Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013249 | KEH26852.1 | 47.561 | 82 | 43 | 0 | 29 | 110 | 65 | 146 | 1.28e-16 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013249 | tr|A0A072UB72|A0A072UB72_MEDTR | 47.561 | 82 | 43 | 0 | 29 | 110 | 65 | 146 | 6.09e-17 | 84.3 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG013249 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013249 | MtrunA17_Chr2g0281511 | 30.108 | 93 | 65 | 0 | 16 | 108 | 27 | 119 | 6.73e-13 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGTTGATGGAGATCATATT+AGG | 0.276691 | 3:-45749118 | None:intergenic |
| CTTGAACCTATTGAATTTAC+CGG | 0.312243 | 3:+45747931 | MsaT013249.1:CDS |
| TCTAGTTTGCAATATTGTTT+CGG | 0.367587 | 3:-45749351 | None:intergenic |
| AATTTACCGGTAAATTCAAT+AGG | 0.369371 | 3:-45747937 | None:intergenic |
| GCAAGCTTATCACTTCAAAC+AGG | 0.387345 | 3:+45749203 | MsaT013249.1:CDS |
| ACACCATTACATTAGTGAAT+TGG | 0.388195 | 3:+45749158 | MsaT013249.1:CDS |
| GATCTCCATCAACGTACTTC+CGG | 0.393292 | 3:+45749126 | MsaT013249.1:CDS |
| CGTTGATGGAGATCATATTA+GGG | 0.420134 | 3:-45749117 | None:intergenic |
| TGTATCCGGAAGTACGTTGA+TGG | 0.473916 | 3:-45749131 | None:intergenic |
| ATTCCAATTCACTAATGTAA+TGG | 0.506865 | 3:-45749161 | None:intergenic |
| AAGACAACAAATTGCGTCAA+AGG | 0.508128 | 3:+45749272 | MsaT013249.1:CDS |
| GTAATGGTGTGCTGTGTATC+CGG | 0.513065 | 3:-45749145 | None:intergenic |
| AGACAACAAATTGCGTCAAA+GGG | 0.524945 | 3:+45749273 | MsaT013249.1:CDS |
| ACAAATTGCGTCAAAGGGAT+AGG | 0.525425 | 3:+45749278 | MsaT013249.1:CDS |
| TTAATAGTTATGTCTTCAGA+AGG | 0.538888 | 3:+45747881 | MsaT013249.1:CDS |
| ATCTCAACAAATAATGACTT+CGG | 0.550533 | 3:-45749325 | None:intergenic |
| CAAGCTTATCACTTCAAACA+GGG | 0.577453 | 3:+45749204 | MsaT013249.1:CDS |
| AGACATAACTATTAAGCAAG+AGG | 0.584867 | 3:-45747873 | None:intergenic |
| CAACAAATAATGACTTCGGA+GGG | 0.599576 | 3:-45749321 | None:intergenic |
| TCAACAAATAATGACTTCGG+AGG | 0.612423 | 3:-45749322 | None:intergenic |
| CAATAGGTTCAAGATAGTTG+TGG | 0.613285 | 3:-45747921 | None:intergenic |
| AAGCTTATCACTTCAAACAG+GGG | 0.733630 | 3:+45749205 | MsaT013249.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 45747869 | 45749372 | 45747869 | ID=MsaG013249 |
| Chr3 | mRNA | 45747869 | 45749372 | 45747869 | ID=MsaT013249.1;Parent=MsaG013249 |
| Chr3 | exon | 45747869 | 45747952 | 45747869 | ID=MsaT013249.1.exon1;Parent=MsaT013249.1 |
| Chr3 | CDS | 45747869 | 45747952 | 45747869 | ID=cds.MsaT013249.1;Parent=MsaT013249.1 |
| Chr3 | exon | 45749112 | 45749372 | 45749112 | ID=MsaT013249.1.exon2;Parent=MsaT013249.1 |
| Chr3 | CDS | 45749112 | 45749372 | 45749112 | ID=cds.MsaT013249.1;Parent=MsaT013249.1 |
| Gene Sequence |
| Protein sequence |
