AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012133

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012133 MtrunA17_Chr3g0080761 95.789 665 21 4 1 659 1 664 0.0 1317
MsaG012133 MtrunA17_Chr8g0354821 59.652 689 228 9 15 658 4 687 0.0 840
MsaG012133 MtrunA17_Chr7g0255181 46.794 733 286 18 1 658 1 704 0.0 632
MsaG012133 MtrunA17_Chr5g0399801 41.527 537 292 13 125 656 567 1086 3.66e-122 389
MsaG012133 MtrunA17_Chr4g0003501 40.301 531 292 15 133 658 444 954 3.36e-114 365
MsaG012133 MtrunA17_Chr4g0003511 39.703 539 302 14 125 658 531 1051 2.71e-112 362
MsaG012133 MtrunA17_Chr8g0368901 41.239 468 255 12 174 631 299 756 1.21e-109 348
MsaG012133 MtrunA17_Chr8g0368911 40.546 476 251 12 172 631 290 749 1.51e-109 348
MsaG012133 MtrunA17_Chr1g0163051 37.009 535 302 14 108 631 135 645 1.98e-106 337
MsaG012133 MtrunA17_Chr8g0369301 40.179 448 247 9 194 631 300 736 3.73e-104 333
MsaG012133 MtrunA17_Chr6g0476111 36.986 511 281 14 171 655 195 690 6.68e-97 312
MsaG012133 MtrunA17_Chr6g0476121 36.204 511 285 14 171 655 144 639 2.65e-94 304
MsaG012133 MtrunA17_Chr7g0252211 36.759 506 294 11 169 655 255 753 1.29e-92 303
MsaG012133 MtrunA17_Chr1g0163041 34.570 512 301 14 139 631 68 564 5.81e-91 293
MsaG012133 MtrunA17_Chr6g0467381 35.115 524 289 13 139 631 60 563 1.36e-89 290
MsaG012133 MtrunA17_Chr6g0467531 34.981 526 291 13 139 633 60 565 3.70e-89 288
MsaG012133 MtrunA17_Chr6g0467421 34.924 524 290 13 139 631 60 563 6.81e-89 288
MsaG012133 MtrunA17_Chr6g0467401 34.791 526 292 13 139 633 60 565 1.51e-87 284
MsaG012133 MtrunA17_Chr3g0094881 35.098 510 291 14 135 633 144 624 7.44e-84 276
MsaG012133 MtrunA17_Chr6g0467411 32.994 491 279 13 155 633 31 483 2.79e-71 239
MsaG012133 MtrunA17_Chr2g0306961 31.641 512 277 11 139 633 9 464 3.76e-70 235
MsaG012133 MtrunA17_Chr1g0170651 33.607 488 266 12 189 658 98 545 5.07e-66 227
MsaG012133 MtrunA17_Chr7g0232811 29.670 546 272 18 135 654 75 534 7.23e-61 212
MsaG012133 MtrunA17_Chr7g0232981 29.301 529 273 18 135 654 75 511 3.16e-54 194
MsaG012133 MtrunA17_Chr7g0232841 28.885 547 261 21 135 654 78 523 6.48e-52 187
MsaG012133 MtrunA17_Chr5g0403241 31.562 320 168 9 370 658 1207 1506 1.57e-32 135
MsaG012133 MtrunA17_Chr1g0190311 27.811 338 168 11 372 655 325 640 9.56e-31 128
MsaG012133 MtrunA17_Chr8g0373821 29.193 322 177 9 359 654 237 533 5.98e-29 121
MsaG012133 MtrunA17_Chr1g0183321 28.743 334 169 10 372 655 428 742 3.65e-28 120
MsaG012133 MtrunA17_Chr4g0075321 34.703 219 114 5 445 658 869 1063 6.90e-28 120
MsaG012133 MtrunA17_Chr7g0262001 28.049 328 173 7 374 655 388 698 1.54e-25 112
MsaG012133 MtrunA17_Chr7g0232751 37.963 108 63 2 549 654 9 114 4.50e-16 74.7
