AlfalfaGEDB | MsaG012006

M. sativa ssp. caerulea / MsaG012006

KEGG: -
GO: -
PFAM: PF05142.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012006	XP_003598516.1	87.407	270	11	4	1	256	69	329	1.73e-150	433

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012006	sp\|Q94CK9\|LRP1_ARATH	48.000	200	84	4	69	251	112	308	4.04e-49	167

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012006	tr\|G7J0K6\|G7J0K6_MEDTR	87.407	270	11	4	1	256	69	329	8.24e-151	433

TFs/TRs:

Gene ID	Type	Classification
MsaG012006	TF	SRS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000371	MsaG012006	0.864605	2.150629e-64	2.000103e-61
MsaG007404	MsaG012006	0.873110	3.896389e-67	5.158535e-64
MsaG007406	MsaG012006	0.874687	1.149568e-67	1.630594e-64
MsaG008436	MsaG012006	0.832381	1.766358e-55	5.475968e-53
MsaG008863	MsaG012006	0.808493	5.134094e-50	8.454793e-48
MsaG010187	MsaG012006	0.805045	2.726770e-49	4.138328e-47
MsaG010191	MsaG012006	0.908571	3.545446e-81	3.056354e-77
MsaG010966	MsaG012006	0.854777	1.896084e-61	1.215170e-58
MsaG012005	MsaG012006	0.855899	8.972056e-62	5.987758e-59
MsaG012006	MsaG016508	0.863741	3.987046e-64	3.582764e-61
MsaG012006	MsaG021237	0.842811	3.833208e-58	1.635981e-55
MsaG012006	MsaG023394	0.878077	7.873214e-69	1.300469e-65
MsaG012006	MsaG023544	0.805395	2.304808e-49	3.526417e-47
MsaG012006	MsaG023596	0.949084	8.265246e-107	1.725850e-101
MsaG012006	MsaG023597	0.885151	2.242346e-71	5.174569e-68
MsaG012006	MsaG023600	0.894788	3.980982e-75	1.515838e-71
MsaG012006	MsaG026693	0.891695	6.957355e-74	2.240897e-70
MsaG012006	MsaG026729	0.954647	6.215558e-112	2.037068e-106
MsaG012006	MsaG026728	0.916700	3.259644e-85	4.842237e-81
MsaG012006	MsaG026730	0.887051	4.348654e-72	1.102208e-68
MsaG012006	MsaG027034	0.821219	7.968757e-53	1.810095e-50
MsaG012006	MsaG030055	0.873623	2.625125e-67	3.553317e-64
MsaG012006	MsaG030680	0.851855	1.293644e-60	7.476620e-58
MsaG012006	MsaG046085	0.923431	6.997078e-89	1.701634e-84
MsaG012006	MsaG002985	0.801948	1.187089e-48	1.677347e-46
MsaG012006	MsaG008731	0.847956	1.574755e-59	7.963442e-57
MsaG012006	MsaG027221	0.934545	9.644536e-96	5.700420e-91
MsaG012006	MsaG026405	0.909857	8.644073e-82	8.085778e-78
MsaG012006	MsaG030006	0.844461	1.395068e-58	6.279796e-56
MsaG012006	MsaG037887	0.836260	1.898413e-56	6.604335e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012006	MtrunA17_Chr3g0082511	87.407	270	11	4	1	256	69	329	1.59e-154	433
MsaG012006	MtrunA17_Chr8g0353111	57.471	261	74	10	22	255	76	326	3.30e-88	265
MsaG012006	MtrunA17_Chr5g0441921	52.071	169	71	2	69	228	124	291	7.56e-49	164
MsaG012006	MtrunA17_Chr8g0372461	42.466	146	67	2	69	213	121	250	1.98e-35	129
MsaG012006	MtrunA17_Chr1g0155791	40.260	154	91	1	69	221	122	275	2.43e-32	120
MsaG012006	MtrunA17_Chr5g0404781	43.506	154	80	2	69	215	100	253	5.69e-31	117
MsaG012006	MtrunA17_Chr3g0142171	43.709	151	69	6	69	213	86	226	4.43e-28	108
MsaG012006	MtrunA17_Chr4g0035591	37.415	147	68	3	69	215	20	142	1.23e-25	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012006	AT5G12330.1	48.000	200	84	4	69	251	112	308	4.11e-50	167
MsaG012006	AT5G12330.2	48.000	200	84	4	69	251	112	308	4.11e-50	167
MsaG012006	AT5G12330.4	48.000	200	84	4	69	251	199	395	2.27e-49	167
MsaG012006	AT3G54430.1	47.059	170	71	4	57	225	31	182	3.92e-44	147
MsaG012006	AT5G66350.3	46.575	146	72	2	69	213	120	260	9.03e-39	138
MsaG012006	AT5G66350.2	46.575	146	72	2	69	213	120	260	9.96e-39	138
MsaG012006	AT5G66350.1	46.575	146	72	2	69	213	120	260	1.59e-38	137
MsaG012006	AT3G51060.1	47.222	144	70	4	69	211	144	282	4.13e-36	132
MsaG012006	AT1G19790.2	39.645	169	60	3	69	213	119	269	2.26e-34	127
MsaG012006	AT1G19790.1	39.645	169	60	3	69	213	119	269	2.26e-34	127
MsaG012006	AT1G75520.1	41.139	158	80	4	69	213	125	282	1.28e-33	125
MsaG012006	AT4G36260.1	38.914	221	118	5	8	213	26	244	2.28e-32	121
MsaG012006	AT2G21400.2	37.162	148	78	2	67	214	8	140	7.90e-31	113
MsaG012006	AT2G21400.4	37.162	148	78	2	67	214	7	139	8.65e-31	113
MsaG012006	AT2G18120.2	41.060	151	71	3	69	217	72	206	1.48e-30	114
MsaG012006	AT2G18120.1	40.132	152	73	2	69	217	72	208	4.21e-30	112
MsaG012006	AT2G21400.3	37.838	148	62	2	67	214	8	125	1.98e-29	109
MsaG012006	AT2G21400.1	37.838	148	62	2	67	214	7	124	2.24e-29	109
MsaG012006	AT5G12330.3	51.613	93	40	2	69	159	112	201	3.69e-21	89.0
MsaG012006	AT5G33210.1	37.179	156	87	5	38	192	20	165	5.16e-20	84.7
MsaG012006	AT5G33210.2	65.306	49	17	0	69	117	17	65	5.26e-19	80.1

