 None:intergenic
CCTCATGATTCATGTTCTGT+AGG 0.541036 3:+15857285 MsaT011970.1:CDS
CATTGGTTGCTTCAAATCGG+TGG 0.542676 3:+15858438 MsaT011970.1:CDS
AGGAATATTCGCCTGCGAGT+CGG 0.543870 3:+15857340 MsaT011970.1:CDS
GTTACAACCTTCTTATGCGC+AGG 0.544047 3:+15859324 MsaT011970.1:CDS
ATCAGTGGTAATAACACGGC+TGG 0.544193 3:+15857145 MsaT011970.1:CDS
GTTACAGTCTTACCCAAGCC+AGG 0.544684 3:-15857013 None:intergenic
ATCGCGTTTCGTACTCAAAA+CGG 0.544800 3:+15854356 MsaT011970.1:CDS
GGTTGAGAGAGGGTCGTTTG+CGG 0.545076 3:-15854228 None:intergenic
GAGAAAACCACAGAGTTTGT+GGG 0.545584 3:-15854249 None:intergenic
CTCTACAACTCTGATACAAA+GGG 0.545584 3:-15857689 None:intergenic
GCAGCTCTATCAACTGATGA+TGG 0.546385 3:+15856896 MsaT011970.1:CDS
ATTGGTAAATTCTCGGACAA+AGG 0.547067 3:+15857839 MsaT011970.1:CDS
ATTTCACTTGAAAGGAACTC+CGG 0.547183 3:+15857761 MsaT011970.1:CDS
GACAGAGTTACAAGTGGCAT+GGG 0.550843 3:+15857535 MsaT011970.1:CDS
CAGAGTTTGTGGGTTGAGAG+AGG 0.551453 3:-15854239 None:intergenic
TAGGCCATTCGAGGCAGAGA+TGG 0.555199 3:+15858586 MsaT011970.1:CDS
CCCAATGCATTCTAGCAACA+AGG 0.557176 3:+15859866 MsaT011970.1:CDS
TGTATGGCACTTCTGATGGA+TGG 0.557768 3:+15860052 MsaT011970.1:CDS
ATTGAGGTCCAGTGGGCCCA+GGG 0.559176 3:-15856723 None:intergenic
TCCTACACAAGGGGAAGGTA+CGG 0.560133 3:-15856597 None:intergenic
GGACAAAGGAGGCTCTGACT+TGG 0.560926 3:+15857853 MsaT011970.1:CDS
GAGTGCGAAGAAGGTGAAGA+AGG 0.561114 3:+15854427 MsaT011970.1:CDS
TCTAAGATAACTTACTTACC+AGG 0.562932 3:+15857738 MsaT011970.1:CDS
TACATTTAGCCGTTTGAGTG+CGG 0.563559 3:+15858295 MsaT011970.1:CDS
CCACGAAAATCTTTGATAGT+GGG 0.564131 3:-15856971 None:intergenic
GGACAGAGTTACAAGTGGCA+TGG 0.565878 3:+15857534 MsaT011970.1:CDS
GAAGGTGAAGAAGGTTGGGA+TGG 0.566498 3:+15854436 MsaT011970.1:CDS
GTGCTCTAATGCAAGTGCAC+TGG 0.567512 3:+15858339 MsaT011970.1:CDS
CGGAGGGGTTAGTGTGAGAA+CGG 0.567994 3:-15854276 None:intergenic
ATTAGAGCACTCTTCTTACG+TGG 0.568097 3:-15858326 None:intergenic
GTAACTGGTGATATAGTCAT+CGG 0.568117 3:+15856941 MsaT011970.1:CDS
AGATACAGTCTCCTGTTTGG+TGG 0.570067 3:+15858992 MsaT011970.1:CDS
AGAGCTGCATATAAAGGATG+TGG 0.570630 3:-15856881 None:intergenic
AGAGTTTGTGGGTTGAGAGA+GGG 0.570895 3:-15854238 None:intergenic
