AlfalfaGEDB | MsaG014251

M. sativa ssp. caerulea / MsaG014251

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014251	XP_003600453.1	76.316	114	27	0	1	114	171	284	2.08e-56	186

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014251	tr\|G8A2Z4\|G8A2Z4_MEDTR	76.316	114	27	0	1	114	171	284	9.94e-57	186

TFs/TRs:

Gene ID	Type	Classification
MsaG014251	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000118	MsaG014251	0.825563	7.776034e-54	1.986638e-51
MsaG000523	MsaG014251	0.812238	8.055261e-51	1.453033e-48
MsaG001390	MsaG014251	0.804969	2.826871e-49	4.282642e-47
MsaG004084	MsaG014251	0.811183	1.363132e-50	2.396032e-48
MsaG004902	MsaG014251	0.820511	1.157251e-52	2.579858e-50
MsaG005394	MsaG014251	0.805457	2.236745e-49	3.427221e-47
MsaG006119	MsaG014251	0.822397	4.267218e-53	1.000193e-50
MsaG006979	MsaG014251	0.817688	5.043807e-52	1.044277e-49
MsaG010497	MsaG014251	0.804346	3.808823e-49	5.687624e-47
MsaG013266	MsaG014251	0.824158	1.662167e-53	4.086106e-51
MsaG014125	MsaG014251	0.965793	1.763034e-124	1.405567e-118
MsaG014251	MsaG018121	0.802580	8.810202e-49	1.263014e-46
MsaG014251	MsaG018974	0.819207	2.291817e-52	4.936368e-50
MsaG014251	MsaG027237	0.806527	1.335960e-49	2.099268e-47
MsaG014251	MsaG040952	0.827859	2.215041e-54	6.033343e-52
MsaG014251	MsaG042772	0.806207	1.558807e-49	2.431079e-47
MsaG014251	MsaG045506	0.810896	1.572245e-50	2.744208e-48
MsaG014251	MsaG045980	0.822164	4.828899e-53	1.124767e-50
MsaG014251	MsaG046794	0.808712	4.611861e-50	7.635257e-48
MsaG014251	MsaG002695	0.813447	4.391892e-51	8.165097e-49
MsaG014251	MsaG008516	0.818106	4.063137e-52	8.503910e-50
MsaG014251	MsaG026065	0.818928	2.649911e-52	5.665970e-50
MsaG014251	MsaG031431	0.819830	1.654409e-52	3.622111e-50
MsaG014251	MsaG036797	0.807342	9.000048e-50	1.441681e-47
MsaG014251	MsaG041605	0.800590	2.244699e-48	3.075728e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014251	MtrunA17_Chr3g0103201	74.561	114	29	0	1	114	107	220	3.60e-57	176
MsaG014251	MtrunA17_Chr3g0103161	69.231	117	36	0	2	118	11	127	2.48e-55	168
MsaG014251	MtrunA17_Chr3g0103181	69.912	113	34	0	2	114	167	279	6.91e-51	162
MsaG014251	MtrunA17_Chr3g0103171	63.793	116	40	1	1	114	150	265	1.97e-44	145
MsaG014251	MtrunA17_Chr3g0103191	69.512	82	24	1	1	81	170	251	3.68e-32	113
MsaG014251	MtrunA17_Chr8g0390841	52.381	63	29	1	53	115	457	518	6.59e-16	72.4
MsaG014251	MtrunA17_Chr8g0390841	33.333	114	66	3	5	115	225	331	3.20e-13	64.7
MsaG014251	MtrunA17_Chr1g0208771	36.522	115	68	3	5	115	345	458	1.76e-15	70.9
