AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG011971


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011971 MtrunA17_Chr3g0083491 78.926 503 39 6 1 445 728 1221 0.0 756
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG011971 AT3G54460.2 56.275 510 143 10 1 445 455 949 1.34e-175 520
MsaG011971 AT3G54460.1 56.275 510 143 10 1 445 752 1246 7.15e-173 521

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGAATGAACAGGATAAT+TGG 0.223532 3:+15916647 MsaT011971.1:intron
CTACGAAATTTCCATTGTTT+TGG 0.236191 3:-15916044 None:intergenic
CTTGGGGTACAAACCACTTT+AGG 0.270323 3:-15916508 None:intergenic
CCAGAGGTTGAAAGCTTTGC+AGG 0.299028 3:+15916717 MsaT011971.1:CDS
TTCCTTCATGAAGAAGCTTA+TGG 0.308034 3:+15915747 MsaT011971.1:CDS
TCTGGCCGTGCCATTGAATC+AGG 0.311287 3:-15916476 None:intergenic
CAGCAAATTTGGTCGATCAT+TGG 0.322167 3:+15915374 MsaT011971.1:CDS
AGGCTTTCATGCTGTGTTGC+TGG 0.327727 3:+15916086 MsaT011971.1:CDS
CTAGATGTTGATTGCCAATC+AGG 0.333695 3:-15916673 None:intergenic
GGTAGTTGTTCCAGCAAATT+TGG 0.341778 3:+15915363 MsaT011971.1:CDS
CATCTGCGTACTCGCTTGCC+TGG 0.344647 3:+15915467 MsaT011971.1:CDS
CAATGATCGACCAAATTTGC+TGG 0.346850 3:-15915373 None:intergenic
TGTCAGGCCTGCTCAATTGC+AGG 0.356817 3:+15915417 MsaT011971.1:CDS
GTTAATGGTATCAAATATAC+TGG 0.364522 3:+15917050 MsaT011971.1:CDS
TAATGCTACTTGAACATTCC+TGG 0.371689 3:-15916837 None:intergenic
TTCGAGGCAGAGATGGAAAA+AGG 0.379826 3:+15915810 MsaT011971.1:CDS
GTTGCAAATGACTATTTCAT+TGG 0.380919 3:+15915636 MsaT011971.1:intron
ATATGAACTCATCTCTTCAT+TGG 0.383511 3:-15916743 None:intergenic
TTGTAACTCAATAAGATCTT+GGG 0.390486 3:-15916525 None:intergenic
TCAAATCGGTGGATACTAAC+TGG 0.393580 3:+15915664 MsaT011971.1:CDS
GTTGTAACTCAATAAGATCT+TGG 0.398290 3:-15916526 None:intergenic
GTTAGGAGTGTTTGATGTGT+TGG 0.401806 3:-15915691 None:intergenic
GTTGAATGAACAGGATAATT+GGG 0.403727 3:+15916648 MsaT011971.1:intron
CCTGCAAAGCTTTCAACCTC+TGG 0.408088 3:-15916717 None:intergenic
CCTTGTTGCTAGAATGCATT+GGG 0.411990 3:-15917082 None:intergenic
AGATGTGTAAGTTGGCTGTT+AGG 0.423057 3:-15915708 None:intergenic
TACAAATAGAAAAGCATGTC+AGG 0.427551 3:+15915401 MsaT011971.1:CDS
TCATTCCGCATCATCATTCC+AGG 0.431472 3:+15916819 MsaT011971.1:CDS
ACTTGAATCGTGCTGGAACT+TGG 0.432061 3:-15917244 None:intergenic
TATACATGTCATTGAACAAC+AGG 0.433121 3:+15916912 MsaT011971.1:CDS
TAGCTGTTTGAGTGCGGAGT+GGG 0.435475 3:+15915516 MsaT011971.1:CDS
GGTACAAACCACTTTAGGTT+AGG 0.437675 3:-15916503 None:intergenic
AGGATAATTGGGATCCTGAT+TGG 0.449928 3:+15916659 MsaT011971.1:intron