TGGAGCCAATCTGGGACAGA+AGG 0.571632 3:+15860125 MsaT011970.1:CDS
GATGACAACATCATAATCCC+AGG 0.571751 3:-15858270 None:intergenic
TTATGAATTTCGTAGGTGCA+AGG 0.572108 3:+15859118 MsaT011970.1:intron
TGAGAGAGGGTCGTTTGCGG+TGG 0.573498 3:-15854225 None:intergenic
ATGTCATCATCTAAGAGCCT+TGG 0.574388 3:+15856760 MsaT011970.1:CDS
TGCGTCCCGTCCTATTCATG+TGG 0.574636 3:+15860461 MsaT011970.1:CDS
TAACAATTTGGCTCTTTCAG+AGG 0.574738 3:-15857238 None:intergenic
TTCGAGGCAGAGATGGAAGA+AGG 0.574892 3:+15858593 MsaT011970.1:CDS
TCACAAATGGCGAAAGCTTG+CGG 0.575828 3:+15857614 MsaT011970.1:CDS
CAGAAAATCCTAACCCAAAG+TGG 0.576600 3:+15859278 MsaT011970.1:CDS
AAAGGAGGCTCTGACTTGGT+TGG 0.577350 3:+15857857 MsaT011970.1:CDS
TTTCACTTGAAAGGAACTCC+GGG 0.578633 3:+15857762 MsaT011970.1:CDS
TCATACAAGTTATCACAGCC+AGG 0.581057 3:-15859226 None:intergenic
TTATGTTGACACCAGACTAC+TGG 0.582238 3:-15857094 None:intergenic
ATTAGTCGTGCCCATCGTAT+GGG 0.583520 3:+15860438 MsaT011970.1:CDS
AGAAGATATTCAGGAAACAA+TGG 0.583877 3:+15858916 MsaT011970.1:CDS
TGAGCGGTTCTTCAAGGGAA+AGG 0.588938 3:+15856546 MsaT011970.1:CDS
TGTCAATGGAAACACAATCA+AGG 0.590521 3:-15859186 None:intergenic
ACATTGGCGGATCCACCAGA+TGG 0.590534 3:+15857067 MsaT011970.1:CDS
GATGTTGTCAAATCTGAAGA+TGG 0.590681 3:+15860752 MsaT011970.1:CDS
ATACTTGTCAAGAGCAACCC+TGG 0.590847 3:+15858127 MsaT011970.1:CDS
GACGGGACGCACCCATACGA+TGG 0.591504 3:-15860449 None:intergenic
TGTTTCCACATGAATAGGAC+GGG 0.593054 3:-15860466 None:intergenic
TCATGAAGGAACCTTAGCAA+TGG 0.594391 3:-15858518 None:intergenic
TTGTCAAATCTGAAGATGGT+GGG 0.595354 3:+15860756 MsaT011970.1:CDS
TGCTAGAAGTTACAATGAGT+TGG 0.601484 3:+15858733 MsaT011970.1:CDS
CAGAGGTTGAAAGCTTTGCA+GGG 0.604660 3:+15859501 MsaT011970.1:CDS
AGCCGTTTGAGTGCGGAGTG+GGG 0.605704 3:+15858302 MsaT011970.1:CDS
TGTCGACATGGCGTAATGAA+GGG 0.607588 3:-15859154 None:intergenic
AAGCTTGCGGATAACAGTAT+GGG 0.608301 3:+15857627 MsaT011970.1:CDS
GATCCGCCAGTATTGTCCAT+AGG 0.610382 3:-15856653 None:intergenic
TCTCTTATCACAAAGACGCA+AGG 0.612018 3:+15857043 MsaT011970.1:CDS
CATTGGCGGATCCACCAGAT+GGG 0.612044 3:+15857068 MsaT011970.1:CDS
ACAACTTTCTTGATGCATGG+TGG 0.612301 3:-15858686 None:intergenic
