AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013879

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013879 MtrunA17_Chr3g0099641 90.985 477 34 3 1 477 1 468 0.0 858
MsaG013879 MtrunA17_Chr2g0288871 42.827 481 206 12 33 454 60 530 7.55e-96 300
MsaG013879 MtrunA17_Chr2g0322391 40.486 494 204 16 35 455 81 557 1.02e-88 283
MsaG013879 MtrunA17_Chr4g0075201 39.035 456 204 13 35 442 36 465 6.93e-85 270
MsaG013879 MtrunA17_Chr7g0270831 36.726 452 204 12 23 454 13 402 3.58e-70 229
MsaG013879 MtrunA17_Chr4g0025051 35.590 458 206 14 88 461 100 552 2.27e-63 216
MsaG013879 MtrunA17_Chr4g0025061 35.099 453 195 15 94 462 131 568 5.57e-63 215
MsaG013879 MtrunA17_Chr2g0302391 47.210 233 110 6 234 457 185 413 1.47e-57 196
MsaG013879 MtrunA17_Chr4g0024391 33.559 444 209 11 96 456 2 442 1.29e-56 195
MsaG013879 MtrunA17_Chr7g0216891 30.444 450 212 12 101 458 47 487 5.89e-46 168
MsaG013879 MtrunA17_Chr4g0051181 39.695 262 118 7 244 468 227 485 8.35e-42 155
MsaG013879 MtrunA17_Chr4g0060271 69.767 86 26 0 235 320 230 315 1.39e-38 148
MsaG013879 MtrunA17_Chr5g0419241 58.889 90 33 1 199 288 20 105 2.37e-29 111
MsaG013879 MtrunA17_Chr4g0001361 33.178 214 101 6 243 456 145 316 1.83e-28 114
MsaG013879 MtrunA17_Chr1g0150271 47.368 95 48 1 244 336 83 177 1.73e-25 105
MsaG013879 MtrunA17_Chr3g0136961 28.958 259 121 6 60 318 1 196 3.92e-25 105
MsaG013879 MtrunA17_Chr5g0432161 47.191 89 46 1 232 320 393 480 1.04e-23 104
MsaG013879 MtrunA17_Chr5g0432161 50.000 60 28 2 261 320 245 302 8.24e-12 67.4
MsaG013879 MtrunA17_Chr5g0445311 52.564 78 36 1 243 320 347 423 1.77e-23 103
MsaG013879 MtrunA17_Chr4g0004911 38.971 136 72 4 193 320 263 395 1.91e-23 103
MsaG013879 MtrunA17_Chr4g0004911 35.955 89 56 1 241 329 165 252 5.01e-13 71.2
MsaG013879 MtrunA17_Chr3g0101131 52.564 78 36 1 243 320 398 474 2.12e-23 103
MsaG013879 MtrunA17_Chr5g0447701 45.361 97 51 2 234 330 226 320 2.41e-23 101
MsaG013879 MtrunA17_Chr1g0150281 32.536 209 83 6 245 453 125 275 4.67e-23 99.0
MsaG013879 MtrunA17_Chr7g0218081 45.918 98 49 2 239 336 389 482 5.33e-23 102
MsaG013879 MtrunA17_Chr7g0218081 53.333 60 26 2 261 320 229 286 4.27e-13 71.2
MsaG013879 MtrunA17_Chr3g0106491 44.944 89 48 1 232 320 337 424 7.99e-23 101
MsaG013879 MtrunA17_Chr3g0106491 48.333 60 29 2 261 320 199 256 1.03e-11 67.0
MsaG013879 MtrunA17_Chr3g0079691 40.496 121 69 2 243 361 478 597 1.12e-22 101
MsaG013879 MtrunA17_Chr3g0079691 42.683 82 44 3 261 341 281 360 9.45e-14 73.9
MsaG013879 MtrunA17_Chr7g0243891 40.602 133 67 5 197 320 304 433 1.83e-22 100
MsaG013879 MtrunA17_Chr7g0243891 41.333 75 42 2 261 335 217 289 8.37e-11 64.3
MsaG013879 MtrunA17_Chr4g0051881 43.956 91 50 1 247 337 165 254 2.61e-22 97.8
MsaG013879 MtrunA17_Chr2g0333851 29.680 219 98 6 237 450 142 309 3.32e-22 97.1