TTTCATTGGTTGCTTCAAAT+CGG 0.451100 3:+15915650 MsaT011971.1:CDS
AATTTGCTGGAACAACTACC+AGG 0.463905 3:-15915360 None:intergenic
CATGGGATGCTGGCGTGCTT+AGG 0.467040 3:+15915784 MsaT011971.1:CDS
TGTACACTTGGTCTTGTCAA+TGG 0.468799 3:-15916417 None:intergenic
TGTTCGGCGTAATATATTGA+TGG 0.471628 3:+15915980 MsaT011971.1:CDS
TTTATGTTTGTAACGATCAT+CGG 0.474244 3:+15915440 MsaT011971.1:CDS
GTCCATAAGCTTCTTCATGA+AGG 0.485696 3:-15915749 None:intergenic
ATGCATGATTAGTGCTAGAA+AGG 0.486577 3:+15915863 MsaT011971.1:CDS
ACACGCTGGAGAAGATATTC+AGG 0.491050 3:+15916124 MsaT011971.1:CDS
CAGAAAATCCTAACCTAAAG+TGG 0.491218 3:+15916495 MsaT011971.1:CDS
TGGCGTGCTTAGGCCATTCG+AGG 0.492502 3:+15915794 MsaT011971.1:CDS
ACCTTGTTGCTAGAATGCAT+TGG 0.503718 3:-15917083 None:intergenic
ATCTGCGTACTCGCTTGCCT+GGG 0.511105 3:+15915468 MsaT011971.1:CDS
GTAATATATTGATGGCTGAT+TGG 0.511643 3:+15915988 MsaT011971.1:CDS
GATGCAGAGTCCTGATTCAA+TGG 0.511929 3:+15916466 MsaT011971.1:CDS
TAATGAATTTCTTAGGTGCA+AGG 0.518160 3:+15916334 MsaT011971.1:intron
AGCAGTGCCTGCGCATAAGA+AGG 0.521163 3:-15916548 None:intergenic
GTCGACATGGCGTAATGAAA+GGG 0.524194 3:-15916370 None:intergenic
AAAACAACTTTCTTGATGCA+TGG 0.533458 3:-15915906 None:intergenic
GCATACAACTTGAATCGTGC+TGG 0.535414 3:-15917251 None:intergenic
TGTCGACATGGCGTAATGAA+AGG 0.537636 3:-15916371 None:intergenic
CACATCTACAACCATTGCTA+AGG 0.537808 3:+15915724 MsaT011971.1:CDS
ATGAGTTGGTACTCACTGTT+CGG 0.540678 3:+15915964 MsaT011971.1:CDS
CATTGGTTGCTTCAAATCGG+TGG 0.542676 3:+15915653 MsaT011971.1:CDS
TTAGGTGCAAGGAATGGTGC+CGG 0.554256 3:+15916345 MsaT011971.1:intron
TAGGCCATTCGAGGCAGAGA+TGG 0.555199 3:+15915803 MsaT011971.1:CDS
CAAGTTGTATGCACTTCTGA+TGG 0.555433 3:+15917262 MsaT011971.1:CDS
CCCAATGCATTCTAGCAACA+AGG 0.557176 3:+15917082 MsaT011971.1:CDS
TACATTTAGCTGTTTGAGTG+CGG 0.561184 3:+15915510 MsaT011971.1:CDS
GTTACAACCTTCTTATGCGC+AGG 0.562566 3:+15916541 MsaT011971.1:CDS
GTCTACTGAATCCAAAACAA+TGG 0.568925 3:+15916033 MsaT011971.1:CDS
ACATAAACCTGCAATTGAGC+AGG 0.571518 3:-15915424 None:intergenic
GATGACAACATCATAATCCC+AGG 0.571751 3:-15915485 None:intergenic
AGAAGATATTCAGGAAACAA+TGG 0.583877 3:+15916133 MsaT011971.1:CDS
TGTCAATGGAAACACAATCA+AGG 0.590521 3:-15916403 None:intergenic
TCATGAAGGAACCTTAGCAA+TGG 0.594391 3:-15915735 None:intergenic
ATGGCGTAATGAAAGGGAGC+CGG 0.600819 3:-15916364 None:intergenic
TGCTAGAAGTTACAATGAGT+TGG 0.601484 3:+15915950 MsaT011971.1:CDS
ATACATGTCAAGAGCAACCC+TGG 0.604114 3:+15915342 None:intergenic
