AlfalfaGEDB | MsaG012176

M. sativa ssp. caerulea / MsaG012176

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007602 GO:0007623 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009639 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010017 GO:0010018 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042752 GO:0042753 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0080090 GO:0104004 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012176	TXG57744.1	65.635	646	163	6	1	616	1	617	0.0	865

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012176	sp\|Q9LIE5\|FHY3_ARATH	54.315	394	152	4	250	617	448	839	3.93e-132	410
MsaG012176	sp\|Q9LIE5\|FHY3_ARATH	63.636	253	85	3	1	252	1	247	4.49e-100	326

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012176	A0A5C7HLI5	65.635	646	163	6	1	616	1	617	0.0	865

TFs/TRs:

Gene ID	Type	Classification
MsaG012176	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002811	MsaG012176	0.803478	5.757562e-49	8.426188e-47
MsaG003638	MsaG012176	0.805192	2.540752e-49	3.869255e-47
MsaG005730	MsaG012176	0.807455	8.518880e-50	1.368293e-47
MsaG006260	MsaG012176	0.814338	2.800134e-51	5.323445e-49
MsaG007443	MsaG012176	0.811633	1.089742e-50	1.936725e-48
MsaG009738	MsaG012176	0.825966	6.248713e-54	1.614495e-51
MsaG009860	MsaG012176	0.847987	1.544361e-59	7.817873e-57
MsaG011397	MsaG012176	0.803335	6.162193e-49	8.988574e-47
MsaG011911	MsaG012176	0.841162	1.041073e-57	4.215555e-55
MsaG012176	MsaG017630	0.811969	9.214344e-51	1.651232e-48
MsaG012176	MsaG023909	0.840604	1.455686e-57	5.791483e-55
MsaG012176	MsaG028705	0.816074	1.156714e-51	2.297719e-49
MsaG012176	MsaG028853	0.815249	1.763057e-51	3.429733e-49
MsaG012176	MsaG031327	0.801236	1.658924e-48	2.306371e-46
MsaG012176	MsaG044632	0.825722	7.133653e-54	1.830666e-51
MsaG012176	MsaG045859	0.809022	3.961374e-50	6.607261e-48
MsaG012176	MsaG046025	0.807850	7.027964e-50	1.139601e-47
MsaG012176	MsaG031244	0.802113	1.098323e-48	1.557724e-46
MsaG012176	MsaG043209	0.811817	9.941902e-51	1.774954e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012176	MtrunA17_Chr3g0080351	90.500	400	11	2	242	617	448	844	0.0	714
MsaG012176	MtrunA17_Chr3g0080351	96.825	252	8	0	1	252	1	252	1.47e-165	494
MsaG012176	MtrunA17_Chr1g0207551	47.146	403	179	6	243	617	415	811	2.57e-106	339
MsaG012176	MtrunA17_Chr1g0207551	43.363	226	90	3	49	251	7	217	1.97e-44	169
MsaG012176	MtrunA17_Chr2g0329031	42.434	304	158	4	252	540	394	695	1.32e-67	234
MsaG012176	MtrunA17_Chr2g0329031	43.689	206	83	4	63	251	1	190	3.05e-38	150
MsaG012176	MtrunA17_Chr5g0448761	34.818	247	154	3	254	493	458	704	7.68e-40	155
MsaG012176	MtrunA17_Chr5g0448761	37.778	135	63	3	68	196	52	171	1.18e-15	80.9
MsaG012176	MtrunA17_Chr5g0440451	34.906	212	134	2	265	472	448	659	1.85e-34	140
MsaG012176	MtrunA17_Chr3g0118611	26.946	334	236	4	265	591	345	677	1.17e-33	137