MsaG012133 MtrunA17_Chr2g0305371 30.928 194 96 5 463 656 314 469 2.98e-15 79.0
MsaG012133 MtrunA17_Chr7g0232761 32.867 143 77 6 192 329 141 269 8.00e-12 66.2
MsaG012133 MtrunA17_Chr7g0232871 38.667 75 39 2 477 547 16 87 2.98e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012133 AT5G04940.2 56.250 592 236 8 70 658 98 669 0.0 683
MsaG012133 AT5G04940.1 56.250 592 236 8 70 658 98 669 0.0 683
MsaG012133 AT1G73100.1 51.180 678 291 10 6 658 6 668 0.0 682
MsaG012133 AT1G17770.1 43.351 579 292 12 98 659 134 693 9.39e-154 460
MsaG012133 AT2G24740.1 44.732 541 250 14 129 658 252 754 9.63e-147 444
MsaG012133 AT2G35160.5 38.031 589 306 19 80 658 218 757 1.30e-111 353
MsaG012133 AT2G35160.2 38.031 589 306 19 80 658 254 793 5.60e-111 352
MsaG012133 AT2G35160.3 38.031 589 306 19 80 658 254 793 5.60e-111 352
MsaG012133 AT2G35160.1 38.031 589 306 19 80 658 254 793 5.60e-111 352
MsaG012133 AT2G35160.4 38.031 589 306 19 80 658 254 793 5.60e-111 352
MsaG012133 AT4G13460.1 38.614 505 286 11 139 631 145 637 4.25e-107 338
MsaG012133 AT4G13460.2 38.614 505 286 11 139 631 145 637 4.25e-107 338
MsaG012133 AT2G22740.2 37.782 532 301 13 139 655 270 786 5.79e-106 339
MsaG012133 AT2G22740.1 37.782 532 301 13 139 655 270 786 5.79e-106 339
MsaG012133 AT2G33290.1 38.780 508 297 8 139 640 149 648 8.78e-106 335
MsaG012133 AT2G33290.3 38.780 508 297 8 139 640 149 648 8.78e-106 335
MsaG012133 AT2G33290.4 38.780 508 297 8 139 640 149 648 8.78e-106 335
MsaG012133 AT2G33290.2 38.780 508 297 8 139 640 149 648 8.78e-106 335
MsaG012133 AT5G13960.2 36.420 486 276 14 192 655 12 486 1.34e-91 293
MsaG012133 AT5G13960.1 35.490 510 296 14 168 655 122 620 1.14e-90 295
MsaG012133 AT2G05900.1 41.176 221 121 4 212 428 1 216 5.13e-45 163
MsaG012133 AT2G05900.1 56.098 82 30 1 577 658 236 311 2.09e-22 99.0
MsaG012133 AT5G47150.1 41.589 214 113 5 139 347 118 324 2.49e-41 153
MsaG012133 AT3G04380.2 28.012 332 174 8 372 655 117 431 3.94e-36 142
MsaG012133 AT3G04380.1 28.012 332 174 8 372 655 144 458 4.51e-36 142
MsaG012133 AT3G04380.3 28.012 332 174 8 372 655 128 442 5.01e-36 142
MsaG012133 AT3G04380.6 28.012 332 174 8 372 655 117 431 5.26e-36 141
MsaG012133 AT3G04380.4 28.012 332 174 8 372 655 144 458 5.38e-36 142
MsaG012133 AT3G04380.5 28.012 332 174 8 372 655 101 415 5.40e-36 141
MsaG012133 AT5G47160.1 37.549 253 137 8 103 344 166 408 1.81e-34 136
MsaG012133 AT5G47160.3 37.549 253 137 8 103 344 166 408 1.81e-34 136
MsaG012133 AT5G47160.2 37.549 253 137 8 103 344 166 408 1.81e-34 136
MsaG012133 AT2G23740.1 32.288 319 166 15 368 659 1080 1375 1.94e-32 135
MsaG012133 AT2G23740.3 32.288 319 166 15 368 659 1087 1382 1.98e-32 135
MsaG012133 AT2G23740.6 32.288 319 166 15 368 659 1087 1382 1.98e-32 135
