AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014043


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014043 MtrunA17_Chr7g0225201 82.192 73 12 1 14 86 26 97 1.89e-37 127
MsaG014043 MtrunA17_Chr3g0132681 83.784 74 10 2 14 86 25 97 1.29e-35 122
MsaG014043 MtrunA17_Chr3g0101111 78.082 73 15 1 14 86 25 96 2.53e-35 121
MsaG014043 MtrunA17_Chr3g0101091 78.082 73 15 1 14 86 25 96 2.66e-35 121
MsaG014043 MtrunA17_Chr3g0101081 78.082 73 15 1 14 86 25 96 2.66e-35 121
MsaG014043 MtrunA17_Chr5g0445271 71.233 73 20 1 14 86 26 97 1.70e-30 109
MsaG014043 MtrunA17_Chr5g0447731 67.123 73 23 1 14 86 22 93 1.18e-28 103
MsaG014043 MtrunA17_Chr1g0190951 53.425 73 33 1 14 86 23 94 5.57e-22 88.2
MsaG014043 MtrunA17_Chr1g0205471 53.425 73 33 1 14 86 23 94 1.07e-21 87.4
MsaG014043 MtrunA17_Chr3g0116811 54.795 73 32 1 14 86 25 96 1.83e-21 86.7
MsaG014043 MtrunA17_Chr7g0273851 50.685 73 35 1 14 86 23 94 6.33e-21 85.5
MsaG014043 MtrunA17_Chr3g0119911 50.667 75 36 1 12 86 17 90 2.03e-20 84.0
MsaG014043 MtrunA17_Chr7g0244601 47.297 74 38 1 14 87 27 99 2.61e-18 77.8
MsaG014043 MtrunA17_Chr3g0129771 49.315 73 36 1 14 86 32 103 2.16e-16 72.4
MsaG014043 MtrunA17_Chr5g0432541 47.297 74 38 1 14 87 26 98 3.96e-16 71.6
MsaG014043 MtrunA17_Chr4g0013051 43.836 73 40 1 14 86 23 94 6.23e-16 71.2
MsaG014043 MtrunA17_Chr8g0346511 46.575 73 38 1 14 86 37 108 1.31e-15 70.1
MsaG014043 MtrunA17_Chr8g0384001 42.466 73 41 1 14 86 27 98 7.67e-14 65.1
MsaG014043 MtrunA17_Chr6g0455931 45.333 75 39 1 15 87 26 100 1.91e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014043 AT5G55560.1 71.233 73 20 1 14 86 30 101 5.15e-30 108
MsaG014043 AT3G04910.1 54.054 74 33 1 14 87 23 95 9.52e-21 85.1
MsaG014043 AT5G28080.2 51.351 74 35 1 14 87 24 96 1.76e-19 81.6
MsaG014043 AT3G22420.6 47.297 74 38 1 14 87 23 95 2.12e-19 81.3
MsaG014043 AT3G22420.4 47.297 74 38 1 14 87 94 166 2.13e-19 81.3
MsaG014043 AT3G22420.3 47.297 74 38 1 14 87 94 166 2.31e-19 81.3
MsaG014043 AT3G22420.1 47.297 74 38 1 14 87 23 95 2.39e-19 81.3
MsaG014043 AT3G22420.2 47.297 74 38 1 14 87 23 95 2.42e-19 81.3
MsaG014043 AT3G22420.5 47.297 74 38 1 14 87 23 95 2.45e-19 81.3
MsaG014043 AT3G48260.1 56.164 73 31 1 14 86 21 92 3.74e-19 80.5
MsaG014043 AT3G18750.1 49.333 75 37 1 12 86 25 98 5.37e-19 80.1
MsaG014043 AT3G18750.3 49.333 75 37 1 12 86 25 98 5.37e-19 80.1
MsaG014043 AT3G18750.5 49.333 75 37 1 12 86 25 98 5.37e-19 80.1
MsaG014043 AT3G18750.6 49.333 75 37 1 12 86 25 98 6.47e-19 80.1
MsaG014043 AT3G18750.2 49.333 75 37 1 12 86 25 98 6.47e-19 80.1
MsaG014043 AT1G49160.2 49.315 73 36 1 14 86 27 98 8.72e-19 79.7
MsaG014043 AT1G64630.1 53.425 73 33 1 14 86 15 86 4.34e-17 74.7
MsaG014043 AT3G18750.4 50.000 66 33 0 21 86 15 80 6.33e-17 74.3
MsaG014043 AT3G51630.1 48.684 76 38 1 14 89 24 98 7.39e-17 73.9
MsaG014043 AT3G51630.2 48.684 76 38 1 14 89 24 98 7.39e-17 73.9
MsaG014043 AT5G41990.1 49.315 73 36 1 14 86 28 99 3.29e-16 72.4
MsaG014043 AT5G58350.1 48.000 75 38 1 14 88 18 91 3.91e-15 69.3
MsaG014043 AT3G04910.2 48.333 60 31 0 28 87 13 72 9.59e-15 68.2
MsaG014043 AT1G49160.3 50.000 56 28 0 31 86 90 145 1.28e-14 67.8
MsaG014043 AT1G49160.5 50.000 56 28 0 31 86 90 145 1.48e-14 67.4
MsaG014043 AT1G49160.1 50.000 56 28 0 31 86 25 80 8.88e-14 65.5

