Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013755 | AES70281.2 | 85.079 | 315 | 43 | 1 | 1 | 315 | 1 | 311 | 9.13e-179 | 509 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013755 | sp|Q7XTT4|NUCL2_ORYSJ | 52.055 | 73 | 34 | 1 | 254 | 325 | 431 | 503 | 1.34e-18 | 90.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013755 | tr|G7J1Z7|G7J1Z7_MEDTR | 85.079 | 315 | 43 | 1 | 1 | 315 | 1 | 311 | 4.36e-179 | 509 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG013755 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG001907 | MsaG013755 | 0.847882 | 1.650259e-59 | 8.324764e-57 |
| MsaG004369 | MsaG013755 | 0.812524 | 6.980330e-51 | 1.268196e-48 |
| MsaG011425 | MsaG013755 | 0.810963 | 1.520301e-50 | 2.657936e-48 |
| MsaG046696 | MsaG013755 | 0.808298 | 5.646792e-50 | 9.255547e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013755 | MtrunA17_Chr3g0098461 | 84.906 | 159 | 24 | 0 | 1 | 159 | 1 | 159 | 6.90e-97 | 283 |
| MsaG013755 | MtrunA17_Chr3g0098471 | 84.884 | 172 | 22 | 1 | 158 | 329 | 20 | 187 | 1.10e-84 | 253 |
| MsaG013755 | MtrunA17_Chr0c05g0492161 | 67.290 | 107 | 35 | 0 | 1 | 107 | 1 | 107 | 1.29e-45 | 151 |
| MsaG013755 | MtrunA17_Chr4g0038111 | 71.233 | 73 | 21 | 0 | 253 | 325 | 321 | 393 | 4.35e-28 | 114 |
| MsaG013755 | MtrunA17_Chr1g0163591 | 51.948 | 77 | 36 | 1 | 250 | 325 | 271 | 347 | 4.48e-19 | 88.2 |
| MsaG013755 | MtrunA17_Chr1g0163591 | 35.859 | 198 | 95 | 7 | 159 | 325 | 20 | 216 | 5.39e-19 | 87.8 |
| MsaG013755 | MtrunA17_Chr3g0090311 | 33.544 | 158 | 93 | 6 | 6 | 153 | 2 | 157 | 2.63e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG013755 | AT3G18610.3 | 47.436 | 78 | 40 | 1 | 248 | 325 | 332 | 408 | 1.49e-17 | 84.0 |
| MsaG013755 | AT3G18610.1 | 47.436 | 78 | 40 | 1 | 248 | 325 | 362 | 438 | 1.52e-17 | 84.0 |
| MsaG013755 | AT3G18610.2 | 47.436 | 78 | 40 | 1 | 248 | 325 | 302 | 378 | 1.65e-17 | 83.6 |
| MsaG013755 | AT1G48920.1 | 42.697 | 89 | 49 | 2 | 238 | 325 | 265 | 352 | 3.43e-16 | 79.7 |
| MsaG013755 | AT5G13780.1 | 38.136 | 118 | 67 | 5 | 37 | 151 | 41 | 155 | 6.40e-11 | 61.2 |
Find 75 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAGGATGGAAAGCTTAA+AGG | 0.173752 | 3:+60397375 | MsaT013755.1:CDS |
| TGCGGGTGCACTTCCTATTT+TGG | 0.183725 | 3:-60396826 | None:intergenic |
| TAAAGATCGTCTTTCTTTAC+AGG | 0.196172 | 3:-60396611 | None:intergenic |
| GAAGTTGTTCCTTCCAAAAT+AGG | 0.222757 | 3:+60396813 | MsaT013755.1:CDS |
| CAGAAGATTCGTCAGATTCT+TGG | 0.263120 | 3:-60396869 | None:intergenic |
| GAACTATCAGCCTTCTTTGC+GGG | 0.314916 | 3:-60396843 | None:intergenic |
| TCATCTTCTGAAGAATCTTC+TGG | 0.335928 | 3:+60396638 | MsaT013755.1:CDS |
| TGTTGACCTATATAAGATTC+TGG | 0.336459 | 3:+60390150 | MsaT013755.1:CDS |
| TTCTACAACGATGGTGTCCC+CGG | 0.363342 | 3:+60390102 | None:intergenic |
| ACTTGTGACCATGGCTGAAA+TGG | 0.368546 | 3:+60390369 | MsaT013755.1:CDS |
| CAAGAATCTGACGAATCTTC+TGG | 0.369595 | 3:+60396870 | MsaT013755.1:CDS |
| ATGTCAAGAGCTTACGTATT+GGG | 0.379625 | 3:-60397433 | None:intergenic |
