Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014246 | XP_003600453.1 | 82.042 | 284 | 50 | 1 | 1 | 283 | 1 | 284 | 5.74e-168 | 476 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014246 | sp|Q8L3W1|VRN1_ARATH | 27.059 | 340 | 179 | 9 | 8 | 283 | 1 | 335 | 3.25e-30 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014246 | tr|G8A2Z4|G8A2Z4_MEDTR | 82.042 | 284 | 50 | 1 | 1 | 283 | 1 | 284 | 2.74e-168 | 476 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG014246 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG004993 | MsaG014246 | 0.812709 | 6.363902e-51 | 1.161510e-48 |
| MsaG014154 | MsaG014246 | 0.957757 | 4.367986e-115 | 1.869621e-109 |
| MsaG014246 | MsaG014961 | 0.800245 | 2.637550e-48 | 3.585803e-46 |
| MsaG014246 | MsaG027329 | 0.826459 | 4.775499e-54 | 1.250899e-51 |
| MsaG014246 | MsaG033125 | 0.803636 | 5.343013e-49 | 7.847928e-47 |
| MsaG014246 | MsaG040209 | 0.801970 | 1.175203e-48 | 1.661351e-46 |
| MsaG014246 | MsaG045800 | 0.818567 | 3.197049e-52 | 6.771514e-50 |
| MsaG014246 | MsaG046616 | 0.800830 | 2.006901e-48 | 2.764736e-46 |
| MsaG014246 | MsaG019845 | 0.804948 | 2.856360e-49 | 4.325174e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014246 | MtrunA17_Chr3g0103191 | 79.681 | 251 | 50 | 1 | 1 | 250 | 1 | 251 | 2.63e-145 | 407 |
| MsaG014246 | MtrunA17_Chr3g0103181 | 73.592 | 284 | 69 | 4 | 1 | 283 | 1 | 279 | 1.33e-143 | 404 |
| MsaG014246 | MtrunA17_Chr3g0103171 | 70.992 | 262 | 72 | 3 | 25 | 283 | 5 | 265 | 1.38e-127 | 363 |
| MsaG014246 | MtrunA17_Chr3g0103201 | 80.455 | 220 | 42 | 1 | 65 | 283 | 1 | 220 | 2.04e-125 | 356 |
| MsaG014246 | MtrunA17_Chr3g0103151 | 77.160 | 162 | 35 | 2 | 1 | 161 | 1 | 161 | 4.86e-86 | 254 |
| MsaG014246 | MtrunA17_Chr3g0103161 | 72.727 | 121 | 33 | 0 | 162 | 282 | 2 | 122 | 1.95e-59 | 185 |
| MsaG014246 | MtrunA17_Chr1g0163071 | 34.589 | 292 | 150 | 8 | 26 | 283 | 2 | 286 | 3.43e-46 | 157 |
| MsaG014246 | MtrunA17_Chr8g0390841 | 33.871 | 310 | 153 | 10 | 10 | 283 | 37 | 330 | 7.18e-42 | 150 |
| MsaG014246 | MtrunA17_Chr8g0390841 | 46.774 | 62 | 32 | 1 | 222 | 283 | 457 | 517 | 1.83e-11 | 64.3 |
| MsaG014246 | MtrunA17_Chr1g0208771 | 33.106 | 293 | 147 | 8 | 25 | 283 | 12 | 289 | 1.58e-40 | 146 |
| MsaG014246 | MtrunA17_Chr1g0208771 | 32.456 | 114 | 72 | 3 | 174 | 283 | 345 | 457 | 5.15e-11 | 62.8 |
| MsaG014246 | MtrunA17_Chr7g0233561 | 33.866 | 313 | 170 | 11 | 1 | 283 | 6 | 311 | 2.08e-40 | 142 |
| MsaG014246 | MtrunA17_Chr3g0131891 | 31.250 | 288 | 184 | 7 | 9 | 283 | 16 | 302 | 2.82e-35 | 129 |
