AlfalfaGEDB | MsaG014253

M. sativa ssp. caerulea / MsaG014253

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014253	XP_003600451.1	77.333	225	45	1	1	219	1	225	1.45e-122	358

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014253	sp\|Q9XIB4\|Y1475_ARATH	37.162	148	79	3	11	145	33	179	7.06e-19	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014253	tr\|G8A2Z6\|G8A2Z6_MEDTR	77.333	225	45	1	1	219	1	225	6.91e-123	358

TFs/TRs:

Gene ID	Type	Classification
MsaG014253	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014253	MtrunA17_Chr3g0103191	77.333	225	45	1	1	219	1	225	1.33e-126	358
MsaG014253	MtrunA17_Chr3g0103181	73.206	209	50	4	1	208	1	204	7.24e-105	304
MsaG014253	MtrunA17_Chr3g0103171	71.910	178	48	2	25	201	5	181	1.11e-88	262
MsaG014253	MtrunA17_Chr3g0103151	77.160	162	35	2	1	161	1	161	2.26e-87	255
MsaG014253	MtrunA17_Chr3g0103201	72.671	161	38	2	65	219	1	161	1.10e-76	230
MsaG014253	MtrunA17_Chr7g0233571	32.000	225	119	3	4	203	2	217	3.19e-36	127
MsaG014253	MtrunA17_Chr7g0233561	36.150	213	111	5	9	202	15	221	4.38e-36	129
MsaG014253	MtrunA17_Chr1g0163071	34.579	214	109	5	26	208	2	215	1.84e-34	125
MsaG014253	MtrunA17_Chr8g0390841	36.264	182	106	3	10	188	37	211	4.38e-32	122
MsaG014253	MtrunA17_Chr3g0131891	32.692	208	131	3	9	209	16	221	1.34e-31	118
MsaG014253	MtrunA17_Chr7g0233521	35.204	196	110	5	25	214	1	185	1.61e-30	114
MsaG014253	MtrunA17_Chr1g0208771	32.569	218	108	4	25	212	12	220	6.19e-28	110
MsaG014253	MtrunA17_Chr7g0233541	30.688	189	119	1	26	202	2	190	3.39e-26	102
MsaG014253	MtrunA17_Chr7g0233531	32.768	177	115	2	26	202	2	174	8.62e-25	99.0
MsaG014253	MtrunA17_Chr5g0419201	32.667	150	97	2	12	157	16	165	2.72e-24	95.5
MsaG014253	MtrunA17_Chr4g0067431	38.983	118	69	2	10	124	13	130	1.38e-23	96.7
MsaG014253	MtrunA17_Chr1g0163251	32.653	196	115	6	12	200	9	194	6.49e-23	95.5
MsaG014253	MtrunA17_Chr1g0163181	28.230	209	132	6	14	208	11	215	7.95e-21	90.1
MsaG014253	MtrunA17_Chr1g0163131	34.532	139	82	4	2	134	8	143	4.72e-20	86.7
MsaG014253	MtrunA17_Chr1g0163191	37.143	105	62	2	12	116	9	109	5.84e-20	83.2
MsaG014253	MtrunA17_Chr1g0162441	37.037	108	64	2	8	111	1	108	1.25e-19	86.7
MsaG014253	MtrunA17_Chr1g0163261	35.484	124	70	3	9	124	6	127	2.68e-19	84.3
MsaG014253	MtrunA17_Chr1g0171611	36.937	111	66	2	9	117	6	114	3.64e-19	84.0
MsaG014253	MtrunA17_Chr1g0163231	34.058	138	86	3	9	144	6	140	5.17e-19	83.6
MsaG014253	MtrunA17_Chr1g0163061	41.346	104	58	2	8	108	11	114	2.07e-18	81.6
