AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG013904


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013904 MtrunA17_Chr3g0099881 97.849 465 8 2 1 465 1 463 0.0 933
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG013904 AT2G24830.1 54.412 476 191 7 1 462 1 464 7.67e-156 453

Find 117 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACAGGGTTTAGGATTTA+TGG 0.135876 3:+62994543 MsaT013904.1:CDS
TTCCAAAATGATGGCGAATA+TGG 0.222774 3:+62994645 MsaT013904.1:CDS
ACCCATATTCGCCATCATTT+TGG 0.227507 3:-62994647 None:intergenic
AGGAATATGCCCCAACAATT+TGG 0.235755 3:+62994275 MsaT013904.1:CDS
ATAATAATCTCGCACGCTTT+AGG 0.280179 3:-62990842 None:intergenic
GGAACTTGTTCAGGCAATTA+AGG 0.282463 3:+62990797 MsaT013904.1:CDS
AGCCTAAACCCATTCCTTCT+TGG 0.291741 3:-62994672 None:intergenic
CAGCCTCAGCCAATGCCTTA+TGG 0.300973 3:-62995145 None:intergenic
GAAGAGGAACCACAGGGTTT+AGG 0.304634 3:+62994535 MsaT013904.1:CDS
ATTTGGAAGCCGTCCCTTGC+TGG 0.320960 3:+62994292 MsaT013904.1:CDS
CCCTTGCTGGGTCAAGCATA+TGG 0.330283 3:+62994305 MsaT013904.1:CDS
GGAATGGGTTTAGGCTTAAC+TGG 0.336817 3:+62994679 MsaT013904.1:CDS
CTTCGCAAATATAGCAGTTT+CGG 0.344490 3:-62994596 None:intergenic
TTCCTCTGAGCCGCTGTAAT+CGG 0.364138 3:-62994515 None:intergenic
TTGTTGGGGCATATTCCTTA+AGG 0.375669 3:-62994270 None:intergenic
AGACTTAAGTGCATGATCTA+AGG 0.378035 3:-62994752 None:intergenic
TGTTCAACTGACTTCCCTTA+AGG 0.378203 3:+62994255 MsaT013904.1:CDS
GGGACGGCTTCCAAATTGTT+GGG 0.381328 3:-62994285 None:intergenic
TCAGCCAATGCCTTATGGTT+TGG 0.381872 3:-62995140 None:intergenic
GGTTCACGAGGAACTTGTTC+AGG 0.382347 3:+62990788 MsaT013904.1:intron
AGGTTCAGGTGAAGGTAAAA+AGG 0.390687 3:+62994984 MsaT013904.1:CDS
GGTTCTAAAATTTGAACAAA+TGG 0.394751 3:+62995062 MsaT013904.1:CDS
TTCATAGCAGCATCATAAAC+AGG 0.396986 3:-62995108 None:intergenic
TACCTCGAGGAGTTCGGAAT+TGG 0.397854 3:-62989292 None:intergenic
GGGTTTAGGCTTAACTGGTC+AGG 0.398564 3:+62994684 MsaT013904.1:CDS
TACCAAGAAGGAATGGGTTT+AGG 0.400375 3:+62994670 MsaT013904.1:CDS
TTACAACAACGATGTCGATT+TGG 0.412824 3:+62993143 MsaT013904.1:CDS
CTTCTTCATTGATCCATTGT+TGG 0.414052 3:-62994950 None:intergenic
