Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014438 | XP_013460322.1 | 80.155 | 388 | 18 | 4 | 32 | 360 | 1 | 388 | 0.0 | 612 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014438 | sp|Q42381|HLS1_ARATH | 40.898 | 401 | 155 | 9 | 39 | 359 | 4 | 402 | 5.48e-89 | 276 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014438 | tr|A0A072UZ42|A0A072UZ42_MEDTR | 80.155 | 388 | 18 | 4 | 32 | 360 | 1 | 388 | 0.0 | 612 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG014438 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014438 | MtrunA17_Chr3g0105631 | 80.155 | 388 | 18 | 4 | 32 | 360 | 1 | 388 | 0.0 | 612 |
| MsaG014438 | MtrunA17_Chr4g0053961 | 40.759 | 395 | 150 | 8 | 48 | 359 | 17 | 410 | 2.14e-86 | 267 |
| MsaG014438 | MtrunA17_Chr5g0401351 | 49.416 | 257 | 112 | 4 | 39 | 278 | 11 | 266 | 8.92e-75 | 236 |
| MsaG014438 | MtrunA17_Chr5g0401351 | 47.674 | 86 | 41 | 2 | 275 | 360 | 322 | 403 | 6.75e-18 | 84.3 |
| MsaG014438 | MtrunA17_Chr4g0001411 | 48.235 | 255 | 120 | 6 | 39 | 285 | 25 | 275 | 1.07e-62 | 206 |
| MsaG014438 | MtrunA17_Chr4g0001411 | 43.750 | 96 | 36 | 4 | 275 | 359 | 320 | 408 | 4.59e-13 | 69.7 |
| MsaG014438 | MtrunA17_Chr4g0068331 | 34.804 | 408 | 173 | 16 | 35 | 359 | 9 | 406 | 2.84e-60 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG014438 | AT4G37580.1 | 40.898 | 401 | 155 | 9 | 39 | 359 | 4 | 402 | 5.57e-90 | 276 |
| MsaG014438 | AT2G30090.1 | 55.372 | 242 | 94 | 5 | 39 | 278 | 15 | 244 | 3.56e-83 | 258 |
| MsaG014438 | AT5G67430.1 | 38.889 | 396 | 159 | 11 | 34 | 360 | 5 | 386 | 5.96e-82 | 255 |
| MsaG014438 | AT2G23060.1 | 47.940 | 267 | 103 | 6 | 39 | 272 | 7 | 270 | 3.66e-72 | 230 |
| MsaG014438 | AT2G23060.1 | 51.765 | 85 | 37 | 2 | 275 | 359 | 332 | 412 | 3.33e-20 | 91.7 |
| MsaG014438 | AT4G37580.2 | 38.529 | 340 | 131 | 7 | 97 | 359 | 7 | 345 | 4.91e-66 | 213 |
| MsaG014438 | AT5G67430.2 | 36.778 | 329 | 131 | 9 | 101 | 360 | 1 | 321 | 1.50e-60 | 197 |
| MsaG014438 | AT2G23060.2 | 36.918 | 279 | 109 | 6 | 147 | 359 | 1 | 278 | 1.18e-51 | 173 |
Find 85 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAAAGATGATATTAATAT+TGG | 0.198020 | 3:-73193263 | MsaT014438.1:CDS |
| ATTAAGGAATAAATTAAGTT+TGG | 0.224040 | 3:-73193303 | MsaT014438.1:CDS |
| AGCTCTTGTAGGTGGGATTT+TGG | 0.230695 | 3:+73192797 | None:intergenic |
