AlfalfaGEDB | MsaG014151

M. sativa ssp. caerulea / MsaG014151

KEGG: K03321 K08065
GO: -
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014151	XP_004516113.2	44.958	238	119	2	1	238	6	231	2.73e-56	189

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014151	sp\|Q9SLG0\|NFYB1_ARATH	37.864	103	63	1	38	139	7	109	3.01e-21	89.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014151	tr\|A0A1S2Z6W5\|A0A1S2Z6W5_CICAR	44.958	238	119	2	1	238	6	231	1.30e-56	189

TFs/TRs:

Gene ID	Type	Classification
MsaG014151	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000380	MsaG014151	0.800037	2.905530e-48	3.931735e-46
MsaG001705	MsaG014151	0.813764	3.742144e-51	7.012748e-49
MsaG003067	MsaG014151	0.808620	4.825705e-50	7.971141e-48
MsaG006387	MsaG014151	0.809059	3.890660e-50	6.495160e-48
MsaG006725	MsaG014151	0.815972	1.219019e-51	2.415224e-49
MsaG007038	MsaG014151	0.812264	7.952750e-51	1.435499e-48
MsaG008631	MsaG014151	0.815159	1.845239e-51	3.581296e-49
MsaG011044	MsaG014151	0.803117	6.834297e-49	9.919103e-47
MsaG011256	MsaG014151	0.808216	5.878569e-50	9.616366e-48
MsaG042072	MsaG014151	0.824087	1.726884e-53	4.237170e-51
MsaG045263	MsaG014151	0.823804	2.010316e-53	4.895096e-51
MsaG045461	MsaG014151	0.821154	8.244688e-53	1.869608e-50
MsaG045590	MsaG014151	0.801406	1.531536e-48	2.137564e-46
MsaG000003	MsaG014151	0.806320	1.476077e-49	2.308194e-47
MsaG002307	MsaG014151	0.804465	3.597634e-49	5.386950e-47
MsaG014151	MsaG014799	0.810419	1.990809e-50	3.434793e-48
MsaG014151	MsaG013071	0.824973	1.071036e-53	2.692204e-51
MsaG015318	MsaG014151	0.830861	4.166975e-55	1.236081e-52
MsaG015372	MsaG014151	0.805010	2.772851e-49	4.204806e-47
MsaG019556	MsaG014151	0.834474	5.340199e-56	1.760912e-53
MsaG022737	MsaG014151	0.824278	1.558714e-53	3.844376e-51
MsaG023614	MsaG014151	0.813901	3.491456e-51	6.565626e-49
MsaG024620	MsaG014151	0.806008	1.716052e-49	2.663498e-47
MsaG025714	MsaG014151	0.832581	1.576893e-55	4.917059e-53
MsaG029105	MsaG014151	0.803941	4.620153e-49	6.834535e-47
MsaG032404	MsaG014151	0.819406	2.065251e-52	4.471600e-50
MsaG035255	MsaG014151	0.825456	8.241897e-54	2.099433e-51
MsaG035296	MsaG014151	0.810484	1.927654e-50	3.331042e-48
MsaG035634	MsaG014151	0.812866	5.880837e-51	1.077556e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014151	MtrunA17_Chr5g0446491	44.681	94	51	1	47	139	18	111	2.86e-22	90.1
MsaG014151	MtrunA17_Chr3g0102351	45.000	100	52	1	56	155	32	128	7.21e-22	89.4
MsaG014151	MtrunA17_Chr1g0191981	42.529	87	50	0	55	141	8	94	1.87e-21	86.7
MsaG014151	MtrunA17_Chr2g0290491	41.000	100	58	1	41	139	6	105	2.53e-21	87.4
MsaG014151	MtrunA17_Chr7g0263601	41.053	95	55	1	46	139	20	114	3.23e-21	87.0
MsaG014151	MtrunA17_Chr8g0384451	46.429	84	45	0	56	139	45	128	1.11e-20	86.7