CAGGCAAGCGAGTACGCAGA+TGG 0.606937 3:-15915466 None:intergenic
CAGAGGTTGAAAGCTTTGCA+GGG 0.610511 3:+15916718 MsaT011971.1:CDS
AATTTCTTAGGTGCAAGGAA+TGG 0.610878 3:+15916339 MsaT011971.1:intron
ACAACTTTCTTGATGCATGG+TGG 0.612301 3:-15915903 None:intergenic
CATATTAAAGTTACACACGC+TGG 0.624968 3:+15916110 MsaT011971.1:CDS
ACAGCTAGGATATGTACACT+TGG 0.625444 3:-15916429 None:intergenic
AGCTGTTTGAGTGCGGAGTG+GGG 0.626137 3:+15915517 MsaT011971.1:CDS
ATGGTTGTAGATGTGTAAGT+TGG 0.627824 3:-15915716 None:intergenic
AGTAGACTTTCCACATGCGA+AGG 0.629550 3:-15916017 None:intergenic
TTAGCTGTTTGAGTGCGGAG+TGG 0.638306 3:+15915515 MsaT011971.1:CDS
AGAGTCCTGATTCAATGGCA+CGG 0.648352 3:+15916471 MsaT011971.1:CDS
TCATACAAGTTATCACAGCT+AGG 0.648492 3:-15916443 None:intergenic
TGTAACTCAATAAGATCTTG+GGG 0.649776 3:-15916524 None:intergenic
TTGGAATGATCCTTCGCATG+TGG 0.651252 3:+15916007 MsaT011971.1:CDS
AAACAATGGAAATTTCGTAG+TGG 0.666896 3:+15916047 MsaT011971.1:CDS
GCGGAATGACTTGTGTGCAA+AGG 0.667601 3:-15916805 None:intergenic
AGGCACAACATATGTCGACA+TGG 0.683756 3:-15916383 None:intergenic
AAGTTGCATATCTCGTCCAG+AGG 0.698811 3:+15916701 MsaT011971.1:CDS
ATTTGCTGGAACAACTACCA+GGG 0.699718 3:-15915359 None:intergenic
ACATTCCTGGAATGATGATG+CGG 0.709579 3:-15916824 None:intergenic
AAGTAGCATTAGTACCAATG+TGG 0.718006 3:+15916849 MsaT011971.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 15915346 15917281 15915346 ID=MsaG011971
Chr3 mRNA 15915346 15917281 15915346 ID=MsaT011971.1;Parent=MsaG011971
Chr3 exon 15915346 15915544 15915346 ID=MsaT011971.1.exon1;Parent=MsaT011971.1
Chr3 CDS 15915346 15915544 15915346 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Chr3 exon 15915637 15916159 15915637 ID=MsaT011971.1.exon2;Parent=MsaT011971.1
Chr3 CDS 15915637 15916159 15915637 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Chr3 exon 15916349 15916586 15916349 ID=MsaT011971.1.exon3;Parent=MsaT011971.1
Chr3 CDS 15916349 15916586 15916349 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Chr3 exon 15916661 15916933 15916661 ID=MsaT011971.1.exon4;Parent=MsaT011971.1
Chr3 CDS 15916661 15916933 15916661 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Chr3 exon 15917044 15917103 15917044 ID=MsaT011971.1.exon5;Parent=MsaT011971.1
Chr3 CDS 15917044 15917103 15917044 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Chr3 exon 15917231 15917281 15917231 ID=MsaT011971.1.exon6;Parent=MsaT011971.1
Chr3 CDS 15917231 15917281 15917231 ID=cds.MsaT011971.1;Parent=MsaT011971.1
Gene Sequence

