AlfalfaGEDB | MsaG012766

M. sativa ssp. caerulea / MsaG012766

KEGG: K15032
GO: -
PFAM: PF02536.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012766	XP_004503347.1	84.304	395	25	4	1	358	110	504	0.0	622

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012766	sp\|B6TGN4\|MTEF4_MAIZE	44.010	384	176	4	1	347	103	484	2.87e-108	328

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012766	tr\|A0A072VS35\|A0A072VS35_MEDTR	86.582	395	16	4	1	358	107	501	0.0	634

TFs/TRs:

Gene ID	Type	Classification
MsaG012766	TR	mTERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000070	MsaG012766	0.800457	2.388512e-48	3.262963e-46
MsaG000262	MsaG012766	0.821464	6.998672e-53	1.600048e-50
MsaG000323	MsaG012766	0.828267	1.768772e-54	4.873231e-52
MsaG000690	MsaG012766	0.814596	2.456486e-51	4.700328e-49
MsaG000940	MsaG012766	0.812252	8.001178e-51	1.443776e-48
MsaG001157	MsaG012766	0.822648	3.732347e-53	8.807665e-51
MsaG001726	MsaG012766	0.802016	1.150060e-48	1.627480e-46
MsaG001809	MsaG012766	0.800021	2.927716e-48	3.960408e-46
MsaG001826	MsaG012766	0.800694	2.138342e-48	2.936938e-46
MsaG002727	MsaG012766	0.806471	1.372263e-49	2.153566e-47
MsaG002990	MsaG012766	0.806537	1.329422e-49	2.089505e-47
MsaG003789	MsaG012766	0.823119	2.902830e-53	6.937878e-51
MsaG004307	MsaG012766	0.803771	5.008630e-49	7.380145e-47
MsaG004298	MsaG012766	0.809769	2.744023e-50	4.660018e-48
MsaG004400	MsaG012766	0.831486	2.930850e-55	8.852635e-53
MsaG005035	MsaG012766	0.801539	1.439023e-48	2.014495e-46
MsaG005275	MsaG012766	0.843581	2.395567e-58	1.047980e-55
MsaG005278	MsaG012766	0.810216	2.201377e-50	3.779060e-48
MsaG005623	MsaG012766	0.806425	1.403420e-49	2.200034e-47
MsaG005921	MsaG012766	0.802806	7.918342e-49	1.141003e-46
MsaG006239	MsaG012766	0.812341	7.650287e-51	1.383583e-48
MsaG006309	MsaG012766	0.818762	2.889452e-52	6.151145e-50
MsaG006394	MsaG012766	0.800809	2.026591e-48	2.790602e-46
MsaG006463	MsaG012766	0.830977	3.904652e-55	1.162073e-52
MsaG007099	MsaG012766	0.810466	1.944643e-50	3.359000e-48
MsaG007368	MsaG012766	0.818942	2.631495e-52	5.628611e-50
MsaG007723	MsaG012766	0.804918	2.897174e-49	4.383946e-47
MsaG008004	MsaG012766	0.826920	3.711388e-54	9.846736e-52
MsaG008033	MsaG012766	0.821506	6.844856e-53	1.566648e-50
MsaG008243	MsaG012766	0.800838	1.998831e-48	2.754162e-46
MsaG008331	MsaG012766	0.813930	3.441016e-51	6.475453e-49
MsaG008630	MsaG012766	0.809157	3.708921e-50	6.206125e-48
MsaG009178	MsaG012766	0.801658	1.360576e-48	1.909857e-46
MsaG009739	MsaG012766	0.802426	9.475368e-49	1.353592e-46
MsaG009852	MsaG012766	0.816931	7.452826e-52	1.513350e-49
MsaG010126	MsaG012766	0.832549	1.605782e-55	5.002624e-53
MsaG010429	MsaG012766	0.801059	1.802390e-48	2.495814e-46
MsaG010943	MsaG012766	0.820616	1.095210e-52	2.448230e-50
MsaG011005	MsaG012766	0.803713	5.150875e-49	7.579482e-47
MsaG011213	MsaG012766	0.807246	9.427180e-50	1.506637e-47
MsaG011467	MsaG012766	0.806933	1.097477e-49	1.741020e-47
