AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014418

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014418 MtrunA17_Chr3g0105291 93.009 1130 71 1 3 1132 2 1123 0.0 2151
MsaG014418 MtrunA17_Chr5g0430361 51.166 729 325 12 300 1019 361 1067 0.0 704
MsaG014418 MtrunA17_Chr5g0430361 49.627 268 119 5 23 280 5 266 3.35e-72 261
MsaG014418 MtrunA17_Chr4g0012501 70.400 250 59 4 3 237 2 251 2.39e-109 342
MsaG014418 MtrunA17_Chr5g0407211 41.781 438 218 10 602 1015 1 425 6.08e-109 347
MsaG014418 MtrunA17_Chr5g0407251 41.667 432 215 10 602 1009 1 419 7.32e-108 344
MsaG014418 MtrunA17_Chr7g0270101 43.333 420 217 10 603 1013 383 790 1.30e-105 350
MsaG014418 MtrunA17_Chr3g0134811 37.527 469 216 9 603 1009 338 791 6.36e-97 327
MsaG014418 MtrunA17_Chr3g0134821 40.898 423 219 10 603 1009 425 832 3.87e-93 317
MsaG014418 MtrunA17_Chr1g0205451 39.855 414 236 8 604 1010 606 1013 2.82e-83 298
MsaG014418 MtrunA17_Chr2g0316251 33.136 507 308 9 604 1108 762 1239 2.04e-82 294
MsaG014418 MtrunA17_Chr1g0185491 35.244 471 281 10 550 1010 765 1221 1.37e-79 287
MsaG014418 MtrunA17_Chr3g0134851 34.813 428 182 9 606 1009 110 464 6.50e-58 207
MsaG014418 MtrunA17_Chr7g0259981 32.803 314 185 6 708 1019 13 302 8.00e-56 196
MsaG014418 MtrunA17_Chr7g0265211 27.485 513 282 12 569 1009 453 947 1.24e-43 172
MsaG014418 MtrunA17_Chr1g0182481 27.778 450 261 10 588 992 445 875 5.90e-43 170
MsaG014418 MtrunA17_Chr1g0179841 27.376 442 247 9 626 1007 373 800 5.20e-40 160
MsaG014418 MtrunA17_Chr4g0055731 24.053 528 315 18 499 1009 11 469 5.15e-32 131
MsaG014418 MtrunA17_Chr4g0028111 23.624 436 237 10 636 985 137 562 1.82e-28 122
MsaG014418 MtrunA17_Chr4g0027811 24.138 435 215 10 629 1010 207 579 5.22e-28 120
MsaG014418 MtrunA17_Chr4g0025251 30.651 261 154 9 770 1010 449 702 5.79e-24 108
MsaG014418 MtrunA17_Chr2g0320451 32.804 189 113 4 828 1014 852 1028 2.18e-22 104
MsaG014418 MtrunA17_Chr2g0320451 25.210 238 119 3 629 818 489 715 1.41e-16 85.5
MsaG014418 MtrunA17_Chr3g0101951 30.986 142 92 4 16 153 394 533 1.21e-14 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014418 AT5G58610.3 36.943 1099 562 28 12 1050 23 1050 0.0 673
MsaG014418 AT5G58610.8 37.441 1055 534 25 12 1009 23 1008 0.0 668
MsaG014418 AT5G58610.2 37.441 1055 534 25 12 1009 23 1008 0.0 668
MsaG014418 AT5G58610.5 36.983 1087 554 28 24 1050 26 1041 0.0 666
MsaG014418 AT5G58610.7 36.983 1087 554 28 24 1050 26 1041 0.0 665
MsaG014418 AT5G58610.6 36.431 1087 534 31 24 1050 26 1015 0.0 632
MsaG014418 AT5G58610.1 36.431 1087 534 31 24 1050 26 1015 0.0 632
MsaG014418 AT5G58610.10 36.191 1003 514 25 12 957 23 956 0.0 600
MsaG014418 AT5G58610.9 36.226 991 506 25 24 957 26 947 0.0 592
MsaG014418 AT5G58610.11 35.257 953 491 25 24 919 26 909 4.99e-176 541
MsaG014418 AT5G58610.4 36.184 760 395 17 312 1050 129 819 7.93e-148 465
MsaG014418 AT1G05380.3 38.565 446 261 8 578 1015 494 934 4.71e-90 315
MsaG014418 AT1G05380.1 38.565 446 261 8 578 1015 494 934 4.71e-90 315
MsaG014418 AT1G05380.2 38.565 446 261 8 578 1015 494 934 4.71e-90 315
MsaG014418 AT3G14980.3 35.931 462 278 7 571 1018 575 1032 2.86e-87 305
MsaG014418 AT3G14980.5 35.931 462 278 7 571 1018 530 987 6.01e-87 306
MsaG014418 AT3G14980.6 35.931 462 278 7 571 1018 575 1032 1.02e-86 306
MsaG014418 AT3G14980.1 35.931 462 278 7 571 1018 575 1032 1.02e-86 306
MsaG014418 AT3G14980.2 35.931 462 278 7 571 1018 575 1032 1.02e-86 306
MsaG014418 AT5G36740.5 31.229 602 359 17 454 1019 384 966 1.36e-85 302
