AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG014420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014420 MtrunA17_Chr3g0105291 83.234 841 77 9 182 975 300 1123 0.0 1359
MsaG014420 MtrunA17_Chr3g0105291 76.444 225 8 2 3 182 2 226 2.53e-100 340
MsaG014420 MtrunA17_Chr5g0430361 46.685 739 295 14 186 862 366 1067 0.0 620
MsaG014420 MtrunA17_Chr5g0430361 40.465 215 72 4 23 182 5 218 9.97e-40 159
MsaG014420 MtrunA17_Chr5g0407211 40.225 445 220 13 438 858 3 425 2.70e-101 323
MsaG014420 MtrunA17_Chr5g0407251 40.091 439 217 13 438 852 3 419 3.71e-100 321
MsaG014420 MtrunA17_Chr7g0270101 41.725 429 220 13 437 856 383 790 7.67e-99 328
MsaG014420 MtrunA17_Chr3g0134811 36.192 478 219 12 437 852 338 791 2.68e-89 303
MsaG014420 MtrunA17_Chr3g0134821 39.583 432 221 13 437 852 425 832 6.99e-88 300
MsaG014420 MtrunA17_Chr1g0205451 39.007 423 236 10 438 853 606 1013 3.08e-79 283
MsaG014420 MtrunA17_Chr2g0316251 33.138 513 305 12 360 852 672 1166 1.21e-78 280
MsaG014420 MtrunA17_Chr1g0185491 34.216 453 272 11 411 853 785 1221 3.56e-73 265
MsaG014420 MtrunA17_Chr4g0012501 53.750 240 51 5 3 182 2 241 8.05e-67 224
MsaG014420 MtrunA17_Chr7g0259981 31.988 322 186 8 542 862 13 302 1.10e-50 181
MsaG014420 MtrunA17_Chr7g0265211 27.011 522 282 13 403 852 453 947 7.33e-41 163
MsaG014420 MtrunA17_Chr1g0182481 27.233 459 261 11 422 835 445 875 1.41e-40 162
MsaG014420 MtrunA17_Chr1g0179841 26.608 451 248 10 460 850 373 800 1.63e-35 145
MsaG014420 MtrunA17_Chr3g0134851 37.262 263 121 8 440 678 110 352 1.50e-29 123
MsaG014420 MtrunA17_Chr3g0134851 45.763 118 64 0 735 852 347 464 3.29e-28 119
MsaG014420 MtrunA17_Chr4g0055731 26.852 432 236 17 433 852 106 469 1.46e-28 120
MsaG014420 MtrunA17_Chr4g0028111 23.583 441 240 9 470 828 137 562 3.03e-26 115
MsaG014420 MtrunA17_Chr4g0027811 23.198 444 217 12 463 853 207 579 6.59e-21 98.2
MsaG014420 MtrunA17_Chr4g0025251 29.630 270 154 10 604 853 449 702 2.73e-20 96.7
MsaG014420 MtrunA17_Chr2g0320451 29.798 198 116 5 662 857 852 1028 9.57e-18 89.0
MsaG014420 MtrunA17_Chr2g0320451 25.210 238 119 3 463 652 489 715 2.26e-16 84.3
MsaG014420 MtrunA17_Chr3g0101951 29.730 148 95 5 16 156 394 539 7.17e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG014420 AT5G58610.4 43.976 498 261 7 403 893 333 819 7.99e-133 421
MsaG014420 AT5G58610.3 43.976 498 261 7 403 893 564 1050 1.33e-130 422
MsaG014420 AT5G58610.3 37.333 225 86 6 12 182 23 246 5.13e-35 145
MsaG014420 AT5G58610.5 43.976 498 261 7 403 893 555 1041 1.80e-130 421
MsaG014420 AT5G58610.5 37.559 213 78 6 24 182 26 237 7.67e-33 138
MsaG014420 AT5G58610.7 43.976 498 261 7 403 893 555 1041 3.03e-130 421
MsaG014420 AT5G58610.7 37.559 213 78 6 24 182 26 237 9.09e-33 137
MsaG014420 AT5G58610.8 46.374 455 229 5 403 852 564 1008 4.74e-130 418
MsaG014420 AT5G58610.8 37.333 225 86 6 12 182 23 246 5.52e-35 145
MsaG014420 AT5G58610.2 46.374 455 229 5 403 852 564 1008 4.74e-130 418
MsaG014420 AT5G58610.2 37.333 225 86 6 12 182 23 246 5.52e-35 145
MsaG014420 AT5G58610.6 42.771 498 241 10 403 893 555 1015 4.09e-118 387
MsaG014420 AT5G58610.6 37.559 213 78 6 24 182 26 237 6.01e-33 138
MsaG014420 AT5G58610.1 42.771 498 241 10 403 893 555 1015 4.85e-118 387
MsaG014420 AT5G58610.1 37.559 213 78 6 24 182 26 237 6.53e-33 138
