AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014247


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014247 MtrunA17_Chr3g0103191 80.952 252 46 2 1 251 1 251 1.18e-147 414
MsaG014247 MtrunA17_Chr3g0103181 72.887 284 72 3 1 284 1 279 8.14e-147 413
MsaG014247 MtrunA17_Chr3g0103171 69.732 261 78 1 25 284 5 265 2.04e-129 368
MsaG014247 MtrunA17_Chr3g0103201 76.923 221 49 2 65 284 1 220 4.29e-118 338
MsaG014247 MtrunA17_Chr3g0103151 77.640 161 36 0 1 161 1 161 1.02e-89 264
MsaG014247 MtrunA17_Chr3g0103161 66.935 124 40 1 162 285 2 124 3.94e-52 167
MsaG014247 MtrunA17_Chr8g0390841 33.974 312 157 8 10 287 37 333 6.93e-51 175
MsaG014247 MtrunA17_Chr8g0390841 48.438 64 30 2 223 285 457 518 3.12e-11 63.5
MsaG014247 MtrunA17_Chr1g0163071 36.395 294 145 10 26 285 2 287 2.23e-49 166
MsaG014247 MtrunA17_Chr1g0208771 32.886 298 150 8 21 285 10 290 1.12e-44 157
MsaG014247 MtrunA17_Chr1g0208771 26.207 290 166 13 12 285 201 458 9.91e-12 65.1
MsaG014247 MtrunA17_Chr7g0233561 31.561 301 185 6 7 286 13 313 3.73e-42 147
MsaG014247 MtrunA17_Chr3g0131891 31.788 302 169 8 9 286 16 304 6.19e-39 139
MsaG014247 MtrunA17_Chr7g0233521 31.716 268 173 4 25 286 1 264 1.40e-35 129
MsaG014247 MtrunA17_Chr1g0163251 29.682 283 179 8 12 285 9 280 1.10e-30 118
MsaG014247 MtrunA17_Chr5g0429931 30.075 266 154 5 21 285 8 242 1.35e-30 115
MsaG014247 MtrunA17_Chr7g0233541 29.110 292 164 8 26 286 2 281 1.76e-30 116
MsaG014247 MtrunA17_Chr4g0067431 31.949 313 161 13 10 284 13 311 2.26e-30 116
MsaG014247 MtrunA17_Chr7g0233531 30.855 269 172 7 26 286 2 264 1.02e-29 114
MsaG014247 MtrunA17_Chr3g0077451 28.947 266 148 5 24 287 10 236 7.81e-29 110
MsaG014247 MtrunA17_Chr1g0163131 30.063 316 165 12 2 281 8 303 1.63e-28 111
MsaG014247 MtrunA17_Chr7g0233571 37.908 153 93 1 4 154 2 154 8.23e-28 107
MsaG014247 MtrunA17_Chr1g0163261 26.481 287 184 6 9 281 6 279 4.73e-27 107
MsaG014247 MtrunA17_Chr5g0419201 35.333 150 93 2 12 157 16 165 1.56e-26 103
MsaG014247 MtrunA17_Chr1g0163181 28.475 295 172 12 14 283 11 291 1.99e-26 107
MsaG014247 MtrunA17_Chr3g0077411 28.136 295 172 7 1 284 251 516 9.97e-26 106
MsaG014247 MtrunA17_Chr3g0077411 26.199 271 143 5 12 278 25 242 1.08e-20 91.7
MsaG014247 MtrunA17_Chr3g0077461 30.970 268 133 8 12 274 237 457 2.75e-25 104
MsaG014247 MtrunA17_Chr3g0077461 39.450 109 64 1 20 128 19 125 4.67e-19 87.0
MsaG014247 MtrunA17_Chr1g0163221 30.717 293 174 11 10 284 9 290 7.19e-25 102
MsaG014247 MtrunA17_Chr1g0163201 26.056 284 175 8 25 287 18 287 4.73e-24 100
MsaG014247 MtrunA17_Chr1g0162441 36.957 138 75 3 8 133 1 138 2.92e-23 98.6
MsaG014247 MtrunA17_Chr4g0033961 34.848 132 76 2 12 133 6 137 5.41e-22 95.1