MsaG012176	MtrunA17_Chr5g0448511	33.214	280	174	6	260	530	236	511	3.01e-32	131
MsaG012176	MtrunA17_Chr2g0291581	33.766	231	141	4	252	470	445	675	2.06e-31	130
MsaG012176	MtrunA17_Chr6g0477261	39.326	178	101	2	252	422	270	447	3.95e-30	125
MsaG012176	MtrunA17_Chr2g0296781	39.326	178	101	2	252	422	381	558	1.74e-29	124
MsaG012176	MtrunA17_Chr1g0202341	33.921	227	141	4	252	470	440	665	6.41e-29	122
MsaG012176	MtrunA17_Chr5g0441581	32.877	219	138	2	250	459	407	625	1.23e-28	121
MsaG012176	MtrunA17_Chr1g0196541	36.683	199	116	3	252	443	381	576	9.56e-28	119
MsaG012176	MtrunA17_Chr3g0082121	27.632	228	153	4	252	472	425	647	9.04e-18	87.4
MsaG012176	MtrunA17_Chr1g0198761	30.348	201	124	6	236	433	434	621	4.12e-16	82.0
MsaG012176	MtrunA17_Chr1g0203721	39.286	112	63	1	364	470	567	678	7.53e-16	81.6
MsaG012176	MtrunA17_Chr2g0329361	34.146	123	70	2	74	196	59	170	1.36e-15	77.0
MsaG012176	MtrunA17_Chr3g0078041	28.037	214	113	5	75	287	49	222	1.66e-15	76.3
MsaG012176	MtrunA17_Chr2g0324011	26.923	182	129	2	253	430	400	581	2.15e-15	79.7
MsaG012176	MtrunA17_Chr2g0287061	30.000	220	142	4	261	472	540	755	7.43e-13	71.6
MsaG012176	MtrunA17_Chr2g0294841	27.711	166	113	3	252	415	443	603	1.68e-12	70.5
MsaG012176	MtrunA17_Chr7g0233911	33.871	124	54	3	75	190	33	136	2.27e-12	66.6
MsaG012176	MtrunA17_Chr4g0033611	29.444	180	94	6	54	233	55	201	3.12e-12	67.4
MsaG012176	MtrunA17_Chr2g0276841	29.167	168	111	5	252	417	465	626	3.54e-12	69.7
MsaG012176	MtrunA17_Chr6g0458391	29.448	163	111	2	261	421	606	766	7.44e-12	68.6
MsaG012176	MtrunA17_Chr2g0287651	27.083	240	164	7	254	485	464	700	1.05e-11	68.2
MsaG012176	MtrunA17_Chr2g0329421	30.579	121	70	2	75	195	26	132	1.18e-11	64.7
MsaG012176	MtrunA17_Chr8g0363841	26.554	177	117	4	252	420	406	577	3.51e-11	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012176	AT3G22170.2	54.315	394	152	4	250	617	448	839	3.99e-133	410
MsaG012176	AT3G22170.2	63.636	253	85	3	1	252	1	247	4.57e-101	326
MsaG012176	AT3G22170.1	54.315	394	152	4	250	617	448	839	3.99e-133	410
MsaG012176	AT3G22170.1	63.636	253	85	3	1	252	1	247	4.57e-101	326
MsaG012176	AT4G15090.1	44.724	398	190	7	242	615	434	825	2.48e-105	337
MsaG012176	AT4G15090.1	41.176	255	94	7	27	247	1	233	4.68e-44	169
MsaG012176	AT4G15090.5	44.724	398	190	7	242	615	434	825	2.48e-105	337
MsaG012176	AT4G15090.5	41.176	255	94	7	27	247	1	233	4.68e-44	169
MsaG012176	AT4G15090.3	44.724	398	190	7	242	615	434	825	2.48e-105	337
MsaG012176	AT4G15090.3	41.176	255	94	7	27	247	1	233	4.68e-44	169
MsaG012176	AT4G15090.2	44.724	398	190	7	242	615	434	825	2.48e-105	337
MsaG012176	AT4G15090.2	41.176	255	94	7	27	247	1	233	4.68e-44	169
MsaG012176	AT4G15090.4	44.724	398	190	7	242	615	434	825	2.48e-105	337
MsaG012176	AT4G15090.4	41.176	255	94	7	27	247	1	233	4.68e-44	169
MsaG012176	AT1G76320.3	44.444	252	138	1	252	503	378	627	1.12e-67	233