MsaG014420 AT5G58610.9 44.417 403 209 5 403 800 555 947 3.68e-105 350
MsaG014420 AT5G58610.9 37.559 213 78 6 24 182 26 237 6.76e-33 138
MsaG014420 AT5G58610.10 44.417 403 209 5 403 800 564 956 4.01e-105 350
MsaG014420 AT5G58610.10 37.333 225 86 6 12 182 23 246 4.41e-35 145
MsaG014420 AT5G58610.11 42.740 365 194 5 403 762 555 909 4.24e-86 296
MsaG014420 AT5G58610.11 37.559 213 78 6 24 182 26 237 8.33e-33 137
MsaG014420 AT1G05380.3 36.923 455 265 10 412 858 494 934 7.36e-84 294
MsaG014420 AT1G05380.1 36.923 455 265 10 412 858 494 934 7.36e-84 294
MsaG014420 AT1G05380.2 36.923 455 265 10 412 858 494 934 7.36e-84 294
MsaG014420 AT3G14980.3 35.244 471 278 9 405 861 575 1032 1.72e-83 292
MsaG014420 AT3G14980.5 35.244 471 278 9 405 861 530 987 2.36e-83 293
MsaG014420 AT3G14980.6 35.244 471 278 9 405 861 575 1032 3.62e-83 293
MsaG014420 AT3G14980.1 35.244 471 278 9 405 861 575 1032 3.62e-83 293
MsaG014420 AT3G14980.2 35.244 471 278 9 405 861 575 1032 3.62e-83 293
MsaG014420 AT5G36740.5 33.578 545 302 17 438 944 545 1067 8.61e-80 283
MsaG014420 AT5G36740.1 33.578 545 302 17 438 944 545 1067 8.61e-80 283
MsaG014420 AT5G36740.4 32.793 555 303 18 438 944 392 924 3.37e-76 271
MsaG014420 AT4G14920.3 34.792 457 283 8 411 865 553 996 3.66e-71 257
MsaG014420 AT4G14920.2 35.135 444 273 8 411 852 553 983 9.10e-71 256
MsaG014420 AT4G14920.1 35.135 444 273 8 411 852 553 983 9.10e-71 256
MsaG014420 AT5G36670.4 30.660 561 311 19 438 944 545 1081 1.05e-66 244
MsaG014420 AT5G36670.5 30.660 561 311 19 438 944 545 1081 1.05e-66 244
MsaG014420 AT5G36670.3 29.947 571 312 20 438 944 392 938 9.07e-63 231
MsaG014420 AT5G36670.1 29.947 571 312 20 438 944 545 1091 1.26e-62 232
MsaG014420 AT5G36670.2 35.792 366 207 12 438 794 545 891 5.33e-62 228
MsaG014420 AT5G36740.2 35.792 366 207 12 438 794 545 891 5.33e-62 228
MsaG014420 AT5G36740.3 35.792 366 207 12 438 794 545 891 5.33e-62 228
MsaG014420 AT5G63900.1 39.938 323 153 12 545 859 258 547 8.02e-62 221
MsaG014420 AT3G14980.4 32.888 374 224 9 405 764 575 935 1.71e-54 206
MsaG014420 AT2G27980.1 28.172 465 237 12 474 870 637 1072 1.26e-42 169
MsaG014420 AT2G37520.1 28.118 441 234 10 465 850 401 813 1.28e-41 165
MsaG014420 AT3G53680.1 27.130 446 255 8 465 862 418 841 4.00e-40 160
MsaG014420 AT3G53680.2 27.130 446 255 8 465 862 420 843 4.07e-40 160
MsaG014420 AT2G36720.1 26.882 465 251 11 449 850 533 971 9.49e-36 147
MsaG014420 AT2G36720.2 26.882 465 251 11 449 850 533 971 9.49e-36 147

Find 215 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCACCGTACTGTGCTTTC+TGG 0.111625 3:+72839786 MsaT014420.1:CDS
AAACACCTTCTTCTATTATT+TGG 0.117583 3:-72839357 None:intergenic
ATTCAGAAATCGACTATTTC+TGG 0.151862 3:+72839451 MsaT014420.1:CDS
CCAGTTGGCAAAACCGAATT+TGG 0.185947 3:-72843901 None:intergenic
ACCAAGTGCCAATATCTTTC+TGG 0.242341 3:+72839905 MsaT014420.1:CDS
CGGTATCAGTAAGGCAAATT+TGG 0.243152 3:+72838460 MsaT014420.1:CDS
GAGAATATTCTGAATGATAA+TGG 0.245574 3:+72838090 MsaT014420.1:CDS
AACATAACTTCCGGATTTAC+TGG 0.249735 3:-72840540 None:intergenic
TAATAGTAAAAGTGGATTAT+TGG 0.263495 3:+72840894 MsaT014420.1:CDS
CATCAGAAGCTCACGCAATT+AGG 0.270532 3:+72843709 MsaT014420.1:intron
AATTGTGATCTTTCAAAGTT+TGG 0.278158 3:-72843808 None:intergenic
GGGTTTCTATTCCGTACTTC+TGG 0.279523 3:+72843015 MsaT014420.1:CDS