MsaG014253	MtrunA17_Chr4g0033961	30.303	132	82	2	12	133	6	137	3.45e-18	82.4
MsaG014253	MtrunA17_Chr1g0163121	39.130	115	62	3	24	133	9	120	6.62e-18	77.4
MsaG014253	MtrunA17_Chr1g0163241	34.167	120	67	3	12	127	15	126	8.75e-18	77.8
MsaG014253	MtrunA17_Chr6g0467351	36.697	109	66	2	10	115	13	121	1.00e-17	79.0
MsaG014253	MtrunA17_Chr6g0476181	41.509	106	59	2	26	131	2	104	2.46e-17	76.3
MsaG014253	MtrunA17_Chr1g0163081	39.474	114	61	3	26	134	6	116	3.54e-17	75.5
MsaG014253	MtrunA17_Chr1g0163101	32.624	141	84	4	2	134	7	144	1.00e-16	75.1
MsaG014253	MtrunA17_Chr3g0077411	41.414	99	56	1	12	108	25	123	2.16e-16	77.4
MsaG014253	MtrunA17_Chr3g0077411	27.700	213	123	5	1	208	251	437	4.23e-15	73.9
MsaG014253	MtrunA17_Chr1g0162161	39.091	110	59	4	26	130	4	110	3.02e-16	73.6
MsaG014253	MtrunA17_Chr1g0163201	34.783	92	58	1	25	116	18	107	3.96e-16	76.6
MsaG014253	MtrunA17_Chr3g0077451	28.947	190	99	4	20	208	6	160	4.42e-16	74.7
MsaG014253	MtrunA17_Chr5g0429931	30.899	178	109	4	25	199	10	176	6.39e-16	74.3
MsaG014253	MtrunA17_Chr3g0077381	34.454	119	75	1	13	128	23	141	6.67e-16	75.9
MsaG014253	MtrunA17_Chr3g0077381	37.895	95	57	1	16	108	251	345	7.44e-13	67.0
MsaG014253	MtrunA17_Chr1g0163161	41.176	102	51	3	38	133	1	99	6.94e-16	71.6
MsaG014253	MtrunA17_Chr3g0077461	41.414	99	56	1	12	108	237	335	9.12e-16	75.5
MsaG014253	MtrunA17_Chr3g0077461	37.736	106	61	2	7	108	1	105	9.52e-15	72.8
MsaG014253	MtrunA17_Chr1g0162411	36.449	107	63	3	7	111	3	106	2.26e-15	74.3
MsaG014253	MtrunA17_Chr1g0163141	39.000	100	58	2	24	123	9	105	9.01e-15	70.1
MsaG014253	MtrunA17_Chr4g0009491	38.947	95	56	1	16	108	229	323	2.03e-14	71.6
MsaG014253	MtrunA17_Chr4g0009491	30.172	116	78	1	16	128	3	118	7.61e-12	63.9
MsaG014253	MtrunA17_Chr1g0163221	34.314	102	64	2	10	108	9	110	1.85e-13	68.6
MsaG014253	MtrunA17_Chr3g0077361	38.614	101	59	2	12	109	40	140	1.88e-13	68.9
MsaG014253	MtrunA17_Chr3g0103161	54.902	51	23	0	162	212	2	52	2.05e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG014253	AT1G49475.1	37.162	148	79	3	11	145	33	179	7.17e-20	84.3
MsaG014253	AT3G18990.1	27.273	253	125	8	8	201	1	253	3.48e-18	82.4
MsaG014253	AT3G18990.2	27.273	253	125	8	8	201	1	253	4.59e-18	82.4
MsaG014253	AT4G01580.1	34.058	138	83	3	11	146	29	160	6.09e-17	76.3
MsaG014253	AT3G18960.1	39.000	100	59	1	11	108	29	128	2.42e-16	75.1
MsaG014253	AT3G18960.2	39.000	100	59	1	11	108	29	128	2.80e-16	74.7
MsaG014253	AT3G18960.3	41.667	84	49	0	25	108	8	91	6.49e-14	67.8

