AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014330 MtrunA17_Chr3g0104071 98.366 306 3 2 1 305 5 309 0.0 627
MsaG014330 MtrunA17_Chr5g0429521 66.667 303 76 8 1 296 5 289 4.49e-136 388
MsaG014330 MtrunA17_Chr7g0241761 55.263 304 104 9 1 292 11 294 1.32e-102 303
MsaG014330 MtrunA17_Chr5g0436501 64.467 197 56 4 23 217 16 200 7.85e-82 249
MsaG014330 MtrunA17_Chr1g0162081 51.813 193 86 2 31 217 4 195 1.56e-60 195
MsaG014330 MtrunA17_Chr1g0210471 45.528 246 110 7 37 281 22 244 4.82e-59 189
MsaG014330 MtrunA17_Chr4g0062741 46.667 210 75 4 39 217 8 211 4.69e-52 173
MsaG014330 MtrunA17_Chr8g0365011 38.776 196 85 4 36 217 5 179 9.96e-37 133
MsaG014330 MtrunA17_Chr2g0331031 48.322 149 59 5 139 270 104 251 3.86e-33 125
MsaG014330 MtrunA17_Chr4g0055931 60.000 100 38 1 118 217 71 168 4.70e-32 122
MsaG014330 MtrunA17_Chr6g0488471 73.611 72 19 0 138 209 91 162 3.19e-31 118
MsaG014330 MtrunA17_Chr8g0389241 71.233 73 20 1 136 208 97 168 2.81e-30 115
MsaG014330 MtrunA17_Chr5g0415191 68.966 87 25 1 131 217 91 175 3.73e-30 116
MsaG014330 MtrunA17_Chr4g0059701 61.702 94 32 1 132 225 72 161 1.17e-28 112
MsaG014330 MtrunA17_Chr3g0092621 53.846 91 39 1 144 234 84 171 3.10e-25 99.8
MsaG014330 MtrunA17_Chr5g0440731 50.980 102 44 3 114 212 77 175 1.10e-24 101
MsaG014330 MtrunA17_Chr8g0364741 37.778 135 71 2 31 161 2 127 5.39e-24 95.1
MsaG014330 MtrunA17_Chr5g0440741 65.714 70 20 2 146 212 102 170 1.06e-23 98.6
MsaG014330 MtrunA17_Chr3g0135671 58.025 81 27 2 141 214 88 168 1.35e-23 97.1
MsaG014330 MtrunA17_Chr5g0409841 61.333 75 26 2 140 212 88 161 7.88e-23 94.7
MsaG014330 MtrunA17_Chr5g0432921 36.000 175 88 5 31 200 13 168 2.67e-22 93.2
MsaG014330 MtrunA17_Chr5g0432941 34.737 190 91 6 16 200 7 168 9.28e-22 91.7
MsaG014330 MtrunA17_Chr5g0409831 60.526 76 27 1 140 212 73 148 1.25e-20 89.0
MsaG014330 MtrunA17_Chr6g0452791 77.083 48 11 0 144 191 80 127 1.67e-20 87.0
MsaG014330 MtrunA17_Chr3g0144631 56.061 66 29 0 39 104 16 81 5.09e-19 80.1
MsaG014330 MtrunA17_Chr5g0404101 48.529 68 35 0 34 101 14 81 5.83e-19 80.5
MsaG014330 MtrunA17_Chr8g0364731 61.194 67 24 1 153 217 10 76 5.84e-19 83.2
MsaG014330 MtrunA17_Chr1g0155181 51.429 70 34 0 36 105 4 73 9.83e-19 79.7
MsaG014330 MtrunA17_Chr8g0373131 54.412 68 30 1 36 103 2 68 1.39e-18 79.0