MsaG012176	AT1G76320.3	40.000	190	80	3	80	251	1	174	1.69e-32	133
MsaG012176	AT1G76320.1	36.339	366	213	7	252	612	378	728	1.69e-67	234
MsaG012176	AT1G76320.1	40.000	190	80	3	80	251	1	174	1.71e-32	134
MsaG012176	AT1G76320.4	36.339	366	213	7	252	612	378	728	1.69e-67	234
MsaG012176	AT1G76320.4	40.000	190	80	3	80	251	1	174	1.71e-32	134
MsaG012176	AT1G76320.2	44.444	252	138	1	252	503	378	627	1.91e-67	234
MsaG012176	AT1G76320.2	40.000	190	80	3	80	251	1	174	1.88e-32	133
MsaG012176	AT4G19990.1	43.155	336	181	4	252	584	332	660	5.98e-63	221
MsaG012176	AT4G19990.1	41.667	144	56	6	116	251	4	127	2.43e-18	89.7
MsaG012176	AT4G19990.5	43.155	336	181	4	252	584	389	717	8.65e-63	221
MsaG012176	AT4G19990.5	44.920	187	75	6	73	251	18	184	1.18e-32	134
MsaG012176	AT4G19990.3	43.155	336	181	4	252	584	389	717	8.95e-63	221
MsaG012176	AT4G19990.3	44.920	187	75	6	73	251	18	184	1.11e-32	134
MsaG012176	AT4G19990.4	43.155	336	181	4	252	584	389	717	8.95e-63	221
MsaG012176	AT4G19990.4	44.920	187	75	6	73	251	18	184	1.11e-32	134
MsaG012176	AT4G19990.7	43.155	336	181	4	252	584	403	731	9.63e-63	221
MsaG012176	AT4G19990.7	41.627	209	91	7	51	251	13	198	9.64e-33	134
MsaG012176	AT4G19990.6	43.155	336	181	4	252	584	389	717	1.30e-62	221
MsaG012176	AT4G19990.6	44.920	187	75	6	73	251	18	184	1.33e-32	134
MsaG012176	AT4G19990.2	43.155	336	181	4	252	584	389	717	1.30e-62	221
MsaG012176	AT4G19990.2	44.920	187	75	6	73	251	18	184	1.33e-32	134
MsaG012176	AT2G32250.5	33.607	366	204	6	252	587	385	741	3.41e-51	189
MsaG012176	AT2G32250.5	51.304	115	40	1	78	192	40	138	4.42e-27	117
MsaG012176	AT2G32250.7	33.607	366	204	6	252	587	344	700	3.61e-51	189
MsaG012176	AT2G32250.7	51.327	113	39	1	80	192	1	97	3.52e-26	114
MsaG012176	AT2G32250.8	33.607	366	204	6	252	587	344	700	3.79e-51	189
MsaG012176	AT2G32250.8	51.327	113	39	1	80	192	1	97	3.41e-26	114
MsaG012176	AT2G32250.2	33.607	366	204	6	252	587	385	741	4.07e-51	189
MsaG012176	AT2G32250.2	51.304	115	40	1	78	192	40	138	4.70e-27	117
MsaG012176	AT2G32250.4	33.607	366	204	6	252	587	385	741	4.07e-51	189
MsaG012176	AT2G32250.4	51.304	115	40	1	78	192	40	138	4.70e-27	117
MsaG012176	AT2G32250.6	33.607	366	204	6	252	587	385	741	4.92e-51	189
MsaG012176	AT2G32250.6	51.304	115	40	1	78	192	40	138	5.11e-27	117
MsaG012176	AT2G32250.1	33.607	366	204	6	252	587	385	741	4.92e-51	189
MsaG012176	AT2G32250.1	51.304	115	40	1	78	192	40	138	5.11e-27	117
MsaG012176	AT2G32250.3	33.607	366	204	6	252	587	385	741	4.92e-51	189
MsaG012176	AT2G32250.3	51.304	115	40	1	78	192	40	138	5.11e-27	117
MsaG012176	AT4G38180.1	30.921	304	193	5	252	540	465	766	3.58e-35	142
MsaG012176	AT4G38180.1	30.622	209	100	5	73	252	70	262	1.17e-17	87.4
MsaG012176	AT4G38170.1	34.914	232	143	3	254	479	232	461	7.03e-35	139
MsaG012176	AT2G27110.3	35.616	219	131	4	265	477	278	492	8.56e-34	137
MsaG012176	AT2G27110.2	35.047	214	130	3	265	472	423	633	1.21e-33	137
