Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012142 | ABN08117.1 | 77.311 | 119 | 27 | 0 | 58 | 176 | 1 | 119 | 6.38e-58 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012142 | A2Q3B7 | 77.311 | 119 | 27 | 0 | 58 | 176 | 1 | 119 | 3.05e-58 | 187 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG012142 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012142 | MtrunA17_Chr2g0309121 | 77.311 | 119 | 27 | 0 | 58 | 176 | 1 | 119 | 5.87e-62 | 187 |
| MsaG012142 | MtrunA17_Chr2g0309121 | 80.000 | 40 | 8 | 0 | 1 | 40 | 1 | 40 | 7.71e-14 | 64.7 |
| MsaG012142 | MtrunA17_Chr2g0281511 | 77.500 | 120 | 26 | 1 | 58 | 176 | 1 | 120 | 2.94e-61 | 186 |
| MsaG012142 | MtrunA17_Chr3g0081891 | 71.250 | 80 | 22 | 1 | 58 | 136 | 1 | 80 | 3.89e-33 | 113 |
| MsaG012142 | MtrunA17_Chr3g0081891 | 51.250 | 80 | 31 | 2 | 1 | 79 | 1 | 73 | 4.61e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTCAACGGTCCTGAATT+AGG | 0.221297 | 3:-19680361 | MsaT012142.1:CDS |
| GAAGTCAACGGTCCTGAATT+AGG | 0.221297 | 3:-19680786 | MsaT012142.1:CDS |
| AAAATGCCGATGTTCATTAT+TGG | 0.252563 | 3:-19680330 | MsaT012142.1:CDS |
| AAAATGTCGATGTTCATTAT+TGG | 0.262235 | 3:-19680755 | MsaT012142.1:CDS |
| TAATGTTATTGCTTCTACTT+TGG | 0.311699 | 3:-19680302 | MsaT012142.1:CDS |
| TAATGTTATTGCTTCTACTT+TGG | 0.311699 | 3:-19680727 | MsaT012142.1:CDS |
| GTGAAGAAAAGTAAATCTTA+TGG | 0.319603 | 3:-19680102 | MsaT012142.1:CDS |
| GTAGCATACATCCTATTAAA+AGG | 0.335252 | 3:+19679958 | None:intergenic |
| TGTTTATGTTATATATCATT+TGG | 0.337645 | 3:-19680414 | MsaT012142.1:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.360997 | 3:-19680284 | MsaT012142.1:intron |
| TTTGGATGGAAACAATGTTT+TGG | 0.360997 | 3:-19680709 | MsaT012142.1:intron |
| GTACGGCTTTGTTCTTGAAA+AGG | 0.418940 | 3:-19680022 | MsaT012142.1:CDS |
| AAGTCAACGGTCCTGAATTA+GGG | 0.429565 | 3:-19680360 | MsaT012142.1:CDS |
| AAGTCAACGGTCCTGAATTA+GGG | 0.429565 | 3:-19680785 | MsaT012142.1:CDS |
| GTTATTGCTTCTACTTTGGA+TGG | 0.442931 | 3:-19680298 | MsaT012142.1:CDS |
| GTTATTGCTTCTACTTTGGA+TGG | 0.442931 | 3:-19680723 | MsaT012142.1:CDS |
| TACGGCTTTGTTCTTGAAAA+GGG | 0.445793 | 3:-19680021 | MsaT012142.1:CDS |
| GCATGCATCAGAAGTACATA+GGG | 0.496344 | 3:-19680053 | MsaT012142.1:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 3:-19680009 | MsaT012142.1:CDS |
| AGCATGCATCAGAAGTACAT+AGG | 0.546367 | 3:-19680054 | MsaT012142.1:CDS |
| CATCAGAAGTACATAGGGAG+AGG | 0.578101 | 3:-19680048 | MsaT012142.1:CDS |
| AGAAGTACATAGGGAGAGGA+TGG | 0.580923 | 3:-19680044 | MsaT012142.1:CDS |
| TACATAGGGAGAGGATGGTA+CGG | 0.597869 | 3:-19680039 | MsaT012142.1:CDS |
| ATACTTCCAATAATGAACAT+CGG | 0.656056 | 3:+19680324 | None:intergenic |
| ACATTCTCAGCAACACCTCG+AGG | 0.684927 | 3:+19680159 | None:intergenic |
| GAGACTGGAGTTGAAGTCAA+CGG | 0.686388 | 3:-19680373 | MsaT012142.1:CDS |
| GAGACTGGAGTTGAAGTCAA+CGG | 0.686388 | 3:-19680798 | MsaT012142.1:CDS |
| GAATTAGTCTCAGTTAACGA+CGG | 0.726011 | 3:+19679936 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 19679945 | 19680826 | 19679945 | ID=MsaG012142 |
| Chr3 | mRNA | 19679945 | 19680826 | 19679945 | ID=MsaT012142.1;Parent=MsaG012142 |
| Chr3 | exon | 19679945 | 19680187 | 19679945 | ID=MsaT012142.1.exon3;Parent=MsaT012142.1 |
| Chr3 | CDS | 19679945 | 19680187 | 19679945 | ID=cds.MsaT012142.1;Parent=MsaT012142.1 |
| Chr3 | exon | 19680285 | 19680455 | 19680285 | ID=MsaT012142.1.exon2;Parent=MsaT012142.1 |
| Chr3 | CDS | 19680285 | 19680455 | 19680285 | ID=cds.MsaT012142.1;Parent=MsaT012142.1 |
| Chr3 | exon | 19680710 | 19680826 | 19680710 | ID=MsaT012142.1.exon1;Parent=MsaT012142.1 |
| Chr3 | CDS | 19680710 | 19680826 | 19680710 | ID=cds.MsaT012142.1;Parent=MsaT012142.1 |
| Gene Sequence |
| Protein sequence |