MsaG011559	MsaG012766	0.819225	2.270435e-52	4.892565e-50
MsaG011658	MsaG012766	0.808362	5.475057e-50	8.987584e-48
MsaG012141	MsaG012766	0.809053	3.901597e-50	6.512595e-48
MsaG012369	MsaG012766	0.803353	6.111033e-49	8.917804e-47
MsaG012610	MsaG012766	0.817242	6.349107e-52	1.299623e-49
MsaG012588	MsaG012766	0.816520	9.204244e-52	1.849261e-49
MsaG012650	MsaG012766	0.801617	1.387607e-48	1.945943e-46
MsaG012766	MsaG013238	0.806087	1.652020e-49	2.568997e-47
MsaG012766	MsaG013274	0.802427	9.472484e-49	1.353192e-46
MsaG012766	MsaG013429	0.810040	2.400478e-50	4.103351e-48
MsaG012766	MsaG013553	0.838738	4.427939e-57	1.661729e-54
MsaG012766	MsaG013828	0.969004	6.992520e-129	6.889535e-123
MsaG012766	MsaG013883	0.808076	6.294386e-50	1.026205e-47
MsaG012766	MsaG014039	0.813018	5.449672e-51	1.002353e-48
MsaG012766	MsaG014094	0.807448	8.548267e-50	1.372780e-47
MsaG012766	MsaG015609	0.828109	1.929324e-54	5.292293e-52
MsaG012766	MsaG015563	0.840404	1.641672e-57	6.490555e-55
MsaG012766	MsaG016107	0.818891	2.701326e-52	5.770155e-50
MsaG012766	MsaG016109	0.803335	6.163294e-49	8.990151e-47
MsaG012766	MsaG016372	0.807588	7.985693e-50	1.286717e-47
MsaG012766	MsaG016887	0.804533	3.483229e-49	5.223884e-47
MsaG012766	MsaG017063	0.837434	9.555543e-57	3.444469e-54
MsaG012766	MsaG017566	0.836238	1.923450e-56	6.686640e-54
MsaG012766	MsaG017713	0.805875	1.829854e-49	2.831314e-47
MsaG012766	MsaG018680	0.829596	8.460257e-55	2.420374e-52
MsaG012766	MsaG018736	0.802757	8.106546e-49	1.166799e-46
MsaG012766	MsaG018790	0.828054	1.989102e-54	5.447684e-52
MsaG012766	MsaG018916	0.821701	6.175144e-53	1.420711e-50
MsaG012766	MsaG019301	0.815673	1.420117e-51	2.792410e-49
MsaG012766	MsaG019719	0.800653	2.180174e-48	2.991572e-46
MsaG012766	MsaG020758	0.807469	8.458688e-50	1.359097e-47
MsaG012766	MsaG021085	0.802142	1.083611e-48	1.537866e-46
MsaG012766	MsaG021320	0.806620	1.276747e-49	2.010722e-47
MsaG012766	MsaG021578	0.815601	1.472931e-51	2.891009e-49
MsaG012766	MsaG021782	0.807381	8.828687e-50	1.415571e-47
MsaG012766	MsaG022245	0.821036	8.775763e-53	1.983835e-50
MsaG012766	MsaG022426	0.814881	2.125575e-51	4.096597e-49
MsaG012766	MsaG022548	0.806692	1.233052e-49	1.945163e-47
MsaG012766	MsaG022579	0.804347	3.806159e-49	5.683816e-47
MsaG012766	MsaG022866	0.812873	5.861300e-51	1.074158e-48
MsaG012766	MsaG023575	0.801472	1.485068e-48	2.075789e-46
MsaG012766	MsaG023602	0.818364	3.554007e-52	7.488000e-50
MsaG012766	MsaG023964	0.819520	1.945653e-52	4.225168e-50
MsaG012766	MsaG024454	0.813259	4.827563e-51	8.932662e-49
MsaG012766	MsaG025330	0.833915	7.362520e-56	2.387492e-53
MsaG012766	MsaG025331	0.802154	1.077617e-48	1.529769e-46
MsaG012766	MsaG026280	0.826856	3.842136e-54	1.017572e-51
MsaG012766	MsaG026465	0.800225	2.661944e-48	3.617376e-46
MsaG012766	MsaG026901	0.818870	2.731069e-52	5.830533e-50
MsaG012766	MsaG027103	0.810154	2.269386e-50	3.889995e-48
MsaG012766	MsaG027610	0.800893	1.947989e-48	2.687401e-46