MsaG014247 MtrunA17_Chr1g0163231 24.913 289 186 9 9 283 6 277 1.23e-21 92.4
MsaG014247 MtrunA17_Chr3g0077421 28.017 232 125 5 57 285 4 196 1.05e-19 85.1
MsaG014247 MtrunA17_Chr3g0077441 28.017 232 125 5 57 285 4 196 1.05e-19 85.1
MsaG014247 MtrunA17_Chr1g0162411 39.823 113 63 3 7 117 3 112 1.30e-19 88.6
MsaG014247 MtrunA17_Chr3g0077431 27.660 235 125 6 57 287 4 197 3.52e-19 83.6
MsaG014247 MtrunA17_Chr3g0077381 34.483 116 76 0 13 128 23 138 1.20e-18 85.5
MsaG014247 MtrunA17_Chr3g0077381 42.529 87 50 0 22 108 259 345 5.38e-16 77.8
MsaG014247 MtrunA17_Chr1g0171611 23.611 288 179 7 9 283 6 265 1.21e-18 84.0
MsaG014247 MtrunA17_Chr6g0467351 32.692 156 91 3 10 162 13 157 2.40e-18 82.0
MsaG014247 MtrunA17_Chr3g0077361 26.259 278 136 10 12 278 40 259 3.81e-18 84.3
MsaG014247 MtrunA17_Chr4g0009491 34.711 121 68 1 22 131 237 357 5.42e-18 83.6
MsaG014247 MtrunA17_Chr4g0009491 30.973 113 78 0 16 128 3 115 5.67e-15 74.7
MsaG014247 MtrunA17_Chr1g0163241 36.522 115 66 3 15 126 14 124 1.15e-17 78.6
MsaG014247 MtrunA17_Chr1g0163191 31.304 115 75 2 12 126 9 119 1.26e-17 78.6
MsaG014247 MtrunA17_Chr1g0163061 40.385 104 59 2 8 108 11 114 1.40e-17 80.9
MsaG014247 MtrunA17_Chr1g0154161 23.077 286 203 6 13 287 30 309 4.85e-17 80.1
MsaG014247 MtrunA17_Chr6g0476181 41.509 106 59 2 26 131 2 104 5.11e-17 76.6
MsaG014247 MtrunA17_Chr1g0154131 22.222 288 205 6 10 281 182 466 2.16e-16 79.0
MsaG014247 MtrunA17_Chr1g0163161 41.176 102 51 3 38 133 1 99 1.10e-15 72.4
MsaG014247 MtrunA17_Chr1g0163081 39.130 115 60 4 26 134 6 116 1.56e-15 72.4
MsaG014247 MtrunA17_Chr1g0162161 29.545 176 96 7 26 196 4 156 7.55e-15 70.9
MsaG014247 MtrunA17_Chr1g0163121 35.461 141 75 6 24 158 9 139 1.07e-14 70.1
MsaG014247 MtrunA17_Chr1g0163101 30.496 141 87 5 2 134 7 144 1.80e-14 70.5
MsaG014247 MtrunA17_Chr1g0163141 39.583 96 55 2 24 119 9 101 1.85e-14 70.5
MsaG014247 MtrunA17_Chr1g0162421 39.655 58 35 0 24 81 7 64 2.55e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014247 AT3G18990.1 26.765 340 183 10 8 285 1 336 2.64e-34 127
MsaG014247 AT3G18990.2 26.765 340 183 10 8 285 1 336 5.57e-34 127
MsaG014247 AT1G49475.1 38.843 121 70 3 11 129 33 151 3.27e-19 84.0
MsaG014247 AT4G01580.1 32.609 138 85 2 11 146 29 160 2.06e-18 81.6
MsaG014247 AT3G18960.2 39.000 100 59 1 11 108 29 128 1.29e-17 79.7
MsaG014247 AT3G18960.1 39.000 100 59 1 11 108 29 128 1.35e-17 79.7
MsaG014247 AT4G33280.1 25.083 303 194 10 10 285 23 319 9.15e-17 79.7
MsaG014247 AT3G18960.3 41.667 84 49 0 25 108 8 91 6.62e-15 71.6
MsaG014247 AT1G49480.3 29.457 129 84 4 160 285 97 221 2.79e-13 68.2
MsaG014247 AT1G49480.1 29.457 129 84 4 160 285 97 221 2.79e-13 68.2
MsaG014247 AT1G49480.2 29.457 129 84 4 160 285 97 221 2.79e-13 68.2