| CCTTTGGAATGAGGATGTTT+TGG | 0.266501 | 3:+73196389 | None:intergenic |
| CTATTTGGCACTTGTCCATT+AGG | 0.291316 | 3:+73193239 | None:intergenic |
| AATATATTTCCTATATCATT+AGG | 0.292377 | 3:+73193323 | None:intergenic |
| CTAAGCATAGCCCAACTATT+TGG | 0.310916 | 3:+73193224 | None:intergenic |
| TTAAGGAATAAATTAAGTTT+GGG | 0.313536 | 3:-73193302 | MsaT014438.1:CDS |
| ATGATATAGGAAATATATTA+AGG | 0.325837 | 3:-73193319 | MsaT014438.1:CDS |
| TTTATGCACCACAAATCTTC+AGG | 0.326456 | 3:+73192867 | None:intergenic |
| AGGACATCCACCAAAGGATT+TGG | 0.330187 | 3:-73193672 | MsaT014438.1:CDS |
| TTGGCATCAGTTGGAGCCTT+AGG | 0.339136 | 3:+73196408 | None:intergenic |
| ATGTCAATTTGTGCACAATA+TGG | 0.349042 | 3:-73192991 | MsaT014438.1:CDS |
| ATCATACTTTAAACCTCTCT+TGG | 0.350495 | 3:+73192762 | None:intergenic |
| GATATTAATATTGGGCCTAA+TGG | 0.352217 | 3:-73193254 | MsaT014438.1:CDS |
| AAGTTCCTTCCACTTAGATA+TGG | 0.354748 | 3:-73194418 | MsaT014438.1:CDS |
| AGTTTAACTACACAAAGTTT+AGG | 0.361536 | 3:-73193478 | MsaT014438.1:CDS |
| TTAAAGATGATATTAATATT+GGG | 0.372605 | 3:-73193262 | MsaT014438.1:CDS |
| TAGGGTATGCCCTAGGCTTA+AGG | 0.378312 | 3:-73193643 | MsaT014438.1:CDS |
| ATAAAAGTTGTAACTGTTCA+AGG | 0.382489 | 3:-73193692 | MsaT014438.1:CDS |
| GTTTCTCTTCACAGACACTA+TGG | 0.386937 | 3:-73194292 | MsaT014438.1:CDS |
| TCTCCACATCATAGAAGAAA+AGG | 0.405669 | 3:-73193617 | MsaT014438.1:CDS |
| ATCACTCTATAGAAGATTCA+TGG | 0.406094 | 3:-73193363 | MsaT014438.1:CDS |
| CCTACTGTTGCCAAATCCTT+TGG | 0.417451 | 3:+73193662 | None:intergenic |
| AGCAGAGCTTGACAATGAAT+TGG | 0.418408 | 3:-73193735 | MsaT014438.1:CDS |
| AAGATTATTGTGACTGAAGT+TGG | 0.419377 | 3:-73192942 | MsaT014438.1:CDS |
| TCTCATGCCCTGAAGATTTG+TGG | 0.422983 | 3:-73192875 | MsaT014438.1:CDS |
| ATTCTTCTAATGTTCTTACT+AGG | 0.427168 | 3:+73193582 | None:intergenic |
| TGAAGATTTGTGGTGCATAA+AGG | 0.428454 | 3:-73192865 | MsaT014438.1:CDS |
| CAAACAACATTGAAATATCT+AGG | 0.430645 | 3:-73193406 | MsaT014438.1:CDS |
| CTATGGGTGACCCCATTTGT+AGG | 0.434012 | 3:-73194275 | MsaT014438.1:CDS |
| ATAAATTAAGTTTGGGGACA+TGG | 0.434467 | 3:-73193295 | MsaT014438.1:CDS |
| GAATTGGTTGGTGTCATTCA+AGG | 0.456359 | 3:-73193719 | MsaT014438.1:CDS |
| ATGGACAAGTGCCAAATAGT+TGG | 0.459933 | 3:-73193235 | MsaT014438.1:CDS |
| GTATGCATAGTCCACATCAT+TGG | 0.466746 | 3:+73193550 | None:intergenic |
