Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013697 | XP_003600003.2 | 71.759 | 216 | 43 | 3 | 19 | 220 | 3 | 214 | 2.23e-105 | 317 |
| MsaG013697 | XP_003600003.2 | 75.385 | 65 | 16 | 0 | 244 | 308 | 171 | 235 | 1.10e-21 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013697 | sp|Q9LT81|C3H39_ARATH | 30.447 | 358 | 131 | 11 | 23 | 304 | 61 | 376 | 1.74e-39 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013697 | tr|G7J1X0|G7J1X0_MEDTR | 71.759 | 216 | 43 | 3 | 19 | 220 | 3 | 214 | 1.07e-105 | 317 |
| MsaG013697 | tr|G7J1X0|G7J1X0_MEDTR | 75.385 | 65 | 16 | 0 | 244 | 308 | 171 | 235 | 5.25e-22 | 102 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG013697 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG013697 | MsaG013765 | 0.841862 | 6.821153e-58 | 2.824287e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013697 | MtrunA17_Chr3g0098251 | 82.778 | 180 | 25 | 1 | 19 | 192 | 3 | 182 | 9.28e-108 | 311 |
| MsaG013697 | MtrunA17_Chr8g0360671 | 90.756 | 119 | 9 | 1 | 71 | 187 | 40 | 158 | 5.37e-77 | 232 |
| MsaG013697 | MtrunA17_Chr4g0063401 | 51.869 | 214 | 69 | 6 | 5 | 184 | 17 | 230 | 1.63e-62 | 198 |
| MsaG013697 | MtrunA17_Chr4g0063361 | 45.133 | 226 | 82 | 5 | 5 | 188 | 17 | 242 | 4.77e-54 | 176 |
| MsaG013697 | MtrunA17_Chr4g0063391 | 44.091 | 220 | 93 | 7 | 3 | 197 | 1 | 215 | 7.31e-48 | 160 |
| MsaG013697 | MtrunA17_Chr4g0063371 | 37.931 | 203 | 87 | 4 | 3 | 190 | 1 | 179 | 8.44e-31 | 115 |
| MsaG013697 | MtrunA17_Chr8g0366501 | 39.683 | 126 | 55 | 3 | 44 | 151 | 86 | 208 | 9.78e-21 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013697 | AT3G19360.1 | 30.447 | 358 | 131 | 11 | 23 | 304 | 61 | 376 | 1.77e-40 | 145 |
| MsaG013697 | AT3G19360.2 | 30.447 | 358 | 131 | 11 | 23 | 304 | 61 | 376 | 1.77e-40 | 145 |
| MsaG013697 | AT1G32360.1 | 36.719 | 128 | 55 | 3 | 44 | 148 | 85 | 209 | 9.18e-19 | 86.3 |
Find 83 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGAATGGATGCAGTTTC+TGG | 0.186612 | 3:+59335563 | MsaT013697.1:CDS |
| TCCTCTGCACTTGGCTAGTT+GGG | 0.297541 | 3:-59337058 | None:intergenic |
| TTCCAGTAAGTGCCCCTTGC+TGG | 0.303703 | 3:-59335786 | None:intergenic |
| GGCTAGTTGGGTAGAATTAG+AGG | 0.330401 | 3:-59337046 | None:intergenic |
| CCGCCTGTTTCTTTGTTTGA+TGG | 0.330855 | 3:-59335402 | None:intergenic |
| ATAGGCTTAGGGATGATTCT+TGG | 0.341417 | 3:+59335679 | MsaT013697.1:CDS |
| CTCGTATTTGTCATAAGTTC+AGG | 0.342100 | 3:+59335439 | MsaT013697.1:CDS |
| AGCCAGCAAGGGGCACTTAC+TGG | 0.351537 | 3:+59335784 | MsaT013697.1:CDS |
| CTCCTCTGCACTTGGCTAGT+TGG | 0.355356 | 3:-59337059 | None:intergenic |
| TATGTTTGACTGCAACATTA+TGG | 0.355765 | 3:-59336654 | None:intergenic |
| TAAGGAAGCCACCGCCGAAT+TGG | 0.369861 | 3:+59335517 | MsaT013697.1:CDS |
| CTAATAGTTTCGAAGGCAAT+AGG | 0.370078 | 3:+59335751 | MsaT013697.1:CDS |
| TTGCCACACCTAGACTTGTT+GGG | 0.387975 | 3:-59336931 | None:intergenic |
| TTTGCCACACCTAGACTTGT+TGG | 0.401025 | 3:-59336932 | None:intergenic |
