AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012502

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012502 MtrunA17_Chr3g0080761 96.241 665 21 3 1 662 1 664 0.0 1327
MsaG012502 MtrunA17_Chr8g0354821 59.797 689 230 8 15 661 4 687 0.0 847
MsaG012502 MtrunA17_Chr7g0255181 46.757 740 279 20 1 661 1 704 0.0 629
MsaG012502 MtrunA17_Chr5g0399801 41.450 538 294 13 125 659 567 1086 6.47e-122 388
MsaG012502 MtrunA17_Chr4g0003501 40.414 532 293 15 133 661 444 954 2.10e-113 363
MsaG012502 MtrunA17_Chr4g0003511 39.630 540 304 14 125 661 531 1051 3.42e-112 362
MsaG012502 MtrunA17_Chr8g0368911 40.546 476 251 12 172 631 290 749 3.67e-110 350
MsaG012502 MtrunA17_Chr8g0368901 41.239 468 255 12 174 631 299 756 1.50e-109 348
MsaG012502 MtrunA17_Chr1g0163051 37.058 537 299 14 108 631 135 645 1.42e-106 337
MsaG012502 MtrunA17_Chr8g0369301 40.179 448 247 9 194 631 300 736 4.80e-104 333
MsaG012502 MtrunA17_Chr6g0476111 36.914 512 283 15 171 658 195 690 5.27e-96 310
MsaG012502 MtrunA17_Chr6g0476121 36.133 512 287 15 171 658 144 639 2.45e-93 301
MsaG012502 MtrunA17_Chr7g0252211 36.686 507 296 12 169 658 255 753 9.97e-92 300
MsaG012502 MtrunA17_Chr1g0163041 34.766 512 300 14 139 631 68 564 5.02e-91 294
MsaG012502 MtrunA17_Chr6g0467381 34.586 532 285 13 139 633 60 565 5.97e-90 291
MsaG012502 MtrunA17_Chr6g0467531 35.743 498 273 12 157 633 94 565 1.68e-89 290
MsaG012502 MtrunA17_Chr6g0467421 34.398 532 286 13 139 633 60 565 2.12e-89 289
MsaG012502 MtrunA17_Chr6g0467401 34.398 532 286 13 139 633 60 565 7.13e-88 285
MsaG012502 MtrunA17_Chr3g0094881 35.098 510 291 14 135 633 144 624 2.03e-84 278
MsaG012502 MtrunA17_Chr6g0467411 32.596 497 273 13 155 633 31 483 2.02e-71 239
MsaG012502 MtrunA17_Chr2g0306961 31.836 512 276 11 139 633 9 464 3.95e-70 235
MsaG012502 MtrunA17_Chr1g0170651 33.809 491 264 12 189 661 98 545 7.72e-67 229
MsaG012502 MtrunA17_Chr7g0232811 29.508 549 272 19 135 657 75 534 4.31e-60 210
MsaG012502 MtrunA17_Chr7g0232981 29.434 530 274 18 135 657 75 511 4.18e-55 196
MsaG012502 MtrunA17_Chr7g0232841 28.650 548 264 21 135 657 78 523 1.25e-51 187
MsaG012502 MtrunA17_Chr1g0190311 27.811 338 171 10 372 658 325 640 6.10e-32 131
MsaG012502 MtrunA17_Chr5g0403241 31.889 323 166 10 370 661 1207 1506 1.34e-31 132
MsaG012502 MtrunA17_Chr1g0183321 28.487 337 169 10 372 658 428 742 1.14e-28 122
MsaG012502 MtrunA17_Chr8g0373821 28.923 325 177 9 359 657 237 533 1.69e-28 120
MsaG012502 MtrunA17_Chr4g0075321 34.685 222 113 5 445 661 869 1063 4.00e-27 117
MsaG012502 MtrunA17_Chr7g0262001 27.795 331 173 7 374 658 388 698 1.54e-25 112
MsaG012502 MtrunA17_Chr1g0201621 30.964 197 97 7 462 657 61 219 4.63e-16 81.3
MsaG012502 MtrunA17_Chr1g0201311 30.457 197 98 6 462 657 61 219 6.36e-16 80.9