MsaG014330 MtrunA17_Chr8g0373111 52.778 72 33 1 36 107 2 72 1.43e-18 78.6
MsaG014330 MtrunA17_Chr8g0373121 52.778 72 33 1 36 107 2 72 1.43e-18 78.6
MsaG014330 MtrunA17_Chr3g0141601 46.753 77 41 0 36 112 4 80 4.44e-18 77.8
MsaG014330 MtrunA17_Chr5g0404301 55.738 61 26 1 39 99 4 63 3.16e-15 69.3
MsaG014330 MtrunA17_Chr6g0449111 47.761 67 35 0 36 102 7 73 3.43e-15 69.7
MsaG014330 MtrunA17_Chr8g0373101 52.174 69 31 2 39 107 5 71 4.42e-15 69.3
MsaG014330 MtrunA17_Chr3g0113121 50.667 75 30 1 146 213 97 171 2.72e-14 70.9
MsaG014330 MtrunA17_Chr3g0081631 49.296 71 35 1 32 101 3 73 5.70e-14 66.6
MsaG014330 MtrunA17_Chr7g0255841 50.769 65 32 0 43 107 16 80 7.21e-14 66.6
MsaG014330 MtrunA17_Chr1g0192061 52.113 71 29 2 146 212 63 132 1.35e-13 68.2
MsaG014330 MtrunA17_Chr8g0370121 43.056 72 36 2 141 208 97 167 9.03e-12 64.3
MsaG014330 MtrunA17_Chr3g0141641 41.667 72 42 0 39 110 3 74 1.74e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014330 AT5G58900.1 69.500 200 52 3 23 215 15 212 5.13e-96 286
MsaG014330 AT2G38090.1 61.883 223 58 4 1 213 5 210 2.59e-94 282
MsaG014330 AT5G05790.2 63.874 191 62 3 35 224 27 211 9.47e-79 241
MsaG014330 AT5G05790.1 63.874 191 62 3 35 224 27 211 9.47e-79 241
MsaG014330 AT3G11280.2 55.000 240 83 6 39 266 33 259 1.17e-77 238
MsaG014330 AT3G11280.1 55.000 240 83 6 39 266 33 259 1.17e-77 238
MsaG014330 AT5G01200.1 57.353 204 67 5 23 213 19 215 1.96e-72 225
MsaG014330 AT5G08520.1 51.075 186 90 1 33 217 6 191 1.20e-57 188
MsaG014330 AT1G49010.1 47.716 197 78 3 39 212 8 202 7.56e-52 174
MsaG014330 AT5G04760.1 49.162 179 79 3 36 214 3 169 1.42e-50 167
MsaG014330 AT5G23650.1 38.866 247 129 5 33 275 7 235 3.52e-42 149
MsaG014330 AT5G47390.1 59.140 93 38 0 124 216 76 168 7.94e-32 122
MsaG014330 AT3G10580.2 35.714 224 109 8 39 250 8 208 5.37e-31 117
MsaG014330 AT3G10580.1 35.714 224 109 8 39 250 8 208 8.12e-31 118
MsaG014330 AT4G09450.1 40.116 172 82 3 38 209 6 156 4.03e-29 111
MsaG014330 AT5G61620.1 54.206 107 45 1 114 220 82 184 5.19e-29 114
MsaG014330 AT5G56840.1 57.609 92 36 2 130 220 79 168 8.48e-28 108
MsaG014330 AT3G16350.1 57.547 106 43 1 120 225 113 216 1.44e-27 111
MsaG014330 AT1G70000.1 68.354 79 23 1 131 209 87 163 3.28e-27 107