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGGGTAAACATTATTATT+TGG	0.173024	3:-16721300	None:intergenic
TGAAGATGAGTTTGCCTATT+TGG	0.237838	3:+16721128	MsaT012006.1:CDS
TTTGTCAAGATTGTGGTAAT+AGG	0.301923	3:+16719822	MsaT012006.1:CDS
TCTGGTGTTAAGAGACAAAA+AGG	0.305575	3:+16720019	MsaT012006.1:CDS
AATATGAACTGTGGCCAAAT+AGG	0.325865	3:-16721142	None:intergenic
TTTGGAAGATTCACTACTTC+AGG	0.330560	3:-16719710	None:intergenic
TTCATGCACCAGCTGTATTC+AGG	0.332427	3:+16721076	MsaT012006.1:CDS
CTCTATGATCATGGTGTTGA+TGG	0.354571	3:+16721189	MsaT012006.1:CDS
GGTGATGGTGAAGGTTGTTC+TGG	0.358144	3:+16720001	MsaT012006.1:CDS
TTGTTGTTACTATTATTGTT+AGG	0.359102	3:-16719665	None:intergenic
CGGGAGGTGGAGATGTTTGC+CGG	0.390512	3:+16719956	MsaT012006.1:CDS
TCTTGAACTTCATTGGTTTG+AGG	0.394602	3:-16719638	None:intergenic
TTGGCCACAGTTCATATTAG+TGG	0.395130	3:+16721147	MsaT012006.1:CDS
TGCCAATAGCAGTTACTCTA+TGG	0.398017	3:-16721101	None:intergenic
GTGGAGATGTTTGCCGGTGG+TGG	0.398827	3:+16719962	MsaT012006.1:CDS
TTGTCAAGATTGTGGTAATA+GGG	0.399150	3:+16719823	MsaT012006.1:CDS
GAGATGTTTGCCGGTGGTGG+TGG	0.399774	3:+16719965	MsaT012006.1:CDS
ATGTTTGCCGGTGGTGGTGG+TGG	0.402912	3:+16719968	MsaT012006.1:CDS
TTGTGTTTCAGAACTTCAAT+TGG	0.409173	3:+16721227	MsaT012006.1:CDS
TTCGACACGTCGTCGGGAAC+GGG	0.417355	3:+16719937	MsaT012006.1:CDS
TTTGCCGGTGGTGGTGGTGG+TGG	0.424345	3:+16719971	MsaT012006.1:CDS
GTACTCATTTGAAGAGTACT+TGG	0.429685	3:+16719909	MsaT012006.1:CDS
GGTGGTGGTGGTGATGGTGA+AGG	0.434194	3:+16719992	MsaT012006.1:CDS
AAGATGTAGGACTTGTTGTA+AGG	0.436244	3:+16719868	MsaT012006.1:CDS
AAAGGGTTTCTCTATGATCA+TGG	0.441459	3:+16721180	MsaT012006.1:CDS
CATGGCTAGCTAGCAGAAGC+AGG	0.449543	3:-16721318	None:intergenic
TGCCATAGAGTAACTGCTAT+TGG	0.452645	3:+16721099	MsaT012006.1:CDS
GGTGAATGTTCTTCTGCAAT+TGG	0.456180	3:+16721273	MsaT012006.1:CDS
GGTTTCAAAGAGGGTTTACC+AGG	0.457703	3:+16721048	MsaT012006.1:CDS
TCTCATAGCTCTAACTCTAA+TGG	0.458294	3:+16720076	MsaT012006.1:CDS
TCATGGCAAGATGGCTATGA+TGG	0.459692	3:+16719760	MsaT012006.1:CDS
GATTCCTTCGACACGTCGTC+GGG	0.466088	3:+16719931	MsaT012006.1:CDS
ACGTCGTCGGGAACGGGAGG+TGG	0.470316	3:+16719943	MsaT012006.1:CDS
AGTTCTGAAACACAAGGCAT+TGG	0.476027	3:-16721219	None:intergenic