MsaG014418 AT5G36740.1 31.229 602 359 17 454 1019 384 966 1.36e-85 302
MsaG014418 AT5G36740.4 28.671 715 422 22 454 1101 231 924 9.62e-82 289
MsaG014418 AT4G14920.3 36.000 450 278 7 577 1022 553 996 3.54e-76 274
MsaG014418 AT4G14920.2 36.384 437 268 7 577 1009 553 983 8.60e-76 273
MsaG014418 AT4G14920.1 36.384 437 268 7 577 1009 553 983 8.60e-76 273
MsaG014418 AT5G36670.4 28.641 618 368 19 454 1019 384 980 2.03e-72 263
MsaG014418 AT5G36670.5 28.641 618 368 19 454 1019 384 980 2.03e-72 263
MsaG014418 AT5G36670.3 26.739 733 427 25 454 1101 231 938 1.18e-68 251
MsaG014418 AT5G36670.2 30.171 527 320 16 454 951 384 891 1.74e-68 248
MsaG014418 AT5G36740.2 30.171 527 320 16 454 951 384 891 1.74e-68 248
MsaG014418 AT5G36740.3 30.171 527 320 16 454 951 384 891 1.74e-68 248
MsaG014418 AT5G36670.1 28.025 628 369 20 454 1019 384 990 2.07e-68 251
MsaG014418 AT5G63900.1 43.322 307 142 11 711 1009 258 540 1.37e-66 235
MsaG014418 AT3G14980.4 33.699 365 224 7 571 921 575 935 1.06e-58 219
MsaG014418 AT2G27980.1 29.425 452 239 10 640 1027 637 1072 3.74e-47 184
MsaG014418 AT2G37520.1 28.935 432 233 10 631 1007 401 813 1.89e-44 174
MsaG014418 AT3G53680.1 28.118 441 248 10 631 1019 418 841 4.89e-43 170
MsaG014418 AT3G53680.2 27.918 437 254 8 631 1019 420 843 5.26e-43 170
MsaG014418 AT2G36720.1 26.974 456 253 10 615 1007 533 971 5.67e-38 155
MsaG014418 AT2G36720.2 26.974 456 253 10 615 1007 533 971 5.67e-38 155

Find 255 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCACCGTACTGTGCTTTC+TGG 0.111625 3:+72800318 MsaT014418.1:CDS
ATTCAGAAATCAACTATTTC+TGG 0.155206 3:+72799976 MsaT014418.1:CDS
AACCAAGACAGAACATTTAA+AGG 0.177176 3:-72800240 None:intergenic
CCAGTTGGCAAAACCGAATT+TGG 0.185947 3:-72805168 None:intergenic
CACATAACTTCCGGATTTAC+TGG 0.222960 3:-72801790 None:intergenic
ACCAAGTGCCAATATCTTTC+TGG 0.242341 3:+72800437 MsaT014418.1:CDS
CGGTATCAGTAAGGCAAATT+TGG 0.243152 3:+72798972 MsaT014418.1:CDS
GAGAATATTCTGAATGATAA+TGG 0.245574 3:+72798593 MsaT014418.1:CDS
TAATAGTAAAAGTGGATTAT+TGG 0.263495 3:+72802145 MsaT014418.1:CDS
CATCAGAAGCTCACGCAATT+AGG 0.270532 3:+72804976 MsaT014418.1:intron
AATTGTGATCTTTCAAAGTT+TGG 0.278158 3:-72805075 None:intergenic
GGGTTTCTATTCCGTACTTC+TGG 0.279523 3:+72804283 MsaT014418.1:CDS
TGAATGGTCAAGGTATTTAA+AGG 0.280119 3:-72800006 None:intergenic
CCGAAAATGGTCTTGGTATA+AGG 0.282380 3:+72799602 MsaT014418.1:CDS
TGAAATGAAGGTCGGAATTC+AGG 0.287717 3:+72798853 MsaT014418.1:CDS
CATCGCTTCGGCGCTTTGGC+AGG 0.290256 3:-72800143 None:intergenic
TGGAAACATCTTGCATATAT+TGG 0.290775 3:+72799445 MsaT014418.1:CDS
ATTTGTTCCGTTTGCCGCTT+TGG 0.297414 3:+72800570 MsaT014418.1:CDS
GATACATGGACCGGCGCTTT+TGG 0.305649 3:+72805039 MsaT014418.1:CDS
TTCATTTCATCTCCCAAATC+AGG 0.306262 3:-72798839 None:intergenic
GCAAGAAGAGGATTCCTTTA+GGG 0.310158 3:-72802225 None:intergenic
AACTCTTCTCCGTCCGAAAA+TGG 0.313482 3:+72799589 MsaT014418.1:CDS
CCTGCAATCGGGCATATAAA+AGG 0.318750 3:+72799794 MsaT014418.1:CDS
GATCTTGCATTATTTGTATC+TGG 0.322209 3:-72800485 None:intergenic
AGTGAGTGTTTCAAGTGTTT+TGG 0.328028 3:+72798637 MsaT014418.1:CDS
GAACCTGTTGTTCCTTCGTC+TGG 0.332759 3:+72799196 MsaT014418.1:CDS
GGGATGAAGTTACAGAGAAA+TGG 0.333962 3:+72798897 MsaT014418.1:CDS
TGAGTAAACGTGGGAGGATT+AGG 0.333992 3:+72798678 MsaT014418.1:CDS
GAGAAATGTGATCTTAAGTA+TGG 0.340078 3:+72798725 MsaT014418.1:CDS
TGCAAGAAGAGGATTCCTTT+AGG 0.345444 3:-72802226 None:intergenic
GCTTGGTAAACTTCAGAAAC+TGG 0.348392 3:-72805330 None:intergenic
