Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012335 | XP_013459208.1 | 89.818 | 275 | 23 | 2 | 1 | 270 | 1 | 275 | 5.73e-171 | 483 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012335 | sp|Q39092|ZAT1_ARATH | 25.926 | 270 | 149 | 9 | 5 | 263 | 4 | 233 | 7.18e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012335 | A0A072UTD1 | 89.818 | 275 | 23 | 2 | 1 | 270 | 1 | 275 | 2.74e-171 | 483 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG012335 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000273 | MsaG012335 | 0.830454 | 5.238145e-55 | 1.535648e-52 |
| MsaG000457 | MsaG012335 | 0.849802 | 4.866705e-60 | 2.619787e-57 |
| MsaG004802 | MsaG012335 | 0.881819 | 3.714985e-70 | 7.302768e-67 |
| MsaG006203 | MsaG012335 | 0.812487 | 7.111073e-51 | 1.290758e-48 |
| MsaG006269 | MsaG012335 | 0.807675 | 7.652963e-50 | 1.235709e-47 |
| MsaG007848 | MsaG012335 | 0.822669 | 3.691430e-53 | 8.716081e-51 |
| MsaG007852 | MsaG012335 | 0.825439 | 8.317054e-54 | 2.117575e-51 |
| MsaG010547 | MsaG012335 | 0.826989 | 3.572782e-54 | 9.496923e-52 |
| MsaG012335 | MsaG015023 | 0.817665 | 5.104111e-52 | 1.056139e-49 |
| MsaG012335 | MsaG016670 | 0.832816 | 1.379679e-55 | 4.331655e-53 |
| MsaG012335 | MsaG019271 | 0.860641 | 3.528345e-63 | 2.811546e-60 |
| MsaG012335 | MsaG019411 | 0.811008 | 1.486931e-50 | 2.602420e-48 |
| MsaG012335 | MsaG022563 | 0.855193 | 1.437903e-61 | 9.354012e-59 |
| MsaG012335 | MsaG023954 | 0.833412 | 9.817598e-56 | 3.137026e-53 |
| MsaG012335 | MsaG025955 | 0.822662 | 3.704022e-53 | 8.744153e-51 |
| MsaG012335 | MsaG027152 | 0.831573 | 2.791244e-55 | 8.452279e-53 |
| MsaG012335 | MsaG028241 | 0.825768 | 6.958633e-54 | 1.787959e-51 |
| MsaG012335 | MsaG029279 | 0.803139 | 6.764344e-49 | 9.822312e-47 |
| MsaG012335 | MsaG032291 | 0.851465 | 1.666526e-60 | 9.500811e-58 |
| MsaG012335 | MsaG033280 | 0.811435 | 1.202767e-50 | 2.127222e-48 |
| MsaG012335 | MsaG038035 | 0.801151 | 1.726542e-48 | 2.395790e-46 |
| MsaG012335 | MsaG044373 | 0.822505 | 4.027511e-53 | 9.467555e-51 |
| MsaG012335 | MsaG045188 | 0.802147 | 1.080830e-48 | 1.534117e-46 |
| MsaG012335 | MsaG046858 | 0.818724 | 2.947396e-52 | 6.268182e-50 |
| MsaG012335 | MsaG005679 | 0.809425 | 3.250461e-50 | 5.474450e-48 |
| MsaG012335 | MsaG003075 | 0.820447 | 1.196785e-52 | 2.663577e-50 |
| MsaG012335 | MsaG019850 | 0.802233 | 1.038078e-48 | 1.476333e-46 |
| MsaG012335 | MsaG026083 | 0.815443 | 1.596555e-51 | 3.121084e-49 |
| MsaG012335 | MsaG032517 | 0.805391 | 2.309277e-49 | 3.532894e-47 |
| MsaG012335 | MsaG043094 | 0.905303 | 1.168395e-79 | 8.195428e-76 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012335 | MtrunA17_Chr3g0079131 | 89.818 | 275 | 23 | 2 | 1 | 270 | 1 | 275 | 5.27e-175 | 483 |
| MsaG012335 | MtrunA17_Chr3g0079061 | 61.818 | 275 | 100 | 2 | 1 | 270 | 1 | 275 | 2.39e-112 | 325 |
| MsaG012335 | MtrunA17_Chr3g0079091 | 63.277 | 177 | 60 | 2 | 1 | 172 | 1 | 177 | 2.19e-64 | 199 |
| MsaG012335 | MtrunA17_Chr4g0018761 | 28.358 | 268 | 153 | 7 | 3 | 264 | 4 | 238 | 6.69e-24 | 97.1 |
| MsaG012335 | MtrunA17_Chr7g0231181 | 24.383 | 324 | 176 | 9 | 4 | 268 | 2 | 315 | 6.85e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012335 | AT1G02030.1 | 25.926 | 270 | 149 | 9 | 5 | 263 | 4 | 233 | 7.30e-12 | 64.3 |
| MsaG012335 | AT3G60580.1 | 26.449 | 276 | 148 | 10 | 7 | 261 | 5 | 246 | 2.71e-11 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGATTATGCATAGGTTT+TGG | 0.234409 | 3:-22723950 | None:intergenic |
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3:+22724072 | MsaT012335.1:CDS |
| AACAATATATCTGAAGAAAT+TGG | 0.302487 | 3:+22724397 | MsaT012335.1:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3:+22724171 | MsaT012335.1:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3:+22724273 | MsaT012335.1:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3:+22724186 | MsaT012335.1:CDS |
| CAACTGAGTGTTCAGGTGTT+TGG | 0.326373 | 3:-22723871 | None:intergenic |
| TCAGGTGTTTGGTTGTTGAT+TGG | 0.360834 | 3:-22723860 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.418644 | 3:+22724406 | MsaT012335.1:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3:+22724233 | MsaT012335.1:CDS |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3:-22723878 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3:-22723900 | None:intergenic |
| TATTCTTACTAAAGTTTACA+AGG | 0.462564 | 3:-22724000 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3:+22724459 | MsaT012335.1:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3:-22723786 | None:intergenic |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3:+22724210 | MsaT012335.1:CDS |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3:+22724501 | MsaT012335.1:CDS |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3:+22724489 | MsaT012335.1:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3:+22723963 | MsaT012335.1:CDS |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3:+22724411 | MsaT012335.1:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.576327 | 3:-22723956 | None:intergenic |
| TGTAGCTGAAGAAAATGAAG+AGG | 0.590626 | 3:+22724117 | MsaT012335.1:CDS |
| ATTCTTACTAAAGTTTACAA+GGG | 0.609953 | 3:-22723999 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3:+22723791 | MsaT012335.1:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3:+22724246 | MsaT012335.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 22723746 | 22724558 | 22723746 | ID=MsaG012335 |
| Chr3 | mRNA | 22723746 | 22724558 | 22723746 | ID=MsaT012335.1;Parent=MsaG012335 |
| Chr3 | exon | 22723746 | 22724558 | 22723746 | ID=MsaT012335.1.exon1;Parent=MsaT012335.1 |
| Chr3 | CDS | 22723746 | 22724558 | 22723746 | ID=cds.MsaT012335.1;Parent=MsaT012335.1 |
| Gene Sequence |
| Protein sequence |