MsaG012502 MtrunA17_Chr6g0477001 27.551 196 99 4 459 645 87 248 2.07e-15 78.2
MsaG012502 MtrunA17_Chr7g0232751 36.937 111 63 3 549 657 9 114 4.38e-15 72.0
MsaG012502 MtrunA17_Chr2g0305371 30.457 197 96 5 463 659 314 469 7.36e-15 77.8
MsaG012502 MtrunA17_Chr2g0297191 27.933 179 90 5 483 661 1031 1170 1.10e-12 71.6
MsaG012502 MtrunA17_Chr7g0232761 32.867 143 77 6 192 329 141 269 7.62e-12 66.6
MsaG012502 MtrunA17_Chr7g0232871 38.667 75 39 2 477 547 16 87 2.74e-11 60.8
MsaG012502 MtrunA17_Chr1g0149581 28.409 176 93 4 486 661 1523 1665 4.91e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012502 AT5G04940.2 56.588 592 237 7 70 661 98 669 0.0 691
MsaG012502 AT5G04940.1 56.588 592 237 7 70 661 98 669 0.0 691
MsaG012502 AT1G73100.1 51.180 678 294 9 6 661 6 668 0.0 686
MsaG012502 AT1G17770.1 43.351 579 295 12 98 662 134 693 1.60e-154 462
MsaG012502 AT2G24740.1 45.102 541 251 14 129 661 252 754 9.56e-148 447
MsaG012502 AT2G35160.5 37.797 590 309 19 80 661 218 757 8.37e-111 351
MsaG012502 AT2G35160.2 37.797 590 309 19 80 661 254 793 3.10e-110 351
MsaG012502 AT2G35160.3 37.797 590 309 19 80 661 254 793 3.10e-110 351
MsaG012502 AT2G35160.1 37.797 590 309 19 80 661 254 793 3.10e-110 351
MsaG012502 AT2G35160.4 37.797 590 309 19 80 661 254 793 3.10e-110 351
MsaG012502 AT4G13460.1 39.010 505 284 11 139 631 145 637 3.65e-107 338
MsaG012502 AT4G13460.2 39.010 505 284 11 139 631 145 637 3.65e-107 338
MsaG012502 AT2G33290.1 38.583 508 279 8 139 631 149 638 1.96e-105 334
MsaG012502 AT2G33290.3 38.583 508 279 8 139 631 149 638 1.96e-105 334
MsaG012502 AT2G33290.4 38.583 508 279 8 139 631 149 638 1.96e-105 334
MsaG012502 AT2G33290.2 38.583 508 279 8 139 631 149 638 1.96e-105 334
MsaG012502 AT2G22740.2 37.523 533 304 13 139 658 270 786 3.46e-105 337
MsaG012502 AT2G22740.1 37.523 533 304 13 139 658 270 786 3.46e-105 337
MsaG012502 AT5G13960.2 36.140 487 279 14 192 658 12 486 1.79e-90 290
MsaG012502 AT5G13960.1 35.225 511 299 14 168 658 122 620 2.31e-89 291
MsaG012502 AT2G05900.1 41.176 221 121 4 212 428 1 216 4.93e-45 163
MsaG012502 AT2G05900.1 54.118 85 30 1 577 661 236 311 8.21e-23 100
MsaG012502 AT5G47150.1 41.589 214 113 5 139 347 118 324 2.53e-41 153
MsaG012502 AT3G04380.2 27.463 335 175 8 372 658 117 431 2.74e-35 139
MsaG012502 AT3G04380.3 27.463 335 175 8 372 658 128 442 3.07e-35 139
MsaG012502 AT3G04380.5 27.463 335 175 8 372 658 101 415 3.70e-35 139
MsaG012502 AT3G04380.6 27.463 335 175 8 372 658 117 431 3.73e-35 139
MsaG012502 AT3G04380.1 27.463 335 175 8 372 658 144 458 3.90e-35 139
MsaG012502 AT3G04380.4 27.463 335 175 8 372 658 144 458 3.97e-35 139
MsaG012502 AT5G47160.1 37.549 253 137 8 103 344 166 408 1.90e-34 136