| MsaG014246 | MtrunA17_Chr7g0233521 | 32.331 | 266 | 167 | 5 | 26 | 283 | 2 | 262 | 3.00e-32 | 120 |
| MsaG014246 | MtrunA17_Chr1g0163131 | 30.645 | 310 | 170 | 10 | 2 | 280 | 8 | 303 | 7.53e-32 | 119 |
| MsaG014246 | MtrunA17_Chr4g0067431 | 29.043 | 303 | 182 | 11 | 10 | 283 | 13 | 311 | 3.17e-31 | 118 |
| MsaG014246 | MtrunA17_Chr7g0233531 | 31.734 | 271 | 162 | 6 | 26 | 283 | 2 | 262 | 1.47e-29 | 113 |
| MsaG014246 | MtrunA17_Chr7g0233541 | 28.417 | 278 | 179 | 5 | 26 | 283 | 2 | 279 | 4.24e-29 | 112 |
| MsaG014246 | MtrunA17_Chr1g0163251 | 29.643 | 280 | 178 | 7 | 12 | 282 | 9 | 278 | 2.68e-28 | 112 |
| MsaG014246 | MtrunA17_Chr7g0233571 | 31.651 | 218 | 115 | 4 | 9 | 201 | 7 | 215 | 3.39e-28 | 108 |
| MsaG014246 | MtrunA17_Chr1g0163181 | 29.010 | 293 | 172 | 10 | 14 | 282 | 11 | 291 | 5.87e-28 | 111 |
| MsaG014246 | MtrunA17_Chr1g0163201 | 28.114 | 281 | 164 | 9 | 25 | 282 | 18 | 283 | 8.38e-24 | 99.8 |
| MsaG014246 | MtrunA17_Chr5g0419201 | 33.758 | 157 | 86 | 4 | 12 | 157 | 16 | 165 | 2.61e-22 | 91.7 |
| MsaG014246 | MtrunA17_Chr1g0163261 | 25.177 | 282 | 193 | 5 | 9 | 280 | 6 | 279 | 5.83e-22 | 93.2 |
| MsaG014246 | MtrunA17_Chr1g0163221 | 28.767 | 292 | 180 | 10 | 10 | 283 | 9 | 290 | 9.49e-21 | 90.5 |
| MsaG014246 | MtrunA17_Chr5g0429931 | 27.341 | 267 | 151 | 7 | 25 | 283 | 10 | 241 | 1.51e-20 | 88.2 |
| MsaG014246 | MtrunA17_Chr1g0163231 | 26.990 | 289 | 179 | 12 | 9 | 282 | 6 | 277 | 7.09e-20 | 87.4 |
| MsaG014246 | MtrunA17_Chr3g0077451 | 27.574 | 272 | 145 | 7 | 20 | 283 | 6 | 233 | 8.60e-20 | 85.9 |
| MsaG014246 | MtrunA17_Chr1g0163191 | 37.607 | 117 | 68 | 3 | 12 | 128 | 9 | 120 | 2.22e-19 | 82.8 |
| MsaG014246 | MtrunA17_Chr3g0077461 | 27.106 | 273 | 144 | 7 | 12 | 277 | 237 | 461 | 6.43e-19 | 86.3 |
| MsaG014246 | MtrunA17_Chr3g0077461 | 39.286 | 84 | 51 | 0 | 25 | 108 | 22 | 105 | 6.17e-14 | 71.6 |
| MsaG014246 | MtrunA17_Chr1g0171611 | 24.567 | 289 | 174 | 10 | 9 | 282 | 6 | 265 | 6.63e-18 | 81.6 |
| MsaG014246 | MtrunA17_Chr3g0077421 | 28.205 | 234 | 119 | 5 | 57 | 283 | 4 | 195 | 9.60e-18 | 79.7 |
| MsaG014246 | MtrunA17_Chr3g0077441 | 28.205 | 234 | 119 | 5 | 57 | 283 | 4 | 195 | 9.60e-18 | 79.7 |
| MsaG014246 | MtrunA17_Chr4g0033961 | 32.576 | 132 | 79 | 2 | 12 | 133 | 6 | 137 | 1.20e-17 | 82.4 |
| MsaG014246 | MtrunA17_Chr1g0154161 | 23.929 | 280 | 201 | 7 | 13 | 283 | 30 | 306 | 1.29e-17 | 81.3 |
| MsaG014246 | MtrunA17_Chr6g0467351 | 29.493 | 217 | 119 | 6 | 10 | 201 | 13 | 220 | 2.01e-17 | 79.3 |
| MsaG014246 | MtrunA17_Chr1g0163061 | 42.157 | 102 | 56 | 2 | 10 | 108 | 13 | 114 | 5.69e-17 | 79.0 |
| MsaG014246 | MtrunA17_Chr1g0162441 | 36.111 | 108 | 65 | 2 | 8 | 111 | 1 | 108 | 5.77e-17 | 80.5 |
