AlfalfaGEDB | MsaG013064

M. sativa ssp. caerulea / MsaG013064

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG013064	XP_039685680.1	86.923	650	58	2	290	912	79	728	0.0	1172

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG013064	sp\|Q9SZL8\|FRS5_ARATH	26.244	583	339	16	311	865	118	637	1.14e-53	204

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG013064	tr\|D3YBB9\|D3YBB9_TRIRP	76.947	655	111	3	290	912	111	757	0.0	1038

TFs/TRs:

Gene ID	Type	Classification
MsaG013064	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000519	MsaG013064	0.813433	4.422827e-51	8.219819e-49
MsaG001179	MsaG013064	0.809177	3.672144e-50	6.147558e-48
MsaG001413	MsaG013064	0.832503	1.647547e-55	5.126019e-53
MsaG001414	MsaG013064	0.832954	1.275212e-55	4.020007e-53
MsaG001415	MsaG013064	0.836350	1.801199e-56	6.283098e-54
MsaG002016	MsaG013064	0.807457	8.510433e-50	1.367011e-47
MsaG003288	MsaG013064	0.818875	2.724139e-52	5.816435e-50
MsaG005205	MsaG013064	0.802200	1.054552e-48	1.498610e-46
MsaG006533	MsaG013064	0.829837	7.395043e-55	2.130243e-52
MsaG006601	MsaG013064	0.811211	1.344408e-50	2.364750e-48
MsaG007067	MsaG013064	0.802351	9.820022e-49	1.400420e-46
MsaG008103	MsaG013064	0.805658	2.031445e-49	3.127255e-47
MsaG008274	MsaG013064	0.802894	7.594179e-49	1.096527e-46
MsaG010299	MsaG013064	0.801045	1.814143e-48	2.511359e-46
MsaG011505	MsaG013064	0.813262	4.818693e-51	8.917120e-49
MsaG012273	MsaG013064	0.834979	3.991314e-56	1.336083e-53
MsaG012326	MsaG013064	0.801556	1.427882e-48	1.999633e-46
MsaG012341	MsaG013064	0.807497	8.344898e-50	1.341722e-47
MsaG012581	MsaG013064	0.823087	2.952662e-53	7.051049e-51
MsaG044380	MsaG013064	0.812340	7.653583e-51	1.384156e-48
MsaG045890	MsaG013064	0.820333	1.270893e-52	2.820019e-50
MsaG046307	MsaG013064	0.824873	1.130508e-53	2.833906e-51
MsaG010012	MsaG013064	0.815794	1.334786e-51	2.632648e-49
MsaG013064	MsaG019037	0.803455	5.820524e-49	8.513802e-47
MsaG013064	MsaG019920	0.803962	4.574318e-49	6.770083e-47
MsaG013064	MsaG031903	0.800291	2.580656e-48	3.512151e-46
MsaG013064	MsaG030523	0.822000	5.269068e-53	1.221922e-50
MsaG013064	MsaG038716	0.814248	2.930111e-51	5.558026e-49
MsaG013064	MsaG037163	0.802118	1.095756e-48	1.554264e-46
MsaG012933	MsaG013064	0.812524	6.980781e-51	1.268275e-48
MsaG018183	MsaG013064	0.828514	1.542637e-54	4.280001e-52
MsaG018343	MsaG013064	0.811307	1.281433e-50	2.259371e-48
MsaG018452	MsaG013064	0.830625	4.757217e-55	1.401543e-52
MsaG022058	MsaG013064	0.819395	2.077627e-52	4.497111e-50
MsaG022059	MsaG013064	0.802620	8.646209e-49	1.240642e-46
MsaG022438	MsaG013064	0.808334	5.548564e-50	9.102267e-48
MsaG022441	MsaG013064	0.825906	6.455064e-54	1.665039e-51
MsaG023619	MsaG013064	0.804944	2.861568e-49	4.332666e-47
MsaG023914	MsaG013064	0.813857	3.570628e-51	6.707034e-49
MsaG023837	MsaG013064	0.806369	1.441992e-49	2.257453e-47
MsaG024026	MsaG013064	0.811514	1.156416e-50	2.049212e-48
MsaG029388	MsaG013064	0.847951	1.579869e-59	7.987973e-57
MsaG029862	MsaG013064	0.811239	1.325950e-50	2.333902e-48
