AlfalfaGEDB | MsaG012428

M. sativa ssp. caerulea / MsaG012428

KEGG: K00670 K20791
GO: -
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012428	KEH33239.1	86.093	151	18	1	1	151	8	155	2.55e-86	259

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012428	sp\|Q9FKI4\|NAA10_ARATH	45.455	154	73	4	6	148	2	155	3.27e-29	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012428	A0A072UTR7	86.093	151	18	1	1	151	8	155	1.22e-86	259

TFs/TRs:

Gene ID	Type	Classification
MsaG012428	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000004	MsaG012428	0.830600	4.823951e-55	1.420176e-52
MsaG000170	MsaG012428	0.863816	3.780830e-64	3.407475e-61
MsaG000532	MsaG012428	0.833867	7.567105e-56	2.450299e-53
MsaG001134	MsaG012428	0.810100	2.331262e-50	3.990795e-48
MsaG001542	MsaG012428	0.853381	4.769844e-61	2.908737e-58
MsaG002099	MsaG012428	0.845238	8.630827e-59	3.985619e-56
MsaG003514	MsaG012428	0.851619	1.508489e-60	8.645220e-58
MsaG004085	MsaG012428	0.801863	1.235816e-48	1.742841e-46
MsaG004621	MsaG012428	0.813855	3.573502e-51	6.712190e-49
MsaG005202	MsaG012428	0.812916	5.734668e-51	1.052083e-48
MsaG005410	MsaG012428	0.816246	1.059510e-51	2.113864e-49
MsaG005680	MsaG012428	0.825857	6.628292e-54	1.707357e-51
MsaG005764	MsaG012428	0.803160	6.695050e-49	9.726602e-47
MsaG005792	MsaG012428	0.859869	6.025769e-63	4.661472e-60
MsaG005809	MsaG012428	0.823688	2.139891e-53	5.194207e-51
MsaG006255	MsaG012428	0.838402	5.403164e-57	2.006385e-54
MsaG006601	MsaG012428	0.825365	8.658150e-54	2.199961e-51
MsaG006777	MsaG012428	0.802854	7.740975e-49	1.116667e-46
MsaG007139	MsaG012428	0.861857	1.509485e-63	1.259809e-60
MsaG007356	MsaG012428	0.834429	5.478892e-56	1.804246e-53
MsaG007388	MsaG012428	0.808823	4.367471e-50	7.249829e-48
MsaG007442	MsaG012428	0.811915	9.465059e-51	1.693950e-48
MsaG007455	MsaG012428	0.845363	7.985362e-59	3.703117e-56
MsaG008029	MsaG012428	0.832255	1.897402e-55	5.860747e-53
MsaG008481	MsaG012428	0.801432	1.513281e-48	2.113314e-46
MsaG008433	MsaG012428	0.841638	7.813179e-58	3.211700e-55
MsaG009301	MsaG012428	0.809043	3.921350e-50	6.543798e-48
MsaG009346	MsaG012428	0.809115	3.786057e-50	6.328944e-48
MsaG009725	MsaG012428	0.811432	1.204065e-50	2.129425e-48
MsaG009726	MsaG012428	0.823023	3.055926e-53	7.284931e-51
MsaG010266	MsaG012428	0.835595	2.793788e-56	9.526410e-54
MsaG010829	MsaG012428	0.834065	6.755919e-56	2.200474e-53
MsaG011572	MsaG012428	0.801082	1.783270e-48	2.470628e-46
MsaG011584	MsaG012428	0.817795	4.772934e-52	9.909547e-50
MsaG011699	MsaG012428	0.807213	9.582013e-50	1.530168e-47
MsaG012428	MsaG012522	0.809287	3.478571e-50	5.838766e-48
MsaG012428	MsaG014075	0.806490	1.359649e-49	2.134666e-47
MsaG012428	MsaG016270	0.818912	2.672931e-52	5.712591e-50
MsaG012428	MsaG017050	0.806513	1.345067e-49	2.112894e-47
MsaG012428	MsaG017128	0.801698	1.335674e-48	1.876554e-46
MsaG012428	MsaG017258	0.828665	1.418920e-54	3.953238e-52
MsaG012428	MsaG018065	0.836438	1.711035e-56	5.984411e-54
MsaG012428	MsaG018498	0.832968	1.265256e-55	3.990234e-53
MsaG012428	MsaG018666	0.804551	3.453397e-49	5.181309e-47
MsaG012428	MsaG018822	0.833089	1.180833e-55	3.737407e-53
MsaG012428	MsaG018776	0.801087	1.779006e-48	2.465016e-46
MsaG012428	MsaG018985	0.830591	4.848596e-55	1.427057e-52
MsaG012428	MsaG019577	0.813588	4.089934e-51	7.630721e-49
