Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012330 | XP_013459206.1 | 91.078 | 269 | 18 | 1 | 1 | 263 | 1 | 269 | 6.68e-180 | 505 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012330 | sp|Q9M202|ZAT9_ARATH | 26.766 | 269 | 147 | 10 | 8 | 254 | 6 | 246 | 1.59e-12 | 69.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012330 | A0A072V3Z9 | 91.078 | 269 | 18 | 1 | 1 | 263 | 1 | 269 | 3.19e-180 | 505 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG012330 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG003754 | MsaG012330 | 0.809867 | 2.614656e-50 | 4.450761e-48 |
| MsaG012214 | MsaG012330 | 0.806799 | 1.171148e-49 | 1.852088e-47 |
| MsaG045313 | MsaG012330 | 0.814000 | 3.322277e-51 | 6.262665e-49 |
| MsaG046573 | MsaG012330 | 0.813291 | 4.750419e-51 | 8.796798e-49 |
| MsaG012092 | MsaG012330 | 0.827641 | 2.498193e-54 | 6.763099e-52 |
| MsaG020183 | MsaG012330 | 0.816317 | 1.021370e-51 | 2.041504e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012330 | MtrunA17_Chr3g0079061 | 84.000 | 275 | 32 | 1 | 1 | 263 | 1 | 275 | 8.03e-170 | 469 |
| MsaG012330 | MtrunA17_Chr3g0079131 | 64.493 | 276 | 84 | 3 | 1 | 263 | 1 | 275 | 6.25e-114 | 328 |
| MsaG012330 | MtrunA17_Chr3g0079091 | 75.706 | 177 | 31 | 1 | 1 | 165 | 1 | 177 | 1.08e-81 | 243 |
| MsaG012330 | MtrunA17_Chr4g0018761 | 29.297 | 256 | 155 | 5 | 3 | 255 | 4 | 236 | 5.39e-24 | 97.1 |
| MsaG012330 | MtrunA17_Chr7g0231181 | 26.797 | 306 | 164 | 10 | 8 | 255 | 6 | 309 | 6.78e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012330 | AT3G60580.1 | 26.766 | 269 | 147 | 10 | 8 | 254 | 6 | 246 | 1.61e-13 | 69.3 |
| MsaG012330 | AT1G02030.1 | 26.482 | 253 | 152 | 8 | 8 | 254 | 7 | 231 | 1.61e-12 | 66.2 |
Find 43 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTGGTGGGTTACTTCT+TGG | 0.249003 | 3:-22688602 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3:-22688511 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3:-22688512 | None:intergenic |
| CTTCTTGGATTAACAATTGA+TGG | 0.303146 | 3:-22688587 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3:-22688530 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3:+22689106 | MsaT012330.1:CDS |
| TTCAAAGATTGAGGATTGTT+TGG | 0.332480 | 3:-22688617 | None:intergenic |
| CTTTGAATCTGGTCACGTAT+TGG | 0.390852 | 3:+22689117 | MsaT012330.1:CDS |
| ACAATCAGCGTATAGCGATT+TGG | 0.398813 | 3:+22688817 | MsaT012330.1:CDS |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3:-22688523 | None:intergenic |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3:+22688414 | MsaT012330.1:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3:-22688510 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3:-22689159 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3:+22688864 | MsaT012330.1:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3:-22688626 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3:+22688650 | MsaT012330.1:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3:-22688552 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3:+22689028 | MsaT012330.1:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3:-22688478 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3:+22688692 | MsaT012330.1:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3:-22688658 | None:intergenic |
| AAGATTGAGGATTGTTTGGT+GGG | 0.486813 | 3:-22688613 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3:-22688734 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3:-22688659 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3:-22688560 | None:intergenic |
| TGAATCTGGTCACGTATTGG+AGG | 0.509112 | 3:+22689120 | MsaT012330.1:CDS |
| AAAGATTGAGGATTGTTTGG+TGG | 0.514150 | 3:-22688614 | None:intergenic |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3:-22688477 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3:+22688730 | MsaT012330.1:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3:+22689037 | MsaT012330.1:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3:+22689019 | MsaT012330.1:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3:+22688529 | MsaT012330.1:CDS |
| CGTATTGGAGGAACACATGA+AGG | 0.564625 | 3:+22689132 | MsaT012330.1:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3:-22688509 | None:intergenic |
| GAATGAATGGAAACAAATTG+AGG | 0.585752 | 3:+22688877 | MsaT012330.1:CDS |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3:+22688443 | MsaT012330.1:CDS |
| TCGGACATGAAGATATCCAT+AGG | 0.592226 | 3:+22688999 | MsaT012330.1:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3:+22688842 | MsaT012330.1:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3:+22688654 | MsaT012330.1:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3:-22688945 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3:+22688389 | None:intergenic |
| AGAGAATATAGAATTTAACA+TGG | 0.705093 | 3:+22688766 | MsaT012330.1:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3:-22688657 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 22688398 | 22689189 | 22688398 | ID=MsaG012330 |
| Chr3 | mRNA | 22688398 | 22689189 | 22688398 | ID=MsaT012330.1;Parent=MsaG012330 |
| Chr3 | exon | 22688398 | 22689189 | 22688398 | ID=MsaT012330.1.exon1;Parent=MsaT012330.1 |
| Chr3 | CDS | 22688398 | 22689189 | 22688398 | ID=cds.MsaT012330.1;Parent=MsaT012330.1 |
| Gene Sequence |
| Protein sequence |