| GGGCACTTACTGGAAGATTA+AGG | 0.404455 | 3:+59335794 | MsaT013697.1:CDS |
| TCTTGTTCCTTGAAAATTGA+GGG | 0.407508 | 3:-59335329 | None:intergenic |
| ACCTAGACTTGTTGGGATGC+TGG | 0.423276 | 3:-59336924 | None:intergenic |
| CTCTTGTTCCTTGAAAATTG+AGG | 0.430344 | 3:-59335330 | None:intergenic |
| AGAAGAAGCCTTTACTTTGG+TGG | 0.434420 | 3:+59336972 | MsaT013697.1:CDS |
| ATTTGTCATAAGTTCAGGTT+TGG | 0.439137 | 3:+59335444 | MsaT013697.1:CDS |
| TTTAATTTCCACCAAAGTAA+AGG | 0.443077 | 3:-59336980 | None:intergenic |
| CTTCCATCAAACAAAGAAAC+AGG | 0.444809 | 3:+59335399 | MsaT013697.1:CDS |
| TCAATACTATATATGCTGAT+TGG | 0.453146 | 3:+59337011 | MsaT013697.1:CDS |
| AATGGTGAGGAATGTCCTTA+TGG | 0.454214 | 3:+59335636 | MsaT013697.1:CDS |
| GTTTCTTTGTTTGATGGAAG+AGG | 0.456265 | 3:-59335396 | None:intergenic |
| CACTATGGTCATGGAGAAGC+AGG | 0.459706 | 3:+59335822 | MsaT013697.1:CDS |
| CTAAGAAGAAGCCTTTACTT+TGG | 0.468701 | 3:+59336969 | MsaT013697.1:CDS |
| AAGATATCGTTGTGGTGGTA+TGG | 0.470997 | 3:-59335297 | None:intergenic |
| CTGGCTCTCGCAACGAGGAA+TGG | 0.475242 | 3:+59335550 | MsaT013697.1:CDS |
| CTCACGGGGTTGGTGAGATA+AGG | 0.482058 | 3:+59335499 | MsaT013697.1:CDS |
| TCTAGGTGTGGCAAAGGAAG+TGG | 0.482685 | 3:+59336940 | MsaT013697.1:CDS |
| AGCTCCTTCCAATTCGGCGG+TGG | 0.483397 | 3:-59335525 | None:intergenic |
| TTTCTTTGTTTGATGGAAGA+GGG | 0.487321 | 3:-59335395 | None:intergenic |
| TAGGTACAATGTTCACCTTT+CGG | 0.492110 | 3:-59335474 | None:intergenic |
| AAACAACACTTATCACCATA+AGG | 0.495083 | 3:-59335651 | None:intergenic |
| CAGCAAATCATTCACAAGAT+GGG | 0.499225 | 3:+59335594 | MsaT013697.1:CDS |
| CCAGCTAGCTCCTTCCAATT+CGG | 0.500119 | 3:-59335531 | None:intergenic |
| GGCCTATGGCAGAAACATGC+TGG | 0.502157 | 3:-59336257 | None:intergenic |
| TCAGCAAATCATTCACAAGA+TGG | 0.508010 | 3:+59335593 | MsaT013697.1:CDS |
| AACATTGTACCTATGCTCAC+GGG | 0.508915 | 3:+59335484 | MsaT013697.1:CDS |
| ATCCAGCATGTTTCTGCCAT+AGG | 0.513911 | 3:+59336255 | MsaT013697.1:CDS |
| CAAGCATGGACTGTGGCCTA+TGG | 0.516821 | 3:-59336271 | None:intergenic |
| CCGAATTGGAAGGAGCTAGC+TGG | 0.521647 | 3:+59335531 | MsaT013697.1:CDS |
| CCACTGTAGCCTATGGTGGC+TGG | 0.522317 | 3:-59336225 | None:intergenic |
| AAGATTAAGGACTGTCACTA+TGG | 0.525056 | 3:+59335807 | MsaT013697.1:CDS |
| AAAATAGCCACTGTAGCCTA+TGG | 0.544445 | 3:-59336232 | None:intergenic |
| GTCTGTGAACAAGCCAGCAA+GGG | 0.545575 | 3:+59335773 | MsaT013697.1:CDS |
| ACTTCCAATGCTTAATGCAC+AGG | 0.545801 | 3:-59335715 | None:intergenic |
| TGTACCTATGCTCACGGGGT+TGG | 0.546278 | 3:+59335489 | MsaT013697.1:CDS |
| GATGGTTCTAATAGTTTCGA+AGG | 0.547401 | 3:+59335744 | MsaT013697.1:CDS |
| GATGTTGTGCCAGCCACCAT+AGG | 0.549823 | 3:+59336216 | MsaT013697.1:CDS |
| GGTCTGTGAACAAGCCAGCA+AGG | 0.553295 | 3:+59335772 | MsaT013697.1:CDS |
| GAGGCCTGTGCATTAAGCAT+TGG | 0.559524 | 3:+59335711 | MsaT013697.1:CDS |
| AAGAGGGCATATGAATGCAT+TGG | 0.560421 | 3:-59335379 | None:intergenic |
| GCAGTGTTATCATGAGTGAA+AGG | 0.561491 | 3:-59336327 | None:intergenic |
