AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013019


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013019 MtrunA17_Chr5g0404321 75.309 243 25 3 1 210 1 241 1.10e-121 351
MsaG013019 MtrunA17_Chr8g0373001 56.846 241 71 3 1 210 1 239 1.37e-90 271
MsaG013019 MtrunA17_Chr8g0373361 48.523 237 90 5 1 210 11 242 2.77e-70 219
MsaG013019 MtrunA17_Chr1g0155301 45.594 261 89 6 1 210 1 259 4.67e-67 211
MsaG013019 MtrunA17_Chr2g0278511 44.980 249 92 9 1 210 1 243 7.03e-57 186
MsaG013019 MtrunA17_Chr2g0286441 46.948 213 103 3 4 210 17 225 1.01e-56 184
MsaG013019 MtrunA17_Chr1g0201891 43.496 246 97 7 1 210 1 240 6.72e-56 183
MsaG013019 MtrunA17_Chr1g0156151 46.729 214 79 6 1 181 1 212 4.11e-54 172
MsaG013019 MtrunA17_Chr2g0315641 41.667 216 110 4 4 205 10 223 3.81e-50 166
MsaG013019 MtrunA17_Chr8g0373331 41.494 241 66 6 1 210 1 197 1.54e-47 159
MsaG013019 MtrunA17_Chr1g0196071 45.989 187 92 3 25 205 47 230 4.60e-47 159
MsaG013019 MtrunA17_Chr2g0315651 40.097 207 111 3 5 204 14 214 7.64e-45 152
MsaG013019 MtrunA17_Chr3g0091401 69.792 96 23 1 65 160 9 98 2.20e-36 123
MsaG013019 MtrunA17_Chr8g0373341 41.553 219 53 7 1 170 1 193 1.67e-34 125
MsaG013019 MtrunA17_Chr8g0373351 37.079 178 45 8 25 198 16 130 7.86e-20 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013019 AT2G18280.2 50.847 236 85 4 1 210 1 231 5.86e-74 229
MsaG013019 AT2G18280.1 50.847 236 85 4 1 210 1 231 5.86e-74 229
MsaG013019 AT1G76900.2 43.478 253 94 7 1 210 1 247 2.90e-55 182
MsaG013019 AT1G76900.1 43.478 253 94 7 1 210 1 247 2.90e-55 182
MsaG013019 AT1G76900.3 43.478 253 94 7 1 210 1 247 2.90e-55 182
MsaG013019 AT1G25280.1 44.444 252 95 6 1 210 1 249 1.48e-54 180
MsaG013019 AT1G47270.3 49.180 183 76 4 25 200 41 213 6.88e-54 177
MsaG013019 AT1G47270.1 49.180 183 76 4 25 200 66 238 1.20e-53 177
MsaG013019 AT1G47270.2 49.180 183 76 4 25 200 65 237 1.22e-53 177
MsaG013019 AT1G53320.1 46.114 193 96 2 24 210 40 230 1.37e-52 173
MsaG013019 AT2G47900.3 47.423 194 92 3 25 210 49 240 3.43e-51 171
MsaG013019 AT2G47900.2 47.668 193 92 3 25 210 49 239 5.47e-51 170
MsaG013019 AT2G47900.1 47.668 193 92 3 25 210 49 239 5.47e-51 170
MsaG013019 AT1G43640.1 43.005 193 98 5 27 210 54 243 2.35e-48 164
MsaG013019 AT3G06380.1 41.892 222 111 6 1 210 1 216 1.76e-46 157
MsaG013019 AT5G18680.1 46.073 191 93 3 27 210 38 225 4.59e-44 151
MsaG013019 AT1G43640.2 43.333 180 90 5 40 210 1 177 1.30e-43 150
MsaG013019 AT5G18680.2 46.154 130 67 1 81 210 7 133 1.25e-26 103
MsaG013019 AT3G06380.2 46.032 126 62 3 85 210 5 124 2.74e-26 102
MsaG013019 AT1G61940.2 44.048 84 41 1 34 111 4 87 1.47e-19 84.3
MsaG013019 AT1G25280.3 50.704 71 34 1 141 210 1 71 5.46e-12 63.9
MsaG013019 AT1G25280.2 50.704 71 34 1 141 210 1 71 5.46e-12 63.9
MsaG013019 AT1G61940.1 46.552 58 25 1 34 85 4 61 6.98e-11 60.5

