AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013595


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013595 MtrunA17_Chr3g0097221 91.554 296 19 3 1 295 1 291 0.0 503
MsaG013595 MtrunA17_Chr5g0402611 63.529 170 50 5 2 169 3 162 8.05e-61 196
MsaG013595 MtrunA17_Chr3g0115431 74.576 118 28 2 1 117 2 118 2.00e-55 179
MsaG013595 MtrunA17_Chr4g0058651 67.593 108 35 0 5 112 9 116 3.47e-48 159
MsaG013595 MtrunA17_Chr7g0227511 67.290 107 35 0 4 110 8 114 2.96e-47 156
MsaG013595 MtrunA17_Chr6g0449641 65.455 110 38 0 2 111 4 113 4.85e-47 154
MsaG013595 MtrunA17_Chr3g0117121 65.179 112 39 0 2 113 5 116 5.60e-47 156
MsaG013595 MtrunA17_Chr8g0353981 69.091 110 34 0 1 110 1 110 4.96e-46 155
MsaG013595 MtrunA17_Chr5g0437571 50.000 148 61 3 5 149 4 141 1.10e-44 149
MsaG013595 MtrunA17_Chr6g0449651 63.551 107 39 0 4 110 64 170 7.16e-44 148
MsaG013595 MtrunA17_Chr3g0114061 45.695 151 71 2 3 152 4 144 5.02e-42 142
MsaG013595 MtrunA17_Chr3g0106101 55.140 107 48 0 1 107 1 107 6.96e-41 140
MsaG013595 MtrunA17_Chr8g0374601 39.070 215 105 5 7 216 26 219 3.77e-40 141
MsaG013595 MtrunA17_Chr4g0043421 60.396 101 40 0 6 106 14 114 7.20e-40 137
MsaG013595 MtrunA17_Chr5g0438091 55.963 109 48 0 4 112 36 144 8.76e-40 137
MsaG013595 MtrunA17_Chr3g0129071 57.009 107 46 0 6 112 46 152 2.68e-39 137
MsaG013595 MtrunA17_Chr2g0311601 54.630 108 49 0 6 113 12 119 9.11e-39 134
MsaG013595 MtrunA17_Chr3g0113891 58.824 102 42 0 4 105 11 112 2.09e-38 134
MsaG013595 MtrunA17_Chr5g0437401 56.190 105 46 0 4 108 8 112 8.18e-38 132
MsaG013595 MtrunA17_Chr1g0184281 59.048 105 42 1 3 106 11 115 5.40e-36 128
MsaG013595 MtrunA17_Chr8g0374591 53.571 112 51 1 7 118 8 118 2.66e-34 121
MsaG013595 MtrunA17_Chr7g0260971 53.448 116 46 2 1 108 1 116 4.10e-34 123
MsaG013595 MtrunA17_Chr1g0200201 52.885 104 49 0 8 111 73 176 5.05e-34 124
MsaG013595 MtrunA17_Chr4g0030001 45.455 132 69 2 6 136 23 152 7.39e-34 122
MsaG013595 MtrunA17_Chr5g0432451 56.604 106 46 0 6 111 42 147 7.75e-34 123
MsaG013595 MtrunA17_Chr8g0374581 37.339 233 110 7 7 235 8 208 8.91e-34 123
MsaG013595 MtrunA17_Chr5g0444351 43.357 143 76 2 8 148 6 145 1.46e-33 120
MsaG013595 MtrunA17_Chr7g0225111 49.091 110 56 0 6 115 4 113 2.18e-33 118
MsaG013595 MtrunA17_Chr7g0261031 50.877 114 55 1 1 113 2 115 2.50e-32 119
MsaG013595 MtrunA17_Chr8g0351701 52.336 107 50 1 7 112 23 129 9.94e-32 117
MsaG013595 MtrunA17_Chr7g0245871 55.882 102 44 1 7 107 27 128 4.18e-31 116
MsaG013595 MtrunA17_Chr5g0435641 37.126 167 90 5 1 159 1 160 9.47e-31 114
MsaG013595 MtrunA17_Chr1g0184271 45.385 130 69 2 7 134 12 141 1.04e-30 115