MsaG012766	MsaG027688	0.800140	2.769856e-48	3.756783e-46
MsaG012766	MsaG028303	0.805997	1.725501e-49	2.677442e-47
MsaG012766	MsaG028419	0.808998	4.009205e-50	6.683154e-48
MsaG012766	MsaG029308	0.810481	1.930892e-50	3.336361e-48
MsaG012766	MsaG029613	0.806728	1.211851e-49	1.913320e-47
MsaG012766	MsaG030046	0.801358	1.567038e-48	2.184678e-46
MsaG012766	MsaG031763	0.813439	4.409139e-51	8.195530e-49
MsaG012766	MsaG032178	0.812800	6.078082e-51	1.111872e-48
MsaG012766	MsaG032340	0.800848	1.989797e-48	2.742289e-46
MsaG012766	MsaG033185	0.805355	2.349178e-49	3.591029e-47
MsaG012766	MsaG033546	0.807965	6.646024e-50	1.080633e-47
MsaG012766	MsaG033851	0.832906	1.310576e-55	4.125817e-53
MsaG012766	MsaG034105	0.800202	2.690123e-48	3.653760e-46
MsaG012766	MsaG034145	0.807294	9.208510e-50	1.473414e-47
MsaG012766	MsaG034625	0.835773	2.519721e-56	8.637672e-54
MsaG012766	MsaG035173	0.811222	1.337006e-50	2.352391e-48
MsaG012766	MsaG035206	0.800754	2.079144e-48	2.859443e-46
MsaG012766	MsaG035867	0.800836	2.000970e-48	2.756980e-46
MsaG012766	MsaG035994	0.801710	1.327976e-48	1.866263e-46
MsaG012766	MsaG038999	0.820070	1.458564e-52	3.214002e-50
MsaG012766	MsaG039607	0.800878	1.962378e-48	2.706322e-46
MsaG012766	MsaG039771	0.803388	6.010527e-49	8.778187e-47
MsaG012766	MsaG039917	0.827102	3.358992e-54	8.957206e-52
MsaG012766	MsaG039878	0.801126	1.746843e-48	2.422620e-46
MsaG012766	MsaG040683	0.804856	2.984190e-49	4.509212e-47
MsaG012766	MsaG043935	0.822456	4.134594e-53	9.706393e-51
MsaG012766	MsaG044119	0.832293	1.856959e-55	5.742161e-53
MsaG012766	MsaG044936	0.812381	7.498721e-51	1.357547e-48
MsaG012766	MsaG045212	0.811079	1.435098e-50	2.516172e-48
MsaG012766	MsaG045601	0.806076	1.660770e-49	2.581950e-47
MsaG012766	MsaG045667	0.805820	1.878340e-49	2.902655e-47
MsaG012766	MsaG045958	0.813595	4.075414e-51	7.605024e-49
MsaG012766	MsaG046196	0.805197	2.534408e-49	3.860013e-47
MsaG012766	MsaG046368	0.806902	1.113713e-49	1.765553e-47
MsaG012766	MsaG046601	0.809672	2.877939e-50	4.875933e-48
MsaG012766	MsaG046831	0.822334	4.411469e-53	1.032292e-50
MsaG012766	MsaG046961	0.816202	1.083512e-51	2.159341e-49
MsaG012766	MsaG047200	0.802307	1.002657e-48	1.428413e-46
MsaG012766	MsaG001347	0.813718	3.830596e-51	7.170047e-49
MsaG012766	MsaG002643	0.803963	4.572178e-49	6.767090e-47
MsaG012766	MsaG004516	0.822041	5.155949e-53	1.197002e-50
MsaG012766	MsaG007436	0.803978	4.538634e-49	6.719777e-47
MsaG012766	MsaG012407	0.829209	1.048996e-54	2.967650e-52
MsaG012766	MsaG014339	0.815075	1.925545e-51	3.729277e-49
MsaG012766	MsaG033256	0.832044	2.138294e-55	6.565112e-53
MsaG012766	MsaG033779	0.818027	4.231939e-52	8.839261e-50
MsaG012766	MsaG036864	0.800639	2.194094e-48	3.009773e-46
MsaG012766	MsaG036708	0.803269	6.358684e-49	9.261176e-47
MsaG012766	MsaG038289	0.812016	8.999801e-51	1.614637e-48
MsaG012766	MsaG035345	0.810542	1.873365e-50	3.241786e-48
MsaG012766	MsaG037636	0.801790	1.278849e-48	1.800518e-46
