AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014355

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014355 MtrunA17_Chr3g0104431 98.458 389 5 1 1 388 1 389 0.0 794
MsaG014355 MtrunA17_Chr3g0114991 49.798 247 109 10 146 386 212 449 4.39e-64 211
MsaG014355 MtrunA17_Chr1g0156521 45.200 250 111 9 147 388 170 401 4.38e-59 197
MsaG014355 MtrunA17_Chr4g0042091 45.000 240 115 8 153 385 205 434 6.11e-57 192
MsaG014355 MtrunA17_Chr2g0280501 35.849 265 151 6 132 386 120 375 8.05e-44 156
MsaG014355 MtrunA17_Chr8g0376191 41.143 175 91 4 216 388 2 166 4.18e-32 119
MsaG014355 MtrunA17_Chr5g0399041 38.426 216 97 7 175 386 105 288 3.71e-31 120
MsaG014355 MtrunA17_Chr4g0056461 37.089 213 97 7 178 388 98 275 2.89e-30 117
MsaG014355 MtrunA17_Chr5g0445321 31.220 205 112 9 191 387 1 184 2.41e-20 87.8
MsaG014355 MtrunA17_Chr2g0280521 41.071 112 63 2 183 293 11 120 1.36e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014355 AT2G29660.1 55.927 329 120 9 73 388 57 373 1.10e-111 332
MsaG014355 AT5G54630.1 42.164 268 122 9 133 385 220 469 7.60e-58 196
MsaG014355 AT4G27240.1 41.935 279 136 8 115 385 168 428 9.46e-58 194
MsaG014355 AT1G75710.1 42.754 276 114 10 144 388 197 459 5.27e-57 193
MsaG014355 AT4G27240.2 41.270 252 124 7 115 357 168 404 1.32e-48 170
MsaG014355 AT1G11490.2 35.628 247 140 8 150 386 126 363 1.39e-35 134
MsaG014355 AT1G11490.1 35.628 247 140 8 150 386 126 363 1.39e-35 134
MsaG014355 AT4G22560.1 34.440 241 109 10 153 388 68 264 4.44e-28 111
MsaG014355 AT4G12450.1 30.714 280 127 9 118 388 56 277 1.43e-23 99.4
MsaG014355 AT1G62520.1 33.178 214 109 6 176 388 100 280 1.49e-23 99.4

Find 112 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCGAGGAGGTTTGGTTTC+TGG 0.131791 3:-71520299 None:intergenic
ATAAAACTCTTTCTTCTTCT+TGG 0.198943 3:+71520016 MsaT014355.1:CDS
TTTGGGAAGGTGTTTGTTTC+GGG 0.206736 3:-71520411 None:intergenic
GTTTGGGAAGGTGTTTGTTT+CGG 0.289642 3:-71520412 None:intergenic
GGTGAAGAGGGTTGTGATAA+AGG 0.305338 3:-71519970 None:intergenic
TTCAAGATTCGGTGTATGTT+TGG 0.305966 3:-71520429 None:intergenic
ATCCGCAAACCATGCGGTTT+CGG 0.317298 3:+71520329 MsaT014355.1:CDS
ATTTGTGGAATTATTAAATC+AGG 0.319183 3:+71520657 MsaT014355.1:CDS
TGATGACTGGGTGGTGGGAG+TGG 0.330208 3:-71520088 None:intergenic
GTGGAAATTCCATCTAATTT+CGG 0.370485 3:-71520687 None:intergenic
TCGTGAAACGGTGAAGATTA+AGG 0.373459 3:+71520494 MsaT014355.1:CDS
CATGCGGTTTCGGAGCTTAT+CGG 0.386573 3:+71520339 MsaT014355.1:CDS
CACGCAATCCCTTATCTCAT+TGG 0.395281 3:+71520146 MsaT014355.1:CDS
TCAAGATTCGGTGTATGTTT+GGG 0.399126 3:-71520428 None:intergenic
GTTTCCTCCGTGGTGGATCT+TGG 0.402297 3:-71520188 None:intergenic
AGTGCAAATCATCTTCAAAT+CGG 0.403452 3:+71520383 MsaT014355.1:CDS