MsaG013595 MtrunA17_Chr8g0351721 50.893 112 54 1 7 117 18 129 5.25e-30 112
MsaG013595 MtrunA17_Chr6g0460951 41.333 150 78 3 6 152 39 181 4.36e-29 110
MsaG013595 MtrunA17_Chr6g0452691 33.511 188 104 3 1 187 3 170 1.14e-28 108
MsaG013595 MtrunA17_Chr7g0245861 56.667 90 36 2 7 93 12 101 5.72e-25 97.4
MsaG013595 MtrunA17_Chr6g0460941 45.263 95 51 1 1 94 1 95 8.70e-25 95.5
MsaG013595 MtrunA17_Chr6g0452661 43.925 107 55 2 5 108 7 111 2.73e-24 98.6
MsaG013595 MtrunA17_Chr6g0452671 41.584 101 59 0 4 104 8 108 1.13e-23 95.9
MsaG013595 MtrunA17_Chr4g0055441 41.176 102 59 1 8 108 3 104 1.23e-23 94.4
MsaG013595 MtrunA17_Chr4g0059901 45.000 100 55 0 8 107 11 110 5.13e-23 95.5
MsaG013595 MtrunA17_Chr8g0342351 37.864 103 64 0 8 110 13 115 4.18e-21 90.1
MsaG013595 MtrunA17_Chr5g0439111 43.519 108 60 1 5 111 7 114 4.84e-21 89.4
MsaG013595 MtrunA17_Chr4g0040831 39.175 97 59 0 8 104 16 112 2.62e-18 81.3
MsaG013595 MtrunA17_Chr2g0326041 60.417 48 19 0 6 53 32 79 9.13e-17 73.9
MsaG013595 MtrunA17_Chr2g0330201 33.663 101 67 0 8 108 20 120 2.14e-16 77.8
MsaG013595 MtrunA17_Chr4g0046201 34.409 93 54 1 8 100 7 92 8.46e-14 70.1
MsaG013595 MtrunA17_Chr2g0326051 41.667 84 46 1 52 132 5 88 1.34e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013595 AT5G66870.1 64.925 134 46 1 1 134 1 133 7.94e-54 178
MsaG013595 AT2G23660.2 68.224 107 34 0 4 110 2 108 5.56e-49 165
MsaG013595 AT2G23660.3 68.224 107 34 0 4 110 2 108 5.56e-49 165
MsaG013595 AT2G23660.1 68.224 107 34 0 4 110 2 108 5.56e-49 165
MsaG013595 AT5G63090.4 65.179 112 39 0 2 113 6 117 7.80e-48 158
MsaG013595 AT5G63090.3 65.179 112 39 0 2 113 6 117 7.80e-48 158
MsaG013595 AT5G63090.2 65.179 112 39 0 2 113 6 117 7.80e-48 158
MsaG013595 AT5G63090.1 65.179 112 39 0 2 113 6 117 7.80e-48 158
MsaG013595 AT3G27650.1 65.421 107 37 0 4 110 36 142 6.19e-46 152
MsaG013595 AT1G65620.1 69.725 109 33 0 2 110 4 112 4.58e-45 152
MsaG013595 AT1G65620.4 69.725 109 33 0 2 110 4 112 4.58e-45 152
MsaG013595 AT1G65620.3 69.725 109 33 0 2 110 4 112 4.58e-45 152
MsaG013595 AT1G65620.5 69.725 109 33 0 2 110 4 112 4.58e-45 152
MsaG013595 AT1G65620.2 69.725 109 33 0 2 110 4 112 4.58e-45 152
MsaG013595 AT2G30130.1 54.783 115 51 1 5 118 6 120 2.11e-41 142
MsaG013595 AT1G31320.1 65.909 88 30 0 6 93 12 99 3.01e-38 133
MsaG013595 AT1G07900.1 49.242 132 62 1 6 137 32 158 6.13e-38 133
MsaG013595 AT3G26660.1 53.465 101 47 0 8 108 6 106 2.32e-37 129
MsaG013595 AT3G26620.1 53.465 101 47 0 8 108 6 106 2.70e-37 129
MsaG013595 AT2G28500.1 45.695 151 77 3 6 151 54 204 3.74e-37 132
MsaG013595 AT3G11090.1 55.556 108 48 0 4 111 8 115 5.83e-37 129
MsaG013595 AT2G30340.2 50.000 118 59 0 6 123 52 169 8.32e-37 132