MsaG012766	MsaG042682	0.806975	1.075444e-49	1.707785e-47
MsaG012766	MsaG041441	0.828144	1.892595e-54	5.196652e-52
MsaG012766	MsaG043786	0.802043	1.135372e-48	1.607719e-46
MsaG012766	MsaG044505	0.802604	8.713533e-49	1.249840e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012766	MtrunA17_Chr1g0212691	86.582	395	16	4	1	358	107	501	0.0	634
MsaG012766	MtrunA17_Chr8g0391881	70.093	321	28	4	1	284	85	374	1.76e-141	406
MsaG012766	MtrunA17_Chr5g0428941	47.253	364	153	4	1	325	139	502	1.21e-107	325
MsaG012766	MtrunA17_Chr8g0391861	85.714	112	14	1	247	358	1	110	1.37e-52	170
MsaG012766	MtrunA17_Chr8g0391871	88.333	60	7	0	299	358	1	60	1.58e-29	108
MsaG012766	MtrunA17_Chr2g0294671	27.273	319	183	11	1	312	211	487	1.23e-24	104
MsaG012766	MtrunA17_Chr4g0034981	24.116	311	198	4	1	307	309	585	2.12e-23	101
MsaG012766	MtrunA17_Chr4g0043941	23.986	296	182	7	18	308	62	319	4.51e-23	98.2
MsaG012766	MtrunA17_Chr6g0448821	24.845	322	191	7	5	313	79	362	7.34e-22	95.5
MsaG012766	MtrunA17_Chr5g0445491	26.038	265	181	7	34	289	192	450	7.28e-17	81.6
MsaG012766	MtrunA17_Chr2g0311181	23.490	298	178	7	39	332	75	326	2.02e-16	79.3
MsaG012766	MtrunA17_Chr5g0417641	30.332	211	136	5	104	308	88	293	3.45e-15	75.5
MsaG012766	MtrunA17_Chr2g0304101	24.444	270	132	5	79	312	76	309	8.41e-15	74.3
MsaG012766	MtrunA17_Chr3g0127891	23.453	307	191	10	5	307	225	491	2.97e-14	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG012766	AT2G44020.1	70.707	396	78	4	1	358	112	507	0.0	540
MsaG012766	AT4G02990.2	47.207	358	152	3	1	321	147	504	1.25e-107	326
MsaG012766	AT4G02990.1	47.207	358	152	3	1	321	147	504	1.25e-107	326
MsaG012766	AT2G21710.1	23.276	348	225	5	1	343	316	626	1.41e-26	111
MsaG012766	AT4G38160.4	23.667	300	185	6	18	311	63	324	2.04e-25	105
MsaG012766	AT4G38160.1	23.667	300	185	6	18	311	63	324	2.04e-25	105
MsaG012766	AT4G38160.2	23.667	300	185	6	18	311	63	324	2.78e-25	105
MsaG012766	AT4G38160.3	23.667	300	185	6	18	311	63	324	5.93e-25	104
MsaG012766	AT1G78930.1	24.299	321	231	8	1	313	264	580	4.45e-23	100
MsaG012766	AT4G14605.2	25.632	277	161	6	35	308	234	468	2.95e-20	92.4
MsaG012766	AT4G14605.1	25.632	277	161	6	35	308	234	468	2.95e-20	92.4
MsaG012766	AT5G54180.1	27.444	266	173	9	54	309	235	490	9.45e-18	84.7
MsaG012766	AT2G21710.2	21.739	276	176	4	1	271	316	556	1.17e-14	75.5
MsaG012766	AT5G55580.2	23.047	256	155	6	37	287	117	335	1.58e-14	74.7
MsaG012766	AT1G61980.3	22.917	336	207	6	30	319	81	410	1.83e-14	74.7
MsaG012766	AT1G61980.2	22.917	336	207	6	30	319	81	410	1.83e-14	74.7
MsaG012766	AT1G61980.1	22.917	336	207	6	30	319	81	410	1.83e-14	74.7
MsaG012766	AT5G55580.1	23.047	256	155	6	37	287	223	441	5.06e-14	73.6
MsaG012766	AT2G21710.3	21.533	274	175	4	1	269	316	554	8.45e-14	72.8
MsaG012766	AT2G03050.1	26.378	254	147	7	61	311	56	272	6.56e-13	68.9
MsaG012766	AT2G03050.2	26.378	254	147	7	61	311	57	273	7.99e-13	68.6
MsaG012766	AT2G34620.1	31.532	111	75	1	204	313	186	296	2.44e-11	64.3

