AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014136 MtrunA17_Chr3g0102351 98.421 190 2 1 1 189 1 190 5.19e-137 380
MsaG014136 MtrunA17_Chr5g0446491 77.202 193 31 6 1 189 1 184 6.81e-93 268
MsaG014136 MtrunA17_Chr2g0290491 80.328 122 14 1 1 122 1 112 5.08e-66 199
MsaG014136 MtrunA17_Chr7g0263601 73.684 133 24 3 13 135 9 140 1.43e-62 191
MsaG014136 MtrunA17_Chr8g0382931 73.438 128 32 1 23 150 24 149 1.77e-61 189
MsaG014136 MtrunA17_Chr8g0384451 63.504 137 41 1 26 153 39 175 4.24e-60 186
MsaG014136 MtrunA17_Chr4g0076321 71.074 121 27 1 1 121 1 113 3.19e-58 179
MsaG014136 MtrunA17_Chr1g0185911 67.442 129 40 1 1 127 1 129 4.23e-58 180
MsaG014136 MtrunA17_Chr4g0076381 67.647 102 33 0 21 122 52 153 2.80e-50 162
MsaG014136 MtrunA17_Chr1g0195851 71.875 96 27 0 26 121 35 130 3.03e-50 160
MsaG014136 MtrunA17_Chr2g0296321 51.316 152 61 2 26 173 65 207 4.88e-48 157
MsaG014136 MtrunA17_Chr1g0165041 68.421 95 30 0 26 120 5 99 3.64e-47 152
MsaG014136 MtrunA17_Chr4g0067091 68.817 93 29 0 26 118 21 113 3.08e-46 151
MsaG014136 MtrunA17_Chr1g0191981 61.957 92 35 0 27 118 4 95 2.97e-39 130
MsaG014136 MtrunA17_Chr1g0159271 53.750 80 37 0 41 120 1 80 4.11e-24 91.3
MsaG014136 MtrunA17_Chr5g0446601 47.368 95 47 1 24 115 18 112 2.50e-23 91.7
MsaG014136 MtrunA17_Chr1g0159291 43.421 76 43 0 32 107 7 82 1.11e-19 79.3
MsaG014136 MtrunA17_Chr1g0158951 53.125 64 30 0 57 120 19 82 4.31e-19 78.6
MsaG014136 MtrunA17_Chr4g0062271 36.250 80 51 0 28 107 12 91 3.79e-16 72.0
MsaG014136 MtrunA17_Chr2g0307551 36.250 80 51 0 28 107 12 91 4.08e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014136 AT4G14540.1 74.534 161 30 4 1 156 1 155 8.79e-73 217
MsaG014136 AT5G47640.1 83.607 122 20 0 1 122 1 122 5.14e-68 206
MsaG014136 AT2G37060.2 60.241 166 53 4 1 155 1 164 2.28e-60 186
MsaG014136 AT2G37060.3 60.241 166 53 4 1 155 1 164 2.28e-60 186
MsaG014136 AT2G37060.1 60.241 166 53 4 1 155 1 164 2.28e-60 186
MsaG014136 AT3G53340.5 79.592 98 20 0 23 120 25 122 4.53e-57 177
MsaG014136 AT3G53340.1 79.592 98 20 0 23 120 25 122 4.53e-57 177
MsaG014136 AT2G38880.3 71.667 120 28 2 1 120 1 114 5.47e-57 176
MsaG014136 AT2G38880.2 71.667 120 28 2 1 120 1 114 7.59e-57 176
MsaG014136 AT2G38880.1 71.667 120 28 2 1 120 1 114 7.59e-57 176
MsaG014136 AT2G38880.5 71.667 120 28 2 1 120 1 114 7.59e-57 176
MsaG014136 AT3G53340.3 80.208 96 19 0 23 118 25 120 5.08e-56 174