MsaG012176	AT2G27110.1	35.047	214	130	3	265	472	423	633	1.21e-33	137
MsaG012176	AT1G52520.1	35.622	233	134	6	252	470	461	691	3.96e-31	129
MsaG012176	AT1G80010.1	29.245	212	142	3	267	470	502	713	5.10e-22	101
MsaG012176	AT1G10240.2	26.778	239	162	6	252	483	443	675	4.82e-17	85.5
MsaG012176	AT1G10240.1	26.778	239	162	6	252	483	443	675	4.82e-17	85.5
MsaG012176	AT2G43280.1	29.050	179	110	3	57	230	3	169	7.78e-16	77.0
MsaG012176	AT3G59470.3	30.935	139	82	2	57	195	51	175	2.41e-15	76.6
MsaG012176	AT3G59470.1	30.935	139	82	2	57	195	51	175	2.41e-15	76.6
MsaG012176	AT3G59470.2	30.935	139	82	2	57	195	63	187	3.09e-15	76.6
MsaG012176	AT3G06250.2	28.571	217	146	4	262	472	545	758	8.86e-14	75.1
MsaG012176	AT3G06250.2	31.667	120	56	2	75	194	189	282	4.64e-11	66.6
MsaG012176	AT3G06250.1	28.571	217	146	4	262	472	545	758	8.86e-14	75.1
MsaG012176	AT3G06250.1	31.667	120	56	2	75	194	189	282	4.64e-11	66.6
MsaG012176	AT3G06250.3	28.571	217	146	4	262	472	545	758	8.86e-14	75.1
MsaG012176	AT3G06250.3	31.667	120	56	2	75	194	189	282	4.64e-11	66.6
MsaG012176	AT3G07500.1	33.835	133	72	2	74	206	31	147	8.97e-14	71.2
MsaG012176	AT5G18960.2	28.704	216	147	4	262	472	569	782	1.73e-13	74.3
MsaG012176	AT5G18960.2	33.333	126	58	3	69	194	204	303	2.43e-11	67.4
MsaG012176	AT5G18960.1	28.704	216	147	4	262	472	569	782	1.73e-13	74.3
MsaG012176	AT5G18960.1	33.333	126	58	3	69	194	204	303	2.43e-11	67.4
MsaG012176	AT4G12850.4	32.759	116	62	2	75	190	10	109	4.29e-12	64.7
MsaG012176	AT4G12850.2	32.800	125	67	3	67	190	1	109	6.23e-12	63.9
MsaG012176	AT4G12850.1	32.759	116	62	2	75	190	10	109	6.84e-12	65.1

Find 138 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTCGATAAAGTTCGTAATT+TGG	0.150148	3:+20255183	MsaT012176.1:CDS
AGTTGATATTGCAATGTTTA+AGG	0.172219	3:+20254691	MsaT012176.1:CDS
ACAAATCTTGAGCCACTTTC+TGG	0.189659	3:+20254719	MsaT012176.1:CDS
CGTAATTTGGGTCTGGAGTT+TGG	0.217600	3:+20255196	MsaT012176.1:CDS
GATTGTTCAGTTGAACTTAA+TGG	0.243913	3:+20257212	MsaT012176.1:intron
TTGGTGGTTCTTCAATATTC+TGG	0.244129	3:+20256297	MsaT012176.1:CDS
CTGGTTTCGCATACAGCATT+AGG	0.246092	3:+20257531	MsaT012176.1:CDS
AGATACCCAAAGTAGAAATA+TGG	0.254157	3:+20256614	MsaT012176.1:CDS
GTAATTTGGGTCTGGAGTTT+GGG	0.256919	3:+20255197	MsaT012176.1:CDS
TTCGATAAAGTTCGTAATTT+GGG	0.268268	3:+20255184	MsaT012176.1:CDS
CTTTCCCATATTTCTACTTT+GGG	0.301786	3:-20256619	None:intergenic
CCTTTAGAAAAGAGTGTTGC+AGG	0.305818	3:+20256054	MsaT012176.1:CDS
ATTGTTCAGTTGAACTTAAT+GGG	0.306375	3:+20257213	MsaT012176.1:intron
AAAGTTTGCTTGTTCGGATA+TGG	0.307047	3:+20254865	MsaT012176.1:CDS
CCATTGATCGAGCATATTCT+TGG	0.307582	3:-20254781	None:intergenic
ACCAGCCACGATGGTTATTA+TGG	0.311286	3:+20257383	MsaT012176.1:CDS
AAGATCCAAGCTGAGATTAT+TGG	0.311613	3:+20256102	MsaT012176.1:CDS
TTGTTTGTTCACTTACTGCC+TGG	0.318808	3:-20255084	None:intergenic
TGTCGGCTATTTGAATATAA+AGG	0.333196	3:+20256255	MsaT012176.1:CDS