MsaG014151	MtrunA17_Chr1g0185911	38.710	93	57	0	56	148	34	126	1.22e-20	85.5
MsaG014151	MtrunA17_Chr1g0165041	35.433	127	79	3	49	172	3	129	2.17e-20	85.5
MsaG014151	MtrunA17_Chr8g0382931	45.238	84	46	0	56	139	33	116	2.66e-20	85.5
MsaG014151	MtrunA17_Chr1g0195851	42.857	84	48	0	56	139	41	124	2.82e-20	85.1
MsaG014151	MtrunA17_Chr4g0076381	45.238	84	46	0	56	139	63	146	3.73e-20	85.9
MsaG014151	MtrunA17_Chr4g0076321	42.857	84	48	0	56	139	24	107	1.12e-18	79.3
MsaG014151	MtrunA17_Chr4g0067091	34.906	106	65	1	34	139	9	110	6.20e-18	79.3
MsaG014151	MtrunA17_Chr2g0296321	40.476	84	50	0	56	139	71	154	1.43e-17	79.3
MsaG014151	MtrunA17_Chr5g0446601	34.066	91	60	0	55	145	28	118	2.25e-16	74.7
MsaG014151	MtrunA17_Chr1g0159291	35.802	81	52	0	56	136	7	87	2.59e-12	61.2
MsaG014151	MtrunA17_Chr1g0159271	34.667	75	49	0	65	139	1	75	2.47e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014151	AT2G38880.7	36.607	112	70	1	38	148	7	118	9.96e-23	90.5
MsaG014151	AT2G38880.2	37.864	103	63	1	38	139	7	109	3.06e-22	89.4
MsaG014151	AT2G38880.1	37.864	103	63	1	38	139	7	109	3.06e-22	89.4
MsaG014151	AT2G38880.5	37.864	103	63	1	38	139	7	109	3.06e-22	89.4
MsaG014151	AT2G38880.3	37.864	103	63	1	38	139	7	109	3.85e-22	89.0
MsaG014151	AT2G38880.11	37.864	103	63	1	38	139	7	109	6.36e-22	89.0
MsaG014151	AT2G38880.8	37.864	103	63	1	38	139	7	109	6.36e-22	89.0
MsaG014151	AT4G14540.1	46.429	84	45	0	56	139	26	109	8.02e-22	89.0
MsaG014151	AT2G38880.4	37.864	103	63	1	38	139	7	109	1.05e-21	87.0
MsaG014151	AT2G37060.2	41.935	93	53	1	48	139	26	118	7.05e-21	86.7
MsaG014151	AT2G37060.3	41.935	93	53	1	48	139	26	118	7.05e-21	86.7
MsaG014151	AT2G37060.1	41.935	93	53	1	48	139	26	118	7.05e-21	86.7
MsaG014151	AT5G47640.1	46.429	84	45	0	56	139	32	115	1.03e-20	86.7
MsaG014151	AT1G21970.1	36.957	138	81	2	46	177	54	191	1.64e-20	87.4
MsaG014151	AT2G38880.9	37.864	103	63	1	38	139	7	109	1.90e-20	85.1
MsaG014151	AT2G38880.10	37.864	103	63	1	38	139	7	109	1.90e-20	85.1
MsaG014151	AT2G38880.6	37.864	103	63	1	38	139	7	109	1.90e-20	85.1
MsaG014151	AT2G13570.1	46.429	84	45	0	56	139	41	124	6.09e-20	85.1
MsaG014151	AT1G09030.1	40.476	84	50	0	56	139	8	91	2.10e-19	81.6
MsaG014151	AT3G53340.5	39.785	93	55	1	48	139	25	117	5.52e-19	81.6
MsaG014151	AT3G53340.1	39.785	93	55	1	48	139	25	117	5.52e-19	81.6
MsaG014151	AT2G47810.1	40.476	84	50	0	56	139	56	139	8.37e-19	80.9
MsaG014151	AT3G53340.4	39.785	93	55	1	48	139	25	117	1.24e-18	79.3
MsaG014151	AT5G47670.2	38.298	94	57	1	47	139	24	117	2.19e-18	80.9
MsaG014151	AT5G47670.1	38.298	94	57	1	47	139	53	146	3.08e-18	80.9
MsaG014151	AT5G47670.3	38.298	94	57	1	47	139	53	146	3.08e-18	80.9
MsaG014151	AT3G53340.3	39.785	93	55	1	48	139	25	117	5.83e-18	78.2
MsaG014151	AT3G53340.2	39.785	93	55	1	48	139	25	117	5.83e-18	78.2