AGGCTTAACTGGTCAGGGTA+TGG 0.427130 3:+62994690 MsaT013904.1:CDS
TCACCTGAACCTTTGCTTTG+CGG 0.427692 3:-62994973 None:intergenic
CAAATGCAAATGCAGGCATT+TGG 0.433219 3:+62994335 MsaT013904.1:CDS
GAAGCTTGGTGCACAAGATA+TGG 0.438563 3:+62994435 MsaT013904.1:CDS
TGGAAGCCATGCCTCTGGTA+TGG 0.440011 3:-62994627 None:intergenic
TGTTGGGGCATATTCCTTAA+GGG 0.440974 3:-62994269 None:intergenic
TGGTAGAACCTTCACTGGTA+TGG 0.441015 3:-62994719 None:intergenic
CCAGAGGTGGTAAGAGAAAA+CGG 0.441799 3:+62994818 MsaT013904.1:CDS
TCCAAAATGATGGCGAATAT+GGG 0.445234 3:+62994646 MsaT013904.1:CDS
TGAAGATACCTCGAGGAGTT+CGG 0.446790 3:-62989298 None:intergenic
TTTATTAGCGAGAAGACATC+TGG 0.451439 3:-62994898 None:intergenic
AGGGACGGCTTCCAAATTGT+TGG 0.452556 3:-62994286 None:intergenic
TGTAGATTCCGTTACAAAGA+TGG 0.463502 3:+62990990 MsaT013904.1:CDS
TTCGTCCCATGACTCAAGCT+CGG 0.469021 3:-62994365 None:intergenic
CAGAAAAGCAAAGGAAGAAA+CGG 0.469810 3:+62994794 MsaT013904.1:CDS
AGCAGCCGAGCTTGAGTCAT+GGG 0.474748 3:+62994360 MsaT013904.1:CDS
CAATGGTCAAGTGATGCAAT+TGG 0.476631 3:+62991022 MsaT013904.1:CDS
GTTCGGAATTGGTTGGATCA+AGG 0.480602 3:-62989281 None:intergenic
TGCACTTAAGTCTCATAAAG+TGG 0.483692 3:+62994762 MsaT013904.1:CDS
GGCTCTGGTTCAACATCTGC+AGG 0.485074 3:-62990933 None:intergenic
GAGAGTCCTTGAAAACCAAC+TGG 0.486111 3:+62989203 MsaT013904.1:CDS
GCATGATCTAAGGACTGCTT+AGG 0.491473 3:-62994742 None:intergenic
GCGAATATGGGTTACCAAGA+AGG 0.492625 3:+62994658 MsaT013904.1:CDS
AAGCCGCAAAGCAAAGGTTC+AGG 0.493600 3:+62994970 MsaT013904.1:CDS
GCACAGCACTGTGTCTGCTT+CGG 0.493677 3:-62990865 None:intergenic
ATGGGTTACCAAGAAGGAAT+GGG 0.495197 3:+62994664 MsaT013904.1:CDS
TTTGGAAGCCGTCCCTTGCT+GGG 0.503809 3:+62994293 MsaT013904.1:CDS
AACTGCTATATTTGCGAAGT+GGG 0.506208 3:+62994600 MsaT013904.1:CDS
CCTTGCTGGGTCAAGCATAT+GGG 0.512367 3:+62994306 MsaT013904.1:CDS
GTAATCGGATTGTTCTAAGC+TGG 0.518452 3:-62994500 None:intergenic
CAGAGGTGGTAAGAGAAAAC+GGG 0.519218 3:+62994819 MsaT013904.1:CDS
TATGGGTTACCAAGAAGGAA+TGG 0.519292 3:+62994663 MsaT013904.1:CDS
CTTAAGTCTCATAAAGTGGA+AGG 0.523148 3:+62994766 MsaT013904.1:CDS
TCCTACATCTGAAAATATGC+TGG 0.524946 3:+62991076 MsaT013904.1:CDS