MsaG012428	MsaG019969	0.819019	2.527763e-52	5.417596e-50
MsaG012428	MsaG023729	0.839324	3.128349e-57	1.195510e-54
MsaG012428	MsaG023924	0.852083	1.115926e-60	6.501564e-58
MsaG012428	MsaG025423	0.845391	7.847120e-59	3.642491e-56
MsaG012428	MsaG027457	0.833824	7.754520e-56	2.507842e-53
MsaG012428	MsaG027511	0.811496	1.166608e-50	2.066413e-48
MsaG012428	MsaG027542	0.809481	3.162745e-50	5.334046e-48
MsaG012428	MsaG027872	0.814368	2.757958e-51	5.247231e-49
MsaG012428	MsaG028335	0.805125	2.623304e-49	3.988739e-47
MsaG012428	MsaG028700	0.802808	7.911992e-49	1.140131e-46
MsaG012428	MsaG029086	0.838266	5.853126e-57	2.164407e-54
MsaG012428	MsaG029324	0.807068	1.027726e-49	1.635624e-47
MsaG012428	MsaG029711	0.842009	6.239339e-58	2.595490e-55
MsaG012428	MsaG031799	0.835787	2.499164e-56	8.570865e-54
MsaG012428	MsaG032947	0.854956	1.684135e-61	1.086253e-58
MsaG012428	MsaG033728	0.803834	4.862396e-49	7.174874e-47
MsaG012428	MsaG034319	0.823242	2.718113e-53	6.518106e-51
MsaG012428	MsaG034741	0.868681	1.102402e-65	1.209285e-62
MsaG012428	MsaG034836	0.800824	2.012281e-48	2.771794e-46
MsaG012428	MsaG035233	0.803459	5.809570e-49	8.498515e-47
MsaG012428	MsaG035532	0.865629	1.029567e-64	9.973223e-62
MsaG012428	MsaG035533	0.836446	1.703403e-56	5.958964e-54
MsaG012428	MsaG035552	0.873629	2.611453e-67	3.536079e-64
MsaG012428	MsaG036065	0.803948	4.605756e-49	6.814328e-47
MsaG012428	MsaG036109	0.871811	1.051752e-66	1.316479e-63
MsaG012428	MsaG039197	0.801459	1.494339e-48	2.088124e-46
MsaG012428	MsaG040013	0.835695	2.636886e-56	9.017798e-54
MsaG012428	MsaG040428	0.809710	2.825190e-50	4.790931e-48
MsaG012428	MsaG041334	0.824889	1.120363e-53	2.809801e-51
MsaG012428	MsaG041905	0.807480	8.416358e-50	1.352623e-47
MsaG012428	MsaG042380	0.842556	4.478565e-58	1.895946e-55
MsaG012428	MsaG042802	0.818070	4.139160e-52	8.654919e-50
MsaG012428	MsaG043808	0.809958	2.500266e-50	4.265356e-48
MsaG012428	MsaG044187	0.815289	1.727410e-51	3.363713e-49
MsaG012428	MsaG044928	0.867011	3.769836e-65	3.862738e-62
MsaG012428	MsaG044991	0.805840	1.860982e-49	2.877122e-47
MsaG012428	MsaG045832	0.850354	3.415356e-60	1.873537e-57
MsaG012428	MsaG046999	0.863431	4.970213e-64	4.412317e-61
MsaG012428	MsaG001694	0.817006	7.170975e-52	1.458922e-49
MsaG012428	MsaG001554	0.834220	6.178547e-56	2.021837e-53
MsaG012428	MsaG003540	0.808353	5.497243e-50	9.022179e-48
MsaG012428	MsaG009686	0.821377	7.328808e-53	1.671685e-50
MsaG012428	MsaG010137	0.849223	7.044470e-60	3.718371e-57
MsaG012428	MsaG014403	0.805870	1.834036e-49	2.837469e-47
MsaG012428	MsaG013590	0.823573	2.276015e-53	5.507162e-51
MsaG012428	MsaG031891	0.829691	8.023131e-55	2.301472e-52
MsaG012428	MsaG030863	0.853622	4.072525e-61	2.504627e-58
MsaG012428	MsaG031311	0.804880	2.950591e-49	4.460870e-47
MsaG012428	MsaG032474	0.809482	3.161255e-50	5.331665e-48
MsaG012428	MsaG038131	0.812336	7.668478e-51	1.386700e-48
MsaG012428	MsaG036205	0.808696	4.648207e-50	7.692477e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012428	MtrunA17_Chr3g0088851	85.567	97	14	0	1	97	1	97	3.17e-56	172
MsaG012428	MtrunA17_Chr3g0090311	48.387	155	69	4	6	149	2	156	2.34e-34	118
MsaG012428	MtrunA17_Chr3g0098461	38.710	155	86	5	1	149	1	152	8.57e-21	83.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012428	AT5G13780.1	45.455	154	73	4	6	148	2	155	3.32e-30	108

