Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012269 | XP_013459206.1 | 92.937 | 269 | 13 | 1 | 1 | 263 | 1 | 269 | 0.0 | 514 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012269 | sp|Q9M202|ZAT9_ARATH | 27.509 | 269 | 145 | 10 | 8 | 254 | 6 | 246 | 4.95e-14 | 73.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012269 | A0A072V3Z9 | 92.937 | 269 | 13 | 1 | 1 | 263 | 1 | 269 | 0.0 | 514 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG012269 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012269 | MtrunA17_Chr3g0079061 | 85.818 | 275 | 27 | 1 | 1 | 263 | 1 | 275 | 1.99e-174 | 481 |
| MsaG012269 | MtrunA17_Chr3g0079131 | 64.493 | 276 | 84 | 3 | 1 | 263 | 1 | 275 | 2.31e-114 | 329 |
| MsaG012269 | MtrunA17_Chr3g0079091 | 77.401 | 177 | 28 | 1 | 1 | 165 | 1 | 177 | 3.05e-84 | 249 |
| MsaG012269 | MtrunA17_Chr4g0018761 | 30.078 | 256 | 153 | 6 | 3 | 255 | 4 | 236 | 7.12e-25 | 99.4 |
| MsaG012269 | MtrunA17_Chr7g0231181 | 27.036 | 307 | 162 | 10 | 8 | 255 | 6 | 309 | 3.90e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012269 | AT3G60580.1 | 27.509 | 269 | 145 | 10 | 8 | 254 | 6 | 246 | 5.03e-15 | 73.6 |
| MsaG012269 | AT1G02030.1 | 26.172 | 256 | 149 | 10 | 8 | 254 | 7 | 231 | 4.40e-13 | 67.8 |
Find 44 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTGTTGGGTTATTTCT+TGG | 0.241989 | 3:+21743603 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3:+21743694 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3:+21743693 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3:+21743675 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3:-21743099 | MsaT012269.1:CDS |
| TTTCTTGGATTAACAATTGA+TGG | 0.326711 | 3:+21743618 | None:intergenic |
| CTTTGAATCTGGTCAGGTAT+TGG | 0.342520 | 3:-21743088 | MsaT012269.1:CDS |
| ACAATCAGCGTATAGTGATT+TGG | 0.373049 | 3:-21743388 | MsaT012269.1:CDS |
| AAGATTGAGGATTGTTTGTT+GGG | 0.385971 | 3:+21743592 | None:intergenic |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3:+21743682 | None:intergenic |
| AAAGATTGAGGATTGTTTGT+TGG | 0.403304 | 3:+21743591 | None:intergenic |
| TGTCATGAAGTGTTTCAATC+TGG | 0.407883 | 3:-21743240 | MsaT012269.1:CDS |
| TGACATCTTTGAATCTGGTC+AGG | 0.411982 | 3:-21743094 | MsaT012269.1:CDS |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3:-21743791 | MsaT012269.1:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3:+21743695 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3:+21743046 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3:-21743341 | MsaT012269.1:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3:+21743579 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3:-21743555 | MsaT012269.1:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3:+21743653 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3:-21743177 | MsaT012269.1:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3:+21743727 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3:-21743513 | MsaT012269.1:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3:+21743547 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3:+21743471 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3:+21743546 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3:+21743645 | None:intergenic |
| TGAATCTGGTCAGGTATTGG+AGG | 0.506890 | 3:-21743085 | MsaT012269.1:CDS |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3:+21743728 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3:-21743475 | MsaT012269.1:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3:-21743168 | MsaT012269.1:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3:-21743186 | MsaT012269.1:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3:-21743676 | MsaT012269.1:CDS |
| TTGGACATGAAGAGATCCAT+AGG | 0.540507 | 3:-21743206 | MsaT012269.1:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3:+21743696 | None:intergenic |
| GGTATTGGAGGAACACATGA+AGG | 0.581569 | 3:-21743073 | MsaT012269.1:CDS |
| GAATGAATGGAAACAAATTG+AGG | 0.585752 | 3:-21743328 | MsaT012269.1:CDS |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3:-21743762 | MsaT012269.1:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3:-21743363 | MsaT012269.1:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3:-21743551 | MsaT012269.1:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3:+21743260 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3:-21743816 | None:intergenic |
| AGAGAATACACAATTTAACA+TGG | 0.696888 | 3:-21743439 | MsaT012269.1:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3:+21743548 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 21743038 | 21743829 | 21743038 | ID=MsaG012269 |
| Chr3 | mRNA | 21743038 | 21743829 | 21743038 | ID=MsaT012269.1;Parent=MsaG012269 |
| Chr3 | exon | 21743038 | 21743829 | 21743038 | ID=MsaT012269.1.exon1;Parent=MsaT012269.1 |
| Chr3 | CDS | 21743038 | 21743829 | 21743038 | ID=cds.MsaT012269.1;Parent=MsaT012269.1 |
| Gene Sequence |
| Protein sequence |
