AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013121


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013121 MtrunA17_Chr3g0092371 92.222 90 2 1 1 90 1 85 8.02e-56 167
MsaG013121 MtrunA17_Chr6g0453221 67.123 73 16 2 1 73 1 65 1.03e-28 98.6
MsaG013121 MtrunA17_Chr4g0060731 68.000 75 21 1 1 75 1 72 8.72e-26 90.9
MsaG013121 MtrunA17_Chr3g0133181 60.294 68 27 0 17 84 72 139 3.76e-24 92.0
MsaG013121 MtrunA17_Chr5g0427051 65.517 58 20 0 23 80 56 113 7.36e-22 85.9
MsaG013121 MtrunA17_Chr1g0188441 59.649 57 23 0 23 79 7 63 6.93e-21 81.6
MsaG013121 MtrunA17_Chr4g0076821 55.405 74 33 0 6 79 94 167 2.65e-20 82.8
MsaG013121 MtrunA17_Chr2g0315941 62.712 59 20 1 16 74 61 117 8.46e-20 80.5
MsaG013121 MtrunA17_Chr5g0412501 61.404 57 22 0 24 80 77 133 1.94e-19 79.3
MsaG013121 MtrunA17_Chr7g0217231 57.143 70 23 2 23 85 86 155 5.87e-19 79.0
MsaG013121 MtrunA17_Chr5g0404271 57.377 61 26 0 18 78 92 152 9.51e-19 78.2
MsaG013121 MtrunA17_Chr8g0367691 62.745 51 19 0 24 74 68 118 2.52e-18 76.6
MsaG013121 MtrunA17_Chr8g0361671 56.667 60 24 1 23 80 62 121 4.81e-17 73.9
MsaG013121 MtrunA17_Chr6g0453231 53.968 63 25 2 25 83 91 153 7.04e-16 70.5
MsaG013121 MtrunA17_Chr6g0479901 53.704 54 24 1 21 73 15 68 1.77e-14 64.7
MsaG013121 MtrunA17_Chr7g0243651 63.415 41 15 0 23 63 1 41 5.01e-12 56.2
MsaG013121 MtrunA17_Chr8g0357721 52.632 38 18 0 34 71 14 51 5.78e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013121 AT1G18835.1 77.273 88 16 2 1 87 1 85 4.52e-41 130
MsaG013121 AT1G74660.1 64.000 100 23 1 1 87 2 101 2.47e-39 126
MsaG013121 AT3G28917.1 63.636 99 26 3 1 89 1 99 6.81e-37 120
MsaG013121 AT1G74660.2 61.702 94 23 1 1 81 2 95 1.63e-33 111
MsaG013121 AT3G50890.1 58.824 68 28 0 8 75 43 110 1.37e-23 90.5
MsaG013121 AT4G24660.1 72.727 55 15 0 23 77 47 101 1.78e-23 89.4
MsaG013121 AT4G24660.2 72.727 55 15 0 23 77 47 101 2.66e-23 89.4
MsaG013121 AT2G02540.2 61.905 63 24 0 23 85 85 147 9.66e-23 89.4
MsaG013121 AT2G02540.1 61.905 63 24 0 23 85 85 147 9.66e-23 89.4
MsaG013121 AT2G18350.1 52.000 75 36 0 10 84 67 141 1.63e-21 85.1
MsaG013121 AT1G14440.1 62.712 59 22 0 23 81 88 146 2.48e-21 85.5
MsaG013121 AT1G14440.2 62.712 59 22 0 23 81 88 146 2.48e-21 85.5
MsaG013121 AT5G65410.1 67.925 53 17 0 23 75 73 125 4.76e-20 81.6
MsaG013121 AT1G75240.1 67.925 53 17 0 23 75 74 126 1.69e-19 80.5
MsaG013121 AT1G69600.1 54.688 64 27 1 25 86 31 94 1.86e-18 76.6
MsaG013121 AT5G15210.1 57.143 63 25 1 25 85 56 118 2.43e-18 77.0
MsaG013121 AT5G42780.1 58.333 60 23 1 19 78 58 115 2.82e-17 73.6
MsaG013121 AT3G28920.1 54.412 68 27 2 23 88 50 115 2.25e-16 72.0
MsaG013121 AT5G60480.1 47.761 67 33 1 23 87 35 101 1.82e-15 68.6
MsaG013121 AT5G39760.1 53.125 64 28 1 25 86 56 119 6.38e-15 68.2
MsaG013121 AT1G14687.1 55.102 49 17 2 25 73 7 50 2.72e-13 62.0