MsaG013879 MtrunA17_Chr5g0418191 46.988 83 43 1 238 320 298 379 4.38e-22 98.6
MsaG013879 MtrunA17_Chr5g0418191 47.222 72 36 2 261 332 152 221 3.30e-13 71.2
MsaG013879 MtrunA17_Chr2g0294231 48.148 81 41 1 244 324 187 266 5.13e-22 97.4
MsaG013879 MtrunA17_Chr4g0042831 47.561 82 42 1 239 320 257 337 1.39e-21 96.3
MsaG013879 MtrunA17_Chr4g0042831 42.500 80 43 2 261 340 146 222 3.73e-11 64.7
MsaG013879 MtrunA17_Chr5g0443131 49.383 81 40 1 247 327 144 223 2.84e-21 94.4
MsaG013879 MtrunA17_Chr7g0270191 42.857 98 51 2 244 341 129 221 3.63e-21 93.6
MsaG013879 MtrunA17_Chr7g0249311 48.718 78 39 1 243 320 375 451 5.42e-21 96.3
MsaG013879 MtrunA17_Chr7g0249311 46.875 64 32 2 261 324 180 241 6.74e-12 67.8
MsaG013879 MtrunA17_Chr7g0249301 48.718 78 39 1 243 320 275 351 1.19e-20 93.6
MsaG013879 MtrunA17_Chr7g0249301 44.928 69 36 2 256 324 58 124 2.23e-12 68.6
MsaG013879 MtrunA17_Chr4g0068541 46.341 82 43 1 244 325 192 272 1.63e-20 93.6
MsaG013879 MtrunA17_Chr5g0418781 46.914 81 42 1 237 317 101 180 2.44e-20 90.9
MsaG013879 MtrunA17_Chr1g0194481 42.857 98 54 2 244 340 142 238 2.45e-20 91.7
MsaG013879 MtrunA17_Chr7g0224801 50.667 75 36 1 249 323 82 155 2.64e-20 88.2
MsaG013879 MtrunA17_Chr1g0150801 49.315 73 36 1 245 317 87 158 6.64e-20 86.7
MsaG013879 MtrunA17_Chr7g0263531 46.988 83 43 1 238 320 339 420 8.29e-20 91.7
MsaG013879 MtrunA17_Chr7g0263531 44.000 75 40 2 261 335 190 262 8.35e-13 70.1
MsaG013879 MtrunA17_Chr8g0386241 42.857 84 47 1 243 326 86 168 1.68e-19 86.3
MsaG013879 MtrunA17_Chr3g0129321 50.000 74 36 1 247 320 105 177 4.59e-19 85.5
MsaG013879 MtrunA17_Chr1g0184691 51.389 72 35 0 256 327 289 360 4.78e-19 88.6
MsaG013879 MtrunA17_Chr8g0379461 46.739 92 42 2 251 341 66 151 6.24e-19 86.3
MsaG013879 MtrunA17_Chr2g0284321 58.065 62 26 0 260 321 35 96 6.44e-19 85.5
MsaG013879 MtrunA17_Chr7g0270051 42.857 98 47 2 242 333 29 123 7.05e-19 86.3
MsaG013879 MtrunA17_Chr7g0248721 49.425 87 39 3 244 330 125 206 1.03e-18 84.7
MsaG013879 MtrunA17_Chr2g0293481 40.000 110 65 1 227 335 233 342 1.04e-18 88.6
MsaG013879 MtrunA17_Chr4g0053281 50.000 74 37 0 246 319 252 325 1.24e-18 87.0
MsaG013879 MtrunA17_Chr8g0383251 44.156 77 42 1 247 323 95 170 1.28e-18 83.6
MsaG013879 MtrunA17_Chr8g0335811 46.053 76 40 1 243 318 137 211 1.64e-18 84.3
MsaG013879 MtrunA17_Chr5g0401821 50.000 74 37 0 246 319 185 258 1.67e-18 85.5
MsaG013879 MtrunA17_Chr2g0289181 41.975 81 47 0 255 335 9 89 1.72e-18 84.7
MsaG013879 MtrunA17_Chr2g0319961 51.429 70 34 0 251 320 71 140 2.15e-18 85.1
MsaG013879 MtrunA17_Chr4g0074001 48.052 77 39 1 244 320 343 418 3.45e-18 87.0
MsaG013879 MtrunA17_Chr3g0129361 44.737 76 41 1 243 318 127 201 4.74e-18 82.8
MsaG013879 MtrunA17_Chr7g0228941 47.887 71 37 0 247 317 39 109 5.18e-18 80.1
MsaG013879 MtrunA17_Chr1g0147881 43.421 76 43 0 244 319 246 321 5.60e-18 85.1