TGAATGGTCAAGGTATTTAA+AGG 0.280119 3:-72839481 None:intergenic
CCGAAAATGGTCTTGGTATA+AGG 0.282380 3:+72839080 MsaT014420.1:CDS
CATCGCTTCGGCGCTTTGGC+AGG 0.290256 3:-72839611 None:intergenic
TCCTTTAACTGCCCGATTGC+AGG 0.293056 3:-72839272 None:intergenic
ATTTGTTCCGTTTGCCGCTT+TGG 0.297414 3:+72840038 MsaT014420.1:CDS
GATACATGGACCGGCGCTTT+TGG 0.305649 3:+72843772 MsaT014420.1:CDS
AACTCTTCTCCGTCCGAAAA+TGG 0.313482 3:+72839067 MsaT014420.1:CDS
GCAAGAAGAGGATTCCTTTA+GGG 0.319265 3:-72840972 None:intergenic
GATCTTGCATTATTTGTATC+TGG 0.322209 3:-72839953 None:intergenic
AGTGAGTGTTTCAAGTGTTT+TGG 0.328028 3:+72838134 MsaT014420.1:CDS
TGAGTAAACGTGGGAGGATT+AGG 0.333992 3:+72838175 MsaT014420.1:CDS
GAGAAATGTGATCTTAAGTA+TGG 0.340078 3:+72838222 MsaT014420.1:CDS
TGCAAGAAGAGGATTCCTTT+AGG 0.345444 3:-72840973 None:intergenic
GCTTGGTAAACTTCAGAAAC+TGG 0.348392 3:-72844063 None:intergenic
TTGTCCGCACCCACTGAAAC+TGG 0.359349 3:-72840820 None:intergenic
CTTATTGCAATGGATTCCAT+TGG 0.361377 3:-72838996 None:intergenic
CATGTTGTTACCAAGGTCTA+AGG 0.373440 3:+72839743 MsaT014420.1:CDS
AAATATTCCCTCTCCAAATT+CGG 0.374447 3:+72843888 MsaT014420.1:CDS
GAAGCGATGAATCAACTCTA+AGG 0.378506 3:+72839625 MsaT014420.1:CDS
TGGTCAGTGTAGCAACTATT+AGG 0.379578 3:+72843053 MsaT014420.1:CDS
GATGGACTAAGAGGCACATT+TGG 0.383730 3:-72839127 None:intergenic
GTGCGTTGATGTAAACTATC+AGG 0.386742 3:+72838248 MsaT014420.1:CDS
AAGCAATATGTGCTTGCTTT+TGG 0.393038 3:+72838868 MsaT014420.1:CDS
AATGCAAGATCACAAGTCTA+AGG 0.396731 3:+72839965 MsaT014420.1:CDS
GATTAGGCCAGTGCCTCCTT+TGG 0.397024 3:+72838191 MsaT014420.1:CDS
GAACTTGATAATAGTAAAAG+TGG 0.398807 3:+72840886 MsaT014420.1:CDS
ACCATCGGCCAGAAAGATAT+TGG 0.399541 3:-72839913 None:intergenic
TCGCATATTAATGGATGAAT+TGG 0.403985 3:+72843271 MsaT014420.1:CDS
TGGAATGTGTCGCATATTAA+TGG 0.405995 3:+72843262 MsaT014420.1:CDS
TGTTGTTGGTCCACATGAAT+TGG 0.411126 3:-72845250 None:intergenic
AACCAAGACAGAACATTTGA+AGG 0.413342 3:-72839708 None:intergenic
GGCACCGTACTGTGCTTTCT+GGG 0.413489 3:+72839787 MsaT014420.1:CDS
TTTGTCCATCGTGTCGATGC+GGG 0.414181 3:+72840254 MsaT014420.1:CDS
GTAGCCAAAGCAAATTCAAC+GGG 0.414503 3:+72840281 MsaT014420.1:CDS
ACTTAATCGAATGAATTTCC+AGG 0.417064 3:+72842994 MsaT014420.1:CDS
TATCCATCAAAATATGTTCA+TGG 0.417408 3:+72841042 MsaT014420.1:CDS
AAGAGGGGTTTCTGGGATCA+TGG 0.419147 3:+72838016 MsaT014420.1:CDS
TTTCATTCTGGCAATGTTGT+TGG 0.419236 3:-72845264 None:intergenic
ACAGCCCTTCAAAGTCTGTT+AGG 0.423835 3:+72840793 MsaT014420.1:CDS
TGATGGTACCCGGAAAATCT+AGG 0.425301 3:-72843846 None:intergenic
TTTCTGGGATCATGGCATCC+AGG 0.426138 3:+72838024 MsaT014420.1:CDS
TCATAAGACGTGTCTTGGTC+TGG 0.426619 3:+72840100 MsaT014420.1:CDS
TCCTTCGAATTTCATGCTAC+TGG 0.436416 3:+72839867 MsaT014420.1:CDS
CACCTTCTTCTATTATTTGG+AGG 0.440291 3:-72839354 None:intergenic
ATTACTAGCAGTACTGCTTC+CGG 0.441845 3:-72844034 None:intergenic
TCTTAGGGTCCTTGGAGATA+AGG 0.443751 3:+72843193 MsaT014420.1:intron
CCGGCTTTCAACATAACTTC+CGG 0.444864 3:-72840549 None:intergenic
AGTAGAGATACCAGTAAATC+CGG 0.447861 3:+72840530 MsaT014420.1:CDS
AACCTTCAAATGTTCTGTCT+TGG 0.449908 3:+72839706 MsaT014420.1:CDS
TTATTGCAATGGATTCCATT+GGG 0.450609 3:-72838995 None:intergenic
CTCTTCTTGCAGAGATCTGA+TGG 0.450854 3:+72840985 MsaT014420.1:CDS