MsaG012133 AT2G23740.2 32.288 319 166 15 368 659 1087 1382 1.98e-32 135
MsaG012133 AT1G04050.3 30.539 334 161 13 372 655 303 615 1.15e-31 131
MsaG012133 AT1G04050.2 31.138 334 159 14 372 655 407 719 1.49e-31 131
MsaG012133 AT1G04050.1 31.138 334 159 14 372 655 407 719 1.49e-31 131
MsaG012133 AT5G43990.7 28.144 334 166 12 372 655 304 613 1.45e-28 121
MsaG012133 AT5G43990.3 28.144 334 166 12 372 655 393 702 1.55e-28 122
MsaG012133 AT5G43990.11 28.144 334 166 12 372 655 393 702 1.55e-28 122
MsaG012133 AT5G43990.1 28.144 334 166 12 372 655 393 702 1.55e-28 122
MsaG012133 AT5G43990.4 28.144 334 166 12 372 655 393 702 1.55e-28 122
MsaG012133 AT5G43990.15 28.144 334 166 12 372 655 396 705 1.62e-28 122
MsaG012133 AT5G43990.10 28.144 334 166 12 372 655 396 705 1.62e-28 122
MsaG012133 AT5G43990.9 28.144 334 166 12 372 655 416 725 1.67e-28 122
MsaG012133 AT5G43990.2 28.144 334 166 12 372 655 416 725 1.67e-28 122
MsaG012133 AT5G43990.16 28.144 334 166 12 372 655 373 682 1.71e-28 121
MsaG012133 AT5G43990.5 28.144 334 166 12 372 655 373 682 1.71e-28 121
MsaG012133 AT5G43990.17 28.144 334 166 12 372 655 373 682 1.71e-28 121
MsaG012133 AT5G43990.14 28.144 334 166 12 372 655 373 682 1.71e-28 121
MsaG012133 AT5G43990.8 28.144 334 166 12 372 655 324 633 2.00e-28 121
MsaG012133 AT5G43990.12 28.144 334 166 12 372 655 324 633 2.00e-28 121
MsaG012133 AT5G43990.6 28.144 334 166 12 372 655 324 633 2.00e-28 121
MsaG012133 AT5G43990.13 28.144 334 166 12 372 655 324 633 2.00e-28 121
MsaG012133 AT3G03750.2 33.186 226 128 7 435 658 145 349 7.25e-25 107
MsaG012133 AT2G23740.4 32.530 249 120 14 368 589 1087 1314 1.10e-20 97.8
MsaG012133 AT2G23740.5 32.530 249 120 14 368 589 1087 1314 1.10e-20 97.8
MsaG012133 AT3G03750.1 29.911 224 122 7 435 658 145 333 1.99e-18 87.8
MsaG012133 AT1G76710.2 32.020 203 88 7 462 656 66 226 2.02e-16 83.2
MsaG012133 AT1G76710.1 32.020 203 88 7 462 656 66 226 2.02e-16 83.2
MsaG012133 AT1G77300.2 30.890 191 98 5 466 656 1008 1164 2.06e-16 84.3
MsaG012133 AT1G76710.3 32.020 203 88 7 462 656 66 226 2.86e-16 82.8
MsaG012133 AT1G77300.1 30.890 191 98 5 466 656 1008 1164 2.87e-16 84.0
MsaG012133 AT1G76710.4 32.020 203 88 7 462 656 66 226 3.07e-16 82.4
MsaG012133 AT4G30860.1 30.256 195 96 6 463 656 307 462 6.61e-14 75.1
MsaG012133 AT3G59960.3 28.333 180 75 6 485 655 112 246 5.55e-12 67.4
MsaG012133 AT3G59960.2 28.333 180 75 6 485 655 112 246 5.55e-12 67.4
MsaG012133 AT3G59960.4 28.333 180 75 6 485 655 112 246 5.55e-12 67.4
MsaG012133 AT3G59960.1 28.333 180 75 6 485 655 112 246 1.76e-11 66.6

Find 164 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAGGTCAGTATCCTTC+TGG 0.204050 3:+19588214 MsaT012133.1:CDS