| MsaG014246 | MtrunA17_Chr1g0163241 | 37.143 | 105 | 58 | 2 | 12 | 116 | 15 | 111 | 2.07e-16 | 75.1 |
| MsaG014246 | MtrunA17_Chr1g0163161 | 42.157 | 102 | 50 | 3 | 38 | 133 | 1 | 99 | 1.00e-15 | 72.4 |
| MsaG014246 | MtrunA17_Chr1g0154131 | 24.315 | 292 | 193 | 9 | 10 | 280 | 182 | 466 | 1.27e-15 | 76.6 |
| MsaG014246 | MtrunA17_Chr3g0077411 | 26.374 | 273 | 139 | 6 | 12 | 277 | 25 | 242 | 2.07e-15 | 75.9 |
| MsaG014246 | MtrunA17_Chr3g0077411 | 25.000 | 288 | 171 | 8 | 10 | 283 | 260 | 516 | 5.30e-15 | 74.7 |
| MsaG014246 | MtrunA17_Chr6g0476181 | 39.474 | 114 | 66 | 2 | 26 | 139 | 2 | 112 | 2.85e-15 | 72.0 |
| MsaG014246 | MtrunA17_Chr1g0163101 | 32.624 | 141 | 84 | 4 | 2 | 134 | 7 | 144 | 2.94e-15 | 72.4 |
| MsaG014246 | MtrunA17_Chr1g0163081 | 39.474 | 114 | 61 | 3 | 26 | 134 | 6 | 116 | 3.32e-15 | 71.2 |
| MsaG014246 | MtrunA17_Chr1g0163121 | 38.261 | 115 | 63 | 3 | 24 | 133 | 9 | 120 | 4.50e-15 | 70.9 |
| MsaG014246 | MtrunA17_Chr1g0162161 | 34.400 | 125 | 74 | 4 | 26 | 145 | 4 | 125 | 5.67e-15 | 70.9 |
| MsaG014246 | MtrunA17_Chr1g0154071 | 23.214 | 336 | 194 | 9 | 6 | 280 | 150 | 482 | 7.29e-15 | 74.3 |
| MsaG014246 | MtrunA17_Chr3g0077381 | 36.458 | 96 | 61 | 0 | 13 | 108 | 23 | 118 | 1.24e-14 | 73.6 |
| MsaG014246 | MtrunA17_Chr3g0077381 | 38.947 | 95 | 56 | 1 | 16 | 108 | 251 | 345 | 6.46e-14 | 71.2 |
| MsaG014246 | MtrunA17_Chr1g0163141 | 39.000 | 100 | 58 | 2 | 24 | 123 | 9 | 105 | 1.35e-13 | 67.8 |
| MsaG014246 | MtrunA17_Chr4g0009491 | 37.895 | 95 | 57 | 1 | 16 | 108 | 229 | 323 | 2.08e-13 | 69.7 |
| MsaG014246 | MtrunA17_Chr4g0009491 | 32.258 | 93 | 63 | 0 | 16 | 108 | 3 | 95 | 3.31e-11 | 63.2 |
| MsaG014246 | MtrunA17_Chr1g0162411 | 35.185 | 108 | 65 | 3 | 5 | 110 | 1 | 105 | 6.34e-13 | 68.6 |
| MsaG014246 | MtrunA17_Chr3g0077361 | 36.634 | 101 | 61 | 2 | 12 | 109 | 40 | 140 | 4.46e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014246 | AT3G18990.1 | 27.059 | 340 | 179 | 9 | 8 | 283 | 1 | 335 | 3.31e-31 | 119 |
| MsaG014246 | AT3G18990.2 | 27.059 | 340 | 179 | 9 | 8 | 283 | 1 | 335 | 7.51e-31 | 119 |
| MsaG014246 | AT1G49475.1 | 36.691 | 139 | 74 | 3 | 11 | 136 | 33 | 170 | 1.81e-18 | 81.6 |
| MsaG014246 | AT4G01580.1 | 35.088 | 114 | 70 | 2 | 11 | 120 | 29 | 142 | 1.28e-15 | 73.9 |
| MsaG014246 | AT3G18960.1 | 37.000 | 100 | 61 | 1 | 11 | 108 | 29 | 128 | 7.32e-15 | 72.0 |
| MsaG014246 | AT3G18960.2 | 37.000 | 100 | 61 | 1 | 11 | 108 | 29 | 128 | 9.82e-15 | 71.6 |
| MsaG014246 | AT1G49480.3 | 30.233 | 129 | 80 | 3 | 160 | 283 | 97 | 220 | 9.73e-14 | 69.3 |
