AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG012993


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012993 MtrunA17_Chr2g0291021 80.892 157 8 1 1 135 1 157 9.85e-74 224
MsaG012993 MtrunA17_Chr7g0254981 60.000 110 21 1 49 135 50 159 1.61e-36 127
MsaG012993 MtrunA17_Chr7g0261911 52.846 123 32 2 33 135 252 368 4.72e-32 119
MsaG012993 MtrunA17_Chr1g0175091 47.059 119 45 1 35 135 244 362 3.41e-29 111
MsaG012993 MtrunA17_Chr1g0190301 50.000 126 40 2 33 135 156 281 4.72e-29 110
MsaG012993 MtrunA17_Chr8g0356501 53.636 110 28 2 49 135 37 146 8.15e-28 106
MsaG012993 MtrunA17_Chr7g0274471 51.852 108 32 1 48 135 217 324 1.52e-27 107
MsaG012993 MtrunA17_Chr2g0310011 52.727 110 29 2 49 135 35 144 1.82e-26 102
MsaG012993 MtrunA17_Chr7g0258551 40.650 123 54 1 44 147 207 329 6.59e-26 102
MsaG012993 MtrunA17_Chr5g0409731 47.414 116 29 2 52 135 28 143 1.15e-25 99.8
MsaG012993 MtrunA17_Chr4g0042451 43.333 120 37 3 47 135 15 134 5.36e-25 98.6
MsaG012993 MtrunA17_Chr2g0290991 42.857 119 36 1 49 135 39 157 4.90e-23 93.2
MsaG012993 MtrunA17_Chr6g0461641 43.200 125 35 3 47 135 40 164 6.13e-23 94.0
MsaG012993 MtrunA17_Chr5g0417521 38.346 133 50 2 47 147 13 145 2.64e-22 91.7
MsaG012993 MtrunA17_Chr1g0197171 42.857 112 35 2 49 131 44 155 7.43e-22 89.7
MsaG012993 MtrunA17_Chr7g0242521 43.119 109 40 2 49 135 13 121 2.39e-20 84.7
MsaG012993 MtrunA17_Chr2g0321461 38.760 129 39 2 47 135 43 171 2.63e-20 86.7
MsaG012993 MtrunA17_Chr1g0175071 38.686 137 65 2 49 166 177 313 1.55e-19 83.6
MsaG012993 MtrunA17_Chr2g0288601 68.627 51 16 0 52 102 74 124 5.08e-17 73.2
MsaG012993 MtrunA17_Chr1g0175081 44.615 65 36 0 35 99 8 72 1.40e-15 68.6
MsaG012993 MtrunA17_Chr7g0243081 63.265 49 18 0 52 100 74 122 8.85e-15 70.9
MsaG012993 MtrunA17_Chr2g0283951 62.264 53 20 0 52 104 92 144 3.40e-14 69.3
MsaG012993 MtrunA17_Chr3g0095611 54.717 53 24 0 52 104 22 74 4.38e-14 67.4
MsaG012993 MtrunA17_Chr6g0479431 63.265 49 18 0 52 100 205 253 4.87e-14 68.9
MsaG012993 MtrunA17_Chr1g0162931 62.264 53 20 0 52 104 21 73 4.96e-14 68.2
MsaG012993 MtrunA17_Chr1g0210911 50.725 69 34 0 36 104 9 77 7.00e-14 68.2
MsaG012993 MtrunA17_Chr2g0320231 50.725 69 34 0 32 100 80 148 7.89e-14 68.2
MsaG012993 MtrunA17_Chr8g0379121 63.265 49 18 0 52 100 114 162 1.02e-13 68.2
MsaG012993 MtrunA17_Chr8g0366281 63.265 49 18 0 52 100 176 224 1.32e-13 67.4
MsaG012993 MtrunA17_Chr7g0258531 32.000 150 75 3 13 135 171 320 1.87e-13 67.0
MsaG012993 MtrunA17_Chr5g0410741 63.265 49 18 0 52 100 222 270 1.99e-13 67.0
MsaG012993 MtrunA17_Chr3g0109981 63.265 49 18 0 52 100 277 325 2.80e-13 67.0
MsaG012993 MtrunA17_Chr1g0198601 38.889 108 54 3 2 99 156 261 8.73e-13 65.1
MsaG012993 MtrunA17_Chr1g0154261 61.224 49 19 0 52 100 129 177 9.13e-13 64.7
MsaG012993 MtrunA17_Chr5g0402631 42.593 108 55 3 49 155 214 315 4.56e-12 63.2
MsaG012993 MtrunA17_Chr4g0009991 47.761 67 34 1 41 107 59 124 1.40e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG012993 AT2G01060.1 74.561 114 8 1 43 135 9 122 1.79e-48 159
MsaG012993 AT5G29000.1 51.538 130 38 3 29 135 170 297 6.35e-30 112
MsaG012993 AT5G29000.3 51.538 130 38 3 29 135 170 297 6.35e-30 112
MsaG012993 AT4G13640.2 50.862 116 25 3 52 135 40 155 8.47e-30 110
MsaG012993 AT4G13640.1 50.862 116 25 3 52 135 40 155 8.88e-30 110
MsaG012993 AT5G29000.2 51.538 130 38 3 29 135 213 340 1.02e-29 112
MsaG012993 AT4G28610.1 50.000 126 40 2 31 135 209 332 1.71e-29 112