AGATGCAAAGTTTGCTTGTT+CGG	0.333426	3:+20254859	MsaT012176.1:CDS
TCATCCTGCCTGTCAAGTTT+AGG	0.337518	3:-20256141	None:intergenic
TGTTCACAGGGTGCATGATA+TGG	0.338272	3:+20256173	MsaT012176.1:CDS
TCAAAACTTGCAGGCACAAC+TGG	0.340078	3:+20257496	MsaT012176.1:intron
ACTTTCCCATATTTCTACTT+TGG	0.350583	3:-20256620	None:intergenic
ATTGAGGTACATGCTCTTAA+TGG	0.357318	3:+20254647	MsaT012176.1:CDS
CAACTGGATTTCTTGCGAAC+TGG	0.365489	3:+20257512	MsaT012176.1:CDS
CCTACAGAAGCTGGTACATC+AGG	0.374818	3:+20256564	MsaT012176.1:CDS
AAAGAAGGCAAGATGGGAAA+TGG	0.381396	3:+20255014	MsaT012176.1:CDS
GGATCTTCTTGAATACTGTA+TGG	0.385556	3:-20256086	None:intergenic
AACTGTGGTGCTCTTACATT+AGG	0.389960	3:-20257917	None:intergenic
TCTTTGACAAATATGTTCAT+AGG	0.403324	3:+20255911	MsaT012176.1:CDS
AGGCAGAAGTGATGAATGTT+GGG	0.408722	3:+20256760	MsaT012176.1:CDS
AAGAAGGCAAGATGGGAAAT+GGG	0.409702	3:+20255015	MsaT012176.1:CDS
TGGTTTCGCATACAGCATTA+GGG	0.419366	3:+20257532	MsaT012176.1:CDS
ACAGTATGAAACCATCTTGC+AGG	0.419590	3:+20255960	MsaT012176.1:CDS
TCGAGCTCGTGGTCGGTTGA+AGG	0.420065	3:-20254912	None:intergenic
ACTTTGGGTATCTTCTTCAA+TGG	0.429012	3:-20256604	None:intergenic
CCAAGAATATGCTCGATCAA+TGG	0.430685	3:+20254781	MsaT012176.1:CDS
ACATCAAAGCATGCCTGGTT+TGG	0.435777	3:+20257409	MsaT012176.1:CDS
CAAAAGCAGATTCTGATACT+TGG	0.440513	3:+20256001	MsaT012176.1:CDS
GATACCCAAAGTAGAAATAT+GGG	0.442097	3:+20256615	MsaT012176.1:CDS
CAAGAATATGCTCGATCAAT+GGG	0.442288	3:+20254782	MsaT012176.1:CDS
AATTGACACTTTCAGATCGT+TGG	0.447716	3:-20255885	None:intergenic
TAAAGTTCGTAATTTGGGTC+TGG	0.453859	3:+20255189	MsaT012176.1:CDS
CAAGAATCTGAAAATTCAAC+TGG	0.454460	3:+20254944	MsaT012176.1:CDS
TCTTGAGCCACTTTCTGGTA+TGG	0.454926	3:+20254724	MsaT012176.1:CDS
ATGAAACCATCTTGCAGGAC+AGG	0.466060	3:+20255965	MsaT012176.1:CDS
ACCATCGTGGCTGGTTGGTA+TGG	0.466535	3:-20257375	None:intergenic
GGGAATCAACAGACTCTTCA+AGG	0.466578	3:+20257258	MsaT012176.1:CDS
ATTGACACTTTCAGATCGTT+GGG	0.467365	3:-20255884	None:intergenic
GAGGCAGAAGTGATGAATGT+TGG	0.469181	3:+20256759	MsaT012176.1:CDS
TGTTTGTTCACTTACTGCCT+GGG	0.477529	3:-20255083	None:intergenic
TTCATACCTGTCCTGCAAGA+TGG	0.481351	3:-20255971	None:intergenic
TGTTCACTTACTGCCTGGGC+TGG	0.481371	3:-20255079	None:intergenic
TTTGTATTCTGCAAACTGCC+TGG	0.482219	3:-20255128	None:intergenic
ATGAGACAAACGTTGTTCAC+AGG	0.483821	3:+20256160	MsaT012176.1:CDS
TTCTTTCTCATGTTCACCAG+TGG	0.488199	3:-20254528	None:intergenic
GATCTTCTTGAATACTGTAT+GGG	0.490261	3:-20256085	None:intergenic
ATGAACATATTTGTCAAAGA+AGG	0.490350	3:-20255908	None:intergenic
GACGCTCAAGCGTGTCTCGA+TGG	0.502862	3:-20257950	None:intergenic
AATCTGAACATATGCTGGCT+AGG	0.505661	3:+20256397	MsaT012176.1:CDS
CTGGTTGGTATGGAACTCAT+TGG	0.506086	3:-20257365	None:intergenic
TCTTTCTCATGTTCACCAGT+GGG	0.508767	3:-20254527	None:intergenic
AGAAGAATCTGAACATATGC+TGG	0.512248	3:+20256392	MsaT012176.1:CDS