MsaG013879 MtrunA17_Chr4g0071521 47.500 80 41 1 240 318 224 303 9.14e-18 84.0
MsaG013879 MtrunA17_Chr1g0188941 48.485 66 33 1 255 320 46 110 1.62e-17 78.2
MsaG013879 MtrunA17_Chr8g0383351 44.156 77 41 2 243 318 142 217 1.76e-16 79.0
MsaG013879 MtrunA17_Chr3g0139021 44.595 74 41 0 244 317 298 371 3.27e-16 80.5
MsaG013879 MtrunA17_Chr8g0348311 45.946 74 40 0 257 330 152 225 3.40e-16 79.7
MsaG013879 MtrunA17_Chr1g0202461 40.426 94 54 1 257 350 258 349 5.66e-16 79.7
MsaG013879 MtrunA17_Chr6g0485431 47.561 82 40 3 241 320 134 214 6.03e-16 79.3
MsaG013879 MtrunA17_Chr7g0248691 39.362 94 50 1 257 350 151 237 2.30e-15 77.0
MsaG013879 MtrunA17_Chr8g0387501 43.056 72 41 0 259 330 128 199 5.29e-15 75.1
MsaG013879 MtrunA17_Chr7g0238921 54.098 61 27 1 261 321 125 184 1.17e-14 74.7
MsaG013879 MtrunA17_Chr1g0189391 42.466 73 40 2 247 318 84 155 1.82e-13 71.6
MsaG013879 MtrunA17_Chr8g0347251 46.575 73 32 2 259 324 135 207 7.28e-12 66.6
MsaG013879 MtrunA17_Chr7g0244981 43.210 81 44 1 261 339 123 203 9.81e-12 65.5
MsaG013879 MtrunA17_Chr5g0432071 40.260 77 44 2 261 337 105 179 1.58e-11 63.9
MsaG013879 MtrunA17_Chr7g0244971 36.364 99 60 2 244 339 106 204 2.40e-11 64.7
MsaG013879 MtrunA17_Chr8g0387981 35.593 118 58 5 222 321 83 200 3.11e-11 64.7
MsaG013879 MtrunA17_Chr5g0447711 34.783 92 57 3 250 340 220 309 3.85e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013879 AT4G04450.1 40.202 495 206 18 34 453 24 503 2.07e-91 288
MsaG013879 AT4G04450.2 43.341 413 172 11 88 453 12 409 1.64e-90 283
MsaG013879 AT1G62300.1 40.426 470 221 12 35 456 84 542 6.81e-88 280
MsaG013879 AT4G22070.4 41.626 406 183 11 27 382 19 420 1.30e-74 244
MsaG013879 AT4G22070.3 41.626 406 183 11 27 382 19 420 1.30e-74 244
MsaG013879 AT4G22070.2 41.626 406 183 11 27 382 19 420 1.30e-74 244
MsaG013879 AT4G22070.1 41.626 406 183 11 27 382 19 420 1.30e-74 244
MsaG013879 AT4G01720.1 49.200 250 104 6 222 450 196 443 1.28e-53 187
MsaG013879 AT1G68150.1 40.467 257 123 9 101 356 100 327 3.79e-45 162
MsaG013879 AT5G15130.1 31.197 468 227 15 94 474 35 494 1.59e-44 164
MsaG013879 AT5G15130.2 36.441 354 161 11 182 474 102 452 1.83e-44 163
MsaG013879 AT1G18860.1 50.000 192 72 6 240 422 219 395 6.58e-41 154
MsaG013879 AT1G69810.1 33.227 313 183 8 87 382 18 321 7.73e-36 137
MsaG013879 AT1G69810.2 47.134 157 77 3 227 382 112 263 5.76e-34 130
MsaG013879 AT1G80840.1 25.377 398 186 11 57 450 1 291 2.76e-27 111
MsaG013879 AT4G31800.1 31.381 239 127 5 101 338 52 254 3.24e-27 111
MsaG013879 AT4G31800.2 38.571 140 79 1 199 338 57 189 1.59e-24 102
MsaG013879 AT1G29860.1 38.971 136 72 4 244 369 120 254 6.52e-24 101
MsaG013879 AT1G13960.2 53.012 83 38 1 238 320 234 315 1.16e-23 102
MsaG013879 AT1G13960.2 53.333 60 26 2 261 320 77 134 7.98e-13 70.1
MsaG013879 AT1G13960.1 53.012 83 38 1 238 320 387 468 1.42e-23 103