| ACTGTTTCGAATCCTACAAA+TGG | 0.469226 | 3:+73194263 | None:intergenic |
| TCATACTTTAAACCTCTCTT+GGG | 0.469463 | 3:+73192763 | None:intergenic |
| TTTAATGAGTGATGGTTCAC+AGG | 0.470283 | 3:+73193434 | None:intergenic |
| AGGGTATGCCCTAGGCTTAA+GGG | 0.471150 | 3:-73193642 | MsaT014438.1:CDS |
| CTGTTTCGAATCCTACAAAT+GGG | 0.471230 | 3:+73194264 | None:intergenic |
| GTTGGGCTATGCTTAGTGTA+TGG | 0.480182 | 3:-73193217 | MsaT014438.1:CDS |
| ACTGTTGCCAAATCCTTTGG+TGG | 0.486849 | 3:+73193665 | None:intergenic |
| GCATCAGTTGGAGCCTTAGG+TGG | 0.488623 | 3:+73196411 | None:intergenic |
| TGGACAAGTGCCAAATAGTT+GGG | 0.496162 | 3:-73193234 | MsaT014438.1:CDS |
| AAATGCTCACCTTTGGAATG+AGG | 0.509103 | 3:+73196380 | None:intergenic |
| TAGTAAGAACATTAGAAGAA+TGG | 0.512758 | 3:-73193580 | MsaT014438.1:CDS |
| ACAGGGTTAACAAGAATAGA+TGG | 0.514152 | 3:+73193452 | None:intergenic |
| TTATGCACCACAAATCTTCA+GGG | 0.517119 | 3:+73192868 | None:intergenic |
| GTTTAGAATCAGAAGCTATG+AGG | 0.517776 | 3:-73194385 | MsaT014438.1:CDS |
| ATATACCAGCATCATGTACA+AGG | 0.526000 | 3:+73194239 | None:intergenic |
| TCACTCTATAGAAGATTCAT+GGG | 0.529453 | 3:-73193362 | MsaT014438.1:CDS |
| CATCAGTTGGAGCCTTAGGT+GGG | 0.533970 | 3:+73196412 | None:intergenic |
| TGTTGAATTCCATATCTAAG+TGG | 0.537968 | 3:+73194409 | None:intergenic |
| CTCCACATCATAGAAGAAAA+GGG | 0.538419 | 3:-73193616 | MsaT014438.1:CDS |
| ATGGTTCATTTCCAATGATG+TGG | 0.539215 | 3:-73193561 | MsaT014438.1:CDS |
| TTTCTCTTCACAGACACTAT+GGG | 0.544269 | 3:-73194291 | MsaT014438.1:CDS |
| GTGGAGATACCCTTAAGCCT+AGG | 0.544733 | 3:+73193633 | None:intergenic |
| ATGCTTAGTGTATGGAATAG+TGG | 0.546161 | 3:-73193209 | MsaT014438.1:intron |
| ATTAAGTTTGGGGACATGGA+TGG | 0.547711 | 3:-73193291 | MsaT014438.1:CDS |
| CAGTCCCTTGTACATGATGC+TGG | 0.551002 | 3:-73194244 | MsaT014438.1:intron |
| CATCATAGAAGAAAAGGGAT+TGG | 0.551271 | 3:-73193611 | MsaT014438.1:CDS |
| TAAGGAATAAATTAAGTTTG+GGG | 0.567784 | 3:-73193301 | MsaT014438.1:CDS |
| CAAAGGATTTGGCAACAGTA+GGG | 0.570122 | 3:-73193661 | MsaT014438.1:CDS |
| CCAAAGGATTTGGCAACAGT+AGG | 0.570760 | 3:-73193662 | MsaT014438.1:CDS |
| TTTAGAATCAGAAGCTATGA+GGG | 0.576337 | 3:-73194384 | MsaT014438.1:CDS |
| CAATGTTAAAATCCCACCTA+AGG | 0.578225 | 3:-73196424 | MsaT014438.1:CDS |