MsaG012502 AT5G47160.3 37.549 253 137 8 103 344 166 408 1.90e-34 136
MsaG012502 AT5G47160.2 37.549 253 137 8 103 344 166 408 1.90e-34 136
MsaG012502 AT2G23740.1 31.988 322 166 15 368 662 1080 1375 8.51e-32 133
MsaG012502 AT2G23740.3 31.988 322 166 15 368 662 1087 1382 1.00e-31 132
MsaG012502 AT2G23740.6 31.988 322 166 15 368 662 1087 1382 1.00e-31 132
MsaG012502 AT2G23740.2 31.988 322 166 15 368 662 1087 1382 1.00e-31 132
MsaG012502 AT1G04050.3 29.674 337 163 13 372 658 303 615 5.23e-30 126
MsaG012502 AT1G04050.2 30.267 337 161 14 372 658 407 719 6.03e-30 126
MsaG012502 AT1G04050.1 30.267 337 161 14 372 658 407 719 6.03e-30 126
MsaG012502 AT5G43990.16 28.190 337 165 12 372 658 373 682 9.10e-29 122
MsaG012502 AT5G43990.5 28.190 337 165 12 372 658 373 682 9.10e-29 122
MsaG012502 AT5G43990.17 28.190 337 165 12 372 658 373 682 9.10e-29 122
MsaG012502 AT5G43990.14 28.190 337 165 12 372 658 373 682 9.10e-29 122
MsaG012502 AT5G43990.8 28.190 337 165 12 372 658 324 633 9.24e-29 122
MsaG012502 AT5G43990.12 28.190 337 165 12 372 658 324 633 9.24e-29 122
MsaG012502 AT5G43990.6 28.190 337 165 12 372 658 324 633 9.24e-29 122
MsaG012502 AT5G43990.13 28.190 337 165 12 372 658 324 633 9.24e-29 122
MsaG012502 AT5G43990.3 28.190 337 165 12 372 658 393 702 9.57e-29 122
MsaG012502 AT5G43990.11 28.190 337 165 12 372 658 393 702 9.57e-29 122
MsaG012502 AT5G43990.1 28.190 337 165 12 372 658 393 702 9.57e-29 122
MsaG012502 AT5G43990.4 28.190 337 165 12 372 658 393 702 9.57e-29 122
MsaG012502 AT5G43990.15 28.190 337 165 12 372 658 396 705 1.03e-28 122
MsaG012502 AT5G43990.10 28.190 337 165 12 372 658 396 705 1.03e-28 122
MsaG012502 AT5G43990.7 28.190 337 165 12 372 658 304 613 1.09e-28 122
MsaG012502 AT5G43990.9 28.190 337 165 12 372 658 416 725 1.19e-28 122
MsaG012502 AT5G43990.2 28.190 337 165 12 372 658 416 725 1.19e-28 122
MsaG012502 AT3G03750.2 33.188 229 127 8 435 661 145 349 2.62e-23 102
MsaG012502 AT2G23740.4 32.530 249 120 14 368 589 1087 1314 1.71e-20 97.4
MsaG012502 AT2G23740.5 32.530 249 120 14 368 589 1087 1314 1.71e-20 97.4
MsaG012502 AT3G03750.1 29.956 227 121 8 435 661 145 333 7.05e-17 82.8
MsaG012502 AT1G77300.2 30.928 194 97 6 466 659 1008 1164 2.30e-15 80.9
MsaG012502 AT1G77300.1 30.928 194 97 6 466 659 1008 1164 3.57e-15 80.5
MsaG012502 AT4G30860.1 29.442 197 98 5 463 659 307 462 1.65e-13 73.9
MsaG012502 AT3G59960.3 27.869 183 75 6 485 658 112 246 9.43e-12 66.6
MsaG012502 AT3G59960.2 27.869 183 75 6 485 658 112 246 9.43e-12 66.6
MsaG012502 AT3G59960.4 27.869 183 75 6 485 658 112 246 9.43e-12 66.6
MsaG012502 AT3G59960.1 28.814 177 81 6 485 650 112 254 2.58e-11 65.9

Find 166 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAGGTCAGTATCCTTC+TGG 0.204050 3:-25951182 MsaT012502.1:CDS