TTTGTGGAGACACTTGATAA+TGG 0.452655 3:+72838545 MsaT014420.1:CDS
GTTCACACTGAATGCATGTA+AGG 0.456612 3:-72840324 None:intergenic
TCTTCTTGAGTGCATGTTTG+TGG 0.458079 3:-72839249 None:intergenic
GGACAAAACCATTCACCATC+AGG 0.458765 3:-72840238 None:intergenic
CAAGTATCTCCTCTAGTTGT+GGG 0.458939 3:+72839166 MsaT014420.1:CDS
TCATGAGGAAAATGTTGATC+AGG 0.461489 3:+72839501 MsaT014420.1:CDS
CACTCAAGAAGACCTGCAAT+CGG 0.463785 3:+72839260 MsaT014420.1:CDS
TGAATGATAAGAAGGATTTG+TGG 0.464023 3:+72838529 MsaT014420.1:CDS
TTAGAGTTGATTCATCGCTT+CGG 0.468082 3:-72839623 None:intergenic
GATTCATCGCTTCGGCGCTT+TGG 0.468963 3:-72839615 None:intergenic
CATGAATGTTTGAGCCCTAA+AGG 0.469475 3:+72840958 MsaT014420.1:CDS
AGGCAAGAGGAAGACGCCTT+TGG 0.471117 3:-72839672 None:intergenic
CAAATTTGCCTTACTGATAC+CGG 0.471321 3:-72838459 None:intergenic
GAAGGCGATAATGATAATCT+TGG 0.474333 3:+72844930 MsaT014420.1:CDS
AGAATATTCTGAATGATAAT+GGG 0.475569 3:+72838091 MsaT014420.1:CDS
ATCGTTGGATCAACAAATGA+TGG 0.477086 3:+72844107 MsaT014420.1:CDS
CTTCTTCTATTATTTGGAGG+TGG 0.479071 3:-72839351 None:intergenic
GGCAACTATGACATCTTTGA+AGG 0.483635 3:+72838894 MsaT014420.1:CDS
TCTGGAGAGAAATGAAGATC+TGG 0.483681 3:+72843033 MsaT014420.1:CDS
GATAGACAAAAGAGAATCGT+TGG 0.484937 3:+72844092 MsaT014420.1:CDS
TGATTGAGAGTCGTATCTTC+CGG 0.485057 3:+72838440 MsaT014420.1:intron
AAAAGTGCTCTCTTGTAAAG+TGG 0.486293 3:+72845317 MsaT014420.1:CDS
ACATATTGCTTAACAGCATC+GGG 0.489875 3:-72838856 None:intergenic
AGTAAACTTTCTTATTGCAA+TGG 0.489917 3:-72839006 None:intergenic
ATGACGACGATACTGAAATC+TGG 0.490750 3:-72843240 None:intergenic
AACTCAACCAAAGGAGGCAC+TGG 0.491829 3:-72838198 None:intergenic
ATTCTGAATGATAATGGGTT+GGG 0.491960 3:+72838096 MsaT014420.1:CDS
TCTCCGTCCGAAAATGGTCT+TGG 0.497129 3:+72839073 MsaT014420.1:CDS
TTCGCCTCCAAAGCGGCAAA+CGG 0.498884 3:-72840045 None:intergenic
TTCATTTCTCTCCAGAAGTA+CGG 0.498951 3:-72843026 None:intergenic
TATTCTGAATGATAATGGGT+TGG 0.503416 3:+72838095 MsaT014420.1:CDS
GAGAAGAATTTCTGTTTCAG+TGG 0.506628 3:-72839391 None:intergenic
ATGGACATTGAATGATAAGA+AGG 0.507839 3:+72838521 MsaT014420.1:CDS
TGGTTCTGAATCTGTTCAGA+TGG 0.509501 3:-72839145 None:intergenic
TCACTATCAATAACTGTCCC+TGG 0.509655 3:-72838042 None:intergenic
GTAGCTGAAGTACCTCTTGT+TGG 0.510042 3:+72843215 MsaT014420.1:CDS
GATATTGGCACTTGGTCTGA+TGG 0.512828 3:-72839898 None:intergenic
GCTGTCCTAACAGACTTTGA+AGG 0.513374 3:-72840798 None:intergenic
GCCAATATCTTTCTGGCCGA+TGG 0.514917 3:+72839912 MsaT014420.1:CDS
CATCAGATCTCTGCAAGAAG+AGG 0.515594 3:-72840984 None:intergenic
TTGAAGGTTTATGATGTGAA+AGG 0.515956 3:-72839692 None:intergenic
CACATATTGCTTAACAGCAT+CGG 0.516322 3:-72838857 None:intergenic
CGAAAACAAGATGCTATCTC+CGG 0.516683 3:+72844015 MsaT014420.1:CDS
GTTAGGACAGCCAGTTTCAG+TGG 0.517338 3:+72840810 MsaT014420.1:CDS
ACCAGGGTGGTAAACAATAC+AGG 0.518289 3:+72838963 MsaT014420.1:intron
GGAGGTAGTTGGTGACTACT+TGG 0.518838 3:+72838566 MsaT014420.1:CDS
TTCTGGGATCATGGCATCCA+GGG 0.519201 3:+72838025 MsaT014420.1:CDS
CCAAGTATCTCCTCTAGTTG+TGG 0.519696 3:+72839165 MsaT014420.1:CDS
GGAATTACGCGATTGGTGAA+TGG 0.520102 3:+72838502 MsaT014420.1:CDS
AGTGCAAATCAGGCAGCGGC+AGG 0.520490 3:+72839574 MsaT014420.1:intron
CGTATCTTCCGGTATCAGTA+AGG 0.521329 3:+72838451 MsaT014420.1:CDS
GGTACTGAGATCAAATGAAA+GGG 0.522206 3:+72839647 MsaT014420.1:CDS