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAGTATCCTTTGATTT+TGG	0.075546	3:+32161497	MsaT012766.1:CDS
CTCCTGTTATTAAGTTTCTT+AGG	0.190808	3:+32161652	MsaT012766.1:CDS
GTTGAGTTTATTCAGAAATT+AGG	0.214945	3:+32161456	MsaT012766.1:CDS
TCAGTATCCACAGATTATAT+TGG	0.279500	3:+32161988	MsaT012766.1:CDS
AGAATACTTCACCTATAGTT+TGG	0.284806	3:+32162311	MsaT012766.1:CDS
AAACTATAGGTGAAGTATTC+TGG	0.305579	3:-32162309	None:intergenic
TCACCTATAGTTTGGAATCT+AGG	0.308236	3:+32162319	MsaT012766.1:CDS
CTCTTAAGCCTCTGGTATCT+AGG	0.309752	3:-32162348	None:intergenic
GGATAAAGTGTTCATTGAAT+TGG	0.316271	3:+32162376	MsaT012766.1:CDS
TCCTGTTATTAAGTTTCTTA+GGG	0.320182	3:+32161653	MsaT012766.1:CDS
TTATATTGGGTTGCCTCTTA+AGG	0.346882	3:+32162002	MsaT012766.1:CDS
AGCTTAAACCCTAAAAGTTC+AGG	0.348375	3:-32161723	None:intergenic
CCCCTAAGAAACTTAATAAC+AGG	0.351787	3:-32161654	None:intergenic
ATGTAGAGAAAGATGATATT+GGG	0.358260	3:+32161682	MsaT012766.1:CDS
GAAGACAATTTAGCCTTAAG+AGG	0.360188	3:-32162015	None:intergenic
CAGTATCCACAGATTATATT+GGG	0.362497	3:+32161989	MsaT012766.1:CDS
AAAGCCTCTTGTTGATTACT+TGG	0.364443	3:+32161866	MsaT012766.1:CDS
TCAAAATGTGATTATGAAAC+CGG	0.364444	3:+32162128	MsaT012766.1:CDS
ATCTGAAACTATATCATTCC+CGG	0.367449	3:-32162505	None:intergenic
TTAGTATTGGTGTTAATCCT+AGG	0.373470	3:+32161781	MsaT012766.1:CDS
GATGTAGAGAAAGATGATAT+TGG	0.374971	3:+32161681	MsaT012766.1:CDS
TCCCCTTGCTCTTAAGCCTC+TGG	0.375995	3:-32162356	None:intergenic
ATCGCAGGATGTGGCTAGTA+TGG	0.382352	3:+32162179	MsaT012766.1:CDS
GTGGCTTATCTTGTTAGTAT+TGG	0.382878	3:+32161768	MsaT012766.1:CDS
GAGATTTGAAGAGAGATTGC+AGG	0.401297	3:+32162419	MsaT012766.1:CDS
TCCTAGGGATATTGGTCCTA+TGG	0.421118	3:+32161797	MsaT012766.1:CDS
GCAATCTCTCTTCAAATCTC+TGG	0.437449	3:-32162416	None:intergenic
AATCCTAGATTCCAAACTAT+AGG	0.441030	3:-32162322	None:intergenic
GAATGAAGGACCTATACTTT+CGG	0.441319	3:-32162457	None:intergenic
ACCACCCTTGCAAACCCTTC+CGG	0.446986	3:-32162075	None:intergenic
GGTGTTAATCCTAGGGATAT+TGG	0.451217	3:+32161789	MsaT012766.1:CDS
ATAGGTCCTTCATTCTGTAT+TGG	0.452739	3:+32162465	MsaT012766.1:CDS
TATAGTTTCAGATGAGGAAG+AGG	0.454451	3:+32162515	MsaT012766.1:CDS
TACTTCTTCAAAAGTGAGAT+GGG	0.459036	3:+32162258	MsaT012766.1:CDS
GAGGAAGGAATGTTTGCCTT+CGG	0.461475	3:+32161958	MsaT012766.1:CDS
TCCGGAAGGGTTTGCAAGGG+TGG	0.482240	3:+32162074	MsaT012766.1:CDS
ATACTGATTTATATCATCAA+TGG	0.482461	3:-32161484	None:intergenic
GGGATTTCTAGGTCTAAACT+TGG	0.492563	3:+32161573	MsaT012766.1:CDS
GGTCCTTCATTCTGTATTGG+TGG	0.495994	3:+32162468	MsaT012766.1:CDS
GGAAGGGTTTGCAAGGGTGG+TGG	0.497371	3:+32162077	MsaT012766.1:CDS
TACCAGAGGCTTAAGAGCAA+GGG	0.502139	3:+32162354	MsaT012766.1:CDS
GGTGATCAAGTGTCCTCAAT+TGG	0.503619	3:+32162200	MsaT012766.1:CDS