MsaG030840	MsaG013064	0.816023	1.187489e-51	2.355790e-49
MsaG031436	MsaG013064	0.810718	1.717314e-50	2.984424e-48
MsaG032998	MsaG013064	0.815372	1.655242e-51	3.230073e-49
MsaG040775	MsaG013064	0.819075	2.454879e-52	5.269242e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG013064	MtrunA17_Chr3g0082121	28.992	645	358	15	293	909	70	642	4.90e-81	276
MsaG013064	MtrunA17_Chr2g0324011	29.542	589	331	12	301	865	43	571	1.43e-79	270
MsaG013064	MtrunA17_Chr1g0202341	27.951	576	325	15	313	862	98	609	1.32e-64	230
MsaG013064	MtrunA17_Chr1g0203721	25.201	623	375	14	311	905	108	667	7.58e-60	220
MsaG013064	MtrunA17_Chr5g0448761	26.644	593	350	17	311	876	105	639	3.55e-57	211
MsaG013064	MtrunA17_Chr8g0363841	26.667	585	330	15	312	865	64	580	6.40e-57	208
MsaG013064	MtrunA17_Chr2g0296781	26.701	588	358	15	348	908	61	602	7.47e-57	209
MsaG013064	MtrunA17_Chr6g0477261	27.872	531	308	15	407	908	7	491	8.43e-57	207
MsaG013064	MtrunA17_Chr1g0164591	32.911	316	180	5	358	650	1	307	9.19e-55	193
MsaG013064	MtrunA17_Chr6g0458391	25.557	583	316	14	311	862	270	765	7.65e-52	195
MsaG013064	MtrunA17_Chr3g0088371	85.263	95	14	0	818	912	1	95	3.34e-51	178
MsaG013064	MtrunA17_Chr5g0441581	25.880	568	348	13	344	889	93	609	4.66e-51	191
MsaG013064	MtrunA17_Chr2g0329031	26.047	549	306	18	344	858	75	557	2.21e-47	181
MsaG013064	MtrunA17_Chr3g0118611	27.021	470	276	11	448	896	111	534	3.60e-47	181
MsaG013064	MtrunA17_Chr2g0287651	24.216	574	351	11	312	862	120	632	7.47e-47	181
MsaG013064	MtrunA17_Chr7g0258861	35.714	266	154	5	301	563	43	294	3.25e-45	165
MsaG013064	MtrunA17_Chr1g0198761	27.808	543	298	17	354	860	126	610	4.05e-45	174
MsaG013064	MtrunA17_Chr1g0207551	26.596	470	262	11	454	895	204	618	5.14e-45	174
MsaG013064	MtrunA17_Chr3g0080351	26.157	497	292	11	440	912	224	669	4.34e-43	169
MsaG013064	MtrunA17_Chr5g0440451	26.077	441	254	10	448	864	214	606	3.38e-42	167
MsaG013064	MtrunA17_Chr1g0196541	24.059	478	302	8	407	862	118	556	3.95e-42	165
MsaG013064	MtrunA17_Chr6g0475591	88.312	77	9	0	415	491	1	77	8.33e-40	141
MsaG013064	MtrunA17_Chr2g0287061	24.525	579	311	16	311	865	228	704	1.35e-39	158
MsaG013064	MtrunA17_Chr1g0203051	22.121	547	351	7	312	837	124	616	6.31e-39	154
MsaG013064	MtrunA17_Chr2g0276841	26.014	592	332	21	312	869	117	636	6.18e-38	152
MsaG013064	MtrunA17_Chr2g0290391	25.000	556	317	15	345	860	116	611	1.09e-36	148
MsaG013064	MtrunA17_Chr5g0448511	24.888	446	266	10	443	865	1	400	1.58e-35	142
MsaG013064	MtrunA17_Chr2g0291591	25.758	396	248	9	341	701	117	501	2.08e-35	142
MsaG013064	MtrunA17_Chr5g0431001	26.139	417	254	12	292	675	93	488	2.09e-32	132
MsaG013064	MtrunA17_Chr2g0291581	23.869	641	375	17	253	862	63	621	5.28e-32	133
MsaG013064	MtrunA17_Chr1g0178241	70.513	78	23	0	4	81	28	105	1.64e-27	119
MsaG013064	MtrunA17_Chr2g0294841	26.420	405	249	11	312	684	92	479	1.96e-26	116
MsaG013064	MtrunA17_Chr7g0229821	56.790	81	33	1	1	81	15	93	4.99e-22	93.6
MsaG013064	MtrunA17_Chr1g0172971	85.185	54	8	0	290	343	64	117	4.64e-21	98.2
MsaG013064	MtrunA17_Chr7g0243381	38.095	168	86	4	154	318	111	263	2.21e-20	92.8
