AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012458


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012458 MtrunA17_Chr2g0329151 48.889 135 59 2 11 137 18 150 4.12e-38 135
MsaG012458 MtrunA17_Chr5g0440591 51.754 114 49 1 29 136 45 158 1.81e-36 131
MsaG012458 MtrunA17_Chr3g0118361 52.252 111 47 1 32 136 51 161 4.18e-36 130
MsaG012458 MtrunA17_Chr3g0124291 46.154 104 53 1 33 133 32 135 9.95e-31 114
MsaG012458 MtrunA17_Chr3g0080401 87.500 56 7 0 81 136 1 56 1.87e-30 112
MsaG012458 MtrunA17_Chr8g0382561 48.649 111 52 2 26 133 17 125 1.02e-27 106
MsaG012458 MtrunA17_Chr6g0473191 75.000 56 14 0 81 136 1 56 1.70e-25 98.2
MsaG012458 MtrunA17_Chr6g0476301 73.214 56 15 0 81 136 1 56 8.83e-25 97.4
MsaG012458 MtrunA17_Chr6g0476291 69.643 56 17 0 81 136 1 56 1.34e-23 92.4
MsaG012458 MtrunA17_Chr7g0260281 45.455 66 36 0 70 135 1 66 1.34e-16 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012458 AT2G27050.1 51.163 129 51 2 20 136 31 159 1.55e-38 137
MsaG012458 AT1G73730.2 50.769 130 54 2 16 137 11 138 2.18e-38 136
MsaG012458 AT1G73730.1 50.769 130 54 2 16 137 23 150 2.70e-38 136
MsaG012458 AT3G20770.1 48.551 138 63 2 5 136 23 158 1.27e-37 135
MsaG012458 AT5G10120.1 43.243 111 57 2 29 133 19 129 9.48e-29 109
MsaG012458 AT5G21120.2 45.217 115 50 2 38 141 60 172 4.55e-25 99.8
MsaG012458 AT5G21120.3 45.217 115 50 2 38 141 53 165 4.63e-25 99.8
MsaG012458 AT5G21120.1 45.217 115 50 2 38 141 53 165 4.63e-25 99.8
MsaG012458 AT5G65100.1 34.921 126 61 1 29 133 34 159 2.80e-24 97.4