ACAGGTATCAAACTTCTTAT+AGG 0.235001 3:-19588166 None:intergenic
CGACTCCACTTCAAGCATTT+AGG 0.258428 3:+19588305 MsaT012133.1:CDS
CAAGCATTTAGGAGCCCTTT+TGG 0.274971 3:+19588316 MsaT012133.1:CDS
CATTGACAAGTATAAGATTC+TGG 0.293840 3:+19588135 MsaT012133.1:CDS
AGAGTGGAAATATGTGAAAA+AGG 0.306165 3:-19589280 None:intergenic
TGCATTAAACTGAATGATTT+CGG 0.306178 3:-19589312 None:intergenic
CATGAGTTAACATATGATTA+TGG 0.312052 3:+19589960 MsaT012133.1:CDS
TTATATTATCAATGGAGTTC+TGG 0.315928 3:+19589428 MsaT012133.1:CDS
AGAACTCCATTGATAATATA+AGG 0.322628 3:-19589426 None:intergenic
TGTATAAAATTCAAGATTCA+TGG 0.343226 3:+19589046 MsaT012133.1:CDS
TTCTTAGCATTTATAATAAG+AGG 0.346128 3:-19589804 None:intergenic
AGCATGTGTCCCTGGCGATT+TGG 0.346957 3:+19589368 MsaT012133.1:CDS
CGAATGGAAATGTGTGTTAT+CGG 0.357207 3:+19588715 MsaT012133.1:CDS
CTTCAGGTCAGTATCCTTCT+GGG 0.359790 3:+19588215 MsaT012133.1:CDS
AACCTAGTGCCTTTGCCGTT+TGG 0.360454 3:+19589130 MsaT012133.1:CDS
TTTGGTGCTTCAAACCGGTT+TGG 0.365259 3:-19589524 None:intergenic
TCATAAGGGTAATCTTGCTT+TGG 0.385063 3:+19588930 MsaT012133.1:CDS
CAAAACAAAGGATAAAGGTT+GGG 0.388648 3:+19589557 MsaT012133.1:CDS
TTGGTGCTTCAAACCGGTTT+GGG 0.391461 3:-19589523 None:intergenic
AATCCTCAAGGTCAATCTTC+AGG 0.392097 3:+19588199 MsaT012133.1:CDS
TGTTATAATTTATACCGGTC+AGG 0.396575 3:+19588861 MsaT012133.1:CDS
ACTTCATTGACAAGTGATAC+TGG 0.399879 3:-19589237 None:intergenic
TGTGTTTGTTCGAATTCCTT+TGG 0.402301 3:-19588638 None:intergenic
GATTGATGCCTTGCATATCC+AGG 0.403598 3:+19588762 MsaT012133.1:CDS
ACTTGAAAGATTCATAAATA+CGG 0.407062 3:-19589710 None:intergenic
CGAACCTGTGTTCCATTCCT+TGG 0.409287 3:-19588575 None:intergenic
CGGTTTGCATGCTCAATCAA+TGG 0.409404 3:+19588735 MsaT012133.1:CDS
CAGTACCTGGAGTTGAGATT+GGG 0.418871 3:+19588677 MsaT012133.1:CDS
ACAGGCTGCCAGAAAACATT+TGG 0.419329 3:-19589867 None:intergenic
ATTTGGACTGCTCATGCATT+CGG 0.422721 3:+19589385 MsaT012133.1:CDS
TACCATTGTCTCTTTGACCT+GGG 0.423334 3:-19588480 None:intergenic
TCAGTACCTGGAGTTGAGAT+TGG 0.424381 3:+19588676 MsaT012133.1:CDS
TTCCATTTGGTGCTTCAAAC+CGG 0.425045 3:-19589529 None:intergenic
ATTATAAATGCTAAGAATGT+TGG 0.437154 3:+19589810 MsaT012133.1:CDS
GGTTTGCATGCTCAATCAAT+GGG 0.437843 3:+19588736 MsaT012133.1:CDS
GCTGTTAGCATTGTTTCGTC+AGG 0.439867 3:+19588808 MsaT012133.1:CDS
AGAATAAGTCCAGTCCTTGC+AGG 0.449322 3:-19589183 None:intergenic
AAGGTTGGGGTCTTCGATCA+TGG 0.452431 3:+19589571 MsaT012133.1:CDS
GCTGGTTTCCCTGCAAGGAC+TGG 0.456055 3:+19589174 MsaT012133.1:CDS
ATAAAAGCACCAGCACGAAT+AGG 0.460396 3:-19589597 None:intergenic
TTACCATTGTCTCTTTGACC+TGG 0.461172 3:-19588481 None:intergenic
GTGTTCAAAACAAAGGATAA+AGG 0.462890 3:+19589552 MsaT012133.1:CDS