| ATCCATCAGAGTTGGTCTTC+GGG | 0.381778 | 3:-60394999 | None:intergenic |
| TCTTCGTTAGCCCTATCTTT+CGG | 0.385024 | 3:+60390326 | MsaT013755.1:CDS |
| GATGGAAAGCTTAAAGGCTT+TGG | 0.397596 | 3:+60397381 | MsaT013755.1:CDS |
| ATTACTCTTCTTCGAAAAGA+TGG | 0.410597 | 3:+60395020 | MsaT013755.1:CDS |
| TGACGTGGATACTCAAAGTT+TGG | 0.414598 | 3:+60390189 | MsaT013755.1:CDS |
| AATCCATCAGAGTTGGTCTT+CGG | 0.430864 | 3:-60395000 | None:intergenic |
| AGCCCTATCTTTCGGAGAAA+AGG | 0.431404 | 3:+60390334 | MsaT013755.1:CDS |
| TGTACGTCTTGCTTCTCATA+AGG | 0.432229 | 3:+60397359 | MsaT013755.1:CDS |
| GTGAAGAGCAATTATATTGA+TGG | 0.434863 | 3:+60395060 | MsaT013755.1:CDS |
| AACGATGGTGTCCCCGGGTG+TGG | 0.437378 | 3:+60390108 | None:intergenic |
| AGAATCTTATATAGGTCAAC+AGG | 0.439517 | 3:-60390148 | None:intergenic |
| GGCTGAAATGGACTTGGAAA+AGG | 0.444198 | 3:+60390381 | MsaT013755.1:CDS |
| GAAGGAACAACTTCTGCTGC+AGG | 0.453852 | 3:-60396804 | None:intergenic |
| TATGTTGGGAACTTATCCTT+CGG | 0.474371 | 3:+60397206 | MsaT013755.1:CDS |
| TCAGAGTTGGTCTTCGGGAC+GGG | 0.477721 | 3:-60394994 | None:intergenic |
| TGAGGTTGCTGCCAAACAAA+AGG | 0.484062 | 3:+60396390 | MsaT013755.1:CDS |
| TTACTCTTCTTCGAAAAGAT+GGG | 0.485859 | 3:+60395021 | MsaT013755.1:CDS |
| GAACTGAAGCGTACTAGTTA+CGG | 0.493616 | 3:+60390289 | MsaT013755.1:CDS |
| AGGAAGTGCACCCGCAAAGA+AGG | 0.501050 | 3:+60396833 | MsaT013755.1:CDS |
| AGTTTCTTCAAAGAAGCAAA+AGG | 0.509361 | 3:+60396363 | MsaT013755.1:CDS |
| TTCCAAGTCCATTTCAGCCA+TGG | 0.509729 | 3:-60390377 | None:intergenic |
| GACCATGGCTGAAATGGACT+TGG | 0.509783 | 3:+60390375 | MsaT013755.1:CDS |
| GGTTACATCGTCTTTGATGT+TGG | 0.509911 | 3:+60390265 | MsaT013755.1:CDS |
| CATTCACCAGAATCTTATAT+AGG | 0.510974 | 3:-60390156 | None:intergenic |
| GATCTCCTAGGCATTATCTT+CGG | 0.511416 | 3:+60390244 | MsaT013755.1:CDS |
| GTGTGAAGACATTATATGTT+GGG | 0.513802 | 3:+60397192 | MsaT013755.1:CDS |
| CGTGTGAAGACATTATATGT+TGG | 0.515977 | 3:+60397191 | MsaT013755.1:CDS |
| GCGTCTCTTACGACCACACC+CGG | 0.518110 | 3:-60390121 | None:intergenic |
| GATGTCAAGAGCTTACGTAT+TGG | 0.518828 | 3:-60397434 | None:intergenic |
| AGAACTATCAGCCTTCTTTG+CGG | 0.521392 | 3:-60396844 | None:intergenic |
| CGTCTTGCTTCTCATAAGGA+TGG | 0.533795 | 3:+60397363 | MsaT013755.1:CDS |
| GATACTCAAAGTTTGGTGAA+TGG | 0.534335 | 3:+60390196 | MsaT013755.1:CDS |
| GTCCCGAAGACCAACTCTGA+TGG | 0.538106 | 3:+60394997 | MsaT013755.1:CDS |
| CGCTACTCGACTTGTGACCA+TGG | 0.544412 | 3:+60390360 | MsaT013755.1:CDS |
| CGAATCTTCTGGTGAAGATG+AGG | 0.546781 | 3:+60396881 | MsaT013755.1:CDS |
| TGAAGAGCAATTATATTGAT+GGG | 0.547887 | 3:+60395061 | MsaT013755.1:CDS |
| GAGCAATTATATTGATGGGA+AGG | 0.549182 | 3:+60395065 | MsaT013755.1:CDS |
| ATCAGAGTTGGTCTTCGGGA+CGG | 0.551912 | 3:-60394995 | None:intergenic |
| AAACTTTGAGTATCCACGTC+AGG | 0.555864 | 3:-60390187 | None:intergenic |
| TCTACAACGATGGTGTCCCC+GGG | 0.568555 | 3:+60390103 | None:intergenic |
| GCAAGCTGTGGAAGAAGTCG+AGG | 0.568949 | 3:+60396339 | MsaT013755.1:CDS |
| ATCATGACGAAGAAGTTCGA+GGG | 0.572521 | 3:+60395102 | MsaT013755.1:CDS |
