AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014229


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014229 MtrunA17_Chr3g0103031 100.000 330 0 0 33 362 1 330 0.0 681
MsaG014229 MtrunA17_Chr5g0447111 63.580 324 115 3 38 360 8 329 2.05e-146 417
MsaG014229 MtrunA17_Chr1g0159831 50.450 333 157 4 33 360 1 330 1.02e-116 341
MsaG014229 MtrunA17_Chr3g0124921 51.875 320 150 4 39 356 4 321 2.84e-116 340
MsaG014229 MtrunA17_Chr5g0447191 56.111 180 77 1 179 356 8 187 3.39e-58 187
MsaG014229 MtrunA17_Chr4g0029841 34.031 191 122 1 164 354 90 276 9.87e-30 115
MsaG014229 MtrunA17_Chr7g0259501 27.815 151 94 3 187 324 197 345 1.74e-11 65.1
MsaG014229 MtrunA17_Chr1g0185861 28.289 152 94 3 187 325 201 350 2.08e-11 64.7
MsaG014229 MtrunA17_Chr3g0114411 30.108 186 122 4 166 343 66 251 2.39e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014229 AT1G55760.1 70.091 331 96 3 33 362 1 329 2.41e-173 485
MsaG014229 AT1G21780.2 51.553 322 154 2 39 360 5 324 2.45e-121 353
MsaG014229 AT1G21780.1 51.553 322 154 2 39 360 5 324 2.45e-121 353
MsaG014229 AT1G01640.1 30.366 191 128 4 174 361 1 189 1.40e-15 75.1
MsaG014229 AT1G01640.2 30.588 170 113 4 195 361 1 168 4.86e-13 67.4
MsaG014229 AT3G03740.1 31.959 194 114 5 181 358 199 390 1.62e-12 68.9
MsaG014229 AT3G56230.1 29.651 172 116 4 184 351 98 268 3.08e-12 67.0
MsaG014229 AT2G40450.1 32.540 126 74 4 178 292 4 129 1.12e-11 63.9