MsaG013064	MtrunA17_Chr7g0229881	55.556	81	34	1	1	81	15	93	1.66e-19	91.7
MsaG013064	MtrunA17_Chr7g0271301	45.946	74	38	1	6	79	19	90	2.75e-11	65.9
MsaG013064	MtrunA17_Chr1g0170041	41.584	101	46	3	226	318	13	108	8.94e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG013064	AT4G38180.1	26.244	583	339	16	311	865	118	637	1.16e-54	204
MsaG013064	AT4G15090.1	26.819	481	277	9	454	910	224	653	1.19e-47	183
MsaG013064	AT4G15090.5	26.819	481	277	9	454	910	224	653	1.19e-47	183
MsaG013064	AT4G15090.3	26.819	481	277	9	454	910	224	653	1.19e-47	183
MsaG013064	AT4G15090.2	26.819	481	277	9	454	910	224	653	1.19e-47	183
MsaG013064	AT4G15090.4	26.819	481	277	9	454	910	224	653	1.19e-47	183
MsaG013064	AT2G27110.3	25.151	497	298	12	438	910	34	480	1.07e-42	167
MsaG013064	AT2G27110.2	25.553	497	296	13	438	910	179	625	1.23e-42	168
MsaG013064	AT2G27110.1	25.553	497	296	13	438	910	179	625	1.23e-42	168
MsaG013064	AT3G22170.2	24.896	482	282	11	453	908	232	659	3.37e-40	160
MsaG013064	AT3G22170.1	24.896	482	282	11	453	908	232	659	3.37e-40	160
MsaG013064	AT1G76320.1	25.885	537	316	14	351	862	65	544	3.89e-40	159
MsaG013064	AT1G76320.4	25.885	537	316	14	351	862	65	544	3.89e-40	159
MsaG013064	AT1G76320.2	25.885	537	316	14	351	862	65	544	4.27e-40	159
MsaG013064	AT1G76320.3	25.885	537	316	14	351	862	65	544	6.21e-40	158
MsaG013064	AT3G06250.2	25.056	443	264	10	439	861	309	703	2.75e-38	154
MsaG013064	AT3G06250.1	25.056	443	264	10	439	861	309	703	2.75e-38	154
MsaG013064	AT3G06250.3	25.056	443	264	10	439	861	309	703	2.75e-38	154
MsaG013064	AT4G38170.1	24.235	425	249	11	462	862	24	399	3.75e-36	145
MsaG013064	AT1G10240.2	25.000	552	315	19	345	859	115	604	4.12e-34	140
MsaG013064	AT1G10240.1	25.000	552	315	19	345	859	115	604	4.12e-34	140
MsaG013064	AT2G32250.2	23.125	640	357	17	296	910	68	597	9.57e-34	140
MsaG013064	AT2G32250.4	23.125	640	357	17	296	910	68	597	9.57e-34	140
MsaG013064	AT2G32250.5	23.125	640	357	17	296	910	68	597	1.01e-33	139
MsaG013064	AT2G32250.6	23.125	640	357	17	296	910	68	597	1.24e-33	139
MsaG013064	AT2G32250.1	23.125	640	357	17	296	910	68	597	1.24e-33	139
MsaG013064	AT2G32250.3	23.125	640	357	17	296	910	68	597	1.24e-33	139
MsaG013064	AT2G32250.7	23.125	640	357	17	296	910	27	556	1.39e-33	139
MsaG013064	AT2G32250.8	23.125	640	357	17	296	910	27	556	1.62e-33	139
MsaG013064	AT5G18960.2	24.944	445	259	13	441	861	334	727	3.06e-33	138
MsaG013064	AT5G18960.1	24.944	445	259	13	441	861	334	727	3.06e-33	138
MsaG013064	AT5G28530.1	23.794	622	357	18	296	878	84	627	4.09e-32	134
MsaG013064	AT1G80010.1	24.424	434	261	11	454	862	270	661	2.04e-31	132
MsaG013064	AT5G28530.3	23.633	622	354	19	296	878	84	623	4.57e-31	130
MsaG013064	AT5G28530.2	23.633	622	354	19	296	878	84	623	4.57e-31	130
MsaG013064	AT1G52520.1	22.757	602	358	19	293	862	112	638	5.72e-28	121
MsaG013064	AT4G19990.1	27.652	264	167	4	446	685	106	369	5.96e-25	111
MsaG013064	AT4G19990.5	27.652	264	167	4	446	685	163	426	6.56e-25	112
MsaG013064	AT4G19990.3	27.652	264	167	4	446	685	163	426	6.75e-25	111