TTACTTCTTCAAAAGTGAGA+TGG	0.508844	3:+32162257	MsaT012766.1:CDS
TTTGAAGCTGAAGATTGATC+CGG	0.520863	3:+32162056	MsaT012766.1:CDS
TAGTATTGGTGTTAATCCTA+GGG	0.522188	3:+32161782	MsaT012766.1:CDS
ACTCGCAGCGCAACCAATTG+AGG	0.528224	3:-32162213	None:intergenic
TATATCGAAACCGAAAGTAT+AGG	0.540273	3:+32162447	MsaT012766.1:CDS
GTCCTTCATTCTGTATTGGT+GGG	0.540317	3:+32162469	MsaT012766.1:CDS
GGCAATACCATCGCAGGATG+TGG	0.548475	3:+32162170	MsaT012766.1:CDS
AGCTGAAGATTGATCCGGAA+GGG	0.553058	3:+32162061	MsaT012766.1:CDS
TGATGAAGACTAACCACTTG+TGG	0.559927	3:-32162108	None:intergenic
AGATTTGAAGAGAGATTGCA+GGG	0.561148	3:+32162420	MsaT012766.1:CDS
ATACCAGAGGCTTAAGAGCA+AGG	0.565715	3:+32162353	MsaT012766.1:CDS
TTCCCACCAATACAGAATGA+AGG	0.566084	3:-32162471	None:intergenic
TTGATCCGGAAGGGTTTGCA+AGG	0.567631	3:+32162070	MsaT012766.1:CDS
CCTGTTATTAAGTTTCTTAG+GGG	0.577517	3:+32161654	MsaT012766.1:CDS
ACACTAGCATGCAAAACTTG+TGG	0.577739	3:-32161615	None:intergenic
GAGACCTGTAAAAGAGCTTG+TGG	0.581965	3:+32162281	MsaT012766.1:CDS
TAAGGATACTGTGTAACCAT+AGG	0.587849	3:-32161813	None:intergenic
ACACTACAACCCAAAATCAA+AGG	0.594517	3:-32161507	None:intergenic
AGGCAACCCAATATAATCTG+TGG	0.598668	3:-32161995	None:intergenic
ACCAGAGGCTTAAGAGCAAG+GGG	0.605082	3:+32162355	MsaT012766.1:CDS
ATAACCAAGTAATCAACAAG+AGG	0.605793	3:-32161870	None:intergenic
TGGTAGGGCAATACCATCGC+AGG	0.609868	3:+32162164	MsaT012766.1:CDS
TCTGAAACTATATCATTCCC+GGG	0.612098	3:-32162504	None:intergenic
GTGGGAAATTAGAGCTACCC+GGG	0.615038	3:+32162487	MsaT012766.1:CDS
GGTGGGAAATTAGAGCTACC+CGG	0.623613	3:+32162486	MsaT012766.1:CDS
AGGTACTATGAGTACTTCAG+TGG	0.624393	3:+32161749	MsaT012766.1:CDS
ACCATAGGACCAATATCCCT+AGG	0.641670	3:-32161798	None:intergenic
ACTTCTTCAAAAGTGAGATG+GGG	0.645282	3:+32162259	MsaT012766.1:CDS
TGATCCGGAAGGGTTTGCAA+GGG	0.654444	3:+32162071	MsaT012766.1:CDS
GAATGATATAGTTTCAGATG+AGG	0.656227	3:+32162509	MsaT012766.1:CDS
ATACTAGCCACATCCTGCGA+TGG	0.661576	3:-32162177	None:intergenic
AAGCTGAAGATTGATCCGGA+AGG	0.664980	3:+32162060	MsaT012766.1:CDS
GGTGGAGAAGATGCCACAAG+TGG	0.668174	3:+32162095	MsaT012766.1:CDS
GGATACTGAGCAATAACCGA+AGG	0.679020	3:-32161974	None:intergenic
GGATTAAACCTAGATACCAG+AGG	0.689589	3:+32162340	MsaT012766.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	32161444	32162581	32161444	ID=MsaG012766
Chr3	mRNA	32161444	32162581	32161444	ID=MsaT012766.1;Parent=MsaG012766
Chr3	exon	32161444	32161887	32161444	ID=MsaT012766.1.exon1;Parent=MsaT012766.1
Chr3	CDS	32161444	32161887	32161444	ID=cds.MsaT012766.1;Parent=MsaT012766.1
Chr3	exon	32161949	32162581	32161949	ID=MsaT012766.1.exon2;Parent=MsaT012766.1
Chr3	CDS	32161949	32162581	32161949	ID=cds.MsaT012766.1;Parent=MsaT012766.1