>MsaT011971.1

ATGTCAAGAGCAACCCTGGTAGTTGTTCCAGCAAATTTGGTCGATCATTGGAAAGTACAAATAGAAAAGCATGTCAGGCCTGCTCAATTGCAGGTTTATGTTTGTAACGATCATCGGAAACCATCTGCGTACTCGCTTGCCTGGGATTATGATGTTGTCATCACTACATTTAGCTGTTTGAGTGCGGAGTGGGGCCCACTTGCAAATGACTATTTCATTGGTTGCTTCAAATCGGTGGATACTAACTGGAACACCAACACATCAAACACTCCTAACAGCCAACTTACACATCTACAACCATTGCTAAGGTTCCTTCATGAAGAAGCTTATGGACTGAATCAAAAATCATGGGATGCTGGCGTGCTTAGGCCATTCGAGGCAGAGATGGAAAAAGGTTGGTCTCGTCTGTTGCGTTTACTTCAGAAATGCATGATTAGTGCTAGAAAGGCAGATTTGCAAAGTATTCCACCATGCATCAAGAAAGTTGTTTTACTAGATTTTAATGAGGAACATGCTAGAAGTTACAATGAGTTGGTACTCACTGTTCGGCGTAATATATTGATGGCTGATTGGAATGATCCTTCGCATGTGGAAAGTCTACTGAATCCAAAACAATGGAAATTTCGTAGTGGAGCTATAAAAAATGTAAGGCTTTCATGCTGTGTTGCTGGACATATTAAAGTTACACACGCTGGAGAAGATATTCAGGAAACAATGGATATGTGCAAGGAATGGTGCCGGCTCCCTTTCATTACGCCATGTCGACATATGTTGTGCCTTGATTGTGTTTCCATTGACAAGACCAAGTGTACATATCCTAGCTGTGATAACTTGTATGAGATGCAGAGTCCTGATTCAATGGCACGGCCAGAAAATCCTAACCTAAAGTGGTTTGTACCCCAAGATCTTATTGAGTTACAACCTTCTTATGCGCAGGCACTGCTTTTAATTGAGAAATCAGATAATTGGGATCCTGATTGGCAATCAACATCTAGTAGTAAAGTTGCATATCTCGTCCAGAGGTTGAAAGCTTTGCAGGGAACCAATGAAGAGATGAGTTCATATACTGACAATAGCAATGTGATGTATATTGAAAATAGTTTTCCTTTGCACACAAGTCATTCCGCATCATCATTCCAGGAATGTTCAAGTAGCATTAGTACCAATGTGGTCCCTGAAAAAGTTTTGATATTTTCTCAGTTTCTTGAGCATATACATGTCATTGAACAACAGTTGGCTGTTAATGGTATCAAATATACTGGAATGTATAACCCAATGCATTCTAGCAACAAGAAGAAGTCATTAGCCAAGTTCCAGCACGATTCAAGTTGTATGCACTTCTGA

Protein sequence

>MsaT011971.1

MSRATLVVVPANLVDHWKVQIEKHVRPAQLQVYVCNDHRKPSAYSLAWDYDVVITTFSCLSAEWGPLANDYFIGCFKSVDTNWNTNTSNTPNSQLTHLQPLLRFLHEEAYGLNQKSWDAGVLRPFEAEMEKGWSRLLRLLQKCMISARKADLQSIPPCIKKVVLLDFNEEHARSYNELVLTVRRNILMADWNDPSHVESLLNPKQWKFRSGAIKNVRLSCCVAGHIKVTHAGEDIQETMDMCKEWCRLPFITPCRHMLCLDCVSIDKTKCTYPSCDNLYEMQSPDSMARPENPNLKWFVPQDLIELQPSYAQALLLIEKSDNWDPDWQSTSSSKVAYLVQRLKALQGTNEEMSSYTDNSNVMYIENSFPLHTSHSASSFQECSSSISTNVVPEKVLIFSQFLEHIHVIEQQLAVNGIKYTGMYNPMHSSNKKKSLAKFQHDSSCMHF*