CGTGGTGGATCTTGGAAACT+CGG 0.414053 3:-71520180 None:intergenic
TGGTCGGGTTGGGTCAGATT+TGG 0.418981 3:+71520815 MsaT014355.1:CDS
TCCGGCAGTCGCGGCGGCGA+CGG 0.422303 3:-71519899 None:intergenic
TGGTGATTCTCGAGGAGGTT+TGG 0.425015 3:-71520306 None:intergenic
CTGAGGTGATGACTGGGTGG+TGG 0.427431 3:-71520094 None:intergenic
GTGTTATCGCTGGTCGGGTT+GGG 0.430878 3:+71520805 MsaT014355.1:CDS
AACTCCCGCTCGATGTCATC+AGG 0.433180 3:-71520744 None:intergenic
TGTTATGGATCTTCAAGATT+CGG 0.443222 3:-71520440 None:intergenic
TGCTTTCACGGTGGTTGTAA+TGG 0.445907 3:-71520121 None:intergenic
CTCCGAAACCGCATGGTTTG+CGG 0.446300 3:-71520331 None:intergenic
ATCTCGCCGCACGCGGTGCA+TGG 0.451668 3:-71520273 None:intergenic
ACCGTCGCCGCCGCGACTGC+CGG 0.453996 3:+71519898 MsaT014355.1:CDS
TATGTGAGTTGGGACATAAC+GGG 0.454446 3:+71520601 MsaT014355.1:CDS
CTCGTTTGTCGTGTTATCGC+TGG 0.455168 3:+71520795 MsaT014355.1:CDS
AAACCGCATGGTTTGCGGAT+TGG 0.458805 3:-71520326 None:intergenic
GGATCTTGGAAACTCGGGTC+GGG 0.459370 3:-71520174 None:intergenic
TTAAGTTCATGAACGTCAAA+CGG 0.460337 3:+71520766 MsaT014355.1:CDS
GGATTGCGTGGATGCTTTCA+CGG 0.461082 3:-71520133 None:intergenic
CGTGTTATCGCTGGTCGGGT+TGG 0.463055 3:+71520804 MsaT014355.1:CDS
TTTCCACGCTGTCGACTAGC+TGG 0.463808 3:+71520703 MsaT014355.1:CDS
TGACTGGGTGGTGGGAGTGG+TGG 0.466592 3:-71520085 None:intergenic
CGGGTTGAACCAATGAGATA+AGG 0.467937 3:-71520155 None:intergenic
AGGGAAGAAGAGGTTGTTAT+CGG 0.474066 3:-71520232 None:intergenic
TAAGTTCATGAACGTCAAAC+GGG 0.478847 3:+71520767 MsaT014355.1:CDS
TGAGGTGATGACTGGGTGGT+GGG 0.484564 3:-71520093 None:intergenic
TGGTGGACTGAGGTGATGAC+TGG 0.491734 3:-71520101 None:intergenic
CACTATGTTGTGACCCGGGT+CGG 0.498754 3:-71520364 None:intergenic
TCTCGCCGCACGCGGTGCAT+GGG 0.499631 3:-71520272 None:intergenic
TCTTTGATGGTAATAATTGA+CGG 0.507796 3:-71519933 None:intergenic
GTGCAAATCATCTTCAAATC+GGG 0.509342 3:+71520384 MsaT014355.1:CDS
TTGTCGTGTTATCGCTGGTC+GGG 0.509981 3:+71520800 MsaT014355.1:CDS
AAGACTTGATGAGGAGGAGT+TGG 0.519786 3:+71520908 MsaT014355.1:CDS
GGGTTGAACCAATGAGATAA+GGG 0.524136 3:-71520154 None:intergenic
TTGATCCTCATTGTCCACCT+TGG 0.524541 3:+71519862 MsaT014355.1:CDS
GGAGCTTATCGGATCCGACC+CGG 0.525050 3:+71520350 MsaT014355.1:CDS
TTAGGGTGAGAAGGTGAAGA+GGG 0.530433 3:-71519982 None:intergenic
TTATGTGAGTTGGGACATAA+CGG 0.530916 3:+71520600 MsaT014355.1:CDS
GACGAACGCTGCATCGCCGA+CGG 0.546210 3:+71520543 MsaT014355.1:CDS
ATCTTCCCATGCACCGCGTG+CGG 0.548197 3:+71520267 MsaT014355.1:CDS
TGGATCTTGGAAACTCGGGT+CGG 0.550081 3:-71520175 None:intergenic
TTTGTCGTGTTATCGCTGGT+CGG 0.550149 3:+71520799 MsaT014355.1:CDS
GGAAGATATCATTGCGGAGA+GGG 0.553280 3:-71520251 None:intergenic
