Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG013179 | RHN66613.1 | 81.347 | 193 | 29 | 4 | 18 | 204 | 1 | 192 | 1.82e-95 | 286 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG013179 | sp|Q9FKK2|AGL62_ARATH | 31.757 | 148 | 92 | 4 | 53 | 194 | 26 | 170 | 3.80e-15 | 75.5 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG013179 | tr|A0A396IQ06|A0A396IQ06_MEDTR | 81.347 | 193 | 29 | 4 | 18 | 204 | 1 | 192 | 8.68e-96 | 286 |
Gene ID | Type | Classification |
---|---|---|
MsaG013179 | TF | MADS-M-type |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000583 | MsaG013179 | 0.828654 | 1.427985e-54 | 3.977228e-52 |
MsaG003111 | MsaG013179 | 0.842197 | 5.569751e-58 | 2.330706e-55 |
MsaG004186 | MsaG013179 | 0.806399 | 1.421024e-49 | 2.226226e-47 |
MsaG005282 | MsaG013179 | 0.822670 | 3.688917e-53 | 8.710373e-51 |
MsaG005559 | MsaG013179 | 0.802618 | 8.657282e-49 | 1.242139e-46 |
MsaG007094 | MsaG013179 | 0.803591 | 5.457472e-49 | 8.007854e-47 |
MsaG007318 | MsaG013179 | 0.816040 | 1.176860e-51 | 2.335715e-49 |
MsaG009327 | MsaG013179 | 0.808354 | 5.495420e-50 | 9.019360e-48 |
MsaG011049 | MsaG013179 | 0.820456 | 1.191509e-52 | 2.652430e-50 |
MsaG012425 | MsaG013179 | 0.811145 | 1.388937e-50 | 2.439130e-48 |
MsaG013179 | MsaG013177 | 0.878195 | 7.163365e-69 | 1.189616e-65 |
MsaG013179 | MsaG013426 | 0.818887 | 2.707705e-52 | 5.783109e-50 |
MsaG013179 | MsaG013427 | 0.890402 | 2.240544e-73 | 6.743900e-70 |
MsaG013179 | MsaG017163 | 0.802823 | 7.854804e-49 | 1.132275e-46 |
MsaG013179 | MsaG017393 | 0.803573 | 5.504062e-49 | 8.072864e-47 |
MsaG013179 | MsaG017679 | 0.803226 | 6.491441e-49 | 9.445049e-47 |
MsaG013179 | MsaG018669 | 0.840726 | 1.353487e-57 | 5.405221e-55 |
MsaG013179 | MsaG018705 | 0.801373 | 1.555604e-48 | 2.169519e-46 |
MsaG013179 | MsaG020570 | 0.813863 | 3.559816e-51 | 6.687654e-49 |
MsaG013179 | MsaG024181 | 0.808189 | 5.958165e-50 | 9.740109e-48 |
MsaG013179 | MsaG025074 | 0.803245 | 6.432613e-49 | 9.363554e-47 |
MsaG013179 | MsaG027142 | 0.805697 | 1.992804e-49 | 3.070654e-47 |
MsaG013179 | MsaG027329 | 0.808364 | 5.468915e-50 | 8.978017e-48 |
MsaG013179 | MsaG027808 | 0.811732 | 1.037067e-50 | 1.847607e-48 |
MsaG013179 | MsaG027970 | 0.826489 | 4.696486e-54 | 1.231210e-51 |
MsaG013179 | MsaG028180 | 0.818872 | 2.728626e-52 | 5.825525e-50 |
MsaG013179 | MsaG028507 | 0.818104 | 4.067299e-52 | 8.512199e-50 |
MsaG013179 | MsaG029352 | 0.809807 | 2.693208e-50 | 4.577947e-48 |
MsaG013179 | MsaG030113 | 0.819136 | 2.377374e-52 | 5.111152e-50 |
MsaG013179 | MsaG031143 | 0.805722 | 1.969422e-49 | 3.036374e-47 |