MsaG014330 AT1G70000.2 68.354 79 23 1 131 209 87 163 3.28e-27 107
MsaG014330 AT1G74840.2 57.692 104 42 1 139 240 91 194 4.05e-26 104
MsaG014330 AT1G74840.1 57.692 104 42 1 139 240 91 194 6.38e-26 104
MsaG014330 AT1G19000.2 62.791 86 31 1 140 224 98 183 1.89e-25 103
MsaG014330 AT1G19000.1 62.791 86 31 1 140 224 98 183 1.89e-25 103
MsaG014330 AT1G75250.2 53.333 75 35 0 29 103 2 76 2.39e-20 84.7
MsaG014330 AT3G10590.1 37.017 181 98 7 36 209 4 175 4.33e-20 87.0
MsaG014330 AT1G75250.1 53.333 75 35 0 29 103 2 76 4.96e-20 84.7
MsaG014330 AT2G21650.1 54.545 66 30 0 39 104 14 79 2.48e-17 76.6
MsaG014330 AT4G39250.1 46.377 69 37 0 39 107 14 82 8.72e-16 72.4
MsaG014330 AT1G19510.1 54.098 61 28 0 43 103 16 76 7.41e-15 69.7
MsaG014330 AT2G18328.1 50.000 62 31 0 43 104 15 76 1.32e-11 60.1
MsaG014330 AT4G36570.1 61.364 44 17 0 39 82 12 55 6.35e-11 57.8

Find 60 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACGAGTCTTCTTATTT+TGG 0.159828 3:+71173824 MsaT014330.1:CDS
ATTTGACAGGTTATTTCTAT+TGG 0.210653 3:+71173400 MsaT014330.1:intron
GGAGTTAATAATAACTCTTA+TGG 0.241432 3:+71173764 MsaT014330.1:CDS
TATTTCATCAGACAACTTTC+AGG 0.257079 3:+71173518 MsaT014330.1:CDS
TGCTCTTGCAGTTTATGATA+TGG 0.259232 3:+71172576 MsaT014330.1:CDS
AAACCAATTGTTGGTATTGT+TGG 0.291167 3:-71172482 None:intergenic
TTAGATTGGGTGAATTCTTC+TGG 0.292652 3:+71172760 MsaT014330.1:CDS
TTCACCCAATCTAAGGTAAA+AGG 0.308169 3:-71172751 None:intergenic
GCTGGTTTGGTTCCAATTCC+TGG 0.315843 3:+71172706 MsaT014330.1:CDS
GGTGCATGAGGGTGAGAATT+TGG 0.327156 3:-71172445 None:intergenic
CCTTTCTTCCTTTCCTGATC+AGG 0.333627 3:-71172835 None:intergenic
TTTGACAGGTTATTTCTATT+GGG 0.339731 3:+71173401 MsaT014330.1:intron
AGTGGAAAGAGAGGCTCTTC+TGG 0.344816 3:+71172805 MsaT014330.1:CDS
CTCTTGGTTTCTTCTGAAGT+TGG 0.347825 3:-71173614 None:intergenic
ATCTTACTTGTGTTCTTCTT+CGG 0.354438 3:-71172869 None:intergenic
GCATTAACAGGAGCACAAAA+TGG 0.365638 3:+71172524 MsaT014330.1:CDS
AGAAGAATTCACCCAATCTA+AGG 0.374549 3:-71172758 None:intergenic
ACATCAACAACACTCTTTCC+AGG 0.376605 3:-71172637 None:intergenic
TACTTGTTCATAATCATGTT+TGG 0.382661 3:-71173647 None:intergenic
ATTCCAACAATACCAACAAT+TGG 0.395051 3:+71172479 MsaT014330.1:CDS
ATTGTAGCAATAGCATTAAC+AGG 0.398247 3:+71172512 MsaT014330.1:CDS