Find 76 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTGAGGTTCCACTTTC+TGG 0.064092 3:+69421986 MsaT014229.1:CDS
CTTGTAACATTGGATATTTC+AGG 0.153912 3:-69421500 None:intergenic
ATCCAGTATCTTGTTAAATT+TGG 0.170654 3:+69423276 MsaT014229.1:CDS
CGACATGTCTGTGATGTTTA+TGG 0.202175 3:-69423028 None:intergenic
TTGGTAAAATATTTGAAATT+CGG 0.228336 3:+69423295 MsaT014229.1:CDS
TGAGATCCCTATCCGCATTT+TGG 0.242657 3:-69423338 None:intergenic
TGGTAAAATATTTGAAATTC+GGG 0.243014 3:+69423296 MsaT014229.1:CDS
ATCTCATCGCTGAAGTCTTT+GGG 0.249673 3:+69423355 MsaT014229.1:CDS
ACCGATATAGTGATGCATTT+TGG 0.251091 3:-69423230 None:intergenic
GAAGTCTTTGGGGAAGTTCT+TGG 0.305595 3:+69423366 MsaT014229.1:CDS
GATCTCATCGCTGAAGTCTT+TGG 0.318696 3:+69423354 MsaT014229.1:CDS
TTCCTTGATTTGAAGATTGC+AGG 0.320978 3:+69422031 MsaT014229.1:CDS
ATCCTTTCAAGATTGGTATC+TGG 0.328253 3:+69421029 MsaT014229.1:CDS
TACCAAATTTAACAAGATAC+TGG 0.332501 3:-69423278 None:intergenic
TCAAGATTGGTATCTGGAAT+TGG 0.343346 3:+69421035 MsaT014229.1:CDS
TTGGGGAAGTTCTTGGTGCC+TGG 0.360323 3:+69423373 MsaT014229.1:CDS
TCCAAAATGCATCACTATAT+CGG 0.363703 3:+69423229 MsaT014229.1:CDS
CTCTGTGAACTGCCCTCAAA+TGG 0.371138 3:+69413599 MsaT014229.1:CDS
AAATCTGATCCTTTCAAGAT+TGG 0.383983 3:+69421022 MsaT014229.1:CDS
AGAACTTCAATATCCTTTCC+AGG 0.396258 3:-69423391 None:intergenic
GATCGTGTGAAAACATGCTT+TGG 0.410502 3:-69422984 None:intergenic
AAATCCTTCACCACCCCATA+TGG 0.411517 3:-69422818 None:intergenic
GTGGAGAACCTTGCTCCATA+TGG 0.412309 3:+69422803 MsaT014229.1:CDS
CAACTCCTCGTCTTGCTCAA+TGG 0.443191 3:+69420966 MsaT014229.1:CDS
AGGATTATCTCTTGTAACAT+TGG 0.466890 3:-69421510 None:intergenic
ACGCCGTCGTATCCCATTTG+AGG 0.471623 3:-69413612 None:intergenic
TGGAGAACCTTGCTCCATAT+GGG 0.474713 3:+69422804 MsaT014229.1:CDS
GCAAAAGCCCTAGGGTCTCT+TGG 0.476148 3:+69422859 MsaT014229.1:CDS
CGAAAAGCTCTCACACATCC+AGG 0.477842 3:+69421581 MsaT014229.1:CDS
CATGTCGATTGAAGTATGTC+AGG 0.482314 3:+69423044 MsaT014229.1:CDS
AATGCTTCTGATGGAAGTAT+AGG 0.485034 3:+69422922 MsaT014229.1:CDS
ATTCCTCCAAAATGCGGATA+GGG 0.487891 3:+69423332 MsaT014229.1:CDS
GCTTTGGAAGACAGGTGAAC+GGG 0.489275 3:-69422968 None:intergenic
CGCCGTCGTATCCCATTTGA+GGG 0.493282 3:-69413611 None:intergenic
TGCTCCATATGGGGTGGTGA+AGG 0.497886 3:+69422814 MsaT014229.1:CDS
GGCCTGCAATCTTCAAATCA+AGG 0.500364 3:-69422033 None:intergenic
AGAGGATGGAAGGTCGGAAT+GGG 0.505082 3:-69413580 None:intergenic
TATTCCTCCAAAATGCGGAT+AGG 0.515510 3:+69423331 MsaT014229.1:CDS
TGAGGGCAGTTCACAGAGGA+TGG 0.521332 3:-69413594 None:intergenic
CAGAGGATGGAAGGTCGGAA+TGG 0.521992 3:-69413581 None:intergenic
CTATCGTATTGCTTTGTCAA+AGG 0.529964 3:-69413641 None:intergenic
GCATTCTACCAAGAGACCCT+AGG 0.531172 3:-69422867 None:intergenic
ATCACAATCAATGCTTCTGA+TGG 0.531417 3:+69422913 MsaT014229.1:CDS
AGATCGAACGCAAAAGCCCT+AGG 0.533227 3:+69422850 MsaT014229.1:CDS
TCAATGGAGAATCGATAACT+TGG 0.536092 3:+69420982 MsaT014229.1:CDS
GAAGTTCTTGGTGCCTGGAA+AGG 0.537759 3:+69423378 MsaT014229.1:CDS
GAAAACATGCTTTGGAAGAC+AGG 0.538730 3:-69422976 None:intergenic
CGCATGATAAAAGAAGCAAT+TGG 0.545571 3:-69421533 None:intergenic
TCTGTGAACTGCCCTCAAAT+GGG 0.545784 3:+69413600 MsaT014229.1:CDS
CGTGTCGTTAGTTCTGCTCA+TGG 0.548473 3:+69421554 MsaT014229.1:CDS
ATGAAGAATTTCTGACACAT+AGG 0.551928 3:+69423100 MsaT014229.1:CDS
ATGATAAAAGAAGCAATTGG+AGG 0.567311 3:-69421530 None:intergenic
GGCAGTTCACAGAGGATGGA+AGG 0.573513 3:-69413590 None:intergenic
CATTCTACCAAGAGACCCTA+GGG 0.576970 3:-69422866 None:intergenic
GGAGAACCTTGCTCCATATG+GGG 0.579524 3:+69422805 MsaT014229.1:CDS
TATCTTCTAGAAGACTCTCA+TGG 0.579860 3:-69423179 None:intergenic
CAATTTATTCCTCCAAAATG+CGG 0.584842 3:+69423326 MsaT014229.1:CDS
TTCTAGAAGACTCTCATGGA+TGG 0.591651 3:-69423175 None:intergenic
TTCCAGATACCAATCTTGAA+AGG 0.598585 3:-69421031 None:intergenic
TCTCATCGCTGAAGTCTTTG+GGG 0.603546 3:+69423356 MsaT014229.1:CDS
GGGTGGTGAAGGATTTACTC+AGG 0.609959 3:+69422825 MsaT014229.1:CDS
TGTGAAATGAAGGTATTTGT+CGG 0.609961 3:+69421442 MsaT014229.1:intron
CTTGGTAGAATGCTAAGAGA+AGG 0.615271 3:+69422877 MsaT014229.1:CDS
GTTCACAGAGGATGGAAGGT+CGG 0.615999 3:-69413586 None:intergenic
TGCTTTGGAAGACAGGTGAA+CGG 0.624572 3:-69422969 None:intergenic
TGCCCTCAAATGGGATACGA+CGG 0.634372 3:+69413609 MsaT014229.1:CDS
TCACCACCCCATATGGAGCA+AGG 0.646739 3:-69422811 None:intergenic
GATCGAACGCAAAAGCCCTA+GGG 0.649072 3:+69422851 MsaT014229.1:CDS
TGACATATCTGACTTGAGAG+AGG 0.662279 3:+69423152 MsaT014229.1:CDS
TTTGTCGGTGGAGAAGCACA+AGG 0.689916 3:+69421457 MsaT014229.1:CDS
TCAAATGGGATACGACGGCG+TGG 0.691759 3:+69413614 MsaT014229.1:CDS
GAAATGAAGGTATTTGTCGG+TGG 0.696589 3:+69421445 MsaT014229.1:intron
ATTCTCCATTGAGCAAGACG+AGG 0.697408 3:-69420971 None:intergenic
GCTAGCGCTTCTGCATGCAG+CGG 0.709404 3:+69423122 MsaT014229.1:CDS
GAACCTTGCTCCATATGGGG+TGG 0.727934 3:+69422808 MsaT014229.1:CDS
CATTTGAGGGCAGTTCACAG+AGG 0.735622 3:-69413598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 69413572 69423407 69413572 ID=MsaG014229
Chr3 mRNA 69413572 69423407 69413572 ID=MsaT014229.1;Parent=MsaG014229
Chr3 exon 69413572 69413666 69413572 ID=MsaT014229.1.exon1;Parent=MsaT014229.1
Chr3 CDS 69413572 69413666 69413572 ID=cds.MsaT014229.1;Parent=MsaT014229.1
Chr3 exon 69420937 69421056 69420937 ID=MsaT014229.1.exon2;Parent=MsaT014229.1
Chr3 CDS 69420937 69421056 69420937 ID=cds.MsaT014229.1;Parent=MsaT014229.1
Chr3 exon 69421454 69421602 69421454 ID=MsaT014229.1.exon3;Parent=MsaT014229.1
Chr3 CDS 69421454 69421602 69421454 ID=cds.MsaT014229.1;Parent=MsaT014229.1
Chr3 exon 69421942 69422060 69421942 ID=MsaT014229.1.exon4;Parent=MsaT014229.1
Chr3 CDS 69421942 69422060 69421942 ID=cds.MsaT014229.1;Parent=MsaT014229.1
Chr3 exon 69422802 69423407 69422802 ID=MsaT014229.1.exon5;Parent=MsaT014229.1
Chr3 CDS 69422802 69423407 69422802 ID=cds.MsaT014229.1;Parent=MsaT014229.1
Gene Sequence