MsaG013879 AT1G13960.1 53.333 60 26 2 261 320 230 287 5.27e-13 71.6
MsaG013879 AT2G38470.1 37.879 132 67 4 243 374 345 461 1.87e-23 103
MsaG013879 AT2G38470.1 45.205 73 37 3 248 320 173 242 1.04e-11 67.4
MsaG013879 AT5G07100.2 45.361 97 47 2 240 331 121 216 3.84e-23 97.8
MsaG013879 AT5G07100.4 45.361 97 47 2 240 331 121 216 3.84e-23 97.8
MsaG013879 AT5G07100.5 45.361 97 47 2 240 331 121 216 3.84e-23 97.8
MsaG013879 AT5G07100.3 45.361 97 47 2 240 331 121 216 3.84e-23 97.8
MsaG013879 AT4G26640.5 51.282 78 37 1 243 320 188 264 4.45e-23 101
MsaG013879 AT4G26640.6 51.282 78 37 1 243 320 188 264 4.45e-23 101
MsaG013879 AT4G26640.4 51.282 78 37 1 243 320 188 264 4.45e-23 101
MsaG013879 AT4G26640.3 51.282 78 37 1 243 320 188 264 4.45e-23 101
MsaG013879 AT5G07100.1 45.361 97 47 2 240 331 214 309 6.80e-23 99.4
MsaG013879 AT4G26640.7 51.282 78 37 1 243 320 324 400 8.60e-23 101
MsaG013879 AT4G26640.1 51.282 78 37 1 243 320 292 368 9.61e-23 101
MsaG013879 AT4G26640.2 51.282 78 37 1 243 320 364 440 1.15e-22 101
MsaG013879 AT5G46350.1 42.188 128 62 4 233 350 147 272 1.20e-22 99.0
MsaG013879 AT2G03340.1 50.602 83 40 1 238 320 393 474 2.15e-22 100
MsaG013879 AT2G03340.1 56.667 60 24 2 261 320 251 308 2.45e-14 75.5
MsaG013879 AT2G25000.2 61.290 62 24 0 259 320 102 163 2.33e-22 95.9
MsaG013879 AT2G25000.4 61.290 62 24 0 259 320 102 163 2.33e-22 95.9
MsaG013879 AT2G25000.3 61.290 62 24 0 259 320 124 185 3.48e-22 95.9
MsaG013879 AT2G25000.1 61.290 62 24 0 259 320 145 206 4.09e-22 96.3
MsaG013879 AT5G26170.1 50.685 73 35 1 246 318 99 170 4.77e-22 93.6
MsaG013879 AT2G47260.1 42.609 115 58 3 244 351 158 271 4.82e-22 97.4
MsaG013879 AT5G41570.1 52.941 85 36 2 244 328 82 162 5.88e-22 93.2
MsaG013879 AT5G56270.1 49.398 83 41 1 238 320 465 546 6.57e-22 99.8
MsaG013879 AT5G56270.1 49.180 61 29 2 261 321 274 332 9.60e-12 67.8
MsaG013879 AT4G18170.1 51.948 77 36 1 244 320 156 231 7.63e-22 96.7
MsaG013879 AT3G01970.1 42.991 107 47 2 226 319 18 123 1.09e-21 91.7
MsaG013879 AT2G04880.2 48.684 76 38 1 246 321 269 343 1.16e-21 97.8
MsaG013879 AT2G04880.2 42.500 80 37 3 248 320 92 169 1.58e-11 66.6
MsaG013879 AT2G04880.1 48.684 76 38 1 246 321 293 367 1.35e-21 97.8
MsaG013879 AT2G04880.1 42.500 80 37 3 248 320 92 169 1.88e-11 66.6
MsaG013879 AT5G49520.1 44.944 89 48 1 244 332 205 292 2.02e-21 96.7
MsaG013879 AT1G64000.1 53.659 82 34 2 247 328 101 178 2.59e-21 92.0
MsaG013879 AT3G01080.1 48.718 78 39 1 243 320 289 365 4.26e-21 95.9
MsaG013879 AT3G01080.1 50.000 60 28 2 261 320 168 225 2.47e-11 65.9
MsaG013879 AT3G01080.2 48.718 78 39 1 243 320 327 403 4.73e-21 95.9
MsaG013879 AT3G01080.2 50.000 60 28 2 261 320 206 263 2.56e-11 66.2
MsaG013879 AT2G30250.1 51.282 78 37 1 243 320 311 387 5.00e-21 95.1
MsaG013879 AT2G46130.1 44.898 98 50 2 233 330 3 96 1.01e-20 87.8