MsaG012993 AT1G79430.2 53.636 110 28 1 49 135 34 143 2.84e-29 110
MsaG012993 AT3G24120.1 50.862 116 25 3 52 135 44 159 4.64e-29 108
MsaG012993 AT3G24120.2 50.862 116 25 3 52 135 44 159 5.28e-29 108
MsaG012993 AT3G04450.4 46.923 130 42 3 30 135 181 307 9.48e-28 107
MsaG012993 AT3G04450.2 46.923 130 42 3 30 135 181 307 9.48e-28 107
MsaG012993 AT3G04450.1 46.923 130 42 3 30 135 221 347 1.29e-27 107
MsaG012993 AT5G06800.1 46.610 118 42 2 37 135 182 297 2.22e-27 105
MsaG012993 AT5G06800.2 44.715 123 42 2 37 135 182 302 1.22e-26 103
MsaG012993 AT2G20400.1 50.467 107 33 1 49 135 231 337 1.19e-25 101
MsaG012993 AT2G20400.2 50.000 108 33 1 49 135 231 338 1.50e-25 101
MsaG012993 AT3G13040.3 46.154 117 38 2 44 135 236 352 9.16e-25 99.8
MsaG012993 AT3G13040.1 46.154 117 38 2 44 135 236 352 9.16e-25 99.8
MsaG012993 AT3G13040.2 46.154 117 38 2 44 135 236 352 9.16e-25 99.8
MsaG012993 AT1G69580.1 42.063 126 36 3 47 135 32 157 8.73e-24 95.9
MsaG012993 AT1G69580.2 41.732 127 36 3 47 135 32 158 1.27e-23 95.5
MsaG012993 AT3G04030.1 41.060 151 48 5 23 135 19 166 2.16e-22 92.8
MsaG012993 AT3G12730.1 45.045 111 33 3 52 134 26 136 2.18e-22 90.1
MsaG012993 AT3G04030.3 41.060 151 48 5 23 135 19 166 2.61e-22 92.4
MsaG012993 AT5G18240.2 42.742 124 36 3 47 135 43 166 3.96e-22 92.0
MsaG012993 AT5G18240.3 42.742 124 36 3 47 135 43 166 3.96e-22 92.0
MsaG012993 AT5G18240.5 39.310 145 50 4 29 135 22 166 4.74e-22 91.7
MsaG012993 AT5G18240.1 39.310 145 50 4 29 135 22 166 4.81e-22 91.7
MsaG012993 AT5G18240.4 39.310 145 50 4 29 135 22 166 4.81e-22 91.7
MsaG012993 AT5G45580.1 72.917 48 13 0 52 99 10 57 3.97e-20 84.7
MsaG012993 AT5G29000.4 61.972 71 25 1 29 99 213 281 4.19e-20 85.5
MsaG012993 AT5G45580.2 72.917 48 13 0 52 99 26 73 5.59e-20 84.7
MsaG012993 AT2G01060.2 65.753 73 4 1 84 135 1 73 5.68e-20 84.0
MsaG012993 AT3G04030.2 40.397 151 48 6 23 135 19 165 8.87e-20 85.5
MsaG012993 AT3G04450.3 64.286 56 20 0 44 99 233 288 8.10e-19 82.0
MsaG012993 AT5G06800.3 55.556 63 26 1 37 99 182 242 6.50e-18 79.3
MsaG012993 AT4G04605.1 64.151 53 19 0 52 104 17 69 1.05e-15 72.4
MsaG012993 AT2G06020.1 42.308 78 42 1 25 99 59 136 2.40e-15 72.4
MsaG012993 AT2G40260.1 69.388 49 15 0 52 100 85 133 2.88e-15 72.8
MsaG012993 AT2G42660.1 67.347 49 16 0 52 100 53 101 2.92e-15 71.6
MsaG012993 AT5G42630.1 34.783 115 70 1 52 166 108 217 4.71e-15 71.2
MsaG012993 AT5G42630.2 39.394 99 55 1 52 150 108 201 2.98e-14 68.6
MsaG012993 AT2G38300.1 63.265 49 18 0 52 100 57 105 1.02e-13 68.2
MsaG012993 AT1G14600.1 67.347 49 16 0 52 100 26 74 1.19e-13 67.4
MsaG012993 AT2G02060.2 67.347 49 16 0 52 100 32 80 1.58e-13 67.0
MsaG012993 AT2G02060.1 67.347 49 16 0 52 100 32 80 1.58e-13 67.0
MsaG012993 AT1G32240.2 39.796 98 46 1 52 136 216 313 1.98e-13 67.4
MsaG012993 AT1G32240.1 39.796 98 46 1 52 136 216 313 2.06e-13 67.4
MsaG012993 AT4G17695.1 57.143 56 24 0 52 107 167 222 2.48e-13 67.0
MsaG012993 AT5G16560.1 63.265 49 18 0 52 100 222 270 2.84e-13 67.0
MsaG012993 AT4G37180.3 55.357 56 25 0 45 100 206 261 1.45e-12 64.3
MsaG012993 AT3G25790.2 59.574 47 19 0 54 100 201 247 3.65e-12 63.2
MsaG012993 AT4G37180.1 54.545 55 25 0 45 99 206 260 6.09e-12 63.2
MsaG012993 AT4G37180.2 54.545 55 25 0 45 99 213 267 7.44e-12 62.8
MsaG012993 AT4G04580.1 53.061 49 23 0 52 100 17 65 7.85e-12 60.8
MsaG012993 AT1G49560.2 59.615 52 21 0 49 100 192 243 4.35e-11 60.1
MsaG012993 AT3G25790.1 58.696 46 19 0 54 99 201 246 5.15e-11 60.5

