Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| MsaG013509 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGAACGTGGCTAATTTC+TGG | 0.218063 | 3:+55165404 | MsaT013509.1:CDS |
| TGTTCTTCCACCACTGGTAC+TGG | 0.276664 | 3:-55165349 | None:intergenic |
| AATTAGCCACGTTCTGAATC+TGG | 0.296437 | 3:-55165399 | None:intergenic |
| GAGGAACCTGCTTTCGATAT+TGG | 0.318864 | 3:+55165436 | MsaT013509.1:CDS |
| AAATTTGCTCGGCGAGCATT+TGG | 0.329497 | 3:+55165717 | MsaT013509.1:CDS |
| TGATAATGAGGAGGCTGATA+TGG | 0.331856 | 3:+55165459 | MsaT013509.1:CDS |
| TTAAAACATGTGTCTCCAAC+TGG | 0.380379 | 3:-55165528 | None:intergenic |
| GCACGTGAGGCAGGTCGTTA+TGG | 0.400336 | 3:+55165938 | MsaT013509.1:CDS |
| AATACCGATGTAGCTTTATC+CGG | 0.408209 | 3:-55165819 | None:intergenic |
| TCAGAACGTGGCTAATTTCT+GGG | 0.416922 | 3:+55165405 | MsaT013509.1:CDS |
| GCTACTGCAGCGGCAGTTTG+TGG | 0.430209 | 3:-55165376 | None:intergenic |
| AATTTGCTCGGCGAGCATTT+GGG | 0.441935 | 3:+55165718 | MsaT013509.1:CDS |
| GCCTCACGTGCAGGACAGTT+GGG | 0.444919 | 3:-55165926 | None:intergenic |
| TCATTCCACTTAATATCGAT+TGG | 0.456651 | 3:-55165792 | None:intergenic |
| CGTTAGAGAGAACAGATTTG+TGG | 0.458615 | 3:+55165866 | MsaT013509.1:CDS |
| TCCACCACTGGTACTGGGTC+AGG | 0.460719 | 3:-55165343 | None:intergenic |
| GTTCTTCCACCACTGGTACT+GGG | 0.464509 | 3:-55165348 | None:intergenic |
| TGCCTCACGTGCAGGACAGT+TGG | 0.476556 | 3:-55165927 | None:intergenic |
| AGTATCCAATCGATATTAAG+TGG | 0.481943 | 3:+55165787 | MsaT013509.1:CDS |
| CTCATCTCGGATCGTGATGA+AGG | 0.492063 | 3:-55165754 | None:intergenic |
| GGCTGATATGGATGTCGAAA+TGG | 0.496866 | 3:+55165471 | MsaT013509.1:CDS |
| AACTGTCCTGCACGTGAGGC+AGG | 0.503776 | 3:+55165929 | MsaT013509.1:CDS |
| ATTAGCCACGTTCTGAATCT+GGG | 0.515846 | 3:-55165398 | None:intergenic |
| TTTCGATATTGGTGATAATG+AGG | 0.526872 | 3:+55165447 | MsaT013509.1:CDS |
| TTTAACGCTTGCTGCGTACA+TGG | 0.531915 | 3:+55165546 | MsaT013509.1:CDS |
| TAACGACCTGCCTCACGTGC+AGG | 0.537930 | 3:-55165935 | None:intergenic |
| TCTCGGATCGTGATGAAGGT+TGG | 0.548455 | 3:-55165750 | None:intergenic |
| TAAAACATGTGTCTCCAACT+GGG | 0.552768 | 3:-55165527 | None:intergenic |
| TTGTACCAGCAAAATTTGCT+CGG | 0.557897 | 3:+55165706 | MsaT013509.1:CDS |
| GGCAGGTCGTTATGGTGTAA+AGG | 0.566786 | 3:+55165946 | MsaT013509.1:CDS |
| ACAGATTTGTGGAAGGCACA+AGG | 0.581028 | 3:+55165877 | MsaT013509.1:CDS |
| TTATCACCAATATCGAAAGC+AGG | 0.592587 | 3:-55165442 | None:intergenic |
| ACCCAACTGTCCTGCACGTG+AGG | 0.595873 | 3:+55165925 | MsaT013509.1:CDS |
| TGCACATGGCAATCCCCAGT+TGG | 0.598828 | 3:+55165513 | MsaT013509.1:CDS |
| AAAACATGTGTCTCCAACTG+GGG | 0.599659 | 3:-55165526 | None:intergenic |
| CGATATTGGTGATAATGAGG+AGG | 0.613156 | 3:+55165450 | MsaT013509.1:CDS |
| TGTTCCTGACCCAGTACCAG+TGG | 0.613436 | 3:+55165339 | MsaT013509.1:CDS |
| CAGATTTGTGGAAGGCACAA+GGG | 0.620094 | 3:+55165878 | MsaT013509.1:CDS |
| CTGAATCTGGGCTACTGCAG+CGG | 0.621166 | 3:-55165386 | None:intergenic |
| ACTCACGGTGGAACTCATCT+CGG | 0.624872 | 3:-55165767 | None:intergenic |
| TCCTGACCCAGTACCAGTGG+TGG | 0.629390 | 3:+55165342 | MsaT013509.1:CDS |
| TAATATCGATTGGATACTCA+CGG | 0.630661 | 3:-55165782 | None:intergenic |
| AGAGAGAACAGATTTGTGGA+AGG | 0.635968 | 3:+55165870 | MsaT013509.1:CDS |
| TAATTTCTGGGATGATGATG+AGG | 0.648095 | 3:+55165417 | MsaT013509.1:CDS |
| TATGGATGTCGAAATGGATG+TGG | 0.657183 | 3:+55165477 | MsaT013509.1:CDS |
| AAAACCGGATAAAGCTACAT+CGG | 0.665649 | 3:+55165815 | MsaT013509.1:CDS |
| CATGGTGAAATGCAGCAACA+TGG | 0.687154 | 3:+55165564 | MsaT013509.1:CDS |
| AATGGATGTGGTAGATGCTG+AGG | 0.688566 | 3:+55165489 | MsaT013509.1:CDS |
| AGTAGCCCAGATTCAGAACG+TGG | 0.698194 | 3:+55165393 | MsaT013509.1:CDS |
| GTAGATGCTGAGGATGCACA+TGG | 0.739903 | 3:+55165499 | MsaT013509.1:CDS |
| TATCGATTGGATACTCACGG+TGG | 0.816467 | 3:-55165779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 55165331 | 55165965 | 55165331 | ID=MsaG013509 |
| Chr3 | mRNA | 55165331 | 55165965 | 55165331 | ID=MsaT013509.1;Parent=MsaG013509 |
| Chr3 | exon | 55165331 | 55165585 | 55165331 | ID=MsaT013509.1.exon1;Parent=MsaT013509.1 |
| Chr3 | CDS | 55165331 | 55165585 | 55165331 | ID=cds.MsaT013509.1;Parent=MsaT013509.1 |
| Chr3 | exon | 55165705 | 55165965 | 55165705 | ID=MsaT013509.1.exon2;Parent=MsaT013509.1 |
| Chr3 | CDS | 55165705 | 55165965 | 55165705 | ID=cds.MsaT013509.1;Parent=MsaT013509.1 |
| Gene Sequence |
| Protein sequence |