TGTTCCGTTTGCCGCTTTGG+AGG 0.523366 3:+72840041 MsaT014420.1:CDS
AGGATCTCCCTGATGGTGAA+TGG 0.525582 3:+72840230 MsaT014420.1:intron
ATCTGTTCAGATGGACTAAG+AGG 0.527881 3:-72839136 None:intergenic
GGACGGAGAAGAGTTCATCA+TGG 0.529822 3:-72839059 None:intergenic
CCACAACTAGAGGAGATACT+TGG 0.533352 3:-72839165 None:intergenic
CTTAATCGAATGAATTTCCA+GGG 0.533875 3:+72842995 MsaT014420.1:CDS
GGGTACTGAGATCAAATGAA+AGG 0.534729 3:+72839646 MsaT014420.1:CDS
AAACTATCAGGAAGCTTGGT+GGG 0.534916 3:+72838260 MsaT014420.1:CDS
CTAGACGAAGGAAGGCGAAA+GGG 0.537831 3:+72845356 MsaT014420.1:CDS
CACCTCCAAATAATAGAAGA+AGG 0.539682 3:+72839352 MsaT014420.1:CDS
CTACTTGGCTGTTACAGTAA+AGG 0.541628 3:+72838581 MsaT014420.1:CDS
TATCAGGAAGCTTGGTGGGA+AGG 0.543603 3:+72838264 MsaT014420.1:CDS
CCGGAAGTTATGTTGAAAGC+CGG 0.544451 3:+72840549 MsaT014420.1:CDS
TCTTGTAAAGTGGAGTGGAC+TGG 0.545279 3:+72845327 MsaT014420.1:CDS
ATCAAATGAAAGGGTGCCAA+AGG 0.545984 3:+72839656 MsaT014420.1:CDS
CCAAACCCTATCAATGGAGG+AGG 0.547670 3:+72837544 None:intergenic
TCTACCACAGTGGGAACAGC+AGG 0.548324 3:-72843751 None:intergenic
TTCCCACTGTGGTAGATACA+TGG 0.549468 3:+72843758 MsaT014420.1:CDS
ATGTAAACTATCAGGAAGCT+TGG 0.554158 3:+72838256 MsaT014420.1:CDS
CATGTATAGGATCTCCCTGA+TGG 0.557404 3:+72840223 MsaT014420.1:intron
TGCTCTCTTGTAAAGTGGAG+TGG 0.557520 3:+72845322 MsaT014420.1:CDS
AGATTTCAGTATCGTCGTCA+TGG 0.558925 3:+72843242 MsaT014420.1:CDS
TTCTGGCACATGATGGTACC+CGG 0.559347 3:-72843856 None:intergenic
AAATGATACGTGTGATGGAA+TGG 0.561531 3:+72838293 MsaT014420.1:CDS
TCATATCATAAGACGTGTCT+TGG 0.562434 3:+72840095 MsaT014420.1:CDS
ACTCAAGAAGACCTGCAATC+GGG 0.566161 3:+72839261 MsaT014420.1:CDS
CTGCGGAAAAGACTGTGAAA+AGG 0.570141 3:+72840574 MsaT014420.1:CDS
CTGTCCTAACAGACTTTGAA+GGG 0.570641 3:-72840797 None:intergenic
TGGTATTCCACAATGGCTTG+AGG 0.570911 3:-72839229 None:intergenic
TTGCAATGGATTCCATTGGG+TGG 0.572732 3:-72838992 None:intergenic
TCATCTGCCACTGCAACAGA+TGG 0.573346 3:-72839193 None:intergenic
TTAGGACAGCCAGTTTCAGT+GGG 0.574149 3:+72840811 MsaT014420.1:CDS
GGAACATGACAGTCACCGGC+AGG 0.574813 3:-72839106 None:intergenic
TAAACTATCAGGAAGCTTGG+TGG 0.578343 3:+72838259 MsaT014420.1:CDS
ATTAATGGATGAATTGGAGA+AGG 0.579634 3:+72843277 MsaT014420.1:CDS
GCCAGTAGCATGAAATTCGA+AGG 0.587705 3:-72839868 None:intergenic
AAATACCTTGACCATTCATG+AGG 0.588685 3:+72839486 MsaT014420.1:CDS
GGCAAAACCGAATTTGGAGA+GGG 0.589581 3:-72843895 None:intergenic
ACGTCTTATGATATGAAGAC+GGG 0.594381 3:-72840088 None:intergenic
ACAGCCAGTTTCAGTGGGTG+CGG 0.594757 3:+72840816 MsaT014420.1:CDS
ATTTGGAACATGACAGTCAC+CGG 0.595709 3:-72839110 None:intergenic
GCCAGAAAGATATTGGCACT+TGG 0.596693 3:-72839906 None:intergenic
TCTGGGCGAATAACTCATGA+AGG 0.597089 3:+72839804 MsaT014420.1:CDS
TGTAGCCAAAGCAAATTCAA+CGG 0.597213 3:+72840280 MsaT014420.1:CDS
CTTTGAAAATCCAAAAGCGC+CGG 0.597314 3:-72843782 None:intergenic
TATGTTGAAAGCCGGTTCTG+CGG 0.597913 3:+72840557 MsaT014420.1:CDS
GGATATGAAACTCTTACCAT+CGG 0.600453 3:-72841024 None:intergenic
GAAAATCAATGAAAGAGTTG+AGG 0.603862 3:+72837601 MsaT014420.1:CDS
TACAGGTACAAGCCACCCAA+TGG 0.603909 3:+72838980 MsaT014420.1:CDS
CACGTCTTATGATATGAAGA+CGG 0.605602 3:-72840089 None:intergenic
CACTTGATAATGGAGGTAGT+TGG 0.609618 3:+72838555 MsaT014420.1:CDS
GGTGTACTCTAGACGAAGGA+AGG 0.610377 3:+72845348 MsaT014420.1:CDS