GTATGGAATTCGAGTCTCAT+GGG	0.513881	3:+20254741	MsaT012176.1:CDS
AGCAAGAGTCCTACAGAAGC+TGG	0.514473	3:+20256555	MsaT012176.1:CDS
AAATTACGAACTTTATCGAA+TGG	0.514738	3:-20255181	None:intergenic
AACATTGCAATATCAACTGT+GGG	0.516629	3:-20254686	None:intergenic
ACTAGACAACATGCAACAAA+TGG	0.519807	3:+20256785	MsaT012176.1:CDS
GGACAAAAGATGCAAAAGTC+AGG	0.522255	3:+20256358	MsaT012176.1:CDS
CGTTTCAAAACATATTGAGA+TGG	0.523403	3:-20256333	None:intergenic
GAATGTATGCTGTGATGGCC+AGG	0.523831	3:+20255110	MsaT012176.1:CDS
TTATCTTTGTCGACATGCAT+TGG	0.525970	3:+20256278	MsaT012176.1:CDS
GGTATGGAATTCGAGTCTCA+TGG	0.526936	3:+20254740	MsaT012176.1:CDS
ATTGAGATGGGAACACTGAA+TGG	0.527069	3:-20256320	None:intergenic
GGTGCACATCAAAGCATGCC+TGG	0.527959	3:+20257404	MsaT012176.1:CDS
AAAATTGCACAATGGAGGTA+TGG	0.528216	3:+20254598	MsaT012176.1:CDS
CTTGTCATCCTAAACTTGAC+AGG	0.529447	3:+20256133	MsaT012176.1:CDS
TGTTGGATTGTAGGTGAACT+CGG	0.535610	3:+20256737	MsaT012176.1:intron
TCATCCTAAACTTGACAGGC+AGG	0.538131	3:+20256137	MsaT012176.1:CDS
GCAGAATACAAAACTGTGGT+TGG	0.538665	3:+20255139	MsaT012176.1:CDS
TGCATTCCATGCACTAAATG+AGG	0.540583	3:+20256500	MsaT012176.1:CDS
AGGTATGGACGACAGACATA+TGG	0.540778	3:+20254613	MsaT012176.1:CDS
GTTTCAAAACATATTGAGAT+GGG	0.541753	3:-20256332	None:intergenic
TCATCATGGATATAGATCTC+AGG	0.543112	3:+20254501	None:intergenic
GCTGGTACATCAGGGGCTCA+TGG	0.543275	3:+20256573	MsaT012176.1:CDS
TCTTTGTCGACATGCATTGG+TGG	0.544560	3:+20256281	MsaT012176.1:CDS
TTCTGATACTTGGAATAAAG+TGG	0.545659	3:+20256011	MsaT012176.1:CDS
ATCTGAACATATGCTGGCTA+GGG	0.545736	3:+20256398	MsaT012176.1:CDS
AGCTGAGATTATTGGTGCAG+TGG	0.546997	3:+20256110	MsaT012176.1:CDS
CCAACACGTGATGATTACTA+CGG	0.549419	3:+20257237	MsaT012176.1:CDS
TGCATCTATAAATTCTCTTG+AGG	0.551832	3:-20254843	None:intergenic
ACCATAATAACCATCGTGGC+TGG	0.555757	3:-20257384	None:intergenic
TGCTTGTTCTGTCGAGCTCG+TGG	0.562836	3:-20254923	None:intergenic
TCGAATTCCATACCAGAAAG+TGG	0.566016	3:-20254731	None:intergenic
TGTGTGCCTCATTTAGTGCA+TGG	0.566180	3:-20256506	None:intergenic
TCACTAAAATTGAGCGAAGA+GGG	0.566685	3:+20256450	MsaT012176.1:CDS
GGTTATTCATAGCTTTGTGA+AGG	0.566920	3:+20255036	MsaT012176.1:CDS
GACAGACATATGGTTGAAGC+TGG	0.568268	3:+20254623	MsaT012176.1:CDS
TTTGGGTCTGGAGTTTGGGG+AGG	0.569035	3:+20255201	MsaT012176.1:CDS
TGTTCTGTCGAGCTCGTGGT+CGG	0.571836	3:-20254919	None:intergenic
TTTAACAAAATCTTGCACAT+AGG	0.572860	3:-20255938	None:intergenic
TATGGTTGAAGCTGGTATTG+AGG	0.573490	3:+20254631	MsaT012176.1:CDS
CTGCACCAATAATCTCAGCT+TGG	0.574589	3:-20256107	None:intergenic
AACTACACTTGATAACATGT+TGG	0.575276	3:+20254565	MsaT012176.1:CDS
TCAACAGACTCTTCAAGGAC+TGG	0.577433	3:+20257263	MsaT012176.1:CDS
CTACAGAAGCTGGTACATCA+GGG	0.582637	3:+20256565	MsaT012176.1:CDS
CTTTAGAAAAGAGTGTTGCA+GGG	0.584026	3:+20256055	MsaT012176.1:CDS
CAGAGGTGTCTTGTATATGT+CGG	0.585513	3:+20256238	MsaT012176.1:CDS