MsaG013179 | MsaG032433 | 0.847509 | 2.088004e-59 | 1.040090e-56 |
MsaG013179 | MsaG033125 | 0.822178 | 4.792968e-53 | 1.116825e-50 |
MsaG013179 | MsaG034555 | 0.802941 | 7.429716e-49 | 1.073929e-46 |
MsaG013179 | MsaG040209 | 0.804501 | 3.537298e-49 | 5.301018e-47 |
MsaG013179 | MsaG041706 | 0.867534 | 2.568635e-65 | 2.688195e-62 |
MsaG013179 | MsaG041979 | 0.850027 | 4.214373e-60 | 2.286011e-57 |
MsaG013179 | MsaG041980 | 0.804877 | 2.954203e-49 | 4.466056e-47 |
MsaG013179 | MsaG045800 | 0.814764 | 2.256040e-51 | 4.335266e-49 |
MsaG013179 | MsaG047079 | 0.810899 | 1.569714e-50 | 2.740029e-48 |
MsaG013179 | MsaG007894 | 0.873260 | 3.471473e-67 | 4.625421e-64 |
MsaG013179 | MsaG028085 | 0.800457 | 2.388400e-48 | 3.262815e-46 |
MsaG013179 | MsaG026617 | 0.876706 | 2.351062e-68 | 3.648572e-65 |
MsaG013179 | MsaG027659 | 0.802122 | 1.093926e-48 | 1.551785e-46 |
MsaG013179 | MsaG033538 | 0.814352 | 2.779457e-51 | 5.286096e-49 |
MsaG013179 | MsaG032077 | 0.805812 | 1.886385e-49 | 2.914457e-47 |
MsaG013179 | MsaG037043 | 0.826969 | 3.613023e-54 | 9.598680e-52 |
MsaG013179 | MsaG037187 | 0.825236 | 9.285434e-54 | 2.350912e-51 |
MsaG013179 | MsaG037387 | 0.804282 | 3.926403e-49 | 5.854613e-47 |
MsaG013179 | MsaG047167 | 0.824742 | 1.213219e-53 | 3.030390e-51 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG013179 | MtrunA17_Chr3g0093131 | 81.347 | 193 | 29 | 4 | 18 | 204 | 1 | 192 | 1.67e-99 | 286 |
MsaG013179 | MtrunA17_Chr2g0302091 | 60.396 | 202 | 69 | 7 | 1 | 198 | 2 | 196 | 3.50e-63 | 194 |
MsaG013179 | MtrunA17_Chr8g0345001 | 50.857 | 175 | 73 | 6 | 32 | 197 | 10 | 180 | 3.38e-46 | 150 |
MsaG013179 | MtrunA17_Chr2g0333981 | 36.646 | 161 | 94 | 3 | 31 | 185 | 3 | 161 | 8.98e-25 | 95.5 |
MsaG013179 | MtrunA17_Chr4g0018951 | 36.842 | 133 | 74 | 2 | 53 | 185 | 31 | 153 | 3.10e-22 | 88.6 |
MsaG013179 | MtrunA17_Chr8g0379021 | 34.395 | 157 | 95 | 3 | 35 | 185 | 7 | 161 | 8.10e-20 | 82.8 |
MsaG013179 | MtrunA17_Chr4g0071601 | 33.333 | 147 | 88 | 3 | 48 | 185 | 26 | 171 | 2.41e-18 | 79.0 |
MsaG013179 | MtrunA17_Chr5g0422421 | 36.129 | 155 | 87 | 4 | 38 | 182 | 7 | 159 | 9.19e-18 | 78.6 |
MsaG013179 | MtrunA17_Chr8g0355091 | 30.909 | 165 | 95 | 4 | 31 | 182 | 1 | 159 | 1.00e-15 | 73.2 |
MsaG013179 | MtrunA17_Chr3g0099421 | 32.911 | 158 | 88 | 3 | 38 | 182 | 32 | 184 | 1.57e-15 | 72.8 |
MsaG013179 | MtrunA17_Chr1g0149391 | 32.298 | 161 | 85 | 5 | 38 | 182 | 7 | 159 | 2.01e-15 | 72.4 |
MsaG013179 | MtrunA17_Chr1g0206721 | 31.646 | 158 | 90 | 4 | 38 | 182 | 7 | 159 | 6.41e-15 | 70.9 |
MsaG013179 | MtrunA17_Chr1g0206991 | 32.919 | 161 | 84 | 5 | 38 | 182 | 7 | 159 | 7.29e-15 | 69.7 |