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACCACATAAGAAAGTTAA+AGG 0.201598 3:+69713336 MsaT014247.1:CDS
TGTCTCCTAGTAAACTTATT+TGG 0.219584 3:-69712964 None:intergenic
GCACTAAATGGAAAGTTTAT+TGG 0.220986 3:+69713031 MsaT014247.1:CDS
TCCTGAGCTCTAGCATCTTT+TGG 0.280232 3:-69720394 None:intergenic
CAATCCTTTGAAGATTAGTT+TGG 0.289306 3:-69712657 None:intergenic
AGACTCCTGATGGCACTAAA+TGG 0.296213 3:+69713019 MsaT014247.1:CDS
CTCTCTTGGACATGGCTGTT+TGG 0.307883 3:+69713113 MsaT014247.1:CDS
GGTGAGATTTGGTTTGAAAA+TGG 0.311641 3:+69713066 MsaT014247.1:CDS
TTTACTGAAAATTACTCTCT+TGG 0.354240 3:+69713099 MsaT014247.1:CDS
AAAGAACTTCATCAATGAAT+TGG 0.387908 3:+69720371 MsaT014247.1:CDS
TATGTAACTGCAGATTTAAA+AGG 0.392361 3:+69720614 MsaT014247.1:CDS
TCTTAGATGAATGGCTTAAT+CGG 0.403352 3:+69713277 MsaT014247.1:CDS
AATCTATTTCCACTGCATTC+CGG 0.411258 3:-69713185 None:intergenic
CACTTGAGATCTTAGATGAA+TGG 0.431142 3:+69713268 MsaT014247.1:CDS
ACTTTCCATTTAGTGCCATC+AGG 0.440549 3:-69713024 None:intergenic
AGTATGTGATTCTGCAGATT+GGG 0.463556 3:+69720657 MsaT014247.1:CDS
AAACCTTTAACTTTCTTATG+TGG 0.464229 3:-69713339 None:intergenic
TCCTTTGAAGATTAGTTTGG+AGG 0.466963 3:-69712654 None:intergenic
ACTTCAAACAGATACATTAA+CGG 0.476013 3:-69720858 None:intergenic
TTCACTTTCGCTTGCAAACA+AGG 0.476076 3:-69720753 None:intergenic
AGATTTGGTTTGAAAATGGT+TGG 0.487548 3:+69713070 MsaT014247.1:CDS
AAATATGTGTACATCAAACT+TGG 0.487598 3:-69713160 None:intergenic
AAATACCAAATAAGTTTACT+AGG 0.488511 3:+69712959 MsaT014247.1:CDS
TTGGTGGTGTTTGAATATAA+AGG 0.491592 3:+69713132 MsaT014247.1:CDS
TGCAGATTGGGAAAAGATCA+TGG 0.498577 3:+69720669 MsaT014247.1:CDS
GATATAATTTCCTTTAGCCA+TGG 0.504996 3:-69712605 None:intergenic
TTAACTTTCTTATGTGGTCG+AGG 0.505436 3:-69713333 None:intergenic
TCTTGGACATGGCTGTTTGG+TGG 0.505898 3:+69713116 MsaT014247.1:CDS
ACTCCATCATCCATGGCTAA+AGG 0.511990 3:+69712595 None:intergenic
TGTGGTCGAGGAGAAGCTAA+TGG 0.512745 3:-69713321 None:intergenic
GCCAAAAGATGCTAGAGCTC+AGG 0.513948 3:+69720393 MsaT014247.1:CDS
CCAGCTTACTATCTACTGTA+TGG 0.515143 3:-69720474 None:intergenic
AATGGTTTCTATCTATCGGC+GGG 0.522564 3:+69720725 MsaT014247.1:CDS
GAGTATGTGATTCTGCAGAT+TGG 0.526064 3:+69720656 MsaT014247.1:CDS
TTGATGTACACATATTTGAC+CGG 0.536461 3:+69713166 MsaT014247.1:CDS
TCAGGAGTCTTTATCAACAC+TGG 0.545485 3:-69713006 None:intergenic
GTTTCTATCTATCGGCGGGT+TGG 0.546695 3:+69720729 MsaT014247.1:CDS
TCCTCCAAACTAATCTTCAA+AGG 0.552905 3:+69712653 MsaT014247.1:CDS
AGCGAAAGTGAACTGCAACC+AGG 0.561940 3:+69720764 MsaT014247.1:CDS
TCAAAGACACAAACATCTCC+TGG 0.563620 3:-69720782 None:intergenic
TGAATGGCTTAATCGGAAGA+AGG 0.573439 3:+69713284 MsaT014247.1:CDS
AAATGGTTTCTATCTATCGG+CGG 0.576380 3:+69720724 MsaT014247.1:CDS
CCATACAGTAGATAGTAAGC+TGG 0.603183 3:+69720474 MsaT014247.1:CDS
TTACTATCTACTGTATGGTG+AGG 0.604910 3:-69720469 None:intergenic
AGGAGTTATTGAGAACAAAG+AGG 0.607683 3:+69720634 MsaT014247.1:CDS
CATATTTGACCGGAATGCAG+TGG 0.622074 3:+69713176 MsaT014247.1:CDS
GAAAATTACTCTCTTGGACA+TGG 0.632483 3:+69713105 MsaT014247.1:CDS
AATAAGTTTACTAGGAGACA+TGG 0.640204 3:+69712967 MsaT014247.1:CDS
GTGTTGATAAAGACTCCTGA+TGG 0.643737 3:+69713009 MsaT014247.1:CDS
ATCGGAAGAAGGCTAGACAA+AGG 0.662228 3:+69713295 MsaT014247.1:CDS
GAAATTGATCTGTTCTAACG+AGG 0.726190 3:+69720697 MsaT014247.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 69712607 69720877 69712607 ID=MsaG014247
Chr3 mRNA 69712607 69720877 69712607 ID=MsaT014247.1;Parent=MsaG014247
Chr3 exon 69712607 69712678 69712607 ID=MsaT014247.1.exon1;Parent=MsaT014247.1
Chr3 CDS 69712607 69712678 69712607 ID=cds.MsaT014247.1;Parent=MsaT014247.1
Chr3 exon 69712958 69713357 69712958 ID=MsaT014247.1.exon2;Parent=MsaT014247.1
Chr3 CDS 69712958 69713357 69712958 ID=cds.MsaT014247.1;Parent=MsaT014247.1
Chr3 exon 69720350 69720495 69720350 ID=MsaT014247.1.exon3;Parent=MsaT014247.1
Chr3 CDS 69720350 69720495 69720350 ID=cds.MsaT014247.1;Parent=MsaT014247.1
Chr3 exon 69720614 69720877 69720614 ID=MsaT014247.1.exon4;Parent=MsaT014247.1
Chr3 CDS 69720614 69720877 69720614 ID=cds.MsaT014247.1;Parent=MsaT014247.1
Gene Sequence