ACGGGACGCACCCATACGAT+GGG 0.612774 3:-15860448 None:intergenic
GGTAAATTCTCGGACAAAGG+AGG 0.613744 3:+15857842 MsaT011970.1:CDS
GAAACCCTATTGAGGTCCAG+TGG 0.614244 3:-15856731 None:intergenic
GTATGGATTCACAGTCCCGT+TGG 0.614716 3:-15857958 None:intergenic
AAAGACGCAAGGTACATTGG+CGG 0.619575 3:+15857054 MsaT011970.1:CDS
GTATGGCACTTCTGATGGAT+GGG 0.620158 3:+15860053 MsaT011970.1:CDS
AAACCCTATTGAGGTCCAGT+GGG 0.622694 3:-15856730 None:intergenic
ATGTTGCAGAAACCCTATTG+AGG 0.623777 3:-15856739 None:intergenic
CATATTAAAGTTACACACGC+TGG 0.624968 3:+15858893 MsaT011970.1:CDS
ATGTTTCCACATGAATAGGA+CGG 0.627738 3:-15860467 None:intergenic
ATGGTTGTAGATGTGTAAGT+TGG 0.627824 3:-15858499 None:intergenic
AAGCTCCATTCCCCTGTGAG+CGG 0.628644 3:+15856530 MsaT011970.1:CDS
AGTAGACTTTCCACATGCGA+AGG 0.629550 3:-15858800 None:intergenic
GTCTACTGAATCCAAAACAG+TGG 0.630419 3:+15858816 MsaT011970.1:CDS
AGTGGTAATAACACGGCTGG+TGG 0.634167 3:+15857148 MsaT011970.1:CDS
CAGGAGACTGTATCTTATGG+AGG 0.634908 3:-15858984 None:intergenic
AAAGTCCTGATTCAATGGCA+CGG 0.635118 3:+15859254 MsaT011970.1:CDS
TTAGAGCACTCTTCTTACGT+GGG 0.635425 3:-15858325 None:intergenic
CGAGATCAGTGGTAATAACA+CGG 0.636732 3:+15857141 MsaT011970.1:CDS
ACCGTACCTTCCCCTTGTGT+AGG 0.636865 3:+15856596 MsaT011970.1:CDS
ACCTCCTGATGCTTGCTCTG+AGG 0.637926 3:+15857320 MsaT011970.1:CDS
CTCTCAACCCACAAACTCTG+TGG 0.640032 3:+15854242 MsaT011970.1:CDS
ACATAAACATGCAATTGACC+AGG 0.640310 3:-15858209 None:intergenic
AGGCAGAGATGGAAGAAGGT+CGG 0.641182 3:+15858597 MsaT011970.1:CDS
TGGTCTAGATTCTACTTCAG+GGG 0.641369 3:+15858955 MsaT011970.1:CDS
GCTCACAGAGAGCCATACCA+AGG 0.642203 3:-15858085 None:intergenic
TGTCAAATCTGAAGATGGTG+GGG 0.643211 3:+15860757 MsaT011970.1:CDS
AATTTCGTAGGTGCAAGGAA+TGG 0.643325 3:+15859123 MsaT011970.1:intron
TAGAGAAAGGTGTATACAGG+TGG 0.643392 3:+15858018 MsaT011970.1:CDS
TCCTCAGAGCAAGCATCAGG+AGG 0.643607 3:-15857321 None:intergenic
TGGGTAATTCTAATGCAGCA+TGG 0.644906 3:+15857646 MsaT011970.1:CDS
TGTAACTCAATAAGATCTTG+GGG 0.649776 3:-15859307 None:intergenic
CTGCTGATTTCCTACACAAG+GGG 0.650157 3:-15856606 None:intergenic
TTGGAATGATCCTTCGCATG+TGG 0.651252 3:+15858790 MsaT011970.1:CDS