ACAGGTATCAAACTTCTTAT+AGG 0.235001 3:+25951230 None:intergenic
CGACTCCACTTCAAGCATTT+AGG 0.258428 3:-25951091 MsaT012502.1:CDS
CAAGCATTTAGGAGCCCTTT+TGG 0.274971 3:-25951080 MsaT012502.1:CDS
CATTGACAAGTATAAGATTC+TGG 0.293840 3:-25951261 MsaT012502.1:CDS
AGAGTGGAAATATGTGAAAA+AGG 0.306165 3:+25950116 None:intergenic
TGCATTAAACTGAATGATTT+CGG 0.306178 3:+25950084 None:intergenic
CATGAGTTAACATATGATTA+TGG 0.312052 3:-25949436 MsaT012502.1:CDS
TTATATTATCAATGGAGTTC+TGG 0.315928 3:-25949968 MsaT012502.1:CDS
AGAACTCCATTGATAATATA+AGG 0.322628 3:+25949970 None:intergenic
TGTATAAAATTCAAGATTCA+TGG 0.343226 3:-25950350 MsaT012502.1:CDS
TTCTTAGCATTTATAATAAG+AGG 0.346128 3:+25949592 None:intergenic
AGCATGTGTCCCTGGCGATT+TGG 0.346957 3:-25950028 MsaT012502.1:CDS
CGAATGGAAATGTGTGTTAT+CGG 0.357207 3:-25950681 MsaT012502.1:CDS
CTTCAGGTCAGTATCCTTCT+GGG 0.359790 3:-25951181 MsaT012502.1:CDS
AACCTAGTGCCTTTGCCGTT+TGG 0.360454 3:-25950266 MsaT012502.1:CDS
TTTGGTGCTTCAAACCGGTT+TGG 0.365259 3:+25949872 None:intergenic
TCATAAGGGTAATCTTGCTT+TGG 0.385063 3:-25950466 MsaT012502.1:CDS
CAAAACAAAGGATAAAGGTT+GGG 0.388648 3:-25949839 MsaT012502.1:CDS
TTGGTGCTTCAAACCGGTTT+GGG 0.391461 3:+25949873 None:intergenic
AATCCTCAAGGTCAATCTTC+AGG 0.392097 3:-25951197 MsaT012502.1:CDS
TGTTATAATTTATACCGGTC+AGG 0.396575 3:-25950535 MsaT012502.1:CDS
ACTTCATTGACAAGTGATAC+TGG 0.399879 3:+25950159 None:intergenic
TGTGTTTGTTCGAATTCCTT+TGG 0.402301 3:+25950758 None:intergenic
GATTGATGCCTTGCATATCC+AGG 0.403598 3:-25950634 MsaT012502.1:CDS
ACTTGAAAGATTCATAAATA+CGG 0.407062 3:+25949686 None:intergenic
CGAACCTGTGTTCCATTCCT+TGG 0.409287 3:+25950821 None:intergenic
CAGTACCTGGAGTTGAGATT+GGG 0.418871 3:-25950719 MsaT012502.1:CDS
ACAGGCTGCCAGAAAACATT+TGG 0.419329 3:+25949529 None:intergenic
ATTTGGACTGCTCATGCATT+CGG 0.422721 3:-25950011 MsaT012502.1:CDS
TACCATTGTCTCTTTGACCT+GGG 0.423334 3:+25950916 None:intergenic
TCAGTACCTGGAGTTGAGAT+TGG 0.424381 3:-25950720 MsaT012502.1:CDS
TTCCATTTGGTGCTTCAAAC+CGG 0.425045 3:+25949867 None:intergenic
ATTATAAATGCTAAGAATGT+TGG 0.437154 3:-25949586 MsaT012502.1:CDS
CATCGCTCTACTTGGCTTTG+CGG 0.438113 3:+25951115 None:intergenic
GCTGTTAGCATTGTTTCGTC+AGG 0.439867 3:-25950588 MsaT012502.1:CDS
AGAATAAGTCCAGTCCTTGC+AGG 0.449322 3:+25950213 None:intergenic
AAGGTTGGGGTCTTCGATCA+TGG 0.452431 3:-25949825 MsaT012502.1:CDS
GCTGGTTTCCCTGCAAGGAC+TGG 0.456055 3:-25950222 MsaT012502.1:CDS
ATAAAAGCACCAGCACGAAT+AGG 0.460396 3:+25949799 None:intergenic
TTACCATTGTCTCTTTGACC+TGG 0.461172 3:+25950915 None:intergenic
GTGTTCAAAACAAAGGATAA+AGG 0.462890 3:-25949844 MsaT012502.1:CDS
GTGAATTGTATAATGGAAGA+AGG 0.464015 3:-25951317 None:intergenic