TTGTCCGCACCCACTGAAAC+TGG 0.359349 3:-72802071 None:intergenic
CTTATTGCAATGGATTCCAT+TGG 0.361377 3:-72799518 None:intergenic
TTTCTCGGCCTTGAAACTTC+CGG 0.373286 3:+72799123 MsaT014418.1:CDS
AAATATTCCCTCTCCAAATT+CGG 0.374447 3:+72805155 MsaT014418.1:CDS
GAAGCGATGAATCAACTCTA+AGG 0.378506 3:+72800157 MsaT014418.1:CDS
TGGTCAGTGTAGCAACTATT+AGG 0.379578 3:+72804321 MsaT014418.1:CDS
GATGGACTAAGAGGCACATT+TGG 0.383730 3:-72799649 None:intergenic
CATGTTGTTACCAAGGTCTA+AGG 0.385811 3:+72800275 MsaT014418.1:CDS
GTGCGTTGATGTAAACTATC+AGG 0.386742 3:+72798751 MsaT014418.1:CDS
TGTTGTTGGTCCACAGGAAT+TGG 0.390924 3:-72806519 None:intergenic
AATGCAAGATCACAAGTCTA+AGG 0.396731 3:+72800497 MsaT014418.1:CDS
GATTAGGCCAGTGCCTCCTT+TGG 0.397024 3:+72798694 MsaT014418.1:CDS
GAACTTGATAATAGTAAAAG+TGG 0.398807 3:+72802137 MsaT014418.1:CDS
ACCATCGGCCAGAAAGATAT+TGG 0.399541 3:-72800445 None:intergenic
AAACTTCCGGAGATGGAATC+AGG 0.401835 3:+72799136 MsaT014418.1:CDS
TCGCATATTAATGGATGAAT+TGG 0.403985 3:+72804538 MsaT014418.1:CDS
TGGAATGTGTCGCATATTAA+TGG 0.405995 3:+72804529 MsaT014418.1:CDS
ATTACTAGCACTACTGCTTC+CGG 0.409786 3:-72805301 None:intergenic
GGCACCGTACTGTGCTTTCT+GGG 0.413489 3:+72800319 MsaT014418.1:CDS
TTTGTCCATCGTGTCGATGC+GGG 0.414181 3:+72800786 MsaT014418.1:CDS
GTAGCCAAAGCAAATTCAAC+GGG 0.414503 3:+72800813 MsaT014418.1:CDS
ACTTAATCGAATGAATTTCC+AGG 0.417064 3:+72804262 MsaT014418.1:CDS
AAGAGGGGTTTCTGGGATCA+TGG 0.419147 3:+72798519 MsaT014418.1:CDS
TTTCATTCTGGCAATGTTGT+TGG 0.419236 3:-72806533 None:intergenic
TTCTGTCTTGGTTGATAGAC+AGG 0.421112 3:+72800250 MsaT014418.1:CDS
ACAGCCCTTCAAAGTCTGTT+AGG 0.423835 3:+72802044 MsaT014418.1:CDS
TGATGGTACCCGGAAAATCT+AGG 0.425301 3:-72805113 None:intergenic
TTTCTGGGATCATGGCATCC+AGG 0.426138 3:+72798527 MsaT014418.1:CDS
TCATAAGACGTGTCTTGGTC+TGG 0.426619 3:+72800632 MsaT014418.1:CDS
CGAGTATCTCCTCTAGTTGT+GGG 0.428093 3:+72799688 MsaT014418.1:CDS
CCTCCAAATAATAGAAGAAA+AGG 0.429254 3:+72799877 MsaT014418.1:CDS
AACCTTTAAATGTTCTGTCT+TGG 0.434230 3:+72800238 MsaT014418.1:CDS
TTCCATCTCCGGAAGTTTCA+AGG 0.435157 3:-72799131 None:intergenic
CCGGCTTTCCACATAACTTC+CGG 0.435342 3:-72801799 None:intergenic
TCCTTCGAATTTCATGCTAC+TGG 0.436416 3:+72800399 MsaT014418.1:CDS
TTTCTTCTATTATTTGGAGG+TGG 0.441374 3:-72799874 None:intergenic
AGTAGAGATACCAGTAAATC+CGG 0.447861 3:+72801780 MsaT014418.1:CDS
TTATTGCAATGGATTCCATT+GGG 0.450609 3:-72799517 None:intergenic
CTCTTCTTGCAGAGATCTGA+TGG 0.450854 3:+72802238 MsaT014418.1:CDS
TCTTAGGGTCCTTGGAGATA+AGG 0.451187 3:+72804460 MsaT014418.1:intron
TTTGTGGAGACACTTGATAA+TGG 0.452655 3:+72799060 MsaT014418.1:CDS
GTTCACACTGAATGCATGTA+AGG 0.456612 3:-72800856 None:intergenic
TCTTCTTGAGTGCATGTTTG+TGG 0.458079 3:-72799771 None:intergenic
GGACAAAACCATTCACCATC+AGG 0.458765 3:-72800770 None:intergenic
AATGGGAGCGACGAGGGAAA+TGG 0.459932 3:+72798915 MsaT014418.1:CDS
TCATGAGGAAAATGTTGATC+AGG 0.461489 3:+72800026 MsaT014418.1:CDS
CACTCAAGAAGACCTGCAAT+CGG 0.463785 3:+72799782 MsaT014418.1:CDS
TGAATGATAAGAAGGATTTG+TGG 0.464023 3:+72799044 MsaT014418.1:CDS
AGGTGAACTATTATCTGAAA+AGG 0.467810 3:+72799156 MsaT014418.1:CDS
TTAGAGTTGATTCATCGCTT+CGG 0.468082 3:-72800155 None:intergenic
GATTCATCGCTTCGGCGCTT+TGG 0.468963 3:-72800147 None:intergenic
GGCCTTGAAACTTCCGGAGA+TGG 0.471082 3:+72799129 MsaT014418.1:CDS
AGGCAAGAGGAAGACGCCTT+TGG 0.471117 3:-72800204 None:intergenic
CAAATTTGCCTTACTGATAC+CGG 0.471321 3:-72798971 None:intergenic
GATTGAGGAGTCGTATCTTC+CGG 0.473378 3:+72798952 MsaT014418.1:CDS