| CATGAGTGAAAGGAAGGCTA+TGG | 0.562910 | 3:-59336317 | None:intergenic |
| AGGTTTGGTAATTGTCCGAA+AGG | 0.564006 | 3:+59335459 | MsaT013697.1:CDS |
| GCATTAAGCATTGGAAGTAT+CGG | 0.572339 | 3:+59335720 | MsaT013697.1:CDS |
| ACCAGCATCCCAACAAGTCT+AGG | 0.572783 | 3:+59336923 | MsaT013697.1:CDS |
| AAGGACTGTCACTATGGTCA+TGG | 0.572929 | 3:+59335813 | MsaT013697.1:CDS |
| AACAAGTCTAGGTGTGGCAA+AGG | 0.574700 | 3:+59336934 | MsaT013697.1:CDS |
| GAACATTGTACCTATGCTCA+CGG | 0.579167 | 3:+59335483 | MsaT013697.1:CDS |
| GAAGCCACCGCCGAATTGGA+AGG | 0.582073 | 3:+59335521 | MsaT013697.1:CDS |
| TGTTATCATGAGTGAAAGGA+AGG | 0.585694 | 3:-59336323 | None:intergenic |
| AGCATTGGAAGTATCGGAGA+TGG | 0.590771 | 3:+59335726 | MsaT013697.1:CDS |
| AGCAAATCATTCACAAGATG+GGG | 0.600236 | 3:+59335595 | MsaT013697.1:CDS |
| TTTCAAAGATATCGTTGTGG+TGG | 0.607400 | 3:-59335302 | None:intergenic |
| CTCACCAACCCCGTGAGCAT+AGG | 0.617079 | 3:-59335493 | None:intergenic |
| GCTAGCTCCTTCCAATTCGG+CGG | 0.629242 | 3:-59335528 | None:intergenic |
| ATAGCCACTGTAGCCTATGG+TGG | 0.635922 | 3:-59336229 | None:intergenic |
| CATCCCAACAAGTCTAGGTG+TGG | 0.656724 | 3:+59336928 | MsaT013697.1:CDS |
| CAAGAAATACTACAATGGTG+AGG | 0.666991 | 3:+59335623 | MsaT013697.1:CDS |
| TCTGTGAACAAGCCAGCAAG+GGG | 0.667598 | 3:+59335774 | MsaT013697.1:CDS |
| CTATGCAAGAAATACTACAA+TGG | 0.669884 | 3:+59335618 | MsaT013697.1:CDS |
| TGATAACACTGCTCTATCCG+AGG | 0.674059 | 3:+59336338 | MsaT013697.1:CDS |
| TGATTCTTGGAAATCAAGAG+AGG | 0.676002 | 3:+59335692 | MsaT013697.1:CDS |
| CCAGCCACCATAGGCTACAG+TGG | 0.677219 | 3:+59336225 | MsaT013697.1:CDS |
| CCATCAAACAAAGAAACAGG+CGG | 0.683642 | 3:+59335402 | MsaT013697.1:CDS |
| TCGTTTCAAAGATATCGTTG+TGG | 0.683730 | 3:-59335305 | None:intergenic |
| ACATTGTACCTATGCTCACG+GGG | 0.692970 | 3:+59335485 | MsaT013697.1:CDS |
| TGGCATACAAGCATGGACTG+TGG | 0.706120 | 3:-59336278 | None:intergenic |
| GCTAGCTGGCTCTCGCAACG+AGG | 0.719790 | 3:+59335545 | MsaT013697.1:CDS |
| ATAACGGCACCAAGGCATGG+AGG | 0.742684 | 3:+59336194 | MsaT013697.1:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 59335282 | 59337066 | 59335282 | ID=MsaG013697 |
| Chr3 | mRNA | 59335282 | 59337066 | 59335282 | ID=MsaT013697.1;Parent=MsaG013697 |
| Chr3 | exon | 59335282 | 59335843 | 59335282 | ID=MsaT013697.1.exon1;Parent=MsaT013697.1 |
| Chr3 | CDS | 59335282 | 59335843 | 59335282 | ID=cds.MsaT013697.1;Parent=MsaT013697.1 |
| Chr3 | exon | 59336208 | 59336359 | 59336208 | ID=MsaT013697.1.exon2;Parent=MsaT013697.1 |
| Chr3 | CDS | 59336208 | 59336359 | 59336208 | ID=cds.MsaT013697.1;Parent=MsaT013697.1 |
| Chr3 | exon | 59336628 | 59336676 | 59336628 | ID=MsaT013697.1.exon3;Parent=MsaT013697.1 |
| Chr3 | CDS | 59336628 | 59336676 | 59336628 | ID=cds.MsaT013697.1;Parent=MsaT013697.1 |
| Chr3 | exon | 59336903 | 59337066 | 59336903 | ID=MsaT013697.1.exon4;Parent=MsaT013697.1 |
| Chr3 | CDS | 59336903 | 59337066 | 59336903 | ID=cds.MsaT013697.1;Parent=MsaT013697.1 |
| Gene Sequence |
| Protein sequence |