MsaG013064	AT4G19990.4	27.652	264	167	4	446	685	163	426	6.75e-25	111
MsaG013064	AT4G19990.6	27.652	264	167	4	446	685	163	426	6.84e-25	112
MsaG013064	AT4G19990.2	27.652	264	167	4	446	685	163	426	6.84e-25	112
MsaG013064	AT4G19990.7	27.652	264	167	4	446	685	177	440	7.86e-25	111
MsaG013064	AT5G28530.4	24.074	378	195	13	528	878	252	564	1.21e-14	78.6

Find 154 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGAACTTTCCTCTTATC+TGG	0.128821	3:-40179851	None:intergenic
GCCCTCTCTGGCTCGGTTTC+AGG	0.134191	3:+40178580	MsaT013064.1:CDS
TAAATTTCAGCTGCCTTTAT+TGG	0.156227	3:+40182222	MsaT013064.1:intron
TATCGTCTGTGGTTTCTTAT+TGG	0.203510	3:-40182349	None:intergenic
CTTTAGTCATCTCAACATTA+TGG	0.239089	3:+40182770	MsaT013064.1:CDS
CACTACTACAGATGAAATTC+TGG	0.252486	3:+40179826	MsaT013064.1:CDS
CTTGCTTTGCATTTATTCAT+AGG	0.265760	3:+40182910	MsaT013064.1:CDS
GTCTCCTCCTCCATTCTTAG+AGG	0.265827	3:+40179745	MsaT013064.1:CDS
ACTAAGTACTCATCAAAATT+AGG	0.286638	3:-40179779	None:intergenic
TCTGATGTTGTCCTGTTGTT+AGG	0.288908	3:-40181015	None:intergenic
GCCTTCCACTGTTGATGTTT+TGG	0.290838	3:+40183061	MsaT013064.1:CDS
GCGGTGAACCCACCGGCATC+TGG	0.304530	3:+40178541	MsaT013064.1:CDS
TTACTTCTCGAAATGATCTA+AGG	0.311482	3:-40181206	None:intergenic
TCAAGCTCAATCTAACAATA+TGG	0.313798	3:+40179430	MsaT013064.1:CDS
TTGTTGGATTCCCAGCCTTC+TGG	0.314278	3:-40179562	None:intergenic
CAACTAGATTATTCTCTATT+CGG	0.323831	3:+40181522	MsaT013064.1:CDS
CTAAGGTAACACGAATGATT+TGG	0.324322	3:-40181189	None:intergenic
TTTAAGCCTGAAAACCAAAA+AGG	0.346249	3:-40181333	None:intergenic
ATGATTGTCAAAACCAATAA+AGG	0.364908	3:-40182235	None:intergenic
GTTCACAACATTGCTCAATC+TGG	0.380274	3:+40179374	MsaT013064.1:CDS
CATGTCATATGTTTGAAAAC+AGG	0.389937	3:+40183186	MsaT013064.1:CDS
TCATGCTGAGTTGGACTTCA+AGG	0.393220	3:+40182812	MsaT013064.1:CDS
TTCCTTCAAATACTATTATG+AGG	0.399583	3:+40182944	MsaT013064.1:CDS
GAGAACGTCTAATGAAGTTA+TGG	0.402471	3:+40181281	MsaT013064.1:CDS
CTTCAGTGTTTGAGTTGCTA+AGG	0.403463	3:-40178749	None:intergenic
CTCAAACAACTCAAAAGAAA+TGG	0.406124	3:-40179597	None:intergenic
CTCCATTCTTAGAGGTTCCT+AGG	0.410638	3:+40179753	MsaT013064.1:CDS
TGCACAAGCCCTCTCTGGCT+CGG	0.418976	3:+40178573	MsaT013064.1:CDS
GACCGCATCAGCTGCCTGTT+GGG	0.418984	3:-40182864	None:intergenic
TCCTTTGCACAAGCCCTCTC+TGG	0.420227	3:+40178568	MsaT013064.1:CDS
ATCGAACAAACAATATATAT+CGG	0.421135	3:+40181571	MsaT013064.1:CDS
AAAACACCTGGAAAACTATT+CGG	0.428349	3:-40182408	None:intergenic
CGAGAAGTAAATGAAGTTCA+AGG	0.430014	3:+40181219	MsaT013064.1:CDS
AGCTGGAATATCTTTGAAGT+TGG	0.433004	3:+40181101	MsaT013064.1:CDS
TACTCACATTACAGTTGATA+AGG	0.434062	3:+40179670	MsaT013064.1:CDS
AACAATATATATCGGCCATT+AGG	0.437083	3:+40181579	MsaT013064.1:CDS
GATCTTCAAAACTTGAGAAA+AGG	0.437597	3:-40183103	None:intergenic
TCTCAAGCAGCATATGAATC+TGG	0.439032	3:+40178667	MsaT013064.1:CDS
GAAAGGGTTGGTTGTAAAGC+TGG	0.447509	3:+40181084	MsaT013064.1:CDS
TTGCTTTGCATTTATTCATA+GGG	0.448231	3:+40182911	MsaT013064.1:CDS
ATTTCATCTGTAGTAGTGAT+AGG	0.450643	3:-40179821	None:intergenic