GATGAATCAGTATAAGGAAT+TGG 0.401455 3:+71172660 MsaT014330.1:CDS
GTAGTACTATAACCAGGAAT+TGG 0.407318 3:-71172718 None:intergenic
GTTTGGTGAAGTTGATCTCT+TGG 0.418126 3:-71173630 None:intergenic
TCAAACAATTTGTTCTCTGC+AGG 0.426400 3:-71172550 None:intergenic
GTGAATTCTTCTGGTTATGA+TGG 0.427738 3:+71172769 MsaT014330.1:CDS
TTGTGTTCTTCTTCGGTCCA+TGG 0.429989 3:-71172862 None:intergenic
GATGTTTGTAATATAGAAGC+TGG 0.431912 3:+71172688 MsaT014330.1:CDS
AAAAGTATGGAAAAGGTGAT+TGG 0.434556 3:+71173429 MsaT014330.1:CDS
TCTGTAACTTCTGATATTGA+AGG 0.437794 3:+71174171 MsaT014330.1:CDS
TTGTAATATAGAAGCTGGTT+TGG 0.450084 3:+71172693 MsaT014330.1:CDS
GAGTTATTATTAACTCCATA+AGG 0.453193 3:-71173758 None:intergenic
TGATGTGATGAATCAGTATA+AGG 0.454237 3:+71172654 MsaT014330.1:CDS
TTCTGGTAGATCTCCTGATC+AGG 0.477520 3:+71172822 MsaT014330.1:CDS
CAAGGAACTTTGTGATCACT+AGG 0.491493 3:+71173462 MsaT014330.1:CDS
TCTTATGGATTTAAAATGCA+AGG 0.530791 3:+71173779 MsaT014330.1:CDS
GCTACCTTATGCCACCGATC+CGG 0.536074 3:-71172604 None:intergenic
AGAGGATTTAGTGGAAAGAG+AGG 0.538143 3:+71172796 MsaT014330.1:CDS
CCTGATCAGGAAAGGAAGAA+AGG 0.540469 3:+71172835 MsaT014330.1:CDS
TTGAATGCTGTCATAGTAGC+TGG 0.544765 3:-71173707 None:intergenic
ATGAAAACTTGTTCATGTGA+AGG 0.546854 3:-71173731 None:intergenic
AAGGTAGCTGCAATGATCCC+TGG 0.550717 3:+71172619 MsaT014330.1:CDS
GCATGGCTAGCTACTTGTGT+TGG 0.551417 3:-71173488 None:intergenic
TACGCCGGATCGGTGGCATA+AGG 0.566659 3:+71172600 MsaT014330.1:CDS
CTCAGAAGTGGTGTTAGCAG+TGG 0.576504 3:-71173672 None:intergenic
TGATGAAATATTTCTGAGCA+TGG 0.587728 3:-71173505 None:intergenic
CTGAAAATGCATCTCAGAAG+TGG 0.589832 3:-71173684 None:intergenic
AAATAAGAAGACTCGTGAAG+AGG 0.594626 3:-71173821 None:intergenic
CATCAACAACACTCTTTCCA+GGG 0.594776 3:-71172636 None:intergenic
GTGATTGGAGAAACATATCA+AGG 0.596845 3:+71173444 MsaT014330.1:CDS
ATGATATGGATACGCCGGAT+CGG 0.596949 3:+71172590 MsaT014330.1:CDS
GTAGATCTCCTGATCAGGAA+AGG 0.600023 3:+71172827 MsaT014330.1:CDS
TTCATCAGACAACTTTCAGG+AGG 0.604647 3:+71173521 MsaT014330.1:CDS
AAAGGAAGAAAGGTGTGCCA+TGG 0.605391 3:+71172845 MsaT014330.1:CDS
ATATGGATACGCCGGATCGG+TGG 0.619279 3:+71172593 MsaT014330.1:CDS
TGGACCTCAGACACAAAACA+TGG 0.622490 3:+71173844 MsaT014330.1:CDS