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTCAGGTCTAACATTT+TGG 0.098131 3:-39273915 MsaT013019.1:CDS
CTCCTACTGGACCGGCTATT+TGG 0.181607 3:+39273833 None:intergenic
TCTGCGTATCTATTGTATTA+TGG 0.232029 3:-39274070 MsaT013019.1:CDS
CCAGTAGGAGATTTCTTTCT+AGG 0.241845 3:-39273820 MsaT013019.1:CDS
TCCTATTTCGTTAAATCAGC+TGG 0.277516 3:-39274143 MsaT013019.1:CDS
CGTCAACCTTCCACACTCTT+CGG 0.358084 3:+39274168 None:intergenic
CACAGACTTAATCATATCTA+TGG 0.360821 3:-39273978 MsaT013019.1:CDS
GTCAACCTTCCACACTCTTC+GGG 0.361120 3:+39274169 None:intergenic
CCCAGCTGATTTAACGAAAT+AGG 0.367273 3:+39274142 None:intergenic
TAGAAGAGACTGAGACTTCT+TGG 0.372030 3:-39274252 MsaT013019.1:CDS
GTGCTCCTGTGTGTGAATCT+TGG 0.372852 3:-39274222 MsaT013019.1:CDS
TAGTTATATGCTTGAACTCT+TGG 0.377661 3:+39273788 None:intergenic
AGGCAACCTCCACACGGATC+TGG 0.416160 3:-39273863 MsaT013019.1:CDS
CTCCGATACAATGCTTCATC+AGG 0.436765 3:-39274108 MsaT013019.1:CDS
ATATGAGCAATTCAGCTCAT+AGG 0.461752 3:+39273749 None:intergenic
ACCAATTTAGCAGGGACAAT+GGG 0.465957 3:-39274317 MsaT013019.1:intron
TAGGAGATTTCTTTCTAGGC+AGG 0.469861 3:-39273816 MsaT013019.1:CDS
CTCCTGTGTGTGAATCTTGG+AGG 0.478005 3:-39274219 MsaT013019.1:CDS
AACTCAAGGAGATGAGGGAT+GGG 0.484739 3:-39274435 MsaT013019.1:CDS
CGTTCCAGCAATGCGTATGT+TGG 0.488866 3:-39273938 MsaT013019.1:CDS
CCTATTTCGTTAAATCAGCT+GGG 0.495267 3:-39274142 MsaT013019.1:CDS
GAACTCAAGGAGATGAGGGA+TGG 0.505860 3:-39274436 MsaT013019.1:CDS
TGTTGTTGAGAAGCATAGTA+CGG 0.514474 3:-39274459 MsaT013019.1:CDS
CCTAGAAAGAAATCTCCTAC+TGG 0.516235 3:+39273820 None:intergenic
TCACTATATACGACATTGAC+AGG 0.521151 3:-39273883 MsaT013019.1:CDS
GTATTATGGACTGGTGCCAT+TGG 0.546205 3:-39274056 MsaT013019.1:CDS
TCCTGTGTGTGAATCTTGGA+GGG 0.550726 3:-39274218 MsaT013019.1:CDS
AAAGAAATCTCCTACTGGAC+CGG 0.552476 3:+39273825 None:intergenic
AAGCATAGTACGGGAACTCA+AGG 0.555180 3:-39274449 MsaT013019.1:CDS
AAGGAGATGAGGGATGGGAT+TGG 0.557067 3:-39274430 MsaT013019.1:CDS
TGCTACTTGATATAATTCGA+AGG 0.558581 3:-39274276 MsaT013019.1:CDS
AACCAATTTAGCAGGGACAA+TGG 0.568016 3:-39274318 MsaT013019.1:intron
AGATATGATTAAGTCTGTGC+CGG 0.568041 3:+39273982 None:intergenic
CGATACAATGCTTCATCAGG+AGG 0.568935 3:-39274105 MsaT013019.1:CDS
GTATCTATTGTATTATGGAC+TGG 0.570769 3:-39274065 MsaT013019.1:CDS
AATGTCGTATATAGTGAACT+TGG 0.574240 3:+39273889 None:intergenic
GCTACTTGATATAATTCGAA+GGG 0.575810 3:-39274275 MsaT013019.1:CDS
GTTGTTGAGAAGCATAGTAC+GGG 0.578798 3:-39274458 MsaT013019.1:CDS
CTGGAATTCAACCAAATAGC+CGG 0.579251 3:-39273844 MsaT013019.1:CDS
TACGGGAACTCAAGGAGATG+AGG 0.591073 3:-39274441 MsaT013019.1:CDS
CTCCTGATGAAGCATTGTAT+CGG 0.592254 3:+39274106 None:intergenic
CTGAACAATCTCTTGTGTAA+CGG 0.594307 3:+39274195 None:intergenic
ACGGGAACTCAAGGAGATGA+GGG 0.594362 3:-39274440 MsaT013019.1:CDS
ATGCGTATGTTGGAAAACTC+AGG 0.599772 3:-39273928 MsaT013019.1:CDS
AGCAATTCAGCTCATAGGAA+TGG 0.600877 3:+39273754 None:intergenic
AGACTCCCGAAGAGTGTGGA+AGG 0.609764 3:-39274174 MsaT013019.1:CDS
TCACACACAGGAGCACAAGA+AGG 0.618611 3:+39274229 None:intergenic
CATTGTATCGGAGAATCACA+AGG 0.642254 3:+39274118 None:intergenic
GTTCAGACTCCCGAAGAGTG+TGG 0.642949 3:-39274178 MsaT013019.1:CDS
ATTGGTAATACGTCGAGGAG+AGG 0.649495 3:-39274412 MsaT013019.1:CDS
AACCAAATAGCCGGTCCAGT+AGG 0.657951 3:-39273835 MsaT013019.1:CDS
GCCCTCCAAGATTCACACAC+AGG 0.660123 3:+39274217 None:intergenic
TGAATTCCAGATCCGTGTGG+AGG 0.662267 3:+39273857 None:intergenic
GATACAATGCTTCATCAGGA+GGG 0.667883 3:-39274104 MsaT013019.1:CDS
ATTGACAGGCAACCTCCACA+CGG 0.696401 3:-39273869 MsaT013019.1:CDS
ATGGGATTGGTAATACGTCG+AGG 0.700501 3:-39274417 MsaT013019.1:CDS
GGTTGAATTCCAGATCCGTG+TGG 0.703459 3:+39273854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 39273760 39274482 39273760 ID=MsaG013019
Chr3 mRNA 39273760 39274482 39273760 ID=MsaT013019.1;Parent=MsaG013019
Chr3 exon 39273760 39274329 39273760 ID=MsaT013019.1.exon2;Parent=MsaT013019.1
Chr3 CDS 39273760 39274329 39273760 ID=cds.MsaT013019.1;Parent=MsaT013019.1
Chr3 exon 39274411 39274482 39274411 ID=MsaT013019.1.exon1;Parent=MsaT013019.1
Chr3 CDS 39274411 39274482 39274411 ID=cds.MsaT013019.1;Parent=MsaT013019.1
Gene Sequence