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACATTCTTCTTCATATT+TGG 0.124414 3:-25043494 None:intergenic
GATGTTCGTGGCTTCGTCTA+TGG 0.352458 3:+25043367 MsaT012458.1:CDS
CAAACCAATGATTGGCTCTT+CGG 0.371062 3:+25043408 MsaT012458.1:CDS
ATCATTGGTTTGCCATTGTC+AGG 0.373218 3:-25043397 None:intergenic
TCATATTTGGCGATTGCAGC+TGG 0.389765 3:-25043481 None:intergenic
GTGCACAAGAGGGCATATTA+AGG 0.410074 3:+25042259 MsaT012458.1:CDS
CGGAAAATCTACGCGGTTGG+TGG 0.429144 3:+25043428 MsaT012458.1:CDS
AGGAACTAGAGACAAAGATA+TGG 0.443648 3:+25042142 MsaT012458.1:CDS
GACAATGGCTCGTGCACAAG+AGG 0.447975 3:+25042248 MsaT012458.1:CDS
GACAATGGCAAACCAATGAT+TGG 0.467907 3:+25043400 MsaT012458.1:CDS
GAAGCACCGTTATCAACGTT+GGG 0.485553 3:-25042051 None:intergenic
AAAGTAAACTTTGAGGAAAA+CGG 0.500540 3:+25043457 MsaT012458.1:CDS
AACAATGCAAAACAAGATAA+CGG 0.516750 3:+25042075 MsaT012458.1:CDS
AAATCTACGCGGTTGGTGGA+AGG 0.522592 3:+25043432 MsaT012458.1:CDS
AAGTGACAATAAAACATCAC+TGG 0.527333 3:+25042209 MsaT012458.1:CDS
ATGCATCCCAACGTTGATAA+CGG 0.547251 3:+25042045 MsaT012458.1:CDS
TGAAGCACCGTTATCAACGT+TGG 0.555762 3:-25042052 None:intergenic
CTTCGGAAAATCTACGCGGT+TGG 0.564527 3:+25043425 MsaT012458.1:CDS
AAACATAGATATGACTATTG+AGG 0.565786 3:+25042122 MsaT012458.1:CDS
CAGGTACATGTTAAAGATGG+TGG 0.568652 3:+25043336 MsaT012458.1:intron
GAAGGACAAAGTAAACTTTG+AGG 0.573836 3:+25043450 MsaT012458.1:CDS
CGACAGGTACATGTTAAAGA+TGG 0.589384 3:+25043333 MsaT012458.1:intron
ACAATTGAAAAGAAAGACAA+TGG 0.608503 3:+25042233 MsaT012458.1:CDS
GCCAAATATGAAGAAGAATG+TGG 0.626878 3:+25043493 MsaT012458.1:CDS
TGCAAAACAAGATAACGGAA+AGG 0.654316 3:+25042080 MsaT012458.1:CDS
ACAATGGCTCGTGCACAAGA+GGG 0.658700 3:+25042249 MsaT012458.1:CDS
TATGGAGTAGTACCTGACAA+TGG 0.702873 3:+25043385 MsaT012458.1:CDS
GGCTCTTCGGAAAATCTACG+CGG 0.742332 3:+25043421 MsaT012458.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 25042045 25049018 25042045 ID=MsaG012458
Chr3 mRNA 25042045 25049018 25042045 ID=MsaT012458.1;Parent=MsaG012458
Chr3 exon 25042045 25042280 25042045 ID=MsaT012458.1.exon1;Parent=MsaT012458.1
Chr3 CDS 25042045 25042280 25042045 ID=cds.MsaT012458.1;Parent=MsaT012458.1
Chr3 exon 25043339 25043514 25043339 ID=MsaT012458.1.exon2;Parent=MsaT012458.1
Chr3 CDS 25043339 25043514 25043339 ID=cds.MsaT012458.1;Parent=MsaT012458.1
Chr3 exon 25049005 25049018 25049005 ID=MsaT012458.1.exon3;Parent=MsaT012458.1
Chr3 CDS 25049005 25049018 25049005 ID=cds.MsaT012458.1;Parent=MsaT012458.1
Gene Sequence

>MsaT012458.1

ATGCATCCCAACGTTGATAACGGTGCTTCAAACAATGCAAAACAAGATAACGGAAAGGAAAAAGAAGAAGGTGGTGAAAACATAGATATGACTATTGAGGAACTAGAGACAAAGATATGGAAAGATAAAATGCTCTTAAAGAAGATGAAGTTAAAAAGAATTCAAAGTGACAATAAAACATCACTGGAACAATTGAAAAGAAAGACAATGGCTCGTGCACAAGAGGGCATATTAAGGTACATGTTAAAGATGGTGGAATTTTGTGATGTTCGTGGCTTCGTCTATGGAGTAGTACCTGACAATGGCAAACCAATGATTGGCTCTTCGGAAAATCTACGCGGTTGGTGGAAGGACAAAGTAAACTTTGAGGAAAACGGTCCAGCTGCAATCGCCAAATATGAAGAAGAATGTGAGATGGGAAACTAA

Protein sequence

>MsaT012458.1

MHPNVDNGASNNAKQDNGKEKEEGGENIDMTIEELETKIWKDKMLLKKMKLKRIQSDNKTSLEQLKRKTMARAQEGILRYMLKMVEFCDVRGFVYGVVPDNGKPMIGSSENLRGWWKDKVNFEENGPAAIAKYEEECEMGN*