MsaG013879 AT1G69310.4 44.444 81 44 1 237 317 124 203 4.29e-20 90.9
MsaG013879 AT1G69310.2 44.444 81 44 1 237 317 124 203 4.29e-20 90.9
MsaG013879 AT1G69310.1 44.444 81 44 1 237 317 124 203 4.29e-20 90.9
MsaG013879 AT5G13080.1 53.425 73 33 1 247 319 54 125 6.24e-20 86.7
MsaG013879 AT3G62340.1 53.247 77 35 1 244 320 102 177 2.19e-19 88.6
MsaG013879 AT4G30935.1 49.351 77 38 1 244 320 315 390 5.89e-19 89.7
MsaG013879 AT1G55600.1 47.436 78 40 1 244 321 291 367 8.92e-19 89.4
MsaG013879 AT2G23320.1 57.627 59 25 0 261 319 241 299 1.49e-18 87.0
MsaG013879 AT4G26440.1 46.753 77 40 1 244 320 356 431 1.78e-18 88.6
MsaG013879 AT1G29280.1 35.115 131 63 2 208 323 14 137 1.78e-18 85.5
MsaG013879 AT5G28650.1 53.968 63 29 0 256 318 258 320 2.90e-18 86.3
MsaG013879 AT4G24240.1 48.148 81 41 1 240 319 260 340 3.13e-18 86.3
MsaG013879 AT2G44745.1 45.333 75 40 1 243 317 128 201 3.67e-18 83.6
MsaG013879 AT2G30590.1 45.122 82 41 1 245 322 294 375 4.04e-18 86.3
MsaG013879 AT2G34830.1 41.748 103 59 1 226 327 180 282 5.69e-18 86.3
MsaG013879 AT1G30650.1 41.748 103 59 1 226 327 182 284 6.35e-18 86.3
MsaG013879 AT2G34830.2 41.748 103 59 1 226 327 189 291 7.01e-18 86.3
MsaG013879 AT4G23550.1 44.444 81 45 0 255 335 135 215 7.03e-18 84.7
MsaG013879 AT4G39410.1 44.737 76 41 1 243 318 206 280 7.91e-18 84.3
MsaG013879 AT5G64810.1 46.479 71 37 1 247 317 97 166 1.32e-17 81.3
MsaG013879 AT3G04670.1 52.381 63 30 0 256 318 258 320 1.37e-17 84.3
MsaG013879 AT2G37260.2 42.105 95 45 3 235 326 248 335 1.52e-17 84.3
MsaG013879 AT2G37260.2 46.667 75 36 3 261 333 84 156 2.54e-14 74.7
MsaG013879 AT2G37260.3 42.105 95 45 3 235 326 250 337 1.63e-17 84.3
MsaG013879 AT2G37260.3 46.667 75 36 3 261 333 86 158 2.58e-14 74.7
MsaG013879 AT2G37260.1 42.105 95 45 3 235 326 330 417 2.47e-17 84.3
MsaG013879 AT2G37260.1 46.667 75 36 3 261 333 166 238 3.67e-14 74.7
MsaG013879 AT2G46130.2 41.837 98 49 3 233 330 3 92 2.79e-17 77.8
MsaG013879 AT3G58710.1 51.429 70 34 0 251 320 62 131 2.95e-17 82.4
MsaG013879 AT3G58710.2 51.429 70 34 0 251 320 61 130 3.28e-17 82.0
MsaG013879 AT4G01250.1 38.095 105 52 1 260 351 128 232 4.04e-17 82.4
MsaG013879 AT4G31550.1 44.737 76 42 0 242 317 228 303 4.33e-17 82.8
MsaG013879 AT4G31550.2 44.737 76 42 0 242 317 227 302 4.41e-17 82.8
MsaG013879 AT2G24570.1 44.737 76 42 0 242 317 225 300 6.18e-17 82.0
MsaG013879 AT5G52830.1 44.156 77 43 0 254 330 159 235 2.94e-16 80.5
MsaG013879 AT4G31800.3 28.788 198 104 5 101 297 52 213 1.58e-15 76.6
MsaG013879 AT1G69310.3 40.741 81 43 2 237 317 124 199 3.80e-15 76.3
MsaG013879 AT5G45260.1 43.434 99 49 3 239 335 1192 1285 2.31e-14 76.6
MsaG013879 AT5G45050.1 43.678 87 38 1 260 335 1180 1266 6.83e-14 75.1
MsaG013879 AT5G45050.4 43.678 87 38 1 260 335 1041 1127 7.30e-14 74.7