MsaG014136 AT3G53340.2 80.208 96 19 0 23 118 25 120 5.08e-56 174
MsaG014136 AT2G13570.1 81.443 97 18 0 26 122 35 131 1.92e-55 175
MsaG014136 AT3G53340.4 78.947 95 20 0 23 117 25 119 2.52e-55 171
MsaG014136 AT2G38880.4 71.795 117 27 2 1 117 1 111 2.73e-55 171
MsaG014136 AT2G38880.9 71.795 117 27 2 1 117 1 111 5.98e-55 171
MsaG014136 AT2G38880.10 71.795 117 27 2 1 117 1 111 5.98e-55 171
MsaG014136 AT2G38880.6 71.795 117 27 2 1 117 1 111 5.98e-55 171
MsaG014136 AT2G38880.7 72.414 116 26 2 1 116 1 110 6.14e-55 171
MsaG014136 AT2G38880.11 71.795 117 27 2 1 117 1 111 2.03e-54 171
MsaG014136 AT2G38880.8 71.795 117 27 2 1 117 1 111 2.03e-54 171
MsaG014136 AT5G47670.2 52.866 157 69 2 16 167 18 174 1.03e-51 165
MsaG014136 AT5G47670.1 52.866 157 69 2 16 167 47 203 2.62e-51 165
MsaG014136 AT5G47670.3 52.866 157 69 2 16 167 47 203 2.62e-51 165
MsaG014136 AT2G47810.1 71.717 99 28 0 23 121 47 145 1.63e-50 160
MsaG014136 AT1G21970.1 61.386 101 39 0 26 126 58 158 1.98e-45 150
MsaG014136 AT1G09030.1 60.417 96 38 0 27 122 3 98 2.66e-42 139
MsaG014136 AT5G08190.2 35.354 99 62 2 27 123 11 109 8.80e-18 77.0
MsaG014136 AT5G23090.4 33.673 98 63 2 28 123 12 109 3.25e-17 75.1
MsaG014136 AT5G08190.1 38.750 80 49 0 28 107 12 91 4.12e-17 75.1
MsaG014136 AT5G23090.5 37.179 78 49 0 28 105 12 89 1.18e-16 73.9
MsaG014136 AT5G23090.2 37.179 78 49 0 28 105 12 89 1.18e-16 73.9
MsaG014136 AT5G23090.1 37.179 78 49 0 28 105 12 89 1.18e-16 73.9
MsaG014136 AT5G23090.3 37.097 62 39 0 44 105 15 76 3.29e-12 61.6
MsaG014136 AT2G27470.1 34.091 88 53 1 32 114 11 98 1.53e-11 62.0

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGTGGGCCTGGGTATCC+TGG 0.171258 3:+68161878 MsaT014136.1:CDS
CCCCTTAAGAAATGTCTATC+TGG 0.241386 3:-68161924 None:intergenic
GGGCCTGGGTATCCTGGGCC+TGG 0.280888 3:+68161884 MsaT014136.1:CDS
AAGGTTATCTTCAGAGGTTT+AGG 0.283842 3:+68161684 MsaT014136.1:CDS
CTTTGGGCGATGACGACGTT+GGG 0.306849 3:+68161635 MsaT014136.1:CDS
CAACGGCGATGATTTGCTTT+GGG 0.342319 3:+68161619 MsaT014136.1:CDS
CAGGGACACGTGTATGGTTC+TGG 0.350044 3:+68161827 MsaT014136.1:CDS
CTTCTCTCGCTGACACTTAT+CGG 0.356219 3:-68161585 None:intergenic
TGGTGGGTTTCATCAAGTTA+TGG 0.357962 3:+68161853 MsaT014136.1:CDS
TCTTATGAGAGTGGTGGTTA+TGG 0.366998 3:+68161782 MsaT014136.1:CDS
TCAACGGCGATGATTTGCTT+TGG 0.384468 3:+68161618 MsaT014136.1:CDS
AGGGTGGGCCTGGGTATCCT+GGG 0.386249 3:+68161879 MsaT014136.1:CDS
ATCCTGCTTACGTTTGCTAT+TGG 0.386421 3:-68161458 None:intergenic