AGAATATTCTGAATGATAAT+GGG 0.475569 3:+72798594 MsaT014418.1:CDS
TTTCCAGACGAAGGAACAAC+AGG 0.475742 3:-72799199 None:intergenic
ATCGTTGGATCAACAAATGA+TGG 0.477086 3:+72805374 MsaT014418.1:CDS
TGGCAATGTTGTTGGTCCAC+AGG 0.477715 3:-72806525 None:intergenic
GGATGAAGTTACAGAGAAAT+GGG 0.477755 3:+72798898 MsaT014418.1:CDS
AATACGGATTACTCAGGATT+GGG 0.481439 3:+72798877 MsaT014418.1:CDS
AACATCTTGCATATATTGGA+TGG 0.482403 3:+72799449 MsaT014418.1:CDS
GGCAACTATGACATCTTTGA+AGG 0.483635 3:+72799414 MsaT014418.1:CDS
TCTGGAGAGAAATGAAGATC+TGG 0.483681 3:+72804301 MsaT014418.1:CDS
GATAGACAAAAGAGAATCGT+TGG 0.484937 3:+72805359 MsaT014418.1:CDS
AAAAGTGCTCTCTTGTAAAG+TGG 0.486293 3:+72806586 MsaT014418.1:CDS
ACATATTGCTTAACAGCATC+GGG 0.489875 3:-72799373 None:intergenic
AGTAAACTTTCTTATTGCAA+TGG 0.489917 3:-72799528 None:intergenic
CGAATAAGTCAAGTGCAAAT+CGG 0.490132 3:+72800088 MsaT014418.1:CDS
ATGACGACGATACTGAAATC+TGG 0.490750 3:-72804507 None:intergenic
AACTCAACCAAAGGAGGCAC+TGG 0.491829 3:-72798701 None:intergenic
ATTCTGAATGATAATGGGTT+GGG 0.491960 3:+72798599 MsaT014418.1:CDS
AATTTGCCGCGATTGGTGAA+TGG 0.492343 3:+72799017 MsaT014418.1:CDS
AGTTCACCTGATTCCATCTC+CGG 0.494011 3:-72799142 None:intergenic
TCTCCGTCCGAAAATGGTCT+TGG 0.497129 3:+72799595 MsaT014418.1:CDS
CAGTAAATCCGGAAGTTATG+TGG 0.497239 3:+72801791 MsaT014418.1:CDS
AAATACGGATTACTCAGGAT+TGG 0.497965 3:+72798876 MsaT014418.1:CDS
TTCGCCTCCAAAGCGGCAAA+CGG 0.498884 3:-72800577 None:intergenic
TTCATTTCTCTCCAGAAGTA+CGG 0.498951 3:-72804294 None:intergenic
GAAGGCGATAATGATGATCT+TGG 0.500051 3:+72806199 MsaT014418.1:CDS
AGGTCGGAATTCAGGAAATA+CGG 0.503071 3:+72798861 MsaT014418.1:CDS
TATTCTGAATGATAATGGGT+TGG 0.503416 3:+72798598 MsaT014418.1:CDS
TGTTGTTCCTTCGTCTGGAA+AGG 0.504783 3:+72799201 MsaT014418.1:CDS
GAGAAGAATTTCTGTTTCAG+TGG 0.506628 3:-72799916 None:intergenic
ATGGACATTGAATGATAAGA+AGG 0.507839 3:+72799036 MsaT014418.1:CDS
GTAGCTGAAGTACCTCTTGT+TGG 0.510042 3:+72804482 MsaT014418.1:CDS
TTAAAGGTTTATGATGTGAA+AGG 0.510336 3:-72800224 None:intergenic
TCACATTCAATAACTATCCC+TGG 0.512351 3:-72798545 None:intergenic
GATATTGGCACTTGGTCTGA+TGG 0.512828 3:-72800430 None:intergenic
GCTGTCCTAACAGACTTTGA+AGG 0.513374 3:-72802049 None:intergenic
GCCAATATCTTTCTGGCCGA+TGG 0.514917 3:+72800444 MsaT014418.1:CDS
CATCAGATCTCTGCAAGAAG+AGG 0.515594 3:-72802237 None:intergenic
CACATATTGCTTAACAGCAT+CGG 0.516322 3:-72799374 None:intergenic
CGAAAACAAGATGCTATCTC+CGG 0.516683 3:+72805282 MsaT014418.1:CDS
GTTAGGACAGCCAGTTTCAG+TGG 0.517338 3:+72802061 MsaT014418.1:CDS
ACCAGGGTGGTAAACAATAC+AGG 0.518289 3:+72799485 MsaT014418.1:CDS
GGAGGTAGTTGGTGACTACT+TGG 0.518838 3:+72799081 MsaT014418.1:CDS
CGTATCTTCCGGTATCAGTA+AGG 0.521329 3:+72798963 MsaT014418.1:CDS
GGTACTGAGATCAAATGAAA+GGG 0.522206 3:+72800179 MsaT014418.1:CDS
TGTTCCGTTTGCCGCTTTGG+AGG 0.523366 3:+72800573 MsaT014418.1:CDS
AGGATCTCCCTGATGGTGAA+TGG 0.525582 3:+72800762 MsaT014418.1:intron
CCGAGTATCTCCTCTAGTTG+TGG 0.526543 3:+72799687 MsaT014418.1:CDS
ATCTGTTCAGATGGACTAAG+AGG 0.527881 3:-72799658 None:intergenic
GGACGGAGAAGAGTTCATCA+TGG 0.529822 3:-72799581 None:intergenic
TTCTGGGATCATGGCATCCA+GGG 0.530488 3:+72798528 MsaT014418.1:CDS
TGAATGTTTCGAGCCCCTAA+AGG 0.530764 3:+72802211 MsaT014418.1:CDS
CTTAATCGAATGAATTTCCA+GGG 0.533875 3:+72804263 MsaT014418.1:CDS
CAAACTGAAGTTTCATGCAA+CGG 0.534310 3:+72799238 MsaT014418.1:CDS
GGGTACTGAGATCAAATGAA+AGG 0.534729 3:+72800178 MsaT014418.1:CDS