AGTGAAGAAAATGGTGTGTA+TGG	0.483753	3:+16719785	MsaT012006.1:CDS
TGTGTTTCAGAACTTCAATT+GGG	0.498389	3:+16721228	MsaT012006.1:CDS
CTACTTCAGGACACATCCTA+AGG	0.507824	3:-16719697	None:intergenic
GTTTGAGGTTGTTGTTGTTG+TGG	0.509148	3:-16719623	None:intergenic
CTTCGACACGTCGTCGGGAA+CGG	0.511061	3:+16719936	MsaT012006.1:CDS
TATAGAGTTTGTCAAGATTG+TGG	0.512497	3:+16719815	MsaT012006.1:CDS
AATAATTGTAGTGGAAAGAA+TGG	0.512653	3:+16721252	MsaT012006.1:CDS
ATGATGGAGAGTGAAGAAAA+TGG	0.542008	3:+16719776	MsaT012006.1:CDS
GGATTCCTTCGACACGTCGT+CGG	0.554911	3:+16719930	MsaT012006.1:CDS
GCAGATGCAGGTTTCAAAGA+GGG	0.558109	3:+16721039	MsaT012006.1:intron
CTATGGCACCTGAATACAGC+TGG	0.563710	3:-16721084	None:intergenic
ATCACTGAAGAAGAGAATCA+TGG	0.572196	3:+16719743	MsaT012006.1:CDS
AGAGTAACTGCTATTGGCAA+TGG	0.579028	3:+16721105	MsaT012006.1:CDS
CAATTGGGAAATAATTGTAG+TGG	0.580003	3:+16721243	MsaT012006.1:CDS
AGATGTAGGACTTGTTGTAA+GGG	0.582676	3:+16719869	MsaT012006.1:CDS
AGAAGAGAATCATGGCAAGA+TGG	0.584436	3:+16719751	MsaT012006.1:CDS
CGTTCCCGACGACGTGTCGA+AGG	0.590725	3:-16719935	None:intergenic
AGAGAAACCCTTTGAAAACA+TGG	0.595856	3:-16721170	None:intergenic
ATTGTGTGTTTAGAAGATGT+AGG	0.602227	3:+16719855	MsaT012006.1:CDS
TCTATGATCATGGTGTTGAT+GGG	0.608318	3:+16721190	MsaT012006.1:CDS
TGCAGATGCAGGTTTCAAAG+AGG	0.617800	3:+16721038	MsaT012006.1:intron
GACACGTCGTCGGGAACGGG+AGG	0.631647	3:+16719940	MsaT012006.1:CDS
AGGACTTGTTGTAAGGGACG+TGG	0.632558	3:+16719875	MsaT012006.1:CDS
ATGGCTAGCTAGCAGAAGCA+GGG	0.656680	3:-16721317	None:intergenic
GAGGTGGAGATGTTTGCCGG+TGG	0.687056	3:+16719959	MsaT012006.1:CDS
GCTGGTGCATGAACATGACC+TGG	0.691114	3:-16721066	None:intergenic
ATGGCCACTAATATGAACTG+TGG	0.704516	3:-16721151	None:intergenic
AATTGAAGTTCTGAAACACA+AGG	0.729820	3:-16721225	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	16719620	16721335	16719620	ID=MsaG012006
Chr3	mRNA	16719620	16721335	16719620	ID=MsaT012006.1;Parent=MsaG012006
Chr3	exon	16719620	16720097	16719620	ID=MsaT012006.1.exon1;Parent=MsaT012006.1
Chr3	CDS	16719620	16720097	16719620	ID=cds.MsaT012006.1;Parent=MsaT012006.1
Chr3	exon	16721043	16721335	16721043	ID=MsaT012006.1.exon2;Parent=MsaT012006.1
Chr3	CDS	16721043	16721335	16721043	ID=cds.MsaT012006.1;Parent=MsaT012006.1