MsaG013595 AT2G30340.1 50.000 118 59 0 6 123 51 168 9.92e-37 132
MsaG013595 AT2G40470.2 57.143 105 45 0 6 110 19 123 4.35e-36 128
MsaG013595 AT2G40470.1 57.143 105 45 0 6 110 44 148 1.35e-35 128
MsaG013595 AT1G16530.1 57.282 103 43 1 6 107 13 115 4.71e-35 125
MsaG013595 AT2G42430.1 53.774 106 48 1 6 110 14 119 4.28e-34 124
MsaG013595 AT3G03760.1 55.340 103 45 1 6 107 50 152 4.78e-33 122
MsaG013595 AT3G58190.1 51.456 103 49 1 7 108 11 113 9.68e-32 117
MsaG013595 AT2G42440.1 49.533 107 53 1 1 106 1 107 6.27e-31 116
MsaG013595 AT3G50510.1 40.876 137 76 1 1 132 6 142 8.83e-31 114
MsaG013595 AT3G50510.2 40.876 137 76 1 1 132 6 142 8.83e-31 114
MsaG013595 AT2G31310.1 43.478 138 76 2 6 142 6 142 1.72e-30 113
MsaG013595 AT2G45420.1 52.885 104 48 1 10 112 40 143 3.39e-29 112
MsaG013595 AT4G00210.1 52.427 103 48 1 7 108 11 113 3.63e-29 111
MsaG013595 AT4G00220.1 51.923 104 49 1 10 112 20 123 5.04e-29 110
MsaG013595 AT5G06080.1 45.690 116 62 1 1 115 1 116 1.66e-27 105
MsaG013595 AT2G45410.1 50.000 114 52 2 10 118 19 132 8.49e-27 103
MsaG013595 AT3G13850.1 39.806 103 62 0 8 110 37 139 2.16e-24 99.8
MsaG013595 AT5G35900.1 43.137 102 58 0 8 109 6 107 3.22e-24 97.8
MsaG013595 AT3G47870.1 39.806 103 62 0 8 110 37 139 6.93e-23 96.7
MsaG013595 AT1G72980.1 37.500 104 65 0 4 107 10 113 8.95e-19 83.2
MsaG013595 AT1G06280.1 36.364 110 68 1 8 117 25 132 4.41e-17 78.6
MsaG013595 AT4G22700.1 36.538 104 64 2 8 110 6 108 2.09e-15 73.6
MsaG013595 AT2G19820.1 34.066 91 59 1 4 93 9 99 8.24e-13 64.7
MsaG013595 AT5G15060.1 42.466 73 40 1 8 78 18 90 4.90e-12 62.8

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATGGAATTCGCAGTTTC+CGG 0.214379 3:+57010508 MsaT013595.1:CDS
TGCTGATGTGGCTCATCCTT+TGG 0.240291 3:+57010745 MsaT013595.1:CDS
CAGTGCTTGTTGATGAATTC+TGG 0.277883 3:+57010449 MsaT013595.1:CDS
GTGTCGCCGCCGCCGCCGGC+GGG 0.284860 3:+57010801 MsaT013595.1:CDS
ATCTCTTGAGGAAAATTGTT+AGG 0.328058 3:-57010545 None:intergenic
GTCGCCGCCGCCGCCGGCGG+GGG 0.357846 3:+57010803 MsaT013595.1:CDS
CATCAGATGGGTGCTGCTGC+TGG 0.359121 3:+57010701 MsaT013595.1:CDS
GCTGATGTGGCTCATCCTTT+GGG 0.359353 3:+57010746 MsaT013595.1:CDS
CGTATAAAAGACCCTGTCTA+TGG 0.366420 3:+57010317 MsaT013595.1:CDS
CAATGGAATTCGCAGTTTCC+GGG 0.387871 3:+57010509 MsaT013595.1:CDS
AACAAAAGCTGTGCCTCAAC+TGG 0.391954 3:-57010896 None:intergenic
TGAAGACGCGGTGAAATCTT+TGG 0.393603 3:+57010277 MsaT013595.1:CDS
TGCTGCTGCTGTTGCTGCTG+AGG 0.396294 3:-57010485 None:intergenic
GGTGTCGCCGCCGCCGCCGG+CGG 0.400660 3:+57010800 MsaT013595.1:CDS
GGTGGTGTCGCCGCCGCCGC+CGG 0.400920 3:+57010797 MsaT013595.1:CDS
