AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012209


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012209 MtrunA17_Chr3g0079841 94.416 197 11 0 17 213 26 222 8.15e-135 380
MsaG012209 MtrunA17_Chr1g0213311 46.667 210 94 5 12 205 15 222 4.14e-51 171
MsaG012209 MtrunA17_Chr3g0143581 49.286 140 69 1 1 138 1 140 1.04e-41 140
MsaG012209 MtrunA17_Chr2g0320431 83.871 62 10 0 159 220 215 276 1.32e-25 100
MsaG012209 MtrunA17_Chr7g0214501 41.176 119 39 1 83 201 3 90 1.24e-17 80.1
MsaG012209 MtrunA17_Chr7g0214491 56.667 60 26 0 21 80 26 85 1.01e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012209 AT3G13350.1 50.000 186 77 3 25 208 34 205 3.11e-52 171
MsaG012209 AT1G55650.1 46.277 188 81 4 26 209 29 200 2.69e-51 169
MsaG012209 AT1G04880.1 44.103 195 101 3 16 202 14 208 9.41e-48 163
MsaG012209 AT1G76110.1 47.027 185 91 4 21 201 28 209 1.97e-45 154

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGTGATGCTAACCTCTTC+TGG 0.176030 3:+20828061 MsaT012209.1:CDS
GGAGCAATTAGTAGGAAAAT+TGG 0.205100 3:+20830841 MsaT012209.1:CDS
GTTCAGTCTCACGCGGATTT+TGG 0.242316 3:-20827993 None:intergenic
TGTTTCAAGCAATGCATCTA+AGG 0.259440 3:+20830665 MsaT012209.1:CDS
TATCAATGATTCACCTGTTC+AGG 0.286987 3:+20829494 MsaT012209.1:CDS
AGGTAATTGTAGATCGTAAA+TGG 0.317695 3:+20829196 MsaT012209.1:intron
TGTGTAGGATTCCTACTATA+GGG 0.338046 3:+20828885 MsaT012209.1:intron
AGTGTCTGTGCTGGACTAAT+TGG 0.354657 3:-20830543 None:intergenic
GAGCAATTAGTAGGAAAATT+GGG 0.369568 3:+20830842 MsaT012209.1:CDS
CATAACAAGCGCATCATTCA+TGG 0.386953 3:+20829254 MsaT012209.1:CDS
CGTATTGGTTGTGAAGCTAT+TGG 0.406695 3:-20829460 None:intergenic
TTCATTGACTTACCCTGAAC+AGG 0.413930 3:-20829507 None:intergenic
TTGTGTAGGATTCCTACTAT+AGG 0.432421 3:+20828884 MsaT012209.1:intron
GTGATAAAGCAACGATAGAT+AGG 0.439414 3:-20829286 None:intergenic
TCGTGATGCTAACCTCTTCT+GGG 0.445073 3:+20828062 MsaT012209.1:CDS
CATGAATGATGCGCTTGTTA+TGG 0.445119 3:-20829253 None:intergenic
GAGTAGAGAGCGGTTGGAGG+AGG 0.454942 3:-20828030 None:intergenic
TCTAAGGGTTCTTCTATTGA+AGG 0.459768 3:+20830681 MsaT012209.1:CDS
ACAATTGACATGAAATTTGA+TGG 0.468681 3:+20830585 MsaT012209.1:CDS
ATATATAGTTACGGTGGACC+TGG 0.474943 3:+20830611 MsaT012209.1:CDS
GATAGCAGCACCGTCCGTAT+TGG 0.475279 3:-20829475 None:intergenic
ATCTCGTGGTGGTATTGAAA+AGG 0.485522 3:+20828949 MsaT012209.1:CDS
CTTCACTAACCAGGTGTTGA+GGG 0.490947 3:-20829345 None:intergenic
CTCTATCCAGATGCTTTGAG+TGG 0.513745 3:+20829429 MsaT012209.1:intron
CATTGCTTGAAACATGATAC+AGG 0.516873 3:-20830656 None:intergenic
ATCAATGATTCACCTGTTCA+GGG 0.519797 3:+20829495 MsaT012209.1:CDS
TTCCTACTAATTGCTCCTTG+TGG 0.533197 3:-20830835 None:intergenic
AGGTCAGAGTAGAGAGCGGT+TGG 0.533695 3:-20828036 None:intergenic
TCAGAGTAGAGAGCGGTTGG+AGG 0.537076 3:-20828033 None:intergenic
GGAAAATTGGGTTTGCGTGG+AGG 0.541228 3:+20830854 MsaT012209.1:CDS
CAATAGCTTCACAACCAATA+CGG 0.543956 3:+20829461 MsaT012209.1:CDS
TGATGGCGGATATATAGTTA+CGG 0.545231 3:+20830602 MsaT012209.1:CDS
CAAGTCCCTCCCTCAACACC+TGG 0.550783 3:+20829336 MsaT012209.1:CDS