CACTTGAAAGGAACTCCGGG+TGG 0.654146 3:+15857765 MsaT011970.1:CDS
CCTACAGAACATGAATCATG+AGG 0.654419 3:-15857285 None:intergenic
CATGGCGTAATGAAGGGAGT+CGG 0.663299 3:-15859148 None:intergenic
GTGGAAACATTAGCTATGCG+TGG 0.666167 3:+15860480 MsaT011970.1:CDS
GTTTGGAGTACAGTGATACG+TGG 0.666295 3:+15857574 MsaT011970.1:CDS
TGTGGCTATTACGAGATCAG+TGG 0.666939 3:+15857130 MsaT011970.1:CDS
ACTACTGGTGACCCATCTGG+TGG 0.667483 3:-15857079 None:intergenic
GCGGAATGACTTGTGTGCAA+AGG 0.667601 3:-15859588 None:intergenic
GTCAAATCTGAAGATGGTGG+GGG 0.669627 3:+15860758 MsaT011970.1:CDS
TCTATATTTCACAGATGACG+AGG 0.671046 3:+15857395 MsaT011970.1:CDS
TTAGCCGTTTGAGTGCGGAG+TGG 0.671278 3:+15858300 MsaT011970.1:CDS
ATACCTATGGACAATACTGG+CGG 0.671595 3:+15856650 MsaT011970.1:CDS
TCTGCTGATTTCCTACACAA+GGG 0.673987 3:-15856607 None:intergenic
GTTGTCAAATCTGAAGATGG+TGG 0.674583 3:+15860755 MsaT011970.1:CDS
CTCACAGAGAGCCATACCAA+GGG 0.681432 3:-15858084 None:intergenic
TGATTTCCTACACAAGGGGA+AGG 0.681549 3:-15856602 None:intergenic
AGGCACAACATATGTCGACA+TGG 0.683756 3:-15859166 None:intergenic
ATTATCGATTCAACGCAATG+CGG 0.695186 3:+15854395 MsaT011970.1:CDS
AAGTTGCATATCTCGTCCAG+AGG 0.698811 3:+15859484 MsaT011970.1:CDS
ATTTGCTGGAACAACTACCA+GGG 0.699718 3:-15858144 None:intergenic
AACTGGTGATATAGTCATCG+GGG 0.706873 3:+15856943 MsaT011970.1:CDS
ACATTCCTGGAATGATGATG+CGG 0.709579 3:-15859607 None:intergenic
GTTCTGGACAGAGTTACAAG+TGG 0.713387 3:+15857529 MsaT011970.1:CDS
AAACAGTGGAAATTTCGGAG+TGG 0.715062 3:+15858830 MsaT011970.1:CDS
AAGTAGCATTAGTACCAATG+TGG 0.718006 3:+15859632 MsaT011970.1:CDS
CCTTGAAGAACCGCTCACAG+GGG 0.734849 3:-15856540 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 15854221 15860847 15854221 ID=MsaG011970
Chr3 mRNA 15854221 15860847 15854221 ID=MsaT011970.1;Parent=MsaG011970
Chr3 exon 15854221 15854672 15854221 ID=MsaT011970.1.exon1;Parent=MsaT011970.1
Chr3 CDS 15854221 15854672 15854221 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15856418 15857169 15856418 ID=MsaT011970.1.exon2;Parent=MsaT011970.1
Chr3 CDS 15856418 15857169 15856418 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15857205 15859021 15857205 ID=MsaT011970.1.exon3;Parent=MsaT011970.1