| TTAGAGAGAAAATGTGAGGT+AGG | 0.580395 | 3:-73194330 | MsaT014438.1:CDS |
| ATTATTGTGACTGAAGTTGG+AGG | 0.580952 | 3:-73192939 | MsaT014438.1:CDS |
| TGGAGATACCCTTAAGCCTA+GGG | 0.588065 | 3:+73193634 | None:intergenic |
| GCAACAGTAGGGTATGCCCT+AGG | 0.591773 | 3:-73193650 | MsaT014438.1:CDS |
| TGATGTGGACTATGCATACA+TGG | 0.605779 | 3:-73193546 | MsaT014438.1:CDS |
| GAATTCCATATCTAAGTGGA+AGG | 0.613396 | 3:+73194413 | None:intergenic |
| CCAAAACATCCTCATTCCAA+AGG | 0.615090 | 3:-73196389 | MsaT014438.1:intron |
| TTAATGAGTGATGGTTCACA+GGG | 0.619510 | 3:+73193435 | None:intergenic |
| GAGCTTGACAATGAATTGGT+TGG | 0.628667 | 3:-73193731 | MsaT014438.1:CDS |
| AAGAGAAACACACTTTCTGA+TGG | 0.631909 | 3:+73194306 | None:intergenic |
| TCAATCATCACATTCCACAT+TGG | 0.637209 | 3:-73192905 | MsaT014438.1:CDS |
| AAATTTAGAGAGAAAATGTG+AGG | 0.640564 | 3:-73194334 | MsaT014438.1:CDS |
| CTAAGTGGAAGGAACTTGAG+AGG | 0.656072 | 3:+73194424 | None:intergenic |
| GTTCATGAAGAGATTGACTG+AGG | 0.679343 | 3:+73193502 | None:intergenic |
| CATGAGAGCAACTTCCAATG+TGG | 0.679406 | 3:+73192891 | None:intergenic |
| TGTTCAAGGACATCCACCAA+AGG | 0.683056 | 3:-73193678 | MsaT014438.1:CDS |
| TATACCAGCATCATGTACAA+GGG | 0.703192 | 3:+73194240 | None:intergenic |
| CACTCTATAGAAGATTCATG+GGG | 0.722156 | 3:-73193361 | MsaT014438.1:CDS |
| TGTTTCGAATCCTACAAATG+GGG | 0.724283 | 3:+73194265 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 73192770 | 73196444 | 73192770 | ID=MsaG014438 |
| Chr3 | mRNA | 73192770 | 73196444 | 73192770 | ID=MsaT014438.1;Parent=MsaG014438 |
| Chr3 | exon | 73192770 | 73193032 | 73192770 | ID=MsaT014438.1.exon4;Parent=MsaT014438.1 |
| Chr3 | CDS | 73192770 | 73193032 | 73192770 | ID=cds.MsaT014438.1;Parent=MsaT014438.1 |
| Chr3 | exon | 73193210 | 73193759 | 73193210 | ID=MsaT014438.1.exon3;Parent=MsaT014438.1 |
| Chr3 | CDS | 73193210 | 73193759 | 73193210 | ID=cds.MsaT014438.1;Parent=MsaT014438.1 |
| Chr3 | exon | 73194245 | 73194459 | 73194245 | ID=MsaT014438.1.exon2;Parent=MsaT014438.1 |
| Chr3 | CDS | 73194245 | 73194459 | 73194245 | ID=cds.MsaT014438.1;Parent=MsaT014438.1 |
| Chr3 | exon | 73196390 | 73196444 | 73196390 | ID=MsaT014438.1.exon1;Parent=MsaT014438.1 |
| Chr3 | CDS | 73196390 | 73196444 | 73196390 | ID=cds.MsaT014438.1;Parent=MsaT014438.1 |
| Gene Sequence |
| Protein sequence |
