AlfalfaGEDB | MsaG014158

M. sativa ssp. caerulea / MsaG014158

KEGG: -
GO: GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014158	XP_027354476.1	60.150	133	31	1	28	138	188	320	3.57e-41	152

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014158	sp\|Q5Z4U5\|GAT20_ORYSJ	40.659	91	39	2	26	114	251	328	4.99e-12	67.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014158	tr\|A0A1S2Y9V5\|A0A1S2Y9V5_CICAR	57.037	135	36	1	26	138	189	323	7.05e-39	146

TFs/TRs:

Gene ID	Type	Classification
MsaG014158	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014158	MtrunA17_Chr3g0132751	54.815	135	39	1	26	138	196	330	3.60e-37	132
MsaG014158	MtrunA17_Chr3g0102511	34.946	186	88	4	11	164	10	194	7.30e-21	89.7
MsaG014158	MtrunA17_Chr4g0049741	38.596	114	54	4	30	128	175	287	6.78e-15	72.0
MsaG014158	MtrunA17_Chr3g0132761	52.542	59	26	1	32	90	200	256	8.21e-13	65.9
MsaG014158	MtrunA17_Chr5g0427661	39.759	83	43	2	32	111	203	281	3.56e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014158	AT1G51600.1	62.791	43	16	0	32	74	223	265	7.70e-13	66.2
MsaG014158	AT1G51600.2	62.791	43	16	0	32	74	223	265	7.70e-13	66.2
MsaG014158	AT3G21175.2	62.791	43	16	0	32	74	217	259	6.47e-12	63.5
MsaG014158	AT3G21175.1	62.791	43	16	0	32	74	219	261	6.61e-12	63.5
MsaG014158	AT4G24470.1	43.056	72	36	1	32	103	214	280	7.87e-12	63.5
MsaG014158	AT4G24470.2	43.056	72	36	1	32	103	214	280	7.87e-12	63.5
MsaG014158	AT4G24470.3	56.818	44	19	0	32	75	214	257	2.07e-11	62.4

