Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012495 | XP_039687084.1 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 4.63e-66 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012495 | G7INX6 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 2.21e-66 | 205 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG012495 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG012495 | MsaG017279 | 0.804529 | 3.490590e-49 | 5.234343e-47 |
| MsaG012495 | MsaG028949 | 0.804671 | 3.261390e-49 | 4.906781e-47 |
| MsaG012495 | MsaG042118 | 0.807766 | 7.320849e-50 | 1.184690e-47 |
| MsaG012495 | MsaG042123 | 0.805686 | 2.004191e-49 | 3.087346e-47 |
| MsaG012495 | MsaG044316 | 0.818026 | 4.234922e-52 | 8.845224e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG012495 | MtrunA17_Chr2g0281511 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 4.26e-70 | 205 |
| MsaG012495 | MtrunA17_Chr2g0309121 | 81.667 | 120 | 21 | 1 | 1 | 120 | 1 | 119 | 2.66e-67 | 198 |
| MsaG012495 | MtrunA17_Chr3g0081891 | 81.250 | 80 | 15 | 0 | 1 | 80 | 1 | 80 | 7.69e-43 | 135 |
| MsaG012495 | MtrunA17_Chr7g0276661 | 33.588 | 131 | 67 | 4 | 7 | 119 | 38 | 166 | 9.19e-18 | 74.3 |
| MsaG012495 | MtrunA17_Chr0c01g0489371 | 40.659 | 91 | 52 | 1 | 22 | 110 | 224 | 314 | 1.53e-15 | 70.9 |
| MsaG012495 | MtrunA17_Chr8g0356961 | 40.449 | 89 | 51 | 1 | 24 | 110 | 77 | 165 | 4.73e-15 | 67.4 |
| MsaG012495 | MtrunA17_Chr8g0372431 | 27.835 | 97 | 70 | 0 | 24 | 120 | 6 | 102 | 6.67e-15 | 65.5 |
| MsaG012495 | MtrunA17_Chr5g0430431 | 35.955 | 89 | 47 | 1 | 37 | 115 | 165 | 253 | 2.20e-13 | 64.3 |
| MsaG012495 | MtrunA17_Chr8g0356951 | 34.653 | 101 | 62 | 3 | 24 | 120 | 217 | 317 | 2.88e-11 | 58.9 |
| MsaG012495 | MtrunA17_Chr5g0434641 | 34.831 | 89 | 56 | 1 | 24 | 110 | 210 | 298 | 5.47e-11 | 58.2 |
| MsaG012495 | MtrunA17_Chr7g0228081 | 30.000 | 80 | 56 | 0 | 41 | 120 | 13 | 92 | 6.05e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTCAACGGTCCTGAATT+AGG | 0.221297 | 3:+25838698 | MsaT012495.1:CDS |
| AAAATGTCGATGTTCATTAT+TGG | 0.262235 | 3:+25838729 | MsaT012495.1:CDS |
| TACCAGTTTCTTCGTCAAAA+AGG | 0.277417 | 3:-25839376 | None:intergenic |
| TAATGTTATTGCTTCTACTT+TGG | 0.311699 | 3:+25838757 | MsaT012495.1:CDS |
| GTAGCATACATCCTATTAAA+AGG | 0.335252 | 3:-25839530 | None:intergenic |
| TTTGGATGGAAACAATGTTT+TGG | 0.360997 | 3:+25838775 | MsaT012495.1:CDS |
| GTACGGCTTTGTTCTTGAAA+AGG | 0.418940 | 3:+25839466 | MsaT012495.1:CDS |
| AAGTCAACGGTCCTGAATTA+GGG | 0.429565 | 3:+25838699 | MsaT012495.1:CDS |
| GTTATTGCTTCTACTTTGGA+TGG | 0.442931 | 3:+25838761 | MsaT012495.1:CDS |
| TACGGCTTTGTTCTTGAAAA+GGG | 0.445793 | 3:+25839467 | MsaT012495.1:CDS |
| GCATGCATCAGAAGTACATA+GGG | 0.496344 | 3:+25839435 | MsaT012495.1:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 3:+25839479 | MsaT012495.1:CDS |
| AGCATGCATCAGAAGTACAT+AGG | 0.546367 | 3:+25839434 | MsaT012495.1:CDS |
| CATCAGAAGTACATAGGGAG+AGG | 0.578101 | 3:+25839440 | MsaT012495.1:CDS |
| AGAAGTACATAGGGAGAGGA+TGG | 0.580923 | 3:+25839444 | MsaT012495.1:CDS |
| TACATAGGGAGAGGATGGTA+CGG | 0.597869 | 3:+25839449 | MsaT012495.1:CDS |
| ACATTCTCAGCAACACCTCG+AGG | 0.684927 | 3:-25839326 | None:intergenic |
| GAGACTGGAGTTGAAGTCAA+CGG | 0.686388 | 3:+25838686 | MsaT012495.1:CDS |
| GAATTAGTCTCAGTTAACGA+CGG | 0.726011 | 3:-25839552 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 25838680 | 25839565 | 25838680 | ID=MsaG012495 |
| Chr3 | mRNA | 25838680 | 25839565 | 25838680 | ID=MsaT012495.1;Parent=MsaG012495 |
| Chr3 | exon | 25838680 | 25838796 | 25838680 | ID=MsaT012495.1.exon1;Parent=MsaT012495.1 |
| Chr3 | CDS | 25838680 | 25838796 | 25838680 | ID=cds.MsaT012495.1;Parent=MsaT012495.1 |
| Chr3 | exon | 25839320 | 25839565 | 25839320 | ID=MsaT012495.1.exon2;Parent=MsaT012495.1 |
| Chr3 | CDS | 25839320 | 25839565 | 25839320 | ID=cds.MsaT012495.1;Parent=MsaT012495.1 |
| Gene Sequence |
| Protein sequence |
