Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG012186 | AES61431.2 | 92.887 | 239 | 17 | 0 | 1 | 239 | 1 | 239 | 2.64e-165 | 468 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG012186 | G7I8T1 | 92.887 | 239 | 17 | 0 | 1 | 239 | 1 | 239 | 1.26e-165 | 468 |
Gene ID | Type | Classification |
---|---|---|
MsaG012186 | TF | FAR1 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG001001 | MsaG012186 | 0.827767 | 2.330174e-54 | 6.330683e-52 |
MsaG003913 | MsaG012186 | 0.805211 | 2.517849e-49 | 3.835962e-47 |
MsaG004012 | MsaG012186 | 0.806028 | 1.699250e-49 | 2.638718e-47 |
MsaG004353 | MsaG012186 | 0.805030 | 2.746279e-49 | 4.166565e-47 |
MsaG004442 | MsaG012186 | 0.806929 | 1.099486e-49 | 1.744075e-47 |
MsaG004479 | MsaG012186 | 0.800488 | 2.354452e-48 | 3.218605e-46 |
MsaG004793 | MsaG012186 | 0.806070 | 1.665824e-49 | 2.589421e-47 |
MsaG004798 | MsaG012186 | 0.815692 | 1.405988e-51 | 2.765980e-49 |
MsaG004929 | MsaG012186 | 0.808929 | 4.146260e-50 | 6.900198e-48 |
MsaG005698 | MsaG012186 | 0.826474 | 4.735090e-54 | 1.240829e-51 |
MsaG006133 | MsaG012186 | 0.864325 | 2.627625e-64 | 2.416613e-61 |
MsaG007145 | MsaG012186 | 0.807054 | 1.034755e-49 | 1.646252e-47 |
MsaG009007 | MsaG012186 | 0.814877 | 2.129519e-51 | 4.103843e-49 |
MsaG009265 | MsaG012186 | 0.819760 | 1.716414e-52 | 3.750794e-50 |
MsaG009703 | MsaG012186 | 0.809746 | 2.775838e-50 | 4.711340e-48 |
MsaG012186 | MsaG012187 | 0.824933 | 1.094007e-53 | 2.747038e-51 |
MsaG012186 | MsaG012304 | 0.804261 | 3.967118e-49 | 5.912281e-47 |
MsaG012186 | MsaG013374 | 0.828811 | 1.308896e-54 | 3.661620e-52 |
MsaG012186 | MsaG015082 | 0.816164 | 1.104824e-51 | 2.199714e-49 |
MsaG012186 | MsaG015176 | 0.800982 | 1.868476e-48 | 2.582902e-46 |
MsaG012186 | MsaG015239 | 0.812681 | 6.451699e-51 | 1.176717e-48 |
MsaG012186 | MsaG016293 | 0.824541 | 1.352039e-53 | 3.358741e-51 |
MsaG012186 | MsaG017173 | 0.815689 | 1.408742e-51 | 2.771122e-49 |
MsaG012186 | MsaG017543 | 0.802122 | 1.093926e-48 | 1.551785e-46 |
MsaG012186 | MsaG017795 | 0.806677 | 1.242014e-49 | 1.958583e-47 |
MsaG012186 | MsaG018111 | 0.832689 | 1.483056e-55 | 4.639018e-53 |
MsaG012186 | MsaG018593 | 0.832960 | 1.270793e-55 | 4.006783e-53 |
MsaG012186 | MsaG018741 | 0.807841 | 7.057199e-50 | 1.144104e-47 |
MsaG012186 | MsaG019265 | 0.804144 | 4.193918e-49 | 6.233364e-47 |
MsaG012186 | MsaG020487 | 0.801613 | 1.389676e-48 | 1.948697e-46 |
MsaG012186 | MsaG020731 | 0.825116 | 9.909496e-54 | 2.500700e-51 |
MsaG012186 | MsaG020870 | 0.849730 | 5.094795e-60 | 2.736007e-57 |
MsaG012186 | MsaG020963 | 0.830277 | 5.781783e-55 | 1.686623e-52 |
MsaG012186 | MsaG021238 | 0.819994 | 1.518545e-52 | 3.339323e-50 |
MsaG012186 | MsaG021448 | 0.802538 | 8.987461e-49 | 1.287192e-46 |
MsaG012186 | MsaG023607 | 0.828624 | 1.451414e-54 | 4.039264e-52 |
MsaG012186 | MsaG024972 | 0.809314 | 3.433011e-50 | 5.765947e-48 |
MsaG012186 | MsaG025428 | 0.859370 | 8.496804e-63 | 6.450227e-60 |
MsaG012186 | MsaG025722 | 0.801896 | 1.216868e-48 | 1.717405e-46 |
MsaG012186 | MsaG026841 | 0.804117 | 4.247909e-49 | 6.309694e-47 |
MsaG012186 | MsaG026863 | 0.800227 | 2.659264e-48 | 3.613892e-46 |
MsaG012186 | MsaG027005 | 0.834699 | 4.692007e-56 | 1.557578e-53 |