ATGTGTATAAGATATATAGA+TGG	0.452819	3:+40181136	MsaT013064.1:CDS
GAATGATTTGGTTTATGTAA+TGG	0.457469	3:-40181177	None:intergenic
GATTGTGGGTGATTCAGTTC+GGG	0.459018	3:+40178636	MsaT013064.1:CDS
GAAGTTATGGGTCAAGGAGT+TGG	0.459248	3:+40181294	MsaT013064.1:CDS
CTTCAAAACATGTCGACATA+AGG	0.462702	3:-40183213	None:intergenic
GCTCTTATAAATTCTAAAGC+TGG	0.462891	3:+40181249	MsaT013064.1:CDS
AGAACGTCTAATGAAGTTAT+GGG	0.466325	3:+40181282	MsaT013064.1:CDS
TCCTCCACATTTAGACTGAT+AGG	0.466714	3:-40179698	None:intergenic
GTTTGCTTAATCCTAACAAC+AGG	0.467124	3:+40181004	MsaT013064.1:CDS
GCTTCCTCCGAAGGTGATTG+TGG	0.469693	3:+40178621	MsaT013064.1:CDS
GAGACAAGTGTTCCCTCTCT+AGG	0.472534	3:-40179727	None:intergenic
TTGAAGATCTTGGAGTTCCT+AGG	0.473596	3:+40183230	MsaT013064.1:CDS
ACAAAATAAGAAATCTGATA+AGG	0.474392	3:+40180933	MsaT013064.1:CDS
AGGAAAGTTCAATTCATTGA+AGG	0.474885	3:+40179860	MsaT013064.1:CDS
TGATTGTGGGTGATTCAGTT+CGG	0.475999	3:+40178635	MsaT013064.1:CDS
TATGAGGAGGACATAACAAA+AGG	0.476915	3:+40182960	MsaT013064.1:CDS
AATCACGTCGCAATCACGAC+TGG	0.480850	3:-40180246	None:intergenic
CTCCTCATAATAGTATTTGA+AGG	0.485089	3:-40182946	None:intergenic
AAATTCTGGGCCCAGATAAG+AGG	0.486330	3:+40179840	MsaT013064.1:CDS
ATTGAACTTTCCTCTTATCT+GGG	0.492798	3:-40179850	None:intergenic
CGTTATGTTTGTAACAAACA+AGG	0.494754	3:+40180967	MsaT013064.1:CDS
TTCTCTTGGTTCTATGGCGG+CGG	0.495367	3:+40178522	None:intergenic
ATTGTGACAAACCAGAAGGC+TGG	0.500515	3:+40179551	MsaT013064.1:CDS
TGTCTTCTGCAGCATCAACT+TGG	0.502977	3:-40181467	None:intergenic
GGAGGCTAGGCCAGCTGACA+TGG	0.503073	3:+40179643	MsaT013064.1:CDS
TTACAACCAACCCTTTCCTC+AGG	0.505082	3:-40181078	None:intergenic
AACTCAAAAGAAATGGATAC+AGG	0.506566	3:-40179590	None:intergenic
GAGGGATAATCATGCTGAGT+TGG	0.508852	3:+40182803	MsaT013064.1:CDS
TTCAGTGTTTGAGTTGCTAA+GGG	0.509688	3:-40178748	None:intergenic
TAACAAACAAGGTTTCAAGA+AGG	0.511682	3:+40180978	MsaT013064.1:CDS
TCCATTCTTAGAGGTTCCTA+GGG	0.512997	3:+40179754	MsaT013064.1:CDS
TCCCTAGGAACCTCTAAGAA+TGG	0.514169	3:-40179755	None:intergenic
ACATTATGGTTCAGAGAATG+AGG	0.515275	3:+40182784	MsaT013064.1:CDS
TCTTACTCAGCTTCCTCCGA+AGG	0.518453	3:+40178612	MsaT013064.1:CDS
CGGTGAACCCACCGGCATCT+GGG	0.519134	3:+40178542	MsaT013064.1:CDS
TAAAGAATCGAACAAGTGAA+TGG	0.519618	3:-40182752	None:intergenic
TCATGATCGACTTGATGAAA+GGG	0.522852	3:+40183124	MsaT013064.1:CDS
CTTCCTCCGAAGGTGATTGT+GGG	0.525649	3:+40178622	MsaT013064.1:CDS
CATAATAGTATTTGAAGGAA+AGG	0.529686	3:-40182941	None:intergenic
TCATCAAAATTAGGATCCCT+AGG	0.530667	3:-40179770	None:intergenic
ATCATGATCGACTTGATGAA+AGG	0.531340	3:+40183123	MsaT013064.1:CDS
ACTACTACAGATGAAATTCT+GGG	0.531635	3:+40179827	MsaT013064.1:CDS
ATGTCATATGTTTGAAAACA+GGG	0.531727	3:+40183187	MsaT013064.1:CDS
TGGGCTTTGTTCATGGCACA+TGG	0.532868	3:+40182434	MsaT013064.1:CDS
GCAAAGGATTTCCCAGATGC+CGG	0.536904	3:-40178553	None:intergenic
CATAACAAAAGGGTCTGCCT+TGG	0.539378	3:+40182971	MsaT013064.1:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	3:-40182747	None:intergenic
ATGAGGAGGACATAACAAAA+GGG	0.542077	3:+40182961	MsaT013064.1:CDS
