AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013179


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013179 MtrunA17_Chr3g0093131 81.347 193 29 4 18 204 1 192 1.67e-99 286
MsaG013179 MtrunA17_Chr2g0302091 60.396 202 69 7 1 198 2 196 3.50e-63 194
MsaG013179 MtrunA17_Chr8g0345001 50.857 175 73 6 32 197 10 180 3.38e-46 150
MsaG013179 MtrunA17_Chr2g0333981 36.646 161 94 3 31 185 3 161 8.98e-25 95.5
MsaG013179 MtrunA17_Chr4g0018951 36.842 133 74 2 53 185 31 153 3.10e-22 88.6
MsaG013179 MtrunA17_Chr8g0379021 34.395 157 95 3 35 185 7 161 8.10e-20 82.8
MsaG013179 MtrunA17_Chr4g0071601 33.333 147 88 3 48 185 26 171 2.41e-18 79.0
MsaG013179 MtrunA17_Chr5g0422421 36.129 155 87 4 38 182 7 159 9.19e-18 78.6
MsaG013179 MtrunA17_Chr8g0355091 30.909 165 95 4 31 182 1 159 1.00e-15 73.2
MsaG013179 MtrunA17_Chr3g0099421 32.911 158 88 3 38 182 32 184 1.57e-15 72.8
MsaG013179 MtrunA17_Chr1g0149391 32.298 161 85 5 38 182 7 159 2.01e-15 72.4
MsaG013179 MtrunA17_Chr1g0206721 31.646 158 90 4 38 182 7 159 6.41e-15 70.9
MsaG013179 MtrunA17_Chr1g0206991 32.919 161 84 5 38 182 7 159 7.29e-15 69.7
MsaG013179 MtrunA17_Chr3g0089141 33.125 160 83 5 39 182 8 159 9.39e-15 70.1
MsaG013179 MtrunA17_Chr2g0292791 31.646 158 90 4 38 182 7 159 9.82e-15 70.5
MsaG013179 MtrunA17_Chr1g0206701 31.013 158 91 3 38 182 7 159 1.16e-14 70.1
MsaG013179 MtrunA17_Chr3g0119901 29.861 144 83 3 53 186 28 163 2.10e-14 68.9
MsaG013179 MtrunA17_Chr2g0317481 29.091 165 85 5 38 182 7 159 2.26e-14 69.3
MsaG013179 MtrunA17_Chr2g0317451 29.091 165 85 5 38 182 7 159 2.47e-14 69.3
MsaG013179 MtrunA17_Chr1g0208581 33.125 160 83 5 39 182 8 159 4.88e-14 68.2
MsaG013179 MtrunA17_Chr3g0085241 26.374 182 107 5 15 186 31 195 4.96e-14 68.6
MsaG013179 MtrunA17_Chr1g0208561 32.500 160 84 5 39 182 8 159 7.63e-14 67.8
MsaG013179 MtrunA17_Chr5g0422941 29.878 164 83 4 38 181 7 158 1.45e-13 67.0
MsaG013179 MtrunA17_Chr8g0356071 42.466 73 36 1 41 107 3 75 2.41e-13 65.1
MsaG013179 MtrunA17_Chr2g0300441 33.824 136 78 4 54 182 29 159 7.40e-12 62.4
MsaG013179 MtrunA17_Chr3g0099411 43.056 72 35 1 38 103 7 78 1.27e-11 61.6
MsaG013179 MtrunA17_Chr2g0325971 31.210 157 90 4 39 182 8 159 1.31e-11 61.6
MsaG013179 MtrunA17_Chr8g0357771 46.552 58 31 0 53 110 21 78 1.81e-11 60.5
MsaG013179 MtrunA17_Chr1g0169761 41.096 73 37 1 41 107 3 75 2.94e-11 59.7
MsaG013179 MtrunA17_Chr1g0206691 30.216 139 79 4 53 181 11 141 3.27e-11 60.5
MsaG013179 MtrunA17_Chr8g0376821 42.647 68 33 1 41 102 3 70 3.83e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013179 AT5G60440.1 31.757 148 92 4 53 194 26 170 3.86e-16 75.5
MsaG013179 AT2G24840.1 28.090 178 106 6 25 192 63 228 5.25e-16 74.7
MsaG013179 AT4G36590.1 29.193 161 96 5 38 182 5 163 1.21e-12 65.1
MsaG013179 AT1G65360.1 29.747 158 92 5 38 182 5 156 4.99e-12 63.2
MsaG013179 AT3G66656.1 51.020 49 24 0 53 101 21 69 8.49e-12 61.6
MsaG013179 AT2G34440.1 29.508 122 80 2 53 171 21 139 8.16e-11 58.9