>MsaT014247.1

ATGGCTAAAGGAAATTATATCCTTCCCATCCGTTTCTTCAAAATCATCCTCCAAACTAATCTTCAAAGGATTCAAATACCAAATAAGTTTACTAGGAGACATGGAGTTCATCTATCAAATCCAGTGTTGATAAAGACTCCTGATGGCACTAAATGGAAAGTTTATTGGAAAAAAATCAATGGTGAGATTTGGTTTGAAAATGGTTGGAAAACTTTTACTGAAAATTACTCTCTTGGACATGGCTGTTTGGTGGTGTTTGAATATAAAGGAACTTCCAAGTTTGATGTACACATATTTGACCGGAATGCAGTGGAAATAGATTATGATTCTTCATGTGACACTGATGATGAAAAAGACAATGTTGATCATAGTGATGATGAATCACTTGAGATCTTAGATGAATGGCTTAATCGGAAGAAGGCTAGACAAAGGTCACCATTAGCTTCTCCTCGACCACATAAGAAAGTTAAAGGTGATCATGAGAAGACTACTAAAAGAACTTCATCAATGAATTGGCCAAAAGATGCTAGAGCTCAGGAAGTTGCTCAAAATTTCATCTCAACCAATCCTTTTTTCACAATTCTTGTAAAACCTCACCATACAGTAGATAGTAAGCTGTATGTAACTGCAGATTTAAAAGGAGTTATTGAGAACAAAGAGGAGTATGTGATTCTGCAGATTGGGAAAAGATCATGGAATGTGAAATTGATCTGTTCTAACGAGGCTAAAAATGGTTTCTATCTATCGGCGGGTTGGTCCTTGTTTGCAAGCGAAAGTGAACTGCAACCAGGAGATGTTTGTGTCTTTGAACTGATAAAAAAGAAAGATTTCGTTTTTAAAGTTCATGTCTTCAAAAAAGGGACCGTTAATGTATCTGTTTGA

Protein sequence

>MsaT014247.1

MAKGNYILPIRFFKIILQTNLQRIQIPNKFTRRHGVHLSNPVLIKTPDGTKWKVYWKKINGEIWFENGWKTFTENYSLGHGCLVVFEYKGTSKFDVHIFDRNAVEIDYDSSCDTDDEKDNVDHSDDESLEILDEWLNRKKARQRSPLASPRPHKKVKGDHEKTTKRTSSMNWPKDARAQEVAQNFISTNPFFTILVKPHHTVDSKLYVTADLKGVIENKEEYVILQIGKRSWNVKLICSNEAKNGFYLSAGWSLFASESELQPGDVCVFELIKKKDFVFKVHVFKKGTVNVSV*