| MsaG014246 | AT1G49480.1 | 30.233 | 129 | 80 | 3 | 160 | 283 | 97 | 220 | 9.73e-14 | 69.3 |
| MsaG014246 | AT1G49480.2 | 30.233 | 129 | 80 | 3 | 160 | 283 | 97 | 220 | 9.73e-14 | 69.3 |
| MsaG014246 | AT4G33280.1 | 23.179 | 302 | 198 | 10 | 10 | 283 | 23 | 318 | 1.29e-12 | 67.4 |
| MsaG014246 | AT3G18960.3 | 39.286 | 84 | 51 | 0 | 25 | 108 | 8 | 91 | 1.62e-12 | 64.7 |
Find 52 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGAATCAGTTGAGATTT+TGG | 0.191329 | 3:-69707476 | MsaT014246.1:CDS |
| GCACTAAATGGAAAGTTTAT+TGG | 0.220986 | 3:-69707705 | MsaT014246.1:CDS |
| AAAGAACTTCATCAATAAAT+TGG | 0.266413 | 3:-69707203 | MsaT014246.1:CDS |
| AGACTACTGATGGCACTAAA+TGG | 0.292817 | 3:-69707717 | MsaT014246.1:CDS |
| AATCTATTTCTAGTGCATTC+TGG | 0.311928 | 3:+69707551 | None:intergenic |
| ACTGATAAACAAGAAAGATT+TGG | 0.358474 | 3:-69706792 | MsaT014246.1:CDS |
| TTCTTCAAACTAATCTTCAT+AGG | 0.362211 | 3:-69708079 | MsaT014246.1:CDS |
| TGGAGAGATTTGGTTTGAAA+AGG | 0.378673 | 3:-69707671 | MsaT014246.1:CDS |
| TTTACTGAAAATTACTCTCT+TGG | 0.385393 | 3:-69707637 | MsaT014246.1:CDS |
| CTCTCTTGGACATGGCTGCT+TGG | 0.402665 | 3:-69707623 | MsaT014246.1:CDS |
| GAAATTACTTCGTTGTTATG+AGG | 0.407954 | 3:-69706900 | MsaT014246.1:CDS |
| GGAGAGATTTGGTTTGAAAA+GGG | 0.411021 | 3:-69707670 | MsaT014246.1:CDS |
| CTACTCATCTGGTAGAAAAT+CGG | 0.421938 | 3:-69707104 | MsaT014246.1:CDS |
| AGTGGAAGTGGACTGAAATC+AGG | 0.429019 | 3:-69706833 | MsaT014246.1:CDS |
| GAAGAATGATCTTGAAGAAA+CGG | 0.439712 | 3:+69708096 | None:intergenic |
| AGAATGTGGTACTGCAGATT+GGG | 0.448452 | 3:-69706940 | MsaT014246.1:CDS |
| AAGTTTGATGTATACATACT+TGG | 0.450789 | 3:-69707574 | MsaT014246.1:CDS |
| TCTCATAAAGCCTACTCATC+TGG | 0.455995 | 3:-69707115 | MsaT014246.1:CDS |
| AAGAATGTGGTACTGCAGAT+TGG | 0.459475 | 3:-69706941 | MsaT014246.1:CDS |
| AGATTTGGTTTGAAAAGGGT+TGG | 0.461112 | 3:-69707666 | MsaT014246.1:CDS |
| AAATTACTTCGTTGTTATGA+GGG | 0.500449 | 3:-69706899 | MsaT014246.1:CDS |
| TCAATAACCCCATTTAAAAG+TGG | 0.503683 | 3:+69706974 | None:intergenic |
| CGACGACATAAGAAAGTTCG+AGG | 0.503833 | 3:-69707400 | MsaT014246.1:intron |
| AAATGGGGTTATTGAGAACA+AGG | 0.504796 | 3:-69706966 | MsaT014246.1:CDS |
| TCTTGGACATGGCTGCTTGG+TGG | 0.517847 | 3:-69707620 | MsaT014246.1:CDS |
| TTGGTGGTGTTTGAATATAA+AGG | 0.518126 | 3:-69707604 | MsaT014246.1:CDS |
| GCCACAGGATGCTAGAGCTC+AGG | 0.519514 | 3:-69707181 | MsaT014246.1:CDS |
| TGGTACTGCAGATTGGGAAA+AGG | 0.536643 | 3:-69706934 | MsaT014246.1:CDS |
| GGCTTAATCGGAAGAAGGCT+AGG | 0.536744 | 3:-69707447 | MsaT014246.1:CDS |
| TCAGTAGTCTTTATCAACAC+TGG | 0.537149 | 3:+69707730 | None:intergenic |