CGGATTGGTGATTCTCGAGG+AGG 0.553761 3:-71520311 None:intergenic
CGGTGAACATGAAGCATCCA+AGG 0.563804 3:-71519879 None:intergenic
TTTGATTCGGTGATGGCGCG+TGG 0.565832 3:+71520867 MsaT014355.1:CDS
ATTTGCACTATGTTGTGACC+CGG 0.567600 3:-71520369 None:intergenic
GTCAGATTTGGACGATGAGG+TGG 0.571954 3:+71520827 MsaT014355.1:CDS
CGATAAGCTCCGAAACCGCA+TGG 0.572249 3:-71520338 None:intergenic
ATTCGAAGAATATCGTGAAA+CGG 0.573251 3:+71520482 MsaT014355.1:CDS
CTGGAAAATCTCGCCGCACG+CGG 0.578813 3:-71520280 None:intergenic
ACGAACGCTGCATCGCCGAC+GGG 0.579320 3:+71520544 MsaT014355.1:CDS
TTTAGGGTGAGAAGGTGAAG+AGG 0.580588 3:-71519983 None:intergenic
TTGCGGATTGGTGATTCTCG+AGG 0.581119 3:-71520314 None:intergenic
TCGGATTGTTTCCTCCGTGG+TGG 0.589003 3:-71520195 None:intergenic
AAGCAAGGAAGAACAGCCCT+AGG 0.591167 3:-71520942 None:intergenic
GTGGTGGATCTTGGAAACTC+GGG 0.591918 3:-71520179 None:intergenic
CAATGAGATAAGGGATTGCG+TGG 0.593615 3:-71520145 None:intergenic
GTGATGGCGCGTGGCGAGAG+TGG 0.595455 3:+71520876 MsaT014355.1:CDS
GACTCGGATTGTTTCCTCCG+TGG 0.596167 3:-71520198 None:intergenic
CCACGCTGTCGACTAGCTGG+AGG 0.602472 3:+71520706 MsaT014355.1:CDS
CACGCTGTCGACTAGCTGGA+GGG 0.602474 3:+71520707 MsaT014355.1:CDS
GATGAGGTGGATAAAACCGA+TGG 0.603948 3:+71520840 MsaT014355.1:CDS
GATTCGGTGTATGTTTGGGA+AGG 0.604252 3:-71520424 None:intergenic
AGCATCCAAGGTGGACAATG+AGG 0.604755 3:-71519867 None:intergenic
CATTGCGGAGAGGGAAGAAG+AGG 0.607506 3:-71520242 None:intergenic
CTATACACAATCACAAAGCA+AGG 0.609481 3:-71520957 None:intergenic
ATGAGGTGGATAAAACCGAT+GGG 0.612087 3:+71520841 MsaT014355.1:CDS
GAGCTTATCGGATCCGACCC+GGG 0.612574 3:+71520351 MsaT014355.1:CDS
AATTCCTGATGACATCGAGC+GGG 0.613181 3:+71520740 MsaT014355.1:CDS
GATATTCTTCGAATTTCGAG+AGG 0.614943 3:-71520473 None:intergenic
GGACTGAGGTGATGACTGGG+TGG 0.616068 3:-71520097 None:intergenic
GGGAAGATATCATTGCGGAG+AGG 0.616437 3:-71520252 None:intergenic
ATGTGAGTTGGGACATAACG+GGG 0.619416 3:+71520602 MsaT014355.1:CDS
AAATCATCTTCAAATCGGGT+TGG 0.627452 3:+71520388 MsaT014355.1:CDS
GGTGGACTGAGGTGATGACT+GGG 0.635403 3:-71520100 None:intergenic
TGTTTACACAAGACTTGATG+AGG 0.640225 3:+71520899 MsaT014355.1:CDS
CGATGCAGCGTTCGTCACGA+CGG 0.642208 3:-71520536 None:intergenic
CAATTCCTGATGACATCGAG+CGG 0.650943 3:+71520739 MsaT014355.1:CDS
ACGCATGAGTTCATTCCCGT+CGG 0.655062 3:-71520559 None:intergenic
TGGGTCAGATTTGGACGATG+AGG 0.659548 3:+71520824 MsaT014355.1:CDS
ATCGGCGGAGATATCTGACT+CGG 0.668831 3:-71520214 None:intergenic
GTGAAGATTAAGGCAGCGCG+TGG 0.670180 3:+71520504 MsaT014355.1:CDS
TGAGGTGGATAAAACCGATG+GGG 0.677373 3:+71520842 MsaT014355.1:CDS
GAAGAAGAGGTTGTTATCGG+CGG 0.680562 3:-71520229 None:intergenic
GAGGTGGATAAAACCGATGG+GGG 0.685349 3:+71520843 MsaT014355.1:CDS