GTGAATTGTATAATGGAAGA+AGG 0.464015 3:+19588079 None:intergenic
CGATAACACACATTTCCATT+CGG 0.476797 3:-19588714 None:intergenic
TATTCTTGCTGACCTCTCCT+CGG 0.477270 3:+19589200 MsaT012133.1:CDS
AGATCCTCATCCTCTCCAAA+AGG 0.481832 3:-19588331 None:intergenic
GTATCATGAGGTCCACCAAA+TGG 0.483316 3:-19588253 None:intergenic
CTTGAAAGATTCATAAATAC+GGG 0.486596 3:-19589709 None:intergenic
GTCAGATCAGAAGCTTCATA+AGG 0.487005 3:+19588915 MsaT012133.1:CDS
CATGAGCAGTCCAAATCGCC+AGG 0.488074 3:-19589378 None:intergenic
GTTGATATGAGTGGTTTAGT+TGG 0.492160 3:+19588451 MsaT012133.1:CDS
TGTATAATGGAAGAAGGGTT+AGG 0.492317 3:+19588085 None:intergenic
CAGAATCTTATACTTGTCAA+TGG 0.495015 3:-19588134 None:intergenic
GCACAATATTAACAACTTCA+CGG 0.500326 3:-19588504 None:intergenic
TGCATATCCAGGGTGACCGC+GGG 0.500357 3:+19588773 MsaT012133.1:CDS
TCACAGCATCTTTGATGCCT+CGG 0.503981 3:-19588990 None:intergenic
TATCATGAGGTCCACCAAAT+GGG 0.505449 3:-19588252 None:intergenic
TTGTTCTCTTCGTATAAGAC+AGG 0.505552 3:-19589885 None:intergenic
AACCGGTTTGAAGCACCAAA+TGG 0.508329 3:+19589527 MsaT012133.1:CDS
TCTGTTCAGAAGTGGAAAGC+TGG 0.508734 3:+19589156 MsaT012133.1:CDS
ATCAAAGATGCTGTGACTCC+AGG 0.511194 3:+19588997 MsaT012133.1:CDS
GGTGATGTTATAATTTATAC+CGG 0.512209 3:+19588856 MsaT012133.1:CDS
TATCTTCCCCGCGGTCACCC+TGG 0.516259 3:-19588780 None:intergenic
ATTCTTGCTGACCTCTCCTC+GGG 0.517115 3:+19589201 MsaT012133.1:CDS
TCAATGGAGTTCTGGCGAAT+CGG 0.519477 3:+19589436 MsaT012133.1:CDS
GTATAAAATTCAAGATTCAT+GGG 0.521482 3:+19589047 MsaT012133.1:CDS
TATAAGTTGATAAGAGTGCC+TGG 0.523531 3:+19589105 MsaT012133.1:CDS
TTTGCGTGCACTGCTACCCT+CGG 0.525087 3:-19589994 None:intergenic
TCAACACTCAGTTCCTCCCC+CGG 0.526455 3:+19588108 MsaT012133.1:CDS
TGACCGCGGGGAAGATACTT+TGG 0.527412 3:+19588786 MsaT012133.1:CDS
GGTTTAGTTGGTATAAGCCC+AGG 0.529198 3:+19588463 MsaT012133.1:CDS
AAACGTACCGATTTAAAAGC+TGG 0.530169 3:+19588601 MsaT012133.1:CDS
GTTGAAATTCCCACCCTGAC+CGG 0.530593 3:-19588875 None:intergenic
TCATATGTTAACTCATGCAT+TGG 0.532861 3:-19589954 None:intergenic
CGGCAGCATCACATTCAAGC+CGG 0.533696 3:+19588389 MsaT012133.1:CDS
TCAAAACAAAGGATAAAGGT+TGG 0.534159 3:+19589556 MsaT012133.1:CDS
GGTAGAAAGAAATGCTTATG+TGG 0.534383 3:+19590017 MsaT012133.1:CDS
TCATGGGATCCTATTCGTGC+TGG 0.535655 3:+19589588 MsaT012133.1:CDS
TCAGATCAGAAGCTTCATAA+GGG 0.535931 3:+19588916 MsaT012133.1:CDS
ACTGCTACCCTCGGTGTGAT+CGG 0.536665 3:-19589985 None:intergenic
TGAATTGTATAATGGAAGAA+GGG 0.540125 3:+19588080 None:intergenic
GGAGTCGGCATCGCTCTACT+TGG 0.540336 3:-19588289 None:intergenic
AGGTTGGGGTCTTCGATCAT+GGG 0.540693 3:+19589572 MsaT012133.1:CDS
AAAACAAAGGATAAAGGTTG+GGG 0.541211 3:+19589558 MsaT012133.1:CDS