>MsaT014229.1

ATGCAATTCCCATTCCGACCTTCCATCCTCTGTGAACTGCCCTCAAATGGGATACGACGGCGTGGAGTTCCTTTGACAAAGCAATACGATAGCGCAATGACTGATTCTCTTTACAAAGTTGACACAACTCCTCGTCTTGCTCAATGGAGAATCGATAACTTGGCTTCTTGTACTTATCGCAAATCTGATCCTTTCAAGATTGGTATCTGGAATTGGTATTTGTCGGTGGAGAAGCACAAGGTTGTGTTTGTTAAATTGTTTCCTGAAATATCCAATGTTACAAGAGATAATCCTCCAATTGCTTCTTTTATCATGCGTGTCGTTAGTTCTGCTCATGGAAATCGAAAAGCTCTCACACATCCAGAGATAAAAGACAAAGTGATCAAGAGCAATGAAGATTTTGTTTGGTCAATTGAGGTTCCACTTTCTGGAAAATTCATCATTGATGTTGATTTCCTTGATTTGAAGATTGCAGGCCCAGATGGTGGAGAACCTTGCTCCATATGGGGTGGTGAAGGATTTACTCAGGAGAGATCGAACGCAAAAGCCCTAGGGTCTCTTGGTAGAATGCTAAGAGAAGGCATTCACACAGACATCACAATCAATGCTTCTGATGGAAGTATAGGAGTTCATAGAGCTCTTCTAGCTGCCCGTTCACCTGTCTTCCAAAGCATGTTTTCACACGATCTTAAAGAGAAAGAGTTGTCAACCATAAACATCACAGACATGTCGATTGAAGTATGTCAGGCTTTTCTCAATTATCTTTATGGGATCATCAAAAATGAAGAATTTCTGACACATAGGCTAGCGCTTCTGCATGCAGCGGACAAGTATGACATATCTGACTTGAGAGAGGCCATCCATGAGAGTCTTCTAGAAGATATTGATACGAAAAATGTACTTGAAAGGCTCCAAAATGCATCACTATATCGGTTGACGAAACTGAAGATGCATTGTATCCAGTATCTTGTTAAATTTGGTAAAATATTTGAAATTCGGGATGATTTCAATTTATTCCTCCAAAATGCGGATAGGGATCTCATCGCTGAAGTCTTTGGGGAAGTTCTTGGTGCCTGGAAAGGATATTGA

Protein sequence

>MsaT014229.1

MQFPFRPSILCELPSNGIRRRGVPLTKQYDSAMTDSLYKVDTTPRLAQWRIDNLASCTYRKSDPFKIGIWNWYLSVEKHKVVFVKLFPEISNVTRDNPPIASFIMRVVSSAHGNRKALTHPEIKDKVIKSNEDFVWSIEVPLSGKFIIDVDFLDLKIAGPDGGEPCSIWGGEGFTQERSNAKALGSLGRMLREGIHTDITINASDGSIGVHRALLAARSPVFQSMFSHDLKEKELSTINITDMSIEVCQAFLNYLYGIIKNEEFLTHRLALLHAADKYDISDLREAIHESLLEDIDTKNVLERLQNASLYRLTKLKMHCIQYLVKFGKIFEIRDDFNLFLQNADRDLIAEVFGEVLGAWKGY*