AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012332


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012332 MtrunA17_Chr3g0079131 91.273 275 20 1 1 271 1 275 2.38e-178 491
MsaG012332 MtrunA17_Chr3g0079061 63.273 275 97 2 1 271 1 275 5.16e-115 331
MsaG012332 MtrunA17_Chr3g0079091 63.842 177 60 2 1 173 1 177 2.97e-64 199
MsaG012332 MtrunA17_Chr4g0018761 28.731 268 153 7 3 265 4 238 1.98e-24 98.6
MsaG012332 MtrunA17_Chr7g0231181 25.538 325 172 11 4 269 2 315 6.36e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012332 AT1G02030.1 25.830 271 149 8 5 264 4 233 5.33e-12 64.7
MsaG012332 AT3G60580.1 26.354 277 148 10 7 262 5 246 1.36e-11 63.9

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTCGTAGACAATTTAATT+TGG 0.272723 3:+22709814 MsaT012332.1:CDS
AACAATCTATCTGAAGAAAT+TGG 0.300908 3:+22710139 MsaT012332.1:CDS
TGAGAATACACAAGTTAAAT+TGG 0.302912 3:+22709913 MsaT012332.1:CDS
ATACTATAGTGAGAAGAAAA+AGG 0.312924 3:+22710015 MsaT012332.1:CDS
TAAATTGGTGTATAGTGATT+TGG 0.323391 3:+22709928 MsaT012332.1:CDS
CTATTATTTGTGTGAATGAA+TGG 0.399412 3:+22709975 MsaT012332.1:CDS
TCACGTGCTTGGTTGTTGAT+TGG 0.399497 3:-22709599 None:intergenic
TATCATACCCTTGTAAACTT+TGG 0.406116 3:+22709734 MsaT012332.1:CDS
TCTGAAGAAATTGGTATAAG+TGG 0.426032 3:+22710148 MsaT012332.1:CDS
AATAGAGAGAGTTTGATGTT+TGG 0.448411 3:-22709639 None:intergenic
TACAGAAGCTGCTGAAACAT+TGG 0.473334 3:+22709952 MsaT012332.1:CDS
TATTCTTACCAAAGTTTACA+AGG 0.477139 3:-22709742 None:intergenic
TCACAAGTGCACATATTGCT+TGG 0.480968 3:+22710201 MsaT012332.1:CDS
CAATGCTTTCCCATTAGAAA+AGG 0.484168 3:-22709525 None:intergenic
AAGATCGAAGATTATGCATA+GGG 0.485747 3:-22709697 None:intergenic
TGTACTTGAGGAACACAAAA+CGG 0.523616 3:+22710243 MsaT012332.1:CDS
CAACTGAGTGTTCACGTGCT+TGG 0.533072 3:-22709610 None:intergenic
TGAATCTGATCATGTACTTG+AGG 0.537940 3:+22710231 MsaT012332.1:CDS
ATAATCTTCGATCTTTGAAG+AGG 0.557615 3:+22709705 MsaT012332.1:CDS
AAAGATCGAAGATTATGCAT+AGG 0.557994 3:-22709698 None:intergenic
AGAAATTGGTATAAGTGGAA+AGG 0.574832 3:+22710153 MsaT012332.1:CDS
AGAGAATACAATATTTAATG+TGG 0.616169 3:+22709841 MsaT012332.1:CDS
TGTGGCTGAAGAAAATGAAG+AGG 0.616323 3:+22709859 MsaT012332.1:CDS
ATTCTTACCAAAGTTTACAA+GGG 0.625545 3:-22709741 None:intergenic
TCTAATGGGAAAGCATTGGG+AGG 0.669450 3:+22709530 MsaT012332.1:CDS
GAATGAATGGAAACAAGTTG+AGG 0.698163 3:+22709988 MsaT012332.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 22709485 22710300 22709485 ID=MsaG012332
Chr3 mRNA 22709485 22710300 22709485 ID=MsaT012332.1;Parent=MsaG012332
Chr3 exon 22709485 22710300 22709485 ID=MsaT012332.1.exon1;Parent=MsaT012332.1
Chr3 CDS 22709485 22710300 22709485 ID=cds.MsaT012332.1;Parent=MsaT012332.1
Gene Sequence

>MsaT012332.1

ATGGAGAACAATAGAAGAGAATGTCACATTTGTAACAAGTCCTTTTCTAATGGGAAAGCATTGGGAGGCCACATGAAAACTCACTTAGCTAAACTACTAATCCCTCTAAAATCTCCAATCAACAACCAAGCACGTGAACACTCAGTTGAGTCAACCAAACATCAAACTCTCTCTATTTCTACATCTTCCTCATCAATTTCCAATCCAAAAAACCCTATGCATAATCTTCGATCTTTGAAGAGGAATTTTTATCATACCCTTGTAAACTTTGGTAAGAATAGTGTGTTTGATTCTTTTCCAAAAAACCCAACTGGAAAAAGATCGAAACGCGGTCGTAGACAATTTAATTTGGCTGAAGAGAATACAATATTTAATGTGGCTGAAGAAAATGAAGAGGATACACAATTTAATGCAGTTGAAGAAATAAATGAGAATACACAAGTTAAATTGGTGTATAGTGATTTGGATACAGAAGCTGCTGAAACATTGGCTATTATTTGTGTGAATGAATGGAAACAAGTTGAGGAGAAATACTATAGTGAGAAGAAAAAGGTTAGTGAAAATGGCAACATCATGTTTGAATGCGATATTTGTCATGAAGTGTTTCAATATTGCAAAGATCTTGTTAGACATGAAGCAATCCATAAGAAAAATAACAATCTATCTGAAGAAATTGGTATAAGTGGAAAGGAATATGATGTTGTAAATGAAGAAGTTCACAAGTGCACATATTGCTTGGAATTTTTTGAATCTGATCATGTACTTGAGGAACACAAAACGGTACACTTATCCAATTTTTCTGATTCTAACCCATAG

Protein sequence

>MsaT012332.1

MENNRRECHICNKSFSNGKALGGHMKTHLAKLLIPLKSPINNQAREHSVESTKHQTLSISTSSSSISNPKNPMHNLRSLKRNFYHTLVNFGKNSVFDSFPKNPTGKRSKRGRRQFNLAEENTIFNVAEENEEDTQFNAVEEINENTQVKLVYSDLDTEAAETLAIICVNEWKQVEEKYYSEKKKVSENGNIMFECDICHEVFQYCKDLVRHEAIHKKNNNLSEEIGISGKEYDVVNEEVHKCTYCLEFFESDHVLEEHKTVHLSNFSDSNP*