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTGCCCTTTACTCTGTT+TGG 0.345633 3:+65904665 MsaT014043.1:CDS
AGATACAACGAGCTACTTCT+AGG 0.410049 3:+65904481 MsaT014043.1:CDS
GTTGGGTCAGTTTCAACAAA+TGG 0.425199 3:-65904447 None:intergenic
ATCCCTCCAAACAGAGTAAA+GGG 0.426117 3:-65904671 None:intergenic
AAACTGACCCAACTGGAATA+TGG 0.426612 3:+65904457 MsaT014043.1:CDS
TTTGTTGAAACTGACCCAAC+TGG 0.432692 3:+65904450 MsaT014043.1:CDS
ATGACCCCGCAATGATTGAT+AGG 0.452773 3:+65904596 MsaT014043.1:CDS
TTGCCCTTTACTCTGTTTGG+AGG 0.460477 3:+65904668 MsaT014043.1:CDS
TGCCCTTTACTCTGTTTGGA+GGG 0.474929 3:+65904669 MsaT014043.1:CDS
TAAAGCCTATCAATCATTGC+GGG 0.480012 3:-65904601 None:intergenic
TGTATCTACCATATTCCAGT+TGG 0.490645 3:-65904465 None:intergenic
AACGAGCTACTTCTAGGATC+AGG 0.510164 3:+65904487 MsaT014043.1:CDS
CATCCCTCCAAACAGAGTAA+AGG 0.514403 3:-65904672 None:intergenic
GAGAAGGAATAGAAGTTACA+TGG 0.564796 3:+65904545 MsaT014043.1:CDS
AAAGCATTTGATCAAAGAGA+AGG 0.567617 3:+65904529 MsaT014043.1:CDS
GTATCTACCATATTCCAGTT+GGG 0.576044 3:-65904464 None:intergenic
ACATGGAATCATGTTAAACT+CGG 0.619363 3:+65904562 MsaT014043.1:CDS
TTTGGAGGGATGTTTACAAG+TGG 0.635380 3:+65904683 MsaT014043.1:CDS
GTAAAGCCTATCAATCATTG+CGG 0.684517 3:-65904602 None:intergenic
AAAGCCTATCAATCATTGCG+GGG 0.706697 3:-65904600 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 65904436 65904708 65904436 ID=MsaG014043
Chr3 mRNA 65904436 65904708 65904436 ID=MsaT014043.1;Parent=MsaG014043
Chr3 exon 65904436 65904708 65904436 ID=MsaT014043.1.exon1;Parent=MsaT014043.1
Chr3 CDS 65904436 65904708 65904436 ID=cds.MsaT014043.1;Parent=MsaT014043.1
Gene Sequence

>MsaT014043.1

ATGACTCAGAACCATTTGTTGAAACTGACCCAACTGGAATATGGTAGATACAACGAGCTACTTCTAGGATCAGGTTCTTGTAAAAAAGTGTACAAAGCATTTGATCAAAGAGAAGGAATAGAAGTTACATGGAATCATGTTAAACTCGGAAATTTCAGTAATGACCCCGCAATGATTGATAGGCTTTACTCTGAAGTGAAATTGCTTAGAAGCATGAAAATCAAAAACATCATTGCCCTTTACTCTGTTTGGAGGGATGTTTACAAGTGGTAA

Protein sequence

>MsaT014043.1

MTQNHLLKLTQLEYGRYNELLLGSGSCKKVYKAFDQREGIEVTWNHVKLGNFSNDPAMIDRLYSEVKLLRSMKIKNIIALYSVWRDVYKW*