CGGTTTGCATGCTCAATCGA+TGG 0.470289 3:-25950661 MsaT012502.1:CDS
CGATAACACACATTTCCATT+CGG 0.476797 3:+25950682 None:intergenic
TATTCTTGCTGACCTCTCCT+CGG 0.477270 3:-25950196 MsaT012502.1:CDS
AGATCCTCATCCTCTCCAAA+AGG 0.481832 3:+25951065 None:intergenic
GTATCATGAGGTCCACCAAA+TGG 0.483316 3:+25951143 None:intergenic
ATCCTCACTAGACTCGTTTG+TGG 0.484407 3:+25949630 None:intergenic
CTTGAAAGATTCATAAATAC+GGG 0.486596 3:+25949687 None:intergenic
GTCAGATCAGAAGCTTCATA+AGG 0.487005 3:-25950481 MsaT012502.1:CDS
CATGAGCAGTCCAAATCGCC+AGG 0.488074 3:+25950018 None:intergenic
GTTGATATGAGTGGTTTAGT+TGG 0.492160 3:-25950945 MsaT012502.1:CDS
ACTGCTACCCTCGGTATGAT+TGG 0.492178 3:+25949402 None:intergenic
TGTATAATGGAAGAAGGGTT+AGG 0.492317 3:-25951311 None:intergenic
CAGAATCTTATACTTGTCAA+TGG 0.495015 3:+25951262 None:intergenic
GCACAATATTAACAACTTCA+CGG 0.500326 3:+25950892 None:intergenic
TGCATATCCAGGGTGACCGC+GGG 0.500357 3:-25950623 MsaT012502.1:CDS
TCACAGCATCTTTGATGCCT+CGG 0.503981 3:+25950406 None:intergenic
GGTTTGCATGCTCAATCGAT+GGG 0.505325 3:-25950660 MsaT012502.1:CDS
TATCATGAGGTCCACCAAAT+GGG 0.505449 3:+25951144 None:intergenic
TTGTTCTCTTCGTATAAGAC+AGG 0.505552 3:+25949511 None:intergenic
AACCGGTTTGAAGCACCAAA+TGG 0.508329 3:-25949869 MsaT012502.1:CDS
TCTGTTCAGAAGTGGAAAGC+TGG 0.508734 3:-25950240 MsaT012502.1:CDS
ATCAAAGATGCTGTGACTCC+AGG 0.511194 3:-25950399 MsaT012502.1:CDS
GGTGATGTTATAATTTATAC+CGG 0.512209 3:-25950540 MsaT012502.1:CDS
TATCTTCCCCGCGGTCACCC+TGG 0.516259 3:+25950616 None:intergenic
ATTCTTGCTGACCTCTCCTC+GGG 0.517115 3:-25950195 MsaT012502.1:CDS
TCAATGGAGTTCTGGCGAAT+CGG 0.519477 3:-25949960 MsaT012502.1:CDS
GTATAAAATTCAAGATTCAT+GGG 0.521482 3:-25950349 MsaT012502.1:CDS
TATAAGTTGATAAGAGTGCC+TGG 0.523531 3:-25950291 MsaT012502.1:CDS
TCAACACTCAGTTCCTCCCC+CGG 0.526455 3:-25951288 MsaT012502.1:CDS
TGACCGCGGGGAAGATACTT+TGG 0.527412 3:-25950610 MsaT012502.1:CDS
GGTTTAGTTGGTATAAGCCC+AGG 0.529198 3:-25950933 MsaT012502.1:CDS
AAACGTACCGATTTAAAAGC+TGG 0.530169 3:-25950795 MsaT012502.1:CDS
GTTGAAATTCCCACCCTGAC+CGG 0.530593 3:+25950521 None:intergenic
TCATATGTTAACTCATGCAT+TGG 0.532861 3:+25949442 None:intergenic
CGGCAGCATCACATTCAAGC+CGG 0.533696 3:-25951007 MsaT012502.1:CDS
TCAAAACAAAGGATAAAGGT+TGG 0.534159 3:-25949840 MsaT012502.1:CDS
GGTAGAAAGAAATGCTTATG+TGG 0.534383 3:-25949370 MsaT012502.1:CDS
TCATGGGATCCTATTCGTGC+TGG 0.535655 3:-25949808 MsaT012502.1:CDS
TCAGATCAGAAGCTTCATAA+GGG 0.535931 3:-25950480 MsaT012502.1:CDS
TGCATGCTCAATCGATGGGG+GGG 0.538668 3:-25950656 MsaT012502.1:CDS
TGAATTGTATAATGGAAGAA+GGG 0.540125 3:-25951316 None:intergenic
GGAGTCGGCATCGCTCTACT+TGG 0.540336 3:+25951107 None:intergenic
AGGTTGGGGTCTTCGATCAT+GGG 0.540693 3:-25949824 MsaT012502.1:CDS