AAACTATCAGGAAGCTTGGT+GGG 0.534916 3:+72798763 MsaT014418.1:CDS
CTAGACGAAGGAAGGCGAAA+GGG 0.537831 3:+72806625 MsaT014418.1:CDS
GACAATATTACATGTTCCCA+CGG 0.538718 3:-72799334 None:intergenic
ATCATCTGAAAGCAGAAGGA+TGG 0.539714 3:+72799839 MsaT014418.1:CDS
TTGGGAGATGAAATGAAGGT+CGG 0.540759 3:+72798845 MsaT014418.1:CDS
CTACTTGGCTGTTACAGTAA+AGG 0.541628 3:+72799096 MsaT014418.1:CDS
TATCAGGAAGCTTGGTGGGA+AGG 0.543603 3:+72798767 MsaT014418.1:CDS
TCTTGTAAAGTGGAGTGGAC+TGG 0.545279 3:+72806596 MsaT014418.1:CDS
ATCAAATGAAAGGGTGCCAA+AGG 0.545984 3:+72800188 MsaT014418.1:CDS
CCGGAAGTTATGTGGAAAGC+CGG 0.546646 3:+72801799 MsaT014418.1:CDS
CCAAACCCTATCAATGGAGG+AGG 0.547670 3:+72798054 None:intergenic
ACCTGTATTGTTTACCACCC+TGG 0.547980 3:-72799486 None:intergenic
TCTACCACAGTGGGAACAGC+AGG 0.548324 3:-72805018 None:intergenic
CGGTTCTGAATCTGTTCAGA+TGG 0.549079 3:-72799667 None:intergenic
TTCCCACTGTGGTAGATACA+TGG 0.549468 3:+72805025 MsaT014418.1:CDS
ATGTAAACTATCAGGAAGCT+TGG 0.554158 3:+72798759 MsaT014418.1:CDS
CATGTATAGGATCTCCCTGA+TGG 0.557404 3:+72800755 MsaT014418.1:intron
TGCTCTCTTGTAAAGTGGAG+TGG 0.557520 3:+72806591 MsaT014418.1:CDS
AGATTTCAGTATCGTCGTCA+TGG 0.558925 3:+72804509 MsaT014418.1:CDS
TTCTGGCACATGATGGTACC+CGG 0.559347 3:-72805123 None:intergenic
AAATGATACGTGTGATGGAA+TGG 0.561531 3:+72798796 MsaT014418.1:CDS
TCATATCATAAGACGTGTCT+TGG 0.562434 3:+72800627 MsaT014418.1:CDS
ATCGGAAGCGAAAACGCTTG+AGG 0.563012 3:+72800106 MsaT014418.1:CDS
CGCAAGTCCTTTCCAGACGA+AGG 0.564340 3:-72799208 None:intergenic
ACTCAAGAAGACCTGCAATC+GGG 0.566161 3:+72799783 MsaT014418.1:CDS
CAAGAAGAGGATTCCTTTAG+GGG 0.566868 3:-72802224 None:intergenic
ATGGGAAATTGACATTCACC+AGG 0.568448 3:+72799468 MsaT014418.1:CDS
CTGCGGAAAAGACTGTGAAA+AGG 0.570141 3:+72801824 MsaT014418.1:CDS
CTGTCCTAACAGACTTTGAA+GGG 0.570641 3:-72802048 None:intergenic
TGGTATTCCACAATGGCTTG+AGG 0.570911 3:-72799751 None:intergenic
CAGAGAAATGGGAGCGACGA+GGG 0.570951 3:+72798909 MsaT014418.1:CDS
ATTTAAAGTCAAATAATCCG+TGG 0.572355 3:+72799317 MsaT014418.1:CDS
TTGCAATGGATTCCATTGGG+TGG 0.572732 3:-72799514 None:intergenic
TCATCTGCCACTGCAACAGA+TGG 0.573346 3:-72799715 None:intergenic
TTAGGACAGCCAGTTTCAGT+GGG 0.574149 3:+72802062 MsaT014418.1:CDS
TCATTTCATCTCCCAAATCA+GGG 0.574454 3:-72798838 None:intergenic
GGAACATGACAGTCACCGGC+AGG 0.574813 3:-72799628 None:intergenic
TCAGGAAATACGGATTACTC+AGG 0.576247 3:+72798871 MsaT014418.1:CDS
ACATCTTGCATATATTGGAT+GGG 0.577259 3:+72799450 MsaT014418.1:CDS
GAAGTTTCATGCAACGGTGC+TGG 0.578059 3:+72799244 MsaT014418.1:CDS
TAAACTATCAGGAAGCTTGG+TGG 0.578343 3:+72798762 MsaT014418.1:CDS
ATTAATGGATGAATTGGAGA+AGG 0.579634 3:+72804544 MsaT014418.1:CDS
AGAAATCATCTGAAAGCAGA+AGG 0.585694 3:+72799835 MsaT014418.1:CDS
GCCAGTAGCATGAAATTCGA+AGG 0.587705 3:-72800400 None:intergenic
AAATACCTTGACCATTCATG+AGG 0.588685 3:+72800011 MsaT014418.1:CDS
GGCAAAACCGAATTTGGAGA+GGG 0.589581 3:-72805162 None:intergenic
ACGTCTTATGATATGAAGAC+GGG 0.594381 3:-72800620 None:intergenic
ACAGCCAGTTTCAGTGGGTG+CGG 0.594757 3:+72802067 MsaT014418.1:CDS
ATTTGGAACATGACAGTCAC+CGG 0.595709 3:-72799632 None:intergenic
GCCAGAAAGATATTGGCACT+TGG 0.596693 3:-72800438 None:intergenic
TCTGGGCGAATAACTCATGA+AGG 0.597089 3:+72800336 MsaT014418.1:CDS
TGTAGCCAAAGCAAATTCAA+CGG 0.597213 3:+72800812 MsaT014418.1:CDS
CTTTGAAAATCCAAAAGCGC+CGG 0.597314 3:-72805049 None:intergenic
TGATTTGGGAGATGAAATGA+AGG 0.601531 3:+72798841 MsaT014418.1:CDS
GAAAATCAATGAAAGAGTTG+AGG 0.603862 3:+72798111 MsaT014418.1:CDS