TTGCGTGGATGCTTTCACGG+TGG 0.689558 3:-71520130 None:intergenic
TTTCACGGTGGTTGTAATGG+TGG 0.692003 3:-71520118 None:intergenic
TTTGCACTATGTTGTGACCC+GGG 0.694888 3:-71520368 None:intergenic
TTACACAAGACTTGATGAGG+AGG 0.695990 3:+71520902 MsaT014355.1:CDS
TCACCAATCCGCAAACCATG+CGG 0.707074 3:+71520323 MsaT014355.1:CDS
TGAACATGAAGCATCCAAGG+TGG 0.710959 3:-71519876 None:intergenic
CGAGTTTCCAAGATCCACCA+CGG 0.714229 3:+71520181 MsaT014355.1:CDS
CCTCCAGCTAGTCGACAGCG+TGG 0.733625 3:-71520706 None:intergenic
GTGGTTGTAATGGTGGACTG+AGG 0.746411 3:-71520111 None:intergenic
TGCATGGGAAGATATCATTG+CGG 0.753387 3:-71520257 None:intergenic
GTTTCCAAGATCCACCACGG+AGG 0.776993 3:+71520184 MsaT014355.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 71519820 71520986 71519820 ID=MsaG014355
Chr3 mRNA 71519820 71520986 71519820 ID=MsaT014355.1;Parent=MsaG014355
Chr3 exon 71519820 71520986 71519820 ID=MsaT014355.1.exon1;Parent=MsaT014355.1
Chr3 CDS 71519820 71520986 71519820 ID=cds.MsaT014355.1;Parent=MsaT014355.1
Gene Sequence

>MsaT014355.1

ATGGCTTCTATTGCTACTTTCTTCAACAAATTTTACTATGTTTTGATCCTCATTGTCCACCTTGGATGCTTCATGTTCACCGTCGCCGCCGCGACTGCCGGAAAACAAGATAACCGTCAATTATTACCATCAAAGAAACGAAAAATCTCACCTTTATCACAACCCTCTTCACCTTCTCACCCTAAATTCAAAACCCATAAAACTCTTTCTTCTTCTTGGCATTTCATCAAACACCTCTTCTGCAAAAATTCATGTAAAACTGTAACCACCACTCCCACCACCCAGTCATCACCTCAGTCCACCATTACAACCACCGTGAAAGCATCCACGCAATCCCTTATCTCATTGGTTCAACCCGACCCGAGTTTCCAAGATCCACCACGGAGGAAACAATCCGAGTCAGATATCTCCGCCGATAACAACCTCTTCTTCCCTCTCCGCAATGATATCTTCCCATGCACCGCGTGCGGCGAGATTTTCCAGAAACCAAACCTCCTCGAGAATCACCAATCCGCAAACCATGCGGTTTCGGAGCTTATCGGATCCGACCCGGGTCACAACATAGTGCAAATCATCTTCAAATCGGGTTGGCCCGAAACAAACACCTTCCCAAACATACACCGAATCTTGAAGATCCATAACAGCCAGAAAATCCTCTCGAAATTCGAAGAATATCGTGAAACGGTGAAGATTAAGGCAGCGCGTGGTGTCACGCGCCGTCGTGACGAACGCTGCATCGCCGACGGGAATGAACTCATGCGTTTTCATTGCTCCACTTTTTTATGTGAGTTGGGACATAACGGGGATTCTTCGATTTGTTCTCAGCAATTTTGTAATATTTGTGGAATTATTAAATCAGGATTTTCACCGAAATTAGATGGAATTTCCACGCTGTCGACTAGCTGGAGGGCACATGTGTCAATTCCTGATGACATCGAGCGGGAGTTTAAGTTCATGAACGTCAAACGGGCAATGCTCGTTTGTCGTGTTATCGCTGGTCGGGTTGGGTCAGATTTGGACGATGAGGTGGATAAAACCGATGGGGGTTTTGATTCGGTGATGGCGCGTGGCGAGAGTGGTGTTTACACAAGACTTGATGAGGAGGAGTTGGTTGTTTTTAATCCTAGGGCTGTTCTTCCTTGCTTTGTGATTGTGTATAGTGTGTAA
Protein sequence

>MsaT014355.1

MASIATFFNKFYYVLILIVHLGCFMFTVAAATAGKQDNRQLLPSKKRKISPLSQPSSPSHPKFKTHKTLSSSWHFIKHLFCKNSCKTVTTTPTTQSSPQSTITTTVKASTQSLISLVQPDPSFQDPPRRKQSESDISADNNLFFPLRNDIFPCTACGEIFQKPNLLENHQSANHAVSELIGSDPGHNIVQIIFKSGWPETNTFPNIHRILKIHNSQKILSKFEEYRETVKIKAARGVTRRRDERCIADGNELMRFHCSTFLCELGHNGDSSICSQQFCNICGIIKSGFSPKLDGISTLSTSWRAHVSIPDDIEREFKFMNVKRAMLVCRVIAGRVGSDLDDEVDKTDGGFDSVMARGESGVYTRLDEEELVVFNPRAVLPCFVIVYSV*