Gene Sequence

>MsaT012006.1

ATGCCACAACAACAACAACCTCAAACCAATGAAGTTCAAGAAAATCCTAACAATAATAGTAACAACAATTTTTGGAACCTTAGGATGTGTCCTGAAGTAGTGAATCTTCCAAAAAAAGGAGTGATCACTGAAGAAGAGAATCATGGCAAGATGGCTATGATGGAGAGTGAAGAAAATGGTGTGTATGGTTCTGAGTATAGAGTTTGTCAAGATTGTGGTAATAGGGCTAAGAAAGATTGTGTGTTTAGAAGATGTAGGACTTGTTGTAAGGGACGTGGTTATGATTGTAGTACTCATTTGAAGAGTACTTGGATTCCTTCGACACGTCGTCGGGAACGGGAGGTGGAGATGTTTGCCGGTGGTGGTGGTGGTGGTGGTGGTGGTGATGGTGAAGGTTGTTCTGGTGTTAAGAGACAAAAAGGTTTATTAGGGTCATCACAAAATGCTGCAGCTACTTCTCATAGCTCTAACTCTAATGATGCAGGTTTCAAAGAGGGTTTACCAGGTCATGTTCATGCACCAGCTGTATTCAGGTGCCATAGAGTAACTGCTATTGGCAATGGTGAAGATGAGTTTGCCTATTTGGCCACAGTTCATATTAGTGGCCATGTTTTCAAAGGGTTTCTCTATGATCATGGTGTTGATGGGAAAAATCCAATGCCTTGTGTTTCAGAACTTCAATTGGGAAATAATTGTAGTGGAAAGAATGGTGAATGTTCTTCTGCAATTGGTGTTCCAAATAATAATGTTTACCCTGCTTCTGCTAGCTAG

Protein sequence

>MsaT012006.1

MPQQQQPQTNEVQENPNNNSNNNFWNLRMCPEVVNLPKKGVITEEENHGKMAMMESEENGVYGSEYRVCQDCGNRAKKDCVFRRCRTCCKGRGYDCSTHLKSTWIPSTRRREREVEMFAGGGGGGGGGDGEGCSGVKRQKGLLGSSQNAAATSHSSNSNDAGFKEGLPGHVHAPAVFRCHRVTAIGNGEDEFAYLATVHISGHVFKGFLYDHGVDGKNPMPCVSELQLGNNCSGKNGECSSAIGVPNNNVYPASAS*