AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014126


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014126 MtrunA17_Chr7g0262351 68.750 112 19 1 2 97 4 115 5.16e-46 144
MsaG014126 MtrunA17_Chr6g0472261 67.105 76 9 1 11 70 1 76 4.54e-26 92.0
MsaG014126 MtrunA17_Chr8g0364781 47.573 103 35 3 14 97 6 108 1.36e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014126 AT5G26594.1 44.118 102 40 2 13 97 11 112 4.93e-20 79.0
MsaG014126 AT3G04280.1 39.175 97 40 3 20 97 22 118 5.06e-12 58.5
MsaG014126 AT3G04280.2 39.175 97 40 3 20 97 22 118 5.06e-12 58.5
MsaG014126 AT3G04280.3 39.175 97 40 3 20 97 22 118 5.06e-12 58.5

Find 19 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATAAGTGTTGTTAATT+CGG 0.276668 3:+67956954 None:intergenic
TTTAATTATTCTCGTGGATA+AGG 0.314312 3:-67956813 MsaT014126.1:CDS
CTTATGGAACCTCAAAATTC+AGG 0.357949 3:-67956979 MsaT014126.1:CDS
AAACAAGAATTTATGAAAGC+TGG 0.381997 3:-67956552 MsaT014126.1:CDS
ACGGAAAGGGAAAGGGCTTA+TGG 0.383792 3:-67956995 MsaT014126.1:CDS
ACTAAGAAACTGCGAGCAAT+TGG 0.424462 3:-67956621 MsaT014126.1:CDS
AAGGAAATGCCTATCATGAA+TGG 0.455972 3:-67956794 MsaT014126.1:CDS
AAATGGAAGGTCAACGGAAA+GGG 0.471450 3:-67957008 MsaT014126.1:CDS
ACCTCAATCCCATTCATGAT+AGG 0.485829 3:+67956785 None:intergenic
AACAAGAATTTATGAAAGCT+GGG 0.487065 3:-67956551 MsaT014126.1:CDS
GAAGGTCAACGGAAAGGGAA+AGG 0.504074 3:-67957003 MsaT014126.1:CDS
AGGAAATGCCTATCATGAAT+GGG 0.507526 3:-67956793 MsaT014126.1:CDS
GCCTATCATGAATGGGATTG+AGG 0.520152 3:-67956786 MsaT014126.1:intron
GGTATTCGTAGCATGATTGT+TGG 0.523765 3:-67956600 MsaT014126.1:CDS
CTTGTTCGAATGATTCATCA+AGG 0.541897 3:-67956919 MsaT014126.1:CDS
AAGGTCAACGGAAAGGGAAA+GGG 0.548949 3:-67957002 MsaT014126.1:CDS
GGAACTTTGCACAAAATGGA+AGG 0.563063 3:-67957021 None:intergenic
AAAATGGAAGGTCAACGGAA+AGG 0.575882 3:-67957009 MsaT014126.1:CDS
TGCACAAAATGGAAGGTCAA+CGG 0.621446 3:-67957014 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 67956545 67957028 67956545 ID=MsaG014126
Chr3 mRNA 67956545 67957028 67956545 ID=MsaT014126.1;Parent=MsaG014126
Chr3 exon 67956545 67956646 67956545 ID=MsaT014126.1.exon3;Parent=MsaT014126.1
Chr3 CDS 67956545 67956646 67956545 ID=cds.MsaT014126.1;Parent=MsaT014126.1
Chr3 exon 67956787 67956837 67956787 ID=MsaT014126.1.exon2;Parent=MsaT014126.1
Chr3 CDS 67956787 67956837 67956787 ID=cds.MsaT014126.1;Parent=MsaT014126.1
Chr3 exon 67956885 67957028 67956885 ID=MsaT014126.1.exon1;Parent=MsaT014126.1
Chr3 CDS 67956885 67957028 67956885 ID=cds.MsaT014126.1;Parent=MsaT014126.1
Gene Sequence

>MsaT014126.1

ATGGAAGGTCAACGGAAAGGGAAAGGGCTTATGGAACCTCAAAATTCAGGATCCGAATTAACAACACTTATTGTAGATGATGTTAAGCTTGTTCGAATGATTCATCAAGGTCTATTGAAAAGAGCTCGTGTGAAAAATGAAGCTATTTTAATTATTCTCGTGGATAAGGAAATGCCTATCATGAATGGGATTGAGGCAACTAAGAAACTGCGAGCAATTGGTATTCGTAGCATGATTGTTGGTGTGTCGTCGTGTACAGAAACTGAAAAACAAGAATTTATGAAAGCTGGGAAGTAG

Protein sequence

>MsaT014126.1

MEGQRKGKGLMEPQNSGSELTTLIVDDVKLVRMIHQGLLKRARVKNEAILIILVDKEMPIMNGIEATKKLRAIGIRSMIVGVSSCTETEKQEFMKAGK*