AGGTTATCTTCAGAGGTTTA+GGG 0.388502 3:+68161685 MsaT014136.1:CDS
CGTTTGCTATTGGTAGAAAT+CGG 0.398071 3:-68161448 None:intergenic
GGTGGGTTTCATCAAGTTAT+GGG 0.401138 3:+68161854 MsaT014136.1:CDS
GGCCTGGGTATCCTGGGCCT+GGG 0.402305 3:+68161885 MsaT014136.1:CDS
GTTATGGGTAAGGGTGGGCC+TGG 0.416967 3:+68161869 MsaT014136.1:CDS
TTTGAGATCTTCGCGTTCGC+TGG 0.427710 3:-68161497 None:intergenic
GTGTATGGTTCTGGTGGTGG+TGG 0.434372 3:+68161836 MsaT014136.1:CDS
AGTGGTGGTTATGGTGGTGG+TGG 0.437086 3:+68161791 MsaT014136.1:CDS
CACGTGTATGGTTCTGGTGG+TGG 0.446845 3:+68161833 MsaT014136.1:CDS
TCCTCCTCCGTGTGGTCCGC+CGG 0.450553 3:-68161384 None:intergenic
GAGAGTGGTGGTTATGGTGG+TGG 0.453972 3:+68161788 MsaT014136.1:CDS
ATGCATCAGGGACACGTGTA+TGG 0.456937 3:+68161821 MsaT014136.1:CDS
CCTGGGCCTGGGTCTAATAC+TGG 0.461983 3:+68161896 MsaT014136.1:CDS
GGAGATGGAAGGAGAGAAGA+CGG 0.462758 3:+68161706 MsaT014136.1:CDS
TCTTCAGAGGTTTAGGGAGA+TGG 0.465400 3:+68161691 MsaT014136.1:CDS
TGGTCCGCCGGAGTCGTTGT+CGG 0.473319 3:-68161372 None:intergenic
TACCAATAGCAAACGTAAGC+AGG 0.482118 3:+68161456 MsaT014136.1:CDS
TGGTGGAGTTATGATGCATC+AGG 0.485483 3:+68161808 MsaT014136.1:CDS
CCCTTAAGAAATGTCTATCT+GGG 0.498089 3:-68161923 None:intergenic
GTTTCATCAAGTTATGGGTA+AGG 0.498474 3:+68161859 MsaT014136.1:CDS
TTATGGGTAAGGGTGGGCCT+GGG 0.517687 3:+68161870 MsaT014136.1:CDS
GATGACGACGTTGGGGTTCG+AGG 0.521900 3:+68161643 MsaT014136.1:CDS
TATGAGAGTGGTGGTTATGG+TGG 0.523250 3:+68161785 MsaT014136.1:CDS
CGTTGAAAGGTTATCTTCAG+AGG 0.526003 3:+68161678 MsaT014136.1:CDS
CGTGATAAAGACGCGCCGCA+AGG 0.530875 3:+68161737 MsaT014136.1:CDS
CCAGTATTAGACCCAGGCCC+AGG 0.531565 3:-68161896 None:intergenic
AATAATAGTTCTTATGAGAG+TGG 0.535058 3:+68161773 MsaT014136.1:CDS
GCGGACTCCGACAACGACTC+CGG 0.535824 3:+68161365 MsaT014136.1:CDS
ACACTTATCGGAGGCTTCGC+CGG 0.538515 3:-68161573 None:intergenic
TCCGGCGGACCACACGGAGG+AGG 0.541140 3:+68161383 MsaT014136.1:CDS
AACGACTCCGGCGGACCACA+CGG 0.545064 3:+68161377 MsaT014136.1:CDS
GACCCAGGCCCAGGATACCC+AGG 0.548272 3:-68161887 None:intergenic
TGTATGGTTCTGGTGGTGGT+GGG 0.552197 3:+68161837 MsaT014136.1:CDS
GAGTTCATCAGCTTCATCAC+CGG 0.553474 3:+68161554 MsaT014136.1:CDS
GCTTTGGGCGATGACGACGT+TGG 0.557062 3:+68161634 MsaT014136.1:CDS
GAGAAGCGGAAGACGATCAA+CGG 0.557275 3:+68161602 MsaT014136.1:CDS
CAGAGGTTTAGGGAGATGGA+AGG 0.558997 3:+68161695 MsaT014136.1:CDS
GAGTATGTTGAGCCGTTGAA+AGG 0.561542 3:+68161665 MsaT014136.1:CDS