CTCAGAGGAAGAGGAACCAC+AGG 0.529152 3:+62994528 MsaT013904.1:CDS
TTGAACGCCTTTCTGTAAGT+TGG 0.529456 3:-62994572 None:intergenic
CCCATATGCTTGACCCAGCA+AGG 0.531582 3:-62994306 None:intergenic
GGTAGAACCTTCACTGGTAT+GGG 0.533043 3:-62994718 None:intergenic
TTACAGCGGCTCAGAGGAAG+AGG 0.535906 3:+62994519 MsaT013904.1:CDS
AGGCATGGCTTCCAAAATGA+TGG 0.538766 3:+62994636 MsaT013904.1:CDS
CATGTAATTGAAGTTCCAGT+TGG 0.543818 3:-62989218 None:intergenic
GCAATTAAGGATGCCGAGGA+AGG 0.547779 3:+62990810 MsaT013904.1:CDS
TCCGTTACAAAGATGGGCGT+TGG 0.550166 3:+62990997 MsaT013904.1:CDS
CACGGTGAAGCTTCCAACAA+TGG 0.551415 3:+62994937 MsaT013904.1:CDS
AAACCATAAGGCATTGGCTG+AGG 0.552203 3:+62995142 MsaT013904.1:CDS
GTAGATTCCGTTACAAAGAT+GGG 0.552313 3:+62990991 MsaT013904.1:CDS
GTTGGAAGCTTCACCGTGCA+TGG 0.554167 3:-62994932 None:intergenic
AAAGATGGGCGTTGGTACAA+TGG 0.554183 3:+62991005 MsaT013904.1:CDS
GGTTTAGGCTTAACTGGTCA+GGG 0.554398 3:+62994685 MsaT013904.1:CDS
AACAACATCATCTGAGGCAG+AGG 0.558032 3:-62995180 None:intergenic
TCGAGGAGTTCGGAATTGGT+TGG 0.566915 3:-62989288 None:intergenic
CAAAGCAAAGGTTCAGGTGA+AGG 0.567290 3:+62994976 MsaT013904.1:CDS
TTAGCAGCTTACCATGGGAT+AGG 0.568857 3:-62993178 None:intergenic
ATGAAGAAGCCGCAAAGCAA+AGG 0.572676 3:+62994964 MsaT013904.1:CDS
GAGCAGCCGAGCTTGAGTCA+TGG 0.572843 3:+62994359 MsaT013904.1:CDS
GCAGTCTCAAATGCAAATGC+AGG 0.576110 3:+62994328 MsaT013904.1:CDS
TTAGGTGGTAGAACCTTCAC+TGG 0.576337 3:-62994724 None:intergenic
TCGCAGAACTTCCATCATCT+CGG 0.576538 3:-62994414 None:intergenic
TAGTCCAAACCATAAGGCAT+TGG 0.577022 3:+62995136 MsaT013904.1:CDS
GATAGTACCAACTTACAGAA+AGG 0.577624 3:+62994565 MsaT013904.1:CDS
TGTGAAGGATTTGAAAGTGC+AGG 0.591216 3:+62995041 MsaT013904.1:CDS
TCAACATCTGCAGGATCAAG+TGG 0.594691 3:-62990924 None:intergenic
GATGGAAGTTCTGCGAAGCT+TGG 0.595918 3:+62994421 MsaT013904.1:CDS
TAGGTGAAGAAGTCCTTCCT+CGG 0.599243 3:-62990823 None:intergenic
ACCAACGCCCATCTTTGTAA+CGG 0.601052 3:-62990998 None:intergenic
GGACGGCTTCCAAATTGTTG+GGG 0.603564 3:-62994284 None:intergenic
AGGAAGAAACGGACCAGAGG+TGG 0.605286 3:+62994805 MsaT013904.1:CDS