MsaG013879 AT5G45050.2 43.678 87 38 1 260 335 1152 1238 7.88e-14 74.7
MsaG013879 AT5G43290.1 40.244 82 47 2 241 321 94 174 9.13e-14 72.0
MsaG013879 AT2G21900.1 42.424 66 37 1 255 320 104 168 4.06e-13 68.6
MsaG013879 AT2G46400.1 43.210 81 44 1 237 315 81 161 3.04e-12 67.8
MsaG013879 AT3G01080.3 50.000 60 28 2 261 320 206 263 1.29e-11 66.2
MsaG013879 AT2G40740.1 42.500 80 42 2 261 336 174 253 4.93e-11 63.9
MsaG013879 AT2G40740.3 42.500 80 42 2 261 336 183 262 5.94e-11 63.9

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGGCCATTCTTGAAAT+TGG 0.104474 3:+62631320 MsaT013879.1:CDS
ACTATGCTTTATTGCAATTC+TGG 0.191922 3:-62633226 None:intergenic
CATAAATTTCTGAGCCAAAA+AGG 0.223319 3:-62633024 None:intergenic
GCTGCATAATATGCTCTTCT+AGG 0.248057 3:-62630971 None:intergenic
GCAGCATCAATGTTACTTTC+AGG 0.317662 3:+62632810 MsaT013879.1:CDS
GGATGTCAATGGAGAAAATA+TGG 0.325975 3:+62632155 MsaT013879.1:CDS
CCCAATTTCAAGAATGGCCT+TGG 0.326304 3:-62631321 None:intergenic
AGCAAGATCAGAAGCTAATA+TGG 0.333642 3:+62631605 MsaT013879.1:CDS
GATCCACTTTCTAATATTGT+TGG 0.346118 3:-62632864 None:intergenic
CCAAGGCCATTCTTGAAATT+GGG 0.361311 3:+62631321 MsaT013879.1:CDS
TTAGTAGGACCATTATTGTT+TGG 0.374768 3:-62633188 None:intergenic
AAACCATCTGCATTTGTCAT+TGG 0.377609 3:-62632834 None:intergenic
GCTCCATTTGGTGTAGGGTT+AGG 0.385260 3:-62629985 None:intergenic
AACAATAATGGTCCTACTAA+TGG 0.392733 3:+62633191 MsaT013879.1:CDS
GCAAAACCCAATTTCAAGAA+TGG 0.393070 3:-62631327 None:intergenic
TGTAGGGTTAGGTCATTAAC+TGG 0.394243 3:-62629974 None:intergenic
GTATATACTTGCTTCCGAAC+TGG 0.394378 3:-62632245 None:intergenic
ACTTCCAATGATGGAAGTTA+TGG 0.397710 3:-62633156 None:intergenic
TGCTGAAGATAGAAGTGTTT+TGG 0.402931 3:+62632713 MsaT013879.1:CDS
ACTAGAGCTGAAGTGAATTT+CGG 0.413891 3:-62633128 None:intergenic
TTTGTTGCTAGGAGAAATCT+TGG 0.415907 3:-62630142 None:intergenic
GCTGCCATAACTTCCATCAT+TGG 0.425352 3:+62633152 MsaT013879.1:CDS
AATTCAAATCCAAACAATAA+TGG 0.432073 3:+62633179 MsaT013879.1:CDS
AAGAATTCACCCTTGGAACT+TGG 0.434846 3:-62631521 None:intergenic
ATCATTTGCAATGATGCTAT+AGG 0.437151 3:+62629836 MsaT013879.1:CDS
ATTTGAATTACTTCCAATGA+TGG 0.439445 3:-62633165 None:intergenic
CAAGCAAATAAAGCATTTCA+AGG 0.441399 3:+62631486 MsaT013879.1:CDS
GATTTCTCCTAGCAACAAAC+TGG 0.442717 3:+62630146 MsaT013879.1:CDS
GTTTGAGAGAGCTAATTGAT+AGG 0.446725 3:+62630928 MsaT013879.1:CDS
TCTCCACTTGTACCATTAGT+AGG 0.450332 3:-62633203 None:intergenic
CTATCATCCAGTTTGTTGCT+AGG 0.450663 3:-62630153 None:intergenic
TCTAATGTGATAGTTGGAAA+TGG 0.453620 3:-62632930 None:intergenic
TGACCTAACCCTACACCAAA+TGG 0.459298 3:+62629982 MsaT013879.1:CDS
TCCAAGGGTGAATTCTTTCA+AGG 0.464827 3:+62631527 MsaT013879.1:CDS
TTATGCAGCTGAAGCATAAA+CGG 0.465240 3:+62630985 MsaT013879.1:CDS
GTGAGATCTAATGTGATAGT+TGG 0.473108 3:-62632936 None:intergenic