GGCAATATTTGTATGACCAA+AGG 0.542268 3:+19588622 MsaT012133.1:CDS
GGAAAGCTGGTTTCCCTGCA+AGG 0.545796 3:+19589169 MsaT012133.1:CDS
GACTCTCGGCTCCCGAGGAG+AGG 0.546001 3:-19589212 None:intergenic
ATCTCAACTTGTAGATGCCA+AGG 0.547043 3:+19588558 MsaT012133.1:CDS
ATGCAACATGGAGAAAGGAT+TGG 0.548940 3:-19589908 None:intergenic
TTCCAAACGGCAAAGGCACT+AGG 0.550397 3:-19589132 None:intergenic
GATCCTCATCCTCTCCAAAA+GGG 0.552939 3:-19588330 None:intergenic
AACTTGTAGATGCCAAGGAA+TGG 0.553451 3:+19588563 MsaT012133.1:CDS
TTTGGAAATCTGTTCAGAAG+TGG 0.553589 3:+19589148 MsaT012133.1:CDS
ATGTCCCCAATCTCAACTCC+AGG 0.554821 3:-19588682 None:intergenic
TCTTAGCATTTATAATAAGA+GGG 0.554977 3:-19589803 None:intergenic
GGCAGCATCACATTCAAGCC+GGG 0.555680 3:+19588390 MsaT012133.1:CDS
GTATTTATGAATCTTTCAAG+TGG 0.556979 3:+19589712 MsaT012133.1:CDS
GCAAAGGCACTAGGTTGTCC+AGG 0.558915 3:-19589123 None:intergenic
TTATACTTGTCAATGGAACC+CGG 0.562427 3:-19588127 None:intergenic
GGTCAGATTGAAGAAGCATA+AGG 0.562915 3:+19588411 MsaT012133.1:CDS
TCACATAGTTGCAGCTGTAG+TGG 0.565201 3:+19589339 MsaT012133.1:CDS
GCATTTATAATAAGAGGGTG+TGG 0.566087 3:-19589798 None:intergenic
CTAAATGCTTGAAGTGGAGT+CGG 0.566439 3:-19588304 None:intergenic
GAGAAGTTATCGATAAAGCT+AGG 0.566713 3:+19589634 MsaT012133.1:CDS
GTTATAATTTATACCGGTCA+GGG 0.567735 3:+19588862 MsaT012133.1:CDS
ATTTCGGATGTCTAAGAGAG+TGG 0.569188 3:-19589296 None:intergenic
AAGCCGTTGGTTCATGAATG+TGG 0.569854 3:+19589459 MsaT012133.1:CDS
GAGGATCTCAATGACAATGA+TGG 0.572099 3:+19588346 MsaT012133.1:CDS
TATGCTTCTTCAATCTGACC+CGG 0.573333 3:-19588408 None:intergenic
TCGAACAAACACAGCGAAGA+GGG 0.575127 3:+19588648 MsaT012133.1:CDS
TTGCATATCCAGGGTGACCG+CGG 0.575259 3:+19588772 MsaT012133.1:CDS
TATGTTAACTCATGCATTGG+TGG 0.577942 3:-19589951 None:intergenic
GCCTAAGTTACTAGAAGAAG+CGG 0.581948 3:+19589743 MsaT012133.1:CDS
TGCATGCTCAATCAATGGGG+GGG 0.583193 3:+19588740 MsaT012133.1:CDS
AACAGATTTCCAAACGGCAA+AGG 0.589578 3:-19589139 None:intergenic
GGGCTCCTAAATGCTTGAAG+TGG 0.589829 3:-19588310 None:intergenic
TTAAGTCAGCTTGTACAAGA+AGG 0.590145 3:+19589657 MsaT012133.1:CDS
AATGGAAGTGTTCAAAACAA+AGG 0.598701 3:+19589545 MsaT012133.1:CDS
AAGTGATACTGGAAGACTCT+CGG 0.599204 3:-19589226 None:intergenic
CTTCTGAACAGATTTCCAAA+CGG 0.599772 3:-19589145 None:intergenic
TATACTTGTCAATGGAACCC+GGG 0.600215 3:-19588126 None:intergenic
TGACCTGAAGATTGACCTTG+AGG 0.600787 3:-19588202 None:intergenic
GAATAAGTCCAGTCCTTGCA+GGG 0.601962 3:-19589182 None:intergenic
CATTTGATGCGCTAAGACGA+AGG 0.602005 3:+19588533 MsaT012133.1:CDS
AAGAGGGTAGGATCAGTACC+TGG 0.602661 3:+19588664 MsaT012133.1:CDS
GGTCACAAATGAGTCTAATG+AGG 0.609516 3:+19589764 MsaT012133.1:CDS