TGCTTATGAGAATGATGTGA+AGG 0.609787 3:+62995026 MsaT013904.1:CDS
TGATCTAAGGACTGCTTAGG+TGG 0.612721 3:-62994739 None:intergenic
AGTAACTGTCGCCTATCCCA+TGG 0.613342 3:+62993167 MsaT013904.1:CDS
CAAAGGAAGAAACGGACCAG+AGG 0.617674 3:+62994802 MsaT013904.1:CDS
GATGCAATTGGATAATTCAG+TGG 0.621983 3:+62991034 MsaT013904.1:CDS
AACACTCAACTAGCCATGCA+CGG 0.622219 3:+62994919 MsaT013904.1:CDS
GGTTGATCCCATACCAGTGA+AGG 0.622390 3:+62994711 MsaT013904.1:CDS
GTCGATATCCCAGGTTCACG+AGG 0.623116 3:+62990776 MsaT013904.1:intron
TTTGCTAACAACATCATCTG+AGG 0.624235 3:-62995186 None:intergenic
ATGCTTGACCCAGCAAGGGA+CGG 0.625116 3:-62994301 None:intergenic
AAACTGCTATATTTGCGAAG+TGG 0.626176 3:+62994599 MsaT013904.1:CDS
GGAGAACCATACCAGAGGCA+TGG 0.631835 3:+62994621 MsaT013904.1:CDS
TTAAGTCTCATAAAGTGGAA+GGG 0.632685 3:+62994767 MsaT013904.1:CDS
CCATATGCTTGACCCAGCAA+GGG 0.636597 3:-62994305 None:intergenic
ATCCGATTACAGCGGCTCAG+AGG 0.638535 3:+62994513 MsaT013904.1:CDS
AAGGGAACACAGAAAAGCAA+AGG 0.640836 3:+62994785 MsaT013904.1:CDS
TCAGGCAATTAAGGATGCCG+AGG 0.649742 3:+62990806 MsaT013904.1:CDS
AACCAATTCCGAACTCCTCG+AGG 0.659085 3:+62989290 MsaT013904.1:CDS
TCCATAAATCCTAAACCCTG+TGG 0.699072 3:-62994544 None:intergenic
AAGTGGGAGAACCATACCAG+AGG 0.724288 3:+62994616 MsaT013904.1:CDS
TTAGAACAATCCGATTACAG+CGG 0.729255 3:+62994505 MsaT013904.1:CDS
TCAGAGGAAGAGGAACCACA+GGG 0.729850 3:+62994529 MsaT013904.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 62989186 62995242 62989186 ID=MsaG013904
Chr3 mRNA 62989186 62995242 62989186 ID=MsaT013904.1;Parent=MsaG013904
Chr3 exon 62989186 62989311 62989186 ID=MsaT013904.1.exon1;Parent=MsaT013904.1
Chr3 CDS 62989186 62989311 62989186 ID=cds.MsaT013904.1;Parent=MsaT013904.1
Chr3 exon 62990789 62991097 62990789 ID=MsaT013904.1.exon2;Parent=MsaT013904.1
Chr3 CDS 62990789 62991097 62990789 ID=cds.MsaT013904.1;Parent=MsaT013904.1
Chr3 exon 62993128 62993188 62993128 ID=MsaT013904.1.exon3;Parent=MsaT013904.1
Chr3 CDS 62993128 62993188 62993128 ID=cds.MsaT013904.1;Parent=MsaT013904.1
Chr3 exon 62994248 62995242 62994248 ID=MsaT013904.1.exon4;Parent=MsaT013904.1