MsaG012186 | MsaG027361 | 0.800873 | 1.967087e-48 | 2.712498e-46 |
MsaG012186 | MsaG027933 | 0.848398 | 1.190496e-59 | 6.109386e-57 |
MsaG012186 | MsaG028001 | 0.804239 | 4.009010e-49 | 5.971620e-47 |
MsaG012186 | MsaG028498 | 0.814968 | 2.033579e-51 | 3.927961e-49 |
MsaG012186 | MsaG028951 | 0.850478 | 3.153677e-60 | 1.737508e-57 |
MsaG012186 | MsaG029481 | 0.800450 | 2.396962e-48 | 3.273930e-46 |
MsaG012186 | MsaG029519 | 0.863007 | 6.713941e-64 | 5.861579e-61 |
MsaG012186 | MsaG029763 | 0.800236 | 2.647834e-48 | 3.599092e-46 |
MsaG012186 | MsaG030955 | 0.815351 | 1.673189e-51 | 3.263328e-49 |
MsaG012186 | MsaG032068 | 0.813100 | 5.227706e-51 | 9.634998e-49 |
MsaG012186 | MsaG033030 | 0.801152 | 1.725508e-48 | 2.394424e-46 |
MsaG012186 | MsaG034079 | 0.833643 | 8.603846e-56 | 2.767863e-53 |
MsaG012186 | MsaG039196 | 0.836695 | 1.472888e-56 | 5.191238e-54 |
MsaG012186 | MsaG039924 | 0.808666 | 4.716896e-50 | 7.800471e-48 |
MsaG012186 | MsaG041905 | 0.804699 | 3.218229e-49 | 4.844908e-47 |
MsaG012186 | MsaG044218 | 0.824156 | 1.664454e-53 | 4.091460e-51 |
MsaG012186 | MsaG044885 | 0.829107 | 1.110369e-54 | 3.132324e-52 |
MsaG012186 | MsaG045072 | 0.802181 | 1.063815e-48 | 1.511152e-46 |
MsaG012186 | MsaG045225 | 0.819070 | 2.461758e-52 | 5.283243e-50 |
MsaG012186 | MsaG046313 | 0.804456 | 3.614175e-49 | 5.410471e-47 |
MsaG012186 | MsaG046546 | 0.821046 | 8.728661e-53 | 1.973681e-50 |
MsaG012186 | MsaG000630 | 0.811607 | 1.103786e-50 | 1.960450e-48 |
MsaG012186 | MsaG003742 | 0.817716 | 4.971058e-52 | 1.029965e-49 |
MsaG012186 | MsaG014180 | 0.812671 | 6.484845e-51 | 1.182452e-48 |
MsaG012186 | MsaG019831 | 0.804434 | 3.651864e-49 | 5.464197e-47 |
MsaG012186 | MsaG028462 | 0.810075 | 2.359368e-50 | 4.036516e-48 |
MsaG012186 | MsaG030315 | 0.805029 | 2.746692e-49 | 4.167130e-47 |
MsaG012186 | MsaG030924 | 0.812671 | 6.484845e-51 | 1.182452e-48 |
MsaG012186 | MsaG034181 | 0.800827 | 2.009877e-48 | 2.768623e-46 |
MsaG012186 | MsaG032695 | 0.806275 | 1.508686e-49 | 2.356676e-47 |
MsaG012186 | MsaG031291 | 0.824120 | 1.696802e-53 | 4.167035e-51 |
MsaG012186 | MsaG036873 | 0.840184 | 1.872616e-57 | 7.352393e-55 |
MsaG012186 | MsaG035287 | 0.800848 | 1.989557e-48 | 2.741967e-46 |
MsaG012186 | MsaG037156 | 0.843214 | 2.998761e-58 | 1.296449e-55 |
MsaG012186 | MsaG036524 | 0.813556 | 4.155359e-51 | 7.746639e-49 |
MsaG012186 | MsaG038711 | 0.823473 | 2.400886e-53 | 5.793523e-51 |
MsaG012186 | MsaG036751 | 0.857086 | 4.034711e-62 | 2.812197e-59 |
MsaG012186 | MsaG042234 | 0.812399 | 7.431957e-51 | 1.346049e-48 |
MsaG012186 | MsaG043146 | 0.800232 | 2.652570e-48 | 3.605220e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG012186 | MtrunA17_Chr1g0172971 | 75.000 | 132 | 18 | 1 | 1 | 132 | 1 | 117 | 6.51e-62 | 202 |
MsaG012186 | MtrunA17_Chr6g0475591 | 86.111 | 36 | 5 | 0 | 204 | 239 | 1 | 36 | 9.67e-17 | 72.8 |
MsaG012186 | MtrunA17_Chr1g0202341 | 29.730 | 185 | 106 | 6 | 50 | 232 | 50 | 212 | 6.68e-14 | 70.9 |
MsaG012186 | MtrunA17_Chr7g0258861 | 27.041 | 196 | 121 | 5 | 46 | 238 | 2 | 178 | 4.26e-12 | 64.7 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 37 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TCTGCTGTTGGCCTGTTGTT+TGG | 0.302805 | 3:+20424534 | None:intergenic |
TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 3:+20424343 | None:intergenic |
GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 3:-20424268 | MsaT012186.1:CDS |
CTAAGGTAACGCGAATGCTT+TGG | 0.399481 | 3:+20424360 | None:intergenic |
CGTTATGTTTGTAACAAAGA+AGG | 0.427485 | 3:-20424582 | MsaT012186.1:CDS |
GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 3:-20424255 | MsaT012186.1:CDS |
CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 3:-20424330 | MsaT012186.1:CDS |
TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 3:+20424745 | None:intergenic |
TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 3:+20424744 | None:intergenic |
GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 3:-20424465 | MsaT012186.1:CDS |
ATTTACTTAATCCAAACAAC+AGG | 0.452259 | 3:-20424545 | MsaT012186.1:CDS |
ATGTGTATAAGATATATAGA+TGG | 0.452819 | 3:-20424413 | MsaT012186.1:CDS |
GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 3:-20424300 | MsaT012186.1:CDS |
AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 3:-20424267 | MsaT012186.1:CDS |
ACAAAACAAGAAATTTGATA+AGG | 0.468895 | 3:-20424616 | MsaT012186.1:CDS |
TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 3:+20424216 | None:intergenic |
GAATGCTTTGGTTTATGTAA+TGG | 0.496214 | 3:+20424372 | None:intergenic |
CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 3:-20424746 | MsaT012186.1:CDS |
TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 3:+20424471 | None:intergenic |
AGCTGGAATATATTTGAAGC+TGG | 0.507410 | 3:-20424448 | MsaT012186.1:CDS |
GGTAATTATGCATATAACAC+TGG | 0.523547 | 3:-20424678 | MsaT012186.1:CDS |
TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 3:-20424571 | MsaT012186.1:CDS |
TAAGATATATAGATGGGATG+TGG | 0.528498 | 3:-20424406 | MsaT012186.1:CDS |
CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 3:+20424746 | None:intergenic |
AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 3:-20424570 | MsaT012186.1:CDS |
GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 3:-20424252 | MsaT012186.1:CDS |
TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 3:-20424261 | MsaT012186.1:CDS |
ATGACAATTCACAAAAGAGA+TGG | 0.583940 | 3:-20424183 | MsaT012186.1:CDS |
TTCACAAAAGAGATGGTGGT+TGG | 0.595505 | 3:-20424176 | MsaT012186.1:CDS |
TGTGTATAAGATATATAGAT+GGG | 0.601002 | 3:-20424412 | MsaT012186.1:CDS |
ACCAGTGGACTGTCTGCTGT+TGG | 0.602965 | 3:+20424522 | None:intergenic |
TCACTGATTCATGCTGAGAG+TGG | 0.606950 | 3:-20424825 | MsaT012186.1:CDS |
GCCAACAGCAGACAGTCCAC+TGG | 0.611268 | 3:-20424523 | MsaT012186.1:CDS |
ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 3:-20424870 | MsaT012186.1:CDS |
ACAATTCACAAAAGAGATGG+TGG | 0.617339 | 3:-20424180 | MsaT012186.1:CDS |
TTCACAAACTCAATAACCAG+TGG | 0.633415 | 3:+20424507 | None:intergenic |
GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 3:-20424247 | MsaT012186.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 20424173 | 20424901 | 20424173 | ID=MsaG012186 |
Chr3 | mRNA | 20424173 | 20424901 | 20424173 | ID=MsaT012186.1;Parent=MsaG012186 |
Chr3 | exon | 20424173 | 20424901 | 20424173 | ID=MsaT012186.1.exon1;Parent=MsaT012186.1 |
Chr3 | CDS | 20424173 | 20424901 | 20424173 | ID=cds.MsaT012186.1;Parent=MsaT012186.1 |
Gene Sequence |
Protein sequence |