GGGCCACATTCATGAACCAA+CGG 0.610204 3:-19589462 None:intergenic
TCTGGCGAATCGGAAGCCGT+TGG 0.612003 3:+19589446 MsaT012133.1:CDS
TGCTAAGAATGTTGGAAATG+TGG 0.613562 3:+19589818 MsaT012133.1:CDS
GATGTTCATGTTGATATGAG+TGG 0.616806 3:+19588442 MsaT012133.1:CDS
TGGAAGACTCTCGGCTCCCG+AGG 0.617476 3:-19589217 None:intergenic
AGCCCAGGTCAAAGAGACAA+TGG 0.619161 3:+19588478 MsaT012133.1:CDS
TAATTTATACCGGTCAGGGT+GGG 0.620279 3:+19588866 MsaT012133.1:CDS
TTAGGAAAACACTGACATGT+GGG 0.620821 3:-19589483 None:intergenic
GTTTGCATGCTCAATCAATG+GGG 0.626097 3:+19588737 MsaT012133.1:CDS
TACGACGATGAAGCTGAGGA+TGG 0.627250 3:+19588835 MsaT012133.1:CDS
ACCGCTTCTTCTAGTAACTT+AGG 0.628944 3:-19589744 None:intergenic
GCGGTCACCCTGGATATGCA+AGG 0.632039 3:-19588770 None:intergenic
ACAAACACAGCGAAGAGGGT+AGG 0.634440 3:+19588652 MsaT012133.1:CDS
CTACAGCTGCAACTATGTGA+AGG 0.634477 3:-19589336 None:intergenic
ATACTTGTCAATGGAACCCG+GGG 0.636158 3:-19588125 None:intergenic
ATTGATGCCTTGCATATCCA+GGG 0.637842 3:+19588763 MsaT012133.1:CDS
GTTAGGAAAACACTGACATG+TGG 0.639912 3:-19589484 None:intergenic
GATGCCAAGGAATGGAACAC+AGG 0.640965 3:+19588571 MsaT012133.1:CDS
AGAATACGACGATGAAGCTG+AGG 0.645222 3:+19588831 MsaT012133.1:CDS
TTCGAACAAACACAGCGAAG+AGG 0.646517 3:+19588647 MsaT012133.1:CDS
TTGCATGCTCAATCAATGGG+GGG 0.648036 3:+19588739 MsaT012133.1:CDS
GAGGGTAGCAGTGCACGCAA+AGG 0.654567 3:+19589996 MsaT012133.1:CDS
TATGGTTCCGATCACACCGA+GGG 0.656242 3:+19589978 MsaT012133.1:CDS
AGTACCTGGAGTTGAGATTG+GGG 0.657916 3:+19588678 MsaT012133.1:CDS
TCATGCATTCGGAGAAACGA+AGG 0.659918 3:+19589396 MsaT012133.1:CDS
TTTGCAGTTGAAGTATCATG+AGG 0.660591 3:-19588265 None:intergenic
ATAATTTATACCGGTCAGGG+TGG 0.675305 3:+19588865 MsaT012133.1:CDS
TTTGCATGCTCAATCAATGG+GGG 0.680195 3:+19588738 MsaT012133.1:CDS
TGTGGATCATCAAACTGCCG+CGG 0.682422 3:+19590035 MsaT012133.1:CDS
ATGAGCAGTCCAAATCGCCA+GGG 0.685642 3:-19589377 None:intergenic
TAGGAAAACACTGACATGTG+GGG 0.688446 3:-19589482 None:intergenic
GCATATCCAGGGTGACCGCG+GGG 0.702296 3:+19588774 MsaT012133.1:CDS
AATGTCAAGTAAAAGAACCG+CGG 0.738828 3:-19590052 None:intergenic
AATGAAATAAGAGTCATCCG+AGG 0.740934 3:+19588973 MsaT012133.1:CDS
CAGCCAAAGTATCTTCCCCG+CGG 0.752822 3:-19588789 None:intergenic
TTATGGTTCCGATCACACCG+AGG 0.773964 3:+19589977 MsaT012133.1:CDS
TACTTGTCAATGGAACCCGG+GGG 0.840303 3:-19588124 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 19588091 19590070 19588091 ID=MsaG012133
Chr3 mRNA 19588091 19590070 19588091 ID=MsaT012133.1;Parent=MsaG012133
Chr3 exon 19588091 19590070 19588091 ID=MsaT012133.1.exon1;Parent=MsaT012133.1
Chr3 CDS 19588091 19590070 19588091 ID=cds.MsaT012133.1;Parent=MsaT012133.1
Gene Sequence

>MsaT012133.1