CTACGTGATGATGCTCCATT+TGG 0.485074 3:-62629997 None:intergenic
GTGCAACGATAATAAGCTCT+TGG 0.486157 3:-62632206 None:intergenic
CCAAGGGTGAATTCTTTCAA+GGG 0.492785 3:+62631528 MsaT013879.1:CDS
TGGGGACTGCTTGTCCAGTT+CGG 0.500788 3:+62632231 MsaT013879.1:CDS
GAGAAAATATGGACAGAAGA+TGG 0.503010 3:+62632166 MsaT013879.1:CDS
TATGGACAGAAGATGGCAAA+GGG 0.509004 3:+62632173 MsaT013879.1:CDS
CGATAATAAGCTCTTGGACA+TGG 0.509444 3:-62632200 None:intergenic
AATCCAACAATATTAGAAAG+TGG 0.511191 3:+62632861 MsaT013879.1:CDS
TATACAAGAGAATACTGAAA+AGG 0.513323 3:+62631440 MsaT013879.1:CDS
GGCTTTCAAACCAAGTTCCA+AGG 0.521439 3:+62631511 MsaT013879.1:CDS
CCCTTGAAAGAATTCACCCT+TGG 0.522368 3:-62631528 None:intergenic
GCTTATTATCGTTGCACCAT+GGG 0.524789 3:+62632212 MsaT013879.1:CDS
GATGATGCTCCATTTGGTGT+AGG 0.540710 3:-62629991 None:intergenic
TTGCTGTTGGTGGTAGAGCA+TGG 0.541988 3:-62632761 None:intergenic
ATGATGCTCCATTTGGTGTA+GGG 0.544067 3:-62629990 None:intergenic
AGATTTCAGATGGATGTCAA+TGG 0.544482 3:+62632144 MsaT013879.1:intron
AATGTATCACAGATTTCAGA+TGG 0.548456 3:+62632134 MsaT013879.1:intron
AGCTTATTATCGTTGCACCA+TGG 0.549292 3:+62632211 MsaT013879.1:CDS
CTGCATAATATGCTCTTCTA+GGG 0.557538 3:-62630970 None:intergenic
ATGGTATGAATATGATTCCA+AGG 0.557677 3:+62631304 MsaT013879.1:CDS
CAAATAAAGCATTTCAAGGG+TGG 0.566998 3:+62631490 MsaT013879.1:CDS
GGTCCTACTAATGGTACAAG+TGG 0.573879 3:+62633200 MsaT013879.1:CDS
GAAGCAACTGAAGAAAAGAA+TGG 0.574998 3:+62631285 MsaT013879.1:CDS
GTGTATACAGTATGCTGCTG+TGG 0.578588 3:+62630869 MsaT013879.1:intron
TGATGCAAGGAACGAAAATG+AGG 0.583927 3:+62630185 MsaT013879.1:CDS
ATAGAGAATATTCTGATGCA+AGG 0.587268 3:+62630172 MsaT013879.1:CDS
AACTGGACAAGCAGTCCCCA+TGG 0.587671 3:-62632228 None:intergenic
GTTGCCATGGTGTGTGAACA+AGG 0.603956 3:-62632891 None:intergenic
GCTTTCAAACCAAGTTCCAA+GGG 0.616257 3:+62631512 MsaT013879.1:CDS
AAGCAAATAAAGCATTTCAA+GGG 0.619803 3:+62631487 MsaT013879.1:CDS
GGACCAATGACAAATGCAGA+TGG 0.622854 3:+62632831 MsaT013879.1:CDS
GCATAAACGGAGTAAACTTG+AGG 0.635185 3:+62630998 MsaT013879.1:CDS
TGAAGCTGAGAGTGTTGCCA+TGG 0.636245 3:-62632904 None:intergenic
ACCACCAACAGCAAAAGCAA+TGG 0.641019 3:+62632770 MsaT013879.1:CDS
ATATGGACAGAAGATGGCAA+AGG 0.645418 3:+62632172 MsaT013879.1:CDS
ACTCCCTTGTTCACACACCA+TGG 0.651056 3:+62632887 MsaT013879.1:CDS
CTTATTATCGTTGCACCATG+GGG 0.651170 3:+62632213 MsaT013879.1:CDS
ATGGACAGAAGATGGCAAAG+GGG 0.709928 3:+62632174 MsaT013879.1:CDS
TTGCCATGGTGTGTGAACAA+GGG 0.728086 3:-62632890 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 62629824 62633247 62629824 ID=MsaG013879
Chr3 mRNA 62629824 62633247 62629824 ID=MsaT013879.1;Parent=MsaG013879
Chr3 exon 62629824 62630021 62629824 ID=MsaT013879.1.exon1;Parent=MsaT013879.1
Chr3 CDS 62629824 62630021 62629824 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Chr3 exon 62630123 62630206 62630123 ID=MsaT013879.1.exon2;Parent=MsaT013879.1