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGAATGAATTGGATAATT+TGG	0.149282	3:+68270932	MsaT014151.1:CDS
CAGGGAATGCAGGAAAATTT+TGG	0.155460	3:+68270644	MsaT014151.1:CDS
CTCAAACTCATTTATTGATT+TGG	0.201982	3:+68270884	MsaT014151.1:CDS
ATTAAGGGTCATAAAGTCTA+TGG	0.242488	3:+68270680	MsaT014151.1:CDS
TTTCGTTATAGTAATGAAAT+TGG	0.264459	3:+68270747	MsaT014151.1:CDS
CCACCGCCACCCATTGCAAT+TGG	0.296308	3:+68270843	MsaT014151.1:CDS
CTTGGATTCATTATACTTGT+TGG	0.316399	3:-68270432	None:intergenic
GGCACAAGGGCATCATTTCT+TGG	0.320738	3:-68270450	None:intergenic
GGGTGGCGGTGGTGGTAAGG+TGG	0.341905	3:-68270832	None:intergenic
TAGACTTTATGACCCTTAAT+AGG	0.346002	3:-68270677	None:intergenic
TGGAATGAATTGGATAATTT+GGG	0.348468	3:+68270933	MsaT014151.1:CDS
GATGATAAAATGAATGATTA+AGG	0.370091	3:+68271035	MsaT014151.1:CDS
TTTGTCTCGAATTGCTAGTT+GGG	0.382494	3:-68270472	None:intergenic
GAATGAGGTTTGGAATGAAT+TGG	0.382994	3:+68270923	MsaT014151.1:CDS
TGGGTGCAGATGACCTATTA+AGG	0.383571	3:+68270664	MsaT014151.1:CDS
AAGTTTGTTAAAGCAGTCTC+TGG	0.385163	3:-68270966	None:intergenic
CCACCCATTGCAATTGGGTC+AGG	0.408457	3:+68270849	MsaT014151.1:CDS
TACATATCTTATAAACTTTG+AGG	0.420260	3:-68270595	None:intergenic
GGGTGCAGATGACCTATTAA+GGG	0.425849	3:+68270665	MsaT014151.1:CDS
TTCCCTGTGACAACGTTCAA+TGG	0.434461	3:-68270628	None:intergenic
ATTGGATATTTGAAACTTGT+TGG	0.437848	3:+68270765	MsaT014151.1:CDS
CAGCGGGCATGAATGAGGTT+TGG	0.446236	3:+68270913	MsaT014151.1:CDS
GGTGGCGGTGGTGGTAAGGT+GGG	0.449680	3:-68270831	None:intergenic
CACCGCCACCCATTGCAATT+GGG	0.468956	3:+68270844	MsaT014151.1:CDS
CCAATTGCAATGGGTGGCGG+TGG	0.473098	3:-68270843	None:intergenic
AATGGGTGGCGGTGGTGGTA+AGG	0.484109	3:-68270835	None:intergenic
ATTGCAATGGGTGGCGGTGG+TGG	0.491572	3:-68270840	None:intergenic
GAATTGGATAATTTGGGTGC+TGG	0.493441	3:+68270939	MsaT014151.1:CDS
AAGCCATTGAACGTTGTCAC+AGG	0.498720	3:+68270625	MsaT014151.1:CDS
TGACGATATCTTTCAAGAGA+TGG	0.505210	3:+68271013	MsaT014151.1:CDS
AACGTTGTCACAGGGAATGC+AGG	0.505564	3:+68270634	MsaT014151.1:CDS
GACCCAATTGCAATGGGTGG+CGG	0.506575	3:-68270846	None:intergenic
AGTTTGTTAAAGCAGTCTCT+GGG	0.515893	3:-68270965	None:intergenic
TCAAAATCCAACTCAAGCTC+TGG	0.519412	3:+68270128	MsaT014151.1:CDS
ATCTGAAAAGCTGGTAAGGT+GGG	0.524201	3:-68270801	None:intergenic
CTCTCGTGGAGCAAAGGAGA+TGG	0.534626	3:+68270560	MsaT014151.1:CDS
AAGCCTGACCCAATTGCAAT+GGG	0.536639	3:-68270852	None:intergenic
AAAGCTGGTAAGGTGGGCTT+AGG	0.541538	3:-68270795	None:intergenic
TGAAGCAGCGGGCATGAATG+AGG	0.557960	3:+68270908	MsaT014151.1:CDS
GAATTGCTAGTTGGGGCACA+AGG	0.564590	3:-68270464	None:intergenic
ATTGATTTGGATGAAGCAGC+GGG	0.566122	3:+68270897	MsaT014151.1:CDS
TTGTCTCGAATTGCTAGTTG+GGG	0.571725	3:-68270471	None:intergenic
TAAGCCTGACCCAATTGCAA+TGG	0.575839	3:-68270853	None:intergenic
TTAAGGGTCATAAAGTCTAT+GGG	0.580731	3:+68270681	MsaT014151.1:CDS
AGCGATCTGAAAAGCTGGTA+AGG	0.583971	3:-68270805	None:intergenic
GGAGAAGCGATCTGAAAAGC+TGG	0.592152	3:-68270810	None:intergenic
AGCCATTGAACGTTGTCACA+GGG	0.613629	3:+68270626	MsaT014151.1:CDS
AAATATTCTCTCGTTAACAA+TGG	0.617869	3:+68270105	MsaT014151.1:CDS
GATCTGAAAAGCTGGTAAGG+TGG	0.629020	3:-68270802	None:intergenic
TATTGATTTGGATGAAGCAG+CGG	0.641197	3:+68270896	MsaT014151.1:CDS
CCTGACCCAATTGCAATGGG+TGG	0.641990	3:-68270849	None:intergenic
AATTGCTAGTTGGGGCACAA+GGG	0.680281	3:-68270463	None:intergenic
GTTTGTTAAAGCAGTCTCTG+GGG	0.684155	3:-68270964	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	68270096	68271055	68270096	ID=MsaG014151
Chr3	mRNA	68270096	68271055	68270096	ID=MsaT014151.1;Parent=MsaG014151
Chr3	exon	68270096	68270180	68270096	ID=MsaT014151.1.exon1;Parent=MsaT014151.1
Chr3	CDS	68270096	68270180	68270096	ID=cds.MsaT014151.1;Parent=MsaT014151.1
Chr3	exon	68270421	68271055	68270421	ID=MsaT014151.1.exon2;Parent=MsaT014151.1
Chr3	CDS	68270421	68271055	68270421	ID=cds.MsaT014151.1;Parent=MsaT014151.1