| CGAAAAGATGGGGTATGAGA+AGG | 0.577206 | 3:+60395032 | MsaT013755.1:CDS |
| GAAGAGTAATCCATCAGAGT+TGG | 0.583113 | 3:-60395007 | None:intergenic |
| TACTCTTCTTCGAAAAGATG+GGG | 0.590062 | 3:+60395022 | MsaT013755.1:CDS |
| CATCATGACGAAGAAGTTCG+AGG | 0.591689 | 3:+60395101 | MsaT013755.1:CDS |
| GTTGCAGCGGATGATCTCCT+AGG | 0.593344 | 3:+60390232 | MsaT013755.1:CDS |
| GAAAGATAGGGCTAACGAAG+AGG | 0.596707 | 3:-60390324 | None:intergenic |
| TGTTGAGTTTGCAACTGCAG+AGG | 0.598820 | 3:+60397407 | MsaT013755.1:CDS |
| AAAGAAGCAAAAGGTAGATG+AGG | 0.604240 | 3:+60396372 | MsaT013755.1:CDS |
| AAAGATAGGGCTAACGAAGA+GGG | 0.608748 | 3:-60390323 | None:intergenic |
| ATGCATTATGCGGAGACCGA+AGG | 0.609027 | 3:+60395132 | MsaT013755.1:CDS |
| TTGACTTTGATATAGGCAAG+AGG | 0.619505 | 3:+60396317 | MsaT013755.1:intron |
| CGTCTCTTACGACCACACCC+GGG | 0.631420 | 3:-60390120 | None:intergenic |
| AAAGATGGGGTATGAGAAGG+AGG | 0.631960 | 3:+60395035 | MsaT013755.1:CDS |
| TGTAACCGAAGATAATGCCT+AGG | 0.649259 | 3:-60390249 | None:intergenic |
| TATAGGCAAGAGGCAAGCTG+TGG | 0.650683 | 3:+60396327 | MsaT013755.1:intron |
| GTCTCTTACGACCACACCCG+GGG | 0.653034 | 3:-60390119 | None:intergenic |
| TGAATCTTCTGATGAAGATG+AGG | 0.655782 | 3:+60396661 | MsaT013755.1:CDS |
| GGGGAAGCAGATGCATTATG+CGG | 0.657065 | 3:+60395122 | MsaT013755.1:CDS |
| TAAGCTTCTTCAAGTTGCAG+CGG | 0.657667 | 3:+60390219 | MsaT013755.1:CDS |
| GAATGATGTCTTTCCTGACG+TGG | 0.685326 | 3:+60390174 | MsaT013755.1:CDS |
| TCATGACGAAGAAGTTCGAG+GGG | 0.691102 | 3:+60395103 | MsaT013755.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 60390112 | 60397452 | 60390112 | ID=MsaG013755 |
| Chr3 | mRNA | 60390112 | 60397452 | 60390112 | ID=MsaT013755.1;Parent=MsaG013755 |
| Chr3 | exon | 60390112 | 60390402 | 60390112 | ID=MsaT013755.1.exon1;Parent=MsaT013755.1 |
| Chr3 | CDS | 60390112 | 60390402 | 60390112 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60394970 | 60395153 | 60394970 | ID=MsaT013755.1.exon2;Parent=MsaT013755.1 |
| Chr3 | CDS | 60394970 | 60395153 | 60394970 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60396332 | 60396411 | 60396332 | ID=MsaT013755.1.exon3;Parent=MsaT013755.1 |
| Chr3 | CDS | 60396332 | 60396411 | 60396332 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60396569 | 60396682 | 60396569 | ID=MsaT013755.1.exon4;Parent=MsaT013755.1 |
| Chr3 | CDS | 60396569 | 60396682 | 60396569 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60396801 | 60396902 | 60396801 | ID=MsaT013755.1.exon5;Parent=MsaT013755.1 |
| Chr3 | CDS | 60396801 | 60396902 | 60396801 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60397158 | 60397246 | 60397158 | ID=MsaT013755.1.exon6;Parent=MsaT013755.1 |
| Chr3 | CDS | 60397158 | 60397246 | 60397158 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Chr3 | exon | 60397323 | 60397452 | 60397323 | ID=MsaT013755.1.exon7;Parent=MsaT013755.1 |
| Chr3 | CDS | 60397323 | 60397452 | 60397323 | ID=cds.MsaT013755.1;Parent=MsaT013755.1 |
| Gene Sequence |
| Protein sequence |