GCTAATGTTCACCGTGTCTT+CGG 0.403676 3:+57010206 MsaT013595.1:CDS
TTGAGGATTCAAATTATAAC+CGG 0.410153 3:-57010420 None:intergenic
GAGTTTCTCTTAACTTCTGT+TGG 0.444328 3:-57010364 None:intergenic
CAAGAGTATTTGCTTAGGTC+TGG 0.445183 3:+57010647 MsaT013595.1:CDS
AAGATTTCACCGCGTCTTCA+CGG 0.446492 3:-57010274 None:intergenic
TGTCGCCGCCGCCGCCGGCG+GGG 0.448315 3:+57010802 MsaT013595.1:CDS
CTTAGGTCTGGTACTGGTGG+TGG 0.448584 3:+57010659 MsaT013595.1:CDS
AGCAACAGCAGCAGCAGCAA+TGG 0.455503 3:+57010492 MsaT013595.1:CDS
AACAAGCACTGCATGGTTTG+AGG 0.465438 3:-57010437 None:intergenic
CCTCAAGAGATGGTTGGAGC+AGG 0.471809 3:+57010557 MsaT013595.1:CDS
TGTGGCTCATCCTTTGGGCA+TGG 0.493594 3:+57010751 MsaT013595.1:CDS
TCTCTTGAGGAAAATTGTTA+GGG 0.494095 3:-57010544 None:intergenic
GGTGGATATCAGATGGGTGG+TGG 0.514995 3:+57010677 MsaT013595.1:CDS
TACTGGTGGTGGATATCAGA+TGG 0.516614 3:+57010670 MsaT013595.1:CDS
TATTTGCTTAGGTCTGGTAC+TGG 0.525978 3:+57010653 MsaT013595.1:CDS
CAAGAGATGGTTGGAGCAGG+TGG 0.526908 3:+57010560 MsaT013595.1:CDS
AGACCTACACATCCATAGAC+AGG 0.526929 3:-57010329 None:intergenic
CTCCACAAGAGTATTTGCTT+AGG 0.529751 3:+57010642 MsaT013595.1:CDS
TCACCAGCTTCTTGATTCTG+TGG 0.531207 3:-57010923 None:intergenic
GATGGGTGGTGGACATCAGA+TGG 0.534956 3:+57010688 MsaT013595.1:CDS
TGCTGCACATGGTGAATTTG+AGG 0.537271 3:-57010105 None:intergenic
CAGATGGGTGCTGCTGCTGG+TGG 0.542743 3:+57010704 MsaT013595.1:CDS
AATCAAGAAGCTGGTGAGGA+TGG 0.547877 3:+57010929 MsaT013595.1:CDS
TGTTGCTCTCCTCCTTGCTG+TGG 0.554043 3:-57010839 None:intergenic
GTGGCTCATCCTTTGGGCAT+GGG 0.556217 3:+57010752 MsaT013595.1:CDS
TGTTTCTGATCATGCTGATG+TGG 0.557271 3:+57010733 MsaT013595.1:CDS
CATGACCTTCAGATGGGTGG+TGG 0.558716 3:+57010608 MsaT013595.1:CDS
TCTCCACAGAATCAAGAAGC+TGG 0.567957 3:+57010920 MsaT013595.1:CDS
TTGCTTAGGTCTGGTACTGG+TGG 0.569078 3:+57010656 MsaT013595.1:CDS
CGTTGCTTGCACCGAAGACA+CGG 0.571833 3:-57010217 None:intergenic
TCAAAATTTCCCATGCCCAA+AGG 0.572126 3:-57010761 None:intergenic
TTAGGGTAATTCACAAAACC+CGG 0.573567 3:-57010527 None:intergenic
CAACAACATAATTATCATGT+TGG 0.574402 3:+57010857 MsaT013595.1:CDS
ATTGAAAGAGCTTGACGTGA+AGG 0.574685 3:+57010250 MsaT013595.1:CDS
ACAAGAACATGACCTTCAGA+TGG 0.577454 3:+57010601 MsaT013595.1:CDS
GACCTAAGCAAATACTCTTG+TGG 0.580143 3:-57010644 None:intergenic
ACTGGTGGTGGATATCAGAT+GGG 0.581105 3:+57010671 MsaT013595.1:CDS
TATCATGTTGGTGTTGCTCA+TGG 0.587574 3:+57010869 MsaT013595.1:CDS
AGAAACTTGCATGCTGCACA+TGG 0.594029 3:-57010116 None:intergenic
GACCCTGTCTATGGATGTGT+AGG 0.599058 3:+57010326 MsaT013595.1:CDS
ATGGGTGGTGGACATCAGAT+GGG 0.608197 3:+57010689 MsaT013595.1:CDS
GATATCCACCACCCATCTGA+AGG 0.610464 3:-57010613 None:intergenic