Find 42 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACTAAATGGAAAATTTAT+TGG	0.195816	3:+69860640	MsaT014253.1:CDS
TGTCTCTTTGTAAACTTTCT+TGG	0.219902	3:-69860573	None:intergenic
AGACTCCTGATGACACTAAA+TGG	0.267004	3:+69860628	MsaT014253.1:CDS
AATCTATTTCTACCGCATTC+TGG	0.299695	3:-69860794	None:intergenic
TTGATTTACTTCCAGATGAT+TGG	0.327760	3:-69861217	None:intergenic
CGACCACACAAGAAAGTTAG+AGG	0.346342	3:+69860945	MsaT014253.1:CDS
TTTACTGAAAATTACTCTCT+TGG	0.379721	3:+69860708	MsaT014253.1:CDS
TCCTTCAAACTAATCTTCAT+AGG	0.391228	3:+69860261	MsaT014253.1:CDS
TCCTGAGCTCTAACATCCTT+TGG	0.395829	3:-69861141	None:intergenic
TGATTTACTTCCAGATGATT+GGG	0.400747	3:-69861216	None:intergenic
CTCTCTTGGACATGGCTGCT+TGG	0.402665	3:+69860722	MsaT014253.1:CDS
TCCTATGAAGATTAGTTTGA+AGG	0.404224	3:-69860262	None:intergenic
TCTCATACAGCCCAATCATC+TGG	0.413662	3:+69861206	MsaT014253.1:CDS
AGATTTGGTTTGAAAGGGGT+TGG	0.435761	3:+69860679	MsaT014253.1:CDS
TGGTGAGATTTGGTTTGAAA+GGG	0.445670	3:+69860674	MsaT014253.1:CDS
AAAGAACTTCATCAATGAAT+CGG	0.459762	3:+69861118	MsaT014253.1:CDS
ATGGTGAGATTTGGTTTGAA+AGG	0.464155	3:+69860673	MsaT014253.1:CDS
TCAGGAGTCTTTATCACCAC+TGG	0.464260	3:-69860615	None:intergenic
AAGTTTGATGTACACATACT+TGG	0.471543	3:+69860771	MsaT014253.1:CDS
TCATCTGGAAGTAAATCAAC+TGG	0.475811	3:+69861221	MsaT014253.1:CDS
GCCAAAGGATGTTAGAGCTC+AGG	0.500995	3:+69861140	MsaT014253.1:CDS
CTAACTTTCTTGTGTGGTCG+AGG	0.505612	3:-69860942	None:intergenic
TCTTGGACATGGCTGCTTGG+TGG	0.517847	3:+69860725	MsaT014253.1:CDS
TTGGTGGTGTTTGAATATAA+AGG	0.518126	3:+69860741	MsaT014253.1:CDS
GTCACAGAAACCTACGGCGG+TGG	0.529458	3:+69863066	MsaT014253.1:CDS
TGTGGTCGAGGAGAAACAAA+AGG	0.547165	3:-69860930	None:intergenic
AAGTATGTGTACATCAAACT+TGG	0.555627	3:-69860769	None:intergenic
TGTATGAGAACTGTGAAGAA+AGG	0.560242	3:-69861192	None:intergenic
CATAGAATTTCCTCTAGCCA+TGG	0.569082	3:-69860213	None:intergenic
TGAATGGCTGAATCAGAAGA+AGG	0.584375	3:+69860893	MsaT014253.1:CDS
TTCATCAATGAATCGGCCAA+AGG	0.600573	3:+69861125	MsaT014253.1:CDS
TTTACAAAGAGACATGGAGT+TGG	0.622876	3:+69860582	MsaT014253.1:CDS
AAACCTCTAACTTTCTTGTG+TGG	0.625711	3:-69860948	None:intergenic
GAAAATTACTCTCTTGGACA+TGG	0.632483	3:+69860714	MsaT014253.1:CDS
GGTGAGATTTGGTTTGAAAG+GGG	0.635152	3:+69860675	MsaT014253.1:CDS
ATCACAGTCACAGAAACCTA+CGG	0.646469	3:+69863060	MsaT014253.1:CDS
ACTCTTTCATCCATGGCTAG+AGG	0.653576	3:+69860203	None:intergenic
AGAAAGTTTACAAAGAGACA+TGG	0.659306	3:+69860576	MsaT014253.1:CDS
AGTTGGTCTATCAAATCCAG+TGG	0.699067	3:+69860599	MsaT014253.1:CDS
ACACATACTTGGCCAGAATG+CGG	0.704931	3:+69860782	MsaT014253.1:CDS
TGTGAAGAAAGGATTGCATG+AGG	0.721141	3:-69861181	None:intergenic
ACAGTCACAGAAACCTACGG+CGG	0.815984	3:+69863063	MsaT014253.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	69860215	69863101	69860215	ID=MsaG014253
Chr3	mRNA	69860215	69863101	69860215	ID=MsaT014253.1;Parent=MsaG014253
Chr3	exon	69860215	69860286	69860215	ID=MsaT014253.1.exon1;Parent=MsaT014253.1
Chr3	CDS	69860215	69860286	69860215	ID=cds.MsaT014253.1;Parent=MsaT014253.1
Chr3	exon	69860567	69860966	69860567	ID=MsaT014253.1.exon2;Parent=MsaT014253.1
Chr3	CDS	69860567	69860966	69860567	ID=cds.MsaT014253.1;Parent=MsaT014253.1
Chr3	exon	69861097	69861242	69861097	ID=MsaT014253.1.exon3;Parent=MsaT014253.1
Chr3	CDS	69861097	69861242	69861097	ID=cds.MsaT014253.1;Parent=MsaT014253.1
Chr3	exon	69863030	69863101	69863030	ID=MsaT014253.1.exon4;Parent=MsaT014253.1
Chr3	CDS	69863030	69863101	69863030	ID=cds.MsaT014253.1;Parent=MsaT014253.1