TGAGTAATTTCCATGTCAGC+TGG	0.546379	3:-40179653	None:intergenic
TGAATAAATGCAAAGCAAGC+TGG	0.546449	3:-40182906	None:intergenic
GAATCACCCACAATCACCTT+CGG	0.547161	3:-40178628	None:intergenic
AACACAATAACGCACTAGCC+TGG	0.552106	3:-40182380	None:intergenic
AACAAACAAGGTTTCAAGAA+GGG	0.554561	3:+40180979	MsaT013064.1:CDS
TTCACAAACTCAATAACCAA+TGG	0.554620	3:-40181042	None:intergenic
TTGTGACAAACCAGAAGGCT+GGG	0.556364	3:+40179552	MsaT013064.1:CDS
AGACAAGTGTTCCCTCTCTA+GGG	0.557209	3:-40179726	None:intergenic
CATTGCTCAATCTGGAAATG+AGG	0.558506	3:+40179382	MsaT013064.1:CDS
GACAATATGTTATAAAACGT+TGG	0.562457	3:+40183291	MsaT013064.1:CDS
AGGGTGAATCTCCTTTGTGA+AGG	0.565730	3:-40178729	None:intergenic
TAAGATATATAGATGGGATG+TGG	0.566406	3:+40181143	MsaT013064.1:CDS
GCCAGAGAGGGCTTGTGCAA+AGG	0.566856	3:-40178569	None:intergenic
GCGACGTGATTTCACCGTCT+AGG	0.570227	3:+40180258	MsaT013064.1:CDS
GGACCGCATCAGCTGCCTGT+TGG	0.570695	3:-40182865	None:intergenic
TCTAATGAAGTTATGGGTCA+AGG	0.571062	3:+40181288	MsaT013064.1:CDS
ACCTGAAACCGAGCCAGAGA+GGG	0.574647	3:-40178581	None:intergenic
GAATAACTCTGACACTGTTG+TGG	0.574750	3:+40179460	MsaT013064.1:CDS
GTCTTCAGTGCCAACATGGA+TGG	0.576478	3:-40183354	None:intergenic
GGGTGAATCTCCTTTGTGAA+GGG	0.577538	3:-40178728	None:intergenic
TCCAAAACATCAACAGTGGA+AGG	0.578008	3:-40183062	None:intergenic
GTTATGGGTCAAGGAGTTGG+AGG	0.578191	3:+40181297	MsaT013064.1:CDS
CAATCTGGAAATGAGGCAGT+TGG	0.579303	3:+40179389	MsaT013064.1:CDS
AAGGATTTCCCAGATGCCGG+TGG	0.582755	3:-40178550	None:intergenic
ATCCCAACAGGCAGCTGATG+CGG	0.586005	3:+40182862	MsaT013064.1:CDS
GCTTATCAGTGTAATCTACA+TGG	0.589625	3:+40178697	MsaT013064.1:CDS
CACTGTTGTGGAAGGTGGTG+AGG	0.592391	3:+40179472	MsaT013064.1:CDS
TATGATGATGCTGATAATGA+CGG	0.599988	3:+40183038	MsaT013064.1:CDS
TGTGTATAAGATATATAGAT+GGG	0.601002	3:+40181137	MsaT013064.1:CDS
TTGATATCGTTGTGCTTGAG+TGG	0.601464	3:-40183378	None:intergenic
TATGGCGGCGGTGAACCCAC+CGG	0.601591	3:+40178534	None:intergenic
GACAACATCAGACAGTCCAT+TGG	0.604607	3:+40181026	MsaT013064.1:CDS
CGGTGAGCAAACGTGAAGCA+AGG	0.605996	3:-40180208	None:intergenic
CTTCAAATACTATTATGAGG+AGG	0.607664	3:+40182947	MsaT013064.1:CDS
CTGAAATCGACCATCCATGT+TGG	0.607729	3:+40183344	MsaT013064.1:CDS
GGAACCTCTAAGAATGGAGG+AGG	0.609119	3:-40179749	None:intergenic
GCCTATCAGTCTAAATGTGG+AGG	0.610964	3:+40179697	MsaT013064.1:CDS
TGGAGGACACTTCCCTAGAG+AGG	0.611220	3:+40179714	MsaT013064.1:CDS
AACTCTGACACTGTTGTGGA+AGG	0.615703	3:+40179464	MsaT013064.1:CDS
ATGATGATGCTGATAATGAC+GGG	0.620534	3:+40183039	MsaT013064.1:CDS
TGCTATTGTGACAAACCAGA+AGG	0.621231	3:+40179547	MsaT013064.1:CDS
CTTAGCAACTCAAACACTGA+AGG	0.626856	3:+40178750	MsaT013064.1:CDS
AGGATTTCCCAGATGCCGGT+GGG	0.628718	3:-40178549	None:intergenic
GATGCCTATCAGTCTAAATG+TGG	0.629501	3:+40179694	MsaT013064.1:CDS
TGAAATTCGTGCAACCTAGA+CGG	0.630711	3:-40180272	None:intergenic
TGTCATATGTTTGAAAACAG+GGG	0.632114	3:+40183188	MsaT013064.1:CDS
ACAGTGTCAGAGTTATTCAA+AGG	0.632899	3:-40179455	None:intergenic
TCTGACACTGTTGTGGAAGG+TGG	0.634726	3:+40179467	MsaT013064.1:CDS
