AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012970 MtrunA17_Chr3g0090531 97.326 187 5 0 1 187 1 187 6.13e-135 375
MsaG012970 MtrunA17_Chr4g0036971 44.828 203 86 5 1 183 1 197 7.89e-52 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012970 AT3G51960.3 56.250 128 50 1 41 162 10 137 1.41e-47 154
MsaG012970 AT3G51960.2 49.405 168 75 4 4 162 10 176 1.95e-47 155
MsaG012970 AT3G51960.1 49.701 167 75 4 4 162 10 175 2.43e-47 154
MsaG012970 AT2G16770.2 55.882 136 47 5 46 169 35 169 5.72e-46 152
MsaG012970 AT2G16770.1 55.882 136 47 5 46 169 35 169 5.72e-46 152
MsaG012970 AT4G35040.1 46.703 182 75 6 1 165 1 177 4.05e-45 150
MsaG012970 AT4G35040.4 46.703 182 75 6 1 165 1 177 4.05e-45 150
MsaG012970 AT4G35040.2 46.703 182 75 6 1 165 1 177 4.05e-45 150
MsaG012970 AT4G35040.3 46.703 182 75 6 1 165 1 177 4.05e-45 150

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGCAGAAACAGCTTATT+TGG 0.185081 3:+37776687 MsaT012970.1:CDS
TGAAAATTGCTACATGATTC+TGG 0.223560 3:-37776442 None:intergenic
GTCAAGGTTAAGGAACATTT+TGG 0.239454 3:+37776792 MsaT012970.1:CDS
GGAAAAGTTGACAATGAATT+AGG 0.247864 3:+37776826 MsaT012970.1:CDS
AAAGTATAGGGAGAAGAAAA+AGG 0.319200 3:+37776669 MsaT012970.1:CDS
AAGCTGAATTGTCAAGGTTA+AGG 0.395629 3:+37776782 MsaT012970.1:CDS
ACACAAATTCAAGGCCAAAA+AGG 0.410549 3:+37776620 MsaT012970.1:CDS
ACATGCAGGATCATCATCTA+AGG 0.417339 3:+37776888 MsaT012970.1:CDS
GTAGTTTCCTTAAAAGCTGT+TGG 0.444956 3:-37776741 None:intergenic
AAGAAAGAAAATATGGAAGA+TGG 0.479572 3:+37776370 None:intergenic
CTGAAGATGACACAAATTCA+AGG 0.502147 3:+37776611 MsaT012970.1:CDS
AACAGCTTATTTGGAAGAAG+AGG 0.515408 3:+37776696 MsaT012970.1:CDS
GTCCTTTAGTACTACTCATG+TGG 0.520570 3:-37776934 None:intergenic
CCATGTTTGCATCCACATGC+AGG 0.525489 3:+37776874 MsaT012970.1:CDS
TTTAAGGAAACTACAAGGGC+AGG 0.534031 3:+37776750 MsaT012970.1:CDS
CCTGCATGTGGATGCAAACA+TGG 0.553344 3:-37776874 None:intergenic
TGTGTGTGATAAGCATCATC+AGG 0.565504 3:-37776553 None:intergenic
TACTTGAAGCTGAATTGTCA+AGG 0.575730 3:+37776776 MsaT012970.1:CDS
TGATAAGCATCATCAGGACC+AGG 0.614933 3:-37776547 None:intergenic
TTAGATGATGATCCTGCATG+TGG 0.661059 3:-37776886 None:intergenic
ACTCACACTTGTAATCCACC+TGG 0.661329 3:+37776529 MsaT012970.1:CDS
TAAGCATCATCAGGACCAGG+TGG 0.696090 3:-37776544 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 37776382 37776945 37776382 ID=MsaG012970
Chr3 mRNA 37776382 37776945 37776382 ID=MsaT012970.1;Parent=MsaG012970
Chr3 exon 37776382 37776945 37776382 ID=MsaT012970.1.exon1;Parent=MsaT012970.1
Chr3 CDS 37776382 37776945 37776382 ID=cds.MsaT012970.1;Parent=MsaT012970.1
Gene Sequence

>MsaT012970.1

ATGGAAGATGGAAATTCTGAAGTTTCTGATAAACTTTCAGATAATGTTCTATTTTCAAATCCAGAATCATGTAGCAATTTTCAAACTTCAACATCTTTGAACACAGTTACTGAATTGTCTAATAGCACCACAAAAACATGCATTCACACTCACACTTGTAATCCACCTGGTCCTGATGATGCTTATCACACACACACATGTTTTCACACACATACTCAAGTTTTTGCATCTGAAGATGACACAAATTCAAGGCCAAAAAGGACTTCTGGCAATAGAGAAGCTGTTAAAAAGTATAGGGAGAAGAAAAAGGCAGAAACAGCTTATTTGGAAGAAGAGGTTAAGAAATTGAAACTTGTGAACCAACAGCTTTTAAGGAAACTACAAGGGCAGGCACTACTTGAAGCTGAATTGTCAAGGTTAAGGAACATTTTGGTACAGCTTAAGGGAAAAGTTGACAATGAATTAGGTTCTTTTCCTTTTGAGAAAAATCTGCCATGTTTGCATCCACATGCAGGATCATCATCTAAGGTTAACACTTGTGCATATTGCAAACCACATGAGTAG
Protein sequence

>MsaT012970.1

MEDGNSEVSDKLSDNVLFSNPESCSNFQTSTSLNTVTELSNSTTKTCIHTHTCNPPGPDDAYHTHTCFHTHTQVFASEDDTNSRPKRTSGNREAVKKYREKKKAETAYLEEEVKKLKLVNQQLLRKLQGQALLEAELSRLRNILVQLKGKVDNELGSFPFEKNLPCLHPHAGSSSKVNTCAYCKPHE*