| TGTCTACTAGTAAACTTACT+TGG | 0.541700 | 3:+69707772 | None:intergenic |
| TGTCGTCGAGGAGAAACAAA+AGG | 0.549605 | 3:+69707415 | None:intergenic |
| TGCAGATTGGGAAAAGGTCA+TGG | 0.561983 | 3:-69706928 | MsaT014246.1:CDS |
| AAGTATGTATACATCAAACT+TGG | 0.563066 | 3:+69707576 | None:intergenic |
| GCCAACCCGCGGAAAAGCGA+CGG | 0.564559 | 3:+69706867 | None:intergenic |
| TTCATCAATAAATTGGCCAC+AGG | 0.565636 | 3:-69707196 | MsaT014246.1:CDS |
| ATGATCTTGAAGAAACGGAT+GGG | 0.579896 | 3:+69708101 | None:intergenic |
| TGATCTTGAAGAAACGGATG+GGG | 0.586893 | 3:+69708102 | None:intergenic |
| TGAATGGCTTAATCGGAAGA+AGG | 0.588393 | 3:-69707452 | MsaT014246.1:CDS |
| TTTACTAGTAGACATGGAGT+TGG | 0.590530 | 3:-69707763 | MsaT014246.1:CDS |
| TCCTGAGCTCTAGCATCCTG+TGG | 0.590768 | 3:+69707180 | None:intergenic |
| GATAGAGATTCCTCTAGCCA+TGG | 0.595315 | 3:+69708127 | None:intergenic |
| AATGATCTTGAAGAAACGGA+TGG | 0.610158 | 3:+69708100 | None:intergenic |
| ATCGGAAGAAGGCTAGGCAA+AGG | 0.622199 | 3:-69707441 | MsaT014246.1:CDS |
| CAAACTCCATCCATGGCTAG+AGG | 0.626890 | 3:-69708137 | None:intergenic |
| GAAAATTACTCTCTTGGACA+TGG | 0.632483 | 3:-69707631 | MsaT014246.1:CDS |
| GTGTTGATAAAGACTACTGA+TGG | 0.636125 | 3:-69707727 | MsaT014246.1:CDS |
| AGTAAGTTTACTAGTAGACA+TGG | 0.640204 | 3:-69707769 | MsaT014246.1:CDS |
| TCTTGAAGAAACGGATGGGG+AGG | 0.644974 | 3:+69708105 | None:intergenic |
| CGAACTTTCTTATGTCGTCG+AGG | 0.659272 | 3:+69707403 | None:intergenic |
| TCATCTGGTAGAAAATCGGA+TGG | 0.661350 | 3:-69707100 | MsaT014246.1:intron |
| TGAGAACAAGGATAAGAATG+TGG | 0.664188 | 3:-69706954 | MsaT014246.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 69706769 | 69708147 | 69706769 | ID=MsaG014246 |
| Chr3 | mRNA | 69706769 | 69708147 | 69706769 | ID=MsaT014246.1;Parent=MsaG014246 |
| Chr3 | exon | 69706769 | 69707002 | 69706769 | ID=MsaT014246.1.exon4;Parent=MsaT014246.1 |
| Chr3 | CDS | 69706769 | 69707002 | 69706769 | ID=cds.MsaT014246.1;Parent=MsaT014246.1 |
| Chr3 | exon | 69707101 | 69707246 | 69707101 | ID=MsaT014246.1.exon3;Parent=MsaT014246.1 |
| Chr3 | CDS | 69707101 | 69707246 | 69707101 | ID=cds.MsaT014246.1;Parent=MsaT014246.1 |
| Chr3 | exon | 69707401 | 69707800 | 69707401 | ID=MsaT014246.1.exon2;Parent=MsaT014246.1 |
| Chr3 | CDS | 69707401 | 69707800 | 69707401 | ID=cds.MsaT014246.1;Parent=MsaT014246.1 |
| Chr3 | exon | 69708076 | 69708147 | 69708076 | ID=MsaT014246.1.exon1;Parent=MsaT014246.1 |
| Chr3 | CDS | 69708076 | 69708147 | 69708076 | ID=cds.MsaT014246.1;Parent=MsaT014246.1 |
| Gene Sequence |
| Protein sequence |