Chr3 CDS 62994248 62995242 62994248 ID=cds.MsaT013904.1;Parent=MsaT013904.1
Gene Sequence

>MsaT013904.1

ATGGGTAGCGAAGAAGAGAGAGTCCTTGAAAACCAACTGGAACTTCAATTACATGAACAAAGAGATTCTCTCACTGCTATCGATCATGCTCTTCTCCTTGATCCAACCAATTCCGAACTCCTCGAGGTTCACGAGGAACTTGTTCAGGCAATTAAGGATGCCGAGGAAGGACTTCTTCACCTAAAGCGTGCGAGATTATTATCCGAAGCAGACACAGTGCTGTGCAGTACTGATATTTTTGCTGAAGAAAAGAAAGTGGAGCCACTTGATCCTGCAGATGTTGAACCAGAGCCGCTAGAAGAAAAAAGCTTTTCTATTGGATCTAAATGTAGATTCCGTTACAAAGATGGGCGTTGGTACAATGGTCAAGTGATGCAATTGGATAATTCAGTGGCGAAGATTTCATTTCTTACTCCTACATCTGAAAATATGCTGATGTGCAAGTTCTTTTTACAACAACGATGTCGATTTGGTAGTAACTGTCGCCTATCCCATGGAGTTGATGTTCAACTGACTTCCCTTAAGGAATATGCCCCAACAATTTGGAAGCCGTCCCTTGCTGGGTCAAGCATATGGGCAGTCTCAAATGCAAATGCAGGCATTTGGAGAGCAGCCGAGCTTGAGTCATGGGACGAAAAAGCTGGAGTTGGACAAGTTGTTTTCCGAGATGATGGAAGTTCTGCGAAGCTTGGTGCACAAGATATGGTATTATCTGAGTATGCAGATATGAGTGACATAGATAGTGATTCCAGCTTAGAACAATCCGATTACAGCGGCTCAGAGGAAGAGGAACCACAGGGTTTAGGATTTATGGATAGTACCAACTTACAGAAAGGCGTTCAAACCGAAACTGCTATATTTGCGAAGTGGGAGAACCATACCAGAGGCATGGCTTCCAAAATGATGGCGAATATGGGTTACCAAGAAGGAATGGGTTTAGGCTTAACTGGTCAGGGTATGGTTGATCCCATACCAGTGAAGGTTCTACCACCTAAGCAGTCCTTAGATCATGCACTTAAGTCTCATAAAGTGGAAGGGAACACAGAAAAGCAAAGGAAGAAACGGACCAGAGGTGGTAAGAGAAAACGGGAGAAGAGACATGCAGCTGCAATTCGTGCTGCAAAAGAAGAAGAAGAAGAATTGACACCAGATGTCTTCTCGCTAATAAACACTCAACTAGCCATGCACGGTGAAGCTTCCAACAATGGATCAATGAAGAAGCCGCAAAGCAAAGGTTCAGGTGAAGGTAAAAAGGTGGATAGAAAAATGTTGGTTGCTTATGAGAATGATGTGAAGGATTTGAAAGTGCAGGTTCTAAAATTTGAACAAATGGCAGAAGCAAATAAAAGAGAAAAACCTGTTTATGATGCTGCTATGAAAAAATTAGTCCAAACCATAAGGCATTGGCTGAGGCTGAAGCAGTTCATGCCTCTGCCTCAGATGATGTTGTTAGCAAAGAAAAAGATAAGAGATGGCTTAAGTTTTAGTTAG

Protein sequence

>MsaT013904.1

MGSEEERVLENQLELQLHEQRDSLTAIDHALLLDPTNSELLEVHEELVQAIKDAEEGLLHLKRARLLSEADTVLCSTDIFAEEKKVEPLDPADVEPEPLEEKSFSIGSKCRFRYKDGRWYNGQVMQLDNSVAKISFLTPTSENMLMCKFFLQQRCRFGSNCRLSHGVDVQLTSLKEYAPTIWKPSLAGSSIWAVSNANAGIWRAAELESWDEKAGVGQVVFRDDGSSAKLGAQDMVLSEYADMSDIDSDSSLEQSDYSGSEEEEPQGLGFMDSTNLQKGVQTETAIFAKWENHTRGMASKMMANMGYQEGMGLGLTGQGMVDPIPVKVLPPKQSLDHALKSHKVEGNTEKQRKKRTRGGKRKREKRHAAAIRAAKEEEEELTPDVFSLINTQLAMHGEASNNGSMKKPQSKGSGEGKKVDRKMLVAYENDVKDLKVQVLKFEQMAEANKREKPVYDAAMKKLVQTIRHWLRLKQFMPLPQMMLLAKKKIRDGLSFS*