AAAACAAAGGATAAAGGTTG+GGG 0.541211 3:-25949838 MsaT012502.1:CDS
GGCAATATTTGTATGACCAA+AGG 0.542268 3:-25950774 MsaT012502.1:CDS
GGAAAGCTGGTTTCCCTGCA+AGG 0.545796 3:-25950227 MsaT012502.1:CDS
GACTCTCGGCTCCCGAGGAG+AGG 0.546001 3:+25950184 None:intergenic
ATCTCAACTTGTAGATGCCA+AGG 0.547043 3:-25950838 MsaT012502.1:CDS
ATGCAACATGGAGAAAGGAT+TGG 0.548940 3:+25949488 None:intergenic
TTCCAAACGGCAAAGGCACT+AGG 0.550397 3:+25950264 None:intergenic
GATCCTCATCCTCTCCAAAA+GGG 0.552939 3:+25951066 None:intergenic
AACTTGTAGATGCCAAGGAA+TGG 0.553451 3:-25950833 MsaT012502.1:CDS
TTTGGAAATCTGTTCAGAAG+TGG 0.553589 3:-25950248 MsaT012502.1:CDS
ATGTCCCCAATCTCAACTCC+AGG 0.554821 3:+25950714 None:intergenic
TCTTAGCATTTATAATAAGA+GGG 0.554977 3:+25949593 None:intergenic
GGCAGCATCACATTCAAGCC+GGG 0.555680 3:-25951006 MsaT012502.1:CDS
GTATTTATGAATCTTTCAAG+TGG 0.556979 3:-25949684 MsaT012502.1:CDS
GCAAAGGCACTAGGTTGTCC+AGG 0.558915 3:+25950273 None:intergenic
TTATACTTGTCAATGGAACC+CGG 0.562427 3:+25951269 None:intergenic
GGTCAGATTGAAGAAGCATA+AGG 0.562915 3:-25950985 MsaT012502.1:CDS
TCACATAGTTGCAGCTGTAG+TGG 0.565201 3:-25950057 MsaT012502.1:CDS
GCATTTATAATAAGAGGGTG+TGG 0.566087 3:+25949598 None:intergenic
CTAAATGCTTGAAGTGGAGT+CGG 0.566439 3:+25951092 None:intergenic
GAGAAGTTATCGATAAAGCT+AGG 0.566713 3:-25949762 MsaT012502.1:CDS
GTTATAATTTATACCGGTCA+GGG 0.567735 3:-25950534 MsaT012502.1:CDS
TTGCATGCTCAATCGATGGG+GGG 0.569075 3:-25950657 MsaT012502.1:CDS
ATTTCGGATGTCTAAGAGAG+TGG 0.569188 3:+25950100 None:intergenic
AAGCCGTTGGTTCATGAATG+TGG 0.569854 3:-25949937 MsaT012502.1:CDS
TTTGCATGCTCAATCGATGG+GGG 0.571740 3:-25950658 MsaT012502.1:CDS
GAGGATCTCAATGACAATGA+TGG 0.572099 3:-25951050 MsaT012502.1:CDS
TATGCTTCTTCAATCTGACC+CGG 0.573333 3:+25950988 None:intergenic
GTCGCTTCTTCTAGTAACTT+AGG 0.574919 3:+25949652 None:intergenic
TCGAACAAACACAGCGAAGA+GGG 0.575127 3:-25950748 MsaT012502.1:CDS
TTGCATATCCAGGGTGACCG+CGG 0.575259 3:-25950624 MsaT012502.1:CDS
TTTGCGTGCACTGCTACCCT+CGG 0.576882 3:+25949393 None:intergenic
TATGTTAACTCATGCATTGG+TGG 0.577942 3:+25949445 None:intergenic
GTTTGCATGCTCAATCGATG+GGG 0.580565 3:-25950659 MsaT012502.1:CDS
AACAGATTTCCAAACGGCAA+AGG 0.589578 3:+25950257 None:intergenic
GGGCTCCTAAATGCTTGAAG+TGG 0.589829 3:+25951086 None:intergenic
TTAAGTCAGCTTGTACAAGA+AGG 0.590145 3:-25949739 MsaT012502.1:CDS
AATGGAAGTGTTCAAAACAA+AGG 0.598701 3:-25949851 MsaT012502.1:CDS
AAGTGATACTGGAAGACTCT+CGG 0.599204 3:+25950170 None:intergenic
CTTCTGAACAGATTTCCAAA+CGG 0.599772 3:+25950251 None:intergenic
TATACTTGTCAATGGAACCC+GGG 0.600215 3:+25951270 None:intergenic
TGACCTGAAGATTGACCTTG+AGG 0.600787 3:+25951194 None:intergenic
GAATAAGTCCAGTCCTTGCA+GGG 0.601962 3:+25950214 None:intergenic