AAGAAGAATGTATGCTGTGA+TGG	0.589551	3:+20255105	MsaT012176.1:CDS
AAACATTGCAATATCAACTG+TGG	0.593917	3:-20254687	None:intergenic
TAATTTGGGTCTGGAGTTTG+GGG	0.596349	3:+20255198	MsaT012176.1:CDS
CAACACGTGATGATTACTAC+GGG	0.600808	3:+20257238	MsaT012176.1:CDS
TGAGACAAACGTTGTTCACA+GGG	0.601018	3:+20256161	MsaT012176.1:CDS
TGCAAAAGTCAGGAATGTCA+CGG	0.602820	3:+20256368	MsaT012176.1:CDS
GCAAAAGTCAGGAATGTCAC+GGG	0.606359	3:+20256369	MsaT012176.1:CDS
TAATAACCATCGTGGCTGGT+TGG	0.607504	3:-20257380	None:intergenic
CCTGATGTACCAGCTTCTGT+AGG	0.610024	3:-20256564	None:intergenic
GCAGGACAGGTATGAAGAGG+AGG	0.615423	3:+20255978	MsaT012176.1:CDS
ACACTTGATAACATGTTGGA+AGG	0.620645	3:+20254569	MsaT012176.1:CDS
GAGGTACATGCTCTTAATGG+TGG	0.620892	3:+20254650	MsaT012176.1:CDS
CATTGCAATATCAACTGTGG+GGG	0.621147	3:-20254684	None:intergenic
ATCACTAAAATTGAGCGAAG+AGG	0.621826	3:+20256449	MsaT012176.1:CDS
GGTGAATGAAGTAAAATCAG+AGG	0.623034	3:+20256221	MsaT012176.1:CDS
ATAGATCTCAGGTTACCCAC+TGG	0.627040	3:+20254512	MsaT012176.1:CDS
CTTGCAGGACAGGTATGAAG+AGG	0.628672	3:+20255975	MsaT012176.1:CDS
TCCATACCAACCAGCCACGA+TGG	0.649328	3:+20257374	MsaT012176.1:CDS
CCGTAGTAATCATCACGTGT+TGG	0.653378	3:-20257237	None:intergenic
TTAGTGAAACATACCAAACC+AGG	0.659731	3:-20257422	None:intergenic
GGCAGAAGTGATGAATGTTG+GGG	0.664453	3:+20256761	MsaT012176.1:CDS
TACAGAAGCTGGTACATCAG+GGG	0.668012	3:+20256566	MsaT012176.1:CDS
GACACTTTCAGATCGTTGGG+CGG	0.679327	3:-20255881	None:intergenic
TATGGAATTCGAGTCTCATG+GGG	0.685488	3:+20254742	MsaT012176.1:CDS
GATGGATCTTCGTGTAACTG+TGG	0.687451	3:-20257932	None:intergenic
ACATTGCAATATCAACTGTG+GGG	0.697658	3:-20254685	None:intergenic
GTTTGCAGAATACAAAACTG+TGG	0.699066	3:+20255135	MsaT012176.1:CDS
TGGATTGTAGGTGAACTCGG+AGG	0.756855	3:+20256740	MsaT012176.1:intron
GTGCACCATAATAACCATCG+TGG	0.809451	3:-20257388	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	20254506	20257967	20254506	ID=MsaG012176
Chr3	mRNA	20254506	20257967	20254506	ID=MsaT012176.1;Parent=MsaG012176
Chr3	exon	20254506	20255259	20254506	ID=MsaT012176.1.exon1;Parent=MsaT012176.1
Chr3	CDS	20254506	20255259	20254506	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20255869	20256677	20255869	ID=MsaT012176.1.exon2;Parent=MsaT012176.1
Chr3	CDS	20255869	20256677	20255869	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20256750	20256806	20256750	ID=MsaT012176.1.exon3;Parent=MsaT012176.1
Chr3	CDS	20256750	20256806	20256750	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20257222	20257284	20257222	ID=MsaT012176.1.exon4;Parent=MsaT012176.1
Chr3	CDS	20257222	20257284	20257222	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20257362	20257430	20257362	ID=MsaT012176.1.exon5;Parent=MsaT012176.1
Chr3	CDS	20257362	20257430	20257362	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20257509	20257553	20257509	ID=MsaT012176.1.exon6;Parent=MsaT012176.1