TTCTCAAACTCAACCAAAGG+AGG 0.613689 3:-72838204 None:intergenic
GGGGCTTCCATCTGTTGCAG+TGG 0.616057 3:+72839186 MsaT014420.1:CDS
TAAGACGTGTCTTGGTCTGG+AGG 0.616442 3:+72840103 MsaT014420.1:CDS
TAGACGAAGGAAGGCGAAAG+GGG 0.617367 3:+72845357 MsaT014420.1:CDS
GAAAGGGGAACATGAAGCGT+AGG 0.617607 3:+72845372 MsaT014420.1:CDS
GATGGAAGCCCCACAACTAG+AGG 0.618341 3:-72839175 None:intergenic
CTTGGTATAAGGCATCCTGC+CGG 0.619801 3:+72839091 MsaT014420.1:CDS
AAGCGATGAATCAACTCTAA+GGG 0.620220 3:+72839626 MsaT014420.1:CDS
TTTATGATGTGAAAGGCAAG+AGG 0.620240 3:-72839685 None:intergenic
GGCGGAGCAACCAATTCATG+TGG 0.622069 3:+72845240 MsaT014420.1:intron
TTCGCCCAGAAAGCACAGTA+CGG 0.622172 3:-72839791 None:intergenic
AGATAAGAGCGATCTACCAT+CGG 0.623826 3:-72839928 None:intergenic
ATGTTTGTGGTATTCCACAA+TGG 0.624693 3:-72839236 None:intergenic
TCTAGACGAAGGAAGGCGAA+AGG 0.625198 3:+72845355 MsaT014420.1:CDS
ATCGGGTCATTGAGTAAACG+TGG 0.626423 3:+72838165 MsaT014420.1:CDS
GGCAGCGGCAGGAATTCAGA+TGG 0.629000 3:+72839585 MsaT014420.1:intron
TGAAATCTGGTACCAACAAG+AGG 0.629514 3:-72843227 None:intergenic
AAGTTCACAACTCTCAGAGT+CGG 0.630064 3:-72840869 None:intergenic
TGGCAAAACCGAATTTGGAG+AGG 0.630456 3:-72843896 None:intergenic
GTTGTAAATGATACGTGTGA+TGG 0.630651 3:+72838288 MsaT014420.1:CDS
CCACACAGTCATTTGCGTCA+AGG 0.632292 3:+72840002 MsaT014420.1:CDS
ACTGTGGTAGATACATGGAC+CGG 0.633569 3:+72843763 MsaT014420.1:CDS
GAAGTACGGAATAGAAACCC+TGG 0.641639 3:-72843012 None:intergenic
CTTCAGCTACCTTATCTCCA+AGG 0.642106 3:-72843202 None:intergenic
TTATTGTCCTCAAGCCATTG+TGG 0.643240 3:+72839222 MsaT014420.1:CDS
CGTGTGATGGAATGGAGGAG+AGG 0.644455 3:+72838301 MsaT014420.1:CDS
GTGGAGACACTTGATAATGG+AGG 0.645022 3:+72838548 MsaT014420.1:CDS
CATTTCTCAAACTCAACCAA+AGG 0.645043 3:-72838207 None:intergenic
GAAGCTCACGCAATTAGGAG+TGG 0.645058 3:+72843714 MsaT014420.1:intron
GCAAGATCACAAGTCTAAGG+AGG 0.646340 3:+72839968 MsaT014420.1:CDS
TGATACGTGTGATGGAATGG+AGG 0.646808 3:+72838296 MsaT014420.1:CDS
GTCCATGTATCTACCACAGT+GGG 0.654636 3:-72843760 None:intergenic
AAGTATCTCCTCTAGTTGTG+GGG 0.655937 3:+72839167 MsaT014420.1:CDS
CAAATCCCGCATCGACACGA+TGG 0.658482 3:-72840259 None:intergenic
ACAGCAACATGTTGTTACCA+AGG 0.659467 3:+72839736 MsaT014420.1:CDS
GACAAAACCATTCACCATCA+GGG 0.668378 3:-72840237 None:intergenic
TTTACCTGCTGTTCCCACTG+TGG 0.676231 3:+72843747 MsaT014420.1:CDS
TGGGTGCGGACAATCTAACA+TGG 0.678752 3:+72840830 MsaT014420.1:CDS
AAAGAAGTTCGCCTCCAAAG+CGG 0.687855 3:-72840052 None:intergenic
CCTTGACGCAAATGACTGTG+TGG 0.688106 3:-72840002 None:intergenic
CAAACCCTATCAATGGAGGA+GGG 0.690346 3:+72837545 None:intergenic
GGTCATTGAGTAAACGTGGG+AGG 0.693475 3:+72838169 MsaT014420.1:CDS
TGTTATCGACTGTGTCACCA+TGG 0.695665 3:+72844959 MsaT014420.1:CDS
TCGGGTCATTGAGTAAACGT+GGG 0.701785 3:+72838166 MsaT014420.1:CDS
GACTGGTGTACTCTAGACGA+AGG 0.706505 3:+72845344 MsaT014420.1:CDS
AGCCAAAGCAAATTCAACGG+GGG 0.735187 3:+72840283 MsaT014420.1:CDS
TAGCCAAAGCAAATTCAACG+GGG 0.739394 3:+72840282 MsaT014420.1:CDS
GGTCCATGTATCTACCACAG+TGG 0.749610 3:-72843761 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr3 gene 72837557 72845393 72837557 ID=MsaG014420
Chr3 mRNA 72837557 72845393 72837557 ID=MsaT014420.1;Parent=MsaG014420