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTTCATATCGAAGTCTT+AGG 0.201474 3:+44189308 None:intergenic
TAACACACATTCTCTTGAAT+TGG 0.284587 3:+44189082 None:intergenic
GAATAAATCTATTGATGATA+TGG 0.314688 3:-44189197 MsaT013179.1:CDS
TGAATGACAGCGTCATAGTT+AGG 0.338378 3:+44189400 None:intergenic
TGATATTGAATGAAACCAAC+TGG 0.389424 3:-44189651 MsaT013179.1:CDS
CGCTTCATATCGAAGTCTTA+GGG 0.401643 3:+44189309 None:intergenic
TTGTTTCTCTCGGCCGCCAT+TGG 0.404729 3:+44189586 None:intergenic
CTTCCGTGTGTTGTTTCTCT+CGG 0.413838 3:+44189576 None:intergenic
CCCTTCTCGAAACTCAAACT+TGG 0.418484 3:-44189520 MsaT013179.1:CDS
CTCATCCTCGTCCATATGTC+CGG 0.445428 3:+44189366 None:intergenic
CTTATTCATGTTGTCCCAGT+TGG 0.447593 3:+44189636 None:intergenic
CTTCTTCGCTTCGCTTGCTA+CGG 0.451335 3:+44189117 None:intergenic
CTTCGATATGAAGCGATTCA+AGG 0.460946 3:-44189301 MsaT013179.1:CDS
GAAGGATGAAATCAAGAAAG+AGG 0.477445 3:-44189548 MsaT013179.1:CDS
GATTTATTCCACCAATCAGC+AGG 0.485193 3:+44189211 None:intergenic
GAATGACAGCGTCATAGTTA+GGG 0.502861 3:+44189401 None:intergenic
CTCTTCTTCAAGATCATGGA+TGG 0.520427 3:-44189905 None:intergenic
CATGCATAGAACTTGTTGTT+GGG 0.522753 3:+44189430 None:intergenic
AGGAAAACTTAAAGAAGAGA+AGG 0.524858 3:-44189281 MsaT013179.1:CDS
CAACACACGGAAGAGAAAGA+AGG 0.527351 3:-44189566 MsaT013179.1:CDS
CCAAGTTTGAGTTTCGAGAA+GGG 0.530556 3:+44189520 None:intergenic
TTGATTTGCCTGCTGATTGG+TGG 0.538450 3:-44189219 MsaT013179.1:CDS
TGGGACAACATGAATAAGAA+TGG 0.556319 3:-44189631 MsaT013179.1:CDS
TCCAAGTTTGAGTTTCGAGA+AGG 0.565563 3:+44189519 None:intergenic
ACATGCATAGAACTTGTTGT+TGG 0.594378 3:+44189429 None:intergenic
AACAACAAGTTCTATGCATG+TGG 0.619639 3:-44189427 MsaT013179.1:CDS
GTCTAAGAAGAAAATTCCAA+TGG 0.626985 3:-44189602 MsaT013179.1:CDS
TAAGAAGAAAATTCCAATGG+CGG 0.679668 3:-44189599 MsaT013179.1:CDS
CGGCCGAGAGAAACAACACA+CGG 0.685523 3:-44189579 MsaT013179.1:CDS
TCTGACCGGACATATGGACG+AGG 0.696130 3:-44189371 MsaT013179.1:CDS
GATATTGAATGAAACCAACT+GGG 0.707052 3:-44189650 MsaT013179.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 44189081 44189912 44189081 ID=MsaG013179
Chr3 mRNA 44189081 44189912 44189081 ID=MsaT013179.1;Parent=MsaG013179
Chr3 exon 44189081 44189688 44189081 ID=MsaT013179.1.exon2;Parent=MsaT013179.1
Chr3 CDS 44189081 44189688 44189081 ID=cds.MsaT013179.1;Parent=MsaT013179.1
Chr3 exon 44189906 44189912 44189906 ID=MsaT013179.1.exon1;Parent=MsaT013179.1
Chr3 CDS 44189906 44189912 44189906 ID=cds.MsaT013179.1;Parent=MsaT013179.1
Gene Sequence

>MsaT013179.1

ATGGATGATAATTCTTCATCAGTGATATTGAATGAAACCAACTGGGACAACATGAATAAGAATGGTGAAACGTCTAAGAAGAAAATTCCAATGGCGGCCGAGAGAAACAACACACGGAAGAGAAAGAAGGATGAAATCAAGAAAGAGGAAAAGCCCTTCTCGAAACTCAAACTTGGAATTTTCAACAAAGCAATAGAGCTATCTATTCTATGCGAATCAGAAACTGCACTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAACTATGACGCTGTCATTCATCGTTTTCTGACCGGACATATGGACGAGGATGAGAAAAAGAAGCAAGAAGATGATGACGTTGTTGAAACCCTAAGACTTCGATATGAAGCGATTCAAGGAAAACTTAAAGAAGAGAAGGAGAAATTGCAAGCTGTCAAAGATGCACACAAGAGTAATTTTGATTTGCCTGCTGATTGGTGGAATAAATCTATTGATGATATGGATTTAACATCTCTTGAAGATATTACGACTTCGTTGAATAAATTGAAGATTAATTTGTCCGTAGCAAGCGAAGCGAAGAAGTTCAATTTACATCCAATTCAAGAGAATGTGTGTTAA

Protein sequence

>MsaT013179.1

MDDNSSSVILNETNWDNMNKNGETSKKKIPMAAERNNTRKRKKDEIKKEEKPFSKLKLGIFNKAIELSILCESETALIVTSPNNKFYACGYPNYDAVIHRFLTGHMDEDEKKKQEDDDVVETLRLRYEAIQGKLKEEKEKLQAVKDAHKSNFDLPADWWNKSIDDMDLTSLEDITTSLNKLKINLSVASEAKKFNLHPIQENVC*