Gene Sequence

>MsaT014253.1

ATGGCTAGAGGAAATTCTATGCTTCCCATCCGTTTCTTCAAAATCATCCTTCAAACTAATCTTCATAGGATTCAAATACCAAGAAAGTTTACAAAGAGACATGGAGTTGGTCTATCAAATCCAGTGGTGATAAAGACTCCTGATGACACTAAATGGAAAATTTATTGGAAAAAAATCAATGGTGAGATTTGGTTTGAAAGGGGTTGGAAATGTTTTACTGAAAATTACTCTCTTGGACATGGCTGCTTGGTGGTGTTTGAATATAAAGGAACTTCCAAGTTTGATGTACACATACTTGGCCAGAATGCGGTAGAAATAGATTATTCATCAAGCGACACTGATGATGAAAATGACAATGTTGATCATAGTGATGATGATGAATCAGTTGAGTTTTTGGATGAATGGCTGAATCAGAAGAAGGCTTCAAAAAGATTACCTTTTGTTTCTCCTCGACCACACAAGAAAGTTAGAGGTGATCATGAGAAGAGTGCTAAAAGAACTTCATCAATGAATCGGCCAAAGGATGTTAGAGCTCAGGAAGTAGCTCAAAATTTCACCTCATGCAATCCTTTCTTCACAGTTCTCATACAGCCCAATCATCTGGAAGTAAATCAACTGTTTGTTGTTGTCATTAATAAAAAGTGCAACATCACAGTCACAGAAACCTACGGCGGTGGAAAAAATAGTTAG

Protein sequence

>MsaT014253.1

MARGNSMLPIRFFKIILQTNLHRIQIPRKFTKRHGVGLSNPVVIKTPDDTKWKIYWKKINGEIWFERGWKCFTENYSLGHGCLVVFEYKGTSKFDVHILGQNAVEIDYSSSDTDDENDNVDHSDDDESVEFLDEWLNQKKASKRLPFVSPRPHKKVRGDHEKSAKRTSSMNRPKDVRAQEVAQNFTSCNPFFTVLIQPNHLEVNQLFVVVINKKCNITVTETYGGGKNS*