MsaG013179 | MtrunA17_Chr3g0089141 | 33.125 | 160 | 83 | 5 | 39 | 182 | 8 | 159 | 9.39e-15 | 70.1 |
MsaG013179 | MtrunA17_Chr2g0292791 | 31.646 | 158 | 90 | 4 | 38 | 182 | 7 | 159 | 9.82e-15 | 70.5 |
MsaG013179 | MtrunA17_Chr1g0206701 | 31.013 | 158 | 91 | 3 | 38 | 182 | 7 | 159 | 1.16e-14 | 70.1 |
MsaG013179 | MtrunA17_Chr3g0119901 | 29.861 | 144 | 83 | 3 | 53 | 186 | 28 | 163 | 2.10e-14 | 68.9 |
MsaG013179 | MtrunA17_Chr2g0317481 | 29.091 | 165 | 85 | 5 | 38 | 182 | 7 | 159 | 2.26e-14 | 69.3 |
MsaG013179 | MtrunA17_Chr2g0317451 | 29.091 | 165 | 85 | 5 | 38 | 182 | 7 | 159 | 2.47e-14 | 69.3 |
MsaG013179 | MtrunA17_Chr1g0208581 | 33.125 | 160 | 83 | 5 | 39 | 182 | 8 | 159 | 4.88e-14 | 68.2 |
MsaG013179 | MtrunA17_Chr3g0085241 | 26.374 | 182 | 107 | 5 | 15 | 186 | 31 | 195 | 4.96e-14 | 68.6 |
MsaG013179 | MtrunA17_Chr1g0208561 | 32.500 | 160 | 84 | 5 | 39 | 182 | 8 | 159 | 7.63e-14 | 67.8 |
MsaG013179 | MtrunA17_Chr5g0422941 | 29.878 | 164 | 83 | 4 | 38 | 181 | 7 | 158 | 1.45e-13 | 67.0 |
MsaG013179 | MtrunA17_Chr8g0356071 | 42.466 | 73 | 36 | 1 | 41 | 107 | 3 | 75 | 2.41e-13 | 65.1 |
MsaG013179 | MtrunA17_Chr2g0300441 | 33.824 | 136 | 78 | 4 | 54 | 182 | 29 | 159 | 7.40e-12 | 62.4 |
MsaG013179 | MtrunA17_Chr3g0099411 | 43.056 | 72 | 35 | 1 | 38 | 103 | 7 | 78 | 1.27e-11 | 61.6 |
MsaG013179 | MtrunA17_Chr2g0325971 | 31.210 | 157 | 90 | 4 | 39 | 182 | 8 | 159 | 1.31e-11 | 61.6 |
MsaG013179 | MtrunA17_Chr8g0357771 | 46.552 | 58 | 31 | 0 | 53 | 110 | 21 | 78 | 1.81e-11 | 60.5 |
MsaG013179 | MtrunA17_Chr1g0169761 | 41.096 | 73 | 37 | 1 | 41 | 107 | 3 | 75 | 2.94e-11 | 59.7 |
MsaG013179 | MtrunA17_Chr1g0206691 | 30.216 | 139 | 79 | 4 | 53 | 181 | 11 | 141 | 3.27e-11 | 60.5 |
MsaG013179 | MtrunA17_Chr8g0376821 | 42.647 | 68 | 33 | 1 | 41 | 102 | 3 | 70 | 3.83e-11 | 59.7 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG013179 | AT5G60440.1 | 31.757 | 148 | 92 | 4 | 53 | 194 | 26 | 170 | 3.86e-16 | 75.5 |
MsaG013179 | AT2G24840.1 | 28.090 | 178 | 106 | 6 | 25 | 192 | 63 | 228 | 5.25e-16 | 74.7 |
MsaG013179 | AT4G36590.1 | 29.193 | 161 | 96 | 5 | 38 | 182 | 5 | 163 | 1.21e-12 | 65.1 |
MsaG013179 | AT1G65360.1 | 29.747 | 158 | 92 | 5 | 38 | 182 | 5 | 156 | 4.99e-12 | 63.2 |
MsaG013179 | AT3G66656.1 | 51.020 | 49 | 24 | 0 | 53 | 101 | 21 | 69 | 8.49e-12 | 61.6 |
MsaG013179 | AT2G34440.1 | 29.508 | 122 | 80 | 2 | 53 | 171 | 21 | 139 | 8.16e-11 | 58.9 |
Find 31 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TCGCTTCATATCGAAGTCTT+AGG | 0.201474 | 3:+44189308 | None:intergenic |
TAACACACATTCTCTTGAAT+TGG | 0.284587 | 3:+44189082 | None:intergenic |