ATGGAAGAAGGGTTAGGTCAACACTCAGTTCCTCCCCCGGGTTCCATTGACAAGTATAAGATTCTGGATATTAAGCCTATAAGAAGTTTGATACCTGTTTTTTCAAGGAATCCTCAAGGTCAATCTTCAGGTCAGTATCCTTCTGGGTTTTCTCCATTTTTCCCATTTGGTGGACCTCATGATACTTCAACTGCAAAGCCAAGTAGAGCGATGCCGACTCCACTTCAAGCATTTAGGAGCCCTTTTGGAGAGGATGAGGATCTCAATGACAATGATGGTTTTAGCAACAAAAGAAGTGCGGCAGCATCACATTCAAGCCGGGTCAGATTGAAGAAGCATAAGGTATATAATGATGTTCATGTTGATATGAGTGGTTTAGTTGGTATAAGCCCAGGTCAAAGAGACAATGGTAACCGTGAAGTTGTTAATATTGTGCTCATGACATTTGATGCGCTAAGACGAAGGCTATCTCAACTTGTAGATGCCAAGGAATGGAACACAGGTTCGATCAAACGTACCGATTTAAAAGCTGGCAATATTTGTATGACCAAAGGAATTCGAACAAACACAGCGAAGAGGGTAGGATCAGTACCTGGAGTTGAGATTGGGGACATTTTCTTTTTCCGAATGGAAATGTGTGTTATCGGTTTGCATGCTCAATCAATGGGGGGGATTGATGCCTTGCATATCCAGGGTGACCGCGGGGAAGATACTTTGGCTGTTAGCATTGTTTCGTCAGGAGAATACGACGATGAAGCTGAGGATGGTGATGTTATAATTTATACCGGTCAGGGTGGGAATTTCAACAAGAAAGATAAGCATGTGTCAGATCAGAAGCTTCATAAGGGTAATCTTGCTTTGGATAGAAGTTCACGAACACACAATGAAATAAGAGTCATCCGAGGCATCAAAGATGCTGTGACTCCAGGTGCAAAAATCTATGTCTATGATGGTCTGTATAAAATTCAAGATTCATGGGTTGAAAAAGCAAAAGGAGGCGGTGGCTTGTTTAAGTATAAGTTGATAAGAGTGCCTGGACAACCTAGTGCCTTTGCCGTTTGGAAATCTGTTCAGAAGTGGAAAGCTGGTTTCCCTGCAAGGACTGGACTTATTCTTGCTGACCTCTCCTCGGGAGCCGAGAGTCTTCCAGTATCACTTGTCAATGAAGTCGATAACGTTAAGTCACCTGCCTTTTTCACATATTTCCACTCTCTTAGACATCCGAAATCATTCAGTTTAATGCAGCCTTCACATAGTTGCAGCTGTAGTGGTAAAAAAGCATGTGTCCCTGGCGATTTGGACTGCTCATGCATTCGGAGAAACGAAGGTGATTTTCCTTATATTATCAATGGAGTTCTGGCGAATCGGAAGCCGTTGGTTCATGAATGTGGCCCCACATGTCAGTGTTTTCCTAACTGCAAAAATCGAGTATCCCAAACCGGTTTGAAGCACCAAATGGAAGTGTTCAAAACAAAGGATAAAGGTTGGGGTCTTCGATCATGGGATCCTATTCGTGCTGGTGCTTTTATTTGCGAGTATGCAGGAGAAGTTATCGATAAAGCTAGGTTAAGTCAGCTTGTACAAGAAGGAGATACTGATGAGTATGTTTTCGATACAACCCGTATTTATGAATCTTTCAAGTGGAATTATGAGCCTAAGTTACTAGAAGAAGCGGTCACAAATGAGTCTAATGAGGATTATGCTTTGCCACACCCTCTTATTATAAATGCTAAGAATGTTGGAAATGTGGCTAGATTCATGAATCATAGTTGCTCTCCAAATGTTTTCTGGCAGCCTGTCTTATACGAAGAGAACAACCAATCCTTTCTCCATGTTGCATTTTTTGCATTGAGACATATTCCACCAATGCATGAGTTAACATATGATTATGGTTCCGATCACACCGAGGGTAGCAGTGCACGCAAAGGTAGAAAGAAATGCTTATGTGGATCATCAAACTGCCGCGGTTCTTTTACTTGA
Protein sequence

>MsaT012133.1

MEEGLGQHSVPPPGSIDKYKILDIKPIRSLIPVFSRNPQGQSSGQYPSGFSPFFPFGGPHDTSTAKPSRAMPTPLQAFRSPFGEDEDLNDNDGFSNKRSAAASHSSRVRLKKHKVYNDVHVDMSGLVGISPGQRDNGNREVVNIVLMTFDALRRRLSQLVDAKEWNTGSIKRTDLKAGNICMTKGIRTNTAKRVGSVPGVEIGDIFFFRMEMCVIGLHAQSMGGIDALHIQGDRGEDTLAVSIVSSGEYDDEAEDGDVIIYTGQGGNFNKKDKHVSDQKLHKGNLALDRSSRTHNEIRVIRGIKDAVTPGAKIYVYDGLYKIQDSWVEKAKGGGGLFKYKLIRVPGQPSAFAVWKSVQKWKAGFPARTGLILADLSSGAESLPVSLVNEVDNVKSPAFFTYFHSLRHPKSFSLMQPSHSCSCSGKKACVPGDLDCSCIRRNEGDFPYIINGVLANRKPLVHECGPTCQCFPNCKNRVSQTGLKHQMEVFKTKDKGWGLRSWDPIRAGAFICEYAGEVIDKARLSQLVQEGDTDEYVFDTTRIYESFKWNYEPKLLEEAVTNESNEDYALPHPLIINAKNVGNVARFMNHSCSPNVFWQPVLYEENNQSFLHVAFFALRHIPPMHELTYDYGSDHTEGSSARKGRKKCLCGSSNCRGSFT*