>MsaT013019.1

ATGTTGTTGAGAAGCATAGTACGGGAACTCAAGGAGATGAGGGATGGGATTGGTAATACGTCGAGGAGAGGTCAGGGACAATGGGTGAATATACAATCTGAATTGCTACTTGATATAATTCGAAGGGTAGAAGAGACTGAGACTTCTTGGCCTTCTTGTGCTCCTGTGTGTGAATCTTGGAGGGCCGTTACACAAGAGATTGTTCAGACTCCCGAAGAGTGTGGAAGGTTGACGTTTCCTATTTCGTTAAATCAGCTGGGTCCTTGTGATTCTCCGATACAATGCTTCATCAGGAGGGATAGAGAAACTTCTGCGTATCTATTGTATTATGGACTGGTGCCATTGGAAAATGAGACTGATAAGATGTTATTAGCTGCCAAAAAGATAAGGAAGACAGCCGGCACAGACTTAATCATATCTATGGTTGCAGTCGATTTTTCTCGTTCCAGCAATGCGTATGTTGGAAAACTCAGGTCTAACATTTTGGGAACCAAGTTCACTATATACGACATTGACAGGCAACCTCCACACGGATCTGGAATTCAACCAAATAGCCGGTCCAGTAGGAGATTTCTTTCTAGGCAGGTGTCTCCAAGAGTTCAAGCATATAACTATATTGTTAGCACCATTCCTATGAGCTGA

Protein sequence

>MsaT013019.1

MLLRSIVRELKEMRDGIGNTSRRGQGQWVNIQSELLLDIIRRVEETETSWPSCAPVCESWRAVTQEIVQTPEECGRLTFPISLNQLGPCDSPIQCFIRRDRETSAYLLYYGLVPLENETDKMLLAAKKIRKTAGTDLIISMVAVDFSRSSNAYVGKLRSNILGTKFTIYDIDRQPPHGSGIQPNSRSSRRFLSRQVSPRVQAYNYIVSTIPMS*