Chr3 CDS 62630123 62630206 62630123 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Chr3 exon 62630879 62631019 62630879 ID=MsaT013879.1.exon3;Parent=MsaT013879.1
Chr3 CDS 62630879 62631019 62630879 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Chr3 exon 62631276 62631626 62631276 ID=MsaT013879.1.exon4;Parent=MsaT013879.1
Chr3 CDS 62631276 62631626 62631276 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Chr3 exon 62632146 62632259 62632146 ID=MsaT013879.1.exon5;Parent=MsaT013879.1
Chr3 CDS 62632146 62632259 62632146 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Chr3 exon 62632702 62633247 62632702 ID=MsaT013879.1.exon6;Parent=MsaT013879.1
Chr3 CDS 62632702 62633247 62632702 ID=cds.MsaT013879.1;Parent=MsaT013879.1
Gene Sequence

>MsaT013879.1

ATGGAAGAAGAAATCATTTGCAATGATGCTATAGGAAATTCTAGTGTTGTTGAAGTTCCACATTCTTCTTCTTCTTCTCAAGTCATGTCTTTTAATGGAAAAAGACTAATTATGGATGAGGTTGATTTCTTTGCAGATAAGAAAATGAGTCCAGTTAATGACCTAACCCTACACCAAATGGAGCATCATCACGTAGATACTAGTTTAGATCTTCTCACCAAGATTTCTCCTAGCAACAAACTGGATGATAGAGAATATTCTGATGCAAGGAACGAAAATGAGTATGCTGCTGTGGTAGCTAAATTGCACCAAACGAATGCTGAAAATCAACGTTTGAGAGAGCTAATTGATAGGCTTAGAATTGATCACAATGCCCTAGAAGAGCATATTATGCAGCTGAAGCATAAACGGAGTAAACTTGAGATTAATGATGAAGCAACTGAAGAAAAGAATGGTATGAATATGATTCCAAGGCCATTCTTGAAATTGGGTTTTGCTAAAGGTGAGCCTTCACAAGAGAGAAAAAACATGGTTGACTTGATGGAATGTGATAAAAAGAGTATTCAAAAGTTTTACAACAATGGAATTATACAAGAGAATACTGAAAAGGAAGAAGATCATCATGAGAATTCACAAGCAAATAAAGCATTTCAAGGGTGGCTTTCAAACCAAGTTCCAAGGGTGAATTCTTTCAAGGGTGTTGATGATCAAGCATCAGAATCAATGATTAAGAAAGCTCGTGTCTCAGTTAGAGCAAGATCAGAAGCTAATATGATTTCAGATGGATGTCAATGGAGAAAATATGGACAGAAGATGGCAAAGGGGAATCCATGTCCAAGAGCTTATTATCGTTGCACCATGGGGACTGCTTGTCCAGTTCGGAAGCAAGTTCAAAGATGTGCTGAAGATAGAAGTGTTTTGGTAACAACATATGAAGGACAACATAACCATGCTCTACCACCAACAGCAAAAGCAATGGCATCAACAACAACATCAGCAGCATCAATGTTACTTTCAGGACCAATGACAAATGCAGATGGTTTAATAAATCCAACAATATTAGAAAGTGGATCACTCCCTTGTTCACACACCATGGCAACACTCTCAGCTTCAGCTCCATTTCCAACTATCACATTAGATCTCACTGAAGAAGCTATCAATAATTCCTCACAAAAACAACTACAGCAAGGACAGTTCAACCTTCTCCAACCCTTTTTGGCTCAGAAATTTATGTCAGCTCCGAATATTTTTGGAATGGAAAATGCTTCATTTGCTGATACAGTTAGTGCTGCAACAGCTGCTATGACTTCTGATCCGAAATTCACTTCAGCTCTAGTTGCTGCCATAACTTCCATCATTGGAAGTAATTCAAATCCAAACAATAATGGTCCTACTAATGGTACAAGTGGAGACCAGAATTGCAATAAAGCATAG
Protein sequence

>MsaT013879.1

MEEEIICNDAIGNSSVVEVPHSSSSSQVMSFNGKRLIMDEVDFFADKKMSPVNDLTLHQMEHHHVDTSLDLLTKISPSNKLDDREYSDARNENEYAAVVAKLHQTNAENQRLRELIDRLRIDHNALEEHIMQLKHKRSKLEINDEATEEKNGMNMIPRPFLKLGFAKGEPSQERKNMVDLMECDKKSIQKFYNNGIIQENTEKEEDHHENSQANKAFQGWLSNQVPRVNSFKGVDDQASESMIKKARVSVRARSEANMISDGCQWRKYGQKMAKGNPCPRAYYRCTMGTACPVRKQVQRCAEDRSVLVTTYEGQHNHALPPTAKAMASTTTSAASMLLSGPMTNADGLINPTILESGSLPCSHTMATLSASAPFPTITLDLTEEAINNSSQKQLQQGQFNLLQPFLAQKFMSAPNIFGMENASFADTVSAATAAMTSDPKFTSALVAAITSIIGSNSNPNNNGPTNGTSGDQNCNKA*