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACACAATGTGCCAATATT+CGG	0.301530	3:-24216606	None:intergenic
CACACAATGTGCCAATATTC+GGG	0.341666	3:-24216605	None:intergenic
GAGCTGCAGAGTTGCTCTTC+CGG	0.345662	3:-24217448	None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG	0.363394	3:+24217469	MsaT012428.1:CDS
CTTGTTACTCGCCCGAATAT+TGG	0.371742	3:+24216594	MsaT012428.1:CDS
CTCCTCATCATCAAAATACT+TGG	0.430558	3:-24217516	None:intergenic
ATTGTGTGAAAAGCTTATTC+AGG	0.437024	3:+24216620	MsaT012428.1:CDS
TGTATAAGAGTCGCAACAAT+TGG	0.448437	3:+24216540	MsaT012428.1:CDS
TGCCGAAAACACCATGGAAC+AGG	0.451481	3:+24216785	MsaT012428.1:CDS
GTTGCTTACCTCCTCTCTAA+AGG	0.454440	3:+24216669	MsaT012428.1:CDS
CTTGGTTTGTTTGAAAAGAT+GGG	0.459057	3:+24217470	MsaT012428.1:CDS
AAGAGCAACTCTGCAGCTCT+TGG	0.462439	3:+24217452	MsaT012428.1:CDS
CAATTGGAGACGTATTAGAT+TGG	0.472024	3:+24216556	MsaT012428.1:CDS
CGTAAACGTGGTATCGCTAC+TGG	0.485343	3:+24216750	MsaT012428.1:CDS
AAGACGTCTCAGTGCACGTC+CGG	0.493597	3:+24217429	MsaT012428.1:CDS
GTCTTGAGGAAGATACTCAA+TGG	0.508440	3:+24217548	MsaT012428.1:CDS
CTTCCTCTTCACCTTTAGAG+AGG	0.526944	3:-24216680	None:intergenic
AGTAACAATGTCGTCAAAGA+AGG	0.537395	3:+24216515	None:intergenic
CCTCTTCACCTTTAGAGAGG+AGG	0.539853	3:-24216677	None:intergenic
CCTCCTCTCTAAAGGTGAAG+AGG	0.541904	3:+24216677	MsaT012428.1:CDS
TCTTGAGGAAGATACTCAAT+GGG	0.545420	3:+24217549	MsaT012428.1:CDS
CTCTCTAAAGGTGAAGAGGA+AGG	0.549289	3:+24216681	MsaT012428.1:CDS
ATAGGTATGGTGAACAAAGA+GGG	0.575631	3:-24216727	None:intergenic
AACAAACCTAACCTGTTCCA+TGG	0.593695	3:-24216796	None:intergenic
TGAAAAGCTTATTCAGGTCG+CGG	0.594058	3:+24216626	MsaT012428.1:CDS
TCTCTAAAGGTGAAGAGGAA+GGG	0.603620	3:+24216682	MsaT012428.1:CDS
GATAGGTATGGTGAACAAAG+AGG	0.607709	3:-24216728	None:intergenic