Chr3 exon 72837557 72837622 72837557 ID=MsaT014420.1.exon1;Parent=MsaT014420.1
Chr3 CDS 72837557 72837622 72837557 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72838003 72838326 72838003 ID=MsaT014420.1.exon2;Parent=MsaT014420.1
Chr3 CDS 72838003 72838326 72838003 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72838450 72838605 72838450 ID=MsaT014420.1.exon3;Parent=MsaT014420.1
Chr3 CDS 72838450 72838605 72838450 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72838826 72838921 72838826 ID=MsaT014420.1.exon4;Parent=MsaT014420.1
Chr3 CDS 72838826 72838921 72838826 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72838968 72839285 72838968 ID=MsaT014420.1.exon5;Parent=MsaT014420.1
Chr3 CDS 72838968 72839285 72838968 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72839334 72839553 72839334 ID=MsaT014420.1.exon6;Parent=MsaT014420.1
Chr3 CDS 72839334 72839553 72839334 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72839586 72840124 72839586 ID=MsaT014420.1.exon7;Parent=MsaT014420.1
Chr3 CDS 72839586 72840124 72839586 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72840232 72840352 72840232 ID=MsaT014420.1.exon8;Parent=MsaT014420.1
Chr3 CDS 72840232 72840352 72840232 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72840510 72840595 72840510 ID=MsaT014420.1.exon9;Parent=MsaT014420.1
Chr3 CDS 72840510 72840595 72840510 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72840787 72841063 72840787 ID=MsaT014420.1.exon10;Parent=MsaT014420.1
Chr3 CDS 72840787 72841063 72840787 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72842993 72843074 72842993 ID=MsaT014420.1.exon11;Parent=MsaT014420.1
Chr3 CDS 72842993 72843074 72842993 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72843199 72843298 72843199 ID=MsaT014420.1.exon12;Parent=MsaT014420.1
Chr3 CDS 72843199 72843298 72843199 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72843715 72843922 72843715 ID=MsaT014420.1.exon13;Parent=MsaT014420.1
Chr3 CDS 72843715 72843922 72843715 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72844007 72844130 72844007 ID=MsaT014420.1.exon14;Parent=MsaT014420.1
Chr3 CDS 72844007 72844130 72844007 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72844923 72844980 72844923 ID=MsaT014420.1.exon15;Parent=MsaT014420.1
Chr3 CDS 72844923 72844980 72844923 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Chr3 exon 72845241 72845393 72845241 ID=MsaT014420.1.exon16;Parent=MsaT014420.1
Chr3 CDS 72845241 72845393 72845241 ID=cds.MsaT014420.1;Parent=MsaT014420.1
Gene Sequence

>MsaT014420.1

ATGGAGGAGGGTTTCGAGACTTCATCAAACTCGCAATCAAAGCTGAAAATCAATGAAAGAGTTGAGGTGAAGAGTTTTGAAGAGGGGTTTCTGGGATCATGGCATCCAGGGACAGTTATTGATAGTGAGAAGCTGAAGCGTCATGTTCGATATGAGAATATTCTGAATGATAATGGGTTGGGTAATTTTGTGGAGGTAGTGAGTGTTTCAAGTGTTTTGGATGGTGATATCGGGTCATTGAGTAAACGTGGGAGGATTAGGCCAGTGCCTCCTTTGGTTGAGTTTGAGAAATGTGATCTTAAGTATGGTTTGTGCGTTGATGTAAACTATCAGGAAGCTTGGTGGGAAGGTGTTGTAAATGATACGTGTGATGGAATGGAGGAGAGGACTTCGTATCTTCCGGTATCAGTAAGGCAAATTTGGTATGATGTGCGACAAAAAAGGAATTACGCGATTGGTGAATGGACATTGAATGATAAGAAGGATTTGTGGAGACACTTGATAATGGAGGTAGTTGGTGACTACTTGGCTGTTACAGTAAAGGAACGTGTAATATTGTCTGAAACTGAATACTGTCCCGATGCTGTTAAGCAATATGTGCTTGCTTTTGGCAAGGCAACTATGACATCTTTGAAGGAAAAAGGTGGTAAACAATACAGGTACAAGCCACCCAATGGAATCCATTGCAATAAGAAAGTTTACTACTCACTTATTGAACTTTGTAAAGCTGAATCCATGATGAACTCTTCTCCGTCCGAAAATGGTCTTGGTATAAGGCATCCTGCCGGTGACTGTCATGTTCCAAATGTGCCTCTTAGTCCATCTGAACAGATTCAGAACCAAGTATCTCCTCTAGTTGTGGGGCTTCCATCTGTTGCAGTGGCAGATGAACAAGCTTATTGTCCTCAAGCCATTGTGGAATACCACAAACATGCACTCAAGAAGACCTGCAATCGGGCAGATGGATTTCAATTGATCCACCTCCAAATAATAGAAGAAGGTGTTTTATACATTTCTCCACTGAAACAGAAATTCTTCTCATTGAATACAGTATGCAGTTTCTTACTTAAAGAAAGTATTCAGAAATCGACTATTTCTGGCATGCAACCTTTAAATACCTTGACCATTCATGAGGAAAATGTTGATCAGGTATTGAGTGATGATTTATCAAGTAGAGACAGCAGCGGCAGGAATTCAGATGGCAGCCTGCCAAAGCGCCGAAGCGATGAATCAACTCTAAGGGTACTGAGATCAAATGAAAGGGTGCCAAAGGCGTCTTCCTCTTGCCTTTCACATCATAAACCTTCAAATGTTCTGTCTTGGTTGATAGACAGCAACATGTTGTTACCAAGGTCTAAGGTTTTTTGTCGAGCAAAACGTGGGCACCGTACTGTGCTTTCTGGGCGAATAACTCATGAAGGCATCAAGTGTAACTGTTGTTCAAGAATATACAGTCTTGTTTCCTTCGAATTTCATGCTACTGGCAGTAATACCATCAGACCAAGTGCCAATATCTTTCTGGCCGATGGTAGATCGCTCTTATCTTGCCAGATACAAATAATGCAAGATCACAAGTCTAAGGAGGCTATGATAAAACCACACAGTCATTTGCGTCAAGGTGAAAACGACTACATTTGTTCCGTTTGCCGCTTTGGAGGCGAACTTCTTTTGTGTGATCAATGCCCGTCTTCATATCATAAGACGTGTCTTGGTCTGGAGGATCTCCCTGATGGTGAATGGTTTTGTCCATCGTGTCGATGCGGGATTTGTAGCCAAAGCAAATTCAACGGGGGTGGTGAGGATGAGCATTTCCTTACATGCATTCAGTGTGAACATAAATATCATGTTGCGTGCCTGAAAAGTAGAGATACCAGTAAATCCGGAAGTTATGTTGAAAGCCGGTTCTGCGGAAAAGACTGTGAAAAGTTATATACAGCCCTTCAAAGTCTGTTAGGACAGCCAGTTTCAGTGGGTGCGGACAATCTAACATGGACATTAGTGAAGTTTGCCGACTCTGAGAGTTGTGAACTTGATAATAGTAAAAGTGGATTATTGGCAGAAAGTTACAGCAAACTCAATGTTGCTCTTTCAGTGATGCATGAATGTTTGAGCCCTAAAGGAATCCTCTTCTTGCAGAGATCTGATGGAAAATGTTTTATTCAGCCGATGGTAAGAGTTTCATATCCATCAAAATATGTTCATGAACTTAATCGAATGAATTTCCAGGGTTTCTATTCCGTACTTCTGGAGAGAAATGAAGATCTGGTCAGTGTAGCAACTATTAGGGTCCTTGGAGATAAGGTAGCTGAAGTACCTCTTGTTGGTACCAGATTTCAGTATCGTCGTCATGGAATGTGTCGCATATTAATGGATGAATTGGAGAAGAAGCTCACGCAATTAGGAGTGGAACGTCTCGTTTTACCTGCTGTTCCCACTGTGGTAGATACATGGACCGGCGCTTTTGGATTTTCAAAGATGCCAAACTTTGAAAGATCACAATTTCTAAACTATACTTTCCTAGATTTTCCGGGTACCATCATGTGCCAGAAGTTTTTAGCAAATATTCCCTCTCCAAATTCGGTTTTGCCAACTGAGTTCCAACGAAAACAAGATGCTATCTCCGGAAGCAGTACTGCTAGTAATAAGTCTCCAGTTTCTGAAGTTTACCAAGCAGAAGAGATAGACAAAAGAGAATCGTTGGATCAACAAATGATGGATACTTCTGAAGGCGATAATGATAATCTTGGCAGTGCTGTTATCGACTGTGTCACCATGGCGGAGCAACCAATTCATGTGGACCAACAACATTGCCAGAATGAAACCACTCAAAAGTGCCAGAATGAAACCACTCAAAAGTGCTCTCTTGTAAAGTGGAGTGGACTGGTGTACTCTAGACGAAGGAAGGCGAAAGGGGAACATGAAGCGTAG
Protein sequence

>MsaT014420.1

MEEGFETSSNSQSKLKINERVEVKSFEEGFLGSWHPGTVIDSEKLKRHVRYENILNDNGLGNFVEVVSVSSVLDGDIGSLSKRGRIRPVPPLVEFEKCDLKYGLCVDVNYQEAWWEGVVNDTCDGMEERTSYLPVSVRQIWYDVRQKRNYAIGEWTLNDKKDLWRHLIMEVVGDYLAVTVKERVILSETEYCPDAVKQYVLAFGKATMTSLKEKGGKQYRYKPPNGIHCNKKVYYSLIELCKAESMMNSSPSENGLGIRHPAGDCHVPNVPLSPSEQIQNQVSPLVVGLPSVAVADEQAYCPQAIVEYHKHALKKTCNRADGFQLIHLQIIEEGVLYISPLKQKFFSLNTVCSFLLKESIQKSTISGMQPLNTLTIHEENVDQVLSDDLSSRDSSGRNSDGSLPKRRSDESTLRVLRSNERVPKASSSCLSHHKPSNVLSWLIDSNMLLPRSKVFCRAKRGHRTVLSGRITHEGIKCNCCSRIYSLVSFEFHATGSNTIRPSANIFLADGRSLLSCQIQIMQDHKSKEAMIKPHSHLRQGENDYICSVCRFGGELLLCDQCPSSYHKTCLGLEDLPDGEWFCPSCRCGICSQSKFNGGGEDEHFLTCIQCEHKYHVACLKSRDTSKSGSYVESRFCGKDCEKLYTALQSLLGQPVSVGADNLTWTLVKFADSESCELDNSKSGLLAESYSKLNVALSVMHECLSPKGILFLQRSDGKCFIQPMVRVSYPSKYVHELNRMNFQGFYSVLLERNEDLVSVATIRVLGDKVAEVPLVGTRFQYRRHGMCRILMDELEKKLTQLGVERLVLPAVPTVVDTWTGAFGFSKMPNFERSQFLNYTFLDFPGTIMCQKFLANIPSPNSVLPTEFQRKQDAISGSSTASNKSPVSEVYQAEEIDKRESLDQQMMDTSEGDNDNLGSAVIDCVTMAEQPIHVDQQHCQNETTQKCQNETTQKCSLVKWSGLVYSRRRKAKGEHEA*