ATTCATCAACAAGCACTGCA+TGG 0.613761 3:-57010444 None:intergenic
ACAGAATCAAGAAGCTGGTG+AGG 0.614291 3:+57010925 MsaT013595.1:CDS
CAAGAACATGACCTTCAGAT+GGG 0.623978 3:+57010602 MsaT013595.1:CDS
TGCTCATGGTAATCCAGTTG+AGG 0.628804 3:+57010883 MsaT013595.1:CDS
GGTGAGGATGGTAAAAGTGT+TGG 0.628808 3:+57010941 MsaT013595.1:CDS
GGTGAAATCTTTGGCATACG+AGG 0.628886 3:+57010286 MsaT013595.1:CDS
GGTGGTGGATATCAGATGGG+TGG 0.634173 3:+57010674 MsaT013595.1:CDS
CGTGAAGGACCGTGAAGACG+CGG 0.637430 3:+57010265 MsaT013595.1:CDS
TGAACATTAGCGAATCGCTG+TGG 0.642216 3:-57010194 None:intergenic
CCTGCTCCAACCATCTCTTG+AGG 0.644914 3:-57010557 None:intergenic
GAACATGACCTTCAGATGGG+TGG 0.648756 3:+57010605 MsaT013595.1:CDS
TATTATCAGATTCCACAGCA+AGG 0.670948 3:+57010827 MsaT013595.1:CDS
TTTGACAATGGTGCTACTGG+TGG 0.683253 3:+57010779 MsaT013595.1:CDS
GACCTACACATCCATAGACA+GGG 0.696270 3:-57010328 None:intergenic
AACTCAAAATGAACTCACTG+TGG 0.711640 3:+57010382 MsaT013595.1:CDS
TATCAGATTCCACAGCAAGG+AGG 0.712123 3:+57010830 MsaT013595.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 57010098 57010985 57010098 ID=MsaG013595
Chr3 mRNA 57010098 57010985 57010098 ID=MsaT013595.1;Parent=MsaG013595
Chr3 exon 57010098 57010985 57010098 ID=MsaT013595.1.exon1;Parent=MsaT013595.1
Chr3 CDS 57010098 57010985 57010098 ID=cds.MsaT013595.1;Parent=MsaT013595.1
Gene Sequence

>MsaT013595.1

ATGTCTTCCTCAAATTCACCATGTGCAGCATGCAAGTTTCTCAGACGAAAGTGCACTCAAGAATGTGATTTTGCTCCTTATTTTCTCCCTGAAAATCCACAGCGATTCGCTAATGTTCACCGTGTCTTCGGTGCAAGCAACGTCGCTAAGCTATTGAAAGAGCTTGACGTGAAGGACCGTGAAGACGCGGTGAAATCTTTGGCATACGAGGCAGAGTCTCGTATAAAAGACCCTGTCTATGGATGTGTAGGTCTCATCTCATTTCTCCAACAGAAGTTAAGAGAAACTCAAAATGAACTCACTGTGGCGAAAAAAGAACTCGCCGGTTATAATTTGAATCCTCAAACCATGCAGTGCTTGTTGATGAATTCTGGAACAGTTTTAATGCCTCAGCAGCAACAGCAGCAGCAGCAATGGAATTCGCAGTTTCCGGGTTTTGTGAATTACCCTAACAATTTTCCTCAAGAGATGGTTGGAGCAGGTGGACTAGTTATGCAGCAGCAACAAGAACATGACCTTCAGATGGGTGGTGGATATCAACTTACTCCACAAGAGTATTTGCTTAGGTCTGGTACTGGTGGTGGATATCAGATGGGTGGTGGACATCAGATGGGTGCTGCTGCTGGTGGTGTTGCTGTTTCTGATCATGCTGATGTGGCTCATCCTTTGGGCATGGGAAATTTTGACAATGGTGCTACTGGTGGTGTCGCCGCCGCCGCCGGCGGGGGTTATTATCAGATTCCACAGCAAGGAGGAGAGCAACAACATAATTATCATGTTGGTGTTGCTCATGGTAATCCAGTTGAGGCACAGCTTTTGTTATCTCCACAGAATCAAGAAGCTGGTGAGGATGGTAAAAGTGTTGGTAGTGCTCATCGTTCAAGTTGA

Protein sequence

>MsaT013595.1

MSSSNSPCAACKFLRRKCTQECDFAPYFLPENPQRFANVHRVFGASNVAKLLKELDVKDREDAVKSLAYEAESRIKDPVYGCVGLISFLQQKLRETQNELTVAKKELAGYNLNPQTMQCLLMNSGTVLMPQQQQQQQQWNSQFPGFVNYPNNFPQEMVGAGGLVMQQQQEHDLQMGGGYQLTPQEYLLRSGTGGGYQMGGGHQMGAAAGGVAVSDHADVAHPLGMGNFDNGATGGVAAAAGGGYYQIPQQGGEQQHNYHVGVAHGNPVEAQLLLSPQNQEAGEDGKSVGSAHRSS*