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCGTCACGTGGGCCAAAA+AGG	0.305959	3:+68387189	MsaT014158.1:CDS
AAGCGTTTAAACTGCCTATC+AGG	0.314872	3:-68386960	None:intergenic
GTTGGGTGTTCTGAAGATTC+AGG	0.326610	3:-68389062	None:intergenic
GTTGGGTGTTCTGAAGATTC+AGG	0.326610	3:-68391180	None:intergenic
TTTAAAACTACCTCTTCAAC+GGG	0.359063	3:-68388916	None:intergenic
ACCTTCAGTAGTTGTGGCCT+TGG	0.379149	3:-68390999	None:intergenic
CAAATGCGTCCCGGGCCAGC+TGG	0.413545	3:+68387142	MsaT014158.1:CDS
CCAACAAACACTCTACCATT+AGG	0.432811	3:+68389080	MsaT014158.1:CDS
AGTAAATCTTCCAGCTGGCC+CGG	0.440593	3:-68387152	None:intergenic
TGTGGCCTTGGAAGGATCTT+CGG	0.456624	3:-68390987	None:intergenic
TTCTGCTGATCAAAGTGGTC+TGG	0.457476	3:+68387723	MsaT014158.1:CDS
TCCAAGCTCATTGTTGCCGA+TGG	0.464599	3:+68388871	MsaT014158.1:CDS
GAAGAATGAACAATTCCTAA+TGG	0.472907	3:-68389095	None:intergenic
CATTCTTCAGAAAATGATGA+TGG	0.478804	3:+68389110	MsaT014158.1:CDS
CTAACGGTAGAGTGTTTGTT+GGG	0.480089	3:-68391197	None:intergenic
CCTAACGGTAGAGTGTTTGT+TGG	0.483122	3:-68391198	None:intergenic
AAACGCTTCAAGAAAAGTCC+TGG	0.483720	3:+68386975	MsaT014158.1:CDS
CTAATGGTAGAGTGTTTGTT+GGG	0.483860	3:-68389079	None:intergenic
AGGATGCTGCGTCACAAGGA+TGG	0.484263	3:+68386915	None:intergenic
TGCATTGAACCCTATTGAAA+AGG	0.485054	3:+68391033	MsaT014158.1:CDS
CCTAATGGTAGAGTGTTTGT+TGG	0.500474	3:-68389080	None:intergenic
TTCAGAAAATGATGATGGAC+AGG	0.501286	3:+68389115	MsaT014158.1:CDS
TCAGTAGTTGTGGCCTTGGA+AGG	0.504019	3:-68390995	None:intergenic
ATTTAAAACTACCTCTTCAA+CGG	0.506639	3:-68388917	None:intergenic
ACCATCGGCAACAATGAGCT+TGG	0.511631	3:-68388872	None:intergenic
AACACTTCTCAAATGCGTCC+CGG	0.512353	3:+68387133	MsaT014158.1:CDS
TGCATGATTGTTCGAACCAT+CGG	0.515819	3:-68388887	None:intergenic
GCTTCAAGCGAAGTCCTGAT+AGG	0.522695	3:+68386946	MsaT014158.1:CDS
TCGGCAACAATGAGCTTGGA+AGG	0.523449	3:-68388868	None:intergenic
ACACTTCTCAAATGCGTCCC+GGG	0.528359	3:+68387134	MsaT014158.1:CDS
TTCAGAAAATGATAATGTCC+AGG	0.532128	3:+68391233	MsaT014158.1:CDS
TGCTAGGATGCTGCGTCACA+AGG	0.532793	3:+68386911	None:intergenic
GGATGCTGCGTCACAAGGAT+GGG	0.534910	3:+68386916	None:intergenic
TGCATTGAACCCCGTTGAAG+AGG	0.538463	3:+68388906	MsaT014158.1:CDS
GTAAATCTTCCAGCTGGCCC+GGG	0.542997	3:-68387151	None:intergenic
CCAACAAACACTCTACCGTT+AGG	0.550586	3:+68391198	MsaT014158.1:CDS
ATTCGAACCTTCAGTAGTTG+TGG	0.584866	3:-68391005	None:intergenic
CATAGAGTAAATCTTCCAGC+TGG	0.585448	3:-68387157	None:intergenic
TCCAAGGCCACAACTACTGA+AGG	0.589066	3:+68390998	MsaT014158.1:CDS
ACTCATTCTGCTGATCAAAG+TGG	0.600709	3:+68387718	MsaT014158.1:CDS
TATTTCCGAAGATCCTTCCA+AGG	0.605617	3:+68390982	MsaT014158.1:CDS
GTAATACATGCGGCGTCACG+TGG	0.612083	3:+68387179	MsaT014158.1:CDS
TGCTGCGTCACAAGGATGGG+CGG	0.620721	3:+68386919	MsaT014158.1:CDS
GAAGAATGATCAATTCCTAA+CGG	0.628796	3:-68391213	None:intergenic
AGACGTAGATGTCAACATTG+TGG	0.642693	3:+68387097	MsaT014158.1:intron
CGGCAACAATGAGCTTGGAA+GGG	0.643164	3:-68388867	None:intergenic
TTTAAAACTCACAAAGAACC+AGG	0.655376	3:-68386993	None:intergenic
TTTACTCTATGTAATACATG+CGG	0.695440	3:+68387169	MsaT014158.1:CDS
TAATACATGCGGCGTCACGT+GGG	0.699536	3:+68387180	MsaT014158.1:CDS
TTAAAACTACCTCTTCAACG+GGG	0.758088	3:-68388915	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	68386918	68392274	68386918	ID=MsaG014158
Chr3	mRNA	68386918	68392274	68386918	ID=MsaT014158.1;Parent=MsaG014158
Chr3	exon	68386918	68387003	68386918	ID=MsaT014158.1.exon1;Parent=MsaT014158.1
Chr3	CDS	68386918	68387003	68386918	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68387099	68387210	68387099	ID=MsaT014158.1.exon2;Parent=MsaT014158.1
Chr3	CDS	68387099	68387210	68387099	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68387685	68387744	68387685	ID=MsaT014158.1.exon3;Parent=MsaT014158.1
Chr3	CDS	68387685	68387744	68387685	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68388853	68388927	68388853	ID=MsaT014158.1.exon4;Parent=MsaT014158.1
Chr3	CDS	68388853	68388927	68388853	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68389056	68389136	68389056	ID=MsaT014158.1.exon5;Parent=MsaT014158.1
Chr3	CDS	68389056	68389136	68389056	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68390980	68391054	68390980	ID=MsaT014158.1.exon6;Parent=MsaT014158.1
Chr3	CDS	68390980	68391054	68390980	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68391174	68391254	68391174	ID=MsaT014158.1.exon7;Parent=MsaT014158.1
Chr3	CDS	68391174	68391254	68391174	ID=cds.MsaT014158.1;Parent=MsaT014158.1
Chr3	exon	68392233	68392274	68392233	ID=MsaT014158.1.exon8;Parent=MsaT014158.1
Chr3	CDS	68392233	68392274	68392233	ID=cds.MsaT014158.1;Parent=MsaT014158.1

Gene Sequence

>MsaT014158.1

ATGCTGCGTCACAAGGATGGGCGGTTACGCTTCAAGCGAAGTCCTGATAGGCAGTTTAAACGCTTCAAGAAAAGTCCTGGTTCTTTACGTAGATGTCAACATTGTGGACTCGACGAAAATAACACTTCTCAAATGCGTCCCGGGCCAGCTGGAAGATTTACTCTATGTAATACATGCGGCGTCACGTGGGCCAAAAAGGGGATACTTAGAGATCTCAGTAAGAGACGAAGAACTCATTCTGCTGATCAAAGTGGTCTGGGTATTTCTGAAGACCCTTCCAAGCTCATTGTTGCCGATGGTTCGAACAATCATGCATTGAACCCCGTTGAAGAGGAATTGCCTGAATCTTCAGAACACCCAACAAACACTCTACCATTAGGAATTGTTCATTCTTCAGAAAATGATGATGGACAGAGTATTTCCGAAGATCCTTCCAAGGCCACAACTACTGAAGGTTCGAATTATCATGCATTGAACCCTATTGAAAAGGAATTGCCTGAATCTTCAGAACACCCAACAAACACTCTACCGTTAGGAATTGATCATTCTTCAGAAAATGATAATGTCCAGGCCGTGGGGGTGCATAAGGTGGGAGGTCAAAATATTTCATGA

Protein sequence

>MsaT014158.1

MLRHKDGRLRFKRSPDRQFKRFKKSPGSLRRCQHCGLDENNTSQMRPGPAGRFTLCNTCGVTWAKKGILRDLSKRRRTHSADQSGLGISEDPSKLIVADGSNNHALNPVEEELPESSEHPTNTLPLGIVHSSENDDGQSISEDPSKATTTEGSNYHALNPIEKELPESSEHPTNTLPLGIDHSSENDNVQAVGVHKVGGQNIS*