CATCAAGTTATGGGTAAGGG+TGG 0.563450 3:+68161863 MsaT014136.1:CDS
ATAAGTGTCAGCGAGAGAAG+CGG 0.570892 3:+68161588 MsaT014136.1:CDS
TTTCATCAAGTTATGGGTAA+GGG 0.591667 3:+68161860 MsaT014136.1:CDS
ATCAAGTTATGGGTAAGGGT+GGG 0.596245 3:+68161864 MsaT014136.1:CDS
GGCCTGGGTCTAATACTGGT+AGG 0.607014 3:+68161900 MsaT014136.1:CDS
CTCTGAAGATAACCTTTCAA+CGG 0.607767 3:-68161677 None:intergenic
AGAAATCGGTCTTGTTCTCG+TGG 0.610491 3:-68161434 None:intergenic
AATAGTTCTTATGAGAGTGG+TGG 0.624003 3:+68161776 MsaT014136.1:CDS
ATGGAAGGAGAGAAGACGGT+GGG 0.627652 3:+68161710 MsaT014136.1:CDS
GATGGAAGGAGAGAAGACGG+TGG 0.628393 3:+68161709 MsaT014136.1:CDS
TTTGGGCGATGACGACGTTG+GGG 0.632604 3:+68161636 MsaT014136.1:CDS
GACTCCGACAACGACTCCGG+CGG 0.639609 3:+68161368 MsaT014136.1:CDS
CTCTCGCTGACACTTATCGG+AGG 0.641421 3:-68161582 None:intergenic
TTAACACTATTAGAACCTTG+CGG 0.662763 3:-68161752 None:intergenic
GGACACGTGTATGGTTCTGG+TGG 0.663222 3:+68161830 MsaT014136.1:CDS
GGCCTACCAGTATTAGACCC+AGG 0.676497 3:-68161902 None:intergenic
GGAGGAGGATCAAACGCACA+CGG 0.685824 3:+68161398 MsaT014136.1:CDS
GGTGGAGTTATGATGCATCA+GGG 0.718962 3:+68161809 MsaT014136.1:CDS
GACTCCGGCGGACCACACGG+AGG 0.719515 3:+68161380 MsaT014136.1:CDS
GCGTTTGATCCTCCTCCGTG+TGG 0.723554 3:-68161392 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 68161362 68161931 68161362 ID=MsaG014136
Chr3 mRNA 68161362 68161931 68161362 ID=MsaT014136.1;Parent=MsaG014136
Chr3 exon 68161362 68161931 68161362 ID=MsaT014136.1.exon1;Parent=MsaT014136.1
Chr3 CDS 68161362 68161931 68161362 ID=cds.MsaT014136.1;Parent=MsaT014136.1
Gene Sequence

>MsaT014136.1

ATGGCGGACTCCGACAACGACTCCGGCGGACCACACGGAGGAGGATCAAACGCACACGGAAGCGAGATGTCACCACGAGAACAAGACCGATTTCTACCAATAGCAAACGTAAGCAGGATCATGAAAAAAGCACTACCAGCGAACGCGAAGATCTCAAAAGACGCAAAAGAAACAGTTCAAGAGTGCGTTTCAGAGTTCATCAGCTTCATCACCGGCGAAGCCTCCGATAAGTGTCAGCGAGAGAAGCGGAAGACGATCAACGGCGATGATTTGCTTTGGGCGATGACGACGTTGGGGTTCGAGGAGTATGTTGAGCCGTTGAAAGGTTATCTTCAGAGGTTTAGGGAGATGGAAGGAGAGAAGACGGTGGGTGCGCGTGATAAAGACGCGCCGCAAGGTTCTAATAGTGTTAATAATAGTTCTTATGAGAGTGGTGGTTATGGTGGTGGTGGAGTTATGATGCATCAGGGACACGTGTATGGTTCTGGTGGTGGTGGGTTTCATCAAGTTATGGGTAAGGGTGGGCCTGGGTATCCTGGGCCTGGGTCTAATACTGGTAGGCCCAGATAG
Protein sequence

>MsaT014136.1

MADSDNDSGGPHGGGSNAHGSEMSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKGYLQRFREMEGEKTVGARDKDAPQGSNSVNNSSYESGGYGGGGVMMHQGHVYGSGGGGFHQVMGKGGPGYPGPGSNTGRPR*