CATTTGATGCGCTAAGACGA+AGG 0.602005 3:-25950863 MsaT012502.1:CDS
AAGAGGGTAGGATCAGTACC+TGG 0.602661 3:-25950732 MsaT012502.1:CDS
GGGCCACATTCATGAACCAA+CGG 0.610204 3:+25949934 None:intergenic
TCTGGCGAATCGGAAGCCGT+TGG 0.612003 3:-25949950 MsaT012502.1:CDS
TGCTAAGAATGTTGGAAATG+TGG 0.613562 3:-25949578 MsaT012502.1:CDS
GATGTTCATGTTGATATGAG+TGG 0.616806 3:-25950954 MsaT012502.1:CDS
TGGAAGACTCTCGGCTCCCG+AGG 0.617476 3:+25950179 None:intergenic
AGCCCAGGTCAAAGAGACAA+TGG 0.619161 3:-25950918 MsaT012502.1:CDS
TAATTTATACCGGTCAGGGT+GGG 0.620279 3:-25950530 MsaT012502.1:CDS
TTAGGAAAACACTGACATGT+GGG 0.620821 3:+25949913 None:intergenic
GACCGTTCCAATCATACCGA+GGG 0.625961 3:-25949409 MsaT012502.1:CDS
TACGACGATGAAGCTGAGGA+TGG 0.627250 3:-25950561 MsaT012502.1:CDS
TACCCTCGGTATGATTGGAA+CGG 0.630497 3:+25949407 None:intergenic
GCGGTCACCCTGGATATGCA+AGG 0.632039 3:+25950626 None:intergenic
ACAAACACAGCGAAGAGGGT+AGG 0.634440 3:-25950744 MsaT012502.1:CDS
CTACAGCTGCAACTATGTGA+AGG 0.634477 3:+25950060 None:intergenic
ATACTTGTCAATGGAACCCG+GGG 0.636158 3:+25951271 None:intergenic
ATTGATGCCTTGCATATCCA+GGG 0.637842 3:-25950633 MsaT012502.1:CDS
GTTAGGAAAACACTGACATG+TGG 0.639912 3:+25949912 None:intergenic
GATGCCAAGGAATGGAACAC+AGG 0.640965 3:-25950825 MsaT012502.1:CDS
AGAATACGACGATGAAGCTG+AGG 0.645222 3:-25950565 MsaT012502.1:CDS
TTCGAACAAACACAGCGAAG+AGG 0.646517 3:-25950749 MsaT012502.1:CDS
TTTGCGGTTGAAGTATCATG+AGG 0.649080 3:+25951131 None:intergenic
AGTACCTGGAGTTGAGATTG+GGG 0.657916 3:-25950718 MsaT012502.1:CDS
TCATGCATTCGGAGAAACGA+AGG 0.659918 3:-25950000 MsaT012502.1:CDS
ATAATTTATACCGGTCAGGG+TGG 0.675305 3:-25950531 MsaT012502.1:CDS
GAGGGTAGCAGTGCACGCAA+AGG 0.681126 3:-25949391 MsaT012502.1:CDS
TGTGGATCATCAAACTGCCG+CGG 0.682422 3:-25949352 MsaT012502.1:CDS
GACCACAAACGAGTCTAGTG+AGG 0.682671 3:-25949632 MsaT012502.1:CDS
ATGAGCAGTCCAAATCGCCA+GGG 0.685642 3:+25950019 None:intergenic
TAGGAAAACACTGACATGTG+GGG 0.688446 3:+25949914 None:intergenic
TGACCGTTCCAATCATACCG+AGG 0.697564 3:-25949410 MsaT012502.1:CDS
GCATATCCAGGGTGACCGCG+GGG 0.702296 3:-25950622 MsaT012502.1:CDS
AATGTCAAGTAAAAGAACCG+CGG 0.738828 3:+25949335 None:intergenic
AATGAAATAAGAGTCATCCG+AGG 0.740934 3:-25950423 MsaT012502.1:CDS
CAGCCAAAGTATCTTCCCCG+CGG 0.752822 3:+25950607 None:intergenic
TACTTGTCAATGGAACCCGG+GGG 0.840303 3:+25951272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 25949339 25951327 25949339 ID=MsaG012502
Chr3 mRNA 25949339 25951327 25949339 ID=MsaT012502.1;Parent=MsaG012502
Chr3 exon 25949339 25951327 25949339 ID=MsaT012502.1.exon1;Parent=MsaT012502.1
Chr3 CDS 25949339 25951327 25949339 ID=cds.MsaT012502.1;Parent=MsaT012502.1
Gene Sequence

>MsaT012502.1