GGAGGACACTTCCCTAGAGA+GGG	0.640474	3:+40179715	MsaT013064.1:CDS
CATTATGGTTCAGAGAATGA+GGG	0.643278	3:+40182785	MsaT013064.1:CDS
CTAGGAACCTCTAAGAATGG+AGG	0.644669	3:-40179752	None:intergenic
TCACCCACAATCACCTTCGG+AGG	0.648244	3:-40178625	None:intergenic
TGGTCTGTATATATCGTCTG+TGG	0.648431	3:-40182360	None:intergenic
TATATTGCACATGACCACCT+AGG	0.649929	3:-40183247	None:intergenic
TGGTGTCTTCAGTGCCAACA+TGG	0.650133	3:-40183358	None:intergenic
GATTTCAGCTTATCGACAGA+CGG	0.652942	3:-40183329	None:intergenic
TGCATCCAAAACATCAACAG+TGG	0.653669	3:-40183066	None:intergenic
AAGATCTTGGAGTTCCTAGG+TGG	0.662062	3:+40183233	MsaT013064.1:CDS
CACCTGAAACCGAGCCAGAG+AGG	0.663619	3:-40178582	None:intergenic
TGGTGTGTACTTGTTTACAA+CGG	0.684217	3:-40182886	None:intergenic
GTAGTGATAGGAGTGATGCT+AGG	0.688201	3:-40179809	None:intergenic
GGACATTTAACCCTTCACAA+AGG	0.689149	3:+40178718	MsaT013064.1:CDS
ACTGGAAAACGACGTCGGAG+CGG	0.693740	3:-40180228	None:intergenic
ACAGACGATATATACAGACC+AGG	0.696353	3:+40182362	MsaT013064.1:CDS
TCACGACTGGAAAACGACGT+CGG	0.725346	3:-40180233	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	40178535	40183484	40178535	ID=MsaG013064
Chr3	mRNA	40178535	40183484	40178535	ID=MsaT013064.1;Parent=MsaG013064
Chr3	exon	40178535	40178771	40178535	ID=MsaT013064.1.exon1;Parent=MsaT013064.1
Chr3	CDS	40178535	40178771	40178535	ID=cds.MsaT013064.1;Parent=MsaT013064.1
Chr3	exon	40179359	40179914	40179359	ID=MsaT013064.1.exon2;Parent=MsaT013064.1
Chr3	CDS	40179359	40179914	40179359	ID=cds.MsaT013064.1;Parent=MsaT013064.1
Chr3	exon	40180204	40180279	40180204	ID=MsaT013064.1.exon3;Parent=MsaT013064.1
Chr3	CDS	40180204	40180279	40180204	ID=cds.MsaT013064.1;Parent=MsaT013064.1
Chr3	exon	40180906	40181600	40180906	ID=MsaT013064.1.exon4;Parent=MsaT013064.1
Chr3	CDS	40180906	40181600	40180906	ID=cds.MsaT013064.1;Parent=MsaT013064.1
Chr3	exon	40182232	40182585	40182232	ID=MsaT013064.1.exon5;Parent=MsaT013064.1
Chr3	CDS	40182232	40182585	40182232	ID=cds.MsaT013064.1;Parent=MsaT013064.1
Chr3	exon	40182661	40183484	40182661	ID=MsaT013064.1.exon6;Parent=MsaT013064.1
Chr3	CDS	40182661	40183484	40182661	ID=cds.MsaT013064.1;Parent=MsaT013064.1

Gene Sequence

>MsaT013064.1

ATGGCGGCGGTGAACCCACCGGCATCTGGGAAATCCTTTGCACAAGCCCTCTCTGGCTCGGTTTCAGGTGATTCATTTCTTACTCAGCTTCCTCCGAAGGTGATTGTGGGTGATTCAGTTCGGGTGAAGATCTCTCAAGCAGCATATGAATCTGGTCTTGCAGCTTATCAGTGTAATCTACATGGACATTTAACCCTTCACAAAGGAGATTCACCCTTAGCAACTCAAACACTGAAGGTTGAGGAAGCTATTGTTCACAACATTGCTCAATCTGGAAATGAGGCAGTTGGTAAAAAATCTGCTAAAAATCAAGCTCAATCTAACAATATGGATCCTTTGAATAACTCTGACACTGTTGTGGAAGGTGGTGAGGACCTATTTGTTAAAGTGACCAAAAAGACTGATAACACTAGTTTTGCAAGCTCTGCTATTGTGACAAACCAGAAGGCTGGGAATCCAACAAAACTACCTGTATCCATTTCTTTTGAGTTGTTTGAGAATGATAGTGAGCTTTTTTCAGGGGAGGCTAGGCCAGCTGACATGGAAATTACTCACATTACAGTTGATAAGGTGATGCCTATCAGTCTAAATGTGGAGGACACTTCCCTAGAGAGGGAACACTTGTCTCCTCCTCCATTCTTAGAGGTTCCTAGGGATCCTAATTTTGATGAGTACTTAGTCTTGCAGCCTAGCATCACTCCTATCACTACTACAGATGAAATTCTGGGCCCAGATAAGAGGAAAGTTCAATTCATTGAAGGCACTAAGAATGTGTCAGCTGCAAGCTTAAAAATGAACCTTGCTTCACGTTTGCTCACCGCTCCGACGTCGTTTTCCAGTCGTGATTGCGACGTGATTTCACCGTCTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAATAAGAAATCTGATAAGGTACACTATGTGCGTTATGTTTGTAACAAACAAGGTTTCAAGAAGGGAAGTTTGCTTAATCCTAACAACAGGACAACATCAGACAGTCCATTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCTGAGGAAAGGGTTGGTTGTAAAGCTGGAATATCTTTGAAGTTGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGCTCACTGTCATCCATTACATAAACCAAATCATTCGTGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGCTGGAATGTCGATGAGAACGTCTAATGAAGTTATGGGTCAAGGAGTTGGAGGCACGAAGAATCTTCCTTTTTGGTTTTCAGGCTTAAAGAACTATCTAATGACAATTCGTCAAAAAGAGATGGTGGTTGGTGAAGCAACTATAATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCATCTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGTAGATGGAATTATGCAACTAGATTATTCTCTATTCGGCGATGTCATCAGTTTTGATACAACTTATCGAACAAACAATATATATCGGCCATTAGCTGCCTTTATTGGTTTTGACAATCATCGTAAGAGTGTTTTATTTGGTGTTGCGCTATTGTACGATGAGATAACAACTACTTTTGATTGGTTGTTTAAAACATTCTTGAAGTGTATGTCCAATAAGAAACCACAGACGATATATACAGACCAGGCTAGTGCGTTATTGTGTTCAATTCCGAATAGTTTTCCAGGTGTTTTTCATGGGCTTTGTTCATGGCACATGGCAGAGAATGCAAAGAAAAATCTTGGCTCTCGTGCAAACAATGCATTTTTTGATGAGTTAACTAATTTGGTTTCAAATGTAGACGATGAATCAGATTTTGACTATAATTGGGATCAGATGATGAAAAATTCTTGGGTCAAGTCACATTTTACAGCTGGTCTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCATGGATTTCTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATCTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTCAAGGCTACAAACACTAGAACAAAAAATAATTATCCCAACAGGCAGCTGATGCGGTCCGTTGTAAACAAGTACACACCAGCTTGCTTTGCATTTATTCATAGGGAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACATAACAAAAGGGTCTGCCTTGGAGAAGTTTTTCAAAGTTTTCACAATTGAAAAGCTTGATGATAATTATGATGATGCTGATAATGACGGGCCTTCCACTGTTGATGTTTTGGATGCAGAACTTCAAGAAAACCTTTTCTCAAGTTTTGAAGATCATGATCGACTTGATGAAAGGGTTGTCGCAGTTGACATTAGAAGCAAAAGTTTTAGTTGTTCATGTCATATGTTTGAAAACAGGGGTTCCTTATGTCGACATGTTTTGAAGATCTTGGAGTTCCTAGGTGGTCATGTGCAATATAATTTTTTGAAGACAATACCTAGACAATATGTTATAAAACGTTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCGACCATCCATGTTGGCACTGAAGACACCACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTTTTTGTGCAGATCCGGAGGCATCTAAAATTTTTCTACACGGTTCTTGA

Protein sequence

>MsaT013064.1

MAAVNPPASGKSFAQALSGSVSGDSFLTQLPPKVIVGDSVRVKISQAAYESGLAAYQCNLHGHLTLHKGDSPLATQTLKVEEAIVHNIAQSGNEAVGKKSAKNQAQSNNMDPLNNSDTVVEGGEDLFVKVTKKTDNTSFASSAIVTNQKAGNPTKLPVSISFELFENDSELFSGEARPADMEITHITVDKVMPISLNVEDTSLEREHLSPPPFLEVPRDPNFDEYLVLQPSITPITTTDEILGPDKRKVQFIEGTKNVSAASLKMNLASRLLTAPTSFSSRDCDVISPSRTMLKSKNNSQNKKSDKVHYVRYVCNKQGFKKGSLLNPNNRTTSDSPLVIEFVKVKEKPEERVGCKAGISLKLDEVLNVYKIYRWDVAHCHPLHKPNHSCYLRSFREVNEVQGQLALINSKAGMSMRTSNEVMGQGVGGTKNLPFWFSGLKNYLMTIRQKEMVVGEATIIQEFFRNEALSKPSFYYDIQVDAAEDIASIFWVDGIMQLDYSLFGDVISFDTTYRTNNIYRPLAAFIGFDNHRKSVLFGVALLYDEITTTFDWLFKTFLKCMSNKKPQTIYTDQASALLCSIPNSFPGVFHGLCSWHMAENAKKNLGSRANNAFFDELTNLVSNVDDESDFDYNWDQMMKNSWVKSHFTAGLKTTQLSESFNAFLHGFLQPDHSLVRFFSHLNIMVQRMRDNHAELDFKATNTRTKNNYPNRQLMRSVVNKYTPACFAFIHREYDLSFKYYYEEDITKGSALEKFFKVFTIEKLDDNYDDADNDGPSTVDVLDAELQENLFSSFEDHDRLDERVVAVDIRSKSFSCSCHMFENRGSLCRHVLKILEFLGGHVQYNFLKTIPRQYVIKRWTRDVRPSVDKLKSTIHVGTEDTTQAQRYQQICAVTVQLSTRFCADPEASKIFLHGS*