Gene Sequence

>MsaT014151.1

ATGGAGAATAAATATTCTCTCGTTAACAATGGTCAAAATCCAACTCAAGCTCTGGTAAAACACAAAATCAGAATAACTTACACACATACAAGAGCTCCAACAAGTATAATGAATCCAAGAAATGATGCCCTTGTGCCCCAACTAGCAATTCGAGACAAAAATACCTTGCCAATAAAAAATGTGGCAACAATCATGAGAAAAGTTCTTCCACCAAACACAAAAATCTCTCGTGGAGCAAAGGAGATGGTTGAACAAATCACCTCAAAGTTTATAAGATATGTAACCAAAAAAGCCATTGAACGTTGTCACAGGGAATGCAGGAAAATTTTGGGTGCAGATGACCTATTAAGGGTCATAAAGTCTATGGGTTATAATAATTATTATGAGTATCTCAATTTTTATCTCAAATGCTTTCGTTATAGTAATGAAATTGGATATTTGAAACTTGTTGGTGAGCTTCCTAAGCCCACCTTACCAGCTTTTCAGATCGCTTCTCCCACCTTACCACCACCGCCACCCATTGCAATTGGGTCAGGCTTATCTTTGAACTCAAACTCATTTATTGATTTGGATGAAGCAGCGGGCATGAATGAGGTTTGGAATGAATTGGATAATTTGGGTGCTGGTTCCCCAGAGACTGCTTTAACAAACTTTGATCATTCAATTCAATTCAACTTTGACGATATCTTTCAAGAGATGGATGATAAAATGAATGATTAA

Protein sequence

>MsaT014151.1

MENKYSLVNNGQNPTQALVKHKIRITYTHTRAPTSIMNPRNDALVPQLAIRDKNTLPIKNVATIMRKVLPPNTKISRGAKEMVEQITSKFIRYVTKKAIERCHRECRKILGADDLLRVIKSMGYNNYYEYLNFYLKCFRYSNEIGYLKLVGELPKPTLPAFQIASPTLPPPPPIAIGSGLSLNSNSFIDLDEAAGMNEVWNELDNLGAGSPETALTNFDHSIQFNFDDIFQEMDDKMND*