AGGAGGACGCTATTGTCTTG+AGG	0.615172	3:+24217534	MsaT012428.1:CDS
AAAGGTGAAGAGGAAGGGAA+TGG	0.618992	3:+24216687	MsaT012428.1:CDS
TTACACACTTCATGAGGTGA+AGG	0.620811	3:+24217493	MsaT012428.1:CDS
CACCATACCTATCGTAAACG+TGG	0.628725	3:+24216738	MsaT012428.1:CDS
GAGTGGGTCTCACAGTTAGG+TGG	0.634645	3:-24217582	None:intergenic
AACCGCTGCCGAAAACACCA+TGG	0.639355	3:+24216779	MsaT012428.1:CDS
TACCACGTTTACGATAGGTA+TGG	0.651983	3:-24216740	None:intergenic
CAGGTCGCGGACGATTGCAG+CGG	0.655833	3:+24216639	MsaT012428.1:CDS
GATGGGTTACACACTTCATG+AGG	0.658895	3:+24217487	MsaT012428.1:CDS
AGCGATACCACGTTTACGAT+AGG	0.681782	3:-24216745	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	24216522	24217589	24216522	ID=MsaG012428
Chr3	mRNA	24216522	24217589	24216522	ID=MsaT012428.1;Parent=MsaG012428
Chr3	exon	24216522	24216806	24216522	ID=MsaT012428.1.exon1;Parent=MsaT012428.1
Chr3	CDS	24216522	24216806	24216522	ID=cds.MsaT012428.1;Parent=MsaT012428.1
Chr3	exon	24217419	24217589	24217419	ID=MsaT012428.1.exon2;Parent=MsaT012428.1
Chr3	CDS	24217419	24217589	24217419	ID=cds.MsaT012428.1;Parent=MsaT012428.1

Gene Sequence

>MsaT012428.1

ATGTCGTCAAAGAAGGTGTGTATAAGAGTCGCAACAATTGGAGACGTATTAGATTGGAACAGTAACAACAATCTTGTTACTCGCCCGAATATTGGCACATTGTGTGAAAAGCTTATTCAGGTCGCGGACGATTGCAGCGGCTGTATTGTTGCTTACCTCCTCTCTAAAGGTGAAGAGGAAGGGAATGGTCACTTTAGTATCGCTTCCCTCTTTGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGGTCTTATAACCGCTGCCGAAAACACCATGGAACAGGTGGGTGCTAAAGACGTCTCAGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCATGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGAGGAAGATACTCAATGGGAAGCATGTTGCCACCTAA

Protein sequence

>MsaT012428.1

MSSKKVCIRVATIGDVLDWNSNNNLVTRPNIGTLCEKLIQVADDCSGCIVAYLLSKGEEEGNGHFSIASLFVHHTYRKRGIATGLITAAENTMEQVGAKDVSVHVRKSNSAALGLFEKMGYTLHEVKAKYFDDEEDAIVLRKILNGKHVAT*