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGCAATCCATTAGTTAA+TGG 0.290398 3:-38628546 MsaT012993.1:CDS
ATGGCTCTTAACATGGTAAA+TGG 0.306660 3:+38628120 None:intergenic
CCATGTTAAGAGCCATTTGC+AGG 0.349218 3:-38628113 MsaT012993.1:CDS
ATATGTTGACCATTAACTAA+TGG 0.354268 3:+38628537 None:intergenic
GATATGCTTTCCATTCTAGA+TGG 0.374580 3:-38627779 MsaT012993.1:CDS
CATAACTCTGAGGACACCTT+TGG 0.375653 3:+38628159 None:intergenic
CAAAGGTGTCCTCAGAGTTA+TGG 0.377183 3:-38628158 MsaT012993.1:CDS
AATGGTCAACATATAGATTC+TGG 0.392543 3:-38628528 MsaT012993.1:CDS
AAAGGTGTCCTCAGAGTTAT+GGG 0.402741 3:-38628157 MsaT012993.1:CDS
TTTGTTGAGAGTGGTGATCT+TGG 0.403843 3:+38626874 None:intergenic
TGAGTTGAGAATAGAGTTGT+TGG 0.413671 3:+38628464 None:intergenic
GTTGCTGATTCGAGTTTAGT+TGG 0.426457 3:-38628567 MsaT012993.1:CDS
AGTGGTGATCTTGGTGATGA+TGG 0.462542 3:+38626883 None:intergenic
GTTGATGCTGTGGCTCAACT+TGG 0.466308 3:-38628399 MsaT012993.1:CDS
GAAGCGACTACATGAACAAT+TGG 0.469698 3:-38627403 MsaT012993.1:CDS
ATAACTCTGAGGACACCTTT+GGG 0.476739 3:+38628160 None:intergenic
GGTAGAAGCGCAATGGATCC+TGG 0.480877 3:-38628507 MsaT012993.1:CDS
CTCAGAGTTATGGGTGTACA+AGG 0.485843 3:-38628148 MsaT012993.1:CDS
TGAAGCACTAAAGCTTCAAA+TGG 0.488754 3:-38627433 MsaT012993.1:CDS
TCACGATGAACCATCTAGAA+TGG 0.492563 3:+38627769 None:intergenic
AAGTGCACATTACCTGCAAA+TGG 0.502884 3:+38628101 None:intergenic
AGCGCAATGGATCCTGGTAG+TGG 0.503101 3:-38628501 MsaT012993.1:CDS
CCCTTAGGTGCCACACCCAA+AGG 0.505122 3:-38628175 MsaT012993.1:intron
TAAAAGACGTGTCTAGGAGT+AGG 0.511340 3:+38626807 None:intergenic
GATGCTGTGGCTCAACTTGG+TGG 0.512420 3:-38628396 MsaT012993.1:CDS
CTCGAATCAGCAACATTCGT+TGG 0.516899 3:+38628576 None:intergenic
CCTGCAAATGGCTCTTAACA+TGG 0.525821 3:+38628113 None:intergenic
ACTCAAAGCAACTCTTGCGT+TGG 0.536955 3:-38628446 MsaT012993.1:CDS
AAAAGACGTGTCTAGGAGTA+GGG 0.546153 3:+38626808 None:intergenic
TCTGAGGACACCTTTGGGTG+TGG 0.554653 3:+38628165 None:intergenic
TATACTCAGGATAGTGAACC+AGG 0.561334 3:-38626928 MsaT012993.1:intron
AGACTGTTTCCTCCACTACC+AGG 0.568775 3:+38628489 None:intergenic
GGTTGGTCGTCAAAGACTAA+AGG 0.576564 3:+38626904 None:intergenic
GTGATCTTGGTGATGATGGT+TGG 0.593758 3:+38626887 None:intergenic
GCGACTACATGAACAATTGG+AGG 0.598747 3:-38627400 MsaT012993.1:intron
TGAACGCTTTGTTGATGCTG+TGG 0.601507 3:-38628409 MsaT012993.1:CDS
GCCATGGCAAGCGAAAGAAA+AGG 0.604827 3:-38626848 MsaT012993.1:CDS
AGATTCTGGTAGAAGCGCAA+TGG 0.611720 3:-38628514 MsaT012993.1:CDS
AGCACTAAAGCTTCAAATGG+AGG 0.632582 3:-38627430 MsaT012993.1:CDS
TCGTCAAAGACTAAAGGACC+TGG 0.651924 3:+38626910 None:intergenic
GCAATGGATCCTGGTAGTGG+AGG 0.656586 3:-38628498 MsaT012993.1:CDS
GGGGAAAACACTCACGATCG+GGG 0.661843 3:+38628373 None:intergenic
CTTGTACACCCATAACTCTG+AGG 0.715783 3:+38628149 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 38626819 38628607 38626819 ID=MsaG012993
Chr3 mRNA 38626819 38628607 38626819 ID=MsaT012993.1;Parent=MsaG012993
Chr3 exon 38626819 38626941 38626819 ID=MsaT012993.1.exon5;Parent=MsaT012993.1
Chr3 CDS 38626819 38626941 38626819 ID=cds.MsaT012993.1;Parent=MsaT012993.1
Chr3 exon 38627401 38627470 38627401 ID=MsaT012993.1.exon4;Parent=MsaT012993.1
Chr3 CDS 38627401 38627470 38627401 ID=cds.MsaT012993.1;Parent=MsaT012993.1
Chr3 exon 38627773 38627805 38627773 ID=MsaT012993.1.exon3;Parent=MsaT012993.1
Chr3 CDS 38627773 38627805 38627773 ID=cds.MsaT012993.1;Parent=MsaT012993.1
Chr3 exon 38628109 38628190 38628109 ID=MsaT012993.1.exon2;Parent=MsaT012993.1
Chr3 CDS 38628109 38628190 38628109 ID=cds.MsaT012993.1;Parent=MsaT012993.1
Chr3 exon 38628388 38628607 38628388 ID=MsaT012993.1.exon1;Parent=MsaT012993.1
Chr3 CDS 38628388 38628607 38628388 ID=cds.MsaT012993.1;Parent=MsaT012993.1
Gene Sequence