TACAGGTACAAGCCACCCAA+TGG 0.603909 3:+72799502 MsaT014418.1:CDS
CACGTCTTATGATATGAAGA+CGG 0.605602 3:-72800621 None:intergenic
CACTTGATAATGGAGGTAGT+TGG 0.609618 3:+72799070 MsaT014418.1:CDS
GGTGTACTCTAGACGAAGGA+AGG 0.610377 3:+72806617 MsaT014418.1:CDS
CAATGTCCATTCACCAATCG+CGG 0.611041 3:-72799023 None:intergenic
TTCTCAAACTCAACCAAAGG+AGG 0.613689 3:-72798707 None:intergenic
GGGGCTTCCATCTGTTGCAG+TGG 0.616057 3:+72799708 MsaT014418.1:CDS
TAAGACGTGTCTTGGTCTGG+AGG 0.616442 3:+72800635 MsaT014418.1:CDS
TATGTGGAAAGCCGGTTCTG+CGG 0.616945 3:+72801807 MsaT014418.1:CDS
TAGACGAAGGAAGGCGAAAG+GGG 0.617367 3:+72806626 MsaT014418.1:CDS
GAAAGGGGAACATGAAGCGT+AGG 0.617607 3:+72806641 MsaT014418.1:CDS
GATGGAAGCCCCACAACTAG+AGG 0.618341 3:-72799697 None:intergenic
CTTGGTATAAGGCATCCTGC+CGG 0.619801 3:+72799613 MsaT014418.1:CDS
AAGCGATGAATCAACTCTAA+GGG 0.620220 3:+72800158 MsaT014418.1:CDS
TTTATGATGTGAAAGGCAAG+AGG 0.620240 3:-72800217 None:intergenic
TTCGCCCAGAAAGCACAGTA+CGG 0.622172 3:-72800323 None:intergenic
AGATAAGAGCGATCTACCAT+CGG 0.623826 3:-72800460 None:intergenic
ATGTTTGTGGTATTCCACAA+TGG 0.624693 3:-72799758 None:intergenic
TCTAGACGAAGGAAGGCGAA+AGG 0.625198 3:+72806624 MsaT014418.1:CDS
ATCGGGTCATTGAGTAAACG+TGG 0.626423 3:+72798668 MsaT014418.1:CDS
GGCGGAGCAACCAATTCCTG+TGG 0.627539 3:+72806509 MsaT014418.1:intron
TTTAAAGTCAAATAATCCGT+GGG 0.628929 3:+72799318 MsaT014418.1:CDS
TGAAATCTGGTACCAACAAG+AGG 0.629514 3:-72804494 None:intergenic
TGGCAAAACCGAATTTGGAG+AGG 0.630456 3:-72805163 None:intergenic
GTTGTAAATGATACGTGTGA+TGG 0.630651 3:+72798791 MsaT014418.1:CDS
CCACACAGTCATTTGCGTCA+AGG 0.632292 3:+72800534 MsaT014418.1:CDS
ACTGTGGTAGATACATGGAC+CGG 0.633569 3:+72805030 MsaT014418.1:CDS
ATGCAACGGTGCTGGTGAAG+CGG 0.634047 3:+72799252 MsaT014418.1:CDS
CCACAACTAGAGGAGATACT+CGG 0.637983 3:-72799687 None:intergenic
AAACGCTTGAGGAATTCAGA+TGG 0.640084 3:+72800117 MsaT014418.1:CDS
GAAGTACGGAATAGAAACCC+TGG 0.641639 3:-72804280 None:intergenic
CTTCAGCTACCTTATCTCCA+AGG 0.642106 3:-72804469 None:intergenic
AAGTTCACAACTCTCAGAGT+CGG 0.642483 3:-72802120 None:intergenic
TTATTGTCCTCAAGCCATTG+TGG 0.643240 3:+72799744 MsaT014418.1:CDS
CGTGTGATGGAATGGAGGAG+AGG 0.644455 3:+72798804 MsaT014418.1:CDS
GTGGAGACACTTGATAATGG+AGG 0.645022 3:+72799063 MsaT014418.1:CDS
CATTTCTCAAACTCAACCAA+AGG 0.645043 3:-72798710 None:intergenic
GAAGCTCACGCAATTAGGAG+TGG 0.645058 3:+72804981 MsaT014418.1:intron
GCAAGATCACAAGTCTAAGG+AGG 0.646340 3:+72800500 MsaT014418.1:CDS
TGATACGTGTGATGGAATGG+AGG 0.646808 3:+72798799 MsaT014418.1:CDS
GAGTATCTCCTCTAGTTGTG+GGG 0.652442 3:+72799689 MsaT014418.1:CDS
ACAGGAACATGTTGTTACCA+AGG 0.652952 3:+72800268 MsaT014418.1:CDS
GTCCATGTATCTACCACAGT+GGG 0.654636 3:-72805027 None:intergenic
CAAATCCCGCATCGACACGA+TGG 0.658482 3:-72800791 None:intergenic
GACAAAACCATTCACCATCA+GGG 0.668378 3:-72800769 None:intergenic
ACAGAGAAATGGGAGCGACG+AGG 0.673479 3:+72798908 MsaT014418.1:CDS
TTTACCTGCTGTTCCCACTG+TGG 0.676231 3:+72805014 MsaT014418.1:CDS
TGGGTGCGGACAATCTAACA+TGG 0.678752 3:+72802081 MsaT014418.1:CDS
AAAGAAGTTCGCCTCCAAAG+CGG 0.687855 3:-72800584 None:intergenic
CCTTGACGCAAATGACTGTG+TGG 0.688106 3:-72800534 None:intergenic
CAAACCCTATCAATGGAGGA+GGG 0.690346 3:+72798055 None:intergenic
GGTCATTGAGTAAACGTGGG+AGG 0.693475 3:+72798672 MsaT014418.1:CDS
TGTTATCGACTGTGTCACCA+TGG 0.695665 3:+72806228 MsaT014418.1:CDS
TCGGGTCATTGAGTAAACGT+GGG 0.701785 3:+72798669 MsaT014418.1:CDS
