AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012186


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012186 MtrunA17_Chr1g0172971 75.000 132 18 1 1 132 1 117 6.51e-62 202
MsaG012186 MtrunA17_Chr6g0475591 86.111 36 5 0 204 239 1 36 9.67e-17 72.8
MsaG012186 MtrunA17_Chr1g0202341 29.730 185 106 6 50 232 50 212 6.68e-14 70.9
MsaG012186 MtrunA17_Chr7g0258861 27.041 196 121 5 46 238 2 178 4.26e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCTGTTGGCCTGTTGTT+TGG 0.302805 3:+20424534 None:intergenic
TTACTTCTCGAAATGATCTA+AGG 0.311482 3:+20424343 None:intergenic
GAGAACGTCTTATGAAGTTA+TGG 0.394356 3:-20424268 MsaT012186.1:CDS
CTAAGGTAACGCGAATGCTT+TGG 0.399481 3:+20424360 None:intergenic
CGTTATGTTTGTAACAAAGA+AGG 0.427485 3:-20424582 MsaT012186.1:CDS
GAAGTTATGGGTCAAGGAGT+TGG 0.428685 3:-20424255 MsaT012186.1:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 3:-20424330 MsaT012186.1:CDS
TCCATGATTCTTTATTGCTA+GGG 0.444266 3:+20424745 None:intergenic
TTCCATGATTCTTTATTGCT+AGG 0.445442 3:+20424744 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 3:-20424465 MsaT012186.1:CDS
ATTTACTTAATCCAAACAAC+AGG 0.452259 3:-20424545 MsaT012186.1:CDS
ATGTGTATAAGATATATAGA+TGG 0.452819 3:-20424413 MsaT012186.1:CDS
GCTCTTATAAATTCTAAAGC+TGG 0.462891 3:-20424300 MsaT012186.1:CDS
AGAACGTCTTATGAAGTTAT+GGG 0.466325 3:-20424267 MsaT012186.1:CDS
ACAAAACAAGAAATTTGATA+AGG 0.468895 3:-20424616 MsaT012186.1:CDS
TTTAAGTCTGAAAACCGAAA+AGG 0.483010 3:+20424216 None:intergenic
GAATGCTTTGGTTTATGTAA+TGG 0.496214 3:+20424372 None:intergenic
CCCCTAGCAATAAAGAATCA+TGG 0.497001 3:-20424746 MsaT012186.1:CDS
TTACAACCAACCCTTTCCTC+AGG 0.505082 3:+20424471 None:intergenic
AGCTGGAATATATTTGAAGC+TGG 0.507410 3:-20424448 MsaT012186.1:CDS
GGTAATTATGCATATAACAC+TGG 0.523547 3:-20424678 MsaT012186.1:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.525236 3:-20424571 MsaT012186.1:CDS
TAAGATATATAGATGGGATG+TGG 0.528498 3:-20424406 MsaT012186.1:CDS
CCATGATTCTTTATTGCTAG+GGG 0.537011 3:+20424746 None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 3:-20424570 MsaT012186.1:CDS
GTTATGGGTCAAGGAGTTGG+AGG 0.578191 3:-20424252 MsaT012186.1:CDS
TCTTATGAAGTTATGGGTCA+AGG 0.580120 3:-20424261 MsaT012186.1:CDS
ATGACAATTCACAAAAGAGA+TGG 0.583940 3:-20424183 MsaT012186.1:CDS
TTCACAAAAGAGATGGTGGT+TGG 0.595505 3:-20424176 MsaT012186.1:CDS
TGTGTATAAGATATATAGAT+GGG 0.601002 3:-20424412 MsaT012186.1:CDS
ACCAGTGGACTGTCTGCTGT+TGG 0.602965 3:+20424522 None:intergenic
TCACTGATTCATGCTGAGAG+TGG 0.606950 3:-20424825 MsaT012186.1:CDS
GCCAACAGCAGACAGTCCAC+TGG 0.611268 3:-20424523 MsaT012186.1:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 3:-20424870 MsaT012186.1:CDS
ACAATTCACAAAAGAGATGG+TGG 0.617339 3:-20424180 MsaT012186.1:CDS
TTCACAAACTCAATAACCAG+TGG 0.633415 3:+20424507 None:intergenic
GGGTCAAGGAGTTGGAGGCA+CGG 0.637387 3:-20424247 MsaT012186.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 20424173 20424901 20424173 ID=MsaG012186
Chr3 mRNA 20424173 20424901 20424173 ID=MsaT012186.1;Parent=MsaG012186
Chr3 exon 20424173 20424901 20424173 ID=MsaT012186.1.exon1;Parent=MsaT012186.1
Chr3 CDS 20424173 20424901 20424173 ID=cds.MsaT012186.1;Parent=MsaT012186.1
Gene Sequence

>MsaT012186.1

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAACTGAGTGTTGAAACTTCATCACTGATTCATGCTGAGAGTGGACAATTGAGTGTTGAAAGTTCTTCAATGATTCATGCTGACAGTTCTCAATGTTACTCCCCTAGCAATAAAGAATCATGGAAGTCTTTAGTCTTTCAATCTATTAAAGAGATTGAAGATTTTTATGGTAATTATGCATATAACACTGGATTTTCTATTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAACAAGAAATTTGATAAGGTACATTATTTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAATTTACTTAATCCAAACAACAGGCCAACAGCAGACAGTCCACTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCTGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGCTGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGATCACTGTCATCCATTACATAAACCAAAGCATTCGCGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGCTGGAATGTCGATGAGAACGTCTTATGAAGTTATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGGTTTTCAGACTTAAAGAACTATCTAATGACAATTCACAAAAGAGATGGTGGTTGGTGA

Protein sequence

>MsaT012186.1

MDLISTSSESGQLSVETSSLIHAESGQLSVESSSMIHADSSQCYSPSNKESWKSLVFQSIKEIEDFYGNYAYNTGFSIRTMLKSKNNSQNKKFDKVHYLRYVCNKEGFKKGNLLNPNNRPTADSPLVIEFVKVKEKPEERVGCKAGIYLKLDEVLNVYKIYRWDVDHCHPLHKPKHSRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTIHKRDGGW*