AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014267


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014267 MtrunA17_Chr3g0103391 98.784 329 4 0 1 329 1 329 0.0 666
MsaG014267 MtrunA17_Chr8g0352251 46.445 211 87 7 20 226 21 209 1.33e-46 159
MsaG014267 MtrunA17_Chr2g0300261 60.577 104 36 3 128 226 30 133 1.22e-35 129
MsaG014267 MtrunA17_Chr2g0292411 56.731 104 40 1 144 242 81 184 1.58e-32 120
MsaG014267 MtrunA17_Chr8g0343451 54.717 106 44 2 124 226 116 220 2.81e-32 122
MsaG014267 MtrunA17_Chr7g0267581 41.579 190 88 8 104 285 106 280 2.04e-30 117
MsaG014267 MtrunA17_Chr2g0329811 49.624 133 52 3 112 229 114 246 2.68e-30 117
MsaG014267 MtrunA17_Chr1g0177091 50.427 117 44 4 124 237 126 231 1.20e-27 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014267 AT5G12840.4 55.859 256 105 6 16 268 17 267 9.78e-86 259
MsaG014267 AT5G12840.2 55.859 256 105 6 16 268 17 267 9.78e-86 259
MsaG014267 AT5G12840.5 55.642 257 105 7 16 268 17 268 6.14e-84 255
MsaG014267 AT5G12840.3 55.642 257 105 7 16 268 17 268 6.14e-84 255
MsaG014267 AT5G12840.1 55.642 257 105 7 16 268 17 268 6.14e-84 255
MsaG014267 AT3G20910.1 59.361 219 59 7 116 329 110 303 5.27e-74 231
MsaG014267 AT1G30500.1 59.091 110 40 3 124 231 52 158 3.15e-33 121
MsaG014267 AT1G30500.2 56.757 111 45 2 124 231 52 162 3.93e-32 119
MsaG014267 AT1G72830.1 57.143 98 39 2 132 226 137 234 4.01e-31 120
MsaG014267 AT2G34720.1 45.714 140 70 2 125 258 48 187 5.76e-31 116
MsaG014267 AT2G34720.2 47.015 134 65 2 131 258 19 152 5.02e-30 112
MsaG014267 AT2G34720.3 47.015 134 65 2 131 258 19 152 5.02e-30 112
MsaG014267 AT1G17590.3 57.732 97 35 3 132 226 135 227 8.10e-30 116
MsaG014267 AT1G17590.2 57.732 97 35 3 132 226 135 227 8.10e-30 116
MsaG014267 AT1G17590.4 57.732 97 35 3 132 226 135 227 8.10e-30 116
MsaG014267 AT1G17590.1 57.732 97 35 3 132 226 135 227 8.10e-30 116
MsaG014267 AT1G72830.2 56.566 99 39 3 132 226 137 235 1.94e-29 115
MsaG014267 AT1G54160.1 43.066 137 68 3 95 226 102 233 8.75e-29 113
MsaG014267 AT3G14020.2 54.167 96 39 3 135 226 132 226 6.44e-27 108
MsaG014267 AT3G14020.1 54.167 96 39 3 135 226 132 226 6.44e-27 108
MsaG014267 AT5G06510.1 31.776 214 110 8 27 235 13 195 9.40e-22 93.2
MsaG014267 AT5G06510.5 31.776 214 110 8 27 235 13 195 9.40e-22 93.2
MsaG014267 AT5G06510.2 31.776 214 110 8 27 235 13 195 9.40e-22 93.2
MsaG014267 AT5G06510.6 31.776 214 110 8 27 235 13 195 9.40e-22 93.2
MsaG014267 AT5G06510.4 45.872 109 43 4 130 235 5 100 1.25e-21 90.5
MsaG014267 AT5G06510.7 45.872 109 43 4 130 235 5 100 1.25e-21 90.5
MsaG014267 AT5G06510.3 45.872 109 43 4 130 235 51 146 5.59e-21 90.1
MsaG014267 AT3G05690.1 44.928 138 55 6 114 242 88 213 8.28e-21 91.3
MsaG014267 AT3G05690.2 34.468 235 110 11 22 242 46 250 1.48e-20 91.3
MsaG014267 AT1G72830.3 48.421 95 27 2 132 226 137 209 6.74e-19 86.3

Find 106 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTATCAGGATCCATATTA+TGG 0.185298 3:-70160962 MsaT014267.1:CDS
CCTCGGTTGATTTGCAGTTT+CGG 0.272870 3:+70161726 None:intergenic
TTCCTCATTCATCTCACTAT+TGG 0.285307 3:+70161543 None:intergenic
TTGATTTGCAGTTTCGGACT+TGG 0.313960 3:+70161732 None:intergenic
CTCAAGGGGCAAAGGCATTC+TGG 0.314340 3:+70160752 None:intergenic
GTGGAAGAAGGGGACTGTTC+AGG 0.315135 3:-70159533 MsaT014267.1:CDS
GCATGAGGCACTCCTATAAA+AGG 0.333358 3:+70160774 None:intergenic
TATGCAATCTTCAAGGTATC+AGG 0.338770 3:-70159564 MsaT014267.1:CDS
TTCTAAAGCACCTGATTGAT+TGG 0.345684 3:+70161483 None:intergenic
CCAGACATTGTTTGCGCTAC+AGG 0.346287 3:+70161634 None:intergenic
TGTTTAGCGTTCACATAAAC+AGG 0.347626 3:+70160717 None:intergenic
CAAAGACTGGCCTTCCTCAT+TGG 0.355087 3:+70161567 None:intergenic
TCGCATGTTGGGAGAATTCC+AGG 0.360834 3:+70159646 None:intergenic
GCAAGAGGATCAAACGAAAA+TGG 0.361215 3:-70159617 MsaT014267.1:CDS
TTCTTCTTGATAGGAAATTA+CGG 0.368800 3:-70161327 MsaT014267.1:intron
GCATACCATTGTTGTTGTTA+TGG 0.371144 3:+70159582 None:intergenic
ATGTGCTACAAATCCTTATC+AGG 0.378434 3:-70160975 MsaT014267.1:CDS
CTTGCATCTTGTCGCATGTT+GGG 0.394811 3:+70159635 None:intergenic
CTTATCAGGATCCATATTAT+GGG 0.407754 3:-70160961 MsaT014267.1:CDS
TCGCAATCAATTAGTTCATC+TGG 0.408092 3:-70159701 MsaT014267.1:CDS
GAAAACTGATGGTGAGGCCT+CGG 0.419006 3:-70159756 MsaT014267.1:CDS
GAGGCCTCGGGAAAGGATAA+TGG 0.429841 3:-70159743 MsaT014267.1:CDS
CCTGTAGCGCAAACAATGTC+TGG 0.435636 3:-70161634 MsaT014267.1:CDS
ACAATCAAGAGAGGATGAAT+TGG 0.445592 3:+70161609 None:intergenic
TCCTCTCTTGATTGTCCCAA+TGG 0.445841 3:-70161601 MsaT014267.1:CDS
TGTCTAGATTCATGAAGATA+TGG 0.448950 3:+70159827 None:intergenic
CCATAATATGGATCCTGATA+AGG 0.451848 3:+70160962 None:intergenic
CTTCAGACTCTGCGGAAACC+TGG 0.454407 3:-70159664 MsaT014267.1:CDS
TCTAAAGCACCTGATTGATT+GGG 0.457401 3:+70161484 None:intergenic
CCTCTGCGATGCCTCGTTCG+AGG 0.460703 3:+70159490 None:intergenic
TCTTGCATCTTGTCGCATGT+TGG 0.462937 3:+70159634 None:intergenic
TGATTTGCAGTTTCGGACTT+GGG 0.466356 3:+70161733 None:intergenic
TGGAAGAAGGGGACTGTTCA+GGG 0.467131 3:-70159532 MsaT014267.1:CDS
TTGAGAAGGGCAAGAGGCAC+CGG 0.467176 3:-70159794 MsaT014267.1:CDS
CCTGCTGCTCCCAATCAATC+AGG 0.469165 3:-70161493 MsaT014267.1:intron
CCTGATTGATTGGGAGCAGC+AGG 0.471231 3:+70161493 None:intergenic
AATGCCTTTGCCCCTTGAGA+TGG 0.476507 3:-70160748 MsaT014267.1:CDS
GTCTTCGCCTCAGAATTCCT+TGG 0.480874 3:+70160691 None:intergenic
ATTGATTGCGACGACAAGAC+TGG 0.480972 3:+70159713 None:intergenic
ACTCCTCAGCTGGAACTTGT+TGG 0.498120 3:-70161240 MsaT014267.1:CDS
TCGGGAAAGGATAATGGCTC+TGG 0.498937 3:-70159737 MsaT014267.1:CDS
ATTGGATTCTGAATCACCAT+TGG 0.502864 3:+70161585 None:intergenic
CAGGGTGAAAGAGTGGAAGA+AGG 0.503255 3:-70159545 MsaT014267.1:CDS
TTGGATTCTGAATCACCATT+GGG 0.505994 3:+70161586 None:intergenic
TTTCTTTGCAAATCGTCCTC+CGG 0.509061 3:+70159775 None:intergenic
AAAACTGATGGTGAGGCCTC+GGG 0.514204 3:-70159755 MsaT014267.1:CDS
GAACCTTGGTGGCGTGGTGT+TGG 0.516801 3:-70161664 MsaT014267.1:CDS
CCTCACACAGACTCCTCAGC+TGG 0.517659 3:-70161250 MsaT014267.1:CDS
GAAGCTCATAAAATCTAGAA+AGG 0.519482 3:-70160640 MsaT014267.1:intron
GTGCTAAAGCAGAGCTTGAA+AGG 0.520912 3:-70160662 MsaT014267.1:CDS
ACCATTGTTGTTGTTATGGT+AGG 0.521132 3:+70159586 None:intergenic
AGGGTGAAAGAGTGGAAGAA+GGG 0.525419 3:-70159544 MsaT014267.1:CDS
ACAATGGTATGCAATCTTCA+AGG 0.528717 3:-70159571 MsaT014267.1:CDS
TGATGGTGAGGCCTCGGGAA+AGG 0.531610 3:-70159750 MsaT014267.1:CDS
GTGGTGTTTATTCTGAACCT+TGG 0.535143 3:-70161678 MsaT014267.1:CDS
CAGGTTCTTGAGCCATCTCA+AGG 0.541180 3:+70160736 None:intergenic
AGCTTATCCTCACCAGCCGT+TGG 0.544838 3:-70160927 MsaT014267.1:intron
TCTCACTATTGGACAAAGAC+TGG 0.550556 3:+70161554 None:intergenic
GACATCAGCATGCTTTGAGA+AGG 0.559068 3:-70159808 MsaT014267.1:CDS
TGAGCCATCTCAAGGGGCAA+AGG 0.560137 3:+70160744 None:intergenic
CAGGTTTCCGCAGAGTCTGA+AGG 0.567519 3:+70159665 None:intergenic
GATCCTCATTCCTTTCAGCC+CGG 0.568473 3:-70161700 MsaT014267.1:CDS
ATAAACACCACCGGGCTGAA+AGG 0.570819 3:+70161690 None:intergenic
AAGGTATCAGGGTGAAAGAG+TGG 0.579884 3:-70159552 MsaT014267.1:CDS
GTGTTTATTCTGAACCTTGG+TGG 0.581264 3:-70161675 MsaT014267.1:CDS
TCACATACCAACGGCTGGTG+AGG 0.582069 3:+70160920 None:intergenic
AGGTTCAGAATAAACACCAC+CGG 0.582494 3:+70161681 None:intergenic
GGAAGATGATGATGCTGCTA+AGG 0.583652 3:-70161524 MsaT014267.1:CDS
TATTCTGAACCTTGGTGGCG+TGG 0.589853 3:-70161670 MsaT014267.1:CDS
TAACGTCACATACCAACGGC+TGG 0.590772 3:+70160915 None:intergenic
TGCAAAGAAAACTGATGGTG+AGG 0.591393 3:-70159762 MsaT014267.1:CDS
GGATCAAACGAAAATGGTGG+TGG 0.592838 3:-70159611 MsaT014267.1:CDS
ATGCAATCTTCAAGGTATCA+GGG 0.599536 3:-70159563 MsaT014267.1:CDS
GGAAATTACGGACAAGAACA+AGG 0.600249 3:-70161315 MsaT014267.1:CDS
TTATCAGGATCCATATTATG+GGG 0.600718 3:-70160960 MsaT014267.1:CDS
AGGTTCTTGAGCCATCTCAA+GGG 0.605218 3:+70160737 None:intergenic
CCTCATTCCTTTCAGCCCGG+TGG 0.605967 3:-70161697 MsaT014267.1:CDS
CATGCTTTGAGAAGGGCAAG+AGG 0.607747 3:-70159800 MsaT014267.1:CDS
GGTTCTTGAGCCATCTCAAG+GGG 0.609159 3:+70160738 None:intergenic
GTCCAATAGTGAGATGAATG+AGG 0.611764 3:-70161545 MsaT014267.1:CDS
GATTCAGAATCCAATGAGGA+AGG 0.616324 3:-70161577 MsaT014267.1:CDS
ACATCAGCATGCTTTGAGAA+GGG 0.618856 3:-70159807 MsaT014267.1:CDS
GACTGTTCAGGGCAGCTACG+TGG 0.623934 3:-70159521 MsaT014267.1:CDS
TCCTACCATAACAACAACAA+TGG 0.624469 3:-70159587 MsaT014267.1:CDS
CGATTTGCAAAGAAAACTGA+TGG 0.624607 3:-70159767 MsaT014267.1:CDS
CCACCGGGCTGAAAGGAATG+AGG 0.626610 3:+70161697 None:intergenic
GGCAAAGGCATTCTGGCATG+AGG 0.634023 3:+70160759 None:intergenic
AGAAGGGCAAGAGGCACCGG+AGG 0.637214 3:-70159791 MsaT014267.1:CDS
GGGTGAAAGAGTGGAAGAAG+GGG 0.642943 3:-70159543 MsaT014267.1:CDS
CCAGCTGAGGAGTCTGTGTG+AGG 0.649225 3:+70161250 None:intergenic
GGTTCAGAATAAACACCACC+GGG 0.650774 3:+70161682 None:intergenic
AACAATACCAAGGAATTCTG+AGG 0.651910 3:-70160698 MsaT014267.1:CDS
AGAGCCATTATCCTTTCCCG+AGG 0.653284 3:+70159739 None:intergenic
ACCATTGGGACAATCAAGAG+AGG 0.663692 3:+70161600 None:intergenic
TGAGGCACTCCTATAAAAGG+AGG 0.667807 3:+70160777 None:intergenic
TGGAAGCATCTCCTCGAACG+AGG 0.669556 3:-70159501 MsaT014267.1:CDS
AAGGAATGAGGATCTGACCT+CGG 0.671701 3:+70161709 None:intergenic
TGACCAACAAGTTCCAGCTG+AGG 0.675734 3:+70161237 None:intergenic
TATCAGGATCCATATTATGG+GGG 0.690554 3:-70160959 MsaT014267.1:CDS
AGAGGATCAAACGAAAATGG+TGG 0.692972 3:-70159614 MsaT014267.1:CDS
GTGAACGCTAAACAATACCA+AGG 0.697540 3:-70160708 MsaT014267.1:CDS
AACATGCGACAAGATGCAAG+AGG 0.706089 3:-70159632 MsaT014267.1:CDS
TATCCAACACCACGCCACCA+AGG 0.707510 3:+70161661 None:intergenic
TGGTGATTCAGAATCCAATG+AGG 0.715370 3:-70161581 MsaT014267.1:CDS
CCTCGAACGAGGCATCGCAG+AGG 0.718093 3:-70159490 MsaT014267.1:CDS
CCGAAACTGCAAATCAACCG+AGG 0.722272 3:-70161726 MsaT014267.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 70159472 70161761 70159472 ID=MsaG014267
Chr3 mRNA 70159472 70161761 70159472 ID=MsaT014267.1;Parent=MsaG014267
Chr3 exon 70159472 70159849 70159472 ID=MsaT014267.1.exon5;Parent=MsaT014267.1
Chr3 CDS 70159472 70159849 70159472 ID=cds.MsaT014267.1;Parent=MsaT014267.1
Chr3 exon 70160641 70160805 70160641 ID=MsaT014267.1.exon4;Parent=MsaT014267.1
Chr3 CDS 70160641 70160805 70160641 ID=cds.MsaT014267.1;Parent=MsaT014267.1
Chr3 exon 70160928 70160999 70160928 ID=MsaT014267.1.exon3;Parent=MsaT014267.1
Chr3 CDS 70160928 70160999 70160928 ID=cds.MsaT014267.1;Parent=MsaT014267.1
Chr3 exon 70161230 70161336 70161230 ID=MsaT014267.1.exon2;Parent=MsaT014267.1
Chr3 CDS 70161230 70161336 70161230 ID=cds.MsaT014267.1;Parent=MsaT014267.1
Chr3 exon 70161494 70161761 70161494 ID=MsaT014267.1.exon1;Parent=MsaT014267.1
Chr3 CDS 70161494 70161761 70161494 ID=cds.MsaT014267.1;Parent=MsaT014267.1
Gene Sequence

>MsaT014267.1

ATGCAACCCAAGTCCGAAACTGCAAATCAACCGAGGTCAGATCCTCATTCCTTTCAGCCCGGTGGTGTTTATTCTGAACCTTGGTGGCGTGGTGTTGGATACAATCCTGTAGCGCAAACAATGTCTGGTGCCAATTCATCCTCTCTTGATTGTCCCAATGGTGATTCAGAATCCAATGAGGAAGGCCAGTCTTTGTCCAATAGTGAGATGAATGAGGAAGATGATGATGCTGCTAAGGATTCACAGCCTGCTGCTCCCAATCAATCAGGAAATTACGGACAAGAACAAGGAATGCAGAATACTGCATCATCTGCAGTTATTCGCGAAGAATGCCTCACACAGACTCCTCAGCTGGAACTTGTTGGTCATTCAATTGCATGTGCTACAAATCCTTATCAGGATCCATATTATGGGGGCATGATGGCAGCTTATCCTCACCAGCCGTTGGGATATCCTCCTTTTATAGGAGTGCCTCATGCCAGAATGCCTTTGCCCCTTGAGATGGCTCAAGAACCTGTTTATGTGAACGCTAAACAATACCAAGGAATTCTGAGGCGAAGACAAGCTCGTGCTAAAGCAGAGCTTGAAAGGAAGCTCATAAAATCTAGAAAGCCATATCTTCATGAATCTAGACATCAGCATGCTTTGAGAAGGGCAAGAGGCACCGGAGGACGATTTGCAAAGAAAACTGATGGTGAGGCCTCGGGAAAGGATAATGGCTCTGGTCCAGTCTTGTCGTCGCAATCAATTAGTTCATCTGGTTCAGAACTTTTGCCTTCAGACTCTGCGGAAACCTGGAATTCTCCCAACATGCGACAAGATGCAAGAGGATCAAACGAAAATGGTGGTGGCTCCTACCATAACAACAACAATGGTATGCAATCTTCAAGGTATCAGGGTGAAAGAGTGGAAGAAGGGGACTGTTCAGGGCAGCTACGTGGAAGCATCTCCTCGAACGAGGCATCGCAGAGGCGTCTTGCTATTCAGTAG

Protein sequence

>MsaT014267.1

MQPKSETANQPRSDPHSFQPGGVYSEPWWRGVGYNPVAQTMSGANSSSLDCPNGDSESNEEGQSLSNSEMNEEDDDAAKDSQPAAPNQSGNYGQEQGMQNTASSAVIREECLTQTPQLELVGHSIACATNPYQDPYYGGMMAAYPHQPLGYPPFIGVPHARMPLPLEMAQEPVYVNAKQYQGILRRRQARAKAELERKLIKSRKPYLHESRHQHALRRARGTGGRFAKKTDGEASGKDNGSGPVLSSQSISSSGSELLPSDSAETWNSPNMRQDARGSNENGGGSYHNNNNGMQSSRYQGERVEEGDCSGQLRGSISSNEASQRRLAIQ*