Chr3	CDS	20257509	20257553	20257509	ID=cds.MsaT012176.1;Parent=MsaT012176.1
Chr3	exon	20257911	20257967	20257911	ID=MsaT012176.1.exon7;Parent=MsaT012176.1
Chr3	CDS	20257911	20257967	20257911	ID=cds.MsaT012176.1;Parent=MsaT012176.1

Gene Sequence

>MsaT012176.1

ATGGATATAGATCTCAGGTTACCCACTGGTGAACATGAGAAAGAAGAAGAAGAAGAAACAACTACACTTGATAACATGTTGGAAGGTGAAGAAAAATTGCACAATGGAGGTATGGACGACAGACATATGGTTGAAGCTGGTATTGAGGTACATGCTCTTAATGGTGGAGATCTGAATTCCCCCACAGTTGATATTGCAATGTTTAAGGAAGATACAAATCTTGAGCCACTTTCTGGTATGGAATTCGAGTCTCATGGGGAAGCTTATTCTTTCTACCAAGAATATGCTCGATCAATGGGATTCAACACTGCAATACAAAATAGTCGCCGTTCAAAAACCTCAAGAGAATTTATAGATGCAAAGTTTGCTTGTTCGGATATGGAACAAAAGCGAGAAATCGATAAATCCTTCAACCGACCACGAGCTCGACAGAACAAGCAAGAATCTGAAAATTCAACTGGTCGAAGATCTTGCTCTAAGACTGATTGTAAAGCAAGCATGCATGTAAAAAGAAGGCAAGATGGGAAATGGGTTATTCATAGCTTTGTGAAGGAGCATAATCACGAACTTTTACCAGCCCAGGCAGTAAGTGAACAAACAAGAAGAATGTATGCTGTGATGGCCAGGCAGTTTGCAGAATACAAAACTGTGGTTGGTATCAAGAATGAAAAGAATCCATTCGATAAAGTTCGTAATTTGGGTCTGGAGTTTGGGGAGGCTAAACTTATGCTTGATTTTTTTATTCAGATGCAAAGTGGCATGTCTACCGCCCAACGATCTGAAAGTGTCAATTCCTTCTTTGACAAATATGTTCATAGGAAGACCTATGTGCAAGATTTTGTTAAACAGTATGAAACCATCTTGCAGGACAGGTATGAAGAGGAGGCAAAAGCAGATTCTGATACTTGGAATAAAGTGGCAACATTGAAAACTCCTTCACCTTTAGAAAAGAGTGTTGCAGGGATTTGCACCCATACAGTATTCAAGAAGATCCAAGCTGAGATTATTGGTGCAGTGGCTTGTCATCCTAAACTTGACAGGCAGGATGAGACAAACGTTGTTCACAGGGTGCATGATATGGAAATAAACAGAGACTTTTTTGTCGTGGTGAATGAAGTAAAATCAGAGGTGTCTTGTATATGTCGGCTATTTGAATATAAAGGTTATCTTTGTCGACATGCATTGGTGGTTCTTCAATATTCTGGCCATTCAGTGTTCCCATCTCAATATGTTTTGAAACGGTGGACAAAAGATGCAAAAGTCAGGAATGTCACGGGAGAAGAATCTGAACATATGCTGGCTAGGGTGCAACGATACAATGATTTATGTCATCGATCACTAAAATTGAGCGAAGAGGGATCATTGTCTCAAGATAGTTATAGTATTGCATTCCATGCACTAAATGAGGCACACAAAAGTTGTGTGAGTGTTAACAATTCTAGCAAGAGTCCTACAGAAGCTGGTACATCAGGGGCTCATGGTCAGCTTTCCATTGAAGAAGATACCCAAAGTAGAAATATGGGAAAGTCAAACAAAAAGAAGAATCCAACTAAAAAGAAAAAGGTGAACTCGGAGGCAGAAGTGATGAATGTTGGGGCACTAGACAACATGCAACAAATGTTGAACTTAATGGGACCAACACGTGATGATTACTACGGGAATCAACAGACTCTTCAAGGACTGGGACCAATGAGTTCCATACCAACCAGCCACGATGGTTATTATGGTGCACATCAAAGCATGCCTGGTTTGGCACAACTGGATTTCTTGCGAACTGGTTTCGCATACAGCATTAGGGATGAACCTAATGTAAGAGCACCACAGTTACACGAAGATCCATCGAGACACGCTTGA

Protein sequence

>MsaT012176.1

MDIDLRLPTGEHEKEEEEETTTLDNMLEGEEKLHNGGMDDRHMVEAGIEVHALNGGDLNSPTVDIAMFKEDTNLEPLSGMEFESHGEAYSFYQEYARSMGFNTAIQNSRRSKTSREFIDAKFACSDMEQKREIDKSFNRPRARQNKQESENSTGRRSCSKTDCKASMHVKRRQDGKWVIHSFVKEHNHELLPAQAVSEQTRRMYAVMARQFAEYKTVVGIKNEKNPFDKVRNLGLEFGEAKLMLDFFIQMQSGMSTAQRSESVNSFFDKYVHRKTYVQDFVKQYETILQDRYEEEAKADSDTWNKVATLKTPSPLEKSVAGICTHTVFKKIQAEIIGAVACHPKLDRQDETNVVHRVHDMEINRDFFVVVNEVKSEVSCICRLFEYKGYLCRHALVVLQYSGHSVFPSQYVLKRWTKDAKVRNVTGEESEHMLARVQRYNDLCHRSLKLSEEGSLSQDSYSIAFHALNEAHKSCVSVNNSSKSPTEAGTSGAHGQLSIEEDTQSRNMGKSNKKKNPTKKKKVNSEAEVMNVGALDNMQQMLNLMGPTRDDYYGNQQTLQGLGPMSSIPTSHDGYYGAHQSMPGLAQLDFLRTGFAYSIRDEPNVRAPQLHEDPSRHA*