MsaG014251	MtrunA17_Chr1g0208771	34.862	109	59	6	13	117	192	292	1.08e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAATAACCCCTTTAAAATC+TGG	0.344940	3:+69753829	None:intergenic
ACTGATAAACAAGAAAGATT+TGG	0.358474	3:-69753647	MsaT014251.1:CDS
CGTTGTTACGAAGGCAGATA+TGG	0.391650	3:-69753745	MsaT014251.1:CDS
ATAGAACTTCATCAATGAAT+TGG	0.394729	3:-69754052	None:intergenic
AGTGAGAGTGGACTGGAATC+AGG	0.399530	3:-69753688	MsaT014251.1:CDS
TCTCGAGCTCTAGCATCCTT+TGG	0.429549	3:+69754029	None:intergenic
AGAATGTGATGATGCAGATT+AGG	0.440524	3:-69753795	MsaT014251.1:CDS
AAACTTAATCATATAGAAGA+TGG	0.505339	3:-69753957	MsaT014251.1:CDS
GTCGTCGCCTTTCCGTGGGT+TGG	0.516583	3:-69753723	MsaT014251.1:CDS
TGATGATGCAGATTAGGAAA+AGG	0.518118	3:-69753789	MsaT014251.1:CDS
TCATATAGAAGATGGTCAGC+TGG	0.541802	3:-69753949	MsaT014251.1:intron
TAAAGGGGTTATTGAGAACA+AGG	0.546960	3:-69753821	MsaT014251.1:CDS
TATGGTCGTCGCCTTTCCGT+GGG	0.562523	3:-69753727	MsaT014251.1:CDS
ATATGGTCGTCGCCTTTCCG+TGG	0.567959	3:-69753728	MsaT014251.1:CDS
TCATTGTTTGCAAGTGAGAG+TGG	0.589705	3:-69753700	MsaT014251.1:CDS
TTCATCAATGAATTGGCCAA+AGG	0.600242	3:-69754045	None:intergenic
ACAATGACCAACCCACGGAA+AGG	0.604310	3:+69753716	None:intergenic
AAATTACTCCGTTGTTACGA+AGG	0.605850	3:-69753754	MsaT014251.1:CDS
GTTTGCAAGTGAGAGTGGAC+TGG	0.609003	3:-69753695	MsaT014251.1:CDS
TGCAGATTAGGAAAAGGTCA+TGG	0.613419	3:-69753783	MsaT014251.1:CDS
TGCAAACAATGACCAACCCA+CGG	0.632224	3:+69753711	None:intergenic
CATATCTGCCTTCGTAACAA+CGG	0.666214	3:+69753746	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	69753612	69754060	69753612	ID=MsaG014251
Chr3	mRNA	69753612	69754060	69753612	ID=MsaT014251.1;Parent=MsaG014251
Chr3	exon	69753612	69753857	69753612	ID=MsaT014251.1.exon2;Parent=MsaT014251.1
Chr3	CDS	69753612	69753857	69753612	ID=cds.MsaT014251.1;Parent=MsaT014251.1
Chr3	exon	69753950	69754060	69753950	ID=MsaT014251.1.exon1;Parent=MsaT014251.1
Chr3	CDS	69753950	69754060	69753950	ID=cds.MsaT014251.1;Parent=MsaT014251.1

Gene Sequence	>MsaT014251.1 ATGAATTGGCCAAAGGATGCTAGAGCTCGAGAAGTAGCTGCGAAATTCATCTCAAGCAATCCTTTTTTCACAATTCTCATAAAACTTAATCATATAGAAGATGGTCAGCTGTCTGTACCAGATTTTAAAGGGGTTATTGAGAACAAGGACAAGAATGTGATGATGCAGATTAGGAAAAGGTCATGGAATGTGAAATTACTCCGTTGTTACGAAGGCAGATATGGTCGTCGCCTTTCCGTGGGTTGGTCATTGTTTGCAAGTGAGAGTGGACTGGAATCAGGAGATGTTTGTGTCTTTGAACTGATAAACAAGAAAGATTTGGTTTTTAAAGTTCATATTTTCAAAAGTGACGGTTAA
Protein sequence	>MsaT014251.1 MNWPKDARAREVAAKFISSNPFFTILIKLNHIEDGQLSVPDFKGVIENKDKNVMMQIRKRSWNVKLLRCYEGRYGRRLSVGWSLFASESGLESGDVCVFELINKKDLVFKVHIFKSDG*