ATGGAAGAAGGGTTAGGTCAACACTCAGTTCCTCCCCCGGGTTCCATTGACAAGTATAAGATTCTGGATATTAAGCCTATAAGAAGTTTGATACCTGTTTTTTCAAGGAATCCTCAAGGTCAATCTTCAGGTCAGTATCCTTCTGGGTTTTCTCCATTTTTCCCATTTGGTGGACCTCATGATACTTCAACCGCAAAGCCAAGTAGAGCGATGCCGACTCCACTTCAAGCATTTAGGAGCCCTTTTGGAGAGGATGAGGATCTCAATGACAATGATGGTTTTAGCAACAAAAGAAGTGCGGCAGCATCACATTCAAGCCGGGTCAGATTGAAGAAGCATAAGGTATATAATGATGTTCATGTTGATATGAGTGGTTTAGTTGGTATAAGCCCAGGTCAAAGAGACAATGGTAACCGTGAAGTTGTTAATATTGTGCTCATGACATTTGATGCGCTAAGACGAAGGCTATCTCAACTTGTAGATGCCAAGGAATGGAACACAGGTTCGATCAAACGTACCGATTTAAAAGCTGGCAATATTTGTATGACCAAAGGAATTCGAACAAACACAGCGAAGAGGGTAGGATCAGTACCTGGAGTTGAGATTGGGGACATTTTCTTTTTCCGAATGGAAATGTGTGTTATCGGTTTGCATGCTCAATCGATGGGGGGGATTGATGCCTTGCATATCCAGGGTGACCGCGGGGAAGATACTTTGGCTGTTAGCATTGTTTCGTCAGGAGAATACGACGATGAAGCTGAGGATGGTGATGTTATAATTTATACCGGTCAGGGTGGGAATTTCAACAAGAAAGATAAGCATGTGTCAGATCAGAAGCTTCATAAGGGTAATCTTGCTTTGGATAGAAGTTCACGAACACACAATGAAATAAGAGTCATCCGAGGCATCAAAGATGCTGTGACTCCAGGTGCAAAAATCTATGTCTATGATGGTCTGTATAAAATTCAAGATTCATGGGTTGAAAAAGCAAAAGGAGGCGGTGGCTTGTTTAAGTATAAGTTGATAAGAGTGCCTGGACAACCTAGTGCCTTTGCCGTTTGGAAATCTGTTCAGAAGTGGAAAGCTGGTTTCCCTGCAAGGACTGGACTTATTCTTGCTGACCTCTCCTCGGGAGCCGAGAGTCTTCCAGTATCACTTGTCAATGAAGTCGATAACGTTAAGTCACCTGCCTTTTTCACATATTTCCACTCTCTTAGACATCCGAAATCATTCAGTTTAATGCAGCCTTCACATAGTTGCAGCTGTAGTGGTAAAAAAGCATGTGTCCCTGGCGATTTGGACTGCTCATGCATTCGGAGAAACGAAGGTGATTTTCCTTATATTATCAATGGAGTTCTGGCGAATCGGAAGCCGTTGGTTCATGAATGTGGCCCCACATGTCAGTGTTTTCCTAACTGCAAAAATCGAGTATCCCAAACCGGTTTGAAGCACCAAATGGAAGTGTTCAAAACAAAGGATAAAGGTTGGGGTCTTCGATCATGGGATCCTATTCGTGCTGGTGCTTTTATTTGCGAGTATGCAGGAGAAGTTATCGATAAAGCTAGGTTAAGTCAGCTTGTACAAGAAGGAGATACTGATGAGTATGTTTTCGATACAACCCGTATTTATGAATCTTTCAAGTGGAATTATGAGCCTAAGTTACTAGAAGAAGCGACCACAAACGAGTCTAGTGAGGATTATGCTTTGCCACACCCTCTTATTATAAATGCTAAGAATGTTGGAAATGTGGCTAGATTCATGAATCATAGTTGCTCTCCAAATGTTTTCTGGCAGCCTGTCTTATACGAAGAGAACAACCAATCCTTTCTCCATGTTGCATTTTTTGCATTGAGACATATTCCACCAATGCATGAGTTAACATATGATTATGGTTCTGACCGTTCCAATCATACCGAGGGTAGCAGTGCACGCAAAGGTAGAAAGAAATGCTTATGTGGATCATCAAACTGCCGCGGTTCTTTTACTTGA
Protein sequence

>MsaT012502.1

MEEGLGQHSVPPPGSIDKYKILDIKPIRSLIPVFSRNPQGQSSGQYPSGFSPFFPFGGPHDTSTAKPSRAMPTPLQAFRSPFGEDEDLNDNDGFSNKRSAAASHSSRVRLKKHKVYNDVHVDMSGLVGISPGQRDNGNREVVNIVLMTFDALRRRLSQLVDAKEWNTGSIKRTDLKAGNICMTKGIRTNTAKRVGSVPGVEIGDIFFFRMEMCVIGLHAQSMGGIDALHIQGDRGEDTLAVSIVSSGEYDDEAEDGDVIIYTGQGGNFNKKDKHVSDQKLHKGNLALDRSSRTHNEIRVIRGIKDAVTPGAKIYVYDGLYKIQDSWVEKAKGGGGLFKYKLIRVPGQPSAFAVWKSVQKWKAGFPARTGLILADLSSGAESLPVSLVNEVDNVKSPAFFTYFHSLRHPKSFSLMQPSHSCSCSGKKACVPGDLDCSCIRRNEGDFPYIINGVLANRKPLVHECGPTCQCFPNCKNRVSQTGLKHQMEVFKTKDKGWGLRSWDPIRAGAFICEYAGEVIDKARLSQLVQEGDTDEYVFDTTRIYESFKWNYEPKLLEEATTNESSEDYALPHPLIINAKNVGNVARFMNHSCSPNVFWQPVLYEENNQSFLHVAFFALRHIPPMHELTYDYGSDRSNHTEGSSARKGRKKCLCGSSNCRGSFT*