GAATAAATCTATTGATGATA+TGG | 0.314688 | 3:-44189197 | MsaT013179.1:CDS |
TGAATGACAGCGTCATAGTT+AGG | 0.338378 | 3:+44189400 | None:intergenic |
TGATATTGAATGAAACCAAC+TGG | 0.389424 | 3:-44189651 | MsaT013179.1:CDS |
CGCTTCATATCGAAGTCTTA+GGG | 0.401643 | 3:+44189309 | None:intergenic |
TTGTTTCTCTCGGCCGCCAT+TGG | 0.404729 | 3:+44189586 | None:intergenic |
CTTCCGTGTGTTGTTTCTCT+CGG | 0.413838 | 3:+44189576 | None:intergenic |
CCCTTCTCGAAACTCAAACT+TGG | 0.418484 | 3:-44189520 | MsaT013179.1:CDS |
CTCATCCTCGTCCATATGTC+CGG | 0.445428 | 3:+44189366 | None:intergenic |
CTTATTCATGTTGTCCCAGT+TGG | 0.447593 | 3:+44189636 | None:intergenic |
CTTCTTCGCTTCGCTTGCTA+CGG | 0.451335 | 3:+44189117 | None:intergenic |
CTTCGATATGAAGCGATTCA+AGG | 0.460946 | 3:-44189301 | MsaT013179.1:CDS |
GAAGGATGAAATCAAGAAAG+AGG | 0.477445 | 3:-44189548 | MsaT013179.1:CDS |
GATTTATTCCACCAATCAGC+AGG | 0.485193 | 3:+44189211 | None:intergenic |
GAATGACAGCGTCATAGTTA+GGG | 0.502861 | 3:+44189401 | None:intergenic |
CTCTTCTTCAAGATCATGGA+TGG | 0.520427 | 3:-44189905 | None:intergenic |
CATGCATAGAACTTGTTGTT+GGG | 0.522753 | 3:+44189430 | None:intergenic |
AGGAAAACTTAAAGAAGAGA+AGG | 0.524858 | 3:-44189281 | MsaT013179.1:CDS |
CAACACACGGAAGAGAAAGA+AGG | 0.527351 | 3:-44189566 | MsaT013179.1:CDS |
CCAAGTTTGAGTTTCGAGAA+GGG | 0.530556 | 3:+44189520 | None:intergenic |
TTGATTTGCCTGCTGATTGG+TGG | 0.538450 | 3:-44189219 | MsaT013179.1:CDS |
TGGGACAACATGAATAAGAA+TGG | 0.556319 | 3:-44189631 | MsaT013179.1:CDS |
TCCAAGTTTGAGTTTCGAGA+AGG | 0.565563 | 3:+44189519 | None:intergenic |
ACATGCATAGAACTTGTTGT+TGG | 0.594378 | 3:+44189429 | None:intergenic |
AACAACAAGTTCTATGCATG+TGG | 0.619639 | 3:-44189427 | MsaT013179.1:CDS |
GTCTAAGAAGAAAATTCCAA+TGG | 0.626985 | 3:-44189602 | MsaT013179.1:CDS |
TAAGAAGAAAATTCCAATGG+CGG | 0.679668 | 3:-44189599 | MsaT013179.1:CDS |
CGGCCGAGAGAAACAACACA+CGG | 0.685523 | 3:-44189579 | MsaT013179.1:CDS |
TCTGACCGGACATATGGACG+AGG | 0.696130 | 3:-44189371 | MsaT013179.1:CDS |
GATATTGAATGAAACCAACT+GGG | 0.707052 | 3:-44189650 | MsaT013179.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr3 | gene | 44189081 | 44189912 | 44189081 | ID=MsaG013179 |
Chr3 | mRNA | 44189081 | 44189912 | 44189081 | ID=MsaT013179.1;Parent=MsaG013179 |
Chr3 | exon | 44189081 | 44189688 | 44189081 | ID=MsaT013179.1.exon2;Parent=MsaT013179.1 |
Chr3 | CDS | 44189081 | 44189688 | 44189081 | ID=cds.MsaT013179.1;Parent=MsaT013179.1 |
Chr3 | exon | 44189906 | 44189912 | 44189906 | ID=MsaT013179.1.exon1;Parent=MsaT013179.1 |
Chr3 | CDS | 44189906 | 44189912 | 44189906 | ID=cds.MsaT013179.1;Parent=MsaT013179.1 |
Gene Sequence |
Protein sequence |