Gene Sequence

>MsaT012766.1

ATGAGGGAACGTGTTGAGTTTATTCAGAAATTAGGTTTAACCATTGATGATATAAATCAGTATCCTTTGATTTTGGGTTGTAGTGTTAGAAAAAACATGATTCCTGTTTTAGGTTATTTAGAAAAAATTGGGATTTCTAGGTCTAAACTTGGTGAATTTATTAAGAATTATCCACAAGTTTTGCATGCTAGTGTTATTGTTGAGCTTGCTCCTGTTATTAAGTTTCTTAGGGGACTTGATGTAGAGAAAGATGATATTGGGTTTGTGTTGCAAAAATATCCTGAACTTTTAGGGTTTAAGCTTGAAGGTACTATGAGTACTTCAGTGGCTTATCTTGTTAGTATTGGTGTTAATCCTAGGGATATTGGTCCTATGGTTACACAGTATCCTTATTTTTTAGGGATGAGAGTTGGTACTATGATAAAGCCTCTTGTTGATTACTTGTTTTGGTTTGAGGAAGGAATGTTTGCCTTCGGTTATTGCTCAGTATCCACAGATTATATTGGGTTGCCTCTTAAGGCTAAATTGTCTTCACAACAAAAAAATTTCAGTTTGAAGCTGAAGATTGATCCGGAAGGGTTTGCAAGGGTGGTGGAGAAGATGCCACAAGTGGTTAGTCTTCATCAAAATGTGATTATGAAACCGGTTGAGTTTTTACTTGGTAGGGCAATACCATCGCAGGATGTGGCTAGTATGGTGATCAAGTGTCCTCAATTGGTTGCGCTGCGAGTTGAGCTCATGAAGAACAGTTATTACTTCTTCAAAAGTGAGATGGGGAGACCTGTAAAAGAGCTTGTGGAGTTTCCAGAATACTTCACCTATAGTTTGGAATCTAGGATTAAACCTAGATACCAGAGGCTTAAGAGCAAGGGGATAAAGTGTTCATTGAATTGGATGCTCAATTGTAGTGACCAGAGATTTGAAGAGAGATTGCAGGGCAATTATATCGAAACCGAAAGTATAGGTCCTTCATTCTGTATTGGTGGGAAATTAGAGCTACCCGGGAATGATATAGTTTCAGATGAGGAAGAGGAGAGTGATGATGAAATGCTAAACAGAAGAACTGTTTCTCTTTAG

Protein sequence

>MsaT012766.1

MRERVEFIQKLGLTIDDINQYPLILGCSVRKNMIPVLGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLDVEKDDIGFVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVDYLFWFEEGMFAFGYCSVSTDYIGLPLKAKLSSQQKNFSLKLKIDPEGFARVVEKMPQVVSLHQNVIMKPVEFLLGRAIPSQDVASMVIKCPQLVALRVELMKNSYYFFKSEMGRPVKELVEFPEYFTYSLESRIKPRYQRLKSKGIKCSLNWMLNCSDQRFEERLQGNYIETESIGPSFCIGGKLELPGNDIVSDEEEESDDEMLNRRTVSL*