>MsaT012993.1

ATGTATCATCCAACGAATGTTGCTGATTCGAGTTTAGTTGGAAGCAATCCATTAGTTAATGGTCAACATATAGATTCTGGTAGAAGCGCAATGGATCCTGGTAGTGGAGGAAACAGTCTTGCCAACAACTCTATTCTCAACTCAAAGCAACTCTTGCGTTGGACACATGAGCTACATGAACGCTTTGTTGATGCTGTGGCTCAACTTGGTGGCCCCGATCGTGCCACACCCAAAGGTGTCCTCAGAGTTATGGGTGTACAAGGCTTAACCATTTACCATGTTAAGAGCCATTTGCAGGTAATAATGGATATGCTTTCCATTCTAGATGGTTCATCGGGGATGCAGATTACTGAAGCACTAAAGCTTCAAATGGAGGTACAGAAGCGACTACATGAACAATTGGAGGATAGTGAACCAGGTCCTTTAGTCTTTGACGACCAACCATCATCACCAAGATCACCACTCTCAACAAAAAAGCCATGGCAAGCGAAAGAAAAGGATTTGTATGTAATGATGCCCTACTCCTAG

Protein sequence

>MsaT012993.1

MYHPTNVADSSLVGSNPLVNGQHIDSGRSAMDPGSGGNSLANNSILNSKQLLRWTHELHERFVDAVAQLGGPDRATPKGVLRVMGVQGLTIYHVKSHLQVIMDMLSILDGSSGMQITEALKLQMEVQKRLHEQLEDSEPGPLVFDDQPSSPRSPLSTKKPWQAKEKDLYVMMPYS*