AGTTTATGATATGGATACGC+CGG 0.628700 3:+71172585 MsaT014330.1:CDS
TATGAAAGGTGAAAGCTGCA+AGG 0.634603 3:+71174139 MsaT014330.1:intron
GCAGCAGTAGTACTATAACC+AGG 0.674867 3:-71172724 None:intergenic
TTTGAACAAATGGTGCATGA+GGG 0.682304 3:-71172456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 71172430 71174200 71172430 ID=MsaG014330
Chr3 mRNA 71172430 71174200 71172430 ID=MsaT014330.1;Parent=MsaG014330
Chr3 exon 71172430 71172884 71172430 ID=MsaT014330.1.exon1;Parent=MsaT014330.1
Chr3 CDS 71172430 71172884 71172430 ID=cds.MsaT014330.1;Parent=MsaT014330.1
Chr3 exon 71173409 71173865 71173409 ID=MsaT014330.1.exon2;Parent=MsaT014330.1
Chr3 CDS 71173409 71173865 71173409 ID=cds.MsaT014330.1;Parent=MsaT014330.1
Chr3 exon 71174147 71174200 71174147 ID=MsaT014330.1.exon3;Parent=MsaT014330.1
Chr3 CDS 71174147 71174200 71174147 ID=cds.MsaT014330.1;Parent=MsaT014330.1
Gene Sequence

>MsaT014330.1

ATGGAAATTCTTTCACCAAATTCTCACCCTCATGCACCATTTGTTCAAAATTCCAACAATACCAACAATTGGTTTTTGGAAGATTGTAGCAATAGCATTAACAGGAGCACAAAATGGACTCCTGCAGAGAACAAATTGTTTGAAAATGCTCTTGCAGTTTATGATATGGATACGCCGGATCGGTGGCATAAGGTAGCTGCAATGATCCCTGGAAAGAGTGTTGTTGATGTGATGAATCAGTATAAGGAATTGGAAGTTGATGTTTGTAATATAGAAGCTGGTTTGGTTCCAATTCCTGGTTATAGTACTACTGCTGCTTCACCTTTTACCTTAGATTGGGTGAATTCTTCTGGTTATGATGGTTTTAGAGGATTTAGTGGAAAGAGAGGCTCTTCTGGTAGATCTCCTGATCAGGAAAGGAAGAAAGGTGTGCCATGGACCGAAGAAGAACACAAGTTATTTCTATTGGGTCTGAAAAAGTATGGAAAAGGTGATTGGAGAAACATATCAAGGAACTTTGTGATCACTAGGACACCAACACAAGTAGCTAGCCATGCTCAGAAATATTTCATCAGACAACTTTCAGGAGGCAAAGACAAAAGAAGAGCTAGCATTCATGACATAACAACAGTTAATCTCACAGAAGTTTCTAGAACTTCTCCAACTTCAGAAGAAACCAAGAGATCAACTTCACCAAACATGATTATGAACAAGTATTCCACTGCTAACACCACTTCTGAGATGCATTTTCAGCCAGCTACTATGACAGCATTCAATCCTTCACATGAACAAGTTTTCATGTCTCCTTATGGAGTTAATAATAACTCTTATGGATTTAAAATGCAAGGTCAAAATATGCATAGAAATCCTCTTCACGAGTCTTCTTATTTTGGACCTCAGACACAAAACATGGTGAAAGCTGCAAGGAAAATATGTTCTGTAACTTCTGATATTGAAGGTAGTTAA
Protein sequence

>MsaT014330.1

MEILSPNSHPHAPFVQNSNNTNNWFLEDCSNSINRSTKWTPAENKLFENALAVYDMDTPDRWHKVAAMIPGKSVVDVMNQYKELEVDVCNIEAGLVPIPGYSTTAASPFTLDWVNSSGYDGFRGFSGKRGSSGRSPDQERKKGVPWTEEEHKLFLLGLKKYGKGDWRNISRNFVITRTPTQVASHAQKYFIRQLSGGKDKRRASIHDITTVNLTEVSRTSPTSEETKRSTSPNMIMNKYSTANTTSEMHFQPATMTAFNPSHEQVFMSPYGVNNNSYGFKMQGQNMHRNPLHESSYFGPQTQNMVKAARKICSVTSDIEGS*