ATTAGTCCAGCACAGACACT+TGG 0.556813 3:+20830546 MsaT012209.1:CDS
GGCCACAAGGAGCAATTAGT+AGG 0.560286 3:+20830833 MsaT012209.1:intron
GAAGAACCAAGTGTCTGTGC+TGG 0.560864 3:-20830552 None:intergenic
ATGCGCTTGTTATGGTATCT+CGG 0.562285 3:-20829245 None:intergenic
CTTCAACGAAGAGGTGATGC+AGG 0.562980 3:-20828922 None:intergenic
GTTTCAAGCAATGCATCTAA+GGG 0.563003 3:+20830666 MsaT012209.1:CDS
GTGTAGGATTCCTACTATAG+GGG 0.574437 3:+20828886 MsaT012209.1:intron
TTCGTTGAAGTAACATCTCG+TGG 0.581244 3:+20828935 MsaT012209.1:CDS
TCTTCACTAACCAGGTGTTG+AGG 0.585501 3:-20829346 None:intergenic
CTGGGTTCTGAACAGCTGAA+AGG 0.586271 3:+20830630 MsaT012209.1:CDS
GTAGGAAAATTGGGTTTGCG+TGG 0.592801 3:+20830851 MsaT012209.1:CDS
GAGCGGTTGGAGGAGGACAA+AGG 0.594063 3:-20828023 None:intergenic
CACTAACCAGGTGTTGAGGG+AGG 0.596662 3:-20829342 None:intergenic
AATTGTAGATCGTAAATGGA+AGG 0.598698 3:+20829200 MsaT012209.1:CDS
AAGAGGTGATGCAGGTCTAG+TGG 0.600590 3:-20828914 None:intergenic
ACACTTGGTTCTTCAGTGCG+TGG 0.611516 3:+20830561 MsaT012209.1:CDS
TGTAGATCGTAAATGGAAGG+AGG 0.611934 3:+20829203 MsaT012209.1:CDS
AGAGGTTAGCATCACGAACA+AGG 0.617590 3:-20828056 None:intergenic
ACTAACCAGGTGTTGAGGGA+GGG 0.618972 3:-20829341 None:intergenic
TATATAGTTACGGTGGACCT+GGG 0.620088 3:+20830612 MsaT012209.1:CDS
CTTTCAGCTGTTCAGAACCC+AGG 0.626176 3:-20830629 None:intergenic
AGCTTCACAACCAATACGGA+CGG 0.629115 3:+20829465 MsaT012209.1:CDS
TGTAGGATTCCTACTATAGG+GGG 0.629514 3:+20828887 MsaT012209.1:intron
ATTGACATGAAATTTGATGG+CGG 0.676313 3:+20830588 MsaT012209.1:CDS
AACAAGGTCAGAGTAGAGAG+CGG 0.693390 3:-20828040 None:intergenic
GAGATGTTACTTCAACGAAG+AGG 0.709606 3:-20828931 None:intergenic
TGGCGGATATATAGTTACGG+TGG 0.725652 3:+20830605 MsaT012209.1:CDS
TTTGTGAGTTCAGTCTCACG+CGG 0.743764 3:-20828000 None:intergenic
GTTGAAGTAACATCTCGTGG+TGG 0.754811 3:+20828938 MsaT012209.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 20827970 20830883 20827970 ID=MsaG012209
Chr3 mRNA 20827970 20830883 20827970 ID=MsaT012209.1;Parent=MsaG012209
Chr3 exon 20827970 20828127 20827970 ID=MsaT012209.1.exon1;Parent=MsaT012209.1
Chr3 CDS 20827970 20828127 20827970 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Chr3 exon 20828892 20828970 20828892 ID=MsaT012209.1.exon2;Parent=MsaT012209.1
Chr3 CDS 20828892 20828970 20828892 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Chr3 exon 20829198 20829357 20829198 ID=MsaT012209.1.exon3;Parent=MsaT012209.1
Chr3 CDS 20829198 20829357 20829198 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Chr3 exon 20829439 20829516 20829439 ID=MsaT012209.1.exon4;Parent=MsaT012209.1
Chr3 CDS 20829439 20829516 20829439 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Chr3 exon 20830538 20830702 20830538 ID=MsaT012209.1.exon5;Parent=MsaT012209.1
Chr3 CDS 20830538 20830702 20830538 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Chr3 exon 20830834 20830883 20830834 ID=MsaT012209.1.exon6;Parent=MsaT012209.1
Chr3 CDS 20830834 20830883 20830834 ID=cds.MsaT012209.1;Parent=MsaT012209.1
Gene Sequence