Chr3 CDS 15857205 15859021 15857205 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15859133 15859345 15859133 ID=MsaT011970.1.exon4;Parent=MsaT011970.1
Chr3 CDS 15859133 15859345 15859133 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15859444 15859716 15859444 ID=MsaT011970.1.exon5;Parent=MsaT011970.1
Chr3 CDS 15859444 15859716 15859444 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15859828 15859887 15859828 ID=MsaT011970.1.exon6;Parent=MsaT011970.1
Chr3 CDS 15859828 15859887 15859828 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15860016 15860146 15860016 ID=MsaT011970.1.exon7;Parent=MsaT011970.1
Chr3 CDS 15860016 15860146 15860016 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15860422 15860536 15860422 ID=MsaT011970.1.exon8;Parent=MsaT011970.1
Chr3 CDS 15860422 15860536 15860422 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Chr3 exon 15860719 15860847 15860719 ID=MsaT011970.1.exon9;Parent=MsaT011970.1
Chr3 CDS 15860719 15860847 15860719 ID=cds.MsaT011970.1;Parent=MsaT011970.1
Gene Sequence

>MsaT011970.1

ATGACCACCGCAAACGACCCTCTCTCAACCCACAAACTCTGTGGTTTTCTCTCAACCGTTCTCACACTAACCCCTCCGAATCAAAACGACACGCGTTTTAACGATCGATGCGAATTCTTCAACGATAACAATACAATCGCGTTTCGTACTCAAAACGGTGTCGTTTTGAATCCAATTATCGATTCAACGCAATGCGGCGAGAGTTCGAGTGCGAAGAAGGTGAAGAAGGTTGGGATGGTGAATGGGAGTTTCAGTGTTGTGAATCAGCTTCATGCTTTGGTTTTGAGGAAATGTTTGGATATTGATGCACGTGTTTTGTGTGTTGTGGAGGTTGATTCTAGGGTTAGGGTTGTTGTGATTGTTGATGTTTATCTTCCGGTTTTGGTTTGGAGTAGTGGGTGGCAGTTTCCGAAATCTGGTTCTGTTGCTGGTGCTGTTTTTCGACATTTGAGCTGTGATTGGAATGAGAGAAGTTCAATGCTTTCTGATCCAGACTACTGTAGAAAGATACTTGGAGAAAATGAGAGCATTTGGAATCTTTCTGATTGTCATGTACTTGGTTGCAAGCTCCATTCCCCTGTGAGCGGTTCTTCAAGGGAAAGGCTATTTGAACTTCATGAAATTTTTAAGACCGTACCTTCCCCTTGTGTAGGAAATCAGCAGAGCTTTCTTATTTCCAAGATAATACCTATGGACAATACTGGCGGATCTGGGATTTGGGATATCTCTGATGATATTTTAATTAAAATTCTATATTCCCTGGGCCCACTGGACCTCAATAGGGTTTCTGCAACATGTCATCATCTAAGAGCCTTGGCTGCTTCAGTAATTCCTTGCACAAAGTTAAATCTGTTTCCTCATCAGCAGGCAGCAGTTGAATGGATGTTGCAACGTGAACGAAATGCTGAACTTATGCCACATCCTTTATATGCAGCTCTATCAACTGATGATGGCTTTAGTTTCCATGTAAATACTGTAACTGGTGATATAGTCATCGGGGAAACTCCCACTATCAAAGATTTTCGTGGAGGAATGTTTTGTGATGAGCCTGGCTTGGGTAAGACTGTAACAGCTCTTTCTCTTATCACAAAGACGCAAGGTACATTGGCGGATCCACCAGATGGGTCACCAGTAGTCTGGTGTCAACATAACACTAACAAAAAATGTGGCTATTACGAGATCAGTGGTAATAACACGGCTGGTGATTCAGGTAGAAGTAATGAGAACCATGATTTCTCCTCTGAAAGAGCCAAATTGTTAAATCCTGATCAGGAAATCACTAAACCTCATGATTCATGTTCTGTAGGAGAAGACAAATCACCTCCTGATGCTTGCTCTGAGGAATATTCGCCTGCGAGTCGGTGCACTAGAAGCTTGAGTCGCGTAAAGAAAAATCTATATTTCACAGATGACGAGGAAGCTATGATTTCCAACGAAAGAAGAGTTGGCAAAAGACCAATTAAAACAAAACATGCATCAGATGTTGCATCCCAGATGTCTCAAAACAAGCTTGTTGATACTTCATATGGTTCTGGACAGAGTTACAAGTGGCATGGGAAGCGTAAAGTTGATTGTTTGGAGTACAGTGATACGTGGATTCAATGTGATGCTTGTCACAAATGGCGAAAGCTTGCGGATAACAGTATGGGTAATTCTAATGCAGCATGGTTTTGTAGTATGAACACTGACCCTTTGTATCAGAGTTGTAGAGTTCCAGAACAGTCTTTTAAGAACAGCTCTAAGATAACTTACTTACCAGGATTTCACTTGAAAGGAACTCCGGGTGGTGAGAAACAAAACGTTTCATTTTTCACTAGTGTGCTTAAGGAGCACTACTCATTGGTAAATTCTCGGACAAAGGAGGCTCTGACTTGGTTGGCCAAAATTTCTATTGACAAGCTTGCAGAAATGGAAACAAATGGAATGCGAAGTCCTATTTTAAACAACTGCACTTTGTCCAACGGGACTGTGAATCCATACCACAAAGTATTTCAAGCATTTGGCCTAAAAAAGAAAGTAGAGAAAGGTGTATACAGGTGGTTCTACCCAAAAAATCTTAAAAATTTGACTTTTGATGTGGCTGCCCTTGGTATGGCTCTCTGTGAGCCATTAGATTTGGTTAGGTTATACTTGTCAAGAGCAACCCTGGTAGTTGTTCCAGCAAATTTGGTTGATCATTGGAAAGCACAAATAGAAAAGCATGTCAGGCCTGGTCAATTGCATGTTTATGTTTGGAACGATCATCGGAAACCATCTGCGCACTCGCTTGCCTGGGATTATGATGTTGTCATCACTACATTTAGCCGTTTGAGTGCGGAGTGGGGCCCACGTAAGAAGAGTGCTCTAATGCAAGTGCACTGGTTTAGGATAATTTTAGATGAAGGGCATACTCTTGGCTCAAGCCTGAGCTTAACAAACAAGTTGCAAATGACTATTTCATTGGTTGCTTCAAATCGGTGGATACTAACTGGAACTCCAACATCGAACACTCCTAACAGCCAACTTACACATCTACAACCATTGCTAAGGTTCCTTCATGAAGAAGCTTATGGACTGAATCAAAAATCATGGGATGCTGGCGTGCTTAGGCCATTCGAGGCAGAGATGGAAGAAGGTCGGTCTCGTCTGTTGCATTTACTTCAGAAATGCATGATTAGTGCTAGAAAGGCAGATTTGCAAAGTATTCCACCATGCATCAAGAAAGTTGTTTTACTAGATTTTAATGAGGAACATGCTAGAAGTTACAATGAGTTGGTACTCACTGTTCGGCGTAATATATTGATGGCTGATTGGAATGATCCTTCGCATGTGGAAAGTCTACTGAATCCAAAACAGTGGAAATTTCGGAGTGGAGCTATAAAAAATGTAAGGCTTTCATGCTGTGTTGCTGGACATATTAAAGTTACACACGCTGGAGAAGATATTCAGGAAACAATGGATATGTTAGTGAAAAATGGTCTAGATTCTACTTCAGGGGAGTATACCTCCATAAGATACAGTCTCCTGTTTGGTGGCCACTGTGTGCAAGGAATGGTGCCGACTCCCTTCATTACGCCATGTCGACATATGTTGTGCCTTGATTGTGTTTCCATTGACAAGACAAAGTGTACATATCCTGGCTGTGATAACTTGTATGAGATGCAAAGTCCTGATTCAATGGCACGGCCAGAAAATCCTAACCCAAAGTGGTCTGTACCCCAAGATCTTATTGAGTTACAACCTTCTTATGCGCAGGATAATTGGGATCCTGATTGGCAATCAACATCTAGTAGTAAAGTTGCATATCTCGTCCAGAGGTTGAAAGCTTTGCAGGGAATCAATGAAGAGATGAGTTCATATACTGACAATAGCAATGCGATGTATATTGAAAATAGTTTTCCTTTGCACACAAGTCATTCCGCATCATCATTCCAGGAATGTTCAAGTAGCATTAGTACCAATGTGGTCCCTGAAAAAGTTTTGATATTTTCTCAGTTTCTTGAGCATATACATGTCATTGAACAACAGTTGGCTGTTAATGGTATCAAATATACTGGAATGTATAACCCAATGCATTCTAGCAACAAGAAGAAGTCATTAGCCAAGTTCCAGCACGATTCAAATTGTATGGCACTTCTGATGGATGGGAGTGCTGCATTGGGTCTTGACTTAAGTTTTGTTACACATGTATTTTTAATGGAGCCAATCTGGGACAGAAGTATGGAGGAGCAAGTAATTAGTCGTGCCCATCGTATGGGTGCGTCCCGTCCTATTCATGTGGAAACATTAGCTATGCGTGGTACAATTGAAGAGCAAATGCTAGAATTTTTACAGGATGCTGACAAACGTAGAAGATTTCATAATAAAGATGTTGTCAAATCTGAAGATGGTGGGGGACGAGGGTGTCGATCAGTGCATGATTTTGCTGAAAGCAATTATCTACTGGAACTTAAATTTGTATAA
Protein sequence

>MsaT011970.1

MTTANDPLSTHKLCGFLSTVLTLTPPNQNDTRFNDRCEFFNDNNTIAFRTQNGVVLNPIIDSTQCGESSSAKKVKKVGMVNGSFSVVNQLHALVLRKCLDIDARVLCVVEVDSRVRVVVIVDVYLPVLVWSSGWQFPKSGSVAGAVFRHLSCDWNERSSMLSDPDYCRKILGENESIWNLSDCHVLGCKLHSPVSGSSRERLFELHEIFKTVPSPCVGNQQSFLISKIIPMDNTGGSGIWDISDDILIKILYSLGPLDLNRVSATCHHLRALAASVIPCTKLNLFPHQQAAVEWMLQRERNAELMPHPLYAALSTDDGFSFHVNTVTGDIVIGETPTIKDFRGGMFCDEPGLGKTVTALSLITKTQGTLADPPDGSPVVWCQHNTNKKCGYYEISGNNTAGDSGRSNENHDFSSERAKLLNPDQEITKPHDSCSVGEDKSPPDACSEEYSPASRCTRSLSRVKKNLYFTDDEEAMISNERRVGKRPIKTKHASDVASQMSQNKLVDTSYGSGQSYKWHGKRKVDCLEYSDTWIQCDACHKWRKLADNSMGNSNAAWFCSMNTDPLYQSCRVPEQSFKNSSKITYLPGFHLKGTPGGEKQNVSFFTSVLKEHYSLVNSRTKEALTWLAKISIDKLAEMETNGMRSPILNNCTLSNGTVNPYHKVFQAFGLKKKVEKGVYRWFYPKNLKNLTFDVAALGMALCEPLDLVRLYLSRATLVVVPANLVDHWKAQIEKHVRPGQLHVYVWNDHRKPSAHSLAWDYDVVITTFSRLSAEWGPRKKSALMQVHWFRIILDEGHTLGSSLSLTNKLQMTISLVASNRWILTGTPTSNTPNSQLTHLQPLLRFLHEEAYGLNQKSWDAGVLRPFEAEMEEGRSRLLHLLQKCMISARKADLQSIPPCIKKVVLLDFNEEHARSYNELVLTVRRNILMADWNDPSHVESLLNPKQWKFRSGAIKNVRLSCCVAGHIKVTHAGEDIQETMDMLVKNGLDSTSGEYTSIRYSLLFGGHCVQGMVPTPFITPCRHMLCLDCVSIDKTKCTYPGCDNLYEMQSPDSMARPENPNPKWSVPQDLIELQPSYAQDNWDPDWQSTSSSKVAYLVQRLKALQGINEEMSSYTDNSNAMYIENSFPLHTSHSASSFQECSSSISTNVVPEKVLIFSQFLEHIHVIEQQLAVNGIKYTGMYNPMHSSNKKKSLAKFQHDSNCMALLMDGSAALGLDLSFVTHVFLMEPIWDRSMEEQVISRAHRMGASRPIHVETLAMRGTIEEQMLEFLQDADKRRRFHNKDVVKSEDGGGRGCRSVHDFAESNYLLELKFV*