Find 21 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATATCATCGACCATAATT+AGG 0.283610 3:+41186410 None:intergenic
AAGAATCATGCAGCAAATAT+TGG 0.322720 3:-41186578 MsaT013121.1:CDS
ATTACAAGAAATATTAGATA+TGG 0.324448 3:-41186611 MsaT013121.1:CDS
TATTTGCTGCATGATTCTTC+TGG 0.347174 3:+41186582 None:intergenic
GAGTACTCTCCACCTAATTA+TGG 0.393626 3:-41186422 MsaT013121.1:CDS
TGAAAGGATGAAGAAGAGAC+AGG 0.405047 3:-41186672 None:intergenic
GGTATGCTGTAGATGGTTGC+AGG 0.408709 3:-41186553 MsaT013121.1:CDS
AGATGGTTGCAGGGAGTTCA+TGG 0.409911 3:-41186543 MsaT013121.1:CDS
GTGCTGCTTGTGGTTGTCAT+AGG 0.446756 3:-41186484 MsaT013121.1:CDS
ATTGGAGGGTATGCTGTAGA+TGG 0.520125 3:-41186560 MsaT013121.1:CDS
GATGAAGAAGAGACAGGTTG+TGG 0.529455 3:-41186666 MsaT013121.1:CDS
GCTCTTACATGTGCTGCTTG+TGG 0.547604 3:-41186494 MsaT013121.1:CDS
TTCATGGCAAGTACAGAAGA+AGG 0.570746 3:-41186527 MsaT013121.1:CDS
AATCATGCAGCAAATATTGG+AGG 0.579112 3:-41186575 MsaT013121.1:CDS
ATCATGCAGCAAATATTGGA+GGG 0.624158 3:-41186574 MsaT013121.1:CDS
TATCATCGACCATAATTAGG+TGG 0.628535 3:+41186413 None:intergenic
GTATGCTGTAGATGGTTGCA+GGG 0.640365 3:-41186552 MsaT013121.1:CDS
AGTACAGAAGAAGGAACAAG+TGG 0.644860 3:-41186518 MsaT013121.1:CDS
GTACAGAAGAAGGAACAAGT+GGG 0.687829 3:-41186517 MsaT013121.1:CDS
TACAGAAGAAGGAACAAGTG+GGG 0.696853 3:-41186516 MsaT013121.1:CDS
GGTTGTGGTGAAAAGAGATG+TGG 0.713243 3:-41186651 MsaT013121.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 41186415 41186687 41186415 ID=MsaG013121
Chr3 mRNA 41186415 41186687 41186415 ID=MsaT013121.1;Parent=MsaG013121
Chr3 exon 41186415 41186687 41186415 ID=MsaT013121.1.exon1;Parent=MsaT013121.1
Chr3 CDS 41186415 41186687 41186415 ID=cds.MsaT013121.1;Parent=MsaT013121.1
Gene Sequence

>MsaT013121.1

ATGAAGAAGAGACAGGTTGTGGTGAAAAGAGATGTGGCTAAAACATCATCTTCAATTACAAGAAATATTAGATATGGAGAGTGCCAGAAGAATCATGCAGCAAATATTGGAGGGTATGCTGTAGATGGTTGCAGGGAGTTCATGGCAAGTACAGAAGAAGGAACAAGTGGGGCTCTTACATGTGCTGCTTGTGGTTGTCATAGGAACTTTCATAGAAGAGAAGTGCAAACTGAAGTTGTTTGTGAGTACTCTCCACCTAATTATGGTCGATGA

Protein sequence

>MsaT013121.1

MKKRQVVVKRDVAKTSSSITRNIRYGECQKNHAANIGGYAVDGCREFMASTEEGTSGALTCAACGCHRNFHRREVQTEVVCEYSPPNYGR*