GACTGGTGTACTCTAGACGA+AGG 0.706505 3:+72806613 MsaT014418.1:CDS
TGGGAAATTGACATTCACCA+GGG 0.734667 3:+72799469 MsaT014418.1:CDS
AGCCAAAGCAAATTCAACGG+GGG 0.735187 3:+72800815 MsaT014418.1:CDS
TAGCCAAAGCAAATTCAACG+GGG 0.739394 3:+72800814 MsaT014418.1:CDS
GGTCCATGTATCTACCACAG+TGG 0.749610 3:-72805028 None:intergenic
GAAATTGACATTCACCAGGG+TGG 0.761755 3:+72799472 MsaT014418.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 72798067 72806662 72798067 ID=MsaG014418
Chr3 mRNA 72798067 72806662 72798067 ID=MsaT014418.1;Parent=MsaG014418
Chr3 exon 72798067 72798132 72798067 ID=MsaT014418.1.exon1;Parent=MsaT014418.1
Chr3 CDS 72798067 72798132 72798067 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72798506 72800656 72798506 ID=MsaT014418.1.exon2;Parent=MsaT014418.1
Chr3 CDS 72798506 72800656 72798506 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72800764 72800884 72800764 ID=MsaT014418.1.exon3;Parent=MsaT014418.1
Chr3 CDS 72800764 72800884 72800764 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72801760 72801845 72801760 ID=MsaT014418.1.exon4;Parent=MsaT014418.1
Chr3 CDS 72801760 72801845 72801760 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72802038 72802282 72802038 ID=MsaT014418.1.exon5;Parent=MsaT014418.1
Chr3 CDS 72802038 72802282 72802038 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72804256 72804342 72804256 ID=MsaT014418.1.exon6;Parent=MsaT014418.1
Chr3 CDS 72804256 72804342 72804256 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72804466 72804565 72804466 ID=MsaT014418.1.exon7;Parent=MsaT014418.1
Chr3 CDS 72804466 72804565 72804466 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72804982 72805189 72804982 ID=MsaT014418.1.exon8;Parent=MsaT014418.1
Chr3 CDS 72804982 72805189 72804982 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72805274 72805397 72805274 ID=MsaT014418.1.exon9;Parent=MsaT014418.1
Chr3 CDS 72805274 72805397 72805274 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72806192 72806249 72806192 ID=MsaT014418.1.exon10;Parent=MsaT014418.1
Chr3 CDS 72806192 72806249 72806192 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Chr3 exon 72806510 72806662 72806510 ID=MsaT014418.1.exon11;Parent=MsaT014418.1
Chr3 CDS 72806510 72806662 72806510 ID=cds.MsaT014418.1;Parent=MsaT014418.1
Gene Sequence

>MsaT014418.1

ATGGAGGAGGGTTTCGAGACTTCATCAAACTCGCAATCAAAGCTGAAAATCAATGAAAGAGTTGAGGTGAAGAGTTTTGAAGAGGGGTTTCTGGGATCATGGCATCCAGGGATAGTTATTGAATGTGAGAAGCTGAAGCGTCATGTTCGATATGAGAATATTCTGAATGATAATGGGTTGGGTAATTTTGTGGAGGTAGTGAGTGTTTCAAGTGTTTTGGATGGTGATATCGGGTCATTGAGTAAACGTGGGAGGATTAGGCCAGTGCCTCCTTTGGTTGAGTTTGAGAAATGTGATCTTAAGTATGGTTTGTGCGTTGATGTAAACTATCAGGAAGCTTGGTGGGAAGGTGTTGTAAATGATACGTGTGATGGAATGGAGGAGAGGACTGTGTTTTTCCCTGATTTGGGAGATGAAATGAAGGTCGGAATTCAGGAAATACGGATTACTCAGGATTGGGATGAAGTTACAGAGAAATGGGAGCGACGAGGGAAATGGTTGCTTTTTGAATTGATTGAGGAGTCGTATCTTCCGGTATCAGTAAGGCAAATTTGGTATGATGTGCGACAAAAAAAGGAATTTGCCGCGATTGGTGAATGGACATTGAATGATAAGAAGGATTTGTGGAGACACTTGATAATGGAGGTAGTTGGTGACTACTTGGCTGTTACAGTAAAGGAAGTTTTCTCGGCCTTGAAACTTCCGGAGATGGAATCAGGTGAACTATTATCTGAAAAGGAAAAGTTTGAGCAGAAAGAACCTGTTGTTCCTTCGTCTGGAAAGGACTTGCGTAAGTTTCAAACTGAAGTTTCATGCAACGGTGCTGGTGAAGCGGTTGCCATCAATTTTGAGAAAAAAGAACACAGATTGTCAACTGATTTAAAGTCAAATAATCCGTGGGAACATGTAATATTGTCTGAAACTGAATACTGTCCCGATGCTGTTAAGCAATATGTGCTTGCTTTTGACGGCAAGGCAACTATGACATCTTTGAAGGAAAAAGTATGGAAACATCTTGCATATATTGGATGGGAAATTGACATTCACCAGGGTGGTAAACAATACAGGTACAAGCCACCCAATGGAATCCATTGCAATAAGAAAGTTTACTACTCACTTATTGAACTTTGTAAAGCTGAATCCATGATGAACTCTTCTCCGTCCGAAAATGGTCTTGGTATAAGGCATCCTGCCGGTGACTGTCATGTTCCAAATGTGCCTCTTAGTCCATCTGAACAGATTCAGAACCGAGTATCTCCTCTAGTTGTGGGGCTTCCATCTGTTGCAGTGGCAGATGAACAAGCTTATTGTCCTCAAGCCATTGTGGAATACCACAAACATGCACTCAAGAAGACCTGCAATCGGGCATATAAAAGGAAACTTATTTCAAAAGTAAGAAATCATCTGAAAGCAGAAGGATGGATTTTAATTGATCCACCTCCAAATAATAGAAGAAAAGGTGTTTTATACATTTCTCCACTGAAACAGAAATTCTTCTCATTGAATACAGTATGCAGTTTCTTACTTGAAGAAAGTATTCAGAAATCAACTATTTCTGGCATGCAACCTTTAAATACCTTGACCATTCATGAGGAAAATGTTGATCAGGTATTGAGTGATGATTTATCAAGTAGAGACAGTGTTGCTGCGAATAAGTCAAGTGCAAATCGGAAGCGAAAACGCTTGAGGAATTCAGATGGCAGCCTGCCAAAGCGCCGAAGCGATGAATCAACTCTAAGGGTACTGAGATCAAATGAAAGGGTGCCAAAGGCGTCTTCCTCTTGCCTTTCACATCATAAACCTTTAAATGTTCTGTCTTGGTTGATAGACAGGAACATGTTGTTACCAAGGTCTAAGGTTTTTTGTCGAGCAAAACGTGGGCACCGTACTGTGCTTTCTGGGCGAATAACTCATGAAGGCATCAAGTGTAACTGTTGTTCAAGAATATACAGTCTTGTTTCCTTCGAATTTCATGCTACTGGCAGTAATACCATCAGACCAAGTGCCAATATCTTTCTGGCCGATGGTAGATCGCTCTTATCTTGCCAGATACAAATAATGCAAGATCACAAGTCTAAGGAGGCTATGATAAAACCACACAGTCATTTGCGTCAAGGTGAAAACGACTACATTTGTTCCGTTTGCCGCTTTGGAGGCGAACTTCTTTTGTGTGATCAATGCCCGTCTTCATATCATAAGACGTGTCTTGGTCTGGAGGATCTCCCTGATGGTGAATGGTTTTGTCCATCGTGTCGATGCGGGATTTGTAGCCAAAGCAAATTCAACGGGGGTGGTGAGGATGAGCATTTCCTTACATGCATTCAGTGTGAACATAAATATCATGTTGCGTGCCTGAAAAGTAGAGATACCAGTAAATCCGGAAGTTATGTGGAAAGCCGGTTCTGCGGAAAAGACTGTGAAAAGTTATATACAGCCCTTCAAAGTCTGTTAGGACAGCCAGTTTCAGTGGGTGCGGACAATCTAACATGGACATTAGTGAAGTGTGCCGACTCTGAGAGTTGTGAACTTGATAATAGTAAAAGTGGATTATTGGCAGAAAGTTACAGCAAACTCAATGTTGCTCTTTCATTGATGCATGAATGTTTCGAGCCCCTAAAGGAATCCTCTTCTTGCAGAGATCTGATGGAAAATGTTTTATTCAGCCGATGGTCAGAACTTAATCGAATGAATTTCCAGGGTTTCTATTCCGTACTTCTGGAGAGAAATGAAGATCTGGTCAGTGTAGCAACTATTAGGGTCCTTGGAGATAAGGTAGCTGAAGTACCTCTTGTTGGTACCAGATTTCAGTATCGTCGTCATGGAATGTGTCGCATATTAATGGATGAATTGGAGAAGAAGCTCACGCAATTAGGAGTGGAACGTCTCGTTTTACCTGCTGTTCCCACTGTGGTAGATACATGGACCGGCGCTTTTGGATTTTCAAAGATGCCAAACTTTGAAAGATCACAATTTCTAAACTATACTTTCCTAGATTTTCCGGGTACCATCATGTGCCAGAAGTTTTTAGCAAATATTCCCTCTCCAAATTCGGTTTTGCCAACTGAGTTCCAACGAAAACAAGATGCTATCTCCGGAAGCAGTAGTGCTAGTAATAAGTCTCCAGTTTCTGAAGTTTACCAAGCAGAAGAGATAGACAAAAGAGAATCGTTGGATCAACAAATGATGGATACTTCTGAAGGCGATAATGATGATCTTGGCAGTGCTGTTATCGACTGTGTCACCATGGCGGAGCAACCAATTCCTGTGGACCAACAACATTGCCAGAATGAAACCACTCAAAAATGCCAGAATGAAACCACTCAAAAGTGCTCTCTTGTAAAGTGGAGTGGACTGGTGTACTCTAGACGAAGGAAGGCGAAAGGGGAACATGAAGCGTAG
Protein sequence

>MsaT014418.1

MEEGFETSSNSQSKLKINERVEVKSFEEGFLGSWHPGIVIECEKLKRHVRYENILNDNGLGNFVEVVSVSSVLDGDIGSLSKRGRIRPVPPLVEFEKCDLKYGLCVDVNYQEAWWEGVVNDTCDGMEERTVFFPDLGDEMKVGIQEIRITQDWDEVTEKWERRGKWLLFELIEESYLPVSVRQIWYDVRQKKEFAAIGEWTLNDKKDLWRHLIMEVVGDYLAVTVKEVFSALKLPEMESGELLSEKEKFEQKEPVVPSSGKDLRKFQTEVSCNGAGEAVAINFEKKEHRLSTDLKSNNPWEHVILSETEYCPDAVKQYVLAFDGKATMTSLKEKVWKHLAYIGWEIDIHQGGKQYRYKPPNGIHCNKKVYYSLIELCKAESMMNSSPSENGLGIRHPAGDCHVPNVPLSPSEQIQNRVSPLVVGLPSVAVADEQAYCPQAIVEYHKHALKKTCNRAYKRKLISKVRNHLKAEGWILIDPPPNNRRKGVLYISPLKQKFFSLNTVCSFLLEESIQKSTISGMQPLNTLTIHEENVDQVLSDDLSSRDSVAANKSSANRKRKRLRNSDGSLPKRRSDESTLRVLRSNERVPKASSSCLSHHKPLNVLSWLIDRNMLLPRSKVFCRAKRGHRTVLSGRITHEGIKCNCCSRIYSLVSFEFHATGSNTIRPSANIFLADGRSLLSCQIQIMQDHKSKEAMIKPHSHLRQGENDYICSVCRFGGELLLCDQCPSSYHKTCLGLEDLPDGEWFCPSCRCGICSQSKFNGGGEDEHFLTCIQCEHKYHVACLKSRDTSKSGSYVESRFCGKDCEKLYTALQSLLGQPVSVGADNLTWTLVKCADSESCELDNSKSGLLAESYSKLNVALSLMHECFEPLKESSSCRDLMENVLFSRWSELNRMNFQGFYSVLLERNEDLVSVATIRVLGDKVAEVPLVGTRFQYRRHGMCRILMDELEKKLTQLGVERLVLPAVPTVVDTWTGAFGFSKMPNFERSQFLNYTFLDFPGTIMCQKFLANIPSPNSVLPTEFQRKQDAISGSSSASNKSPVSEVYQAEEIDKRESLDQQMMDTSEGDNDDLGSAVIDCVTMAEQPIPVDQQHCQNETTQKCQNETTQKCSLVKWSGLVYSRRRKAKGEHEA*