>MsaT012209.1

ATGACAAGTAACCAGAATCAAAACCAAAATCCGCGTGAGACTGAACTCACAAACCTTTGTCCTCCTCCAACCGCTCTCTACTCTGACCTTGTTCGTGATGCTAACCTCTTCTGGGAAAAACTTCAATCTTTTCATGATTCGCTTGGTATCAAATTAGAGATTCCTACTATAGGGGGTAAGCCACTAGACCTGCATCACCTCTTCGTTGAAGTAACATCTCGTGGTGGTATTGAAAAGGTAATTGTAGATCGTAAATGGAAGGAGGTAATAATGAGCTTCAACTTCCGAGATACCATAACAAGCGCATCATTCATGGTGCGCAAGACCTATCTATCGTTGCTTTATCACTTTGAACAAGCGTATTATTTTCGCAAGCAAGTCCCTCCCTCAACACCTGATGCTTTGAGTGGAAACGTTGCCAATAGCTTCACAACCAATACGGACGGTGCTGCTATCAATGATTCACCTGTTCAGGTTAGTCCAATTAGTCCAGCACAGACACTTGGTTCTTCAGTGCGTGGAACAATTGACATGAAATTTGATGGCGGATATATAGTTACGGTGGACCTGGGTTCTGAACAGCTGAAAGGTGTCCTGTATCATGTTTCAAGCAATGCATCTAAGGGTTCTTCTATTGAAGGCCACAAGGAGCAATTAGTAGGAAAATTGGGTTTGCGTGGAGGAATCTGA

Protein sequence

>MsaT012209.1

MTSNQNQNQNPRETELTNLCPPPTALYSDLVRDANLFWEKLQSFHDSLGIKLEIPTIGGKPLDLHHLFVEVTSRGGIEKVIVDRKWKEVIMSFNFRDTITSASFMVRKTYLSLLYHFEQAYYFRKQVPPSTPDALSGNVANSFTTNTDGAAINDSPVQVSPISPAQTLGSSVRGTIDMKFDGGYIVTVDLGSEQLKGVLYHVSSNASKGSSIEGHKEQLVGKLGLRGGI*